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http://doi.org/10.5281/zenodo.1053858
Please cite this article in press as Seeram Hariprasad et al., Photo Kinetic Studies of Methyl Orange Dye By Using
Green Synthesiszed Silver Nanoparticles from Areva Lanata Stem Extract, Indo Am. J. P. Sci, 2017; 4(11).
INTRODUCTION:
Metallic nanoparticles play major role in the field of paper industries. They are non-degradable and
nanomedicine and Nanotechnology because of their carcinogenic agents. Moreover, to unique in their
variety and large applications. nanoparticles used in products most of the industries uses colour dyes,
almost all fields. Several methods have been used for without any treatment the coloring material are
the synthesis of nanoparticles. But green method is liquidated in water leads to contamination of
best suitable method. Due to nontoxic chemicals resources [15]. The release of dyes effluents in
were not used. aquatic system is major environmental concern
because coloration not only decreases sunlight
Recent results were found to synthesis of penetration and toxic compounds during chemical or
nanoparticles and their photo degradation effect of biological reaction pathway that effects aquatic flora
dyes are studied from Amaranthus gangeticus Linn and fauna [16].
(Chinese spinach) leaf extract [1], Vishanika or In this present study, we discuss photo catalytic
Indian screw tree [2], Hypnea musciformis [3], Ulva degradation of green synthesized silver nanoparticles
lactuca (seaweed) [4], the leaf extract of Neem [5], against methyl orange dye.
aqueous extract of SDKP [6], brown seaweed Padina
tetrastromatica leaf extract [7], A.niger [8], Brassica MATERLS AND METHODS:
oleracea capitata (Cabbage) [9], Zanthoxylum All the chemical were used in this experiment are
armatum [10], Casuarina equisetifolia leaf sigma Aldrich.
extract[11], tangerine peel extract [12], bacterial
[13]. Preparation of Silver Nano Particles:
Preparation method and characterization of green
Dyes are belongs to organic compounds [14]. Dyes synthesized silver nanoparticles was already reported
are mainly used as a coloring agent in textile and [17].
Areva lanata extract is prepared by 10 gm of dried solution of the dye is taken out in 250 ml clean and
leaves are taken in clean and dry conical flask. dry beaker. To this 2 mg of green synthesized silver
To it add 100 ml of double distilled de ionized water. nanoparticles is added. The solution mixture is
The colour of the extract is light brown. 10 ml of exposed to sun light for about 180 min. Experiments
leaves extract is added to 90 ml of 1 mM silver are done between 10 am to 2 pm (temperatures 35-
nitrate solution. the colour of solution is changes 39oC).
from light brown to dark brown, indicates the
formation of silver nanoparticles. the reaction In every 30 minutes of time intervals, 5 ml of
mixture incubated 24 hours in dark room at room reaction mixture is taken out into centrifuging tubes
temperature. The mixture is centrifuged 20 minutes at and centrifuged, after that the filtrate is studied to
10,000 RPM and washed double distilled de ionized monitor the absorption maximum values using UV-
water, dried at 600 C. visible spectrophotometer. The readings were noted
in Table (4.3). Before exposure to sun light the
Characterization: aqueous Methyl orange solution gives UV-visible
UV-Visible absorption spectra are measured suing absorption maximum value at 464nm. The orange
shimdzu uv-2203 doublem beam spectrophotometer. colour of the solution is found to slowly decolourise
FTIR spectra are obtained with IR-prestige- within an exposure time of 180 min. the reported
21shimaduz, FTIR spectrophotometer, using Kbr values for degradation correspond to the maximum
pellet method. SEM studies of silver nanoparticles rate of degradation, after which the degradation rate
are done by using jsm-6610lv machine. The slow down taking very long time for complete
morphology of silver nanoparticles is done by TEM degradation. This may be due to saturation of the
analysis, by using x-pert pro machine. adsorption sites of the nanoparticles surface.
67.5
%T
60
52.5
45
37.5
30
22.5
15
4694.94
4670.83
4650.57
4611.03
4530.98
4485.65
4470.22
4447.08
4428.75
4362.20
4303.37
4287.94
4096.98
4070.94
4054.55
4036.22
4018.86
4001.50
3985.10
3966.78
3938.81
3912.77
3892.52
3879.02
3861.66
3845.26
3828.86
3810.54
3790.29
3776.78
3762.32
3741.10
3721.81
3699.63
3682.27
3660.09
3637.90
3620.54
3577.14
3555.92
3534.71
3514.45
3491.31
3452.73
3430.55
3281.06
3263.70
3253.09
3237.66
3224.15
3207.76
3092.02
3076.59
2984.01
2957.97
2926.14
2360.97
2339.75
2011.84
1982.91
1934.69
1914.43
1879.71
1858.49
1837.28
1819.92
1784.23
1755.30
1740.83
1725.40
1707.08
1659.82
1594.23
1569.16
1549.87
1531.55
1516.11
1497.79
1483.32
1465.96
1445.71
1383.02
1367.59
1354.09
1306.83
1177.59
1147.69
1046.43
1016.53
831.36
768.67
717.55
682.83
668.36
609.53
588.31
524.66
498.62
481.26
465.83
452.33
431.11
408.93
386.74
366.49
347.20
4500 4000 3500 3000 2500 2000 1500 1000 500
1 1/cm
80
60
Weight%
40
20
0
C O Ag
.
Fig. 8: TEM image [17]
Fig. 10: Color change of methyl orange dye after addition of silver nano particles.
From the graph, it is clearly indicated that the photo degradation of the dye follows pseudo first order reaction The
rate constant of the degradation of methyl orange reaction by using green synthesized silver nanoparticles (2mg) is
calculated by following equation.
K = (2.303 M) /60
K= (2.3030.008)/ 60
K=3.0706 10-4 Sec-1
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