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INTRODUCTION
were completely dependent on medicinal herbs for prevention and the treatment
of diseases. Herbal remedies have been used for decades and centuries, the use
of medicinal herbs for curing diseases has been documented. The drugs were
used in crude forms like decoction, infusion, tincture, and poultice. The ancient
people who were below the poverty line and who cannot afford the high cost of
commercial synthetic medicines from the drugstores and opted to the use of local
plants within their reach. These plants can be found around their house yards,
rice fields, gardens, roadsides and rivers. These plants can be obtained readily,
cheaply, and entail easily prepared and application. (Racadio et.al, 2008)
Since time immemorial, people had been using herbal medicines. With the
advent of modern medicine and the development of new drugs, people had
manufactured from the flora and fauna that abound. However, the availability and
abundance of some of these sources had declined due to some natural and
people still resort to herbal medicine. In the ancient and medieval times, almost
all medicines came from plants. Plants were used to ease pain, heal wounds and
cure fever. Today, a lot of people still rely on medicinal plants to meet the
pudica Linn it is locally known as bain bain in Iloko and Makahiya in tagalog.
Third plant is the main subject of this study. Makahiya is very common and
weed and the leaf of Makahiya folds inward and droop when touched or shaken.
The stem is erect in young plants, but becomes touched creeping or trailing with
age. The stem is slender, branching, and it sparsely to densely prickly, growing
to a length of 1.5m. The leaves also are bipinnately compound, with one or two
pinnae pairs and 10-26 leaflets per pinna. The petioles are also prickly.
Pedunculated (stalked) pale pink or purple flower heads arise from the leaf axils.
The makahiya was found out that the extract contains a trace of alkaloids
since slight opaqueness was produced. Flavonoids are also present. The extract
also yielded positive with the triterpenes. The presence of these phytochemicals
in makahiya leaf extract has curative effects and therefore can be used as
alternative medicine.
It can also be deduced that the plant could be a potential source of many
activity. These may be useful for improving blood circulation in brain and in
Theoretical framework
The Philippine council for health research and development, the agency
that coordinates and helps fund studiest on alternative medicine among many
others. Executive director Jaime Montoya has a list of eight new on-going
research projects, four involve plants in the old test list (Sambong Ulisimang
beta, Tsaang-gubat and Bayabas, Golden Shower tree, Saluyot, Makahiya and
Guyabano). (Inquirer.net.PhilippineNewsforFilipinos).
This study was carried against the following test organisms: Staphylococcus
metagrophytes. She showed that out of the twelve plants tested seven (7) were
extract ranging from 100-2,500 g/ml. it was concluded that plant acids, tannins
reducing substance and flavonoids were present in plants, and asa potential
Makahiya plant was analyzed and concluded that the plant contain active
aeruginosa, and Candida albicans were used in this study to determine the
most especially to their infectious characteristics. These were tested for their
reaction in terms of growth and inhibition with the plant extract in most types of
coccus that forms cluster. It is now considered as the most common cause of
Candida albicans is oftn present in the skin, mouth, vagina, and intestinal
tract of healthy prsons and animals where it resides without cause diseases.
Besides its many uses and application, Bacillus subtilis has become the model
A skin lotion is a pseudo-stable mixture of oils and water. The oils use to
changing the feel, attracting water to the skin, and blocking water from escaping
the skin. When put on the skin, lotion interact with skin proteins and immediately
sooths dry feeling hands. Over time, the occlusive agents in the lotion prevent
water from leaving the skin which helps build moisture in the surface. This
additional moisture ultimately mitigates the problems associated with dry skin.
(Romanowski, 2017)
growth of Staphylococcus aureus which causes infection in the skin. The efficacy
and safety of the formulation will depend on the inhibition of the growth of
Makahiya leaves will be thoroughly washed with clean running water to remove
the dirt present in the fresh leaves of Makahiya. We will be using a maceration
method as the extraction process. The use of the process for preparations
placed or permitted to soak in a solvent for specific period of time until the
constituents are dissolved and extracted out. The extract of Makahiya leaves
obtained will be the active ingredient I the formulation of Makahiya lotion, which
may be responsible in inhibiting the growth of Staphylococcus aureus. Addition of
in-vitro test to determine the zone of growth of inhibition of the formulated lotion
on Mannitol Salt Agar. Mannitol Salt agar is a commonly used selective and
an acidic by product is formed that causes the phenol red in the agar to turn
The Makahiya lotion that has been formulated will then be evaluated to its
important extra measure of protection for consumers at home and doctors and
This study intends to formulate a lotion using the extract of the Mimosa pudica
materials or plant parts used, this study has been limited to theoretical evaluation
inhibiting the growth Staphylococcus aureus. It also focuses on the gathering and