Vitamin D intragastric

C57BL/6 tikus itu dibeli dari Vital River Laboratory Animal Technology Co. Ltd untuk in
vivo studi [License No. SCXK (Beijing) 2006-0009]. Kolitis akut diinduksi oleh pemberian
air minum dextran natrium sulfat (DSS; 40 000-50 000 MW; Sigma). Tikus jantan usia 8
minggu menerima air minum biasa (kontrol) atau 2% (w/v). DSS air minum (model) ad
libitum selama 7 hari, setelah itu dilanjutkan air minum biasa selama sisa masa percobaan.
Total 30 tikus diambil secara acak untuk kontrol, model dan 1,25 (OH) 2D3treated group
(masing-masing kelompok = 10). Tikus di kelompok 1,25(OH)2D3 menerima 1,25 (OH) 2D
3 (Sigma) setiap hari (0.2 ug 25 g/d) dengan pemberian intragastric untuk 14 hari, dan tikus
dalam kelompok kontrol dan model diberi normal saline tanpa DSS.

C57BL/6 mice were purchased from Vital River Laboratory Animal Technology Co. Ltd for
in vivo studies [License No. SCXK (Beijing) 2006-0009]. Acute colitis was induced by
administration of dextran sodium sulfate (DSS; 40 000-50 000MW; Sigma) drinking water.
Male mice of 8weeks received either regular drinking water (control) or 2% (w/v) DSS
drinking water (model) ad libitum for 7days, after which the mice were resumed on water for
the remainder of the experiment. A total of 30 mice were randomly assigned to control, model
and 1,25(OH)2D3treated group (each group=10). The mice in the 1,25(OH)2D3treated
group received 1,25(OH)2D3 (Sigma) daily (0.2 ug/25g/d) by intragastric administration for
14days [29], and the mice in control and model group were given normal saline without DSS.
Zhao, H., Zhang, H., Wu, H., Li, H., Liu, L., Guo, J., Zhang, X. (2012). Protective role of
1,25(OH)2vitamin D3 in the mucosal injury and epithelial barrier disruption in DSS-induced acute colitis
in mice. BMC Gastroenterology, 12, 57.

Pemberian intraperitoneal

Crystalline 1,25(OH)2D3 (SAFC Pharma, Madison, WI) was dissolved in RNA-grade ethanol
at 0.025 mg/ml and stored at 20C. The 1,25(OH)2D3 stock was diluted in sterile PBS
immediately prior to injection. Mice were administered either vehicle (PBS with 0.12%
ethanol) or 10 ng 1,25(OH)2D3 (0.5 g/kg) via intraperitoneal injection daily for up to 11 days.
The 1,25(OH)2D3 dose was chosen on the basis of previous in vivo studies with mice (32). For
additional dose-response studies, mice were administered 5 ng or 15 ng 1,25(OH)2D3 daily.
Kristal 1,25 (OH) 2D 3 (SAFC Pharma, Madison, WI) ini dilarutkan dalam RNA-grade
etanol pada 0.025 mg/ml dan disimpan di 20 C. 1,25 (OH) 3 2D diencerkan dalam steril
PBS sebelum dilakukan Injeksi. Tikus yang diberikan (PBS dengan 0,12% etanol) atau 10
ng 1,25 (OH) 2D 3 (0,5 g/kg) melalui intraperitoneal injeksi setiap hari selama 11 hari.
Dosis 1,25 (OH) 2D 3 dipilih berdasarkan studi di vivo sebelumnya dengan tikus (32). Untuk
kajian dosis respon tambahan, tikus yang diberikan 5 ng atau 15 ng 1,25 (OH) 2D 3 setiap
hari.
Ryz, N. R., Patterson, S. J., Zhang, Y., Ma, C., Huang, T., Bhinder, G., Vallance, B. A. (2012). Active
vitamin D (1,25-dihydroxyvitamin D3) increases host susceptibility to Citrobacter rodentium by
suppressing mucosal Th17 responses. American Journal of Physiology - Gastrointestinal and Liver
Physiology, 303(12), G1299G1311.