Académique Documents
Professionnel Documents
Culture Documents
Introduction
Limitations of conventional periodontal diagnosis
Advances In Clinical Diagnosis
Advances In Radiographic Assessment
Advances Microbiologic Analysis
Advances In Characterizing The Host Response
Introduction
advanced
Periodontal disease Gingival Inflammation
Periodontal Destruction
clinical radiographic
attatchment loss bone loss
Periodontal
pathogens
Host
Genetic
response
systemic behavioural
Consideration should be given microbiologic
immunologic
systemic
genetic
behavioural factors
in addition to clinical and radiographic parameters.
The focus is now disease prevention, early discovery, and
intervention to minimize treatment, thus enabling the most
desirable outcomes.
Computerized images.
Image property almost equal to conventional radiographs
1/3rd to half reductions in radiation dose.
Substraction Radiography
selective non-selective
Advantage: 1. clinician can obtain absolute or relative count
2. invitro method for antibiotic susceptibility
Limitations :
It employs Antibody
that recognize
specific bacterial antigen
Various procedures:
a. Direct & indirect immunofluorescence assays
b. Flow cytometry
c. ELISA
d. Membrane assay
e. Latex agglutination
Immunofluorescence assay
Advantages:
Simple and Rapid testing
Higher sensitivity and specificity.
Enzymatic methods
Disadvantage:
May be positive in clinically healthy site
Cannot detect disease activity
Limited organisms detected
Other pathogens may be present if its negative.
Molecular Biology Techniques
Hydroxyproline
Glycosaminoglycans
For periodontal diagnosis, the ideal diagnostic test should be
Quantitative.
Highly sensitive method capable of analyzing a single
periodontal site in health as well as disease.
Reproducible.
Highly specific.
Simple to perform.
A rapid, one or two stage procedure.
Non-invasive.
Versatile in terms of sample handling, storage and transport.
Amendable to chairside use.
Economical. ( Chapple 1997)
Chairside periodontal test kits can be categorized as
Microbiologic tests
Biochemical test kits
Genetic kits