Zinc

DR 900 Analytical Procedure AP-15/900

SPECIFICATIONS
Method: Colorimetric (DR 900)
Range: 0.02 to 3.00 ppm of Zn
Testing Time: 10 minutes

DESCRIPTION
Zinc and other metals in the sample are complexed
with cyanide. The addition of ZN-6 Reagent causes
the selective release of zinc. The zinc then reacts
with zincon (2-carboxy-2-hydroxy-5-sulfoforma-
zylbenzene) indicator. The resulting blue color is
proportional to the zinc concentration.

ORDERING INFORMATION
Order all replacement parts and reagents by their Nalco DR 900 Colorimeter
part numbers. To be sure this product is compatible
with your treatment program, contact your local
Nalco representative. Items marked with an *
are included with the kit or set. To place your
order, please contact your local customer service Optional Parts and Reagents
department.
Description Size Part No.
1:1 HCl 1.7 oz/50 mL 460-S0726.71
REPLACEMENT PARTS AND REAGENTS 1:1 HCl 3.4 oz/100 mL 460-S0726.72
Required Parts and Reagents 1:1 HCl 16.9 oz/500 mL 460-S0726.74
1:1 HCl 34.8 oz/1 L 460-S0726.75
Zinc 20 mL Reagent Set, 100 tests 460-P3103.88 1:1 HCl 1.06 gal/4 L 460-S0726.77
Includes: H-6** 8.5 oz/250 mL 460-S0279.73
ZN-5 Reagent Powder Pillows * H-6** 16.9 oz/500 mL 460-S0279.74
(20 mL sample size) 100/pkg H-6** 34.8 oz/1 L 460-S0279.75
ZN-6 Reagent (3.4 oz/100 mL)* H-6** 1.06 gal/4 L 460-S0279.77
**Use this solution as source of sodium hydroxide before *
Description Part No.
disposal. See Safety Precaution Section.
Nalco DR 900 Colorimeter 400-P0900.88
Cylinder, graduated, mixing,
glass, 25 mL 500-P1204.88
Pipet, disposable, 1 mL 500-P2107.88
Sample cells, 10-20-25 mL
w/cap, 6 pkg 500-P2555.88
Description Part No. SUPPORT
Pocket pH Meter 400-P1334.88 If you have any questions regarding this procedure,
pH (3-color) test strips please contact your Nalco representative. In North
(0-14 pH) (100/box) 500-P3204.88 America you can also contact the Equipment Help
pH (3-color) test strips Desk at 630-305-CHEM.
(5-10 pH) (100/box) 500-P3207.88
47mm filter membrane,
0.45 micron (100/pkg) 500-P1405.88
INTERFERENCES
Stopwatch 500-P1612.88
The following may interfere when present in
concentrations exceeding those listed below.
SAFETY PRECAUTIONS
1 mg/L = 1 ppm
1. Refer to the Material Safety Data Sheet (MSDS)
for specific solution safety information. Aluminum 6 ppm
Cadmium 0.5 ppm
2. IMPORTANT! ZN-5 Reagent contains cyanide
and is poisonous if taken internally or if fumes Copper 5 ppm
are inhaled. Do not add to an acidic sample. Iron (ferric) 7 ppm
Store away from water and acids. Keep away
Manganese 5 ppm
from children.
Nickel 5 ppm
3. When disposing ZN-5 Reagent, use good
Organic material Large amounts may interfere. Per-
ventilation or a fume hood. Proceed as follows:
form the mild digestion to eliminate
a. Add the waste (while stirring) to a beaker this interference.
containing a strong solution of sodium Highly buffered May exceed the buffering capacity of
hydroxide and calcium hypochlorite or sodium samples or extreme the reagents and require sample
hypochlorite (household bleach). The pH of sample pH pretreatment.
final solution must be greater than 10.
b. Maintain a strong excess of hydroxide and
hypochlorite and let the solution stand for
24 hours.
c. Flush the solution down the drain with a large
excess of water.
ZINC DR 900 PROCEDURE
Special Note: Adequate ventilation is required for this test procedure. When possible, this test should be run in a fume hood.
Start
1 2
Start Program 780 zinc (Zn).
Note: Total zinc deter-
mination needs a prior
digestion.
Fill a sample cell with 20 mL
of sample.
Note: Rinse with 1:1
HCl (460-S0726) and
demineralized water
before use.
CAUTION!
If the sample pH is
below 4, adjust the pH
to 4 to 5 with sodium
hydroxide (460-S0279)
before proceeding to
Step 4. Failure to follow
this precaution may lead
to the evolution of toxic
fumes.
3 4
Add the contents of one Measure 10 mL of the
ZN-5 Reagent Powder orange solution from Step 4
Pillow. Cap. Invert several into another sample cell
times to completely dissolve (the blank).
the powder.
Notes: Inconsistent
readings may result in low
zinc concentrations if all
the reagent particles are
not dissolved.
At this point the sample
color should be orange. If
the color is brown or blue,
dilute the sample and
repeat the test. Either the
zinc concentration is too
high, or an interfering metal
is present.
CAUTION!
This reagent contains
cyanide and is very
poisonous if taken
internally or if the fumes
are inhaled. Do not add
to an acidic sample.
Store away from water
and acids.
 5 6
Add 0.5 mL of ZN-6 Reagent
to the remaining orange
solution in the first sample
cell (the prepared sample).
Note: Use a plastic
dropper, as rubber bulbs
may contaminate the ZN-6
Reagent.
Tightly cap the cell. Shake
vigorously for 30 seconds
(the prepared sample).
Note: The sample color will
be reddish-orange, brown
or blue, depending on the
zinc concentration.
7 8
Start: TIMER 2 Place the blank into the
cell holder. Tightly cover
A 3-minute reaction period the sample cell with the
will begin. instrument cap.
Note: Steps 8-10 must
be completed within 10
minutes after the timer
beeps.

Zero Read

9 10 11
Press: ZERO Immediately place the Press: READ
prepared sample into the
The cursor will move to the The cursor will move to the
cell holder. Tightly cover
right, then the display will right, then the result in mg/L
the sample cell with the
show: Zn will be displayed.
instrument cap.
0.00 mg/L Zn
Note: Determine a reagent
blank for each lot of
reagent by running the
procedure on deionized
water. Subtract this value
from all following results
obtained in Step 11.

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