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2. Which one is the segment of a eukaryotic gene that is not represented in the
mature m-RNA ?
a. Intron
b. Exon
c. Plasmid
d. TATA box
5. Which is the enzyme that makes a double stranded DNA copy from a single
stranded RNA template molecule?
a. DNA Polymerase
b. RNA Polymerase
c. Reverse Transcriptase
d. Phosphokinase
10. Which one of the following is true for Nitric Oxide Synthase?
a. It is inhibited by Ca++
b. It catalyzes a dioxygenase reaction.
c. It requires NADPH, FAD, FMN and Heme iron.
d. It accepts electrons from NADH.
11. Which one of the following amino acid is responsible for elasticity of the corneal
layer of skin?
a. Histidine
b. Lysine
c. Cysteine
d. Keratin
14. Which one of the following is best parameter to monitor Thiamine level?
a. Transketolase level in blood
b. Thiamine level in blood
c. Reticulocytosis
d. G6PD activity
15. Fluorescamine is used to detect:
a. Fatty acids
b. Glucose
c. Amino acids
d. All
18. At higher temperature, why most of the mammalian enzymes become inactive?
a. Cleavage of peptide bonds
b. Loss of tertiary structure
c. Loss of sulfhydryl groups
d. Liberation of amino acids
20. In competitive inhibition the relation between Km and Vmax is one the following:
a. Km and Vmax are the same
b. Km and Vmax decreases
c. Km increases and Vmax remains the same
d. Km decreases and Vmax increases.
23. Supplementation of Vitamin B12 and folic acid in megaloblastic anemia leads to
the improvement of anemia due to:
a. Increased Haemoglobin production
b. Increased DNA synthesis in bone marrow
c. Increased iron absorption
d. Erythroid hyperplasia
1. ans is D ( Primer)
RNA polymerase does not require a primer to initiate transcription while
DNA polymerase requires it.
RNA polymerase holoenzyme requires the following:
Template of double stranded DNA
Four ribonucleotide triphosphates GTP, ATP, UTP, CTP
Mn2+ or Mg2+
2. ans is A ( Introns )
The primary RNA transcripts contain two types of sequences:
Introns (intervening sequences): sequences that do not code/ contribute to the
genetic information ultimately translated into the amino acid sequences of
protein molecule
Exons (expressed sequences): sequences that code for various amino acids.
This primary transcript undergoes extensive processing which involves
cleaving out of the intervening introns (non coding sequence) and splicing
(ligation) together of the exons (coding sequences).
Thus introns are removed from the primary transcript and exons are ligated
together to form mRNA molecule.
4. ans is D (Selenium)
Selenium in the form of selenocysteine is a component of enzyme glutathione
peroxidase.
Glutathione peroxidase helps to protect proteins, cell membranes, lipid and
nucleic acids from oxidant molecules.
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