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Forensic Science International 136 (2003) 92–95

Announcement of population data


Allele frequencies of 15 STR loci using AmpF/STR
Identifiler kit in a Korean population
Yoo-Li Kim, Ji-Yeon Hwang, Yoo-Jin Kim, Seok Lee, Nak-Gyun Chung,
Hyun-Gyung Goh, Chun-Choo Kim, Dong-Wook Kim*
Molecular Hematology Laboratory, Catholic Hematopoietic Stem Cell Transplantation Center,
The Catholic University of Korea, 62 Youido-Dong, Youngdeungpo-Gu, Seoul 150-713, South Korea
Received 21 May 2003; received in revised form 26 May 2003; accepted 27 May 2003

Abstract

The genetic variations for 15 short tandem repeat (STR) loci D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01,
D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA were performed on 231 unrelated Korean
population using commercially available AmpF/STR Identifiler kit.
# 2003 Elsevier Ireland Ltd. All rights reserved.

Keywords: Short tandem repeat; AmpF/STR Identifiler; Korean population

Population: Two hundred thirty-one unrelated indivi- 0.9999968, respectively. When we compared the data with
duals living in Korea. the value from other populations supplied by PE Applied
Extraction: Genomic DNA was extracted using the High Biosystems (Table 2). It showed that this AmpF/STR multi-
Pure PCR Template Preparation Kit (Roche, Mannheim, plex system in Korean has lower forensic capacity than
Germany). Caucasian and African. However, it has similar forensic
PCR: Multiplex PCR using 1–2 ng target DNA was capacity with Native American. Even though the TPOX
performed with the AmpF/STR Identifiler PCR Amplifica- locus showed significant deviation (P < 0:05) with lower
tion kit (PE Applied Biosystems, Foster City, CA, USA) PD, PIC and PE in a Korean population, 14 of 15 STR
according to the manufacturer’s instructions. loci except TPOX exhibited no significant deviation from
Typing: ABI Prism 310 Genetic Analyzer (PE Applied Hardy–Weinberg equilibrium. In recent years, the genetic
Biosystems). data for STR loci in a Korean population have been reported
Results: See Table 1. but they had a limited number of STR loci [1–5]. This is the
Quality control: Kit controls. first study of allele frequency distribution of D19S433 and
Analysis of data: The allele frequencies and statistical D2S1338 in a Korean population. More STR loci will be
analysis were performed on PowerStats program (http:// needed to successfully identify individuals in various situa-
www.promega.com/geneticidtools/Default.htm) and P value tions such as forensic identification, paternity testing, and
of exact test for Hardy–Weinberg equilibrium and expected engraftment assessment after hematopoietic stem cell trans-
heterozygosity were calculated by TFPGA (http://bioweb. plantation. In conclusion, we could obtain the potentially
usu.edu/mpmbio/index.htm) program. useful data using AmpF/STR Identifiler kit for individual
Other remarks: The combined PD and PE for the 15 identification in a Korean population and establish that
STR loci in a Korean population were 2:85  1017 and the use of AmpF/STR Identifiler PCR Amplification system
offers a highly polymorphic tool for genetic variation
*
Corresponding author. Tel.: þ82-2-3779-1001; analysis.
fax: þ82-2-785-1340. This paper follows the guidelines for publication of
E-mail address: dwkim@catholic.ac.kr (D.-W. Kim). population data requested by [6].

0379-0738/$ – see front matter # 2003 Elsevier Ireland Ltd. All rights reserved.
doi:10.1016/S0379-0738(03)00255-X
Table 1
Allele frequencies and statistical parameters for 15 STR loci in the Korean population

Allele D8S1179 D21S11 D7S820 CSF1PO D19S433 vWA TPOX D18S51 D3S1358 TH01 D13S317 D16S539 D2S1338 D5S818 FGA

6 17.3
7 0.2 0.4 24.7 0.2 1.3
8 11.9 47.8 0.2 3.7 22.3 1.1
9 0.2 3.7 4.8 12.8 48.9 16.5 30.1 11.5
9.3 5.4
10 15.2 15.2 24.2 3.7 0.2 14.1 17.7 17.7
11 8.4 40 23.6 0.2 32.9 1.5 23.6 27.3 29.4
11.2 0.2

Y.-L. Kim et al. / Forensic Science International 136 (2003) 92–95


12 18 21.9 38.1 2.8 2.6 5 0.2 19.9 17.1 25.3
12.2 0.4
13 21.9 6.5 7.4 32.7 0.2 18.4 2.2 7.6 12.1
13.2 2.6
14 16 0.6 0.9 26.8 20.6 25.1 3.5 1.3 1.5
14.2 10.2
15 14.1 0.6 5.4 2.2 18 35.1 0.2
15.2 15.2
16 4.5 18.2 8.7 33.1 0.6
16.2 3.2
17 1.7 30.3 6.7 18.4 10.2 0.4
17.2 0.2
18 18.2 4.5 8.9 14.7 3.5
19 8.9 5.4 0.9 18.2 6.9
19.2 0.2
20 1.7 2.8 11.3 4.5
20.2 0.2
21 2.6 1.9 14.1
22 0.4 2.8 16.9
23 0.4 17.5 22.5
23.2 0.2
24 13.4 18
25 6.9 9.5
25.2 0.2
26 1.5 3
27 0.4
28 3.7
28.2 0.9
29 25.1 0.2
30 37.4
30.2 0.4
31 9.7

93
31.2 7.1
94
Table 1 (Continued )

Allele D8S1179 D21S11 D7S820 CSF1PO D19S433 vWA TPOX D18S51 D3S1358 TH01 D13S317 D16S539 D2S1338 D5S818 FGA

Y.-L. Kim et al. / Forensic Science International 136 (2003) 92–95


32 2.4
32.2 7.6
33 0.6
33.2 4.1
34 0.2
34.2 0.4
35 0.2
Mpa 0.046 0.083 0.097 0.114 0.084 0.075 0.182 0.043 0.123 0.164 0.066 0.091 0.035 0.077 0.042
PDb 0.954 0.917 0.903 0.886 0.916 0.925 0.818 0.957 0.877 0.836 0.934 0.909 0.965 0.923 0.958
PICc 0.82 0.74 0.71 0.69 0.75 0.76 0.58 0.83 0.68 0.62 0.78 0.73 0.85 0.76 0.83
PEd 0.675 0.641 0.472 0.486 0.577 0.561 0.298 0.744 0.424 0.385 0.577 0.523 0.7 0.585 0.658
TPIe 3.12 2.82 1.83 1.89 2.36 2.26 1.27 3.98 1.65 1.52 2.36 2.06 3.4 2.41 2.96
Hobsf 84 82.3 72.7 73.6 78.8 77.9 60.6 87.4 69.7 67.1 78.8 75.8 85.3 79.2 83.1
Hexpg 84 77.5 75.1 73.4 78.4 79.3 64.6 85.2 72.6 66.9 80.9 77.1 86.9 79.1 85.4
Ph 0.959 0.623 0.802 0.848 0.658 0.547 0.032 0.661 0.059 0.204 0.600 0.963 0.572 0.908 0.345
a
Matching probability (MP).
b
Power of discrimination (PD).
c
Polymorphism information content (PIC).
d
Power of exclusion (PE).
e
Typical paternity index (TPI).
f
Observed heterozygosity (Hobs).
g
Expected heterozygosity (Hexp).
h
Exact test for HWE (P).
Y.-L. Kim et al. / Forensic Science International 136 (2003) 92–95 95

Table 2
Comparison of the probability of identity and paternity exclusion values for the 15 STR loci in the various ethnic populations

Locus African-American US Caucasian (PD/PE) US Hispanic Native American This study (Korean)

CSF1PO 0.079/0.545 0.132/0.496 0.141/0.450 0.123/0.409 0.108/0.675


D2S1338 0.023/0.748 0.027/0.725 0.038/0.671 0.043/0.399 0.036/0.641
D3S1358 0.097/0.591 0.076/0.630 0.112/0.495 0.158/0.510 0.134/0.472
D5S818 0.104/0.506 0.147/0.440 0.115/0.525 0.110/0.601 0.082/0.486
D7S820 0.085/0.591 0.063/0.582 0.083/0.574 0.081/0.492 0.108/0.577
D8S1179 0.074/0.580 0.064/0.680 0.089/0.599 0.104/0.601 0.048/0.561
D13S317 0.132/0.383 0.079/0.487 0.056/0.638 0.056/0.370 0.069/0.298
D16S539 0.077/0.649 0.097/0.566 0.090/0.567 0.082/0.428 0.091/0.744
D18S51 0.033/0.760 0.031/0.731 0.031/0.767 0.046/0.329 0.041/0.424
D19S433 0.042/0.601 0.087/0.531 0.049/0.678 0.044/0.360 0.086/0.385
D21S11 0.037/0.737 0.044/0.708 0.047/0.586 0.074/0.399 0.076/0.577
FGA 0.034/0.760 0.035/0.766 0.032/0.739 0.031/0.309 0.043/0.523
TH01 0.109/0.492 0.079/0.566 0.097/0.618 0.134/0.646 0.163/0.700
TPOX 0.089/0.521 0.188/0.329 0.168/0.392 0.159/0.687 0.189/0.585
vWA 0.066/0.709 0.066/0.625 0.080/0.555 0.103/0.528 0.079/0.658
Combined 1.31  1018/0.9999996 5.01  1018/0.9999992 7.65  1018/0.9999990 3.62  1017/0.999952 2.85  1017/0.9999968

Acknowledgements STR loci FGA, vWA, D3S1358, D18S51, D21S11, D8S1179,


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