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Black Crappie Sarcoma

Background:

Black crappies from the Madison Lakes presented with large open raised sores as early as the late 1980’s.
Originally this condition was diagnosed via electron microscopy as possibly being related to a rickettsial agent,
although never confirmed. At the same time fish from Staples Lake (Barron and Polk Co.) were noticed to have
the same condition. In the mid-2000s the condition started being reported in large numbers in the Polk, Barron,
Dane counties and the Lake Winnebago area. Incidence appears to run in cycles with the condition going from 1-
2% to as high as 20-30% of fish being affected. Currently the Polk and Barron regions seem to be the most
heavily affected. Actual prevalence is not known; however, on some lakes anglers have begun to voluntary
monitor and keep records of the prevalence of infected fish. In 2005/6 an unknown virus was isolated from 1
sample and sent to Dr. Tom Waltzek for identification. However, due to unfortunate circumstances the virus was
damaged in the shipping and could not be analyzed. In addition, it has never been isolated from any subsequent
sampling.

Clinical signs:

Lesions can appear anywhere on the body of black crappies; however, they are often located along the
operculum, mid-body, dorsal, anal, and pelvic fins. They develop into large open raised sores that penetrate
deep into the muscle eventually resulting in liquefactive necrosis of tissue.

Preliminary Diagnosis:

Round cell sarcoma


Unknowns:

- Cause of sarcoma –virus, rickettsia, bacteria, other


- How is it transmitted?
- Are infected fish safe to eat??
- Currently seems to only affect the larger fish but are the younger fish affected and not showing signs? Is
this a concern?
- Fate of fish
- Is the lesion more likely to be displayed in cooler water temperatures?
- Are there any environmental/lake specific conditions limiting the spread of BLC sarcoma? Black crappies
have a wide distribution, but BLC sarcoma occurs in relatively few places?

Plans:

Collected new samples for:

- Viral cell culture


- Next Generation sequencing – Dr. Tony Goldberg’s lab
- Blood films – rickettsia
- Histopathology
- Bacteriology
- Electron Microscopy of lesion – virus vs. rickettsia, viral identification

Financial Needs:

- Next Generation Sequencing


- Electron Microscopy
- Molecular Testing

Feb 16, 2015


MF

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