mAU (a) (b)

mAU
DAD1 B, Sig=292,8 DAD1 B, Sig=292,8
350 400
Ref=off (VAV0613\007-0901.D) Ref=off (VAV0613\006-0801.D)
350
300

300
250
250

2.769
200

2.762
200
150
150

100
100
3.129
3.449

3.125
3.442
50
7.866
7.159

50

7.851

10.70
8
0 0

0 5 10 min 0 5 10 min
 RT: 0.00-17.12 SM: 7G

8.65
RT: 0.00-17.16 SM: 7G

8.03
100 NL: 100 NL:
(a) 3.64E7

8.63
(b) 3.31E7
90 TIC MS 90 TIC MS
2008-620
2008-619
80 80

70 70
Relative abundance

Relative abundance
60 60

50 50

40 40

5.58
5.59

30 30

20 20

14.48
10.47

10.46
11.69
13.08

16.22

13.07

15.71
16.69
11.94 10

14.50
10

9.51
9.52
3.86

6.62

3.71

6.61
4.78
2.92

4.79

0 0
0 2 4 6 8 10 12 14 16 0 2 4 6 8 10 12 14 16
Time (min) Time (min)
 Norm.

10.0
12.5
15.0
17.5

-5.0
-2.5
0.0
2.5
5.0
7.5
Norm.
0
5
10
15
20
25

-5

0
0
(a)

(b)
2.293-asp 2.292-asp
2.597-glu 2.587-glu

4.481

5
5
5.357-ser 5.358-ser
6.148-his 6.145-his
6.920-gly 6.916-gly
7.345-thr 7.332-thr

8.821-arg 8.818-arg
9.609-ala 9.604-ala

10
10

12.091-tyr 12.089-tyr
WWD1 A, wavelength = 338nm, TT (20080603\060330.D)

WWD1 A, wavelength = 338 nm, TT (20080603\060331.D)

15
15

15.397-cys-s 15.394-cys-s
16.983-val 15.977-val
16.358-met 16.354-met

18.129-trp 18.132-trp
19.026-phe 19.032-[he
19.458-lie 19.464-ile

20
20

20.758-lue 20.769-leu

21.964-lys 21.979-lys

25
25

28.152-pre 28.164-pro
min
min
 Transmittance (%) Transmittance (%)

0
10
20
30
40
50
60
0
10
20
30
40
50

4000
70 (b)
(a)

3336.22 3340.26
2957.26

3000
2925.92
2926.97

2000
Wave no. (G 1)
1655.39 1651.37
1535.40 1536.27
1401.06 1450.94
1315.94
1237.66

1075.55 1071.97
1000
780.59