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PSY 106

Chapter 5 Worksheet

Name ______________________________________________ Score ______________


Technique Information Provided Advantages Disadvantages
Permanent lesions Behavior no longer Connect brain function May misinterpret
produced infers to behavior behavioral information
function of location of All areas of brain
lesion. interconnected

Aspiration General area involved Surface lesion Unspecific damage


in functions
Radio-frequency lesion General area involved Internal lesions Unspecific damage
in functions Damages all cells and
paths in the area

Excitototoxic Specific area involved Selective damage Unspecific damage—


(kainic acid—amino in functions Destroys only cell stimulates many types
acid) bodies—not pathways of neurons
Kills neurons that it
effects when high dose
used
6-hydroxy dopamine Destroys only More specific Kills neurons that it
adrenonergic and Only neurons using effects
noradrenergic neurons specific
neurotransmitters
Temporary lesions Inhibition of Not limited to animal May not be specific
functioning and research—medical enough
behavior is reversible applications
Anesthetic Blocks action potentials Reversible
Inhibition (Muscimol) Activates GABA Reversible
receptors—inhibits
function of neuron in
area

Stimulation Increases activity in a Can determine the


region or particular effect of increased
types of cells activity in cells

Electrodes Damage other cells


with placement
Drugs/ Can target specific
Neurotransmitters cells
Histology
Destroy autolytic Reduces or prevents
enzymes deterioration of tissue
PSY 106
Chapter 5 Worksheet

Name ______________________________________________ Score ______________


Perfusion Removes blood cells
from tissue
Preserve (Fixative) Stabilizes tissue Prevents deterioration
or distortion of cells in
tissue

Section (Microtome) Makes tissue thin


enough to examine
using microscopy
Stain
Methelyne blue or Color cell bodies All cell bodies stained Separate in image
cresyl violet and visible relative to
other elements in tissue
Microscopy

Electron microscopy

Confocal laser
scanning microscopy
Anterograde labeling
methods

Immunocytochemical Lectins fill cells


methods—dyed Afferent Connections
sntibodies

Retrograde labeling
methods
Fluorogold Taken up by terminal
buttons and transported
to cell bodies

Transneuronal
PSY 106
Chapter 5 Worksheet

Name ______________________________________________ Score ______________

Recording neuronal
activity
Microelectrodes Single unit recording—
one neuron

Macroelectrodes Brain region

Electroencephalogram Detect epilepsy or


monitor procedures
Magnetoencephalograp
hy
Recording the brain's
metabolic and synaptic
activity

Autoradiography 2-deoxyglucose (2-


DG)—taken into most
active cells
Positron Emission Radioactive 2-DG
Tomography (PET) activity of regions
Functional Magnetic Detect levels of oxygen
Resonance Imaging in blood vessels
(fMRI)

Measuring the brain’s


secretions

Microdialysis Tube collects


extracellular fluid
Detect levels of
neurotransmitter
Stimulating neural
activity
Electrical current

Chemicals (Cannula) Localized, effects just


cell bodies
Microiontophoresis Small amounts of
neurotransmitter
PSY 106
Chapter 5 Worksheet

Name ______________________________________________ Score ______________


Transcranial magnetic Magnetic fields
stimulation interferes with
functioning of location
in brain

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