Académique Documents
Professionnel Documents
Culture Documents
ABSTRACT
The human body uses proteins for many things, including repairing and building tissues,
acting as enzymes, aiding the immune system, and serving as hormones. Each of these important
functions requires a slightly different form of protein. In spite of their differences in structure, all
Earlier, we mentioned that protein plays a role in tissue repair, and that's why it's so
important to have protein in your diet. But what are the best sources of protein? Afterwards, you
might take a look in your refrigerator and decide whether your diet is protein rich or protein
poor.
OBJECTIVES
samples.
Pipette
Biuret reagent
Spectrophometer
Protein samples (Pony Fish “ikan kekek” & Torpedo Scad “ikan cencaru”)
PROCEDURES
1. We prepared the biuret solution, by adding, 300ml of 10% (w/v) NaOH to 500ml of a
solution containing 0.3% copper sulphate pentahydrate and 1.2 sodium potassium
b. Protein preparation
1. First we labelled the test tube using sticker paper to make easier for us to record the
result
3. Then we also pipetted the different amount of water in each tube to make sure the
concentration diluted.
1 0 1 2ml
15 minutes incubation
4 0.3 0.7 2ml
1. 1ml of solution from tube 1 was transferred into a cuvette and wiped the cuvette with
3. The absorbance of the other standards and sample proteins were measured using the
step as in (1).
1 0.000 0
2 0.036 1
3 0.085 2
4 0.088 3
5 0.122 4
6 0.291 5
7 0.297 6
prepared. We used gelatine as our source of the protein but with different concentration in each
tube. Gelatine is yellowish, odourless and nearly tasteless substances that are made by prolonged
boiling of skin, cartilage, and bones from animals. It is a mixture of peptides and proteins
produced by partial hydrolysis of collagen extracted from skin, bones, and connective tissues of
From tube 1 to tube 7, each tube were added 0.1 ml until 0.6 ml of gelatine in each tube.
Tube 1 is a negative control which contains no gelatine at all with 1 ml and 2ml of biuret in it. As
the result, the absorbance shows 0.00. The absorbance increased proportionally with the
increasing of the protein concentration in each tube. Test tube 7 shows the highest absorbance
Me and my laboratory partner decided to bring our laboratory samples that is Torpedo
Ponyfishes are small and laterally compressed in shape, with a bland, silvery colouration.
They are distinguished by highly extensible mouths, and the presence of a mechanism for
locking the spines in the dorsal and anal fins. We would like to determine which fish have the
highest portion of protein. First of all, we separated the bones from the fish and blended their
fillet to make sure that it is easier for us to extract their protein. After that, for about 0.2 g of fillet
were separated and were crushed with mortar and pestle. Then, diluted with water for about 10ml
to 12 ml depends on how the concentration look. If it too concentrated, then add more.
The solution of the fillet then undergoing a process which is to separate the fluid from the
fillet . We only want the protein extracted from the fillet in a liquid form. Vortex and centrifuge
it for about few minutes to get the protein extraction. When the liquid is ready proceed the
procedures stated.
We does not know the protein concentration from the protein extraction. But we do have
their absorbance value. So , we can plot a graph of absorbance versus protein concentration of
the 7 tubes prepared before using gelatine. The absorbance for pony fish and torpedo scad
protein must be in between range from test tube 1 to test tube 7. So we get the value 4.2 for the
torpedo scad which contains higher protein concentration rather than pony fish protein with the
value of 1.3.
REFERENCES
1. Nutrition source
https://www.hsph.harvard.edu/nutritionsource/what-should-you-eat/protein/
https://www.atascientific.com.au/3-protein-analysis-techniques/
LEARNING REFLECTION
It is actually new thing for most of my classmates as it is our very first time using and
experiencing to use spectrophotometer. Our lecturer Dr. Shakinaz brief to us about how to use
and how spectrophotometer actually works. Learn from mistakes is a must. In order to be good in
laboratory, we must apply those step and if we do make some mistake, learn from it. I do. We all
do actually. The spectrophotometer cannot read our protein sample as the sample is too
concentrated. We need to dilute the protein sample so that the spectrophotometer can read ours.
During our laboratory session, at first the spectrophotometer cannot read our protein sample.
After another preparation of protein sample with a different dilution techniques, we manage to
record it reading.