Académique Documents
Professionnel Documents
Culture Documents
Course Objec6ves
Instructor: Dr. Aparna Zama
Email: zama@sebs.rutgers.edu • Develop a thorough understanding of the
mammalian endocrine system
Office: BartleH, Room 209A
• Cri5cally examine the role of hormones in the
Tel: 848-932-8495 physiology of the organism
Office hours: by appointment, arrange via email • Study the mechanisms of hormone ac5on
Graduate Teaching Assistant: Mariana Saboya • Develop an understanding of endocrine
pathologies
Email: mariana.saboya@rutgers.edu
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Sakai
• Lectures will be posted in “Resources”
folder in hPps://sakai.rutgers.edu.
• Lectures highlight the important aspects of
endocrinology as referenced in the
textbook.
• For success in this course: read relevant
chapter/s, follow lectures carefully, take
detailed notes in class, and revisit
textbook.
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Exams
• 3 exams
• Final exam
Date: Apr 27 (during class period)
Grading
Two mid-term exams (25% each) 50%
• Sanc5ons for separable viola5ons include, but are not limited to, one or more of
the following, and may, but need not, involve suspension or expulsion:
• A grade of XF (disciplinary F) for the course.
One comprehensive final exam 25%
• Disciplinary proba5on.
• Dismissal from a departmental or school honors program. Quizzes and assignments 25%
• Denial of access to internships or research programs.
100%
• Loss of appointment to academically-based posi5ons.
• Loss of departmental/graduate program endorsements for internal and external
fellowship support and employment opportuni5es.
• Removal of fellowship or assistantship support.
• Suspension for one or more semesters. Fill in the blanks, multiple choices, true/false, short answers, and essays
• Dismissal from a graduate or professional program.
• Permanent expulsion from the University with a permanent nota5on of disciplinary
expulsion on the student’s transcript.
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Syllabus
Outline for introductory lectures
• Hormones and the endocrine system
• The classifica5on of hormones
• The biosynthesis, storage, and control
of hormone release
• Circula5on and metabolism
• Hormone – receptor interac5ons and
downstream signaling
• Homeosta5c control
• Methods used in endocrinology
Hormones
• Cell-to-cell communica5on molecules
– Chemical signals, released in small amounts • Growth factors act at short distances
– Secreted by a cell or group of cells into the blood – Ac5vates physiological response at low
– Transported by blood concentra5ons
– Distant target 5ssue receptors
– Cellular mechanism of ac5on
• Ectohormones are released into the environment
• Depends on binding to target cell receptors • Pheromones: Elicit physiological or behavioral
• Ini5ates biochemical responses response on other organisms of the same species
– Hormone ac5on must be terminated
• Half-life indicates length of ac5vity
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• Levels of organiza5on
• Structure – Func5on
– Cell communica5on is cri5cal
• Homeosta5c regula5on
– Internal environment has to be maintained
constant within narrow limits, no maHer what
condi5ons prevail in the external environment
– Feed back mechanisms
The endocrine system, nervous system, and immune system each secretes its own bioregulators: hormones,
neurocrines, and cytocrines, respectively. However, all of these systems influence each other, and from a
homeostatic viewpoint we can assume they function as one great bioregulatory system. 20
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FIGURE 1.1
21 22
Chemical communica6on between cells, an Levels at which hormone ac6ons are considered
addi6onal view
24
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excretion
A hormone is “born” in an endocrine cell and spends its short life “free” in the blood or bound to binding proteins. It may be
metabolized and/or excreted (“die”) before or after it binds to a target cell where it causes changes that result in its characteristic
effect. In some cases, the hormone is secreted in an inactive form and must be metabolized to an active form before it can 25 bind
to its receptor and produce an effect.
Goals and
Objectives
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Hormone biosynthesis
and release
Basic molecular
biology of the cell
as related to
hormone
biosynthesis
and release
(A) Cords of cells secreting growth hormone (orange) and gonadotropins (blue) in a pituitary gland. (B) Islet of insulin secreting cells
(arrow) embedded within the darker stained exocrine pancreas. (C) A collection of follicles (consisting of an epithelium surrounding a
fluid-filled lumen) from a thyroid gland showing a thin epithelium and pink colloid (a protein suspension) filling the lumen of the 31
follicle. (D) Isolated clusters of testosteronesecreting interstitial cells (arrow) located between seminiferous tubules in a testis.
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FIGURE 1.5
FIGURE 1.3
DNA is a polymer of the five-carbon sugar, deoxyribose, in diester linkage with phosphate forming ester
bonds with hydroxyl groups on carbons 3 and 5 on adjacent sugar molecules. The purine and pyrimidine
bases are linked to carbon 1 of each sugar. The numbering system for the five carbons of deoxyribose are
shown at the top of the figure. The chemical bonds forming the backbone of the DNA chain are highlighted
in blue. The 5’ or 3’ ends refer to the carbons in deoxyribose.
33 34
Alternative Splicing
Translation
FIGURE 1.7
FIGURE 1.6
35 36
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Post-transla6onal processing
FIGURE 1.8
The leader sequence or signal pep*de of proteins des5ned for secre5on enters the cisternae of the endoplasmic re5culum
even as pep5de elonga5on con5nues. In the endoplasmic re5culum (1) the leader sequence is removed, (2) the protein is
folded with the assistance of protein chaperons, (3) sulgydryl bridges may form, and (4) carbohydrate may be added
(glycosyla5on). The par5ally processed protein (5) is then entrapped in vesicles that bud off the endoplasmic re5culum and
(6) fuse with the Golgi apparatus, where glycosyla5on is completed, and (7) the protein is packaged for export in secretory
37
vesicles in which the final stages of processing take place.
Slide 1
The prohormone
passes from the ER
through the Golgi
complex.
Golgi
complex
Secretory vesicles
containing enzymes
and prohormone bud
off the Golgi. The
enzymes chop the
prohormone into one
or more ac6ve
pep6des plus
addi6onal pep6de
Secretory fragments.
vesicle Ac6ve hormone
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Pep6de
Hormone
Synthesis
and
Processing
FIGURE 1.9
Exocytosis. 1. Immature secretory vesicles bud off the trans-Golgi stacks. 2. Matura5on of the vesicle
includes extrusion of some proteins and water, acidifica5on of vesicle contents, and condensa5on of
enclosed proteins to form dense core granules. 3. Mature vesicles residing deep in the cytosol as a reserve
pool await a signal for recruitment (4) to the readily releasable pool adjacent to the plasma membrane. 5. In
prepara5on for secre5on, the vesicles become tethered to the membrane (docking). 6. An energy-
dependent interac5on forms a loose associa5on of special proteins (SNARE proteins) in the membranes of
the vesicles with counterparts in the plasma membrane, “priming” the vesicles to respond to a secretory
s5mulus. 7. Secre5on is triggered by an increase in cytoplasmic calcium that produces conforma5onal
changes in the SNARE proteins that brings the membranes of the vesicles into such close apposi5on to the
plasma membrane that fusion occurs and a secretory pore is formed. 8. Expansion of the pore as the vesicle
membrane is incorporated into the plasma membrane releases vesicular contents into the extracellular
fluid.
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Amine
is the parent amino acid for
catecholamines and thyroid hormones.
or Amine
hormones
• Amine hormones: Tryptophan - melatonin Catecholamines Thyroid hormones
are synthesized from two
are made by modifying
• Thyroid hormones
Epinephrine Triiodothyronine (T3)
Blood
Cell Cell
Endocrine without with
cell receptor receptor Target
cell
No response
FIGURE 1.10
Response
Hormone binding to plasma proteins. Bound hormone is in equilibrium with a small frac5on of “free” unbound hormone.
51
Only the free hormone can pass through capillary endothelium to reach target cells or sites of degrada5on.
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FIGURE 1.11
Although all cells come in contact with the hormone, only the cells colored blue have receptors and therefore can respond
to the hormone. (H = hormone; HR = hormone receptor) 53
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