ABSTRACT

The purpose of this experiment to find a decisive manner of levels of sulphate in a

water sample river water near biology ITS and X sample by using the method
spektrofotometri sulphate and know whether water sample in accordance with of
quality standard clean water.
The method to this experiment at first is calibration. How which is to build
blanko solution then adjusted wavelengths of 420 nm, absorbance 0 and transmittance
100 . Second, the analysis sulphate. How which is to build standard solution with
concentration of the 150 ppm then diluting with aquades 250 ml. To make
concentration 120 ppm, 90 ppm, 60 ppm and 30 ppm by means of dilution. Then,
continued make a solution (a).
Conclusion on standard solution H 2SO4 obtained concentration 150 ppm , 120
ppm , 90 ppm , 60 ppm , 30 ppm with absorbance each of i; j; k; l; m. The Concentration
water sample of biology’s ITS river is x ppm and X sample is y ppm . Both of results is too
far from standard clean water about 400 ppm for clean water and drinking water about
250 ppm .

Keywords : spectrophotometer, standard solution, sulphate solution

1
 ABSTRAK

Tujuan percobaan ini adalah untuk mengetahui cara menentukan kadar sulfat
didalam sampel dengan menggunakan metode spektrofotometri sulfat dan mengetahui
apakah sampel air sungai dekat biologi ITS dan sample X sesuai dengan baku mutu air
bersih.
Metode untuk percobaan ini yaitu pertama kalibrasi alat. Caranya membuat
larutan blanko kemudian mengatur panjang gelombang 420 nm, absorbansi 0 dan
transmittan 100. Kedua, tahap analisa sulfat. Caranya membuat larutan standard
konsentrasi 150 ppm kemudian mengencerkan dengan aquades 250 ml. Untuk
membuat konsentrasi 120 ppm, 90 ppm, 60 ppm dan 30 ppm dengan cara pengenceran.
Dilanjutkan membuat larutan (a).
Kesimpulan pada larutan standard Na2SO4 didapat konsentrasi 150 ppm, 120
ppm, 90 ppm, 60 ppm, 30 ppm dengan absorbansi i; j; k; l; m. Konsentrasi sampel air
sungai dekat biologi ITS x ppm dan sampel X sebesar y ppm. Kedua hasil ini jauh dari
standard air bersih yang takarannya sebesar 400 ppm untuk air bersih dan air minum
sebesar 250 ppm.

Kata Kunci : spektrofotometer, larutan sulfat, larutan standard

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 DAFTAR ISI

ABSTRACK .......................................................................................................................................... i
ABSTRAK............................................................................................................................................ ii
DAFTAR ISI....................................................................................................................................... iii
DAFTAR GAMBAR.......................................................................................................................... iv
DAFTAR TABEL................................................................................................................................ v
BAB I PENDAHULUAN
I.1 Latar Belakang ............................................................................................................. I-1
I.2 Rumusan Masalah ...................................................................................................... I-1
I.3 Tujuan Percobaan ....................................................................................................... I-1
BAB II TINJAUAN PUSTAKA
II.1 Dasar Teori ................................................................................................................. II-1
II.1.1 Pengertian Spektrofotometri........................................................................... II-1
II.1.2 Jenis – Jenis Spektrofotometri.........................................................................II-1
II.1.3 Prinsip Metode Spektrofotometris................................................................II-2
II.1.4 Cara Kerja Spektrofotometer...........................................................................II-3
II.1.5 Kalibrasi.................................................................................................................... II-4
II.2 Baku Mutu Air Bersih dan Air Minum..............................................................II-4
II.3 MSDS Natrium Sulfat (Na2SO4)............................................................................ II-7
II.4 Aplikasi Industri ...................................................................................................... II-10
BAB III METODOLOGI PERCOBAAN
III.1 Variabel Percobaan .............................................................................................. III-1
III.2 Alat Percobaan ...................................................................................................... III-1
III.3 Bahan Percobaan .................................................................................................. III-1
III.4 Prosedur Percobaan ............................................................................................ III-1
III.5 Diagram Alir ........................................................................................................... III-2
III.6 Gambar Alat Percobaan ..................................................................................... III-3
DAFTAR PUSTAKA ........................................................................................................................ vi
DAFTAR NOTASI ........................................................................................................................... vii
APENDIKS ...................................................................................................................................... viii
LAMPIRAN
1. Jurnal
2. Laporan Sementara
3. Lembar Revisi

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 DAFTAR GAMBAR

Gambar II.1.1 Skema Sebuah Spektrofotometri Single Beam..................................II-1

Gambar II.1.2 Absorbansi Sinar Cahaya oleh Larutan Pewarna.............................II-2
Gambar II.1.3 Spektrum GEM............................................................................................... II-2
Gambar II.1.4 Cara Kerja Spektrofotometer...................................................................II-3
DAFTAR TABEL

Tabel II.1.1 Parameter Wajib (Air Minum)......................................................................II-4

Tabel II.1.2 Parameter Wajib (Air Bersih).......................................................................II-6
Tabel II.1.3 MSDS Natrium Sulfat (Na2SO4).....................................................................II-7

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 DAFTAR PUSTAKA

Alaerts .G & Sri Sumestri Santika. 1984.Metode Penelitian Air.Surabaya-Indonesia:

Usaha Nasional
Day. & A.L.Underwood. 2002.Analisa Kimia Kuantitatif. Jakarta: Erlangga
Lestari Fatma. 2007.Bahaya Kimia Sampling & Pengukuran Kontaminan di udara.
:EGC
Taslimah,dkk.2003.”Pemerangkapan Garam Ammonium Sulfat Dalam
Zeolit”.Universitas Diponegoro: Kimia,FMIPA
Peraturan Menteri kesehatan Republik Indonesia No.416 Tahun 1990: Tentang
Syarat-syarat Dan Pengawasan Kualitas Air
Peraturan Menteri kesehatan Republik Indonesia Nomor 492/Menkes/Per/IV/2010:
Tentang Persyaratan Kualitas Air Minum
Luthfi, A. Kalibrasi Spektrofotometer. Retrieved from dokumen.tips:
http://dokumen.tips/documents/kalibrasi-spektrofotometer.html
Seran, E. Prisip Kerja Spektrofotometer. Retrieved from wordpress.com:
https://wanibesak.wordpress.com/tag/prinsip-kerja-spektrofotometer/

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 DAFTAR NOTASI

Notasi Nama Notasi Satuan

N Normalitas grek/liter larutan
V Volume Mililiter atau liter
M Molaritas Mol/liter
m Massa Gram
ppm Part per milion Ppm
BM Berat Molekul Gram/Mr
ρ Densitas Gram/L
e Ekuivalen -

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 APENDIKS

 Membuat larutan Standar Na2SO4

Diketahui:
1 ppm = 1 mg/l
a. Larutan Na2SO4 1000 ppm
1000 mg
1000 ppm =
1000 ml
= 1000 mg/mL
= 1 g/L
b. Larutan Na2SO4 150 ppm
N1 . V1 = N2 . V2
1000. V1 = 150 . 100 mL
15000
V1 =
1000
V1 = 15 mL + 85 mL aquades
c. Larutan Na2SO4 120 ppm
N1 . V1 = N2 . V2
150. V1 = 120 . 100 mL
12000
V1 =
150
V1 = 80 mL + 20 mL aquades
d. Larutan Na2SO4 90 ppm
N1 . V1 = N2 . V2
120. V1 = 90 . 100 mL
9000
V1 =
120
V1 = 75 mL + 25 mL aquades
e. Larutan Na2SO4 60 ppm
N1 . V1 = N2 . V2
90. V1 = 60 . 100 mL
6000
V1 =
90
V1 = 66,6 mL + 55,4 mL aquades
f. Larutan Na2SO4 30 ppm
N1 . V1 = N2 . V2
60. V1 = 30 . 100 mL
3000
V1 =
60
V1 = 50 mL + 50 mL aquades