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What is a plasmid? small, extracellular, generally circular DNA molecules, that are typically 2-
100kb in size and carry non-essential genes that may offer a select advantage to the bacteria
What is an episome? Plasmid that can replicated automostly as well as integrated inti
bacterial chromosome.
4. specialized transduction Phage: Transfers genes from a particular region of the bacterial
chromosome.
5.generalized transduction Phage: Transfer DNA derived from any part of the bacterial
chromosome.
6.What is a F factor: (The Fertility factor) allows genes to be transferred from one bacterium
carrying the factor to another bacterium lacking the factor by conjugation.
* The F factor is an episome: A genetic element that can insert into chromosome or replicate as
Cingular plasmid
7.Hfr strain: High frequency of recombination. F factor is integrated into the bacterial
chromosome, behave as F+ cells forming sex pili and undergoing conjugation with F- cells, only
allows recombination usually doesn't result in F+
8.F’ factor: F factor has excised from the bacterial chromosome and taken a small amount of the
bacterial chromosome with it, these bacterial chromosomal genes are carried with the F plasmid
9. auxotroph: Mutant organisms requiring certain nutrients for growth. They are unable to
survive in minimal medium
10. prototroph? Naturally self-sufficient , they can exist on a subtracted of simple inorganic
salts and minimal culture medium.
11. Is an F+ cell a donor or recipient in the process of conjugation?
The donor cell is F+; carries the fertility factor. Conjugation (mating) occurs between an
F+ donor and an F- recipient
12. Can conjugation occur between two F+ cells? Can it occur between two F- cells?
14. What is the relationship between frequency of co-transformation and the distance
(linkage) between two genes?
genes that are closely linked will have a higher chance of co-transformation; if co-transformation
for two genes is higher than the product of the two individual transformation freq. then the two
genes must be close together
Transduction: a process by which bacteriophages (bacterial viruses) transfer genes from one
bacterium to another, once the DNA has been introduces into a recipient cell it can undergo
genetic recombination with a homologous sequences in the host (recipient) chromosome
Lysogenic Cycle
Step one?
bacteriophage inserts its genome into the host bacterial chromosome. literally becomes part of
host chromosome
step two?
prophage will remain integrated and replicate along with the host chromosome until
environmental conditions become bad
These cells have a choice of their life cycle: lytic or lysogenice they are called?
Temperate phages
specialized transduction?
temperate phages are capable of this, only specific genes can be transferred by this mechanism.
Specifically, the genes that flank the integration site