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Development and validation of analytical method for

the estimation of lamivudine in rabbit plasma

→ We plot the standard calibration curve of lamivudine in rabbit plasma in excel:


And find out: Linearity range (ng/mL): 25-2000

Linear regression equation: Y= 0.0011X + 0.0104

Correlation coefficient (reproducibility): R2= 0.9987

Slope (calibration sensitivity): m= 0.0011

Intercept: b= 0.0104

ng
Mean( )∗100%
mL
→ Accuracy= ng
Concentration( )
mL

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