Soportes Presentaciones

(744f) Dynamic Treatment Strategies
for Methanol Intoxication Using

Ethanol As Alcohol Dehydrogenase
Inhibitor in Human Beings
 Conference: AIChE Annual Meeting

 Year: 2012
 Proceeding: 2012 AIChE Annual Meeting
 Group: Computing and Systems Technology Division
 Session:
Control In Medicine and Biology
 Time:
Thursday, November 1, 2012 - 5:00pm-5:20pm
Authors:
Vargas, R. D., Universidad de los Andes
Galvis, A. A., Universidad de los Andes
Moreno, J., Universidad de los Andes
Vargas, W. L., Universidad de los Andes
Methanol poisoning is a fatal public health problem that affects population in both
developed and developing countries particularly as alcohol consumption increases in most
countries around the world. Several sources of poisoning are related to this issue (e.g.
adulterated beverages, inappropriate distillation processes, methylated spirits, accidental
exposure to this organic solvent, suicide attempt cases, among others). The inlet routes to
the systemic metabolism are usually ingestion, inhalation or absorption through the skin.
Several isolated cases of mass intoxications have taken place worldwide in recent years,
Nicaragua (788 cases, 2006); Colombia (31 cases, 1989 and 88 cases, 2004); Jordan (17
cases 2006), Estonia (154 cases, 2001) [1-6]. Nevertheless, the total of cases per year in
each country might be higher: Colombia (136 cases, 2007 and 250 cases, 2008); United
States (2283 possible cases, 2007) [7].
Methanol toxicity arises due to two main accepted mechanisms: i) central nervous system
depression and ii) biotransformation of methanol into formaldehyde and then to formic
acid. Therefore, current treatments approaches are related to alcohol dehydrogenase (ADH)
inhibition, since this enzyme is the one responsible for methanol metabolism to
formaldehyde (the toxic metabolite). In emergency settings and with the purpose of treating
patients for Methanol poisoning, inhibition is carried out using intravenous injection of
“pure” ethanol or 4-methylpirazole (fomepizole) because these compounds may compete
for the active sites on the ADH enzyme.
Several in-silico models for alcohols and glycols have been developed and the
physiologically based pharmacokinetic (PBPK) ones are the most common because these
enhance the fitting with experimental data keeping the physiological meaning of each
differential equation [7-8]. Nevertheless, in the state of art there are few models that include
non-reactive elimination pathways or dynamic control strategies in order to provide the
most accurate doses for the inhibition kinetics of ADH in a physiological media.
In this study a multi-compartment physiologically based pharmacokinetic model including
non-reactive elimination pathways, and a dynamic optimization technique is implemented
in order to minimize the formaldehyde production on the alcohol dehydrogenase ADH
enzyme using intra-venous ethanol as inhibitor. This technique is contrasted with a PID
control strategy designed to stabilize the vein formaldehyde concentration under critical
levels, the controller parameters are tuned up via nonlinear optimization techniques.
The proposed PBPK model includes separation between venous and arterial blood pools,
and the main organs for methanol, ethanol and its metabolites biodistribution have been
modeled in detail (i.e. Liver, lungs, kidneys, muscles and fat, stomach, gastrointestinal tract
and skin), and the reactive unit (liver) is modeled as series of CSTR reactors with a
reversible Michaelis-Menten kinetics. Additionally, the parameters for this model have
been adjusted and validated using experimental data from the literature.
Keywords: Methanol intoxication, enzymatic inhibition, dynamic optimization, PID

control.
References:
[1] Pan American Health Organization, Methanol Poisoning in Leon, Nicaragua, Report,
September, (2006).
[2] Bennett I.L., Cary F.H., Mitchell G.L., et al., Acute methyl alcohol poisoning: A review
based on experiences in an outbreak of 323 cases. Medicine 32:431-463, (1953).
[3] Kane R.L., Talbert W., Harlan J., et al., A methanol poisoning outbreak in Kentucky. A
clinical epidemiologic study In Archives of Environmental Health, 17(1):119-29. (1968).
[4] Paasma R., Hovda K.E., Tikkerberi A., Jacobsen D., Methanol mass poisoning
in Estonia: outbreak in 154 patients. Clin Toxicol (Phila).45(2):152-7 (2007).
[5] Balaguer J., Intoxicación por Metanol. Toxicología Clínica. Bataller Editorial,
Published in Spain p. 60-64 (2004).
[6] Colombian National Health Institute, Methanol monitoring and poisoning control
(2010). (Original language: Protocolo de vigilancia y control de intoxicaciones por
metanol, Instituto nacional de salud de Colombia (2010)).
[7] BrentJ. Fomepizole for Ethylene Glycol and Methanol Poisoning. The new england
journal of medicine 360; 21 may 21, 2009
See more of this Session: Control In Medicine and Biology
See more of this Group/Topical: Computing and Systems Technology Division
(767a) Morphological and Surface
Dependant Nano-Particle Behavior
for Medical Imaging: Evaluation of
Magnetic Nanoparticles for MRI and
Gold Nanoparticles for CT-SCAN

 Year: 2012
 Group: Nanoscale Science and Engineering Forum
 Session:
Nanotechnology for In Vivo and In Vitro Imaging
 Time:
Authors:
Reyes, J. R., Universidad de los Andes
Serrano, L. C., Universidad de los Andes
Hernandez, L. M., Universidad de los Andes
The field of medical imaging has recently enhanced its medical potential applications based
on nanotechnology; this is due in part to an extensive interest from different disciplines.
Several researchers are currently focused on the development of molecular imagenology
using nanoparticles as contrast media. These techniques have been outlined as the “non-
invasive, quantitative and replicable imaging of targeted tissues or biological processes in
vivo and ex-vivo”. The future applications of molecular imaging are expected to include:
more accurate prognoses, personalized medicine, the ability to follow the effectiveness of
treatments, and the early detection of many diseases such as cancer [1].
Current development on gold nanostructures is related to plasmon phenomena
(e.g. Photothermal ablation) and its appeal arises from their potential use as both
therapeutic agents and contrast media for several medical imaging techniques (e.g.
photoacustic imaging and CT-SCAN). On the other hand, magnetic nanoparticles have
been widely studied for magnetic resonance imaging MRI due to the alterations they induce
in the spin–spin relaxation time when introduced into biological tissues.
The aim of this study is twofold. First, to provide experimental evidence as to how the
shape of gold nanoparticles can affect the X-rays attenuation behavior. Here, this is
achieved using gold nanoshells, nanorods, hollow gold nanoparticles and colloidal gold on
controlled experiments from which the evolution of X-rays attenuation is determined.
Second, to evaluate the effects of different surface modifications on superparamagnetic iron
oxide nanoparticles (SPION´s) with the purpose of gathering information on how these
modifications can alter the spin-spin relaxation time for MRI applications. The
nanoparticles studied in this case include: magnetite capped with polyvinylpyrrolidone
(PVP), magnetite capped with citrate, magnetite capped with an amino-silane, and core-
shell magnetite-SiO2. The introduction of different chemical groups on the surface of
magnetite is expected to modify the translational diffusion of water molecules to the
magnetic core, therefore affecting the spin-spin relaxation time.
Nanoparticles are characterized by Transmission Electron Microscopy (TEM), Scanning

Electron Microscopy (SEM), Dynamic Light Scattering, Zeta potential, Fourier transform
infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and UV-VIS-NIR spectroscopy.
Their properties such as X-ray attenuation and spin-spin relaxation time are measured in
commercial medical devices for CT-SCAN tomography and MRI.
Keywords: Shape effects, surface effects, nanoparticle based biomedical imagenology,

magnetic nanoparticles, gold nanoparticles, SPION´S.
References:
[1] Thorek D. J., Chen A. K., Czupryna J., Tsourkas A.; Superparamagnetic Iron Oxide
Nanoparticle Probes for Molecular Imaging, Annals of Biomedical Engineering 34 1
,p. 23-38 (2006).
See more of this Session: Nanotechnology for In Vivo and In Vitro Imaging
See more of this Group/Topical: Nanoscale Science and Engineering Forum
(393k) Synthesis of Highly
Concentrated Gold Nanoparticles: A
Perspective for Industrial Batch
Production Processes Based On
Green Chemistry

 Year: 2012
 Session:
Session: Nanoscale Science and Engineering
 Time:
Tuesday, October 30, 2012 - 6:00pm-8:00pm
Authors:
Controlled synthesis of nanostructured materials with functional properties has been the
center of attention during the last decade because of their unique size and shape-dependent
properties (e.g. optical, electrical, magnetic, chemical, etc.). Therefore, several applications
on diverse disciplines such as medical diagnosis based on imaging and cancer treatment
research have recently been directed towards the use of nanomedicine.
Results have been successful in spite the fact that industrial scalability of these processes
nowadays is still in development, for instance the colloidal gold production with high
reproducibility and remarkable control of shape and size at concentrations in the range of
parts per million has been established; nonetheless, reproducible and facile synthesis
processes in order to obtain more concentrated colloidal dispersions of gold are yet to be
developed.
The present study evaluates three different techniques for production of highly concentrate
colloidal gold nanoparticles (hundreds of parts per million). The first synthesis is a green
chemistry approach based on the use of essential oils extracted from plants such
as pelargonium graveolens geranium and explores their use as both capping and
reductive agents. The second synthesis uses sodium citrate as the reductive agent,
polyvinylpyrrolidone (PVP) is used as capping agent, in addition, the process is carried out
at room temperature (293.15 K) and the effect of ultrasonic dispersion is also studied.
These two syntheses are contrasted with the well-known Turkevich method. It´s known that
this last traditional synthetic pathway may generate colloidal instabilities when certain
concentrations are required (> 40 ppm) due mainly to the electrical properties of the solid-
liquid interface and its relation with the zeta potential. Finally, for these three procedures
hydrogen potential (pH) and Temperature were followed rigorously in order to develop
kinetic models. Stability was monitored over time using UV-VIS-NIR spectroscopy.
The nanoparticles obtained from these techniques have been proven to be stable over
relatively long periods of time (months), making these products very useful for biomedical
applications and low cost of the reductive and capping agents used.
Nanoparticles were characterized by Transmission Electron Microscopy (TEM), Scanning

Electron Microscopy (SEM), Dynamic Light Scattering, Zeta potential, Fourier transform
infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and UV-VIS-NIR spectroscopy.
Biocompatibility of nanoparticles and their capping agents was tested in-vitro using an
MTT protocol in Chinese hamster ovarian cells (CHO), (i.e. by a cytotoxicity standard
assay).
(596am) Physiologically Based
Pharmacokinetic and
Pharmacodynamic Approach to
Nanoparticle Biodistribution and
Tumor Uptake: A Comparison
Between Gold Nanoparticles and
Spions Nanoparticles

 Year: 2012
 Group: Food, Pharmaceutical & Bioengineering Division
 Session:
Session: Engineering Fundamentals In Life Science
 Time:
Wednesday, October 31, 2012 - 6:00pm-8:00pm
Authors:
Nanomedicine and nanostructured materials research have been in exponential growth

during the last decade, several efforts have been focused on the nanoparticle design.
Nevertheless, the knowledge about nanoparticles absorption, distribution, metabolism and
excretion (ADME) has been growing more slowly, in fact these ADME parameters are
required in order to obtain the pharmacokinetic and pharmacodynamic of these nano-sized
materials.
Physiologically based pharmacokinetic models (PBPK) are mathematical strategies
developed in order to analyze ADME properties for pharmaceutical compounds, treating
the organs such as compartmental structures with several interconnections modeled by
differential equations. Therefore, the appeal of PBPKs arises from accurate prediction
models for concentration profiles by varying: doses, exposure duration and routes of
administration. In addition these models have been useful for interspecies scalability.
The aim of the current study is to provide in silico physiological based pharmacokinetic
models for the dynamic biodistribution of gold nanoparticles and superparamagnetic iron
oxide nanoparticles (SPION´s) both coated with different surface modifiers typical for each
kind of particles (dextran, polyetileneglicol, and citrate), analyzing surface charge and size
effects into the model. To date, there are few studies on how the surface charges of
nanocarriers impact their biodistribution, pharmacokinetics and tumor uptake.
The proposed models are focused on dynamic cancer treatment strategies, a comparison
between gold nanoparticles and SPION´s is developed, and issues such as liver or
lymphatic system bioaccumulation and metabolism are specially treated, using
experimental data for the most relevant organs (compartments). In addition, exploratory
models for tumor evolution are included into the model to provide a PBPK model for
chemotherapy agents and tumor-targeted substances coupled to gold nanoparticles.
The PBPK models includes separation between venous and arterial blood pools, and the
main organs for nanoparticles biodistribution have been modeled in detail (i.e. Liver, lungs,
kidneys, spleen, hearth, brain), Additionally, the parameters for this model have been
adjusted and validated using different kinds of preclinical data in mice.
See more of this Session: Session: Engineering Fundamentals In Life Science

See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division - See
also TI: Comprehensive Quality by Design in Pharmaceutical Development and
Manufacture
(596aq) Physiologically Based
Three-Compartment Model for
Anomalous Diffusion of Gold
Nanoparticles with a Pulsed Inlet

 Year: 2012
 Group: Food, Pharmaceutical & Bioengineering Division
 Session:
Session: Engineering Fundamentals In Life Science
 Time:
Wednesday, October 31, 2012 - 6:00pm-8:00pm
Authors:
The enhanced permeability and retention (EPR) effect has been explored as an appealing
route for nanoparticle transport into tummoral tissues, due to the high rate of angiogenesis
in cancerous lymphomas among other neoplasms. The EPR effect allows nanoparticles to
penetrate a high number of blood vessels in comparison to non-cancerous tissues.
Nevertheless, classical models for diffusive and convective transport of nanoparticles
through biological tissues do not achieve the level of detail needed, because there are
several restrictions related to the behavior of the mean square displacement (MSD) in such
complex media (i.e. anomalous diffusion). In addition, today little is known on how
nanoparticles properties affect individual transportation kinetic parameters [1].
This study provides a model and its numerical solution for nanoparticle diffusion in an
emulated biological blood vessel with some simplifications, modeled by a three-
compartment model, where the nanoparticles mass transport within the tissue is assumed to
take place by an anomalous diffusion process, the first compartment is a reservoir with
high concentration of gold nanoparticles and with a pulsed inlet, the second compartment is
an emulated biological tissue that allows only a one-dimensional diffusion initially with a
null concentration of nanoparticles, through this compartment nanoparticles reach a well
mixed liquid cavity with no particle at the onset of the experiment.
The effect of the pulsed behavior in the first compartment is an interesting factor that is
studied in this model due to the fact that it is not straightforward to develop a prediction of
how a pulsed inlet condition could influence the behavior of the particles MSD within the
tissue. Fractional calculus strategies arise as the natural model for these kinds of transport
phenomena because the time fractional diffusion equation (TFDE) allows the inclusion of
memory effects into the model.
The experimental set-up consists of permeation experiments conducted in a Franz diffusion

cell with rigorous controlled conditions, the nanoparticles are used without any marker
substance in order to provide an accurate prediction of the chemical interaction between the
particles and the emulated biological tissue (two types of gold nanoparticle with surface
functionalization are used: a biocompatible polymer modified surface and citrate modified
surface), the concentration inside the third compartment is measured via HR-UV-VIS
spectroscopy. Finally, a finite differences type method is used for obtaining theoretically
predicted concentrations in the third compartment, this concentrations are contrasted with
the experimental data available through a lest squares optimization which allows the
evaluation of the transport parameters related to the anomalous MSD behavior (fractional
derivative order, relaxation time and diffusivity).
Key Words: anomalous diffusion, nanoparticle diffusion, transport phenomena, gold

nanoparticles.
References:
1. Mingguang Li, Zoi Panagi, Konstantinos Avgoustakis andJoshua Reineke.
Physiologically based pharmacokinetic modeling of PLGA nanoparticles with varied
mPEG content. International Journal of Nanomedicine 2012:7 1345–1356
See more of this Session: Session: Engineering Fundamentals In Life Science
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division - See
also TI: Comprehensive Quality by Design in Pharmaceutical Development and
Manufacture
Topics:
(385g) Synthesis and
Characterization of Core-Shell
Fluorescent Nanoparticles with
Magnetic and Plasmon Properties,
for Bio-Distribution Studies and
Application In Photo-Ablation
Cancer Therapy
 Year: 2011
 Session:
Magnetic Nanoparticles In Biotechnology and Medicine
 Time:
Authors:
The development of bimetallic/bifunctional nanoparticles that exhibit a core-shell

architecture has been the focus of attention in recent years due to their unique properties in
“anti-cancer therapy’’ and other potentially attractive applications. This study presents
results on the synthesis and potential application in photo-ablation cancer therapy of a set of
core-shell bifunctional gold-coated Silica spheres with both magnetic and plasmonic
properties. Naked magnetite-maghemite Fe3O4-Fe2O3 nanoparticles are covered with a thin
shell of silica using the well-known method developed by Stöber with some modifications.
The silica-modified (magnetite-maghemite) nanoparticles are then functionalized with
Silane (APES) monolayers which are subsequently covered with a thin solid shell using Au
nanoparticles as seeds to template the growth of the shell. Also SiO2@Au, and Fe3O4-
Fe2O3@Au have been synthesized with the purpose of studying their plasmon properties; in
the SiO2@Au particles the silica core has been prepared by a novel procedure using amino-
acids as catalysts. The particles are characterized by X-ray diffraction(XRD), Dynamic
Light Scattering (DLS), Zeta potential, Scanning Electron microscopy (SEM),
Transmission electron microscopy (TEM), Atomic force microscopy (AFM), Fourier
transform infrared spectroscopy (FT-IR), and UV-VIS-NIR spectroscopy. Preliminary
results on bio-distribution (in-vitro assays) and photo-thermal ablation activity (on a
prototypical tissue) will also be presented.
(346f) Application of Core-Shell
Fluorescent Nanoparticles with
Magnetic and Plasmon Properties In
Biotransport Studies and Photo-
Ablation Cancer Therapy
 Year: 2011
 Session:
Applications of Magnetic Nanoparticles
 Time:
Authors:
Alvarez, O., Universidad de Los Andes
Bimetallic/bifunctional nanoparticles that exhibit a core-shell with precise control of size,

morphology, surface chemistry, and assembly process of each component have been the
focus of attention in recent years. These functional nanocomposite nanospheres possess a
core of silica-protected magnetite particles and in situ growth of a thin solid shell using Au
nanoparticles as seeds to template the growth of the outer layer, such nanoparticles provide,
fluorescence, magnetic and plasmon functionalities. These unique nanostructures are a
highly efficient platform for several applications. In this work two applications of such
materials will be discussed: transport in prototypical tissues and photo-ablation cancer
therapy.
For tissue bio-distribution assays we have used fluorescent nanocomposite nanopheres with
a monolayer of rhodamine encapsulated into the core of SiO2. A diffusion cell with pig skin
as prototype tissue has been implemented to conduct the transport experiments. A model
based on the concepts of anomalous diffusion and the analytic and numerical of solution of
the time fractional diffusion equation (TFDE) has been implemented, for determining the
sub-diffusion parameters that describe the transport in such complex media.
For the purpose of improving bio-distribution of the particles, and the “anti-cancer” drugs
that they could potentially transport, we have studied the change in the diffusion,
cytotoxicity, hemolytic activity, and genotoxicity; in emulsions of type (O/W) with
dispersed Fe3O4- Fe2O3@SiO2@Au nanoparticles. Finally a preliminary study of the
phothermal ablation activity of the Fe3O4-Fe2O3@SiO2@Au nanoparticles, will also be
discussed.
Topics:
(181f) Dynamic Separation of
Magnetic Nanoparticles In a
Microfluidic System with Different
Flow Conditions

 Year: 2011
 Group: Engineering Sciences and Fundamentals
 Session:
Session: Fluid Mechanics
 Time:
Monday, October 17, 2011 - 3:15pm-5:45pm
Authors:
Cuervo, D. F., Universidad de los Andes
Osma, J. F., Universidad de los Andes
Controlled magnetic-field based separation of molecules in microfluidics systems has been

the focus of attention in recent years due to their vast applications in immunological assays,
disease diagnosis and biomedical research. Therefore, studies on the development of
numerical models and their comparison with experiments of controllable separation of
magnetic nanoparticles in microfluidics systems are of great interest and are actively been
pursued. In this work, both experimental and computational results on the separation of
magnetic particles are discussed.
The experimental part of this work involves the synthesis of nanoparticles, the fabrication
of microsystems and the analysis of the system under the influence of magnetic fields with
different flow conditions. The nanoparticles used in this study are particles of magnetite,
synthesized by the coprecipitation method. Naked Fe2O3-Fe3Onanoparticles were covered
with a thin shell of silica using the well-known method developed by Stöber in order to
study core-shell nanoparticles in the microfluidics system. The silica-modified (hematite-
magnetite) nanoparticles were then functionalized with a silane monolayer. Finally due to
the importance of gold surface in biological funtionalization, core-shell nanoparticles of
Fe3O4@SiO2@Au were used in this work. The nanoparticles samples are characterized by
X-ray diffraction (XRD), Dynamic Light Scattering (DLA), Zeta potential, Scanning
Electron microscopy (SEM), Transmission electron microscopy (TEM), and Magnetic
susceptibility.
In the computational section of this work, we have simulated the magnetic-field based
separation of the nanoparticles using a multi-physics finite-element method to predict the
motion and capture of the magnetic nanoparticles as a first approximation. We also have
simulated the system as a discrete mixture of magnetic nanoparticles in a continuos media.
The simulations have been done in two different geometries and with different flow
conditions. The position and angle of the magnet have also been examined.
The simulation and experimental results are compared in order to analyze the distribution
and the efficiency of the separation of the magnetic core-shell nanoparticles. The results
show that the models developed in this study could be useful in assisting the design of
magnetic-field based separation Microsystems with potential applications on medical
diagnosis.
Topics:
Separations
(420i) Gold Nanoparticle-Based
Biosensor for Colorimetric
Detection of Helicobacter Pylori In
Water

 Year: 2011
 Session:
 Time:
Authors:
Calle, L. M., Universidad de los Andes
Bottom–up nanotechnology approaches offer a wide array of nanoparticles (NPs) with

special interest in biosensing. Gold nanoparticles (Au-NPs) have been the focus of attention
in recent years due to their unique size and shape-dependent properties (including, optical,
electrical, magnetic, catalytic, mechanical, and chemical) which make them interesting for
biological applications. Therefore, biosensing platforms based on the application of Au-
NPs are of great interest and are actively being pursued.
In this study, a colorimetric biosensor has been developed to give a rapid response for the
presence or absence of Helicobacter pylori in water based on differences in
electrostatic properties of single- and double-stranded oligonucleotides (ssDNA and
dsDNA), no functionalization of the gold, the probe, or the target DNA is involved. The
biosensor operates in two steps; the first step involves hybridization of a DNA probe with a
complementary ssDNA sequence of the target pathogen, and second; a visual detection
from colloidal gold particle aggregation for a positive result due to surface plasmon
resonance which is greatly affected by agglomeration. DNA probe, target DNA and colloid
amounts were optimized in order to improve the colorimetric readout.
Target DNA was extracted from a Helicobacter Pylori culture and denatured to obtain
ssDNA, a lyophilized oligonucleotide complementary sequence was purchased and used as
received, salt solution of given ionic strength was used to screen the repulsive interactions
of Au-NPs and induced the aggregation. Colloidal gold was synthesized by means of citrate
reduction of HAuCl4 (Turkevich method). Gold nanoparticles were characterized by
Scanning Electron microscopy (SEM), Ultraviolet-visible spectroscopy, Fourier transform
infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Atomic force
microscopy (AFM). This simple colorimetric hybridization assay has also been integrated
into a disposable microfluidic device for simple widespread use. The results show that this
relatively simple assay is surprisingly robust in its ability to detect the presence of the target
pathogen.
(193n) Anomalous Diffusion of
Magnetic Nanoparticles On a
Microfluidic Sytem Under a Variable
Magnetic Field
 Year: 2011
 Group: Engineering Sciences and Fundamentals
 Session:
Session: Thermodynamics and Transport Properties
 Time:
Monday, October 17, 2011 - 6:00pm-8:00pm
Authors:
Microfluidic devices has been the focus of attention in different areas, such as chemistry,
biology, bioengineering and health sciences in past few years due to the numerous
advantages in sample preparation, characterization and analysis. Despite of these
characteristics, the separation and mixing of particles or fluids are quite difficult to achieve
in such devices due to the diffusive regimen. As a possible solution to this problem, some
authors have proposed magnetic-field based separation and mixing. As a result, studies on
the behavior of diffusion as a function of the applied magnetic field on this type of systems
are of great interest.
In this work, we have studied the behavior of an aqueous suspension of magnetic
nanoparticles flowing through a microfludic system in the presence of an externally
imposed magnetic field. We have measured the concentration profile across the
microsystem and adjusted the diffusion coefficient to a non-regular diffusive regime.
The experimental work has involved the synthesis of magnetic nanoparticles and the
analysis of particles transport under the influence of different magnetic fields and flow
conditions. The nanoparticles used in this study are particles of magnetite, synthesized by
the coprecipitation method and stabilized with an electrolyte to screen electrostatic
interactions. Core-shell magnetic nanoparticles are also considered. In order to verify our
model we have develop numerical simulations using COMSOL Multiphysics to corroborate
the behavior of the microfluidic system and the concentration profile.
The simulation and experimental results are compared in order to analyze the non-regular
diffusive regime. The results obtained indicate an anomalous diffusion regime in the
presence of an external magnetic field in this type of systems.
(648d) Preparation and
Characterization of Funtionalized
Hematite-Magnetite Nano-Particles
for the Anticancer Drug Delivery of
Cisplatin
 Year: 2010
 Session:
Magnetic Nanoparticles in Biotechnology and Biomedicine II
 Time:
Authors:
Controlled fabrication of nanostructured materials with functional properties has been the
focus of attention in recent years due to their unique size and shape-dependent properties
(optical, electrical, magnetic, catalytic, mechanical, chemical, etc.). Therefore, studies on
the controllable synthesis of nanomaterials are of great interest and are actively being
pursued.
In this study, we have attached the active anticancer drug cisplatin to a silica-modified
(hematite-magnetite) nanoparticle for improved drug delivery. Naked Fe2O3-Fe3O4
nanoparticles were covered with a thin shell of silica using the well-known method
developed by Stöber. The silica-modified (hematite-magnetite) nanoparticles were then
functionalized with a silane monolayer. Cisplatin was then added to the silanated surface to
yield a complex functionalized nanoparticle. The functionalized Fe2O3-Fe3O4 samples are
characterized by X-ray diffraction, Dynamic Light Scattering, Zeta potential, Scanning
Electron microscopy (SEM), Transmission electron microscopy (TEM), and Nuclear
Magnetic Resonance (NMR).
The platinum-tethered nanoparticles were also examined for in-vitro cytotoxicity (using a
hemolysis assay), drug uptake, and localization (by fluorecent tagging) in colorectal
adenocarcinoma cells. The results obtained show that nanoparticles developed in this study
could potentially be useful in the controlled delivery of cisplatin for cancer therapeutics and
may be considered a promising platform in targeted therapy of cancer.
(373c) Core-Shell Magnetic
Nanoparticles and Their Hemolytic
Activity
 Year: 2010
 Session:
 Time:
Tuesday, November 9, 2010 - 6:00pm-8:00pm
Authors:
Bimetallic nanoparticles that exhibit a core-shell architecture have been the focus of
attention in recent years due to their unique size and shape-dependent properties (optical,
electrical, magnetic, catalytic, mechanical, chemical, etc.), when compared with their
monometallic counterparts. Introducing a magnetic element (core) in nanoparticles is
additionally attractive owing to their potential applications in different fields. Therefore,
studies on the controllable synthesis of nanomaterials with a core-shell structure are of
great interest and are actively being pursued.
In this study, a two-step seeding growth process has been used to produce silica-modified
hematite-magnetite nanoparticles as platforms for targeted drug delivery. Naked Fe2O3-
Fe3O4 nanoparticles obtained by a co-precipitation process were covered with a silica shell
of variable thickness using the well-known method developed by Stöber. The silica-
modified hematite-magnetite nanoparticles were then functionalized with a silane
monolayer. The functionalized Fe2O3-Fe3O4 samples are characterized by X-ray
diffraction, Dynamic Light Scattering, Zeta potential, Scanning Electron microscopy
(SEM), Transmission electron microscopy (TEM), and Nuclear Magnetic Resonance
(NMR).
This study also uses the measure of hemolysis to evaluate the toxicity of silica-modified
hematite-magnetite nanoparticles with and without surface modification and with varied
sizes and investigates the effects on the nanoparticle-cell interaction.
Topics:
Nanotechnology
Respetado/a:
Angel Galvis C.:
Reciba un cordial saludo del Comité Técnico de este XV Congreso Colombiano de Petróleo y Gas: “Innovación y desarrollo sostenible:
El futuro del país”.
Nos complace comunicarle que su trabajo: “Functional Advanced Material Development For Continuos Oil-Water Separation”
ha sido seleccionado para presentación oral durante el Congreso. El espacio para su presentación será notificada durante el mes
de octubre.
Recuerde que debe estar una hora antes del horario establecido, de lo contrario se entenderá que no habrá presentación y
el tiempo asignado debe ser cumplido estrictamente y confirmado con anterioridad. Le agradecemos hacernos llegar antes del
13 de Noviembre de 2013, una copia de la presentación correspondiente a su trabajo, adicionando una corta reseña biográfica
del autor para su presentación, al correo electrónico: congreso@acipet.com. Usted contará con todas las herramientas audiovisuales
necesarias para el desarrollo de su presentación, incluso un salón en el que podrá hacer prueba de su presentación previamente. S
i por algún motivo, usted no puede presentar su conferencia, le pedimos el favor de notificar lo antes posible al mismo correo.
Aprovechamos la oportunidad para agradecerle su interés en ser partícipe de este importante evento.
Cordialmente, Ing. Emiliano Mejía Duque Director Comité Técnico XV Congreso Colombiano de Petróleo y Gas.
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PRESENTACIÓN ORAL XV CCPG
3
NP
Nathalie Pinzón<congreso@acipet.com>
Responder a todos|
mar 05/11/2013, 12:41 p.m.
Angel Alberto Galvis Caballero
Bandeja de entrada
CORREGIMOS EL SALÓN ES EL 4
Reciba un cordial saludo del Comité Técnico de este XV Congreso Colombiano de Petróleo y
Gas: “Innovación y desarrollo sostenible: El futuro del país”.
Nos complace comunicarle que su trabajo: “Functional advanced material development for
continuos Oil-Water Separation” ha sido seleccionado para presentación oral durante el Congreso.
El espacio para su presentación ha sido programado para el Jueves 21 de Noviembre de
2013 de 09:10am a 09:40am, en el salón 4 del segundo nivel del Pabellón 11 al 16.
Cordialmente,
Nathalie Pinzón Pérez
Comité Técnico
Asociación Colombiana de Ingenieros de Petróleos – ACIPET
Cra. 11 A # 94 A – 56 Piso 4 – Bogotá, Colombia
Tel: 6 411 944 ext. 123
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