BIOL 3132 – Overview of Topic Information (excerpt from Topic and Lab manual)

STRUCTURE OF THE TOPIC

Protein to the proteome is composed of 34 lectures which be held on Monday, at 12pm and
Tuesday and Wednesday at 9am in Room PYHS 0009. Tutorials with Drs Soole and Menz will
be held either Tuesdays in PYHS 0006 or Wednesday at 10am in PHYS 0005. Each Student will
have 4 tutes during the topic in either Tuesdays or Wednesday. This will depend on what tute
time group you have enrolled in online. Within Each tute time slot there are assigned tute groups
and you should attend with your group as outlines in tute timetable below. Tutes begin in week
2. The subject of these tutorials will be research papers chosen by the lecturers and distributed
before the tutes begin. Practicals will be held either Wednesday or Thursday 11am to 5pm in
weeks1, 3,5,7, 9 and 11. There will be two whole class post-laboratory tutorials. Practicals start
in Week 1.

LECTURE SCHEDULE

To be held in room Phys 0009.

WEEK DATE LECTURE TOPIC

1 1 Monday 26.7 Intro lecture/IM1

Protein structure X-ray crystallography
2 Tuesday 27.7 KAS 1 Methods of protein purification 1
3 Wednesday 28.7 KAS 2 Methods of protein purification 2
2 4 Monday 2.8 KAS 3 Methods of protein purification 3
5 Tuesday 3.8 KAS 4 Methods of protein purification/ western Blotting
6 Wednesday 4.8 KAS 5 Inhibition kinetics
3 7 Monday 9.8 IM2 Protein structure NMR spec
8 Tuesday 10.8 IM3 Protein structure AFM/Cryo
9 Wednesday 11.8 IM4 Interpretation Protein structure
4 10 Monday 16.8 IM 5 Yeast 2-Hybrid
11 Tuesday 17.8 IM 6 SPR calorimetry
Wednesday 18.8 IM 7 Mass spectrometry analysis of proteins
5 12 Monday 23.8 IM 8 Proteomic analysis of tissues 1
13 Tuesday 24.8 IM 9 Proteomic analysis of tissues 2
14 Wednesday 25.8 IM 10 Bioinformatic tools for protein analysis – homology
modelling
6 15 Monday 30.8 IM 11 Protein docking and in silico drug screening
16 Tuesday 31.8 CAA 1
17 Wednesday 1.9 CAA2
7 18 Monday 6.9 CAA3
19 Tuesday 7.9 CAA4
20 Wednesday 8.9 CAA5
8 21 Monday 13.9 CAA 6

22 Tuesday 14.9 CAA 7

 23 Wednesday 15.9 LS 1

MID SEMESTER BREAK

9 5.10 PUBLIC HOLIDAY

24 Tuesday 5.10 LS 2
25 Wednesday 6.10 LS 3
10 26 Monday 11.10 LS 4
27 Tuesday 12.10 KLS 1
28 Wednesday KLS 2
13.10
11 29 Monday 18.10 KLS 3
30 Tuesday 19.10 KLS 4
31 Wednesday KLS 5
20.10
12 32 Monday 25.10 KLS 6
33 Tuesday 26.10 KLS 7
34 Wednesday KLS 8
27.10

TUTORIAL SCHEDULE

All tutes held in room Phys 0006, except * Phys 0007 and ** Phys0006

WEEK DATE Tute Group

1 Tuesday 27.7 NO TUTES

Wednesday 28.7 NO TUTES
2 Tuesday 3.8 NO TUTES
Wednesday 4.8 NO TUTES
3 Tuesday 10.8 Group 1, IM Tute Paper
Wednesday 11.8 Group 5, IM tute paper
4 Tuesday 17.8 Group 2 IM Tute paper
Wednesday 18.8 Group 6 IM Tute Paper
5 Tuesday 24.8 Group 3 IM Tute Paper
Wednesday 25.8 Group 7 IM Tute Paper
6 Tuesday 31.8 Group 1 KLS Tute Paper AND Group 4, IM Paper*
Wednesday 1.9 Group 5, KLS Tute Paper AND Group 8, IM Paper **
7 Tuesday 7.9 Wed Prac Group Practical Tutorial
Wednesday 8.9 Thursday Prac Group Practical Tutorial
8 Tuesday 14.9 Group 2, KLS Tute Paper
Wednesday 15.9 Group 6 KLS Tute Paper

SEMESTER BREAK

9 Tuesday 4.10 Group 3 KLS Tute paper

Wednesday 5.10 Group 7KLS Tute Paper
10 Tuesday 12.10 Group 4 KLS Tute Paper
Wednesday Group 8 KLS Tute Paper
13.10
11 Tuesday 19.10 Wed Prac Group Practical Tutorial
 Wednesday Thursday Prac Group Practical Tutorial
20.10
12 Tuesday 26.10 Wed Prac Group Practical Tutorial
Wednesday Thursday Prac Group Practical Tutorial
27.10

OUTLINE OF LABORATORY WORK

SESSION 1 (28th, 29th July)

Protein Purification 1, Solution preparation (Labs 6 and 7)
Molecular Graphics Tutorial ( in CML Lab)
SESSION 2 (12th ,13th August)
Protein Purification 2
SESSION 3 (25th ,26th August)
Protein Purification 3
Protein Estimation Skills Test
Balance Sheet for Purification
SESSION 4 (8th, 9th Sept. )
This week there will be a post-laboratory tutorial
Gel Electrophoresis
Enzyme Kinetics
Protein Estimation skills test
SESSION 5 (6th , 7th Oct)
Western Blot 1
Enzyme Kinetics (needs to be completed this session)
SESSION 6 (21st, 22nd Oct)
Western Blot 2
Interpretation of Protein Structure (in CML)