Integration of the Michaelis-Menten Equation

𝑑[𝑆] 𝑘2 [𝐸]0 [𝑆]

𝑟=− =
𝑑𝑡 𝐾𝑀 + [𝑆]
𝐾𝑀 + [𝑆]
𝑑[𝑆] = −𝑘2 [𝐸]0 𝑑𝑡
[𝑆]
[𝑆]1 𝑡
𝐾𝑀 + [𝑆]
∫ 𝑑[𝑆] = − ∫ 𝑘2 [𝐸]0 𝑑𝑡
[𝑆]0 [𝑆] 0

[𝑆]1 [𝑆]1
𝐾𝑀
∫ 𝑑[𝑆] + ∫ 𝑑[𝑆] = −𝑘2 [𝐸]0 𝑡
[𝑆]0 [𝑆] [𝑆]0

[𝑆]1
𝐾𝑚 ln + ([𝑆]1 − [𝑆]0 ) = −𝑘2 [𝐸]0 𝑡
[𝑆]0
[𝑆]1 − [𝑆]0 𝑘2 [𝐸]0 𝑡
𝐾𝑚 + =−
[𝑆]1 [𝑆]
ln ln 1
[𝑆]0 [𝑆]0
[𝑆]0 − [𝑆]1 𝑘2 [𝐸]0 𝑡
∴ = 𝐾𝑚 +
[𝑆] [𝑆]
ln 1 ln 1
[𝑆]0 [𝑆]0

Eadie-Hofstee Manipulation
𝑉𝑚𝑎𝑥 [𝑆]
𝑟=
𝐾𝑀 + [𝑆]
1 𝐾𝑀 + [𝑆]
=
𝑟 𝑉𝑚𝑎𝑥 [𝑆]
𝑉𝑚𝑎𝑥 𝐾𝑀 + [𝑆]
= 𝑉𝑚𝑎𝑥 ( )
𝑟 𝑉𝑚𝑎𝑥 [𝑆]
𝐾𝑀 + [𝑆]
𝑉𝑚𝑎𝑥 = 𝑟( )
[𝑆]
𝑟𝐾𝑀 𝑟[𝑆]
𝑉𝑚𝑎𝑥 = +
[𝑆] [𝑆]
𝑟𝐾𝑀
𝑉𝑚𝑎𝑥 = +𝑟
[𝑆]
𝑟
∴ 𝑟 = −𝐾𝑀 + 𝑉𝑚𝑎𝑥
[𝑆]
Hanes – Woolf Manipulation
 𝑉𝑚𝑎𝑥 [𝑆]
𝑟=
𝐾𝑀 + [𝑆]
1 𝐾𝑀 + [𝑆]
=
𝑟 𝑉𝑚𝑎𝑥 [𝑆]
1 𝐾𝑀 1 [𝑆]
= +
𝑟 𝑉𝑚𝑎𝑥 [𝑆] 𝑉𝑚𝑎𝑥 [𝑆]
[𝑆] [𝑆]𝐾𝑀 1 [𝑆][𝑆]
= +
𝑟 𝑉𝑚𝑎𝑥 [𝑆] 𝑉𝑚𝑎𝑥 [𝑆]
[𝑆] 1 𝐾𝑀
∴ = [𝑆] +
𝑟 𝑉𝑚𝑎𝑥 𝑉𝑚𝑎𝑥