Urey ost Toms e ‘The equation holds true for freezing temperature, The solute potential ofthe extracted sap «at room temperature can then be computed: Equation (1-7): y= 1.22Ty¢ Where: cis the correction factor, (Rm temp in K/273K) B.1 Sap extraction 1. Peel 2-4 potatoes, chop and place in a blender. Puree the tissue, 2, Filter blended potato with cheesecloth to remove cell wall and debris, Store in covered beaker, 8.2 Freezing point detormination 3. Immerse Heidenhain thermometer in crushed ice ~ salt bath. Record reading, 4 Pour 60m sap in 260m! Erlenmeyer ‘ask with magnetic stiring bar and insert thermometer, Surround flask with ice-salt bath (see Figure 11). Str vigorously. ‘5. When temperature reads about 1°C, read temperature every 10 sec. NOTE: There should be a continuous drop in temperature, followed by a short plateau which corresponds to the freezing point, i folowed by an increase 66. Record data on Table 1-2 and construct temperature vs. time graph, 7. Determine the true freezing point, T, Equation (1-8): T, = T,'~ 0.0125, where: Tj'= apparent freezing point 4, = degrees of supercoaling: (in sign 0.0125 = amount of water (1/80) solidtying per degree of supercooting 8, Make another correction for the zero point ofthe thermometer. 8, Determine the solute potential using Equation (1-5) at 0°C, 10, Caleulate the pressure potential (¥) of the cells of potate.Do the same for all plant samples.