Name: Eric Cristian C.

Juniosa

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1. Which of the following is true of osmosis?

You correctly answered: c. It is a type of diffusion.

2. Which of the following occurs when a hypertonic solution is added to cells?

You correctly answered: d. The cells shrink.

3. The variable that affects osmotic pressure is

You correctly answered: a. the concentration of nondiffusing solutes.

4. The net movement of water would be into the cell in a

You correctly answered: b. hypotonic solution.

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Experiment Results
Predict Question:
Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will have?
Your answer : c. increased diffusion rate

Predict Question 2: What do you think will be the pressure result of the current experimental conditions?
Your answer : c. pressure above the left beaker

Stop & Think Questions:

Why do you think there is no pressure change?
You correctly answered: b. Sodium is able to diffuse through the pores.

Why do you think there was no pressure change?

You correctly answered: c. There is no net movement of water because the solute concentrations are the same.

Experiment Data:

Run Number Solute MWCO Start Conc. L Pres. L Start Conc. R Pres. R Rate
1 Na+ Cl- 20 5.00 170 0.00 0 0.0000
1 Albumin 20 0.00 170 0.00 0 0.0000
1 Glucose 20 0.00 170 0.00 0 0.0000
2 Na+ Cl- 20 10.00 340 0.00 0 0.0000
2 Albumin 20 0.00 340 0.00 0 0.0000
2 Glucose 20 0.00 340 0.00 0 0.0000
3 Na+ Cl- 50 10.00 0 0.00 0 0.0167
3 Albumin 50 0.00 0 0.00 0 0.0000
3 Glucose 50 0.00 0 0.00 0 0.0000
4 Na+ Cl- 100 0.00 136 0.00 0 0.0000
4 Albumin 100 0.00 136 0.00 0 0.0000
4 Glucose 100 8.00 136 0.00 0 0.0000
5 Na+ Cl- 100 0.00 136 0.00 0 0.0000
5 Albumin 100 0.00 136 0.00 0 0.0000
5 Glucose 100 8.00 136 0.00 0 0.0000
6 Na+ Cl- 200 0.00 0 0.00 0 0.0000
6 Albumin 200 0.00 0 0.00 0 0.0000
6 Glucose 200 8.00 0 0.00 0 0.0036
7 Na+ Cl- 200 0.00 153 0.00 0 0.0000
7 Albumin 200 9.00 153 0.00 0 0.0000
7 Glucose 200 0.00 153 10.00 0 0.0044

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Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic pressure will
You correctly answered: c. double.

2. Which membrane did not allow Na+ Cl- to pass through?

You correctly answered: a. 20 MWCO

3. When a solute is able to diffuse through a membrane

You correctly answered: b. equilibrium is reached.

4. Water diffuses
You correctly answered: c. toward solutes.

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Review Sheet Results
1. Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and why it has this effect. How well
did the results compare with your prediction?
Your answer:
Osmotuc pressure is the pressure needed to go against the diffusion of water (via osmosis) into a region of higher solute
concentration. A higher solute concentration draws water more strongly so the osmotic pressure needed to go against the
diffusion of water because solutes draw water toward them.

2. Describe one way in which osmosis is similar to simple diffusion and one way in which it is different.
Your answer:
An example of simple diffusion is osmosis in such a way that both processes are passive. On the other hand, it is different
from simple diffusion in such a way that in osmosis, a selectivley permeable membrane is involved and the main concern is
the movement of the water. In general, simple diffusion does not involve necessarily a membrane. it usually considered in
terms of solutes rather than water.

3. Solutes are sometimes measured in milliosmoles. Explain the statement, “Water chases milliosmoles.”
Your answer:
Water moves towards a region of higher concentration where there is an increase in the amount of milliosmoles.

4. The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO
membrane in place. Explain the results. How well did the results compare with your prediction?
Your answer:
Following the concentration gradient, the results were half the glucose diffused from the right beaker to the left beaker for
the latter had a higher concentration of solutes. Albumin did not move because it was too large to get through the pores of
the filter.

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