Forensic Science International 139 (2004) 89–92

Announcement of population data

Genetic data on 19 STR loci in south-east Poland
Piotr Kozioła,*, Marzanna Ciesielkaa, Roman Ma˛droa,
Andrzej Krajkab
a
Department of Forensic Medicine, University School of Medicine in Lublin, ul. Jaczewskiego 8, 20-090 Lublin, Poland
b
Department of Mathematics, University M. Curie-Skłodowska in Lublin, pl. M. Curie-Skłodowskiej 2, 20-080 Lublin, Poland
Received 31 July 2003; received in revised form 1 October 2003; accepted 3 October 2003

Abstract

Allele frequencies for the 19 STRs loci, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11,
CSF1PO, F13A01, F13B, FESFPS, FGA, LPL, Penta D, Penta E, TH01, TPOX and VWA were obtained from a sample of 203–
1188 unrelated individuals living in the area of south-east Poland.
# 2003 Elsevier Ireland Ltd. All rights reserved.

Keywords: STRs; Population database; Polish population

Population: Unrelated individuals living in the region of
south-east Poland who requested for paternity testing.
Extraction: Genomic DNA was isolation from blood sam-
ples by the Blood DNA Prep Plus kit (A&A Biotechnology).
PCR: One to five nanograms target DNA following to
manufacturer’s instructions (GenePrint1 Fluorescent STR
Systems, Promega).
Typing: Hitachi FMBIO II fluorescent scanner, ILS 600
as internal standard and allelic ladder (Promega).
Results: See Table 1
Quality control: Proficiency testing of the Paternity
Testing Workshop—ISFG.
Analysis of data: Hardy–Weinberg equilibrium,
observed and expected heterozygosity were calculated
within TFPGA of Miller [1], polymorphism information
content (PIC) [2], power of discrimination (PD) [3], power
of exclusion (PE) [4], minimum allele frequencies [5].
Other remarks: The alleles frequency comparisons
between our region and other regions of Poland: 19 mar-
kers—South [6–8]; 17 markers—North [9,10], North East
[11,12], Central Pomarania Kujawy [13,14]; 8 markers—
Central Warsaw [15,16], were performed by test of homo-
geneity (RxC contingency table test with 2000 shuffling
experiments—data not shown). Comparison of data shown
regional differences (P < 0:05) at the FGA locus (between
our data and North, North East), at the D21S11 locus
(between our data and North East) and at the CSF1PO locus
(between our data and Central Pomarania Kujawy, North
East). In other comparisons no differences found. This paper
follows the guidelines for publication of population data
requested by the journal [17].
Access to the data: Available upon request: pkoziol
@asklepios.am.lublin.pl.

*
Corresponding author. Tel.: þ48-81-7425794;
fax: þ48-81-7476427.
E-mail address: pkoziol@asklepios.am.lublin.pl (P. Kozioł).

0379-0738/$ – see front matter # 2003 Elsevier Ireland Ltd. All rights reserved.
doi:10.1016/j.forsciint.2003.10.001
90 P. Kozioł et al. / Forensic Science International 139 (2004) 89–92

Table 1
Allele frequencies for 19 STR systems in the population of south-east Poland

Allele D3S1358 D5S818 D7S820 D8S1179 D13S317 D16S539 D18S51 D21S11 CSF1PO
(N ¼ 714) (N ¼ 515) (N ¼ 517) (N ¼ 714) (N ¼ 517) (N ¼ 517) (N ¼ 714) (N ¼ 714) (N ¼ 496)

3.2 – – – – – – – – –
4 – – – – – – – – –
5 – – – – – – – – –
6 – – – – 0.0010 – – – –
7 – 0.0019 0.0097 – – – – – –
8 – 0.0010 0.1625 0.0091 0.1112 0.0068 – – 0.0020
9 – 0.0544 0.1518 0.0147 0.0832 0.0880 – – 0.0615
9.3 – – – – – – – – –
10 – 0.0854 0.2979 0.0644 0.0493 0.0571 0.0070 – 0.2822
11 0.0014 0.3505 0.1992 0.0735 0.3811 0.2921 0.0189 – 0.2792
12 – 0.3447 0.1557 0.1723 0.2398 0.3211 0.0945 – 0.2944
13 0.0028 0.1485 0.0193 0.3116 0.0861 0.1905 0.1037 – 0.0655
14 0.1478 0.0126 0.0039 0.2332 0.0435 0.0425 0.1695 – 0.0071
15 0.2255 – – 0.1016 0.0048 0.0019 0.1555 – 0.0081
16 0.2430 0.0010 – 0.0182 – – 0.1639 – –
17 0.2220 – – 0.0007 – – 0.1134 – –
18 0.1435 – – 0.0007 – – 0.0805 – –
19 0.0119 – – – – – 0.0392 – –
20 0.0021 – – – – – 0.0336 – –
20.2 – – – – – – – – –
21 – – – – – – 0.0126 – –
21.2 – – – – – – – – –
22 – – – – – – 0.0070 – –
22.2 – – – – – – – – –
23 – – – – – – 0.0007 – –
23.2 – – – – – – – – –
24 – – – – – – – – –
24.2 – – – – – – – – –
25 – – – – – – – – –
25.2 – – – – – – – – –
26 – – – – – – – 0.0021 –
27 – – – – – – – 0.0315 –
28 – – – – – – – 0.1891 –
28.2 – – – – – – – 0.0007 –
29 – – – – – – – 0.1926 –
29.2 – – – – – – – 0.0028 –
30 – – – – – – – 0.2458 –
30.2 – – – – – – – 0.0469 –
31 – – – – – – – 0.0518 –
31.2 – – – – – – – 0.0855 –
32 – – – – – – – 0.0147 –
32.2 – – – – – – – 0.0882 –
33 – – – – – – – 0.0021 –
33.2 – – – – – – – 0.0420 –
34.2 – – – – – – – 0.0042 –
Min. freq. 0.0037 0.0049 0.0051 0.0038 0.0050 0.0051 0.0040 0.0039 0.0053
Hobs 0.786 0.709 0.768 0.814 0.754 0.762 0.878 0.840 0.756
Hexp 0.799 0.726 0.798 0.799 0.767 0.763 0.878 0.844 0.748
Pw2 0.93 0.99 0.33 0.30 0.41 0.92 0.71 0.99 0.66
Pexact 0.64 0.99 0.18 0.37 0.46 0.85 0.46 0.84 0.29
PIC 0.767 0.680 0.768 0.771 0.736 0.725 0.865 0.826 0.704
PD 0.929 0.884 0.929 0.929 0.912 0.905 0.971 0.958 0.886
PE 0.596 0.491 0.600 0.611 0.568 0.546 0.753 0.692 0.514
 P. Kozioł et al. / Forensic Science International 139 (2004) 89–92 91

Table 1 (Continued )

F13A01 F13B FESFPS FGA LPL Penta D Penta E TH01 TPOX VWA
(N ¼ 308) (N ¼ 251) (N ¼ 308) (N ¼ 714) (N ¼ 351) (N ¼ 203) (N ¼ 712) (N ¼ 1132) (N ¼ 1188) (N ¼ 1078)

3.2 0.0747 – – – – – – – – –
4 0.0390 – – – – – – – – –
5 0.1786 – – – – – 0.0709 0.0013 – –
6 0.3117 0.0916 – – – – – 0.2253 0.0008 –
7 0.3442 0.0159 0.0016 – 0.0014 0.0025 0.1376 0.1387 – –
8 0.0032 0.2092 0.0292 – 0.0028 0.0049 0.0169 0.1016 0.5783 –
9 – 0.2450 0.0032 – 0.0470 0.2414 0.0119 0.2107 0.0825 –
9.3 – – – – – – – 0.3127 – –
10 – 0.4343 0.2565 – 0.4430 0.1158 0.1166 0.0088 0.0653 –
11 0.0016 0.0040 0.4806 – 0.2308 0.1355 0.1096 0.0009 0.2445 0.0014
12 – – 0.1916 – 0.2522 0.2118 0.1355 – 0.0286 –
13 0.0049 – 0.0373 – 0.0214 0.1946 0.1173 – – 0.0051
14 0.0097 – – – 0.0014 0.0566 0.0667 – – 0.1039
15 0.0162 – – – – 0.0246 0.0562 – – 0.1109
16 0.0162 – – 0.0007 – 0.0123 0.0506 – – 0.1878
17 – – – 0.0007 – – 0.0400 – – 0.2551
18 – – – 0.0063 – – 0.0351 – – 0.2324
19 – – – 0.0833 – – 0.0183 – – 0.0918
20 – – – 0.1520 – – 0.0126 – – 0.0116
20.2 – – – 0.0007 – – – – – –
21 – – – 0.1737 – – 0.0028 – – –
21.2 – – – 0.0014 – – – – – –
22 – – – 0.2346 – – – – – –
22.2 – – – 0.0112 – – – – – –
23 – – – 0.1127 – – 0.0014 – – –
23.2 – – – 0.0126 – – – – – –
24 – – – 0.1191 – – – – – –
24.2 – – – 0.0028 – – – – – –
25 – – – 0.0672 – – – – – –
25.2 – – – 0.0007 – – – – – –
26 – – – 0.0147 – – – – – –
27 – – – 0.0056 – – – – – –
28 – – – – – – – – – –
28.2 – – – – – – – – – –
29 – – – – – – – – – –
29.2 – – – – – – – – – –
30 – – – – – – – – – –
30.2 – – – – – – – – – –
31 – – – – – – – – – –
31.2 – – – – – – – – – –
32 – – – – – – – – – –
32.2 – – – – – – – – – –
33 – – – – – – – – – –
33.2 – – – – – – – – – –
34.2 – – – – – – – – – –
Min. freq. 0.0083 0.0099 0.0079 0.0039 0.0072 0.0133 0.0041 0.0023 0.0020 0.0025
Hobs 0.734 0.693 0.646 0.840 0.715 0.817 0.900 0.762 0.583 0.822
Hexp 0.746 0.700 0.665 0.853 0.685 0.825 0.905 0.778 0.594 0.814
Pw2 0.99 0.82 0.14 0.79 0.85 0.83 0.43 0.66 0.49 0.68
Pexact 0.90 0.70 0.10 0.71 0.75 0.63 0.64 0.66 0.40 0.43
PIC 0.704 0.649 0.611 0.836 0.629 0.799 0.896 0.742 0.544 0.788
PD 0.892 0.860 0.831 0.961 0.839 0.943 0.982 0.917 0.783 0.938
PE 0.521 0.451 0.411 0.706 0.428 0.648 0.806 0.565 0.356 0.631
Hobs (observed heterozygosity), Hexp (expected heterozygosity), Pw2 (Hardy–Weinberg equilibrium, probability of chi-square test), Pexact
(Hardy–Weinberg equilibrium, probability of exact test based on 10,000 total permutations), PIC (polymorphism information content), PD
(power of discrimination), PE (power of exclusion), N (number of samples), min. freq. (minimum frequency).
92 P. Kozioł et al. / Forensic Science International 139 (2004) 89–92
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