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2
ATOMIC ABSORPTION SPECTROMETRY: INSTRUMENTATION,
ABSORPTION/ EMISSION TECHNIQUES
I. Introduction
Measurements based on light and other forms of electromagnetic radiation are widely
used in analytical chemistry. The science that deals with interactions of radiation and matter is
known as spectroscopy. Spectroscopy has played a major role in the development of the modern
atomic theory as well as has provided the tools for molecular structure elucidation and both
qualitative and quantitative determination of both inorganic and organic compounds (Harvey,
2000).
In FAAS, atomic absorption occurs when a ground state atom absorbs energy in the form
of light of a specific wavelength and is elevated to an excited state. In GFAAAS, the sample is
introduced into a graphite tube, which is then heated in a programmed series of steps to remove
the solvent and major matrix components and to atomize the remaining sample. On the other
hand, ICP-OES is the measurement of the light emitted by the elements in a sample introduced
into an OCP source. The measured emission intensities are then compared to the intensities of
standards of known concentration to obtain the elemental composition in the unknown sample.
Atomization is the first step in all atomic spectroscopic procedures since spectroscopic
determination of atomic species can only be performed on a gaseous medium in which the
individual atoms or elementary ions are well separated from one another. Atomization is the
process in which a sample is volatilized and decomposed in such a way to produce gas-phase
atoms and ions. Absorption of light in this analytical technique is accomplished for the "free
atoms". Atomization process, moreover, is consist of five successive stages namely mixing,
evaporation, fusion, vaporization and atomization.
There are two ways of introducing a standard in analytical process. In external standard,
reference standards of known concentration are produced together with a reference blank of zero
concentration. On the other hand, internal standard involves the addition of a known amounts of
the standard to the unknown sample wherein the change in response of the unknown sample is
recorded (Harvey, 2000). Furthermore, possible interferences could occur in analyzing sample
via AAS. These include spectral, chemical, and ionization interferences.
This study aims to (1) study the different components of an AAS System: absorption vs
emission modes, (2) determine the effects of releasing agents and/or ionization suppressants,
and (3) familiarize the standard addition techniques for trace metal analysis.
The components of an atomic absorption spectrometer are the light source (A), which
emits the characteristic narrow-line spectrum of the element of interest; an absorption cell or
atom reservoir in which the atoms of the sample to be analyzed are formed by thermal molecular
dissociation, most commonly by a flame (B); a monochromator (C) for the spectral dispersion of
the light into its component wavelengths with an exit slit of variable width to permit selection and
isolation of the analytical wavelength; a photomultiplier detector (D) whose function is to convert
photons of light into an electrical signal which may be amplified (E) and eventually displayed to
the operator on the instruments readout (F) (Cantle, 1982).
The first component of the atomic absorption spectrometer is the atomizer. The atomizer
breaks sample into atoms to observe atomic spectra. The most commonly used atomizer is the
chemical flame, based upon the combination of a fuel gas (e.g., acetylene) with an oxidant (e.g.,
air or nitrous oxide). The sample solution is introduced into the flame using a nebulizer in which
the passage of the oxidant creates a partial vacuum by the venture effect and thus the sample
solution is drawn up through a capillary. Thus, an aerosol is produced having a wide variety of
droplet sizes. The parts of an atomizer are shown in Figure 2.7.
Figure 2.7. Cross-section of premix chamber of a flame atomizer.
(Image obtained from Cantle, J.E. 1982. Atomic Absorption Spectrometry.
New York: Elsevier Science Publishing Company Inc., p. 17.)
There are several types of nebulizers and atomizers. Nebulizers may be concentric-tube
pneumatic effect nebulizers, cross-flow nebulizer, fritted disk nebulizer and Babington nebulizer.
Moreover, the following are the types of atomizers for atomic spectroscopy and their
corresponding typical atomization temperature.
There are several factors are to be considered in choosing and optimizing flame atomizers
such as the proper alignment of the flame in the light path and the type of flame used. The ideal
flame for atomic absorption should generate the correct amount of thermal energy to dissociate
the atoms from their chemical bonds. The most commonly used flames are air-acetylene and
nitrous oxide-acetylene, which produce flame temperatures of about 2300°C and 2950°C,
respectively. These types of flames are used in the analysis of 30 elements for the air acetylene
and 66 elements for nitrous oxide-acetylene. In addition, the fuel and oxidant mixture must be
controlled to provide the proper flame conditions for the element being analyzed. This is
accomplished by a gas control system providing the precise and safe regulation which is important
if reproducible results are to be obtained (Cantle, 1982).
On the other hand for the light source, the continuum or white light source is usually used
which emits in the visible and UV part of the spectrum. However, since the absorption
phenomenon being measured is occurring over an extremely narrow part of the spectrum (0.01
nm) it would require very high resolution to measure any significant absorption from a continuum
lamp. Other problems with using typical UV/Vis continuous light source include difficulty in seeing
the decrease in signal when atoms absorb in a small bandwidth, insignificant decrease in total
signal area, and resultant bad sensitivity with large amounts of elements. A line source differs
from a continuum source in that it emits only at discrete wavelengths. The spectral lines are
narrower than the absorption lines being measured, thus high resolution is not required (Cantle,
1982).
In AAS, there are some essential requirements in choosing light sources. It is important
that the light source emit a steady uniform level of radiation to obtain an analytical signal of low
noise level allowing good precision of measurement and good detection limits. In many cases it
is also important that the source should show little long-term drift in emission intensity. The
spectral lamp used should also require the minimum of maintenance and adjustment to obtain
optimum performance. The characteristics of the spectra produced by sharp line sources are also
important. They should be clean, with the minimum interference to the analytical line due to the
presence of spectral lines of filler gas or impurities in the lamp (Kirkbright and Sargent, 1974).
The most widely used line source for the production of fine line spectra is the hollow-
cathode lamp (HCL), which was introduced in 1916 by Paschen. It is highly advantageous due to
several factors: (1) spectral lines may be produced over the whole optical region from the infrared
to the vacuum ultraviolet; (2) intense spectra may be excited using only low currents; (3)
extremely sharp lines are quite readily obtained; (4) the lamps and their associated power
supplies are relatively simple and inexpensive to construct; and (5) they give stable, noise-free
output over long periods of time (Kirkbright and Sargent, 1974).
In general terms the hollow-cathode lamp consists of a sealed tube containing an anode,
a cylindrical hollow cathode made of, or sometimes lined with, the metal whose spectrum is
required, and an inert gas at a pressure of about 1 to 5 torr (depending on the gas used) .
When a potential of 500 to 1000 volts is applied between the electrodes a discharge of the carrier
gas strikes and appears as a glowing positive column. However, at the pressure used, the
discharge concentrates into the hollow cathode, giving higher current densities than those
normally attained in the discharge and hence intense illumination of the cathode space. As the
ions bombard the inner surface of the cathode, a strong sputtering action ensues as they displace
atoms form the surface. The sputtered metal atoms accumulate inside the cathode and are
excited to give an emission spectrum by second-order collisions with the high concentration of
excited inert gas atoms and ions. The sputtering mechanism of the HCL explains its ability to
produce intense metal spectra even when cooled to temperatures at which the vapor pressure of
the metal is negligibly small. However, use of hollow-cathode lamps can be expensive, since
different lamps are to be used for each element being tested (Kirkbright and Sargent, 1974).
Monochromators in AAS select transmitted light from the element of interest, and not from
the incident light. They separate, isolate, and control the intensity of radiant energy reaching the
detector. Specifically, it selects a specific, narrow region of the spectrum for transmission to the
detector and rejects all wavelengths outside this region. Ideally, the monochromator should be
capable of isolating the resonance line only and excluding all other wavelengths. Several methods
may be used to isolate the required part of the spectrum, but the diffraction grating
monochromator is now universally used in atomic absorption instruments. A schematic of a
diffraction grating monochromator is shown in Figure 2.10.
The radiation from the hollow-cathode lamp enters the monochromator through the entrance slit
and is focused onto the grating. The grating disperses the radiation into individual wavelengths.
By rotating the grating, the analytical wavelength of interest will pass through the exit slit and be
focused on the detector (Cantle, 1982).
Detectors for AAS/AFS do not employ photographic technique, for such is inconvenient for
measuring fluorescence signals for analytical use and would be quite impracticable for routine
absorption analysis. AAS detectors are able to convert the light signal into an electrical signal for
direct reading. Such devices must be sufficiently compact for use in the optical systems of small,
modern spectrometers, give sufficient sensitivity to produce stable and noise-free signals, have a
reasonably uniform spectral response over the wavelength range of interest and be simple to use
and give reliable operation over long periods (Kirkbright and Sargent, 1974).
Solid state photocells are the simplest of the photoelectric radiation detectors and are
widely used for light-metering applications. The mechanism of their photoconductivity is
synonymous to that of semi-conductors – the electrons may be categorized according to the band
of energy values in which they lie. When energy is supplied to the material, some electrons in the
valence band are excited into the conduction band. As the electrons in the conduction band are
more mobile this process increases the conductivity of the material. On the other hand, photo-
emissive cells depend upon the fact that when many metals are irradiated by photons of sufficient
energy, electrons are emitted from the surface of the metal (Kirkbright and Sargent, 1974).
The most commonly used detector for AAS are photomultiplier tubes. These devices
represent a logical development of the photo-emissive cell. Photons from the radiation source
bombard a cathode containing a photo-emissive substance. This causes electrons to be dislodged
from the cathode which then travel to the anode. The photomultiplier consists of a photo-emissive
cathode and an anode to collect the displaced electrons. Between the cathode and anode are
additional photo-emissive plates called dynodes. Each dynode collects electrons from the cathode
or previous dynode. The bombarding electrons dislodge several electrons from the next dynode
producing an extremely high flow of electrons at the anode. The operator controls the voltage
between anode and cathode and thus sets the ‘gain’ of the detector. This voltage will vary from
about 200-1000 volts to produce a wide range of gain settings. Since random processes within
the tube will be expanded also, the lowest voltage that is practical should always be used to avoid
excessive noise (Cantle, 1982).
Figure 2.11. Schematic representation of a photomultiplier tube.
(Diagram obtained from Kirkbright, G.F. and Sargent, M. 1974. Atomic Absorption
and Fluorescence Spectroscopy. London: Academic Press Inc. Ltd, p. 394)
The readout system in AAS presents the results from the spectroscopic process digitally.
This avoids errors in scale readings on meters trough parallax, misinterpolation between scale
divisions etc. It also consists of a logarithmic amplifier, which converts the amplified output from
the photomultiplier into read in absorbance units, the logarithmic function of percent absorption.
The scale expansion control of the spectrometer can continuously expand very small signals. This
facilitates the reading of small absorbances but it should also be accounted that any fluctuations
in the signal will be scale-expanded as well. It is not always immediately grasped that readout
directly in concentration units is achieved via the scale expand function (Cantle, 1982).
Limits of detection for flame atomic absorption spectroscopy (FAAS) are typically in the
range 0.01 to 0.1 μg mL-1. The linear dynamic range i.e, the maximum range over which the
calibration curve is linear, is limited by the propensity for self-absorption in the flame, and is
generally more than three orders of magnitude. Errors in AAS are usually minimized by the use
of a line source and the ratio method (i.e., ratio of incident to transmitted light, I0/I). Thus, if the
wavelength setting is seriously incorrect, it is unlikely that any absorption will be observed. If the
wavelength is incorrectly tuned, the effects on the value of I will roughly equal those on the value
of I0, and the error may not be too serious. A stable uptake rate or aspiration rate is required to
be taken in AAS in order for the ratio method to take effect, for such method cancels out many
instrumental errors such as long-term source drift and small monochromator drifts. The said rate
declines as the viscosity of the solution sprayed is increased. Nebulizer uptake interferences can
be minimized if the dissolved salt content of samples and standards is approximately matched
(Ebdon, et. al., 1998).
Analyte or multiplicative interferences change the magnitude of the analyte signal itself.
They can either be physical or chemical in nature. Physical interferences can alter the aspiration,
nebulization, desolvation, or volatilization processes. Substances in the sample that change the
solution viscosity, for example, can alter the flow rate and the efficiency of the nebulization
process. Combustible constituents, such as organic solvents, can change the atomizer
temperature and thus affect the atomization efficiency indirectly (Skoog, et. al., 2014).
Chemical interferences are usually specific to particular analytes. They occur in the
conversion of the solid or molten particle after desolvation into free atoms or elementary ions.
One cause of such interference is the formation of compounds of low volatility. The best known
of this type of interference is that of phosphate (also sulfate and silicate) on calcium, in which
the signal produced by calcium through AAS is decreased due to the decrease in the amount of
analyte atomized. This interference can be avoided by increasing the temperature of the flame
to enhance atom production, adjust the nebulizer to produce a smaller particle size, and make
observations higher in the flame. Moreover, a releasing agent, or an element that will enter into
a law of mass action competition with the analyte to combine with the interferent, is used. If an
excess of the releasing agent is added, the analyte is released from the interfering anion. Another
solution is to use a protective chelating agent which preferentially complexes the analyte,
protecting it from the grasp of the interferent. For example, excess EDTA protects calcium from
phosphate interference by binding to Ca2+ (Ebdon, et. al., 1998).
The formation of analyte oxides and hydroxides may also cause chemical interference.
Such compounds are non-volatile and are capable of absorbing intense amount of light. This can
be avoided by increasing the flame temperature and using less concentration of the oxidant.
Another chemical interferences are ionization interferences, also called vapor-phase interferences
or cation enhancement. A demonstration of this type is the doubling of the intensity of rubidium
absorption by the addition of potassium, in the air-acetylene flame. This is caused by ionization
suppression, and if uncorrected will lead to substantial positive errors when the samples contain
easily ionized elements and the standards do not. The problem is easily overcome by adding an
ionization suppressant (or buffer) in large amount to all samples and standards. The suppressant
creates high concentration of electrons, which suppresses the metal cation or analyte by shifting
equilibrium (Ebdon, et. al., 1998).
Ca Analysis
0.3
0.15
0.1
0.05
0
0 2 4 6 8 10
Concn, ppm
Table 2.3. Data on the concentration of calcium in the Bear Brand samples.
Brand Trial Concentration Average
BB Adult 1 227.9611586 250.9279512
2 273.8947438
BB Kids 1 255.1037317 215.9557898
2 176.8078478
BB Strawberry 1 146.5168688 144.820458
2 143.1240472
As shown in Figure 2.12, the standard curve shows a very linear relationship between the
calcium concentration and the absorbance. The calculated values for the R2, the slope (m) and
the y-intercept are 0.99971, 0.02993 and 0.0020, respectively. This R2 value implies that the
relationship between the calcium concentration of the standards and the absorbance for the
standard is linear enough to be utilized as a curve. The equation of the line was obtained as y =
0.02993x + 0.00200.
On the other hand, as shown in Table 2.3., it can be seen that the average calcium
concentration of BB adult is 250.93 ppm. Meanwhile, the average calcium concentration of BB
kids is 215.96 ppm, and for the strawberry is around 144.82 ppm. Among the brands, the BB adult
sample has the highest concentration of calcium followed by BB kids and the BB strawberry. ADD
MORE
For the sodium concentration determination, its concentration was determined in BB kids.
similar procedures as that for calcium analysis were done except that 5% Cs+ was added to each
working standard instead of La3+ before diluting to 50 mL. The solutions were also placed in plastic
containers. The table below shows the data collected.
In this process, sodium is atomized and ionized in the air-acetylene flame. Thus, cesium
is added to suppress the ionization effect since cesium can be easily ionized; this is done most
effectively by adding about 0.5 g/L cesium (Cs). Although on the other hand, cooler flame from
air-propane or air-hydrogen flame can be used to avoid ionization (Welz and Sperling, 1999).
Table 2.3. Data on the absorbance of sodium standards with Cs2+ and the milk
samples.
Concentration, ppm A A (Corrected)
0 0.0062 0
2 0.0373 0.0311
4 0.0598 0.0536
6 0.0836 0.0774
8 0.1152 0.109
10 0.148 0.1418
Method Blank 0 0
BB kids 1 0.1025
BB kids 2 0.0892
Na Analysis
0.15
y = 0.0138x - 0.0002
Absorbance
0.1 R² = 0.9955
0.05
0
0 2 4 6 8 10 12
-0.05
Concn, ppm
As shown in Figure 2.13, the standard curve shows a very linear relationship between the
lead concentration and the absorbance. The calculated values for the R2, the slope (m) and the
y-intercept are 0.9955, 0.0138 and -0.0002, respectively. This R2 value implies that the
relationship between the sodium concentration of the standards and the absorbance for the
standard is linear enough to be utilized as a curve. The equation of the line was obtained as y =
0.0138x - 0.0002.
On the other hand, it can be seen in Table 2.4. that the average concentration of sodium
in the BB kids sample is 347.90 ppm. ADD MORE
Lastly, lead traces (Pb) in a given water sample was analyzed. Lead is is the most common
element determined in AAS. The metal can be determined in the air-acetylene flame. In addition,
it can be determined accurately due to lack of interferences in the process although some
interferences such as aluminium or iron can be easily be removed through the addition of ascorbic
acid, citric acid, and EDTA (Welz and Sperling, 1999).
External calibration and standard addition were used in the determination for lead. For
external calibration, similar to the previous analyses, different concentrations of Pb standards
were prepared without the inclusion of any additive. The table below shows the collected data for
the external standard determination of lead traces.
Table 2.5. Data on the absorbance of lead standards with and the unknown water
samples.
Concentration, ppm A A (Corrected)
0 0.0027 0
2 0.0378 0.0351
4 0.072 0.0693
6 0.1007 0.098
8 0.1365 0.1338
10 0.1617 0.159
Method Blank 0 0
Unknown T1 0.0434
Unknown T2 0.0434
Pb Analysis
0.18
0.16 y = 0.016x + 0.0025
R² = 0.9979
0.14
0.12
Absorbance
0.1
0.08
0.06
0.04
0.02
0
0 2 4 6 8 10 12
Concn, ppm
Table 2.6. Data on the concentration of lead in the unknown water sample using external
calibration.
Brand Trial Concentration Average
Unknown 1 2.553729833 2.553729833
2 2.553729833
As shown in Figure 2.14, the standard curve shows a very linear relationship between the
lead concentration and the absorbance. The calculated values for the R2, the slope (m) and the
y-intercept are 0.9979, 0.016 and 0.0025, respectively. This R2 value implies that the relationship
between the lead concentration of the standards and the absorbance for the standard is linear
enough to be utilized as a curve. The equation of the line was obtained as y = 0.016x + 0.0025.
On the other hand, it can be seen in Table 2.4. that the average concentration of lead in
the unknown sample is 2.55 ppm. ADD MORE
Meanwhile, for the second method, standard addition was used to determine the traces of
lead in the unknown sample. A known amount of the sample being determined is added to the
unknown sample prior to the analysis of total amount of constituent present. It is done to minimize
systematic errors. The concentration of the unknown amount can then be determined by
interpolating the data of the volume used vis-à-vis and the absorbance of the known added
constituent (Jeffery, et. al., 1989).
The following equation shows the equation for the standard addition:
(1)
CA is the analyte concentration; b and m are the y-intercept and slope from the standard curve;
CS is the concentration of the spike or a solution of the analyte with known quantity; and VS is the
volume of the spike added. Table below shows the data for the standard addition determination
of the lead concentration. The standard of 6 ppm lead was used in the standard addition.
Table 2.7. Data on the analysis of Pb in water sample by standard addition technique.
0 0.0119
2 0.0181
4 0.0228
6 0.0287
8 0.0332
0.025
0.02
0.015
0.01
0.005
0
0 2 4 6 8 10
Volume Added
Figure 2.15. The standard curve for the standard addition of lead.
Table 2.8. Data on the concentration of lead in the unknown water sample using standard
addition
As shown in Figure 2.15, the standard curve shows a linear relationship between the
volume added and the absorbance. The calculated values for the R2, the slope (m) and the y-
intercept are 0.9974, 0.0027 and 0.0123, respectively. This R2 value implies that the relationship
between the lead concentration of the standards and the absorbance for the standard is linear
enough to be utilized as a curve. The equation of the line was obtained as y = 0.0027x + 0.0123.
Meanwhile, as shown in table 2.8., the determined concentration of the lead sample by using
standard addition is 2.77 ppm.
Table 2.9. The comparison between the two methods used in the determination of lead
concentration.
Method Average concentration Percent error
External Calibration 2.553729833 2.149193
Standard Addition 2.77443609 10.97744
The theoretical value for the unknown sample is 2.5 ppm. As shown in table 2.9., the
external calibration method showed a percent error of 2.14%. Meanwhile, the standard addition
methods showed a percent error of 10.97%. The external calibration method showed a higher
accuracy in comparison with the standard addition method. ADD MORE
The possible sources of errors in the experiment are the improper dilution during the
preparation of the standards; improper ignition and charring of the milk samples leading to a lower
concentration value; and the non-addition of the working standards.
IV. Sample Calculations
𝑦−𝑏
[𝐶𝑎] 𝑇𝑟𝑖𝑎𝑙 1 =
𝑚
0.1337 − (0.0027)
[𝐶𝑎] 𝑇𝑟𝑖𝑎𝑙 1 = 𝑥50
0.0287 ppm−1
[𝐶𝑎] 𝑇𝑟𝑖𝑎𝑙 1 = 227.9611586 ppm
𝑧−𝑏
[𝐶𝑎] 𝑇𝑟𝑖𝑎𝑙 2 =
𝑚
0.1601 − (0.0027)
[𝐶𝑎] 𝑇𝑟𝑖𝑎𝑙 2 =
0.0287 ppm−1
[𝐶𝑎] 𝑇𝑟𝑖𝑎𝑙 2 = 273.8947438 ppm
𝑦−𝑏
[𝑁𝑎] 𝑇𝑟𝑖𝑎𝑙 1 =
𝑚
0.1025 − (− 0.0002)
[𝑁𝑎] 𝑇𝑟𝑖𝑎𝑙 1 = 𝑥50
0.0138ppm−1
[𝑁𝑎] 𝑇𝑟𝑖𝑎𝑙 1 = 371.9779272 ppm
𝑧−𝑏
[𝑁𝑎] 𝑇𝑟𝑖𝑎𝑙 2 =
𝑚
0.0892 − (− 0.0002)
[𝑁𝑎] 𝑇𝑟𝑖𝑎𝑙 2 = 𝑥50
0.0138 ppm−1
[𝑁𝑎] 𝑇𝑟𝑖𝑎𝑙 2 = 323.8144508 ppm
𝑦−𝑏
[𝑃𝑏] 𝑇𝑟𝑖𝑎𝑙 1 =
𝑚
0.0434 − 0.0025
[𝑃𝑏] 𝑇𝑟𝑖𝑎𝑙 1 =
0.016 ppm−1
[𝑃𝑏] 𝑇𝑟𝑖𝑎𝑙 1 = 2.553729833 ppm
𝑦−𝑏
[𝑃𝑏] 𝑇𝑟𝑖𝑎𝑙 2 =
𝑚
0.0434 − 0.0025
[𝑃𝑏] 𝑇𝑟𝑖𝑎𝑙 2 =
0.016 ppm−1
[𝑃𝑏] 𝑇𝑟𝑖𝑎𝑙 2 = 2.553729833 ppm
[𝑃𝑏] 𝑇𝑟𝑖𝑎𝑙 1 + [𝑃𝑏] 𝑇𝑟𝑖𝑎𝑙 2
Average concentration of Pb in sample =
2
2.553729833 + 2.553729833
Average concentration of Pb in sample =
2
Average concentration of Pb in sample = 2.553729833ppm
x-intercept = VS:
𝑘𝐶
−( 𝑉 𝐴 )
𝑓
𝑉𝑆 =
𝑘𝐶
( 𝑉 𝑆)
𝑓
Definition of variables:
CA = concentration of analyte
CS = concentration of spike or standard
V0 = volume of sample
Vs = volume of spike or standard added
Sspike = spike or standard signal
CPb = concentration of Pb
CS = concentration of standard used
Vsx = volume of sample used
(𝑏)(𝐶𝑆 )
𝐶𝑃𝑏 =
(𝑚)(𝑉0 )
(0.0123)(6 𝑝𝑝𝑚)
𝐶𝑃𝑏 =
(0.0027 𝑚𝐿−1 )(10 𝑚𝐿)
𝐶𝑃𝑏 = 2.77443609 ppm
Aulisa, E. and Gilliam, D. 2015. A Practical Guide to Geometric Regulation for Distributed
Parameter Systems. United States: CRC Press, p. 459.
Cantle, J.E. 1982. Atomic Absorption Spectrometry. New York: Elsevier Science
Publishing Company Inc., pp. 15-18, 30-31.
Ebdon, L., Evans, E.H., Fisher, A.S., and Hill, S.J. 1998. An Introduction to Analytical
Atomic Spectrometry New York: John Wiley & Sons, pp. 1-2.
Jeffery, G.H., Bassett, J., Mendham, J. and Denney, R.C. 1989. Vogel’s Textbook of
Quantitative Chemical Analysis. Great Britain: Longma Group UK Limited.
Kirkbright, G.F. and Sargent, M. 1974. Atomic Absorption and Fluorescence Spectroscopy.
London: Academic Press Inc. Ltd, pp. 4-5, 99-113, 394.
Michael Hollas, J. 2004. Modern Spectroscopy, 4th ed. England: John Wiley & Sons, Ltd.
Skoog, D.A., West, D.M., James Holler, W.F., and Crouch, S.R. 2014. Fundamentals of
Analytical Chemistry, 9th ed. United States: Brooks/Cole, pp. 773-777.
Van Loon, J.C. 1980. Analytical Atomic Absorption Spectroscopy: Selected Methods. New
York: Academic Press, Inc., p. 1.
Welz, B. and Sperling, M. 1999. Atomic Absorption Spectrometry, 3rd ed. Germany:
Wiley-VCH, pp. 1, 493-494.