Académique Documents
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Culture Documents
and Serology
Hawler Medical University
College of Health Sciences
Clinical Biochemistry Dept.
Ass. Lec. Amer Ali Khaleel
(M.Sc. Medical Immunology)
Lab.7
Helicobacter pylori
Introduction:
• Helicobacter pylori is major human pathogen associated with gastric
antral epithelium in patients with active chronic gastritis.
• Produces inflammatory
response.
Time
No. Methods
Consuming
Serology level
1 H. pylori Antibody Test Cassettes or Strips by Immunochromatography assay 10-15 min
2 H. pylori Antigen Stool (fecal) Strips test by chromatographic Immunoassay 10-15 min
Molecular level
6 PCR (Polymerase Chain Reaction) 7-10 days
1-H. pylori antibody test cassette or strips by ICA.
• Widely available.
• Positive result may reflect previous (old) rather than current (recent)
infection not recommended for confirming eradication.
• Blood tests for H. pylori cannot tell if you have a current infection or
how long you have had it, This is because the test can be positive for
years even if the infection is cured.
1-H. pylori antibody test cassette or strips by ICA.
• As a result, blood tests cannot be used to see if the infection has been
cured after treatment.
2- H. pylori antigen stool (fecal) stripes test ICA
• H. pylori Ag Rapid test kit result window has 2 pre-coated lines, "T"
(H. pylori Ag Test Line) and "C" (Control Line).
• Both the Test Line and the Control Line in result window are not visible
before applying any samples.
• The Control Line is used for procedural control and should always
appear if the test procedure is performed correctly.
exalenz.com
Biopsy:
Biopsy most accurate way to detection of H. pylori infection.
Confirmation Positive
of cure
Flow-chart
Medication
Eradication therapy
Safety Guidelines
1-You must wear a lab coat (and do it up) in all Immunology labs.
2-At the end of the lab. clean your lab bench and equipment.
Any Questions