1st Semester

M.BT-1.1 (Full marks-100)

Advanced bio-separation Technology

An overview of bioseparation. Separation of cells and other insolubles from fermented broth.
Filtration and microfiltration, centrifugation (batch, continuous, basket). Cell disruption: Physical
methods (osmotic shock, grinding with abrasives, solid shear, liquid shear), Chemical methods
(alkali, detergents), Enzymatic methods; Products isolation: Solvent Extraction and adsorption
method, precipitation (ammonium sulphate. Organic solvents, high molecular weight polymers),
chromatographic separation; affinity, size exclusion, Thin layer, ion exchange chromatography.
ultrafiltration, Reverse Osmosis, Electrophoretic separation ; Products polishing: Crystallization
and drying.

Texts/References
1. Michael Shuler and Fikret Kargi, Bioprocess Engineering: Basic
Concepts, 2nd Edition, Prentice Hall, Englewood Cliffs, NJ, 2002.
2. Pauline Doran, Bioprocess engineering principles, 1 Edition, Academic
Press, 1995.
3. Colin Ratledge, Bjorn Kristiansen, Basic Biotechnology, 2 Edition, nd

Cambridge University Press, 2001.

4. Roger Harrison et al., Bioseparations Science and Engineering, Oxford
University Press, 2003.

M.BT-1.2 (Full marks-100)

Immunotechnology

Characteristics of animal cells and their implication on process design, Nutritional

requirements and serum free culture of mammalian cells Kinetics of growth and product
formation. Reactor systems for large-scale production using animal cells. Production of
Ployclonal antibodies with different types of antigens : antigen preparation and
modification, adjuvants, dose and route of antigen administration, collection of sera,
purification of antibodies. Hybridoma technology - production and applications of
monoclonal antibodies for diagnosis and therapy. Production of virus vaccines, specific
vaccines. Production of cellular chemicals like Interferons, Interleukin etc. Immunoassay
procedures.

Texts/References:
1. Kuby, RA Goldsby, Thomas J. Kindt, Barbara, A. Osborne Immunology, 6 th Edition,
Freeman, 2002.
2. Brostoff J, Seaddin JK, Male D, Roitt IM., Clinical Immunology, 6 th Edition, Gower
Medical Publishing, 2002.
3. Janeway et al., Immunobiology, 4th Edition, Current Biology publications. 1999.
4. Paul, Fundamental of Immunology, 4th edition, Lippencott Raven, 1999.

M.BT-1.3 (Full marks-100)

Departmental Elective I

M.BT-1.4 (Full marks-100)

Departmental Elective II

M.BT-1.5 (Full marks-100)

Allied Departmental Elective III

M.BT-1.6 (Full marks-100)

Core Lab 1: Immunotechnology Lab

1. Selection of animals, Preparation of antigens, Immunization and

methods of bleeding, Serum separation, Storage.
2. Antibody titre by ELISA method.
3. Double diffusion, Immuno-electrophoresis and Radial
Immunodiffusion.
4. Complement fixation test.
5. Isolation and purification of IgG from serum or IgY from chicken egg.
6. SDS-PAGE, Immunoblotting, Dot blot assays
7. Blood smear identification of leucocytes by Giemsa stain
8. Separation of leucocytes by dextran method
9. Demonstration of Phagocytosis of latex beads
10. Separation of mononuclear cells by Ficoll-Hypaque
11. Flowcytometry, identification of T cells and their subsets
12. Lymphoproliferation by mitogen / antigen induced
13. Lympnode Immunohistochemistry (direct and indirect peroxidase
assay)
14. Hybridoma technology and monoclonal antibody production.
15. Immunodiagnostics using commercial kits

M.BT-1.7 (Full marks-100)

Core Lab II: Advanced Bioseparation Technology Lab

1Centrifugation in batch and continuous centrifuge

2. Adsorption process in batch and continuous mode.
3. Cell disruption and biomass recovery
4. Heavy metal precipitation reduction and recovery

Communicative English

2nd Semester
M.BT-2.1

Advanced genetic engineering (Full Marks-100)

Tools of recombinant DNA: restriction endonucleases and other enzymes, plasmid,

bacteriophage, cosmid and other vectors. c-DNA and genomic library, Gene isolation,
cloning and expression, DNA sequencing, oligonucleotide synthesis, Southern and
Northern hybridization, FISH, PCR, RAPD, RFLP, DNA fingerprinting and their
applications for diagnosis of disease, site-directed mutagenesis, Gene silencing, Gene
transfer technologies, Gene therapy; Molecular basis of genetic diseases, genetic
counseling. Functional genomics: DNA chips and microarray gene screen technology;
site directed mutagenesis, transgenic animals and gene knockout techniques, cell culture
based techniques.

Text/References:
1. S.B. Primrose, R.M. Twyman and R.W.Old; Principles of Gene
Manipulation. 6th Edition, S.B.University Press, 2001.M.Tech
(Biotechnology & Biochemical Engineering)
2. J. Sambrook and D.W. Russel; Molecular Cloning: A Laboratory
Manual, Vols 1-3, CSHL, 2001.
3. Brown TA, Genomes, 3rd ed. Garland Science 2006
4. Selected papers from scientific journals.
5. Technical Literature from Stratagene, Promega, Novagen, New
England Biolab etc.

M.BT-2.2
Biotechnology of Plant Metabolites (Full marks-100)

Introduction to aspects of plant tissue/cell culture e.g. nutrition and media, callus culture.
Cell suspension culture. Introduction to primary and secondary metabolism, Important
pathway leading to the biosynthesis of secondary metabolites (e.g. serpentine, shikonin,
diosgenin and cardenolids) in plants, Secondary. Metabolic products produced by in vitro
culturing of plant cells, selection of plant cells/tissues for the production of a specific
product, Molecular Pharming. Culture system in secondary plant product biosynthesis-
batch / continuous culture and immobilized plant cells. Biotransformation of precursors
by cell culturing. Extraction and analytical methods for the above four metabolites.
Industries involved in the production of plant secondary metabolites, Potential and future
prospect of the secondary metabolities production by plant cells culture techniques.

References:

1. S. NArayanswamy. Plant cell and tissue culture. Tata McGraw Hills.

2. Selected papers from scientific journals.
3. Technical Literature from Stratagene, Promega, Novagen,
NewEngland Biolab etc.

M.BT-2.3 (Full marks-100)

Departmental Elective I

M.BT-2.4 (Full marks-100)

Departmental Elective II

M.BT-2.5 (Full marks-100)

Allied Departmental Elective III

M.BT-2.6 (Full marks-100)

Core Lab 1: Plant biotechnology Lab

1. Callus culture
2. Suspension culture
3. In-vitro production of secondary metabolite
4. Extraction methods for bioactive compounds
5. Characterization of bioactive compound

M.BT-2.7 (Full marks-100)

Core Lab 1: Advanced genetic engineering Lab

1. Isolation and characterization of genomic DNA for E.Coli;

2. Unit determination of restriction enzyme activity;
3. Cutting of DNA and clean up of DNA for ligation;
4. Evaluation of transformants and preparation of glycerol stocks; Demonstration of
electorporation;
5. Preparation of radiolabelled DNA probe (random primer labeling); b.
Hybridization, washing & autoradiography (Cleaning and monitoring work bench
for radioactive spill);
6. Induction of Lac operon;
7. Demonstration of PCR; Setting up PCR reaction; b. Analysis of amplified
product;
8. Minipreparation & digestion of plasmid DNA
9. Southern transfer of plasmid DNA digest & baking of membrane
10. Phage unification, titration and preparation of stocks; b. Isolation of phage DNA
11. Demonstration of DNA sequencing; Setting up sequencing reactions; Casting
sequencing gel
12. Gel electrophoresis & autoradiography. d. Reading sequencing from X-ray film

3rd Semester
M.BT-3.1 (Full marks-100)

Advanced bioinformatics
Sequence databases; Similarity matrices; Pairwise alignment; BLAST;
Statistical significance of alignment; Sequence assembly; multiple
sequence alignment; Clustal; Phylogenetics: distance based approaches,
maximum parsimony. Motif representation: consensus, regular
expressions; PSSMs; Markov models; Regulatory sequence identification
using Meme; Gene finding: composition based finding, sequence motif-
based finding.Representation of molecular structures (DNA, mRNA,
protein), secondary structures, domains and motifs; Structure
classification (SCOP, CATH); Visualization software (Pymol, Rasmol etc.);
Structure databases; Secondary structure prediction; RNA structure
prediction; Mfold; Protein structure prediction by comparative modelling
approaches(homology modelling, threading); Ab initio structure
prediction: force fields, backbone conformer generation by Monte Carlo
approaches, side-chain packing; Energy minimization; Molecular
dynamics; Rosetta; Structure comparison (DALI, VAST etc.); CASP;
Protein-ligand docking; Computer-aided drug design (pharmacophore
identification); QSAR; Protein-Protein interactions

Labs in Advanced bioinformatics

1. BLAST search and alignment
2. Protein structure alignment using CLUSTAL W
3. Biomolecules structure conformation using RASMOL
4. Secondary structure predictions
5. Computer aided drug designing

Texts/References:
1. David W. Mount. Bioinformatics: Sequence and Genome Analysis
2nd Edition, CSHL Press, 2004.
2. Baxevanis and F. B. F. Ouellette, Bioinformatics: a practical guide
to the analysis of genes and proteins, 2nd Edition, John Wiley,
2001.
3. Jonathan Pevsner, Bioinformatics and Functional Genomics, 1 st

Edition, Wiley-Liss, 2003.

4. P. E. Bourne and H. Weissig. Structural Bioinformatics.
Wiley.2003.
5. C. Branden and J. Tooze, Introduction to Protein Structure,
2ndEdition, Garland Publishing, 1999.

M.BT-3.2 (Full marks-100)

Industrial biotechnology

Idea of fermentation, Cell growth, Regulation of metabolism, Substrate

assimilation/Product Secretion; Different fermentative system; Batch and continuous
Process, Fermentor Design ,Surface and submerged liquid substrate fermentation; Solid
substrate fermentations, Fermentation substrate raw materials,Bio-mass production
(Alcohol, lattice acid, cheese making, soya based foods, vinegar, industrial chemical, bio
polymer, bio insecticides, food additive [amino acids, nucleosides, vitamins, fats and
oils], health care products {antibiotics steroids, vaccines}, Production of industrial
solvents[alcohol, acetone butanol etc.]); Industrial Enzymes (amylase, proteases, lipases),
concepts of immobilized enzymes. Introduction to food technology, elementary idea of
canning and packing fat-based edible products, sterilization and pasteurization of food
products

References

1. General Microbiology, Stanier, et. al., 5th ed.

2. Industrial Microbiology, Casida
3. Industrial Microbiology, Prescott
4. Principles of fermentation technology, P F Stanbury and A Whitaker, Pergamon
press (1984)

M.BT-3.3 (Full marks-100)

Departmental Elective I

M.BT-3.4 (Full marks-100)

Seminar

M.BT-3.4 (Full marks-100)

Project I

4th Semester

M.BT-4.1 (Full marks-100)

Project II

List of Departmental electives

Biological waste treatment

Advanced cell and molecular biology

Food biotechnology

Protein engineering

Biophysics and structural biology

List of allied departmental electives

Nano-biotechnology

Computational biotechnology

Industrial pharmacology