2100 Expert - DNA 1000 - DE72901983 - 2010-08-16 - 15-47-55 (Testing AMPure)

2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.
xad Page 1 of 14
Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

Data Path: C:\...-16\2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad Modified: 8/16/2010 4:41:57 PM
Electropherogram Summary
Ladder
Peak table for Ladder

Pea Size Conc. Molarity Observati Area Aligned Migration Peak Peak % of Time corrected
k [bp] [ng/µl] [nmol/l] ons Time [s] Height Width Total area
1 15 4.20 424.2 Lower 25.0 43.00 100.4 2.4 0.0 25.0

Marker
2 25 4.00 242.4 Ladder 26.2 45.22 99.0 2.6 0.0 26.2
Peak
3 50 4.00 121.2 Ladder 34.4 47.50 114.3 1.7 0.0 34.4
Peak
4 100 4.00 60.6 Ladder 41.2 53.93 149.1 2.1 0.0 41.2
Peak
5 150 4.00 40.4 Ladder 45.0 59.87 261.6 2.1 0.0 45.0
Peak
6 200 4.00 30.3 Ladder 52.0 65.53 289.4 2.1 0.0 52.0
Peak
7 300 4.00 20.2 Ladder 63.4 76.84 316.1 2.6 0.0 63.4
Peak
8 400 4.00 15.2 Ladder 76.2 86.56 320.9 2.9 0.0 76.2
Peak
9 500 4.00 12.1 Ladder 82.9 93.37 323.6 3.1 0.0 82.9
Peak
10 700 4.00 8.7 Ladder 87.7 103.48 300.3 2.6 0.0 87.7
Peak
11 850 4.00 7.1 Ladder 86.1 106.57 318.7 1.8 0.0 86.1
Peak
12 1,000 4.00 6.1 Ladder 89.8 108.21 306.8 2.6 0.0 89.8
Peak
13 1,500 2.10 2.1 Upper 52.0 113.00 154.6 2.6 0.0 52.0
Marker
2100 Expert (B.02.07.SI532) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 8/16/2010 4:43:14 PM
2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.xad Page 2 of 14

Electropherogram Summary Continued ...
Preclearing - 25bp Ladder (1)
Overall Results for sample 1 : Preclearing - 25bp Ladder (1)

Number of peaks found: 11
Peak table for sample 1 : Preclearing - 25bp Ladder (1)

Pea Size Conc. Molarity Observati Area Aligned Migration Peak Peak % of Time
k [bp] [ng/µl] [nmol/l] ons Time [s] Height Width Total corrected area
1 15 4.20 424.2 Lower 55.3 43.00 86.7 2.6 0.0 140.3

Marker
2 374 0.40 1.6 14.0 84.05 29.0 0.6 0.7 15.4
3 489 1.43 4.4 56.7 92.64 110.8 0.8 2.9 55.7
4 555 1.33 3.6 54.1 96.18 68.3 1.6 2.8 50.9
5 633 2.69 6.4 112.3 100.11 136.6 1.1 5.8 100.9
6 652 1.93 4.5 80.8 101.07 130.7 0.8 4.2 71.9
7 675 7.80 17.5 329.0 102.22 352.8 1.4 17.0 288.8
8 780 7.51 14.6 315.9 105.13 436.3 1.2 16.3 268.7
9 838 6.47 11.7 270.2 106.32 312.2 1.0 14.0 227.0
10 910 5.40 9.0 228.7 107.23 253.9 1.2 11.8 190.2
11 1,076 7.14 10.1 315.2 108.94 218.3 2.2 16.3 257.6
12 1,292 3.45 4.0 158.9 111.01 146.6 1.9 8.2 127.2
13 1,500 2.10 2.1 Upper 100.6 113.00 103.4 1.6 0.0 79.0
Marker

Preclearing - 25bp Ladder (2)
Overall Results for sample 2 : Preclearing - 25bp Ladder (2)

Peak table for sample 2 : Preclearing - 25bp Ladder (2)

1 15 4.20 424.2 Lower 59.1 43.00 90.6 2.7 0.0 150.3

Marker
2 369 0.47 1.9 16.6 83.53 30.6 0.8 0.7 18.5
3 374 0.79 3.2 28.2 84.01 42.5 1.2 1.1 31.2
4 489 2.63 8.2 105.9 92.66 150.4 2.3 4.3 104.7
5 556 1.20 3.3 49.5 96.19 65.6 2.1 2.0 46.9
6 635 5.05 12.1 212.7 100.17 200.4 1.7 8.6 192.5
7 651 3.93 9.1 166.3 101.02 202.9 1.0 6.7 149.1
8 676 11.19 25.1 476.8 102.26 441.4 2.5 19.3 421.8
9 782 10.66 20.7 452.8 105.16 534.1 1.6 18.3 388.2
10 842 6.29 11.3 265.3 106.41 314.1 1.0 10.7 224.4
11 908 5.20 8.7 222.7 107.21 246.2 1.1 9.0 186.8
12 1,063 7.20 10.3 320.2 108.82 212.3 2.2 13.0 264.2
13 1,290 3.28 3.9 152.8 110.99 144.0 1.8 6.3 123.3
14 1,500 2.10 2.1 Upper 101.7 113.00 102.4 1.9 0.0 80.5
Marker

Preclearing - 1Kb Ladder (1)
Overall Results for sample 3 : Preclearing - 1Kb Ladder (1)

Peak table for sample 3 : Preclearing - 1Kb Ladder (1)

1 15 4.20 424.2 Lower 56.4 43.00 92.2 2.0 0.0 144.4

Marker
2 454 0.09 0.3 13.9 90.24 22.3 1.3 13.2 15.4
3 480 0.01 0.0 2.5 91.99 3.1 1.8 2.4 2.7
4 906 0.50 0.8 88.8 107.18 115.0 2.5 84.4 81.8
5 1,500 2.10 2.1 Upper 423.2 113.00 490.2 2.6 0.0 368.5
Marker

Preclearing - 1Kb Ladder (2)
Overall Results for sample 4 : Preclearing - 1Kb Ladder (2)

Peak table for sample 4 : Preclearing - 1Kb Ladder (2)

1 15 4.20 424.2 Lower 55.8 43.00 91.7 1.9 0.0 144.7

Marker
2 455 0.08 0.3 12.4 90.33 20.4 1.3 12.2 14.0
3 481 0.00 0.0 0.0 92.07 -0.4 1.9 0.0 0.0
4 908 0.51 0.9 89.4 107.20 117.3 2.7 87.8 83.8
5 1,500 2.10 2.1 Upper 416.1 113.00 499.7 3.2 0.0 368.9
Marker

2nd Binding Sup - 25bp Ladder (1)

2nd Binding Sup - 25bp Ladder (2)

2nd Binding Sup - 1Kb Ladder (1)

2nd Binding Sup - 1Kb Ladder (2)

Elute (End-repaired DNA) - 25bp Ladder (1)
Overall Results for sample 9 : Elute (End-repaired DNA) - 25bp Ladder (1)
Peak table for sample 9 : Elute (End-repaired DNA) - 25bp Ladder (1)
1 15 4.20 424.2 Lower 64.8 43.00 108.4 2.4 0.0 171.8

Marker
2 250 1.71 10.4 54.3 71.24 80.7 2.1 73.2 84.6
3 505 0.31 0.9 14.1 93.64 13.2 2.5 19.0 16.6
4 734 0.10 0.2 4.7 104.18 7.9 1.7 6.3 5.0
5 1,042 0.02 0.0 1.1 108.61 3.0 2.1 1.5 1.1
6 1,500 2.10 2.1 Upper 114.2 113.00 131.3 5.9 0.0 110.7
Marker
7 1,807 0.00 0.0 17.7 115.94 21.8 2.0 0.0 16.7
8 2,198 0.00 0.0 38.5 119.68 18.7 12.3 0.0 35.2

Elute (End-repaired DNA) - 25bp Ladder (2)
Overall Results for sample 10 : Elute (End-repaired DNA) - 25bp Ladder (2)
Peak table for sample 10 : Elute (End-repaired DNA) - 25bp Ladder (2)
1 15 4.20 424.2 Lower 66.2 43.00 109.2 2.6 0.0 174.5

Marker
2 249 1.80 11.0 62.7 71.06 93.6 1.8 100.0 97.2
3 1,500 2.10 2.1 Upper 125.6 113.00 147.9 2.4 0.0 120.4
Marker
4 1,814 0.00 0.0 25.3 116.01 27.5 2.2 0.0 23.6
5 2,200 0.00 0.0 22.8 119.70 26.6 1.7 0.0 20.6

Elute (End-repaired DNA) - 1Kb Ladder (1)
Overall Results for sample 11 : Elute (End-repaired DNA) - 1Kb Ladder (1)
Peak table for sample 11 : Elute (End-repaired DNA) - 1Kb Ladder (1)
1 15 4.20 424.2 Lower 69.2 43.00 111.9 2.3 0.0 180.8

Marker
2 179 0.02 0.2 0.6 63.16 2.6 2.1 1.0 1.0
3 205 0.06 0.4 1.8 66.09 4.4 1.6 3.1 3.0
4 230 0.09 0.6 2.9 68.96 6.2 1.6 4.9 4.6
5 256 0.09 0.6 3.3 71.84 6.8 1.4 5.5 4.9
6 281 0.13 0.7 4.7 74.66 9.0 2.1 8.0 6.9
7 305 0.04 0.2 1.7 77.37 7.1 0.7 2.8 2.3
8 332 0.09 0.4 3.7 79.93 7.2 1.4 6.2 5.0
9 358 0.08 0.3 3.4 82.44 6.2 1.5 5.7 4.4
10 382 0.10 0.4 4.4 84.79 7.9 1.6 7.5 5.7
11 406 0.09 0.3 4.0 86.99 7.2 1.4 6.7 5.0
12 465 0.08 0.3 4.0 91.01 6.3 2.1 6.8 4.8
13 520 0.45 1.3 22.2 94.40 32.1 1.8 37.4 25.4
14 556 0.05 0.1 2.7 96.18 3.1 1.7 4.4 3.0
15 1,500 2.10 2.1 Upper 123.9 113.00 148.1 3.3 0.0 117.6
Marker

Elute (End-repaired DNA) - 1Kb Ladder (2)
Overall Results for sample 12 : Elute (End-repaired DNA) - 1Kb Ladder (2)
Peak table for sample 12 : Elute (End-repaired DNA) - 1Kb Ladder (2)
1 15 4.20 424.2 Lower 71.6 43.00 114.2 2.7 0.0 185.2

Marker
2 179 0.03 0.2 1.0 63.15 2.9 0.6 1.4 1.8
3 205 0.01 0.1 0.6 66.06 3.9 0.2 0.8 0.9
4 230 0.06 0.4 2.6 68.96 6.0 1.0 3.6 4.1
5 256 0.07 0.4 3.1 71.82 7.0 1.2 4.4 4.6
6 280 0.04 0.2 1.9 74.62 8.3 0.3 2.6 2.7
7 304 0.07 0.4 3.4 77.27 7.4 1.2 4.8 4.8
8 331 0.08 0.3 3.8 79.87 7.5 1.4 5.4 5.2
9 357 0.07 0.3 3.8 82.41 7.3 1.5 5.3 4.9
10 381 0.09 0.4 5.1 84.70 9.3 1.0 7.2 6.5
11 406 0.07 0.3 3.9 86.93 6.9 1.4 5.4 4.8
12 436 0.08 0.3 4.7 89.01 7.4 1.7 6.7 5.7
13 465 0.07 0.2 4.3 90.98 7.2 1.4 6.0 5.1
14 519 0.45 1.3 28.2 94.36 41.8 1.8 39.7 32.0
15 554 0.07 0.2 4.6 96.12 4.7 2.0 6.7 5.1
16 1,500 2.10 2.1 Upper 155.1 113.00 186.4 3.1 0.0 146.3
Marker
17 2,162 0.00 0.0 11.4 119.34 13.3 2.5 0.0 10.2

Gel Image

2100 Expert - DNA 1000 - DE72901983 - 2010-08-16 - 15-47-55 (Testing AMPure)

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2100 Expert - DNA 1000 - DE72901983 - 2010-08-16 - 15-47-55 (Testing AMPure)

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2100 expert_DNA 1000_DE72901983_2010-08-16_15-47-55.

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

Peak table for Ladder

1 15 4.20 424.2 Lower 25.0 43.00 100.4 2.4 0.0 25.0

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

Preclearing - 25bp Ladder (1)

Overall Results for sample 1 : Preclearing - 25bp Ladder (1)

Peak table for sample 1 : Preclearing - 25bp Ladder (1)

1 15 4.20 424.2 Lower 55.3 43.00 86.7 2.6 0.0 140.3

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

Preclearing - 25bp Ladder (2)

Overall Results for sample 2 : Preclearing - 25bp Ladder (2)

Peak table for sample 2 : Preclearing - 25bp Ladder (2)

1 15 4.20 424.2 Lower 59.1 43.00 90.6 2.7 0.0 150.3

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

Preclearing - 1Kb Ladder (1)

Overall Results for sample 3 : Preclearing - 1Kb Ladder (1)

Peak table for sample 3 : Preclearing - 1Kb Ladder (1)

1 15 4.20 424.2 Lower 56.4 43.00 92.2 2.0 0.0 144.4

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

Preclearing - 1Kb Ladder (2)

Overall Results for sample 4 : Preclearing - 1Kb Ladder (2)

Peak table for sample 4 : Preclearing - 1Kb Ladder (2)

1 15 4.20 424.2 Lower 55.8 43.00 91.7 1.9 0.0 144.7

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

2nd Binding Sup - 25bp Ladder (1)

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

2nd Binding Sup - 25bp Ladder (2)

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

2nd Binding Sup - 1Kb Ladder (1)

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

2nd Binding Sup - 1Kb Ladder (2)

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

Elute (End-repaired DNA) - 25bp Ladder (1)

1 15 4.20 424.2 Lower 64.8 43.00 108.4 2.4 0.0 171.8

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

Elute (End-repaired DNA) - 25bp Ladder (2)

1 15 4.20 424.2 Lower 66.2 43.00 109.2 2.6 0.0 174.5

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

Elute (End-repaired DNA) - 1Kb Ladder (1)

1 15 4.20 424.2 Lower 69.2 43.00 111.9 2.3 0.0 180.8

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

Elute (End-repaired DNA) - 1Kb Ladder (2)

1 15 4.20 424.2 Lower 71.6 43.00 114.2 2.7 0.0 185.2

Assay Class: DNA 1000 Created: 8/16/2010 3:47:55 PM

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