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Axioskop
Operating Manual
Copyright 3D Microscopy
Knowledge of this manual is required for the operation of the instrument. Would you therefore
please make yourself familiar with the contents of this manual and pay special attention to hints
concerning the safe operation of the instrument.
The specifications are subject to change.
Unless expressly authorized, forwarding and duplication of this document, and the utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
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3D Microscopy Contents
Contents
EC Declaration of Conformity
Annex (optional)
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Notes on safety 3D Microscopy
1 Notes on safety
1.1 General
Please make yourself familiar with the contents Caution!
of these operating instructions before Non-observance of the safety notes constitutes
attaching and starting up the components for a hazard for the user.
3D microscopy. Additional information is
available from our maintenance service or from
authorized agencies. Caution!
Non-observance of the safety notes constitutes
For the function of 3D microscopy in
a hazard for the instrument.
combination with the
Axiotron 2
Axiotech 100 Caution!
Axiotech 100 vario Disconnect the instrument from the line before
Axiovert 100/135/135 M opening it.
Axiovert 25 CA ☞ Note!
Axioplan 2/Axiophot 2 Notes which must be observed.
Axioskop
Axioskop 2
1.2 Regulations concerning
please see the operating instructions of these instrument safety and
microscopes.
electromagnetic
When using video components from KAPPA,
please comply with the supplied manual "Color compatibility (EMC)
TV camera for microscopy and 3D images".
The components for 3D microscopy have been
Special regard must be paid to the notes on designed and tested in compliance with EN
instrument safety included in this manual. 61010, Part 1 (VDE 0411). From the safety
To guarantee the safe operation and function viewpoint, they left the factory in a perfect
of the components for 3D microscopy, it is state.
necessary under any circumstances to take the They meet the requirements of the EC directive
precaution measures and observe the warnings 89/336/EC and the EMC legislation of
contained in the operating instructions. November 9th 1992 and the legal regulations
The following symbols are used for the for accident prevention.
warnings: The components for 3D microscopy meet the
EC requirements and are CE-labelled.
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3D Microscopy Notes on unpacking, transport and storage
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General
Application 3D Microscopy
2 General
2.1 Application
3D microscopy permits the stereoscopic viewing 3D microscopy is ideal for the following
of microscope specimens and objects applications in biomedicine:
up to highest magnifications micromanipulation
using all objectives patch-clamp technique
eyepiece and monitor observation in-vitro fertilization
in reflected and transmitted light examination of endocytosis and xenobiotics
on upright and inverted microscopes examination of phagocytosis
in brightfield and phase contrast injection of nucleotides
in real color examination of nerve cells
in real time
with extended depth of focus 3D microscopy is ideal for the following
with improved resolution applications in materials microscopy:
storage via recorder or image memory of the defect analysis of semiconductor
PC components
documentation via video prints or color observation of surface topography in
photos microtechniques
materials examinations in construction
engineering
The following microscopes can be upgraded
with the components for 3D microscopy: rupture analysis
Axiotron 2
examination of the structure of microfibers
Axiotech 100
examination of the structure of crystalline
materials
Axiotech 100 vario examination of compound materials.
Axiovert 100/135/135 M
Axiovert 25 CA
Axioplan 2/Axiophot 2
Axioskop
Axioskop 2
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3D Microscopy Function Eypiece version
2.2 Function
The dynamic technique on which 3D There are three different versions of 3D
microscopy is based can be used with any light microscopy:
microscope with an accessible aperture
diaphragm.
eyepiece version
The technique can be used with upright or monitor version
inverted reflected-light and transmitted-light combination of eyepiece and monitor
microscopes. version
A patent has been applied for the technique.
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Function Monitor version 3D Microscopy
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3D Microscopy Function Monitor version
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Function Combined version 3D Microscopy
2.2.3 Combination
of eyepiece and monitor version
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3D Microscopy Maintenance and care
2.3.1 General
Remove dust using a rubber blower
Use a soft optics cleaning cloth
Never expose the components for 3D
microscopy to inadmissible climatic conditions
Use a natural hair brush degreased in alcohol
and dried before.
(high humidity and temperature) for longer
periods of time. The glass plate of the window of the CCD
camera can be cleaned using the cleaning buds
The components must be protected from dust supplied.
and humidity and covered with the dust cover
after every use.
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Troubleshooting 3D Microscopy
2.4 Troubleshooting
The monitor displays colored fringing or A pseudostereoscopic image is displayed,
several out-of-focus images: i.e. heights and depths in the image are
Switch off MCU II;
exchanged:
switch it on again after 10 seconds The toggle switch of the 3D CONTROL DAS-
3 is not set correctly;
No 3D image is obtained: set toggle switch to "STEREO"
monitor shutter does not function; For observation, exchange eyepieces for 3D
check switch for monitor shutter on the shutter
control unit; the green lamp lights up only if For monitor observation,
the switches are set correctly set toggle switch of 3D CONTROL DAS-3 to
parameters on the MCU II are not set "STEREO";
correctly; if required, change "Scope LCD" parameters
check the parameters, mainly Time, in the CONFIG menu of the MCU II
MEMORY IN, MEMORY OUT For monitor observation,
camera not positioned correctly; set the switch of the control unit for the
check whether the camera controls are monitor shutter to "stereo mode"
aligned perpendicular to the stand and
whether the monitor image follows the Note:
stage motion; If inverted microscopes are used in transmitted
if not, turn the camera light - e.g. the Axiovert 100/135/135 M -
3D illumination shutter is out of alignment;
remember that observation is made from the
opposite side of the instrument if manipulators,
recenter aperture diaphragm,
needles and electrodes are used.
recenter 3D illumination shutter
Knob of 3D-CONTROL DAS-3 control units
not set correctly;
check 3D setting of 3D-CONTROL DAS-3
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3D Microscopy Technical Data
3 Technical Data
Parameters Description
Ambient conditions
Temperature + 10°C ... + 35°C 0°C ... + 35°C 20°C ... + 50°C
Safety
Protection degree I
Protection type IP 20
Pollution degree 2
Power supply
Fuses
Line input 115 VAC: T 0,2 A/250 V in accordance with IEC 127
3D-CONTROL DSA-3 230 VAC: T 0,1 A/250 V in accordance with IEC 127
For MCU II, monitor shutter, monitor and microscope, see the enclosed Technical Data sheets.
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3D Microscopy
Axioskop
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3D Microscopy
Axioskop Contents
Illustrations
Fig. 1 Overview ......................................................................................................................... 16
Fig. 2 Attachment of 3D condenser adapter .............................................................................. 18
Fig. 3 Insertion of quartz depolarizer ......................................................................................... 19
Fig. 4 Insertion of phase stop 20................................................................................................ 19
Fig. 5 Connections for eyepiece version ..................................................................................... 20
Fig. 6 Changing the voltage selector on the rear of 3D-CONTROL DSA-3 .................................. 20
Fig. 7 3D-CONTROL DSA-3, front .............................................................................................. 21
Fig. 8 Insertion of 3D illumination shutter in the 3D condenser adapter ................................... 22
Fig. 9 Adjustment of 3D illumination shutter, pupil ................................................................... 22
Fig. 10 Labels to mark the eyepieces and plugs............................................................................ 24
Fig. 11 Wiring diagram for monitor version ................................................................................. 25
Fig. 12 Attachment of monitor shutter ........................................................................................ 26
Fig. 13 Rear of MCU II ................................................................................................................. 26
Fig. 14 Attachment of camera ..................................................................................................... 27
Fig. 15 MCU II controls, front ...................................................................................................... 28
Fig. 16 Wiring diagram for video prints....................................................................................... 30
Fig. 17 Wiring diagram for eyepiece and monitor version ............................................................ 31
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3D Microscopy
Overview Axioskop
Fig. 1 Overview
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3D Microscopy
Axioskop Overview
Axioskop
BNC cable
Key to Fig. 1
1 3D-CONTROL DSA-3
2 Quartz depolarizer
3 Eyepieces with 3D shutter and phase stop 20
4 3D illumination shutter
5 Condenser adapter
6 Condenser carrier
7 TV adapter
8 Camera
9 MCU II control unit
10 Control unit for monitor shutter
11 17“ monitor
12 Monitor shutter
13 Polarizing glasses
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3D Microscopy
Eyepiece version Axioskop
2 Eyepiece version
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3D Microscopy
Axioskop Eyepiece version
Insert depolarizer:
Fig. 3
Remove tube
Insert depolarizer in the open aperture in
the stand and lock it in position
Attach tube again
1 Quartz depolarizer
2 Locking screws
Fig. 3 Insertion of quartz depolarizer
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3D Microscopy
Eyepiece version Axioskop
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3D Microscopy
Axioskop Eyepiece version
STEREO/PSEUDO
Toggle switch which determines the phase
position between the shutters.
In the "STEREO" position, a stereoscopic
(upright) image is created, while the image in
the "PSEUDO" position is pseudostereoscopic
(vertically reversed).
Fig. 7 3D-CONTROL DSA-3, front
Rotary switch for the setting of different
modes:
STEP step mode
(inclined illumination from the
left or right)
2D brightness position if toggle
switch is in "STEREO“ position
3D VIS 3D image, eyepiece version
3D VIDEO 3D image, monitor version
E1, E2
Potentiometer for voltage adjustment of the
eyepiece shutter (factory-aligned);
E1 acts on the left EYEPIECE socket;
E2 acts on the right EYEPIECE socket
2.2.1 Connections I1, I2
Fig. 7 Potentiometer for voltage adjustment of the 3D
EYEPIECE illumination shutter (factory-aligned)
Connection sockets for eyepieces with 3D I1 acts on one side of the
shutter 3D illumination shutter,
I2 acts on the other side of the
ILLUMINATION SHUTTER 3D illumination shutter
Connection socket for 3D illumination shutter
TRIGGER
Trigger input socket for connection
of the MCU II
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3D Microscopy
Eyepiece version Axioskop
2.3 Adjustment
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3D Microscopy
Axioskop Eyepiece version
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3D Microscopy
Eyepiece version Axioskop
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3D Microscopy
Axioskop Monitor version
3 Monitor version
The following components from KAPPA are Control unit for monitor shutter with power
required for the monitor version: unit and connecting cable
MCU II control unit 3D illumination shutter
CF15/4 MC-S camera Control and supply cable between
CF15/4 MC connecting cable for MCU II
MCU II and 3D illumination shutter or
monitor shutter
Line cable
Polarizing glasses
Monitor (100 Hz) with connecting cable and
attached monitor shutter
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3D Microscopy
Monitor version Axioskop
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3D Microscopy
Axioskop Monitor version
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3D Microscopy
Monitor version Axioskop
LCD-Display
Display of menu options
Control keys
Arrow keys for options and settings within the
menus
Mode
Key for menu switching
Freeze
Key for storage of the current image;
the stored image is constantly made available at
the video ports
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3D Microscopy
Axioskop Monitor version
3.3 Adjustment
Use microscope slide as an object and focus
on it using the 10x objective;
after adjustment, check setting using the
objective of the highest magnification
Check center position of the 3D illumination
shutter in the same way as for the eyepiece
version
(page 22/Fig. 9);
for this, remove the camera cable from the
MCU II or from the camera:
(page 26/Fig. 13)
Place the required object on the stage and
focus on it
Switch beam path to camera/TV
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3D Microscopy
Monitor version Axioskop
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3D Microscopy
Axioskop Eyepiece and monitor version
4 Eyepiece and
monitor version
4.1 Conversion and adjustment
Set up and configure the microscope in
accordance with the operating instructions
of the Axioskop
Set up the eyepiece version
see page 18
Before connecting the components, first read Set up the monitor version
the enclosed manual from KAPPA entitled see page 25
"Color TV camera for Microscopy and 3D
Images"; Connect eyepiece and monitor version in
chapter 5, "Safety regulations", should be accordance with the wiring diagram
observed in particular. Fig. 17
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3D Microscopy
Eyepiece and monitor version Axioskop
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