An experiment is carried out to investigate the effect of total surface area on the rate of diffusion of substances in an organ. Potato blocks with different number of triangular surfaces are used in the experiment. Preparation of the experiment is shown in the following diagrams. Satu eksperimen telah dijalankan untuk menyiasat kesan jumlah luas permukaan terhadap kadar resapan bahan dalam organ. Bongkah kentang dengan bilangan permukaan segitiga yang berbeza digunakan dalam eksperimen. Persediaan eksperimen ditunjukkan dalam rajah di bawah. Step 1 Langkah 1 Sem ——— A potato block is cut so that it will have four triangular surfaces, with the ‘measurements shown Bongkah kentang dipotong untuk menghasilkan — empat_—_permukaan segitiga dengan ukuram di atas. Step2 Langkah 2 6 co rom top om dari ‘A cylindrical hole is made in the middle of potato block, 6 cm long , by cutting the potato block from top using a cork borer. Satu lubang silinder dibuat ditengah- tengah bongkah — kentang, dengan memotong Gem dari atas bongkah tersebut menggunakan penebuk gabus.Step 3 Langkah 3 A 10% salt solution is poured into the hole in such a way that the height of the solution in the hole is 5 em. 10% larutan garam dituangkan dalam lubang tadi setinggi Sem. ke Step 4 Langkah 4 Capillary tube Tiub kapitari Rubber stopper Penutup getah A rubber stopper with a capillary tube inserted through it is prepared as shown in the figure above. The rubber stopper is used to close the hole containing salt solution. Tiud kapilari dimasukkan ke dalam penutup getah seperti yang ditmjukkan dalam gambar persediaan di atas. Pemutup getah digunakan untuk menutup lubang yang mengandungi larutan garam.Initial level of 10% salt solution (A) Aras awal 1026 larutan garam (A) + The hole in the potato containing salt solution is covered with the stopper prepared in step 4. + Salt solution rises up the capillary tube. Initial level of salt solution is marked as A. * Another mark is made on the capillary tube ata distance 3 em above the point A. It is labelled as B, ‘* Lubang dalam kentang yang ‘mengandungi larutan garam itu ditutup dengan menggunakan penutup dijalankan dalam langkah 4 + Larutan garam menaik dalam tiub kapilari, Aras awal larutan garam ditandakan 4 © Tandaan yang berikutnya dibuat pada tiub kapilari pada jarak 3cm Ja dilabelkan dengan B. Step 6 Langkah 6 Distilled water Air suling Place the potato block complete with the rubber stopper and capillary tube ina beaker containing distilled water Make sure the potato block is not completely immersed in water Water molecules diffuse across the potato tissue from distilled water to salt solution Record the time taken for the salt solution to travel from A to B. Letakkan bongkah kentang yang dilengkapkan dengan pemutup getah dan tiub kapilari ke dalam bikar yang mengandungi air suling. Pastikan bongkah kentang tidak tenggelam sepenubnya di dalam air. Molekul air akan meresap merentasi lisu kentang dart air suling ke larutan garam. Rekod masa untuk larutan garam bergerak dari A ke BSteps | to 6 is repeated u: ig potato blocks of the same size, but different number of triangular surfaces, that is potato block with 8 and 16 triangular surface. Langkah 1 hingga 6 diulang menggunakan menggunakan saiz bongkah kentang yang sama, tetapi berbeza bilangan permukaan segitiga, iaitu 8 dan 16 permukaan segitiga. Table 1.1 shows the result of this experiment. Jadual 1.1 menunjukkan keputusan eksperimen ini. Number of Distance | Time taken for salt solution to | 5 triangular surfaces | A to B move from A to B/min. —* ee Bilangan Jarak Masa yang diambil wntux e "S| permukaan segitiga | Ake B | larutan garam bergerak dari A | ke B/min <—bsB Time / masa: ‘Time / masa:16 3cm Time / masa: .. Table 1.1 Jadual 1.1(a) Record the time taken for the salt solution to move from A to B in the spaces provided in Table 1.1 Rekod masa yang diambil oleh larutan garam untuk bergerak dari A ke B dalam ruangan ‘yang disediakan dalam Jadual 1.1. (3 marks ] (b) @ State two different observations Based on Table 1,1. Nyatakan dua pemerhatian yang berbeza Berdasarkan Jadual 1. Observation 1: Pemerhatian |: Observation Pemerhatian 2 [3 marks } (ii) State the inferences from the observations in 1(b)(i). Nyatakan inferens daripada pemerhatian di 1(b)(i) Inference from observation 1 : Inferens daripada pemerhatian 1 : Inference from observation 2 : Inferens daripada pemerhatian 2 : [3 marks](©) Complete Table 1.2 based on this experiment. Lengkapkan Jadual 1.2 berdasarkan eksperimen ini, ‘Variable Method to handle the variable Pembolehubah Cara mengendali pembolehubah Manipulated variable Pembolehbah dimanipulast Responding variable Pembolehubah bergerak balas ‘Constant variable Pembolehubah dimalarkan Table 12 Jadual 1.2 [3 marks ] (A) _ State the hypothesis for this experiment. Nyatakan hipotesis bagi eksperimen ini. (G3 marks ](©) @ Construct a table and record all the data collected from Table 1.1. Your table should have the following titles: Bina satu jadual dan rekodkan semua data yang dikumpul dari Jadual 1.1 Jadual anda hendaklah mengandungi tajuk-tajuk berikut: + Number of triangular surfaces Bilangan permukaan segitiga © Time taken for the salt solution to move fiom A to B. Masa yang diambil untuk larutan garam bergerak dari A ke B © Rate of diffusion. Kadar resapan Rate of diffusio Distance of salt solution move from A to B ime taken for the salt solution to move from A to B. Kadar resapan = Jarak larwan garam bergerak dari A ke B ‘Masa yang diambil untuk larutan garam bergerak dari A ke B [3 marks} (©) Gi) Use the graph paper provided, draw graph the rate of diffusion against the number of triangular surface. Dengan menggunakan kertas graf yang disediakan, fukis graf kadar resapan melawan bilangan permukaan segitiga [3 marks]Graph of the rate of diffusion against the number of triangular surface, Graf kadar resapan melawan bilangan permukaan segitiga(Based on graph in 1(¢)(i), explain the relationship between the number of triangular surface and the rate of diffusion. Berdasarkan graf dalam di 1(e)(i), terangkan hubungan antara bilangan permukaan segitiga dengan kadar resapan. [3 marks ] (g) Based on the result of this experiment, state the operational definition for rate of diffusion Berdasarkan keputusan eksperiment, nyatakan definisi secara operasi bagi kadar resapan, [3 marks] i) ‘The experiment was repeated by using 30% salt solution instead of 10% sat solution, Predict the outcome of this experiment ifthe student used numberof triangular surface of potato block is 16. Explain your prediction, Eksperimen diulang dengan menggunakan 30% larutan garam bagi menggantikan 10% larutan garam. Ramatkan hasil eksperimen ini jika pelajar menggunakan bongkah kentang yang mempuonyal 16 permukaaan segttiga, Terangkan ramalan anda (3 marks}@ Which of the following organs can be classified as the organs that increase the total surfaces for their effective functions, Yang manakah organ-organ berikut boleh dikelaskan kepada organ yang meningkatkan jumlah permukaan untuk fungsi yang lebih efektif. ‘Small intestines Usus kecil Tungs parwparu heart Jjantung esophagus brain pancrease esofagus otak pancreas liver hati Organs having well adapted surfaces for | Organs without surfaces well adapted for their effective function their effective function Organ-organ yang penyesuatan Organ-organ tidak mempunyai permukaan untuk fungsi yang efektif | penyesuaian permukaan untuk fungsi yang efelif Table 1.3 Jadual 1.3 (3 marks]Question 1 No Mark Scheme Score ‘KBOG03 — Measuring Using Number Able to record all 3 readings for the time taken far salt solution to ‘move from mark A to mark B. Number of triangles surfaces Time taken/min 3 4 2 8 2B 1) i 16 13 —————__| Able to record any 2 readings correctly 2 Able to record amy 1 reading correctly. 1 No response of one reading ° KBO601_- Observation ‘Able to state two correct observations based on the following aspect : P1: Number of triangles surfaces P2:: Time taken for salt solution to move from mark A to mark: B Sample answer 1, When the number of triangles surfaces is 4, the time taken for salt solution to move from mark A to mark B is 42 3 minutes. 2. When the number of triangles surfaces is 16, the time taken 1 for salt solution to move from mark A to mark B is 13 oxo soinues 3. Increase the number of triangles surfaces increase the time taken for salt solution to move from mark A to mark B. Able to state one correct observation and one inaccurate observation or able to state twa inaccurate observation. Sample Answer: 1. When the number of triangles surfaces is 4, the time taloen for salt solution to move from mark A to mark B is the long.2, When the number of triangles surfaces is 16, the time taken for salt solution to move from mark A to mark B is shortest, 3. The number of triangles surfaces affects / influence the time taken for salt solution to move from mark A to mark B. Abie to state one correct observation or able fo state two ‘observations at idea level, Sample Answer: 1, Time taken for Time taken for salt solution to move from mark A to mark B are 42 min / 28 min/ 13 min. 2, ‘Time taken for Time taken for salt solution to move from mark A to mark B increased / decreased 3. Time taken for Time taken for salt solution to move from mark A to mark B change. No response or wrong response. Scoring Correct | Inaccurate] Idea] Wrong] Score 2 : - 3 1 1 - - 22. Multcellular organisms are bigger and complex. The cells cannot depend on diffusion alone to obtain its requirements. Multicellular organisms need the transport system to transport respiration gaseous and nutrient to the inner part of the body. Whereas, unicellular organisms get thelr gaseous and nutrients supply by diffusion though their cell membrane because thelr total surface area per volume are big. Based on the above information, plan a laboratory experiment to study the relationship between the size of organisms and the rate of diffusion ‘The planning of your experiment must include the following aspects Organisma multise! adalah besar dan kompleks. Sele! tidak boleh bergantung kepada proses ‘esopan sahaja untuk mendopatkan keperluannya. Oganisma multise! memerlukan sistem pengangkutan untuk mengangkut gas-gas respirasi dan bahon nutrien ke bohagian dalam seluruh tubuhnya. Manakala, ‘organisma unisel mendapatkan bekolan gas repirasi dan bekalan nutriennya secora resapan menerusi ‘membran sel kerana jumloh luas permkaan per isipadunyo yang besar. Berdasorkan makiumat diatas, reka bentuk satu eksperimen makmal untuk menentukan hubungan ‘ontara saiz organism dan kadar resopan. erancangan eksprimen anda hendakloh meliputl aspek-aspek berikut: + Problem statement Pernyataan masoloh = Hypothesis Hipotesis ~ Variables Pembolehubah + Uist of apparatus and materials Senarai radas dan bohan + Experimental procedure Prosedur eksperimen Presentation of data Persembohan data [27 marks/markah]‘Question 2: = = aa Ann [frp eet at eau apeenneomrcn |. me Siete re, |tmontemamnietatennestdnomcan few ime Vevcnw oeetcden tna ion es ut ent Tiypothesis | Wore calular components can be sean in plant call compared Io mua eat Pte eto en snare ines a — RETR Spee Svea [emo SON aso ee oy ert sna mye re [sem aa a RGR RN a Seo ees ras [pron sp otne eos qd Epis Sa ata Ras emarman aera rae = |Scpaeneer in Gene a tet ime combed merce ‘= Procedure ‘TObiain scale feat hom onion bulb ee mewn owe racer [Rut hee mcaet sr a0 TRlsscrate te Tat rte jiininivas fete eran Shai babel naka wd, cowrthe einen with ever "| FA a «im | Pointe dian sannnwerindtoe a ere Seam Susie sin tee "esas 1 {cpa te ooh nam Lape ter grams tae oman Shake tastme Se eae Dienwomneweae io ona, =‘Observation Correct imark Labeling — membrane cell,cell wall nucleus , vacuole {mark “Magnification 1 mark Conclusion | There are more celular components that can be seen in plani cel ‘under light microscope compared to animal cel {mark Hypothesis accepied, Planning ‘Able to stato comectly KB061203 T aspects (7 ticks) - 3 marks 5/6 aspects (5/8 ticks) - 2 marks 3-4 aspects (3-4 ticks) - 1 mark <3 = Omarkwe. ‘Able to state Wo inferences correctly based on the felbowing, aspect: PI : surface area. 2 difflasion of distilled water / rate of diffusion ‘Sample answer 1, @ triangular) increase / more the total surface area (of the potas bieck), increase rate of diffusion more {water will sdiffuse into the potato block. 2, (16 triangular) decreate total surface area of the potato block ‘is the docterase, rate of diffusion of (water) int the putata bblock: 3. The surface area of the potata blscic is the loveet/highest, rate of distilied water diffusion i bowest/aighest hey ‘Able to make ene corsect inferences and one inateurale inference ‘Sample answers, 1. The rate of diffturion is highest / lowest. 3. The total sorfices area is the highest / lowest. ‘Able ‘o sinit eee correct inference and one inference at idea level,‘KHO610-Controfling variables ey ‘Vacishiles “Able to stain all'S variables and the 3 methods to Bandlle the variable comecttly. ‘Sample Answer “Method ta Handle the varkable | correctly ‘Number af triangles surfaces / Total surface salt salution to move from mark A te mack BY Rate ‘ef Diffusion, [ao OO ‘Responding variable | Measure and resond the tine taken ‘Duration! Time taken for | for salt solution to move fro mark ‘Use different number of triangles (Surfaces which are 4, 4, 16 \v ‘Change the nuienber of triangles Surfaces that ane 4, B, 16, Ato mark B by using stopwatch, Calculate the rate: of diffusion by ‘ing the Sornscla: Distance from A to B een ‘Time taken for sail substion te move from mark A to mack B, min Constant variably ‘Coneentration of salt j)falitoe Distance fram || Ato B/ Size of potato | block Fix the ume concentrating of salt solution that is 10%. / Fox the mame distance from A to B whieh bt 3 cm / used the same size of potmin Iilock is Sem TemTia ‘KB0611-Stute bypothesis ‘Able to make.s hypothesis based oa tive follewing aspect : Pl: Manipalated variable | Number of triangular surface / total surfane P2: Respanding variate ; Tima taken for eadt solution move from ‘Ato B / rate of diffesion | R : Relaponehip | Sample Answer: 1, The bigher / the number of triangles surfaces, the shorter the time taken for salt coluting to move from A bo B/ vie verda 2. The bigher the total surfaces area of putata lack, the shorter the tate taken for salt solution te move fom A to BL vive ver 43, The higher the rumber of trinngles surfaces, the higher the rate of water diffusion / vice versa 4. The higher the total surfaces area of potato luck, the shorter ‘the time taken for salt solution 10 move from A to BY vice vere ‘Able te makes hypothesis relading the manipulated variable and responding variable inmecursicly Sample Answer: 1. Diffcreat numbcwlsize of triangles sarfnocs, diffcrent the time taken for selt polation to move from A to B. 2, The oumbesaize of riangles warfaces, affects the time taken for malt solution to mae from A tn B. 3. The mumber/size of triangles surfaces, effects the rte af water ddiffasion ‘Able in state a hypatheats relating the manipulated vartable at dea level, with ane aypect correctly, ‘Sample Answer 1. Different nunsber / size of triangles 2 Different rate of diffusion | No response or wrong respouse if no Pl or P2 no mark far each,KB0606 data ‘Able to construct a table ‘contain the Fallowing axpects PL: Able to state the 3 titles with units correctly, PD: Able to record all deta correctly (FS: Able w calculate the tke of diffusion ye ars wer Meli) (No response or wrong response, o CL KBO607 ~ Relatiwnship between space and time ‘Able to plet the graph correctly Criberia P: Comect Axcis with label, uniform scale and unit °¥ axis, Rate of Diffusion Xaxis, Number of tiangles surfaces T: All points plotted engi) Pein alt 3 points | Any two criterts 2 Any ome criteria. 1 [No response or wromg respomse. Lp,the kcaeps eduts. ‘Able to explain the relationship between the number of trlamguler surface and the rate of differen accurately, Samnple anewer: ‘RU; Increase the sunmsber of trinngulst surface increase the rate of diffasion of waster Bl Increase total surface area ‘52: Mone water molecule dalle in the potata block in the shart time ‘All three aspects Note: [Ki wrong, reject Eid E2 Two aspects inchading Ri Example: R1+E1/Rl+ £2 ‘Only BE stated 1 2 _No response or incorrect response a ‘KBO609 — Defining by operation Able to state the opezational definilsan of rate of diffasion based on the following aspects; Pi: Shows time taken ef sall eatuting move fhoa A to B 72 sin different number of triangular surtsce of potato block 72: increase the total murfice of potato, increase rate af diffusion ‘of water /! rate of diffusion of warer depends om the total narface af potate block Sample answer ‘The rate of diffusion i time taken for salt solution to move from A to B when different nunsben'size of triangles warfare is wsed. ‘nerease the total sarface area of potato block, inerenee the rute of j Risin oF (lined wate) ttre ini the por block. Any tw criteria stated Any one criterin stated /Y theoretical definition ‘No response or wrong response,Predicting KBOOS ‘Able-te predict uring all the éllowing criteria - Pl = Correct prediction , ete the value El: Reason E2- Effect Sample answer PL: Tite talaen foe salt solution meve fiom A to B is fess than 13 minutes J? any vali less then | 3rminutes EL: High gradient concentration between distilled waicr and 30%, ‘alt solution ED: The rate of water diffusion. imp potato block wall be faster / Increase! moce than O.23emmisartes” 7 More watsy molecsle ‘diffuse inte potato block im the short tims. Any twe aspects Pi idea) + 2B. P1 +16 PL+B Any one aspect : P{ idea) + E11 PH (idea) + 2 No response or wrong Fespanse ‘KBO60S — interpreting the data Mi) Able ta clarify all the organ in table correctly: Abile to pet 4-6 tick correctly‘Able a stake a prablam statement relating the manipulated variable wath Oe responding variable correcty. Fi: MV-~total surface area pervalume Fat RV —ate ef eFluston Pa: Cuestion: Sample answer ‘what ice relator between total surface area per velume ratio and the rate ef Psion? ‘Abie to stuie « problem statemest inaccurately Sample ancaer: what U the relstionship between tatal surface area per valume on dithusion? “Abie to rtmte «problem cutamant at idea level Sample answer. ‘Total surfaet area per volume Influenced the diffusion ‘Able to state a problem statement st idea level a “Abia Yo state Rypothesis lating the manipulated variable tu the responding variable cenrrectly, Fi: MW = total surfoce aren per volume PE: RV =the rate of diffusion FE Relationship ‘Sample anawer ‘Tht larger the total surface area per voluene ratio / the smaller the sine cf cube, the ‘jacter the rate of éiffusion, ‘Able to state a hypothesis accurately ‘Sample anaveer: “Total surface area ptr volume ratla influenced the rate of diffusion ‘Abie to state w hypothesis at ides level Sample answer: ‘There isa relationship between the total surtace area per youre ratio are the rate of cattusion, (No respanse or incareet response‘Able to state wl three variables correctly ‘Sample aeauer: ‘Manipulated varlable: Tetal suefoce area per volume ratio the alte of cubes. Responding variable: Rate of elffusion, Fined varlatt: Colored water temperature / time tater, ‘Able to-state any twa variables comecthy ‘Able to-state any one variable correctly (No response or Incorrect response ‘Able to ist all the important apparstus and materials correct Semple answer: acetals: 21 Potato 2, Colored water, ‘Apparatus: L. Penknite 2 White tiles 2 Beaker 4 Forceps 5 Basia 6. Gridded Abin to bet atleast 4 appacatur and 2 materials correctly ‘Abie 10 Het et least Zapparatat and Zmatedals comectly ‘Na response or Inorrect respondeSample answer: Procedure: water, ‘Albthe: cubes are left far BO: minutes. ‘aed cut into two halves, transparency. Soe ee Ne Formula: Rate of dfuston = Percentage of emloced arse [%éruin%) Time taken 7. The resumy ave recorded In the table, 8, Beware of using ite Note: XE: Sbepe 1, 2,3, 4, 5 (preparation of materials ed angaracusl (2: Steps 2, 3 (operating fixed variable} 1S: Stepe 6 [operating responding variable} 18 :Stepe 2 [operating manipulated voribla} AS: Steps & (precawtion) Biv) | Atieta describe the steps of the experiment procedure or method omreciiy Potato are cut nto O.$.om, 2.0m, 3 cm and 4 em langrn of wes cubes each, Then the cubes.are immersed into wliatin containing 100ml ef colored ‘Afar 20 rainates, the outer surface ofthe cubes are dried wath fitter paper ‘The percentage of colored ares in each cube i estimated by ising s girded ‘The rate of diffusion of each cube is calculated by using & formmula: r athe? ‘Any 46 lz Any dK 1 ‘Only 4 Korna response arincoiredt response 0 “Able to predent allthe data with units correctly z Sample answer: Cube | Lenethot | Total Volume | TS4/v | volume of | Percentage |) sda fem} surface | om? | rata | stained | of coloured area om” ont part ore a | os 1s oars | a ® 1 6 2 ‘ c i 4 a a o a u 2 E 4 96 a us AASie to present a table wth ot least 5 ies [ Mo response oF intarrect rasponae‘An experiment was carried out to study the effect of enzyme concentration on the activity of ‘amylase on starch. Satu eksperimen telah dijalankan untuk mengkaii kesan kepekatan enzim ke atas akiivti amilase ke atas kanji. ‘The following steps were carried out. Langkah-langkeh berkut telah dijalankan. Step 1 ‘Three test tubes P, O and R were filed with 2m| of 0.5%, 1.0% and 1.5% of amylase respectively Langkah 1 Tiga tabung yi P, Q dan R dilsi dengan 2m! larutan 0.5%, 1.0% dan 1.5% amilese masing-masing. Step 2ml of 1% starch suspension was added into each test tube and the contents were stirred. Langkah 2: 2m! ampaian kanji 1% dimasukkan ke dalam setiap tabung wi dan carmpuran dikacau. Step 3: Every 2 minutes a drop of the mixture from each test tube was mixed with a drop of iodine solution on a white tie. Langkah 3: _Setiap 2 minit setitik campuran daripada setiap tabung ui dicampurkan ke dalam otitis larutan iodlin datas jubin putin Step 4: Alltest tubes are immersed in a water bath at temperature of 37°C during the experiment as shown in diagram below. Langkeh 4: —_Kesemua tabung didi clrendam dalam kukus air pad suhu 37°C sepanjang ‘masa eksperimen seperti yang ditunjukkan dalam rajah di bawah Diagram ‘shows the apparatus set-up used in this experiment. Rajah 1 menunjukkan bahan-bahan yang digunakan dalam eksperimen tersebut. | __ Water bath 37°C Kukus air 370 |__ Amylase + starch ‘Anilase + kanjiTable 1 shows the results of the experiment. Jadual 1 menunjukkan keputusan eksperimen. Test tube The colour of iodine solution staring From minute 0 Wara larutan iodin bermula dari minit 0 Tabung ui Dark blue Biu tua change) Kuning (tiada porubshan) Yellow (no @ ° ‘Time taken for the starch to be hydrolysed completely(min) Masa diambil bagi kanji dihidrolisiskan dengan lengkap (min) @©@®©OOOO ®®@O000 OOOO0O0O |©©©O@® ©00000 ©00000 @®©@@©OOO OOO0OO ©O09000O Table 4 Jadual 1fa) (o) ii) Record the time taken for the starch to be hydrolysed completely in the boxes provided in table above, Rekod masa yang diambil bagi kanji dihidrolisiskan dengan lengkap di dalam kotak dalam jadual di atas. [3 marks} [3 markah) Based on Table 1, state two different observations. Berdaserkan Jedual 1, nyatakan dua pemerhatian yang borbeza. Observation 1 Pemerhatian 1: Observation 2: Pemerhatian 2: [3 marks} [3 markah] State the inference for each observation made in 1(b) (i) ‘Nyatakan inferens daripada pemerhatian 1 (b) (). Inference for observation 1 : Inferens daripada pemerhatian 1: Inference for observation 2: Inferens daripada pemerhatian 2: [3 marks) [3 markah)(o) Complete Table 2 to show the variables involved in this experiment and how these variables are operated Lengkepkan Jadual 2 untuk menunjukken pembolchuban- pembolehubah yang terlibat dalam eksperimen ini dan bagaimana pembolehubah-pembolehubah inl dikendalikan. Variable ‘Operating the variable Pembolehubah Cara mengendalikan pembolehubah Manipulated variable How to alter the manipulated variable: Pembolehubah yang Bagaimana pembolehubah yang dimanipulasikan dimanipulasikan diubah : Responding variabie: Howto determine the responding variable Pembolehubah yang Bagaimana pembolehubah yang bergerak bergerak balas: balas ditentukan : Constant variable: How to maintain the constant variable: Pembolehubah yang Bagaimana pembolehubah dimalarkan dimalarkan: ditetapkan: Table 2 Jadual 2 () State the hypothesis for this experiment. Nyatakan hipotesis bagi eksperimen ini [3 marks] [3 markeh] “TS marks ] [3 markan }fe) (i) Construct a table and record all the data collected in this experiment. Your table should have the following aspects : Bina satu jadual dan rekodkan semua data yang aikumput dalam eksperimen ini. Jadual anda hendaklah mengandungl aspek-aspek berikut + Enzyme concentration Kepekatan enzim + The time taken for starch to be hydrolysed completely, 4 (minute) ‘Masa yang diambil bagi kenji dihidrolisiskan dengan lengkap, t (minit) + The rate of enzyme amylase activity, 1/t (minute) Kadar aktiviti enzim amilase, 1/t (minit?) [3 marks} [3 markahy Based on the data in 4 (2) (i), draw a graph to show the relationship between the enzyme concentration and the rate of amylase activity Explain your answer. (Use the graph paper provided on page 7 to answer question.) Berdasarkan data ci 1 (e) (), lukis satu graf untuk menunjukkan perhubungan antara kepekatan enzim dengan kadar aktiviti enzim amilase. Terangkan jawapan anda. (Gunakan kertas graf di halaman 7 untuk menjawab soalan ini)Graph of the rate of enzyme amylase activity against enzyme concentration, Graf kadar aktiviti enzim amilase melawan kepekatan enzimo (a) om) Based on the graph in (e) (i), state the relationship between the enzyme ‘concentration and the rate of amylase activity. Explain your answer. Berdasarkan grat di (e) (i), nyatakan perhubungen antara kepekatan enzim dengan kader aktivti enzim amilase. Terangkan jawapan anda, (3 marks] [9 markan Based on the experiment, define operationally what enzyme is. Berdasarkan eksperimen, benkan definis| secera operasi bagi enzim. [3 marks) (3 markahy The experiment is repeated with test tube R immersed in water bath with temperature of 65°C during the experiment. Predict the time taken for starch to be hydrolysed completely in test tube R. Explain your answer. Eksperimen diulang dengan merendam tabung ui R dalam bekas kukus air bersuhu 65°C semasa eksperimen. Ramatkan masa yang diambil bagi Kenji dihidrolisiskan dengan lengkap dalam tabung uji R. Terangkan jawrapan ande, [3 marks) {3 markeh}@ ‘The following are some of the materials used in the experiment to study the effect of pH on the enzyme activity. Yang berikut adalah beberapa bahan yang digunaken dalam eksperimen untuk mengkaji kesan pH ke aias aktiviti enzim. - Sodium hydroxide solution Laruten natrium hidroksida ~ Hydrochloric acid Asid hidrokforik - Distilled water Air suling Complete the following table to classify the materials according to the type of medium. Lengkapkan jadual berikut untuk mengelaskan bahan-bahan tersebut berdasarkan jenis medium. Material Type of medium al | Bahan Jenis medium | [8marks] [3 markah]