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Nanoparticles have become an integral part of research. They can be applied in almost every field,
from medicine to substitutes of heavy metals. These particles can be synthesized by chemical
reduction of silver ions in aqueous solutions, thermal decomposition in organic solvents, with the help
of stabilizing agents, chemical reduction, photo reduction and `biosynthesis of the Nano particles. As
compared to the Biosynthesis of Nano particles, other methods are expensive and pose a threat to
ecology due to the usage of harsh and hazardous chemicals. The biological synthesis of Nano particles
involves the use of bacteria, fungi, algae and leaf extracts. The major advantage of using this kind of
synthesis is that it has zero chemical toxicity on the environment which is why they are considered to
be eco-friendly in nature and hence they are used in various biomedical applications. Research in the
past has proved that Nano particles have antimicrobial activity. Green synthesis of Nano particles is a
breakthrough research which is being used in modern day science. Even though fungi, bacteria and
algae produce Nano particles, the most opted variant is the tea leaf since those microorganisms can
cause allergic reactions which is not observed in the case of plant extract. Bio reduction of silver ions
to yield metal nanoparticles using living plants is what researchers focus on now. Many plant variants
produce bio nano particles but we would be focusing on the Neem plant- Azadirachta indica which
belongs to the Meliaceae family. Found in tropical and subtropical regions, the plant is proven to have
medicinal activity and almost every part of the tree is used. The principle objective of this review work
is to analyse the effect of concentration of Neem leaves, temperature of synthesis, contact time
between reactants and effect of pH on the synthesis of AgNPs from the research performed

Literature review