6/5/2018 Antioxidant properties of different fractions of Vitex negundo Linn.

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Food Chemistry
Volume 100, Issue 3, 2007, Pages 1170-1176

Antioxidant properties of different fractions of Vitex negundo Linn.

Om Prakash Tiwari, Yamini B. Tripathi

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https://doi.org/10.1016/j.foodchem.2005.10.069 Get rights and content

Abstract
Vitex negundo Linn. (VN), belonging to family Verbenaceae, is an aromatic shrub distributed
throughout India. In the ayurvedic system of medicine it is used as a drug of choice to manage
pain, inflammation and other related diseases. It contains many polyphenolic compounds,
terpenoids, glycosidic iridoids and alkaloids. Since polyphenolic compounds have high
antioxidant potential, the antioxidant potency of V. negundo was investigated by employing
various established in vitro systems, such as 2,2′-azino-bis 3-ethyl benzothiazoline-6-sulfuric
acid (ABTS∗+)/Lipid Peroxides (LPO)/Superoxide/Hydroxyl radical scavenging and iron ion
chelation. Total antioxidant capacity was determined by the assay based on the preformed
radical monocation ABTS∗+. Lipid peroxidation was assessed in terms of thiobarbituric acid
reactive substances by using egg yolk homogenates as lipid rich media. Superoxide radical
scavenging assay was based on the riboflavin-light-Nitro blue tetrazolium (NBT) system.
Hydroxyl radical trapping potential was determined by evaluating hydroxyl radical induced
deoxyribose degradation using the thiobarbituric acid method. In order to assess the metal
chelation properties, hydroxyl radical induced deoxyribose degradation was evaluated in the
absence of Ethylenediamine tetra acetic acid (EDTA). All the polar fractions significantly
showed trapping of free radicals, and thereby inhibition of lipid peroxidation, and also chelated
the iron ion. Interestingly, the hexane fraction did not show any activity against superoxides
radicals and it had minimum trapping potential for other free radical (FR) species also. Thus, it
may be concluded that the polar fractions of VN possess potent antioxidant properties, which
may be mediated through direct trapping of the free radicals and also through metal chelation.

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6/5/2018 Antioxidant properties of different fractions of Vitex negundo Linn. - ScienceDirect

Therefore its reported anti-inflammatory properties, could be through the down regulation of the
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inflammation.

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Keywords
Vitex negundo; Antioxidants; Metal chelation; Inflammation

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