DETOXIFYING-

ANTI-POLLUTION

TRAINING
EXOSSINE™: EXO-P™
Exossine Technical Agenda

• Introduction on protection, claims & consumers

• Exo-P: Protection
– In vitro assay (heavy metal chelation & UV protection)

– Ex vivo assay (pollution protection)

– Anti-pollution consumer test

• Toxicological studies

• Applications

• Conclusion
What are we protecting our skin from?
We protect ourselves from things that make us look bad & unhealthy

Before Now
• UV • Elements (extreme weather, pollution,
• Aging process heavy metals)
• Skin sensitivity & intolerance • Our own physiological changes
(hormones)
• Man-made factor
(electromagnetic waves, cigarette)
Effect of pollution on skin
Rashes, allergies & skin sensibility

Takes out the oxygen from the skin cells = dull & saggy skin

Complexion becomes grayish

Interferes with the skin ability to regulate moisture = dehydration

Increases the oxidation of sebum, pimple formation = acne

Skin cells do not have proper defenses against man-made pollution

Increases free radicals and the effect of UV radiation

Decrease collagen & elastin production = rough skin & fine lines

Premature aging!
Global Protection Market
Market Info
• Since January 2008: 4624 products were launched with the «Protect against harsh
elements, pollution, smoke, environmental aggressors, urban damage claim»

• Important evolution since 2008.

Product segmentation
Evolution since 2008

+40%

40%
 Global Protection Market

Consumer Info

• Who buys «Protective products» and why?

- Consumers who have sensitive skin and reactive skin.

Why? To reduces or minimize unwanted side effect of sensitive & reactive skin
(redness, irritation, etc).

- Consumers who live in big polluted cities and who desire a maximum skin
protection from pollution, sun and other elements.
Why? To minimize as much as possible skin damage and reduce dull skin that
come with skin submitted to pollution.

- Consumers who have a preventive approach to skin aging and photo-

protection (suncare).
Why? By reinforcing skin defenses it will help fight the skin aging process and
maintain as long as possible a youthfull appearance.
Global Protection Application
Many Different Applications
• Moisturizing care & radiant skin care
• Protective care
• Sensitive skin
• Daily care
• Sun care
• Hair care What we are offering: Exo-P

DoshaCare SkinMedica
Exotic expolysaccharide and sustainable activeTNS
Environmental Shield
that provides
Ultimate Daily Moisturizer
fight the visible signs of environmental
protection against toxic
helps chemicals,
to neutralize the effects ofUV, heavydamage
smoke, metals, cigarette
resulting from UV light, pollutants,
smog and surface radicals
smoke, fuel exhaust and urban pollution! and harmful chemicals

Clinique
Comfort On Call Allergy Tested Relief Cream
Clarins protects skin against wind and cold;
Expertise 3P Screen Mist said to prevent pollen allergies
Anti-Electromagnetic Waves &
Urban Pollution
Reality…

• Human activities
AffectCosmetics
Lucas Meyer everybody!!!
Innovations
• Urban pollution
Causefound the
loss of solution
skin in a hidden
hydration, protection,
• Sun exposure paradise
dull skin of Frenchskin
complexion, Polynesia…
sensitivity…
• Cigarette smoke
French Polynesia a hidden beauty beyond the
ordinary…
French Polynesia are volcanic islands and atolls located in
the eastern South Pacific

Moorea Island called the «Magical Island» is an atoll

Atoll is an island of coral that encircles a lagoon

Volcano

Corals that grow on the flanks of

the volcano
Exotic ExoPolySaccharides
The salty water of Moorea lagoon are the site of Kopara

Kopara are unique ecosystem constituted of microorganisms only

To protect themselves from UV, pH & salinity variations, Kopara

microorganisms produce exopolysaccharides (EPS) as protective shield

What is an exopolysaccharide vs polysaccharides?!?

Polysaccharides: Polymers that are composed of repeated sugar.
Example of polysaccharide: cellulose, chitine, cell membrane constituants of yeast

Exopolysaccharides: Polymers that are composed of sugar and are secreted

by a microorganism into the surrounding environment.

Lucas Meyer Cosmetics has isolated mircoorganisms producing EPS:

Vibrio alginolyticus subspecies 245 (Exo-T) Exclusive & Patented!
Alteromonas macleodi subspecies 203 (Exo-H)
Alteromonas macleodi subspecies 229 (Exo-P)
Sustainable ExoPolySaccharides
EPS are obtained through a proprietary green fermentation process

This process allows us to reproduce the Kopara ecosystem

without damaging the natural resources & disrupting the
biodiversity

EPS are produced in French Polynesia

to help maintain the local economy
The Exossine System:
Protection - Hydration - Texture
 Exo-P™ Product composition

Exo-P is formulated in butylene glycol and water without preservative

Exo-P 1000 kDa

Protection Produced by Alteromonas macleodi subsp 229
Ramified polysaccharide composed:
-57% neutral sugars (glucose, galactose, mannose)
-25% uronic acid (glucuronic acid, galacturonic acid)
-8% sulphates

EPS are rich in essential minerals like: sodium, potassium, calcium & magnesium

Sodium: Detoxification & maintenance water balance

Potassium: Balances healthy skin water balance
Calcium: Protects skin tissues, induces cell differentiation for a better skin barrier function
Magnesium: Stimulates cell regeneration, energy production and is an anti-stress agent
 Exo-P
the Natural Protector
In vitro & Ex vivo tests

- Confidential -
 Heavy metals chelation process
Chelation:
The use of chelating agents to detoxify dangerous metal by converting them to a
chemically inert form that can be eliminated

Chelating agent

Heavy Metal

Heavy metal chelation =

Heavy metal no longer toxic & cannot penetrate the skin
Effect on heavy metals chelation

Test Protocol:

• Exo-P was incubated with heavy metals Cadmium (Cd) or Lead (Pb) for 3hrs

• Centrifugation

• Concentration in Cd and Pd in supernatant by flame atomic absorption spectrometry (FAAS)

Cadmium is known to cause DNA damages and has been associated with alopecia
(hair loss) and serious skin rash

Lead increases oxidative stress and skin lipid peroxidation

Effect of Exo-P on heavy metals chelation

Cadmium Lead
180
q Cd absorbé (mg/g d'EPS)

160 300

140 250

q Pb retenu (mg/g d'EPS)

120
200
100
80 150
60
100
40
20 50

0 0
0 100 200 300 400 500 600 0 100 200 300 400 500 600
[Cd] eq (mg/L) [Pb]eq (mg/l)

Exo-P has the ability of chelating cadmium and

lead

Exo-P protects against toxic chemicals and thus limits skin

damages caused by pollution
Free Radical Cascade

UV
Stresses (internal / external)

Pollution

free-radicals production (ROS)

DNA Lipid peroxidation Collagen fibers Proteins oxidation

alterations degradation

Skin Aging
Effect on UV-induced lipid peroxidation

Test Protocol:
• Human keratinocytes incubated with Exo-P or positive control (BHA) for 24hrs
Butylated hydroxyanisole (BHA) is a well-known antioxidant added to foods to preserve
fats

• Cells were irradiated with UVB

• Lipid peroxidation is identified with a specific fluorescence probe and quantified by

cytometry
Effect on UV-induced lipid peroxidation

Exo-P reduces lipid

peroxidation in a dose-
dependant manner

Exo-P protects and prevents UV-induced skin damage to limit

premature aging caused by sun exposure
Anti-pollution effect on skin explants

Test Protocol:
• Lotion containing 3% Exo-P was applied on skin explants surface for 4 days

• On day 4 addition of filter paper containing a mixture of heavy metals & hydrocarbons (found
in fuel exhaust & cigarette smoke) on skin explants surface

Aluminium Arsenic Cadnium Chromium Mercury Nickel Lead Phosphorus Tellurium

(Al) (As) (Cd) (Cr) (Hg) (Ni) (Pb) (P) (Te)
20 10 mg/L 20 mg/L
20 mg/L 20 mg/L 2 mg/L 20 mg/L 20 mg/L 5 mg/L
mg/L

Hydrocarbons content: benzene, toluene, xylene, anthracene, naphtol

• On day 5 removal of filter paper and MDA quantification & histological observations

Malondialdehyde (MDA) = end product of lipid peroxidation used as biomarker of oxidation in

damaged skin
Anti-pollution effect on skin explants

Exo-P reduces the

production of MDA by 44%
under urban pollution
conditions

Exo-P protects against urban pollution and thus can be used to

reduce skin sensitivity and allergic reactions
caused by pollution
 Anti-pollution effect on skin explants

Control Skin + pollutant

Pycnotic nuclei = Severe cell
damage which lead to cell
death

Dermal-epidermal separation =
Lack of cohesion

Reduced collagen network =

Desease skin support
Skin + Pollutant + Exo-P™

No pycnotic nuclei Exo-P prevents the formation of

SC pycnotic nuclei and improves
Epidermis dermal-epidermal cohesion
DEJ

Dermis
Normal collagen network

Exo-P protects and reduces pollution damage on skin

 Exo-P
the Natural Protector
Consumer test
Anti-pollution consumer test
Test Protocol:
• 57 consumers (age: 30 to 50) living in a big city & 50% are smokers (29 out of 57)
• Application of a cream with 2% Exo-P in the morning & at night for a period of 7 days

Consumers agree that Exo-P makes their skin more resistant to

aggressions and improves dull skin for a healthy skin complexion
 Toxicological Studies

• Cytotoxicity (maximal dose: 30%)

• HET CAM (maximal dose: 30%)

• AMES Test (maximal dose: 30%)
• Acute skin tolerance (single patch test) (maximal dose: 30%)
• HRIPT (irritation and sensitization) (maximal dose: 30%)
• Photosensitization (maximal dose: 30%)

Excellent Safety Profile

Products Efficacy Summary
In vitro Ex vivo Clinical

Exo-P -Heavy metals -Protection against Anti-pollution consumer test

chelation pollutants (cigarette -Improves skin dullness,
Protection
-Protection against smoke & fuel resistance to aggressions &
UV-induced lipids exhaust) climatic changes for a
peroxidation luminous & healthy skin
Exo-H -Stimulation of -Protection against
hyaluronic acid delipidation
Hydration
secretion -Induction of
-Induction of total lipid filaggrin expression Combined test:
synthesis (fast action! 3hrs)
-Induce skin hydration
Exo-T -Induction of -Protection against -Improve skin texture
desquamation markers delipidation
Texture
with higher efficacy -Induction of
than retinoic acid transglutaminase New results
coming soon!
-Induction of skin expression
regeneration

Complete protection & moisturizing and restructuring effect leading

to a healthy, smooth & radiant skin complexion
Products Efficacy Summary

Exo-PTM Exo-TTM
Protects against
free radicals, toxic Stimulates
chemicals, heavy metals desquamation and
and urban pollution differentiation markers
and induction of TGK
expression for maximal
skin renewal

Exo-HTM
Boosts natural
hydrating functions by
stimulating hyaluronic
acid, fillagrin and lipids
Exossine™ Applications
Super Detoxifying serum
Recommended dosage: 1-3%
2% Exo-P: anti-pollution effect
1% Aldavine: anti-inflammatory
Potential Applications: 0.01% EUK-134: neutralizes free radical
•Moisturizing care
•Detox skin care Anti-Pollution Daily Cream
•Anti-stress care 2% Exo-P: pollution protection
•Protective care 1% Exo-H: moisturizing effect
0.5% Homeo-Shield: shielding effect
•Daily care
•Anti-aging care
•Skin repair treatment Purifying Anti-Pollution mask
•Sun care 1% Exo-P: detox & improves dullness
•Body care 1% Exo-T: refines skin texture
1% CW: calms the skin
•Cosmeceutical care
•Spa care Super sensitive skin cream
•Men care 2% Exo-P: protects against urban pollution
•Hair care 0.3% Neutrazen: reduces sensitivity threshold
•Urban care 0.5% Abyssine: calms the skin
Formulation Tips
Exo-P is an aqueous gel easy to work and formulate with

Exo-P can be added a room temperature or in the cooling phase at the end of
the formulating process

Exo-P can easily be incorporated in

various aqueous formulas (emulsion O/W,
lotion, foaming base, etc...)

Exo-P will add a soft, light and creamy

touch to the formula.

No known incompatibilities

pH: 4 - 8
Exossine™ Features & Benefits

Features Benefits
Actives produced by microorganisms using a Offer Sustainable & Eco-friendly actives
green fermentation process
Supply Renewable source of ingredients

Exotic exopolysaccharides rich Give the opportunity to explore new

in minerals marketing avenues

Multi-functional (protection & improve

formulating properties)

Protective effect of Exo-P Provides complete protection against urban

pollution and UV

Positive consumer perception after only 7 Makes the skin more resistant to agressions
days and improves dull skin for healthy & luminous
skin
 Dosage
The Competition
Origin

Company / Origin Application In vitro / Ex vivo Clinical %

Product
Lucas Meyer Exopolysaccharide Skin protection Cadmiun & lead chelation 2% Exo-P: ↑ skin protection & 1-3%
Cosmetics / obtained by Kopara resistance = luminous, healthier &
microorganisms from Anti-pollution 0.1%: ↑ protection against UV
Exossine™ French Polynesia Skin radiance 3%: ↑ protection against fuel exhaust radiant look (7 days)
Exo-P & cigarette smoke (ex vivo)

Alpaflor/ Mix of plant extracts Treatment Anti-oxidant 5%: ↑ moisturization (corneometry)

Nectapure against city 3-5%
stress
L. Serobiologique Seed from Indian tree Anti-pollution Protection from cadmium & mercury Skin cleansing: +62% vs placebo
/ 3% : protects from cigarette smoke 1-3%
Purisoft
L. Serobiologique Wheat seed extract Protection from 0.1 - 0.3 - 1%: ↑ cell growth 1-3%
/ urban pollution Protects against exhaust gas
Egyptyl
Sederma / Biotech product Anti-aging 3%: ↓ peroxidation & protection from 3% ↑ protection from UVA 3%
Venuceane obtained from lipid peroxidation
bacterial Anti-oxidant 3% ↓ peroxidation
fermentation 3%: protect DNA from UVA 3% ↓ TEWL & ↑ hydration (56d)
(enzymes cocktail) 5% ↑ hydration & texture (6mths)
Solabia / Polysaccharide Second skin 5%: ↑ cytoprotection from metal 1%, 3% & 5%: protection from carbon
obtained by bacterial (protection) (cadmium) particles
Glycofilm fermentation 3-5%
3% & 5%: boost SPF
Sederma / ivy, sunflower and rice Protects from 5%: ↓ citotoxicity to cigarette smoke
bran on an acrylic
Osmopur polymer & polyol base pollution 3%: ↑ protection against exhaust
fumes & heavy metals (cadmium)
Seppic / Manoliva Olive extract Anti-pollution 6ppm: ↑ protection from cigarette 3%
+manganes gluconate smoke & peroxidation (↑ than vit C)
0.024%: anti-oxidant Fast action
Exossine™ Pricing

Exo-P: Retail: 209$/kg Retail: 141€/kg (2.82 €/kg)

Distributor: 156$/kg Distributor: 105€/kg
Distributor price Lanacityn PF : 138$/kg - 95€/kg
Exo-H: 202$/kg - 134€/kg
Exo-T: 236$/kg - 157 €/kg

What you get:

New & exotic polysaccharides
Innovative and patented product
Well defined active obtained through green process (fermentation)
Low recommended dosage range: 1 to 3%
Fast results (after 7 days)
Exo-P™ Summary

Exo-P™ is an innovative
active providing complete protection

Innovative & unique actives from exotic ecosystem

(we do not harm the local environment)

Provides complete protection

Complementary action with clinically proven efficacy to

induce long-term hydration & improve skin texture for a
smooth skin and radiant complexion!