Sequencing Report

Order number: SD170829089

Sample name Sequencing result Possible reason Suggestion
2016207_ITS_ITS_F 2 2-6
Handler: Tiffany Chen Ext: 137

Sequencing Possible problem Suggestion

result
(1-1) Insufficient DNA concentration. (1-1a) Please increase DNA concentration.
(1-2)Wrong label of primer (1-2a) Please check primer concentration.
concentration.
(1) No (1-3) No priming sites (1-3a) Please check the accuracy of the primer.
signal (1-4a) Please check the construction was successful
or not.
(1-4) A failure cloning.
(1-4b) Please check the vector has been modified or
not.
(2-1a)Please re-design a new primer (Tm = 50℃ to
(2-1) Low specificity of primer 55℃).
(2-2) Insufficient DNA concentration (2-2a) Please increase DNA concentration.
(2-3) Primer degradation (N-1, N-2..) (2-3a)Please check the storage condition of primer,
or using a fresh dissolved primer instead
(2-4a) No single product, please purify the products
(2-4) Multiple templates again.
(2) Signal (2-4b) Please choose a single colony.
disorder (2-5a) Please re-design a primer having only one
(2-5) Multiple priming sites binding site.
(2-6) Fragment shift mutation (SNP, insertion or deletion).
(2-7a) Please remove inhibitors completely among
(2-7) Low quality of sample. sequencing reaction (ex: salt, phenol, EDTA,
etc.)
(2-8a) Please re-prepare the samples and check it
(2-8) Sample smear on the gel
(3-1a) Please use a new primer from another side.
(3-1) Homopoly (T),(A),(G),(C) (3-1b)Please design a new primer to avoid this
(3) Signal effect.
interrup
t (3-2) Secondary structure (3-2a) Please use a new primer from another side.
(Repeat sequence, hair pin, (3-2b) Please use structure-releasing reagents.
siRNA..) (dGTP, DMSO)

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