S162 Abstracts / New Biotechnology 33S (2016) S1–S213
with 5.00 mg l−1 BAP. Maximum dry weight was obtained in free-
PGRs medium. About 98% of the micropropagated plantlets were
established successfully in plastic pots filled with a mixture of peat
and perlite (1:1 ratio). Acclimatized plants were morphologically
similar to the mother plants.
http://dx.doi.org/10.1016/j.nbt.2016.06.1280
P18-29
Extraction of CBD and 9-THC from three varieties of fibre hemp
by different concentrations of ethanol
Milena Szalata ∗ , Karolina Wielgus
Institute of Natural Fibres & Medicinal Plants, Poland
Hemp (Cannabis sativa L.) is an important medicinal plant.
Identification of cannabinoids such as 9-tetrahydrocannabinol
(9-THC) and cannabidiol (CBD) is important for determination
of hemp type and to control the quality. The main cannabinoid
in fibre-type hemp is CBD, which is characterized with non-
psychotropic properties. The aim of the work was the extraction
of CBD and 9-THC from fibre-type hemp varieties A, B and D.
The study focused on extraction of C. sativa panicles with different
ethanol concentration. It was found that there was considerable
variation between the extracts. The hemp variety A have signifi-
cantly higher amount of CBD (334.10–456.02 ␮g/ml) and variety D
was characterized by lower content of CBD (112.89–122.83 ␮g/ml).
The 9-THC content ranged from 27.60 to 12.19 ␮g/ml. Compari-
son of different extraction methods revealed that the concentration
of ethanol strongly affects the composition of obtained extracts.
The extraction with the high ethanol concentration (96% and 80%)
resulted in obtaining extracts with significantly higher amount of
CBD (537.13 and 330.09 ␮g/ml respectively). The amount of 9-
THC was also higher (37.66 and 42.93 ␮g/ml respectively) than in
extracts obtained using lower concentration of ethanol (0.14 ␮g/ml
and 2.52 ␮g/ml). The results have shown that the extraction of
hemp variety A is most efficient. The most effective cannabinoids
extraction was achieved using high concentration of ethanol.
Presented work was financially supported by The National
Centre for Research and Development (grant number
INNOMED/I/11/NCBR/2014) from the Innovative Economy Oper-
ational Programme funds, in the framework of the European
Regional Development Fund.
http://dx.doi.org/10.1016/j.nbt.2016.06.1281
P18-30
Evolution and production platform for phytochemicals derived
from plant cell cultures
Christian Schmitz ∗ , Leonie Fritsch, Stefan Rasche
Maastricht University; Aachen-Maastricht Institute for Biobased
Materials [AMIBM]
Plant cell cultures are biological refineries for the production
of phytochemicals, secondary metabolites and heterologous pro-
tein expression. Several advantages in comparison to greenhouse
grown plants are observed: 1) Plant cell cultures are indepen-
dent on seasonal changes, 2) have a higher up-scaling potential,
3) can be cultivated under constant conditions, which leads to con-
stant product quality 4) only desired tissue with highly abundant
target compound accumulation is cultivated 5) are a better subjects
concerning optimization studies.
In our project we desire to produce valuable secondary metabo-
lites with plant cell and tissue cultures either for direct use
(fragrances, pigments, antioxidants) or the production of novel
biobased polymers (building blocks). To increase the economic
impact of the desired compounds, the productivity of plant cell
cultures must be maximized. Our aim is to improve the establish-
ment of plant tissue cultures with a novel Design-of-experiments
(DoE) approach to investigate the influence of different spectral
light and phytohormones on the callus induction. Further process
optimization is performed on plant cell cultures to improve the
biomass production and to enhance the target metabolite accumu-
lation. Therefore, we use one DoE approach for the optimization
of physical cultivation conditions (temperature, light, humidity).
Additionally, medium optimization is performed by a mixture DoE.
Commercially available and commonly used media (e.g. Gamborgs
B5, and Murashige & Skoog) are blended in different ratios to inves-
tigate where maximum productivity of the cells is achieved. With
improved cultivation conditions and product yield, we aim to enter
medium scale production and downstream-processing of the target
metabolites.
Keywords: Plant Biotechnology, Plant cell and tissue cultures,
Secondary Metabolites, Design of Experiments, Production opti-
mization
http://dx.doi.org/10.1016/j.nbt.2016.06.1282
P18-31
The nutrient composition of northern berries
Olga Dymova 1,∗ , Ekaterina Lashmanova 2 , Galina Tabalenkova 3 ,
Ilya Zakhozhiy 4 , Tamara Golovko 1
1 Institute of Biology, Russia
2 Komi Science Center, Russia
3 Ural Branch of RAS, Russia
4 Moscow Institute of Physics and Technology (State University),
Russia
Cloudberry (Rubus chamaemorus), blueberry (Vaccinium myr-
tillus), cowberry (Vaccinium vitis-idaea) and cranberry (Oxycoccus
palustris) are the main wild-growing berries in the European north-
east of Russia. Biological resources of these berries exceed 300
thousand tons in taiga zone of the Komi Republic. The value
of berries is determined by their beneficial effects on human
health. For example, cloudberry, blueberry and cranberry extracts
increased lifespan and stress resistance of Drosophila melanogaster
and Caenorhabditis elegans. Northern berries are rich in carotenoids
(Car) (lutein, zeaxanthin and ␤-carotene) as well as pheno-
lic compounds, soluble carbohydrates, organic and amino acids,
and dietary minerals. Among studied berries the cloudberry and
blueberry fruits contented the substances with the highest bio-
logical activity. Cloudberry was rich in ␤-carotene (82% of total
Car), blueberry – in lutein (over 70% of total Car). The con-
tent of phenolic compounds in fruits varied between 17 and
34 mg GAE/100 g of dry weight (DW) and was highest in blueberries
and cowberries. The berries were placed in the order blue-
berry > cowberry > cloudberry > cranberry on the content of soluble
sugars (215–320 mg/g DW). Fructose and glucose (97–99%) pre-
dominated among the soluble sugars. The organic acids content
varied from 1.7 to 10.6%. The berries contained a high amounts
of dicarboxylic acids (glutamic and aspartic), which together with
arginine, leucine, glycine, lysine and proline made up to 70% of the
total free amino acids. Biotechnological approaches need to be used