Relative toxicity of some newer molecules against Rice Moth

[Corcyra cephalonica (St.)] under laboratory condition

Arunabha Chakraborty and Sudarshan Chakraborti
1UniversityResearch Scholar and 2Reader, Department of Agricultural Entomology,
Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia-741252 (W.B.) India.
Contact email id: arubckv@gmail.com

The Epilogue:
o Worldwide sustainability of agriculture o Rice moth Corcyra cephalonica (Stainton, 1865) is a serious
suffers a severe setback arising from problem in some rice producing belts and beside this, larvae of
the use of chemical pesticides on living rice moth is one of the most used factitious host for
systems and the environment Trichogramma production in the several countries of the world
1. Excessive spraying in rice field

o The Indian farmers too are in need of o As the avermectins, a streptomycete-derived

effective tools to fight against pests. On
account of above the farming
community needs safer, effective and
economical insecticides
2. Lab rearing of Corcyra cephalonica (St.)
lactones, Azadirachtin (10,000 ppm) and Indoxacarb are three
new generation formulations and known for their specific action
against lepidopteran larvae, we carried out some experiments
3. Adult of Corcyra cephalonica (St.)
about their toxicity on rice moth Corcyra cephalonica (St.)

The Objectives:
o Particular LC50 value for each chemical against rice moth larvae at specific time interval
o Oder of relative toxicity of the used three insecticides against rice moth larvae
o Specific mortality percentage of the larvae at different concentrations of insecticides
Methodology:
o Moths are directly collected from field into both end net closed handmade oviposition cages
o Eggs are collected from the base Petridish by a fine and pointed brush and in a small vial
o A cheaper diet (wheat flour : crashed corn in 5:4 ratio) was used to rear the larvae in laboratory
o Culture was maintained in some large glass containers at 28 ±1oC and 90 ± 5 % R.H.
o Indoxacarb 400, 200 and 100 ppm solution, Abamectin 200, 100 and 50 ppm solution and
Azadirachtin 10000, 500 0and 2500 ppm solution were prepared by mixing Indoxacarb 14.5%
SC, Abamectin 1.9% w/w EC and Neemacin respectively with distilled water
o 3rd instar larvae of Corcyra cephalonica (St.) were treated with each different concentration by
topical application with the help of hand atomizer
o LC50 values were calculated for 24, 48 and 72 h by method described by Finney (1952) and
simplified by Busvine (1971) 1. Rice moth larvae ready for experiments
and 2. larvae under treatment in laboratory
Important results:
The percent mortality at various doses of insecticides is subjected to probit analysis. The LC50 values, heterogeneity values with degrees of
freedom, regression equation and fiducial limits, relative toxicity and order of relative toxicity were obtained and those are as follows:
LC50 Fiducial Limit Relative Order of
Hrs. Insecticide df ?2 value Regression Equation (in ppm) (in ppm) Toxicity Relative
Lower Upper Toxicity
Indoxacarb 14.5% SC 1 1.085 Y = 1.6597 X – 5.0396 1087.81 634.24 5004.89 5.22 2

24 Abamectin 1.9% w/w EC 1 0.003 Y = 1.3841 X – 2.9887 144.30 112.83 215.82 39.38 1

Neemacin 1 0.607 Y = 1.6511 X – 6.1991 5681.99 4619.23 7232.62 1 3

Indoxacarb 14.5% SC 1 0.064 Y = 0.8218 X – 2.7006 1933.44 684.23 2548.64 1.37 2

48 Abamectin 1.9% w/w EC 1 0.411 Y = 1.5799 X – 2.6536 47.80 28.39 62.67 55.55 1

Neemacin 1 0.024 Y = 1.8027 X – 6.1727 2655.45 1811.35 3327.77 1 3

Indoxacarb 14.5% SC 1 1.649 Y = 1.5687 X – 2.7044 52.96 18.63 880.93 21.63 2

72 Abamectin 1.9% w/w EC 1 0.269 Y = 1.5854 X – 1.5581 9.16 0.21 22.64 119.22 1

Neemacin 1 1.564 Y = 2.5987 X – 7.9495 1145.71 423.25 1682.68 1 3

Observations: Summury:
Freshly emerged 3rd instar larvae were more sensitive • The relative toxicity values (24 hours) were 39.38 and 5.22 for Abamectin
Percentage survival rate of the larvae decreased with and Indoxacarb taking Neemacin as unit due to its highest LC50 value
increasing concentration of the insecticides
It is evident from the result table that the acute toxicity (24
• Relative toxicity at 48 hours was 55.55 and 1.37 for Abamectin and
Indoxacarb
hours) of Abamectin 1.9% w/w EC was highest (LC50 value
144.30 ppm) to the 3rd instar larvae of Corcyra cephalonica • At 72 hours those values was 119.22 and 21.63 respectively
(St.) during the course of investigation and the mortality • In a descending order of relative toxicity we found Abamectin 1.9% w/w
percentage was as high as 57.89% (in 200 ppm application) EC at the top followed by Indoxacarb 14.5% SC and Neemacin at second
References:
and third place respectively
Busvine, J.R., 1971, Critical review of the technique for testing insecticides. • From overall result and order of relative toxicity, Abamectin 1.9% W/W EC
Common Wealth Agriculture Bureax, England, 267-282 pp. was found to be most effective against Corcyra cephalonica (St.) larvae
Finney, D.J., 1952, Probit analysis, Cambridge University, Press, 2nd Edn.: 318 pp. under laboratory condition