LUNA ®

One of the most popular HPLC columns Material Characteristics

Packing Particle Pore Surface Carbon Calculated Bonded
worldwide Material Shape/Size Size Area Load Phase Coverage
• pH stability from 1.5 to 10.0* for over 1000 hours (µ) (Å) (m2/g) % (µmole/m2)
Luna Silica(2) Spher. 3, 5,10,15 100 400 0 –
• Extensive batch traceability data provided with Luna C5 Spher. 5,10 100 440 12.5 7.85
every 3µ and 5µ column Luna C8 Spher. 5,10 100 440 14.75 5.50
Luna C8(2) Spher. 3, 5,10,15 100 400 13.5 5.50
• Extreme versatility of 3, 5, 10, 10-PREP and 15µ particles
Luna C18 Spher. 5,10 100 440 19 3.00
and bulk material availability Luna C18(2) Spher. 3, 5,10,15 100 400 17.5 3.00
• Phases available include: Si, C5, C8, C18, Luna Phenyl-Hexyl Spher. 3, 5,10, 15 100 400 17.5 4.00
Phenyl-Hexyl, CN, NH2 and SCX Luna CN* Spher. 3, 5, 10 100 400 7.0 3.80
Luna NH2* Spher. 3, 5, 10 100 400 9.5 5.80
Luna SCX* Spher. 5,10 100 400 0.15 meq/g
Sharp Peak Shape

HPLC
®
Luna is manufactured with remarkable silica, dense bonded
phase coverage and exhaustive endcapping to improve peak Peak Asymmetry Comparison of Competing Columns**
shape over a broad pH range for accurate, sensitive meth-
®
ods. Free exposed silanols contribute significantly to peak LUNA 5µ C18(2)
tailing. Incomplete or lack of endcapping exposes free silanols
to the analytes, and additionally metal contamination will in-

Luna
crease the acidity of the free silanols, leaving them in a 1
400 Pyridine Asymmetry:
charged state even at low pH. Using a simple 50:50 water/ As 1.44
2
acetonitrile mobile phase, the peak shapes and separation 300
of pyridine and phenol provides a good indicator of free
200 3
silanols (see results below). Under these conditions, in the
presence of ionized exposed silanols, pyridine elutes as a 100
tailing peak, typically coeluting or even eluting after phenol. 0
0 2 4 6 8 10 12 min

Pyridine Peak Asymmetry Comparison**

2 ®
Symmetry 5µ C18
300

1 Pyridine Asymmetry: 3
As 2.27
200

100

0
0 2 4 6 8 10 12 min

®
2 Zorbax 5µ C18
500
Pyridine Adsorption:
400 No elution

300 3

200

100 1

0
Comparison of 7 different 5µ reversed phase columns. This
0 2 4 6 8 10 12 min
survey measures the degree of silanol activity on the surface
of each silica. In this survey, Luna 5µ C18(2) material demon-
strates the lowest silanol activity. Conditions for all columns
App ID 1100

Dimensions: 150 x 4.6mm

*Luna CN is stable from pH 1.5 to 7.0, Luna Amino (NH2) is stable from pH 1.5 to 11.0, Mobile Phase: Acetonitrile/Water (50:50)
Luna SCX is stable from pH 2.0 to 7.0. Flow rate: 1.0mL/min
**The comparative data presented here may not be representative for all applications. Detection: UV @ 254nm
Sample: 1. Pyridine
2. Phenol
3. Toluene

Phenomenex 143
 LUNA ®

Remarkable Silica Technology Dense Uniform Bonding

Luna’s base silica is manufactured to extremely tight toler-
ances. Not only is it 99.999% ultrapure, but it has excellent
surface smoothness and sphericity. The structural stability
of individual silica particles and stability of the column bed
are enhanced by improved silica smoothness. The strength
of Luna silica reduces the likelihood of particle sheering and
breakage during bonding and everyday column use, thus
reducing the number of “fines”, lowering backpressure, and
lengthening column life.
Compare Surface Smoothness
HPLC
Luna columns employ bonding technology that provides ex-
tremely high surface coverage not only through high-bonding
densities, but also controlled bonding uniformity. This high
degree of uniformity forms a more inert “hydrophobic shield”
around the underlying silica. The result is a pH stable, repro-
ducible bonding with virtually no silanol activity.
Scale-up Diversity
Luna is available in 3, 5, 10, 10-PREP and 15µ particle sizes
as packed columns or bulk media for fast, simple scale up.
Your methods will have scale-up flexibility without the need to

redevelop on another sorbent.

Quality Proven
Extensive testing and quality control test data is shown
on the Materials Validation Document (MVD) that accom-
panies each 3 and 5µ Luna column. Over 25 individual
chemical and physical testes are performed and docu-
Luna

mented on the MVD, including:

• Particle Analysis
- Particle Size
Phenomenex Luna®

- Pore Diameter
- Particle Size Distribution
- Surface Area
• Total Metal Content
• Bonded Phase Coverage
5µ C18

- Total Carbon
- Surface Coverage
• Diagnostic Chromatography Test
- Inertness
- Metal Sensitivity
- Hydrophobic Index
• Performance Chromatography Test
- Basic Drug Standards
- Longevity Tests (pH Stability)
- Scanning Electron Microscopy (measures
surface smoothness and particle shape)
Waters Symmetry®
5µ C18
Agilent Technologies Zorbax®
5µ SB-C18

144 Phenomenex
 LUNA ®

If Luna does not provide at least an

equivalent separation as compared to a
competing column of the same particle
Wide pH Stability size, similar phase and dimensions, send
in your comparative data within 45 days
Luna’s 1.5 to 10 pH stability** is a result of manufacturing high and keep the Luna column for FREE.
purity silica that is nearly free of metal contaminates. Metal
contaminants hinder maximum bonded phase coverage of the
silica surface, which is necessary for achieving a broad pH
stability range. Our bonding technology ensures the bonded
phase coverage is extraordinarily dense creating a “hydropho-
bic shield” around the silica.

pH Stability Data for Luna 5µ C18(2)

HPLC
Luna
Test Conditions: Column stability tested under highly acidic conditions.
Continuous flush in 0.1% TFA (pH 1.5) in Water/Acetonitrile, 50:50.

Test Conditions: Column stability tested under highly basic conditions.

Continuous flush in 20mM Na2HPO4 (pH 10.0) in Water/Acetonitrile, 50:50.

KEY:
Efficiency, Plates for Toluene
tR of Toluene (min)
k of Toluene
Symmetry of Toluene
s Symmetry of Pyridine

** Luna CN is stable from pH 1.5 to 7.0

Luna NH 2 is stable from pH 1.5 to 11.0
Luna SCX is stable from pH 2.0 to 7.0.

Phenomenex 145
 LUNA ®

Chromatographic Comparisons of C18 Columns*

App ID 12903 App ID 12904
®
LUNA 3µ Waters Symmetry 3.5µ C18 ®

C18(2) 175
1
4

150
125 2
5
100 3
75 6
50
25
0

0 5 10 15 20 min

®
Agilent Technologies Zorbax 3.5µ
HPLC

Anti-Inflammatory/Analgesic Drugs SB-C18

3
Conditions for all columns: 175 4
Dimensions: 75 x 4.6mm 150

Mobile Phase: 20mM KH2PO4, pH 6.0/Acetonitrile (70:30) 125

1
2
100
Flow rate: 0.75mL/min 75 5
6
Detection: UV @ 202nm 50

Sample: 1. Tolmetin 4. Fenoprofen 25

Luna

0
2. Naproxen 5. Indomethacin 0 5 10 15 20 min
3. Diflunisal 6. Ibuprofen
App ID 12906

Chromatographic Comparisons of C8 Columns*

App ID 12967 App ID 12908 App ID 12909

1,2,3
3 1400

1000
1200
2 5
800 1000
5
800
600
1 4 600 4
400
400
200
200

0
0
1 2 3 4 5 mi n
1 2 3 4 5 min

®
LUNA 5µ C8 Waters Symmetry 5µ C8
®
Waters SymmetryShield
™

5µ RP8
1 1
Tricyclic Antidepressants 700

Conditions for all columns: 600 200

Dimensions: 150 x 4.6mm
500
Mobile Phase: 10mM KH2PO4, pH7.0/Acetonitrile/ 3
3 150
Methanol (16:74:10) 5
400 5
Flow rate: 1.0mL/min
300 4 100
Detection: UV @ 210nm 2
2 4

Sample: 1. Desipramine 200

2. Nortriptyline 100
50

3. Doxepin
4. Imipramine 0 0

5. Amitriptyline 2 4 6 8 min 5 10 15 min

Agilent Technologies Agilent Technologies

® ®
Zorbax 5µ XDB-C8 Zorbax 5µ SB-C8
App ID 12910 App ID 12911

*The comparative data presented here may not be representative for all applications.

146 Phenomenex
 LUNA ®

Chromatographic Comparisons of Phenyl Columns*

PHENYL-HEXYL ®
(patented) 6
LUNA 5µ
7
• Offers greater stability over common propyl- Phenyl-Hexyl
linked phenyl phases
• Selectivity benefits of both a phenyl and alkyl 3
2
phase combined 5 8

App ID 1201
1 4
• Improved stability - pH 1.5 to 10.0 for over 2000 hours

Luna Phenyl-Hexyl employs a hexyl-linked phenyl phase. The

hexyl alkyl chain linker offers remarkable selectivity and greater
5 10 15 20 min
hydrolytic stability over the common propyl-linked chemistry.

HPLC
Luna Phenyl-Hexyl offers the chromatographer a high level of
performance and durability along with excellent selectivity.

®
pH Stability of Luna Phenyl-Hexyl Waters Spherisorb 5µ
Phenyl

Luna
Efficiency of Toluene pl/col

14000

App ID 10822
12000

10000
8
pH 1.5
tR, k, Symmetry of Toluene and Pyridine

7 Agilent Technologies
6 ®
Zorbax 5µ
5
SB-Phenyl
4

2
App ID 10821

0
0 24 48 72 96 168 336 504 624 1008 1368 1632 1776 2256 2472

Hours of Exposure

14000
Efficiency of Toluene pl/col

Agilent Technologies
®

12000
Zorbax 5µ

10000
8
pH 10.0 Phenyl
App ID 10823

7
tR, k, Symmetry of Toluene and Pyridine

2 Antibacterials
1 Conditions for all columns:
Dimensions: 150 x 4.6mm Sample: 1. Carbadox
0 Mobile Phase: A: 20mM KH2PO4, pH 2.5 2. Thiamphenicol
0 24 48 72 96 168 336 504 624 1008 1368 1632 1776 2256 2472
B: Acetonitrile 3. Furazolidone
Hours of Exposure Gradient: Start A/B (80:20) to A/B (75:25) in 4. Oxolinic Acid
5 min, go to A/B (55:45) in 15 min 5. Sulfadimethoxine
KEY: Flow Rate: 1.0 mL/min 6. Sulfaquinoxaline
Efficiency, Plates for Toluene Detection: UV @ 254nm 7. Nalidixic Acid
8. Piromidic Acid
tR of Toluene (min)
k of Toluene
Symmetry of Toluene
s Symmetry of Pyridine
*The comparative data presented here may not be representative for all applications.

Phenomenex 147
 LUNA ®

AMINO (NH2) CYANO (CN)

Luna Amino (NH 2 ) was developed to improve column rugged-
ness and reproducibility. What results is a unique amino, stable
from pH 1.5 to 11.0, in 100% aqueous mobile phases, and in
reversed and normal phases. For mono- and oligosaccharide
analysis or for any normal phase separation Luna Amino is an
excellent choice.
Luna CN shows remarkable performance and reproducibility.
Luna’s revolutionary base silica has exceptional surface smooth-
ness and consistency and minimizes variations during the bond-
ing process. The result is a CN phase with excellent performance
and reproducibility batch-to-batch and column-to-column.

Testing Results for 3 Luna Amino Batches Testing Results for 3 Luna Cyano Batches

Batch 1
HPLC

App ID 14320
Luna

Batch 1
App ID 3464

Batch 2
Batch 2
Batch 3

Column: LUNA® 5µ Cyano

Dimensions: 150 x 4.6mm
Mobile Phase: A=Hexane, B=Methylene chloride/
Methanol(80:20)
App ID 14321

A/B (80:20)
Flow Rate: 2.0 mL/min
Detection: UV @ 254nm
Injection volume: 1.0 µL
Temperature: Ambient
Sample: 1. Hydrocortisone
2. Prednisone
3. Cortisone
4. Hydrocortisone Acetate
Batch 3
App ID 14322

Column: Luna 5µ NH2 100Å

Dimensions: 250 x 4.6mm
Order Number: 00G-4378-E0
Mobile Phase: Acetonitrile/Water (80:20)
Flow Rate: 3.0mL/min
Detector: RI
Temperature: 40°C
Injection Volume: 10µL
Sample: 1. Fructose (0.7mg/mL)
2. Glucose (0.75mg/mL)
3. Sucrose (3mg/mL)
4. Maltose (0.75mg/mL)

148 Phenomenex
 LUNA ®

STRONG CATION EXCHANGE (SCX)

Luna SCX is a silica-based sorbent derivatized with benzene sulfonic acid phase to selectively separate amine-
containing and cationic species from a mixture of neutral and anionic compounds. Conveniently packed with 5µ ÿ for
analytical separation and 10µ ÿ for scale-up, Luna SCX material has been shown to be reproducible and to have an
operating pH range between 2.0 to 7.0.

Luna SCX vs. Competition Luna C18 vs. SCX

4
3
1 ®
LUNA SCX
140 2 ®

200
LUNA SCX
5µ 100Å 120

5µ 100Å
100 1

80
3
mAU
100

HPLC
App ID 14553
App ID 14547

60

2
mAU 5
40
4

6 20

0 2 4 6 8 10 12 min 0

0 2 4 6 8 min

Luna
3 ®
100
400
1

2
LUNA C18(2)
®
Waters Spherisorb SCX 5µ 100Å
80
1
5µ 80Å 300

mAU
5
60
200 3
mAU
App ID 14554
App ID 14548

4 4
40
2
100

20
6

0 0 2 4 6 min

0 2 4 6 8 10 min

Macherey-Nagel Oxycodone Derviatives

1

Nucleosil SA ®
Conditions for all columns:
80 Dimensions: 150 x 4.6mm Sample: 1. Noroxymorphone
5µ 100Å Mobile Phase: 50mM KH2PO4, 2. Noroxycodone
pH 2.5/Acetonitrile (50:50) 3. Oxymorphone
60
Flow Rate: 2.0 mL/min 4. Oxycodone
mAU

40 3, 6
Detection: UV @ 210nm
App ID 14549

2 Injection Volume: 5µL

4, 5
20

0 2 4 6 8 10 12 min

1, 2

TM
Whatman Partisil SCX
200
5µ 85Å
mAU
3
App ID 14550

100

5
4

6
0

0 2 4 6 8 10 12 min

Cocaine and Metabolites

Conditions for all columns:
Dimensions: 150 x 4.6mm Sample: 1. m-Hydroxylbenzoyl Ecgonine
Mobile Phase: 50mM KH2PO4, pH 2.5 2. Norbenzoyl Ecgonine
/Acetonitrile (65:35) 3. Norcocaine
Flow Rate: 2.0 mL/min 4. Benzoyl Ecgonine Isopropyl Ester
5. Cocaine
Detection: UV @ 210nm 6. Ecgonine Methyl Ester
Injection Volume: 20µL

Phenomenex 149
 LUNA ®

Fatty Acids Steroids

App ID 1258

App ID 1185
Column: Luna 5µ C8(2) Column: Luna 5µ C18(2)
Dimensions: 150 x 4.6mm Dimensions: 150 x 4.6mm
Order No: 00F-4249-E0 Order No: 00F-4252-E0
Mobile Phase: A: Acetonitrile Mobile Phase: 0.1% H3PO4 /Acetonitrile/Methanol (54:35:11)
B: Water 7
Flow Rate: 0.75 mL/min
Gradient: A/B (70:30) to A/B (90:10) in 10 min, Detection: UV @ 254nm
to A/B (70:30) in 12 min Sample: 1. Hydrocortisone
Flow Rate: 0.3mL/min 2. Corticosterone
Temperature: 22°C 3. 11-a-Hydroxyprogesterone
Detection: Evaporative Light Scattering 4. Cortisone Acetate
Sample: 1. Lauric Acid 8 5. 11-Ketoprogesterone
6
2. Myristic Acid 1
3
3. Palmitolic Acid 1000
4. Linoleic Acid 4 5 2
HPLC

2
5. Palmitic Acid 800
1
6. Oleic Acid 3
600
7. Heptadecanoic Acid
8. Stearic Acid 400 5
4
200

0
Luna

0 2 4 6 8 10 12 14 min 2 4 6 8 10 12 14 16 min

Basic Drugs Parabens

App ID 1147

App ID 1208
Column: Luna 5µ C18(2) Column: Luna 5µ C18(2)
Dimensions: 150 x 4.6mm Dimensions: 150 x 4.6mm
Order No: 00F-4252-E0 Order No: 00F-4252-E0
Mobile Phase: 20mM KH2PO4, pH 7.0/Methanol (10:90) Mobile Phase: Acetonitrile/Water (50:50)
Flow Rate: 1.0 mL/min Flow Rate: 1.0 mL/min
Detection: UV @ 254nm Detection: UV @ 254nm
Sample: 1. Pseudoephedrine Sample: 25mg/mL of each:
2. Diphenhydramine 1. Methylparaben
3. Amitriptyline 2. Ethylparaben
3. Propylparaben
4. Butylparaben
800 1 4
160
600 2 1
120
400
3 80
2
200
40
3
0 0
1 2 3 4 5 min 0 2 4 6 8 min
App ID 12913

Water-Soluble Vitamins 4

Column: Luna 5µ C18(2) 6

Dimensions: 150 x 4.6mm
Order No: 00F-4252-E0
Mobile Phase: A: Water/Methanol/H3PO4 + 10mM sodium hexane sulfonate
(95 : 5 : 0.2)
4
B: Methanol/Water/H3PO4 + 10mM sodium hexane sulfonate
(50 : 50 : 0.2) 3
Gradient: 0% B to 100% B over 30 min
Flow Rate: 1.5 mL/min
Detection: UV @ 275nm 2
1
Sample: 1. Niacinamide
2. Pyridoxine 2
5
3. Riboflavin
4. Folic Acid
5. Thiamine 0

0 10 20 30 min

150 Phenomenex
 LUNA App ID 14289
®

Sugar Alcohols Narcotic Analgesics

App ID 1164
Column: Luna 5µ NH2 Column: Luna 5µ C18
Dimensions: 250 x 4.6mm Dimensions: 150 x 4.6mm
Order No : 00G-4378-E0 Order No.: 00F-4041-E0
Mobile Phase: Acetonitrile/Water (85:15) Mobile Phase: A: 20mM KH2PO4, pH 2.0
Flow Rate: 3 mL/min B: Acetonitrile/Methanol (80:20)
Detection: RI A/B - (85:15)
Temperature: 40°C Flow Rate: 1.0 mL/min
Sample: 1. Glycerol Detection: UV @ 220nm
2. Pentaerythritol Sample: 1. Morphine
3. Xylitol 2. Codeine Phosphate
4. Sorbitol 3. Oxycodone
5. Inositol 4. Hydrocodone Bitartrate

HPLC
1
4
2

Luna
1 2 3 4 5 min
App ID 14263

Explosives - EPA Method 8330 Cough and Cold Compounds

App ID 2578

Column: Luna 5µ C18(2) Column: Luna 5µ C8(2)

Dimensions: 250 x 4.6mm Dimensions: 150 x 4.6mm
Order No.: 00G-4252-E0 Order No.: 00F-4249-E0
Mobile Phase: Methanol/Water (55:45) Mobile Phase: A: Methanol B: Acetonitrile w/0.1% H3P04
Flow Rate: 1.0 mL/min C: Water w/0.1% H3P04 and 0.1% heptane sulfonate
Detection: UV @ 254nm A/B/C (40:10:50)
Temperature: 35°C Flow Rate: 1.5mL/min
Sample: 1. HMX Temperature: 22°C
2. RDX Detection: UV@ 214nm
3. 1,3,5-Trinitrobenzene Sample: 1. Acetaminophen
4. 1,3-Dinitrobenzene 2. Pseudoephedrine
5. Tetryl 3. Benzoic acid
6. Nitrobenzene 4. Chlorpheniramine
7. 2,4,6-Trinitrotoluene 5. Dextromethorphan
8. 4-Amino-2,6-Dinitriotoluene
9. 2-Amino-4,6-Dinitriotoluene
10. 2,6-Dinitrotoluene
11. 2,4-Dinitrotoluene
12. 2-Nitrotoluene
13. 4-Nitrotoluene
14. 3-Nitrotoluene

5 11

1
7

2 6 9

8 10

12
14
13

0 2 4 6 8 10 12 14 16

Phenomenex 151
 LUNA ®

Saw Palmeto Berry, p-Bromophenacyl Esters Antihistamines

App ID 1107
App ID 1072

Column: Luna 3µ C8(2) Column: Luna 3µ C8 (2)

Dimensions: 150 x 4.6mm Dimensions: 150 x 4.6mm
Order No.: 00F-4248-E0 Order No.: 00F-4248-E0
Mobile Phase: Acetonitrile/Water (87:13) Mobile Phase: 0.5mM K2HPO4 /
Flow Rate: 1.5 mL/min Acetonitrile (52.6:47.4)
Temperature: 25°C Flow Rate: 1.3 mL/min
Detection: UV @ 254nm Temperature: 25°C
Sample: 1. Capric Acid 5. Linoleic Acid Detection: UV @ 249nm
2. Lauric Acid 6. Palmitic Acid Sample: 1. Promethazine Sulfoxide
3. Linolenic Acid 7. Oleic Acid 2. N-Desmethylpromethazine
4. Myristic Acid 8. Stearic Acid 3. Promethazine Hydrochloride
2
4. Isopromethiazine
5. Phenothiazine
7
HPLC

2
Luna

6
1 5
4

1 5

3 8

0 10 20 25 min 0 5 10 15 20 min

Antibacterials Food Additives

App ID 1201

App ID 1199

Column: Luna 5µ Phenyl-Hexyl Column: Luna 5µ Phenyl-Hexyl

Dimensions: 150 x 4.6mm Dimensions: 150 x 4.6mm
Order No.: 00F-4257-E0 Order No.: 00F-4257-E0
Mobile Phase: A: 20mM KH2PO4, pH 2.5 Mobile Phase: A: 50mM KH2PO4 + 0.1% H3PO4
B: Acetonitrile B: Acetonitrile
Gradient: A/B (80:20) to A/B (75:25) in 5 min, Gradient: A/B (75:25) for 18 min,
to A/B (55:45) in 15 min to A/B (25:75) in 12 min
Flow Rate: 1.0 mL/min Flow Rate: 1.0 mL/min
Detection: UV @ 254nm Detection: UV @ 230nm
Sample: 1. Carbadox Sample: 1. Saccharin
2. Thiamphenicol 2. p-Hydroxybenzoic Acid
3. Furazolidone 3. Sorbic Acid
4. Oxolinic Acid 4. p-Hydroxybenzoic Acid Methyl Ester
5. Sulfadimethoxine 5. Dehydroacetic Acid
6. Sulfaquinoxaline 6. p-Toluic Acid
7. Nalidixic Acid 7. p-Hydroxybenzoic Acid Ethyl Ester
8. Piromidic Acid 8. n-Propyl p-Hydroxybenzoate

6
7

3
2 8
5
1 4

5 10 15 20 min

152 Phenomenex
 LUNA ®

Glucocorticoids Antibiotics
App ID 1284

App ID 1245
Column: Luna 5µ C8 Column: Luna 5µ C8(2)
23
Dimensions: 150 x 4.6mm Dimensions: 150 x 4.6mm
Order No: 00F-4040-E0 Order No: 00F-4249-E0
Mobile Phase: Acetonitrile/Water (50:50) Mobile Phase: Acetonitrile/Water (30:70)
Flow Rate: 1.0 mL/min 1 Flow Rate: 1.0 mL/min
4
Detection: UV @ 240nm Detection: UV @ 365nm
Sample: 1. Dexamethasone 21-Phosphate Sample: 1. Oxytetracycline
2. Prednisolone 2. Tetracycline
3. Dexamethasone
4. Prednisolone 21-Acetate mAU
2
1
5. Betamethasone 17-Valerate 25

5
20

HPLC
15

10

Luna
0 2 4 6 8 10 min 0 0.5 1.0 1.5 2.0 2.5 min

Herbicides Acetaminophen, USP Method

App ID 1260

App ID 1293

Column: Luna 5µ C8(2) Column: Luna 5µ C18(2)

Dimensions: 150 x 4.6mm Dimensions: 150 x 4.6mm
Order No: 00F-4249-E0 Order No: 00F-4252-E0
Mobile Phase: Acetonitrile/Water Mobile Phase: Water/Methanol/Acetic Acid (69:28:3)
Gradient: 70 to 100% in 3 min Flow Rate: 1.5 mL/min
Flow Rate: 1.0 mL/min Detection: UV @ 275nm
Detection: UV @ 210nm Sample: 1. Acetaminophen
Sample: 1. Tebuthiuron 2. Caffeine
2. Simazine 3. Benzoic Acid
3. Altrazine
4. Propizine 2
5. Dacthol

4
0.2000
mAU
3 1
150 2
1 5
125
100
0.1000
75
50
25 3

0
0
0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 min 2 4 6 8 10 12 min

Natural Products
App ID 1076

2
Column: Luna 5µ C18(2) 3
Dimensions: 150 x 4.6mm
Order No: 00F-4252-E0
Mobile Phase: A: Acetonitrile/Water (35:65)
B: Acetonitrile/Water (60:40)
Gradient: 100% A in 1min to 100% B in 29 min
Flow Rate: 1.5 mL/min
Detection: UV @ 227nm
1
Sample: 1. Nordihydrocapsaicin
2. Capsaicin
3. Dihydrocapsaicin
4. Homocapsaicin 4

0 10 20 min

Phenomenex 153
 LUNA ®

App ID 14285
Polyamines (Dansylated) Simple Sugars
App ID 1288

Column: Luna 5µ C18 + Security Guard Column: Luna 5µ NH2

Dimensions: 250 x 4.6mm Dimensions: 250 x 4.6mm
Order No: 00G-4252-E0 and KJ0-4282 and AJ0-4287 Order No: 00G-4378-E0
Mobile Phase: A: 93% Methanol in Water Mobile Phase: Acetonitrile / Water (80:20)
B: 65% Acetonitrile in Water Temperature: 40°C
Gradient: A/B (0:100) to A/B (100:0) in 28 min Flow Rate: 3.0 mL/min
to A/B (0:100) in 40 minutes Detection: RI
Temperature: 35°C Sample: 1. Xylose
Flow Rate: 1.5 mL/min 2. Fructose
Detection: 360nm Excitation, 530nm Emission 3. Glucose
Sample: 1. Putrescine 4. Sucrose
2. DHX 5. Maltose
3. DHP 6. Melezitose
4. Spermidine 7. Raffinose
HPLC

5. Spermine
6. Homospermine
7. Methylethylhomospermine
8. Diethylhomospermine (DEHOP)
Luna

App ID 14269

Water-Soluble Vitamins
App ID 2581

PAH’s - EPA Method 8310

Column: Luna 5µ C18(2) Column: Luna 5µ C18(2)
Dimensions: 150 x 4.6mm Dimensions: 250 x 4.6mm
Order No: 00F-4252-E0 Order No: 00G-4252-E0
Mobile Phase: A: Water/methanol, 2g/L hexane sulfonate, Mobile Phase: A: Water
0.2% H3PO4 (95:5) B: Acetonitrile
B: Water/methanol, 2g/L hexane sulfonate, Gradient: A/B (25:75) to A/B (0:100) in 25 min
0.2% H3PO4 (50:50) Temperature: 22°C
Gradient: A/B (80:20) to A/B (27:73) in 17 minutes Flow Rate: 2.0 mL/min
Flow Rate: 1 mL/min Detection: UV @ 254nm
Tempurature: 22°C Sample: 1. Naphthalene 9. Chrysene
Detection: UV-Vis Abs. - Variable Wave. @ 210-280 nm 2. Acenaphthalene 10. Benzo[e]pyrene
Sample: 1. Calcium Pantothenate 3. Fluorene 11. Benzo[b]fluoranthene
2. Niacinamide 4. Phenanthrene 12. Benzo[k]fluoranthene
3. Vitamin B6 5. Anthracene 13. Benzo[a]pyrene
4. Riboflavin 6. Fluoranthracene 14. Dibenzo[a,h]anthracene
5. Thiamine 7. Pyrene 15. Benzo[g,h,i]perylene
8. Benzo[a]anthracene 16. Indenol[1,2,3-c,d]pyrene

154 Phenomenex
 LUNA ®

Fatty Acid Amides Natural Products

App ID 1291

App ID 1138
Column: Luna 5µ C18(2) Column: Luna 5µ Silica(2)
Dimensions: 250 x 4.6mm Dimensions: 150 x 4.6mm
Order No: 00G-4252-E0 Order No: 00F-4274-E0
Mobile Phase: A: 50mM Sodium phosphate pH 6.0 Mobile Phase: Hexane/Dioxane (85:15)
B: Acetonitrile Flow Rate: 1.5 mL/min
Gradient: A/B (75:25) to A/B (35:65) in 15 min, Detection: UV @ 230nm
then 5 min hold at A/B (35:65) Sample: 1. Dihydrokawain
Temperature: 50°C 2. Yangonin 1
Flow Rate: 0.8 mL/min 3. Kawain
Detection: UV @ 214nm 4. Dihydromethysticin
Sample: 1. Acetamide 5. Methysticin
3
2. n-Butyramide
3. Isovaleramide

HPLC
4. n-Hexanamide
4
5. n-Heptamide
2
6. n-Octanamide 5
7. n-Decanamide

Luna
0 10 20 min

Caffeine and Metabolites Polar Herbicides

App ID 1255

App ID 1322

Column: Luna 5µ C18(2) Column: Luna 3µ Phenyl-Hexyl

Dimensions: 150 x 4.6mm Dimensions: 100 x 4.6mm
Order No: 00F-4252-E0 Order No: 00D-4256-E0
Mobile Phase: 50mM Sodium Acetate/Acetonitrile/Methanol(92:4:4) Mobile Phase: A: Acetonitrile B: Water
Flow Rate: 1.0 mL/min Gradient: A/B (40:60) to A/B (40:60) in 3.0 min
Detection: UV @ 273nm to A/B (60:40) in 3.2 minutes
Sample: 1. Theobromine Temperature: 22°C
2. Paraxanthine Flow Rate: 1 mL/min
3. Theophylline Detection: MS @ 195°C
4. Excipient Sample: 1. Simazine
5. Caffeine 2. Methabenzthiazuron
1
3. Chlortoluron
4. Atrazine
2
3 5. Isoproturon
6. Diuron
7. Propazine
8. Linuron
9. Terbutryn
5
10. Trietazine
4

0 5 10 15 20 min
App ID 14296

Tocopherols (Vitamin E) Benzodiazepines

App ID 1243

Column: Luna 5µ NH2 Column: Luna 5µ Phenyl-Hexyl

Dimensions: 250 x 4.6mm Dimensions: 150 x 4.6mm
Order No: 00G-4378-E0 Order No: 00F-4257-E0
Mobile Phase: Hexane/Isopropyl Alcohol (97:3) Mobile Phase: Acetonitrile/Water (80:20)
Flow Rate: 4.0 mL/min Flow Rate: 1.0 mL/min
Detection: UV @ 298nm Detection: UV @ 235nm
Sample: 1. a-Tocopherol Sample: 1. Lormetazepam
2. b-Tocopherol 2. Diazepam
3. g-Tocopherol 3. Oxazepam
4. d-Tocopherol

3
mAU 1 2
50
40
30
20
10
0
0 0.5 1.0 1.5 2.0 2.5 3.0 min

Phenomenex 155
 LUNA ®

App ID 12924
Deoxynucleosides Corticosteroids
App ID 14557

Column: Luna 5µ SCX Column: Luna 5µ CN

Dimensions: 150 x 4.6mm Dimensions: 150 x 4.6mm
Order No: 00F-4398-E0 Order No: 00F-4255-E0
Mobile Phase: 50mM KH2PO4, pH 2.5 Mobile Phase: A: Hexane B: Methylene Chloride/Methanol (80:20)
Flow Rate: 1.5 mL/min 80:20 A/B
Detection: UV @ 210nm Flow Rate: 1.0 mL/min
Injection Volume: 5µL Detection: UV @ 254nm
Sample: 1. d-Uridine 4. d-Cytidine Sample: 1. Desoxycorticosterone
2. d-Inosine 5. d-Adenosine 2. Corticosterone
3. d-Guanosine
3. Cortisone
1
4. Prednisone
5. Prednisolone
HPLC

2 1

4
2 3 5

4
3
Luna

0 2 4 6 8 10 12 14 16 18 min
2 4 6 8 10 12 min

DNPH Aldehydes
App ID 2546
App ID 12923

Pesticides/Herbicides
Column: Luna 5µ C18(2)
Column: Luna 5µ CN Dimensions: 250 x 4.6mm
Dimensions: 150 x 4.6mm Order No: 00G-4252-E0
Order No: 00F-4255-E0 Mobile Phase: A: 99.9% Water, 0.1% THF
Mobile Phase: 18:82 Acetonitrile/Water to 65:35 in 5 min B: 99.9% Acetonitrile, 0.1% THF
Flow Rate: 1.0 mL/min
Gradient: 70:30 (A/B) linear to 30:70 over 30 minutes
Detection: UV @ 254nm
Flow Rate: 1.5mL/min
Sample: 1. Tebuthiuron
Detection: UV @ 380nm
2. Carbofuran
3. Carbaryl Temperature: Ambient
4. Diuron Injection Volume: 25µL
5. Captan 4 Sample: 1. Formaldehyde DNPH (.14µg)
6. Barban 2. Acetaldehyde DNPH
3. Acetone DNPH
4. Acrolein DNPH
3 6 5. Propionaldehyde DNPH
1 2 6. Butyraldehyde DNPH
7. Benzaldehyde DNPH
5

2 4 6 8 min

Carbamates
App ID 1292

Column: Luna 3µ C18(2)

Dimensions: 150 x 2.0mm
Order No: 00F-4251-B0
Mobile Phase: A: Water B: Acetonitrile
Gradient: A/B (90:10) to A/B (30:70) in 20 min
6
to A/B (90:10) in 2 min then 5 min hold at A/B (90:10) 30000 7

Flow Rate: 0.2 mL/min

Detection: API-ES (LC/MS) 25000

Temperature: 42°C
4
Sample: 1. Aldicarb Sulfoxide 20000 3
2. Oxamyl
15000
3. Methomyl 2
5
1
4. 3-Hydroxy Carbofuran
10000 9
5. Aldicarb 8

6. Propoxur
5000
7. Carbofuran
8. Carbaryl 0
9. Methiocarb
5 10 15 20 25 min

156 Phenomenex
 LUNA ®

If Luna does not provide at least an equivalent separation as compared to a

competing column of the same particle size, similar phase and dimensions, send
in your comparative data within 45 days and keep the Luna column for FREE.
Luna Bonded Phase Selectivity Chart
Description Application
C5 5, 10µ
A reversed-phase chemistry that offers greater hydrolytic stability and Highly hydrophobic compounds. Good alternative to C8 or C18 when
alternative selectivity over the classic C4 phase. less retention is desired.
C8(2) 3, 5, 10, 10-PREP, 15µ
C8 phase for excellent efficiency, peak shape and resolution. Significantly Great starting point for methods development of pharmaceuticals,
improved performance over traditional C8 phases due to high surface nucleotides and polar compounds. Excellent for highly aqueous and
coverage. Slightly lower carbon load than original Luna C8. LC/MS applications.
C8 5, 10µ
Original Luna C8 phase. Highly retentive C8 optimized for long lifetimes at A “workhorse” for extremely high pH applications, or when a more
extremely high pH levels. retentive C8 is desired.
C18(2) 3, 5, 10, 10-PREP,15µ
C18 phase. Excellent efficiency, peak shape and resolution. Slightly lower A C18 phase for virtually all HPLC applications. Range of particle sizes

HPLC
carbon load than original Luna C18. offers amazing versatility for capillary and LC/MS, to prep and
process scale applications. Our No.1 recommended choice for HPLC
method development.
C18 5, 10µ
Original Luna C18 phase. Very high retention, optimized for long lifetimes at A “workhorse” for extremely high pH applications, or when longer
extremely high pH levels. retention is desired.
Phenyl-Hexyl 3, 5, 10, 10-PREP,15µ

A (patented) phenyl phase which employs a hexyl alkyl linker as opposed to
the traditional propyl chain. Offers great stability as well as alternative
selectivity.
CN 3, 5, 10µ
Cyano phase. Can be used as reversed- or normal-phase material. The use of
Luna base silica results in overall phase reproducibility and performance.
NH2 3, 5, 10µ
Amino Phase. Can be used in reversed or normal phase modes. Stable from
pH 1.5 to 11.0 and under 100% aqueous conditions. High performance silica
and bonding techniques produces a rugged, highly reproducible column.
SCX 5, 10µ
A Benzene Sulfonic Acid bonded phase is used to make this Strong Cation
Exchange (SCX) column. Offers great peak shape and resolution.
Luna
Remarkable selectivity for aromatic compounds. Offers selectivity
advantages of both a phenyl phase and a C6 or C8 alkyl phase. Stable,
reproducible alternative to traditional phenyl phases. Also very good
for amine and polar compound selectivity.
Excellent for improving the retention of polar compounds. Extremely
rapid equilibration makes it very good for rapid screening and gradient
applications. Exceptional normal phase performance.
Excellent for sugar analysis or anionic compounds by reversed phase
chromatography and for compounds capable of hydrogen bonding
under normal phase conditions.
Excellent for cationic and nitrogen containing analytes such as cough
and cold compounds, codeine and morphine alkaloids, and drugs of
abuse. Also useful for 2-dimension chromatography (SCX-RP-MS-MS)
of small peptides and protein digests.

ORDERING INFORMATION

3µ and 5µ Capillary Columns (mm)

50 x 0.30 150 x 0.30 250 x 0.30 50 x 0.50 150 x 0.50 250 x 0.50 50 x 0.70 150 x 0.70 250 x 0.70
Phases
3µ C8(2) I 00B-4248-AC I 00F-4248-AC I 00G-4248-AC I 00B-4248-AF I 00F-4248-AF I 00G-4248-AF I 00B-4248-AG I 00F-4248-AG I 00G-4248-AG
3µ C18(2) 00B-4251-AC 00F-4251-AC 00G-4251-AC 00B-4251-AF 00F-4251-AF 00G-4251-AF 00B-4251-AG 00F-4251-AG 00G-4251-AG
5µ C8(2) 00B-4249-AC 00F-4249-AC 00G-4249-AC 00B-4249-AF 00F-4249-AF 00G-4249-AF 00B-4249-AG 00F-4249-AG 00G-4249-AG
5µ C18(2) 00B-4252-AC 00F-4252-AC 00G-4252-AC 00B-4252-AF 00F-4252-AF 00G-4252-AF 00B-4252-AG 00F-4252-AG 00G-4252-AG

SecurityGuard™Cartridges require universal holder Order No.: KJO-4282

3µ Microbore and Minibore Columns (mm) SecurityGuard™Cartridges
30 x 1.0 50 x 1.0 150 x 1.0 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0mm 4 x 3.0mm
Phases
Silica(2) I — I — I — I 00A-4162-B0 I 00B-4162-B0 I 00D-4162-B0 I 00F-4162-B0 AJO-4347 I AJO-4348
C8(2) 00A-4248-A0 00B-4248-A0 00F-4248-A0 00A-4248-B0 00B-4248-B0 00D-4248-B0 00F-4248-B0 AJO-4289 AJO-4290
C18(2) 00A-4251-A0 00B-4251-A0 00F-4251-A0 00A-4251-B0 00B-4251-B0 00D-4251-B0 00F-4251-B0 AJO-4286 AJO-4287
CN — — — 00A-4254-B0 00B-4254-B0 00D-4254-B0 00F-4254-B0 AJ0-4304 AJ0-4305
Phenyl-Hexyl 00A-4256-A0 00B-4256-A0 — 00A-4256-B0 00B-4256-B0 00D-4256-B0 00F-4256-B0 AJO-4350 AJO-4351
NH2 — — 00F-4377-A0 00A-4377-B0 00B-4377-B0 00D-4377-B0 00F-4377-B0 AJO-4301 AJO-4302
for ID: 2.0-3.0mm 3.2-8.0mm

3µ Analytical Columns (mm) SecurityGuard™Cartridges

30 x 3.0 50 x 3.0 150 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 4 x 2.0mm 4 x 3.0mm
Phases
Silica(2) I — I — I 00F-4162-Y0 I — I 00B-4162-E0 I — I 00D-4162-E0 I 00F-4162-E0 AJ0-4347 I AJ0-4348
C8(2) 00A-4248-Y0 00B-4248-Y0 00F-4248-Y0 00A-4248-E0 00B-4248-E0 00C-4248-E0 00D-4248-E0 00F-4248-E0 AJ0-4289 AJ0-4290
C18(2) 00A-4251-Y0 00B-4251-Y0 00F-4251-Y0 00A-4251-E0 00B-4251-E0 00C-4251-E0 00D-4251-E0 00F-4251-E0 AJ0-4286 AJ0-4287
CN — 00B-4254-Y0 00F-4254-Y0 00A-4254-E0 00B-4254-E0 00C-4254-E0 00D-4254-E0 00F-4254-E0 AJ0-4304 AJ0-4305
Phenyl-Hexyl — 00B-4256-Y0 00F-4256-Y0 00A-4256-E0 00B-4256-E0 00C-4256-E0 00D-4256-E0 00F-4256-E0 AJ0-4350 AJ0-4351
NH2 — 00B-4377-Y0 00F-4377-Y0 00A-4377-E0 00B-4377-E0 00C-4377-E0 00D-4377-E0 00F-4377-E0 AJO-4301 AJO-4302
for ID: 2.0-3.0mm 3.2-8.0mm
See p. 263 for Fused Silica Capillary Adapter
and Capillary Guard Columns.

Phenomenex 157
 LUNA ®

ORDERING INFORMATION

MercuryMS™ LC/MS Cartridges*

10 x 2.0 10 x 4.0 20 x 2.0 20 x 4.0
Qty Pricing

3µ Phase I I I I
LUNA C18(2) 00N-4251-B0-CE 00N-4251-D0-CE 00M-4251-B0-CE 00M-4251-D0-CE
LUNA C8(2) 00N-4248-B0-CE 00N-4248-D0-CE 00M-4248-B0-CE 00M-4248-D0-CE
5µ
LUNA C18(2) 00N-4252-B0-CE 00N-4252-D0-CE 00M-4252-B0-CE 00M-4252-D0-CE
LUNA C8(2) 00N-4249-B0-CE 00N-4249-D0-CE 00M-4249-B0-CE 00M-4249-D0-CE
HPLC

MercuryMS Cartridges
require holder, see pg. 266

SecurityGuard™Cartridges require universal holder Order No.: KJO-4282

Luna

5µ Microbore and Minibore Columns (mm) SecurityGuard™Cartridges

50 x 1.0 150 x 1.0 250 x 1.0 30 x 2.0 50 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0mm 4 x 3.0mm
Phases
Silica(2) I — I — I — I — I 00B-4274-B0 I 00F-4274-B0 I 00G-4274-B0 AJ0-4347 I AJ0-4348
C5 — — — 00A-4043-B0 00B-4043-B0 00F-4043-B0 — AJ0-4292 AJ0-4293
C8 — — — 00A-4040-B0 00B-4040-B0 00F-4040-B0 00G-4040-B0 AJ0-4289 AJ0-4290
C8 (2) 00B-4249-A0 00F-4249-A0 — 00A-4249-B0 00B-4249-B0 00F-4249-B0 00G-4249-B0 AJ0-4289 AJ0-4290
C18 — — — 00A-4041-B0 00B-4041-B0 00F-4041-B0 00G-4041-B0 AJ0-4286 AJ0-4287
C18 (2) 00B-4252-A0 00F-4252-A0 00G-4252-A0 00A-4252-B0 00B-4252-B0 00F-4252-B0 00G-4252-B0 AJ0-4286 AJ0-4287
CN — — — 00A-4255-B0 00B-4255-B0 00F-4255-B0 00G-4255-B0 AJ0-4304 AJ0-4305
Phenyl-Hexyl 00B-4257-A0 00F-4257-A0 — 00A-4257-B0 00B-4257-B0 00F-4257-B0 00G-4257-B0 AJ0-4350 AJ0-4351
NH2 — 00F-4378-A0 00G-4378-A0 00A-4378-B0 00B-4378-B0 00F-4378-B0 00G-4378-B0 AJ0-4301 AJ0-4302
for ID: 2.0-3.0mm 3.2-8.0mm

5µ Analytical Columns (mm) SecurityGuard™Cartridges

30 x 3.0 50 x 3.0 150 x 3.0 250 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 4 x 2.0mm 4 x 3.0mm
Phases
Silica(2) I — I — I — I — I — I 00B-4274-E0 I — AJ0-4347 I AJ0-4348
C5 — — 00F-4043-Y0 — — 00B-4043-E0 — AJ0-4292 AJ0-4293
C8 — — 00F-4040-Y0 00G-4040-Y0 00A-4040-E0 00B-4040-E0 00C-4040-E0 AJ0-4289 AJ0-4290
C8(2) — 00B-4249-Y0 00F-4249-Y0 00G-4249-Y0 00A-4249-E0 00B-4249-E0 00C-4249-E0 AJ0-4289 AJ0-4290
C18 — — 00F-4041-Y0 00G-4041-Y0 00A-4041-E0 00B-4041-E0 00C-4041-E0 AJ0-4286 AJ0-4287
C18(2) 00A-4252-Y0 00B-4252-Y0 00F-4252-Y0 00G-4252-Y0 00A-4252-E0 00B-4252-E0 00C-4252-E0 AJ0-4286 AJ0-4287
CN — 00B-4255-Y0 00F-4255-Y0 00G-4255-Y0 00A-4255-E0 00B-4255-E0 00C-4255-E0 AJ0-4304 AJ0-4305
Phenyl-Hexyl 00A-4257-Y0 00B-4257-Y0 00F-4257-Y0 00G-4257-Y0 00A-4257-E0 00B-4257-E0 00C-4257-E0 AJ0-4350 AJ0-4351
NH2 — 00B-4378-Y0 00F-4378-Y0 00G-4378-Y0 00A-4378-E0 00B-4378-E0 00C-4378-E0 AJ0-4301 AJ0-4302
SCX — — — — — 00B-4398-E0 — AJ0-4307 AJ0-4308
for ID: 2.0-3.0mm 3.2-8.0mm

158 Phenomenex
 LUNA ®

ORDERING INFORMATION SecurityGuard™Cartridges require universal holder Order No.: KJO-4282

5µ Analytical, Semi-Prep and Preparative Columns (mm) SecurityGuard™ Cartridges

100 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 4 x 2.0mm 4 x 3.0mm 10 x 10mm
Phases
Silica(2) I 00D-4274-E0 I 00F-4274-E0 I 00G-4274-E0 I 00G-4274-N0 I — I 00G-4274-P0 I 00G-4274-U0 AJ0-4347 I AJ0-4348 I AJ0-7223
C5 00D-4043-E0 00F-4043-E0 00G-4043-E0 00G-4043-N0 — — — AJ0-4292 AJ0-4293 AJO-7372
C8 00D-4040-E0 00F-4040-E0 00G-4040-E0 00G-4040-N0 — — — AJ0-4289 AJ0-4290 AJ0-7222
C8(2) 00D-4249-E0 00F-4249-E0 00G-4249-E0 00G-4249-N0 00G-4249-AK 00G-4249-P0 00G-4249-UO AJ0-4289 AJ0-4290 AJ0-7222
C18 00D-4041-E0 00F-4041-E0 00G-4041-E0 00G-4041-N0 — — — AJ0-4286 AJ0-4287 AJ0-7221
C18(2) 00D-4252-E0 00F-4252-E0 00G-4252-E0 00G-4252-N0 00G-4252-AK 00G-4252-P0 00G-4252-U0 AJ0-4286 AJ0-4287 AJ0-7221
CN 00D-4255-E0 00F-4255-E0 00G-4255-E0 00G-4255-N0 — — — AJ0-4304 AJ0-4305 AJ0-7313
Phenyl-Hexyl 00D-4296-E0 00F-4396-E0 00G-4396-E0 00G-4396-N0 00G-4396-AK 00G-4396-P0 00G-4396-U0 AJ0-4350 AJ0-4351 AJ0-7314
NH2 00D-4378-E0 00F-4378-E0 00G-4378-E0 00G-4378-N0 — 00G-4378-P0 00G-4378-U0 AJ0-4301 AJ0-4302 AJ0-7364

HPLC
SCX 00D-4398-E0 00F-4398-E0 00G-4398-E0 00G-4398-N0 — 00G-4398-P0 — AJ0-4307 AJ0-4308 AJ0-7369
for ID: 2.0-3.0mm 3.2-8.0mm 9-16mm

SecurityGuard™
10µ Semi-Prep and Preparative Columns (mm) Guards Cartridges
250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 250 x 50 250 x 100 50 x 50 10 x 10mm

Luna
Phases
Silica(2) I 00G-4091-E0 I 00G-4091-N0 I 00G-4091-AK I 00G-4091-P0 I 00G-4091-U0 I 00G-4091-V0 I 00G-4091-W0 03B-4091-V0 AJO-7223
C5 00G-4092-E0 00G-4092-N0 — 00G-4092-P0 — — — 03B-4092-V0 AJO-7372
C8 00G-4093-E0 00G-4093-N0 — 00G-4093-P0 — 00G-4093-V0 00G-4093-W0 03B-4093-V0 AJO-7222
C8(2) 00G-4250-E0 00G-4250-N0 00G-4250-AK 00G-4250-P0 00G-4250-U0 00G-4250-V0 00G-4250-W0 03B-4250-V0 AJO-7222
C18 00G-4094-E0 00G-4094-N0 — 00G-4094-P0 — 00G-4094-V0 00G-4094-W0 03B-4094-V0 AJO-7221
C18(2) 00G-4253-E0 00G-4253-N0 00G-4253-AK 00G-4253-P0 00G-4253-U0 00G-4253-V0 00G-4253-W0 03B-4253-V0 AJO-7221
CN 00G-4300-E0 00G-4300-N0 — 00G-4300-P0 00G-4300-U0 00G-4300-V0 00G-4300-W0 03B-4300-V0 AJO-7313
Phenyl-Hexyl 00G-4285-E0 00G-4285-N0 00G-4285-AK 00G-4285-P0 00G-4285-U0 00G-4285-V0 00G-4285-W0 03B-4285-V0 AJ0-7314
NH2 00G-4379-E0 00G-4379-N0 — 00G-4379-P0 00G-4379-U0 00G-4379-V0 00G-4379-W0 03B-4379-V0 AJ0-7364
SCX 00G-4401-E0 00G-4401-N0 — 00G-4401-P0 — — — 03B-4401-V0 AJ0-7369
for ID: 9-16mm

15µ Preparative and Pilot Scale Columns (mm) Guards

250 x 4.6 250 x 15 250 x 21.2 250 x 30 250 x 50 250 x 100 50 x 50
Phases
Silica(2) I 00G-4271-E0 I 00G-4271-AK I 00G-4271-P0 I 00G-4271-U0 I 00G-4271-V0 I 00G-4271-W0 03B-4271-V0
C8(2) 00G-4272-E0 00G-4272-AK 00G-4272-P0 00G-4272-U0 00G-4272-V0 00G-4272-W0 03B-4272-V0
C18(2) 00G-4273-E0 00G-4273-AK 00G-4273-P0 00G-4273-U0 00G-4273-V0 00G-4273-W0 03B-4273-V0
Phenyl-Hexyl 00G-4286-E0 00G-4286-AK 00G-4286-P0 00G-4286-U0 00G-4286-V0 00G-4286-W0 03B-4286-V0

See p. 207 for Semi-Prep and Prep

SecurityGuard Cartridge Holders

Phenomenex 159
 LUNA ®

COMBI-HTS™ HIGH-THROUGHPUT
SCREENING COLUMNS
New, 21.2mm and 30mm ID columns provide exceptional loadability and durability
for high-throughput purifications. Luna Combi-HTS columns utilize the same Luna
material, but undergo additional QC at higher flow rates to ensure excellent perfor-
mance under high pressure and high flow conditions.

ORDERING INFORMATION

3µ Columns (mm) 5µ Columns (mm)

HPLC

30 x 2.0 50 x 2.0 30 x 4.6 50 x 4.6 30 x 2.0 50 x 2.0 30 x 4.6 50 x 4.6

Phases Phases
Silica(2) I 00A-4162-B0 I 00B-4162-B0 I — I 00B-4162-E0 Silica(2) I — I 00B-4274-B0 I — I 00B-4274-E0
C8(2) 00A-4248-B0 00B-4248-B0 00A-4248-E0 00B-4248-E0 C5 00A-4043-B0 00B-4043-B0 — 00B-4043-E0
C18(2) 00A-4251-B0 00B-4251-B0 00A-4251-E0 00B-4251-E0 C8 00A-4040-B0 00B-4040-B0 00A-4040-E0 00B-4040-E0
C8 (2) 00A-4249-B0 00B-4249-B0 00A-4249-E0 00B-4249-E0
C18 00A-4041-B0 00B-4041-B0 00A-4041-E0 00B-4041-E0
Luna

C18 (2) 00A-4252-B0 00B-4252-B0 00A-4252-E0 00B-4252-E0

5µ Luna Combi-HTS Columns (mm)

50 x 21.2 100 x 21.2 50 x 30 75 x 30 100 x 30
Phases
Combi-HTS C8(2) I 00B-4384-P0 I 00D-4384-P0 I 00B-4384-U0 I 00C-4384-U0 l 00D-4384-U0
Combi-HTS C18(2) 00B-4383-P0 00D-4383-P0 00B-4383-U0 00C-4383-U0 00D-4383-U0

METHOD
DEVELOPMENT
Luna Method Development Kits combine the excellent perfor-
mance of Luna HPLC columns with either 3 or 5 different se-
lectivities. The column kits include a Luna Phenyl-Hexyl (mod-
erate polarity) and your choice of a Luna CN (polar) or a C8(2)
or C18(2) (non-polars).
METHOD
VALIDATION KITS
Three columns of the same phase from three different batches
provide an excellent benchmark for method validation. Method
reproducibility is easily and quickly assessed, clearly demon-
strating chromatographic performance validation potential and
long term compliance with Luna Method Validation Kits.

ORDERING INFORMATION
Method Development Kits
Order No. Description Dimensions (mm) Unit Price
I I I I
KH0-4760 Luna Method Development Kit (5 columns)
1 each of 5µ C18(2), C8(2), C5, CN, Phenyl-Hexyl phases 150 x 4.6 5/pk
KH0-4354 Luna Rapid-MD Kit (3 columns)
1 each of 3µ C18(2), C8(2) and Phenyl-Hexyl phases 50 x 4.6 3/pk
KHO-4761 Luna Selectivity Kit 1 (3 columns)
1 each of 5µ C18(2), Phenyl-Hexyl, CN phases 150 x 4.6 3/pk
KH0-4762 Luna Selectivity Kit 2 (3 columns)
1 each of 5µ C8(2), Phenyl-Hexyl, CN phases 150 x 4.6 3/pk

Method Validation Kits

Order No. Description Dimensions (mm)* Unit Price
I I I I
KH0-4355 Luna 5µ C18 Method Validation Kit 150 x 4.6 3/pk
KH0-4356 Luna 5µ C18(2) Method Validation Kit 150 x 4.6 3/pk
KH0-4357 Luna 5µ C8 Method Validation Kit 150 x 4.6 3/pk
KH0-4358 Luna 5µ C8(2) Method Validation Kit 150 x 4.6 3/pk
KH0-4359 Luna 5µ Phenyl-Hexyl Method Validation Kit 150 x 4.6 3/pk
*Other dimensions available on request.

See p.264 for MercuryMS LC/MS Cartridge Column System.

See p.268 for LC/MS Computer-Based Training Program.

160 Phenomenex
 LUNA ®

BULK MATERIAL
ORDERING INFORMATION

10µ Bulk Packings

100g 1kg 5kg 10kg 50kg 100kg
Phases
Silica(2) I 04G-4091 I 04K-4091 I 04L-4091 I 04M-4091 I 04N-4091 I 04P-4091
Bonded Silica
C5 04G-4092 04K-4092 04L-4092 04M-4092 04N-4092 04P-4092
C8 04G-4093 04K-4093 04L-4093 04M-4093 04N-4093 04P-4093
C8(2) 04G-4250 04K-4250 04L-4250 04M-4250 04N-4250 04P-4250
C18 04G-4094 04K-4094 04L-4094 04M-4094 04N-4094 04P-4094

HPLC
C18(2) 04G-4253 04K-4253 04L-4253 04M-4253 04N-4253 04P-4253
CN 04G-4300 04K-4300 04L-4300 04M-4300 04N-4300 04P-4300
NH2 04G-4379 04K-4379 04L-4379 04M-4379 04N-4379 04P-4379
SCX 04G-4401 04K-4401 04L-4401 04M-4401 04N-4401 04P-4401
Phenyl-Hexyl
Phenyl-Hexyl 04G-4285 04K-4285 04L-4285 04M-4285 04N-4285 04P-4285

Luna
10µ-PREP Bulk Packings
Lower cost excellent performance alternative to LUNA 10µ.
100g 1kg 5kg 10kg 50kg 100kg
Phases
Silica(2) I 04G-4322 I 04K-4322 I 04L-4322 I 04M-4322 I 04N-4322 I 04P-4322
Bonded Silica
C8(2) 04G-4323 04K-4323 04L-4323 04M-4323 04N-4323 04P-4323
C18(2) 04G-4324 04K-4324 04L-4324 04M-4324 04N-4324 04P-4324
Phenyl-Hexyl
Phenyl-Hexyl 04G-4325 04K-4325 04L-4325 04M-4325 04N-4325 04P-4325

15µ Bulk Packings

100g 1kg 5kg 10kg 50kg 100kg
Phases
Silica(2) I 04G-4271 I 04K-4271 I 04L-4271 I 04M-4271 I 04N-4271 I 04P-4271
Bonded Silica
C8(2) 04G-4272 04K-4272 04L-4272 04M-4272 04N-4272 04P-4272
C18(2) 04G-4273 04K-4273 04L-4273 04M-4273 04N-4273 04P-4273
Phenyl-Hexyl
Phenyl-Hexyl 04G-4286 04K-4286 04L-4286 04M-4286 04N-4286 04P-4286

Phenomenex 161