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Experiment : Cheek cell.

AIM : To prepare stained temporary mount of human cheek cells and to record
observations and dram its labelled diagrams.

MATERIALS REQUIRED
Sterilised toothpick , watch glass, slide, coverslip, needle, forceps, brush dropper,
water, glycerine, methylene-blue stain, microscope, blotting/filter paper,

THEORY
An animal cell is surrounded by a semi-permeable cell membrane around the
cytoplasm. Cell wall is absent in animal cells . Human cheek cells are living and
contain dense and granular cytoplasm The vacuoles are smaller in size or absent in
animal cells. Nucleus lies in the centre of the cytoplasm.

PROCEDURE
1. Rinse the mouth with fresh water and disinfectant solution.
2. Take a clean sterilised toothpick. Gently scrap the inner side of your cheek to
collect some epithelial cells.
3. Take a clean glass slide. Put 1-2 drops of distilled water in the centre of the
slide. Transfer the scrapping to the centre of the slide and spread it with a
needle.
4. Add a drop of methylene blue stain.
5. Wait for 1-2 minutes so that cells become blue stained.
6. Place the coverslip carefully on it by using a needle, so that no air-bubbles
enter the slide.
7. Remove excess stain with blotting paper.
8. Focus the slide under the microscope under 10X magnification.
9. Record the observations.

OBSERVATIONS
1. Large number of flat and irregular-shaped cells are seen with a thin cell
membrane.
2. Each cell has a thin cell (plasma) membrane. Cell wall is absent.
3. A darkly stained nucleus lies in the centre of the cell.
4. Cytoplasm lies inside the cell membrane which is granular in appearance.

Nucleus Prominent darkly stained in the centre of cytoplasm. No Character Observation 1. Do not over stain the material. The cells have nucleus in the centre of the granular cytoplasm. Cytoplasm Granular 7. Clean toothpick should be taken for scrapping of cheek cells. The cytoplasm is surrounded by cell membrane. irregular in shape and without any inter cellular spaces and are squamous epithelial type of animal tissue. . 4. Human cheek cells are flat. Scrape the inside of the cheek gently so that no injury is caused. 3. Excess water should be removed from the slide with a blotting paper 5. 3. RESULT 1. The cells observed under 10X magnification of microscope do not show cell wall and large vacuoles. Inter cellular spaces Absent 4. Arrangement Flat compactly arranged 3. Rinse the mouth with fresh water and disinfectant solution. Place the coverslip carefully to avoid air bubbles. 8. Vacuoles Absent or very small. 5. Use clean slide. 6. 4. S. PRECAUTIONS 1. 2. Shape of cell Irregular shape 2. 2. Cell membrane Prominent 5. There are no inter cellular spaces in the cell. Cell wall Absent 6.

(Draw on the left hand side page of your journal) Human Cheek Cells (Squamous Epithelium) .