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DEARESTHA BRILLIANI

2016
MIKROBIOLOGI NEURO

MYCOBACTERIUM TUBERCULOSIS

Microscope (1000x) à Ziehl-neelsen stain ,


showing pink acid fast bacilli in blue background.

ZN staining sputum from patient with TB à


Showing pink, acid fast bacilli against blue
background of pus cells

KLEBSIELLA PNEUMONIAE
1. Microscope (1000x, gram stain) à Showing characteristic short, plump, gram-negative bacilli,
typical member of Enterobacteriaceae

2. Culture on blood agar à Showing the mucoid lactose-fermenting colonies. The cells are
protected by polysaccharide capsules (which cause slimy appearance of the colonies) against
defense reactions of the host.
DEARESTHA BRILLIANI
2016
MIKROBIOLOGI NEURO

3. Culture on MacConkey agar à lactose-fermenting colonies

STAPHYLOCOCCUS EPIDERMIDIS
1. Microscope (x1000) Gram-positive cocci in cluster

2. Culture of S. epidermidis

(Mannitol Salt Agar) (Blood Agar)


Show growth but no fermentation White colonies
of the colonies

STAPHYLOCOCCUS AUREUS
1. Microscope (x1000) Gram-positive cocci in cluster
DEARESTHA BRILLIANI
2016
MIKROBIOLOGI NEURO

2. Culture of S. aureus

Manitol Salt Agar – showing yellow colonies

3. Methycillin resistance-Staphylococcus aureus

MRSA positive on Mueller Hinton Agar with 4%


NaCl and 6mg/L oxacillin, showing no inhibition

MRSA grow on ORSAB medium (OXOID),


showing intense blue colony on a colorless
background media

STREPTOCOCCUS PNEUMONIAE
1. Microscope (x1000) Gram-positive diplococci with lancefield shape
DEARESTHA BRILLIANI
2016
MIKROBIOLOGI NEURO

2. Culture on blood agar à ∝ −ℎ𝑒𝑚𝑜𝑙𝑦𝑡𝑖𝑐 of S. pneumoniae

3. Optochin (“P”) disk à S. pneumoniae is optochin sensitive

STREPTOCOCCUS PYOGENES
1. Microscope (x1000) Gram-positive cocci in chain

2. Hemolysis pattern of the blood agar à 𝛽 − ℎ𝑒𝑚𝑜𝑙𝑦𝑡𝑖𝑐 of S. pyogens (show complete lysis)

Culture on blood agar, showing complete


lyses (beta) of red blood cells and clearing
of the medium around the colony, due to
production of a diffusible hemolysin
(streptolysin)
DEARESTHA BRILLIANI
2016
MIKROBIOLOGI NEURO

3. Showing 𝛽 − ℎ𝑒𝑚𝑜𝑙𝑦𝑡𝑖𝑐 and susceptible to antibiotic bacitracin

ENTEROCOCCUS
Entreococcus faecalis

1. Gram positive cocci in clusters, short chains,


diplococci and single cocci.
2. Non-hemolytic (gamma-haemolytic)
colonies on sheep blood agar.

Enterococcus faecium

1. Gram positive that can coccus in pairs or


chains.
2. Alpha-hemolytic or non-hemolytic bacterium
in the genus Enterococcus

Causing disease : Neonatal meningitidis


DEARESTHA BRILLIANI
2016
MIKROBIOLOGI NEURO

PSEUDOMONAS AERUGINOSA
1. Microscope (1000x) à Gram-negative bacilli

2. Culture of P. aeruginosa

Culture of P. aeruginosa on nutrient agar produce


greenish pyocyanin pigmen, non-lactose
fermenter. Oxidative positive

ESCHERICHIA COLI
1. Microscope (x1000) Gram-negative coccobacilli

2. Culture of E. coli

(MacConkey agar) (Sheep blood agar)


streak plate technique showing 𝛽 − ℎ𝑒𝑚𝑜𝑙𝑦𝑡𝑖𝑐 non – hemolytic strain

CLOSTRIDIUM TETANI

Microscope (x1000) à Anaerobic raquet-shaped bacillus


DEARESTHA BRILLIANI
2016
MIKROBIOLOGI NEURO

Culture of C. tetani in blood agar à swarming


growth

NEISSERIA MENINGITIDIS
1. Microscope (1000x, gram stain) à Gram-negative diplococcus, nonmotile, shape like a kidney
bean; pili; capsulated (LOS)

2. Culture of Neisseria meningitidis

Colonies Neisseria meningitidis on chocolate


agar medium (heated blood agar),
showing pale gray colonies

CRYPTOCOCCUS NEOFORMANS
1. Large gram-positive yeasts, some with orange halos formed by the polysaccharide capsules.

2. Culture of Cryptococcus neoformans à on CHROM agar.


DEARESTHA BRILLIANI
2016
MIKROBIOLOGI NEURO

3. Cryptococcus neoformans à India Ink, showing yeasts with halos (polysaccharide capsules)

RABIES VIRUS
1. Rabies Virus (postmortem) à showing an oval negri bodies (eosinophilic cytoplasmic inclution) in
a brain cell