IGF-1 PROTOCOL

UNOFFICIAL IGF-1 INSTRUCTIONS

(1) Storage of IGF-1 (prior to mixing)

Lyophilized (dry) IGF-1 is stable at room temperature for 3 weeks; however, it
should be stored below -18 degrees Celsius (in the freezer section).

(2) Weight of IGF-1

1 milligram (mg) IGF-1 = 1000 micrograms (mcg) IGF-1
[Dry weight—before mixing]

(3) What to Mix the IGF-1 with

When reconstituting (adding water), It is important to remember that IGF-1 can
get “stuck” in the grooves of the glass bottle it comes packaged in. While glass appears
smooth to the naked eye, under a microscope, it is a convoluted landscape of grooves and
hidden recesses.
By mixing the lyophilized IGF-1 with an “acid water” (e.g. 10mM HCL-- very
dilute hydrochloric acid or dilute acetic acid), the IGF-1 molecules are efficiently
detached from the glass and solubilized in the mixture. Any online “compounding”
laboratory could mix up a 10mM HCL solution. Likewise, any intro chemistry student
should be able to do the same.
If a reliable source of “acid water” cannot be located, mix your IGF-1 powder
with bacteriostatic water—you’ll lose, at worst, 10% of the IGF-1 solution.

(4) Adding the Acid Water

For purposes of mathematical ease, I suggest mixing the dry 1mg (1000mcg) IGF-
1 with 3mL (or 3cc) of the “acid water” mixture.

(5) Preservation of the IGF-1

Using a 1cc insulin syringe, draw 1cc out of the bottle that contains 3cc of the
acid water/IGF-1 mixture. In a separate 1cc insulin syringe, draw up another 1cc of the
solution. Freeze these two loaded insulin syringes. They will be utilized at a later date.
NOTE: Freezing can safely and effectively preserve IGF-1 (even after it’s been mixed).

(6) The Correct Dilution

To the remaining 1cc of IGF-1 that’s left in the glass bottle, add 2cc of
bacteriostatic water. This will return the total volume back up to 3cc.

(7) The Mathematics

(a) The original concentration of the IGF-1 solution was 1mg (1000mcg) IGF-1 in
3cc of water.
(b) Each 1cc that we removed, then, contained approximately 333mcg per 1cc.
1000mcg / 3cc = 333mcg per 1cc
(c) The 1cc that was left in the bottle, then, also contains 333mcg of IGF-1.
 (d) We added 2cc of bacteriostatic water to the bottle and brought the total volume
back up to 3cc. The difference, now, is that we now have 333mcg in 3cc of liquid
(instead of in 1cc)

(e) To determine how much IGF-1 is in 1cc, you must divide by 3

333mcg / 3cc = 111mcg per 1cc

(f) To determine how much IGF-1 is in .10cc (or 1/10th cc), we do the following:
111mcg / 10 = 11mcg per .10cc (or 10 unit marking on insulin syringe)

(8) Effective Dosages of IGF-1

Dosages in the range of 10-20mcg per day (taken 15-20 minutes after training) are
quite effective for building and repairing muscle tissue. More importantly, these
“moderate” dosages (by some people’s estimation) stimulate muscle growth yet escape
rapid “downregulation” of the all important IGF-1 receptors. Without receptors to
recognize the IGF-1, it doesn’t matter how much you inject.
As dosages climb to over 50mcg per day, receptor downgrade increases
exponentially and, from what I’ve observed among bodybuilders, muscle gains come to a
screeching halt.
Bodybuilders will have the most success with IGF-1 if they follow the protocol I
outlined below. Remember, more isn’t always better!

IGF-1 PROTOCOL DOSAGE TOTALS

11mcg per day for 30 days (Cycle 1) 333mcg

2 weeks OFF 0

11mcg per day for 30 days (Cycle 2)** 333mcg

2 weeks OFF 0

11mcg per day for 30 days (Cycle 3)** 333mcg

8 weeks OFF 0

Total # Weeks: 24 Total IGF-1: 1000mcg (1mg)

**The 2nd and 3rd cycles of IGF-1 treatment require that the two frozen 1cc insulin
syringes be defrosted (only defrost one per cycle). Next, inject the defrosted solution into
an empty bottle. Further dilute with 2cc bacteriostatic water. When adding the 2cc of
water, use the syringe that originally held the frozen IGF-1. This acts to wash the syringe
and ensure that nothing has stuck to the inside of it.