ABSTRACT

ISOLATION OF ACTIVE ANTIOXIDANT COMPOUNDS FROM EXTRACT ETIL

ASETAT LEAF OF SIMPUR PHILIPPINE (Dillenia philippinensis Rolfe)

Nurdiana Khamardi Putri

13161048
Background of the study: The plants of Simpur Philippine (Dillenia philippinensis Rolfe)
include the Dilleniaceae family which has benefits as antimicrobial, antihyperglycemic and
antioxidant. In the previous study it was found that Simpur Philippine contain triterpenoid
compounds, flavonoids and phenols. Leaf ethyl acetate extract has a very strong antioxidant
activity. Purpose of the Study: This study aims to isolate the antioxidant active compound
of ethyl acetate extract of the leaf Simpur Philippine (Dillenia philippinensis Rolfe).
Methods of the Study: Extraction was performed by a multilevel reflux method using n-
hexane solvent, ethyl acetate and methanol. Fractionation and purification using vacuum
liquid chromatography and classical column chromatography. The obtained isolates were
tested for purity using thin layer chromatography with three single and two dimensional
developers. Then, the pure isolate was characterized and identified using UV-Vis
spectrophotometry and IR spectrophotometry. Antioxidant activity test using free radical
damping DPPH method. Result of the Study: DP isolate had antioxidant activity which
reacted to give yellow color with DPPH 0.2% spotting appearance. Identification of DP
isolates with shear reagents has a maximum wavelength of 364 nm and 264 nm which is a
flavonoid compound and allegedly a class of flavonols. From the IR spectral data the DP
isolates contain functional groups of O-H, C = C aromatics, C-H alkyl, C = O, C-H, and C-O.
Conclusion : DP isolate had anticrosidan activity with 32% inhibition at 70 μg / mL
concentration and was thought to be flavonoid flavonoids with OH substitution on atoms C
3.5.6 and 7.

Key Words: Antioxidants, leaf of Simpur Philippine (Dillenia philippinensis Rolfe),

Isolation.