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Unit I
1 Mycology is Study of _ _ _ _ _ _ _ _
A Fungi B Virus
C Prokaryotes D Parasites
A 80s B 65s
C 70s D 75s
A Archaea B Chlamydia
C Mycoplasma D Rickettsia
A Fungi B Rickettsiae
C Algae D Protozoa
7.7 -------------- is mainly present in prokaryotic cells
A Mitochondria B ER
9.9 Which of the following method is not used for total count?
Unit II
2 Concentration range (given as weight of gas per unit chamber volume) for ethylene oxide is
A 15–100mg/L B 800–1200mg/L
C 120–180mg/L D 1000–1200mg/L
A Formaldehyde B Β-propiolactone
A 0.22 mm B 0.45 nm
C 0.22 µm D 30 µm
9 Time in minutes at any defined temperature to destroy 90% of organisms is
A D Value B Z value
C F Value D Q10 value
10 Microorganism used as biological indicator for sterilization by ionizing radiations
A Autoclave B Boiling
A 123℃ B 72℃
C 121℃ D 160℃
17 Bubble point pressure test used to validate _ _ _ _ _ _ method of sterilization
C Filtration D Radiation
C IR waves D Microwaves
20 What is the recommended time and temperature of sterilization in Hot Air Oven
A 160℃, 120 min B 180℃, 120 min
Unit III
1 Phenol coefficient indicates the - - - - - - - - - - - -of a disinfectant
A Quantity B Purity
C Activity D Efficiency
10.2 The choice of antiseptic used for treating gonococci infection in new born is
A Mercurochrome B Silver nitrate
C Ethanol D Formaldehyde
11.4 Time period between each disinfectant dilution with bacterial suspension for sampling Kelsy-Sykes
Test is
A 8 min B 12 min
C 10 min D 18 min
C Herpes D Rabies
13.
7 Chorioallontoic membrane is used for harvesting ------------
C Influenza D Rabies
8 In Rideal-Walker Test, the strain used is
18- In Kelsey-Sykes test, the final concentration of bacterial cells should be about
A 109/ml B 106/ml
C 1010/ml D 108/ml
C Formaldehyde D Glutarldehyde
Unit IV
A acidic B alkaline
Unit V
1 For culturing, plasma from the adult chicken is preferred to mammalian plasma because
A it forms a clear and solid coagulum even B it is too opaque
after dilution
C it doesn't produce solid clots D it forms a semi solid coagulum
2 Disaggregating of cells can be achieved by
A Physical disruption B Enzymatic digestion
C Treating with chelating agents D all of the above
3 Excess CO2 suppress cell growth and productivity by
A inhibiting respiration B altering intracellular pH by diffusing across
cell membrane
C both (a) and (b) D altering pH of the medium
4 The following is the type of microbial spoilage except
A Changes in food colour B Changes in food taste
C Changes in food shape D Changes in food odour
5 In MEM Medium, MEM stands for
A Minimum Effective Medium B Maximum Effective Medium
C Minimum Essential Medium D Maximum Essential Medium
6 Inhibition of microbial growth in tissue culture is by
A Adding surfactants B Adding trypsin
C Adding salts D Adding antibiotics
7 ------------ ml of serum is to be added during the maintenance of cell culture
A 0.3 B 0.4
C 0.1 D 0.5
8 ------------- is the concentration of CO2 required for culturing animal cells
A 2-5% B 2-40%
C 1-10% D 4-5%
9 ---------- is the method of preventing the accumulation of lactate in cell culture
A low glutamine concentration is required B high glutamine concentration is required
C low glucose concentration is required D high glucose concentration is required
10 The following is the main constituents of culture for animal cell growth?
A Glucose and Glutamine B Growth factors
C Cytokines D Antibiotics
11 pH of culture medium is initially controlled by
A Presence of CO2 B Presence of bicarbonate buffer
C Addition of bases D Addition of water
12 In mammalian cell culture, transformation means to see
A uptake of new genetic material B phenotypic modifications of cells in culture
C both (a) and (b) D release of genetic information
13 When cells undergoes transformation frequently then they become
A stable B anchorage dependent
C unstable D anchorage independent
14 Vero cellline is
A Kidney cellline B Lung cellline
C Brain cellline D Skin cellline
15 New born calf serum is used for the purpose of -----------
A Nutrition B pH maintenance
C Viscosity D Inhibition of microbes
16 Serum is a
A Complex media B Simple media
C Enriched medium D Transport medium
17 Higher dissolved oxygen concentration in the culture media are toxic and lead except
A DNA degradation B lipid peroxidation
C Increase growth D Metabolism of nutrients in culture media at a
rate greater than that required for
consumption
18 The following cell culture is having less lifespan
A Primary cell culture B Monolayer of cell
C Secondary cell culture D Established cell culture
19 Cryopreservation is done by
A liquid nitrogen B Preservatives
C Glycerine D Sodium salts
20 Cells that have characteristic features of attachment at the surface is called
A Fixation B Attachment
C Anchorage dependent D Anchorage independent
Dr. K. Mruthunjaya