Name:__________________________________ Class:__________ Date:__________

Chapter : Enzymes
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Multiple-Choice Questions (10 marks)

1. Enzymes are __________.

A Fats
B Proteins
C Carbohydrates
D Inorganic catalysts ( )

2. An enzyme will give a positive result with the __________.

A Benedict’s test
B Starch test
C Ethanol emulsion test
D Biuret test ( )

3. The ‘lock-and-key’ hypothesis explains one of the enzyme characteristics. Which of the
following is the characteristic illustrated by the ‘lock-and-key’ hypothesis?

A Enzymes speed up chemical reactions

B Enzymes are required in minute amounts
C Enzymes are specific in action
D Enzymes are affected by temperature ( )

4. The table below shows 4 different chemical compounds, P, Q, R and S. Which of the
following is an enzyme?

Compound Contains nitrogen Denatured at high

temperature
A P  
B Q  X
C R X  ( )
D S X X

5. The graph below shows the effect of temperature on enzyme reaction.

1
 Rate of
reaction
(enzyme
activity)

Temperature

Which of the following statements best interprets the graph?

A Increase in temperature increases the rate of the enzyme catalysed reaction.

B Increase in temperature decreases the rate of the enzyme catalysed reaction.
C There is a temperature in which the enzyme is most active.
D Extreme temperatures denature the enzyme. ( )

Use the following information to answer questions 6 and 7. The petri dish below is filled
with agar jelly containing starch. Four different solutions are placed into the regions P,
Q, R and S.

P: Amylase and acidic solution

Q: Amylase and alkaline solution
R: Amylase and glucose solution
S: Amylase and distilled water

6. 15 hours later, a few drops of iodine are place on the four different regions. Which of the
regions will give a blue-black colouration?

A P and R only
B Q and S only
C P, R and S only
D Q, R and S only ( )

7. The experiment is repeated with boiled amylase solution. Which of the regions will give
a blue-black colouration?

A P and Q only
B P, R and S only
C Q, R and S only
D All regions ( )

8. The graphs below show the effect of pH on enzyme reaction.

2
 Which of the following statements is correct?

A Each enzyme has its own optimum pH.

B pH 4 is not suitable for enzymes.
C Enzymes works better in alkaline pH.
D Extreme temperatures denature the enzyme. ( )

9. The diagram below shows the process of breaking down of a sucrose molecule into its
products.
X
Y

Which of the following options is correct?

W X Y Z
A Sucrose Sucrase Glucose Fructose
B Sucrase Sucrose Glucose Fructose
C Sucrase Sucrose Glucose Glucose
D Maltase Sucrose Glucose Glucose (
)
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10. Andy noticed that a certain chemical inside apples causes them to turn brown. He wants
to find out if this chemical is an enzyme. How can he determine if the chemical is an
enzyme?

A Peel the apple and see if it turns brown when exposed to air.
B Boil the apple and see if it turns brown when exposed to air.
C Add a few drops of iodine to the apple and see if it turns brown.
D Add a few drops of distilled water to the apple and see if it turns brown. ( )

Structured Questions (15 marks)

11. Mary carried out an investigation to study the effects of temperature and pH on rate of
enzyme activity, using hydrogen peroxide and the enzyme catalase. The enzyme
catalase can be obtained from potatoes.

The enzyme catalase aids in the breakdown of hydrogen peroxide. When broken down,
hydrogen peroxide produces water and oxygen gas.

hydrogen peroxide catalase water + oxygen

The experiment is first carried out by keeping the pH constant and the temperature is
varied. The table below shows the results of Experiment I.

Temperature Time needed to collect 5 cm3 of oxygen

(oC) (minutes)
0 No oxygen produced
10 20
20 15
30 10
40 5
50 17
60 X

The experiment is then repeated with different pH values but keeping the temperature
constant.

The table below shows the results of Experiment II.

pH Time needed to collect 5 cm3 of oxygen

(minutes)
4 24
5 18
6 5
7 18
8 Y

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(a) What is the optimum temperature and pH for this reaction? [2]
Optimum temperature:
Optimum pH:

(b) Suggest a suitable value for Y. [1]

Y: ____

(c) Suggest a suitable value for X. Explain. [3]

X: ____

(d) Why is there a need to keep the pH constant when investigation for the effect of
temperature on enzyme activity? [2]

(e) Mary used only a small amount of potatoes in this experiment. Explain why only
a small amount of potatoes is needed. [2]

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12. Mrs Lim is shopping for laundry detergent that can help remove food stain from her
son’s clothes.

(a) Brand X, a biological washing powder recommended that clothes should be washed at
low temperature, below 50oC. Why?
[3]

(b) Mrs Lim then came across brand Y, also, a biological washing powder, which allows
clothes to be washed at higher temperature. Brand Y uses heat-loving bacteria that can
withstand high temperatures. Brand Y however, is more expensive. Which brand
should Mrs Lim choose?
[2]

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 Question Type of Syllabus Learning Assessment No. of
No. Question Outcome(s) Objectives* Marks
1 3(f) K/C 1
2 3(f) K/C 1
3 3(e) K/C 1
4 3(f) (g) K/C 1
5 3(g) K/C 1
MCQ
6 3(f) K/C 1
7 3(f) A 1
8 3(g) K/C 1
9 3(f) A 1
10 3(f) A 1
11 Structured 3(f), (g) K/C 10
12 Question 3(g) A 5

* K/C: Knowledge/Comprehension
A: Application