Helmy Mohamed Yehia, Ashraf D.

CHE182/A03
Feb. 07,2018 HW #4
A. EMP Pathways/Glycolysis
Steps Reactants Products Enzyme Type of
Reaction
1 Glucose + ATP Glucose-6- Hexokinase Transfer of
Phosphate + ADP Phosphoryl
+ H+ Group
2 Glucose-6-Phosphate Fructose-6- Phosphoglucose Isomerization
Phosphate Isomerace
3 Fructose-6-Phosphate Fructose-1,6- Phosphofructoki Transfer of
+ ATP Bisphosphate + nase Phosphoryl
ADP + H+ Group
4 Fructose-1,6- Dihydroxyacetone Aldolase Split the
Bisphosphate Phosphate molecule in half
(DHAP) +
Glyceraldehyde-3-
Phosphate
5 Dihydroxyacetone Glyceraldehyde-3- Triosphosphate Isomerization
Phosphate Isomerace
6 Glyceraldhyde-3- 1,3- Glyceraldhyde- Oxidation/Phosp
Phosphate + 2NAD+ + Bisphoglycerate + 3-Phosphate hate Ester
2Pi 2NADH + H+ Dehydrogenase Synthesis
7 1,3-Bisphoglycerate + 3 Phosphoglycerat Hydrolysis of
2ADP Phosphoglycerate e Kinase Phosphate
+ 2ATP
8 3 Phosphoglycerate 2 Phosphoglycerat Isomerization
Phosphoglycerate e Mutase
9 2 Phosphoglycerate Phosphoenol Enolase Alcohol
Pyruvate + Water Dehydration
10. Phosphoenol Pyruvate Pyruvate + 2ATP Pyruvate Kinase Phosphate Ester
+ 2ADP + H+ Hydrolysis

Over-all Reaction: Glucose+2ADP + 2NAD+ + 2Pi → 2Pyruvate + 2ATP + 2(NADH + H+)

B.TCA Cycle/Krebs Cycle
Steps Reactants Products Enzyme Type of
Reaction
1 Oxaloacetate + Citrate + CoA-SH Citrate Synthase Condensation
Acetyl-CoA +
Water
2 Citrate + water Isocitrate + water Aconitase Dehydration
then Hydration
3 Isocitrate + NAD+ a-ketoglutarate + Isocitrate Oxidation and
NADH+ H+ + CO2 Dehydrogenase Decarboxylated
4 a-ketoglutarate + Succinyl-CoA + a-ketoglutarate Oxidative
NAD+ + CoA-SH NADH + H+ + CO2 dehydrogenase Decarboxylatio
n
5 Succinyl-CoA + Succinate + CoA-SH Succinyl-CoA Phosphoryl
GDP + Pi + GTP Synthetase Group Transfer
6 Succinate + FAD Fumarate + FADH2 Succinic Oxidation and
Dehydrogenase Hydration
7 Fumarate + Water Malate Fumarase Hydration
8 Malate + NAD+ Oxaloacetate + Malate Oxidation
NADH + H+ Dehydrogenase

Over-all Reaction: Acetyl CoA + 3NAD+ + FAD + GDP + Pi + 2H2O → 2CO2 + 3NADH +
FADH2 + GTP + 2H+ +CoA