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4. Serapan sampel
Replikasi Absorbans
1 0,816
2 0,817
3 0,817
B. PERHITUNGAN
Pembuatan Larutan Standar
Konsentrasi (ppm) larutan parasetamol 10 mg dalam 10 ml adalah :
10 𝑚𝑔
Parasetamol :
10 𝑚𝐿
10 𝑚𝑔
=
0,01 𝐿
10 𝑚𝑔
=
10 𝑚𝐿
= 100 mg/L
= 1000 μg/mL
= 1000 ppm
1000 μg
V1 =
1000 μg/mL
V1 = 1 mL
250 μg
V1 =
100 μg/mL
V1 = 2,5 mL
10 µg/mL x V1 = 1 µg/mL x 10 mL
10 μg
V1 =
10 μg/mL
V1 = 1 mL
10 µg/mL x V1 = 2 µg/mL x 10 mL
20 μg
V1 =
10 μg/mL
V1 = 2 mL
5. Perhitungan larutan standar parasetamol 3 ppm
M1 x V1 = M2 x V2
10 µg/mL x V1 = 3 µg/mL x 10 mL
30 μg
V1 =
10 μg/mL
V1 = 3 mL
10 µg/mL x V1 = 4 µg/mL x 10 mL
40 μg
V1 =
10 μg/mL
V1 = 4 mL
10 µg/mL x V1 = 5 µg/mL x 10 mL
50 μg
V1 =
10 μg/mL
V1 = 5 mL
X Y X2 Y2 XY
(µg/mL) (absorbansi)
1 0,197 1 0,038809 0,197
2 0,382 4 0,145924 0,764
3 0,394 9 0,155236 1,182
4 0,745 16 0,555025 2,98
5 0,747 25 0,558009 3,735
Ʃ = 15 Ʃ = 2,465 Ʃ = 55 Ʃ =1,453003 Ʃ = 8,858
55 . 2,465 − 15 . 8,858
=
5 . 55 − (15)2
135,575 − 132,87
=
275 − 225
2,705
=
50
= 0,0541
𝑛 ∑ 𝑥𝑦 − ∑ 𝑥 ∑ 𝑦
𝑏=
𝑛 ∑ 𝑥 2 − (∑ 𝑥)2
44,29 − 36,975
=
275 − 225
7,315
=
50
= 0,1463
𝑛 ∑ 𝑥𝑦 − ∑ 𝑥 ∑ 𝑦
𝑟2 =
√(𝑛 ∑ 𝑥 2 − (∑ 𝑥)2 )(𝑛 ∑ 𝑦 2 − (∑ 𝑦)2 )
44,29 − 36,975
=
√(275 − 225)(7,265015 − 6,076225)
7,315
=
√(50)(1,18879)
7,315
=
√59,4395
7,315
=
7,70970168
= 0,9488045457
Dari perhitungan diatas didapat persamaan garis linier dari larutan standar paracetamol
= 0,9488045457
0.9
y = 0,0313 + 0,1011x
0.8 r2 = 0.9788482113
absorbansi
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0 1 2 3 4 5 6
Konsentrasi (µg/mL)
(µg/Ml)standar
Kurva kalibrasi (µg/mL)
parasetamol
GANGERTI BUAT YANG INI!!
Perhitungan batas deteksi (LOD) dan batas kuantitasi (LOQ)
X Y yˈ y - yˈ (y - yˈ)2
y = 0,0541 + 0,1463𝑥
1 0,197 0,2004 -0,0034 1,156 x 10-5
2 0,382 0,3467 0,0353 1,24609 x 10-3
3 0,394 0,493 -0,099 9,801 x 10-3
4 0,745 0,6393 0,1057
5 0,747 0,7856 -0,0386
∑(𝑦 − 𝑦′)2
𝑆𝑏 = √
(𝑛 − 2)
4,4651 x 10−3
= √
(5 − 2)
= √0,001488366667
= 0,03857935545
𝑆𝑏
LOD = 3 ×
𝑏
0,03857935545
=3×
0,1011
1,144787996 µg/ml
𝑆𝑏
LOQ = 10 × 𝑏
0,03857935545
= 10 × 0,1011
= 3,815959985 µg/mL