1.1.

Perhitugan Dapar
Larutan dapar fosfat
Diketahui:
pH = 6,5  H+ = 3,162 x 10-7
pKa = 7,21  Ka = 6,165 x 10-8-8
β = 0,01

𝐊𝐚 𝐱 [𝑯+ ]
β = 2,303 . C .
(𝐊𝐚+ [𝑯+ ]) 𝟐

6,165 x 10−8 x 6,165 x10−8

0,01 = 2,303 . C .
[(6,165 x 10−8 )+ (6,165 x10−8 )] 2

19,493 x 10−15
0,01 = 2,303 . C .
(3,778 x 10−7 ) 2

19,493 x 10−15
0,01 = 2,303 . C .
14,273 x 10−14

0,01 = 2,303 . C . 0,136

0,01 = 0,313. C
0,01
C = 0,313

C = 0,031

[𝐠𝐚𝐫𝐚𝐦]
pH = pKa + Log
[𝐚𝐬𝐚𝐦]
[garam]
6,5 = 7,21 + Log
[asam]
[garam]
-0,71 = Log
[asam]
[garam]
= anti Log -0,71
[asam]
[garam]
= 0,194
[asam]

[garam] = 0,194 [asam]

 C = [garam] + [asam]
0,031 = 0,194 [asam] + [asam]
0,031 = 1,194 [asam]
0,031
[asam] = = 0,026 mol/L
1,194

C = [garam] + [asam]
0,031 = [garam] + 0,026
[garam]= 0,031 - 0,026 = 0,005 mol/L

Massa NaH2PO4 = 0,026 mol/L x 120 g/mol

3,12 𝑔
= 3,12 g/L  1000 𝑚𝐿 𝑥 10 𝑚𝐿 = 0,0312 𝑔

Massa Na2HPO4 = 0,005 mol/L x 142 g/mol

0,71 𝑔
= 0,71 g/L  1000 𝑚𝐿 𝑥 10 𝑚𝐿 = 0,0071 𝑔

1.2. Perhitungan Konsentrasi

1g
Cefatoxim = 10 mL x 10 mL = 1 gram
477,45
Cefatoxim Natrium = 𝑥 1 𝑔𝑟𝑎𝑚= 1,05 𝑔𝑟𝑎𝑚
455,47
1,05 𝑔𝑟𝑎𝑚
= 𝑥 100 =10,5%
10 𝑚𝐿

Benzalkonium klorida = 0,01%

0,0312 𝑔
NaH2PO4 = 𝑥 100 = 0,3%
10 𝑚𝐿
0,0071 𝑔
Na2HPO4 = 𝑥 100 = 0,07%
10 𝑚𝐿

Aqua pro Injection ad. 10 mL

1.3. Perhitungan Tonisitas
Konsentrasi Ekivalensi Zat
Nama zat E
(%) (E x %)
Cefatoxim Natrium 0,12 10,5% 1,26
Benzalkonium klorida 0,18 0,01% 0.0018
NaH2PO4 0,03%
 Na2HPO4
Total %
 Sediaan  karena dari 0,9%
 NaCl yang dibutuhkan agar isotonis:
I. Formula Akhir
R/ Cefatoxim Natrium 1,05 g/vial
Benzalkonium Klorida
Na2HPO4
KH2PO4
Aqua Pro Injeksi ad 10 mL