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Stem Cell from Human Exfoliated Deciduous Teeth

(SHED) Transplantation Effect in Neuronal Damage

Reduction in Rat Model of Cerebral Ischemia
Nandini Phalita Laksmi*1, Yetty Ramli1, Salim Harris1, Mohammad Kurniawan1, Masagus Zainuri2,
Ratih Rinendya Putri2, Puspita Eka Wuyung3
1Department of Neurology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
2Center for Research and Development of Biomedical and Basic Health Technology, National Institute Health Research and Development, Ministry of Health Republic of Indonesia,

Jakarta, Indonesia
3Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

Ischemic stroke causes a burden on health problems particularly in Indonesia. Mesenchymal
stem cells (MSCs) are considered as a promising therapy for ischemic stroke at the acute phase.
We evaluated the therapeutic potency of stem cell from human exfoliated deciduous teeth
(SHED) using rat models of ischemic stroke. The aim of this study is to observe the effect of
SHED transplantation on neuronal damage reduction in the infarcted area of brain after
ischemic stroke.
This is a prospective interventional study. Following middle cerebral
artery occlusion (MCAO), fifteen male wistar rats were divided into three
groups : normal rats (n=5), rats undergone permanent MCAO (n=5) as
the control (stroke) group and rats undergone permanent MCAO and
SHED transplantation (n=5) as the treatment group. SHED
transplantation was performed 48 hours after MCAO by intravenous
injection with a dose of 2x10 cells/kg. Evaluation of the neuron death
ratio in the brain cortex area were conducted using Hematoxylin and Figure 1. MCAO Procedure
eosin staining.
Histopathological evaluation with HE staining confirmed that there was a reduction in the
neuron death ratio (p = 0.0) in rats transplanted with SHED. There was no significant differences
at comparative study of neuron death ratio in normal group compare to rats transplanted with

Table 1. The Ratio of Dead Neuron in the Cortex Area

Figure 2. (A) hematoxylin & eosin staining evaluation of neuron in cortex area of Stroke group. The black arrow
marked death neuron, while white arrow marked normal neuron. (B) Hematoxylin & Eosin staining evaluation of
neuron in cortex area of Stroke+SHED group.
SHED transplantation at acute stroke showed reduction in the neuron death ratio in the brain
of rat models with ischemic stroke. SHED transplantation may provide an opportunity for
neuroprotection and neural regeneration after ischemic stroke.
Keyword: Cell transplantation, Ischemic stroke, MCAO, Neuron death ratio, SHED
1. Zhang X, Zhou Y, Li H, Wang R, Yang D, Li B, et al. Transplanted Dental Pulp Stem Cells Migrate to Injured Area and Express Neural Markers
in a Rat Model of Cerebral Ischemia. Cell Physiol Biochem. 2018: 45: 258-266.
2. Inoue T, Sugiyama M, Hattori H, Wakita H, Wakabayashi T, Ueda M. Stem Cells from Human Exfoliated Deciduous Tooth-Derived
Conditioned Medium Enhance Recovery of Focal Cerebral Ischemia in Rats. Tissue Eng Part A. 2013;19:1–2.