Académique Documents
Professionnel Documents
Culture Documents
Dr. SINDHU K.
M. V. Sc. SCHOLAR,
DEPT. OF VPT,
COVAS,
POOKODE.
Extraction
• De Materia Medica,
• Historia Plantarum,
• Species Plantarum.
have been variously published in attempt
to provide scientific information on the
medicinal uses of plants.
Standardized Extraction
The purpose of standardized extraction
procedures for crude drugs
(medicinal & aromatic plant parts)
low toxicity,
ease of evaporation at low heat,
promotion of rapid physiologic absorption of
the extract,
preservative action,
inability to cause the extract to complex or
dissociate.
Solvents used for active component extraction
Water
• Water is universal solvent.
• used to extract plant products with antimicrobial
activity.
• Traditional healers use primarily water &
consistent antimicrobial activity is obtained.
Plant extracts: organic solvents >>> water extract.
• Water soluble flavonoids (mostly anthocyanins)
have no antimicrobial significance.
• only water soluble phenolics are important as
antioxidant compound.
Acetone
• Dissolves many hydrophilic and lipophilic
components.
• a very useful extractant, especially for
antimicrobial studies (phenolic group extract).
• extraction of tannins + phenolics:
aqueous acetone >>> aqueous methanol
• Both acetone and methanol were found to extract
saponins antimicrobial activity.
Alcohol
• The identified components from plants
(antimicrobial) = aromatic or saturated organic
compounds most often obtained through initial
ethanol or methanol extraction.
• Ethanol, found easier to penetrate the cellular
membrane to extract the intracellular
ingredients(polyphenols) from the plant material.
• Methanol is more polar than ethanol but due to its
cytotoxic nature.
• The higher concentrations of more bioactive
flavonoid compounds were detected with ethanol
70% due to its higher polarity than pure ethanol.
Chloroform
• Used to obtain tannins and terpenoids.
1. Size reduction
2. Extraction
3. Filtration
4. Concentration
5. Drying
1. Size Reduction
Objective:
• To rupture plant organ, tissue & cell structures so that its
medicinal ingredients are exposed to the extraction
solvent.
• Size reduction maximizes the surface area, which in turn
enhances the mass transfer of active principle from plant
material to the solvent.
The 30-40 mesh size is optimal.
Hammer mill or a disc pulverizer which has built in sieves
controlled by varying the speed of the rotor clearance
b/w the hammers & the lining of the grinder.
Extraction
• essential oils,
• concretes,
• absolutes,
• pomades
• resinoids.
Essential oils
• Used in a wide variety of consumer goods viz.,
detergents, soaps, toilet products, cosmetics,
pharmaceuticals, perfumes, confectionery food
products, soft drinks, distilled alcoholic
beverages (hard drinks) and insecticides.
• Production technology is an essential element
to improve the overall yield & quality of
essential oil.
Presence of Essential Oils in plant parts.
Hetreogenous chemical group present in essential oils
The traditional methods for essential oil extraction
• Water distillation,
• Water and steam distillation,
• Steam distillation,
• Cohobation,
• Maceration,
• Enfleurage.
methods
Google images.