Vous êtes sur la page 1sur 18

analySIS®

The ADDA Module


About this document
This document describes the software that is used to operate the
ADDA interface with the analySIS® software of Soft Imaging System.
It is assumed throughout the documentation, that the hardware has
been installed correctly and that the necessary parameters to adjust
the ADDA to your microscope have been set correctly. Installation
guidance and technical data can be found in the Installation Manual.
The ADDA software is an extension to the image processing software
analySIS®. For questions relating to the analySIS® software, please
consult the analySIS® User’s Guide.
The ADDA Module

What is the ADDA? 3

Two modes of operation................................................. 3


Combination with EDX systems ..................................... 3
Hardware and cabling .................................................... 4

Software control of the ADDA 4


$''$

Configure input 6

Active ADDA operation................................................... 7


Passive ADDA operation.............................................. 10
EDX operation .............................................................. 13

Acquire images 14

Button bar for special EDX commands 16

EDX Intensity ....................................................................... 16


EDX Linescan ...................................................................... 16
EDX Polygon Scan............................................................... 17
ADDA0799
The ADDA Module

What is the ADDA?


Definition The acronym ADDA stands for analog-digital/digital-analog conver-
ter. This describes the interface between a technical device that
works in analog mode and a digital computer. On the one hand, the
ADDA converts the analog signals of the device into digital pulses that
can be recognized and understood by the computer, on the other
hand it translates the digital signals of the computer into analog
signals that can be used to externally control the devices.
The ADDA interface of Soft Imaging System is used mostly to acquire
digital images from a Scanning Electron Microscope (SEM), instead
of using Polaroid instant film. This allows the user to record the image
of the electron microscopy directly into an image processing
application running on the same computer. In addition the electron
microscope can be used in a very flexible way if the system includes
the active operation mode.

Two modes of operation


Active The active mode of the ADDA interface enables the software to
control the movement of the electron beam within the electron
microscope. Two D/A converters translate the digital pulses of the
computer into analog signals and take control of the beam position.
As in normal SEM mode, an area of the sample is scanned line by
line. The intensity values of the detector are recorded pixel by pixel,
transformed into digital pulses through an A/D converter, and trans-
ferred to the computer, where they are stored. The scanning of the
electron beam thus creates a two-dimensional, digital representation
of the scanned area, which can be displayed, processed and
analyzed on the image-processing computer. Since the active mode
transfers the control of the beam to the computer, any resolution can
be used for image acquisition. This mode is also required for inter-
facing to EDX systems.
Passive In contrast to the active mode, the passive mode of the ADDA does
not take over the beam control from the microscope during scanning.
The ADDA has to be synchronized with the scanning of the micro- See also
scope. Timings 11

Combination with EDX systems


The simplest setup for an electron microscope involves only a single
signal that the ADDA needs to measure. For example, the number of
secondary electrons released from the sample while being bombar-
ded with the primary electrons is such a signal.
The ADDA interface can also acquire several signals simultaneously.
For example the ADDA can be used to acquire EDX counts from
several channels at the same time. Each channel may be assigned to
a specific energy range in the EDX spectrum, which in turn may be
related to the amount of a given element in the sample. These chan-
nels are then acquired simultaneously and the result shows how
many X-rays for a given element were collected during the time the

3
The ADDA Module

beam was stationary. The result is then directly related to the


concentration of the element in the sample.
Further down in this document you can find instructions on how to set See also
up the ADDA if you want to operate it together with an existing EDX EDX operation 13
system.

Hardware and cabling


The ADDA interface from Soft Imaging System consists of a box with
the electronic hardware, cables and the appropriate software. The
ADDA software is a module for the image processing software
analySIS®.
The ADDA box is usually located close to the microscope and
connected to the frame grabber of the computer through two fiber
optic cables. Locating the ADDA close to the microscope has the
advantage of minimizing the length of electrical cables necessary to
transmit the signals from and to the microscope. This is important to
minimize possible electro-magnetic disturbances.
Using fiber optical cables to connect the ADDA box with the frame
grabber of the computer has the advantage, that the signals, once
optically encoded, are immune to electro-magnetic disturbances, and
can be transmitted over long distances without losing information. A
second advantage is that a clear electrical separation of frame
grabber and microscope provides some protection against 50 or 60
Hz noise due to electrical ground loops. This noise is normally one of
the major artifacts on images acquired with SEMs.

Software control of the ADDA


Logical input The ADDA software uses the concept of logical input channels, in line
channels with the standards for analySIS®. This means, that for the user there
are no immediate changes to the analySIS® software when the ADDA
is connected to the microscope and the PC and the ADDA software is
installed. There are no new menus to learn, no new button bars to
keep track of. The changes appear, when the command Set Input...
in the menu Image is used. In addition to the channels available
before, the software now provides additional channels for the ADDA,
which can be configured by the users for their needs. In general, one
or two of these channels will be pre-configured. They contain, for
example, information about the type of signal, the magnification, and
the way the image must be pre-processed before being transmitted to
the analySIS® frame buffer. Each channel must be calibrated sepa-
rately. A typical name for the acquisition channel of the ADDA would
be, e.g., "ADDA" or "GrabBit ADDA". A more detailed explanation of See also
the concept of logical input channels is available in the analySIS® Set Input, Menu Image,
User’s Guide. You will find it with the description of the command Set User’s Guide
Input... in the Image section.
EDX button bar An exception to the rule is the use of the ADDA for interfacing to an See also
EDX system. In this case, an extra button bar becomes available with Button bar for special
3 buttons for EDX measurements. EDX commands 16

4
The ADDA Module

Turn on the In order to use the ADDA box from within analySIS®, it has to be
ADDA box turned on BEFORE analySIS® is started. If that is not the case, the
following error message will be displayed:

Press the button Disable if you wish to use analySIS® without the
ADDA functionality. You will not be able to acquire images or signals
with the ADDA.
Press the button Retry after turning on the ADDA with the power
switch on its front panel if you wish to use the ADDA. A yellow
indicator lamp with the label "Init" will turn on when the communication
between the ADDA and the PC has been established. This lamp will
stay lit as long as the ADDA and the PC communicate.
How to... Select the ADDA interface for the acquisition channel and configure
the appropriate logical channel.
1) Open the menu Image.
2) Select the command Set Input....
→ The dialog Set Input appears:

3) From the list of available input channels select the one you wish to

5
The ADDA Module

configure, for example, "ADDA, GrabBit ADDA".


→ Select the channel by left-clicking on it with the mouse.
→ Depending on your configuration you may see different channels
than shown above.
4) Press the button Configure....
→ The dialog Configure Input appears with 8 separate tabs.
→ To open this dialog directly at a later time, you can select the
command Configure Input... directly from the Image menu.
5) Change the entries on the various tabs to fit your needs.
→ Seven of the tabs look exactly like they do for other input
devices.You can find a thorough explanation of the individual tabs
in the analySIS® User’s Guide with the description of the See also
command Configure Input... in the Image menu. Configure Input, Menu
Image, User’s Guide;
→ The tab Input contains the ADDA-specific entries. This tab will be Configure input 6
described in more detail further down for the three operating
modes of the ADDA: passive, active, and EDX operation.
6) Once all the parameters are set, press the button OK.
→ You are back at the Set Input dialog.
7) Press the button Close.
→ The selected input channel, now "ADDA", is displayed in the
status bar right of center. It is now the active input channel, i.e.,
the next image acquisition proceeds using this channel with the
parameters defined for it.

Configure input
Tab Input The dialog Configure Input includes 8 tabs that are used to See also
determine the parameters for each input channel individually. The How to..., Software
following explains the parameters and options of the tab Input. This control of the ADDA 5
tab is used to control the ADDA specific parameters. An explanation
for the other tabs can be found in the analySIS® User’s Guide with the See also
description of the command Configure Input... in the Image menu. Configure Input, Menu
Image, User’s Guide
3 Cases The layout and the available parameters of the tab Input depend on
the selected input channel. We will consider first the case, that you
have selected the active mode of the ADDA to acquire images from
the microscope. Following that we will describe the passive mode of
image acquisition, and finally we will proceed to the case that the
ADDA is being used to control and read an external EDX system.

6
The ADDA Module

Active ADDA operation

Input In the field Input you select, which of the available 16 analog input
channels is used to acquire an image. Normally this will be input 1.
Image size The parameter group Image size allows the user to define the image
size (in pixels) to be used for acquisition and transfer to the computer.
Note: This entry does not change or influence the area scanned by
the electron beam. It simply determines how many steps the electron
beam takes to traverse a given area.
Width Enter the width and the height (in pixels) of the image you wish to
Height acquire. For example, if you enter 768 under Width and 576 under
Height, the ADDA will acquire an image that is 768 pixels wide and
576 pixels high. The maximum value for both dimensions is 4096.
Keep X/Y ratio You can select the box Keep X/Y ratio to fix the x/y ratio for your
images. If this box is selected and you change one of the height or
width parameters at a later time, the other parameter will be changed
automatically to preserve the x/y ratio. It is recommended to use a
fixed x/y ratio.

7
The ADDA Module

8 bit mode Select the check box 8 bit mode to save the images with 8-bit rather
than 12-bit information. The ADDA interface normally uses 12 bits per
pixel for the intensity values during image acquisition. In the 8-bit
mode the lower four bits are discarded. If you select the check box
Activate on the tab Display in the dialogue Configure Input, menu See also
Image, in the group Automatic gain display, the resulting 8-bit Tab Display, Configure
image on the screen will be optimized as to the distribution of the gray Input, Menu Image,
values. User’s Guide

Application The 8 bit mode is indicated, if you want to use your images later in
another software program that can only use 8 bit images. The speed
of the image acquisition is not affected when you selected this check
box.
If you want to change the bit depth of the images at a later point you
can select the command Change Bit Depth, To 8-Bit in the menu
Oper within the analySIS® program.
Timings The group Timings determines the overall speed of the image acqui-
sition. It includes controls that are used to determine parameters that
have an influence on the speed with which images are acquired. As
a general rule: the typical time for reading of a single pixel is ten
microseconds, a complete line takes about ten milliseconds.
Pixel time Enter into the field Pixel time the dwell time of the electron beam, that
is, the time that the electron beam is fixed on a certain point of the
sample. The larger this dwell time, the less noise will be visible in the
image. A typical value for the dwell time is ten microseconds, the
maximum possible value is 1000 microseconds. Further down you See also
will find a section called "Acquire images". Inside this section you will Camera control 15
find instructions how you can change the dwell time of the beam
interactively, until all you acquire an image that has sufficient quality.
This is only possible when the microscope is controlled externally
through the ADDA interface, that is, when the ADDA interface is
operated in the active mode.
Line time Enter into the field Line time the amount of time that has to pass for
the electron beam to move from the beginning of one line to the
beginning of the next line. A typical value for this number is ten milli-
seconds. The exact value is the product of the Pixel time and the
Width of the image, plus the time needed for the beam to move back
to the beginning of the line. If you change one of the values Pixel
time or Line time, the other value is changed appropriately.
Sensitivity The group Sensitivity contains controls, which determine how
sensitive the ADDA reacts to input voltages.
Before making use of these controls you first should try to optimize the
parameters on your microscope. Only if this is not possible - eg. when
acquiring very low signal amplitudes - values smaller than 100%
should be entered here.
Upper limit Enter into the field Upper limit a value, which must be smaller than
100%, if you wish the maximum pixel intensity (white) at an input
voltage lower than the maximum voltage.

8
The ADDA Module

Lower limit Enter into the field Lower limit a value, which must be smaller than
100%, if you wish the minimum pixel intensity (black) at an input
voltage higher than the minimum input voltage.
Averaging The group Averaging contains a dialog that allows you to perform
averaging of several consecutive images. Using this averaging, an
image with less noise can be acquired.
Snapshot In the field Snapshot you can determine, how many images must be
averaged: 2,4,8,16,32,64,128 or 256. The entry "None" disables the
averaging mode.
16 Bit integration Select the check box 16 Bit integration, if you do not want to average
the images, but to integrate them. In this case the images are added
without being divided by the number of the acquired images after-
wards. The dynamic range of the resulting image can be expanded
considerably using this technique.
Active scan The group Active scan gives access to two settings that are impor-
tant for the active operation mode: You can synchronize the scanning
with the power supply, and you can determine whether the micro-
scope needs a signal to switch into an external control mode.
Warning Note: The settings in this group should be determined during the
setup of the ADDA and should not require changes afterwards.

Mains syn- Select the check box Mains synchronization if you wish to reduce
chronization the detrimental effects of 50 or 60 Hertz noise. In the active mode of
operation the ADDA electronic will move the electron beam to the next
line always at the same phase of the 50 or 60 Hertz power signal.
Although this technique can be used to reduce the 50 or 60 Hertz
noise, it is usually more appropriate to change the cabling and to
avoid ground loops.
No TTL logic Select the option No TTL logic if your microscope does not require a
TTL signal to switch into external mode. TTL is the abbreviation for
transistor-transistor-logic.
Positive Select the option Positive TTL logic when the control signal must be
TTL logic raised from 0V to 5V during the image acquisition.
Negative Select the option Negative TTL logic when the control signal must be
TTL logic lowered from 5V to 0V during image acquisition.
Synchronization In active operation mode the group Synchronization offers only one See also
check box. You will find the description of the other two check boxes Passive ADDA operation 10
in the section "Passive ADDA operation".
Passive scan Select the check box Passive scan if you wish to temporarily disable
the active mode, and you wish to use the microscope scan generator.
If you want to configure a logical input channel for passive mode this
is the check box to be selected first.
Camera The field Camera at the lower edge of the dialog box shows the
currently selected camera. As shown in the screenshot, the entry
"GrabBit ADDA" indicates, that an image is acquired through the
ADDA interface and the GrabBit frame grabber card.

9
The ADDA Module

Passive ADDA operation


If you wish to configure a logical input channel for passive ADDA
operation mode you first have to select the check box Passive scan
in the group Synchronization on the Input tab. Then you determine
the other parameters for that channel.

Input In the field Input you select, which of the available 16 analog input
channels is used to acquire an image. Normally this will be input 1.
Image size The parameter group Image size allows the user to define the image
size (in pixels) to be used for acquisition and transfer to the computer.
Note: This entry does not change or influence the area scanned by
the electron beam. It simply determines how many steps the electron
beam takes to traverse a given area.
Width Enter the width and the height (in pixels) of the image you wish to
Height acquire. For example, if you enter 768 under Width and 576 under
Height, the ADDA will acquire an image that is 768 pixels wide and
576 pixels high. The maximum value for both dimensions is 4096.
Keep X/Y ratio You can select the box Keep X/Y ratio to fix the x/y ratio for your
images. If this box is selected and you change one of the height or
width parameters at a later time, the other parameter will be changed
automatically to preserve the x/y ratio. It is recommended to use a
fixed x/y ratio.

10
The ADDA Module

8 bit mode Select the check box 8 bit mode to save the images with 8-bit rather
than 12-bit information. The ADDA interface normally uses 12 bits per
pixel for the intensity values during image acquisition. In the 8-bit
mode the lower four bits are discarded. If you select the check box
Activate on the tab Display in the dialogue Configure Input, menu See also
Image, in the group Automatic gain display, the resulting 8-bit Tab Display, Configure
image on the screen will be optimized as to the distribution of the gray Input, Menu Image,
values. User’s Guide

Application The 8 bit mode is indicated, if you want to use your images later in
another software program that can only use 8 bit images. The speed
of the image acquisition is not affected when you selected this check
box.
If you want to change the bit depth of the images at a later point you
can select the command Change Bit Depth, To 8-Bit in the menu
Oper within the analySIS® program.
Timings The group Timings determines the overall speed of the image acqui-
sition. The numbers entered into the fields Pixel time and Line time
must be synchronized with the scan generator of the microscope, so
that a complete image can be acquired. As a general rule: the typical
time for reading of a single pixel is ten microseconds, a complete line
takes about ten milliseconds.
Warning In the passive operation mode image size and timings must be syn-
chronized with the microscope scan generator. If this is not the case
the image will show artifacts like distortions, etc.
Pixel time Enter into the field Pixel time the dwell time of the electron beam, that
is, the time that the electron beam is fixed on a certain point of the
sample.
Line time Enter into the field Line time the amount of time that has to pass for
the electron beam to move from the beginning of one line to the be-
ginning of the next line. The value is the product of the Pixel time and
the Width of the image. If you change one of the values Pixel time or
Line time, the other value is changed appropriately.
Sensitivity The group Sensitivity contains controls, which determine how
sensitive the ADDA reacts to input voltages.
Upper limit Enter into the field Upper limit a value, which must be smaller than
100%, if you wish the maximum pixel intensity (white) at an input
voltage lower than the maximum voltage.
Lower limit Enter into the field Lower limit a value, which must be smaller than
100%, if you wish the minimum pixel intensity (black) at an input
voltage higher than the minimum input voltage.
Averaging The group Averaging contains a dialog that allows you to perform
averaging of several consecutive images. Using this averaging, an
image with less noise can be acquired.
Snapshot In the field Snapshot you can determine, how many images must be
averaged: 2,4,8,16,32,64,128 or 256. The entry "None" disables the
averaging mode.

11
The ADDA Module

16 Bit integration Select the check box 16 Bit integration, if you do not want to average
the images, but to integrate them. In this case the images are added
without being divided by the number of the acquired images after-
wards. The dynamic range of the resulting image can be expanded
considerably using this technique.
Active scan In passive operation mode the group Active scan is not available.
Synchronization In the group Synchronization you turn on the passive operation
mode, and furthermore you find two check boxes that are important
for this mode: You can determine, if the microscope generates signals
with negative logic (i.e., changes from 5V to 0V) instead of positive
logic (i.e., changes from 0V to 5V).
Passive scan For the passive operation mode the check box Passive scan has to
be selected. The ADDA interface then uses the microscope scan
generator for image acquisition. It is best to select this check box first
before determining the other parameters on all tabs.
Invert line Select the check box Invert line blank if your microscope uses
blank negative logic for the voltage signals that cause a line retrace.
Invert frame Select the check box Invert frame blank if your microscope uses
blank negative logic for the voltage signals that cause a frame retrace.
Warning Note: The settings for line blank and frame blank should be
determined during the setup of the ADDA and should not require
changes afterwards.
Camera The field Camera at the lower edge of the dialog box shows the
currently selected camera. As shown in the screenshot, the entry
"GrabBit ADDA" indicates, that an image is acquired through the
ADDA interface and the GrabBit frame grabber card.

12
The ADDA Module

EDX operation

Active input Use the controls in the group Active input channels to select, which
channels of the 4 channels you wish to use for EDX acquisition.
Image size The parameter group Image size allows the user to define the image
size (in pixels) to be used for EDX acquisition and transfer to the
computer. Note: This entry does not change or influence the area
scanned by the electron beam. It simply determines how many steps
the electron beam takes to traverse a given area.
Width Enter the width and the height (in pixels) of the images you wish to
Height acquire. For example, if you enter 768 under Width and 576 under
Height, for each selected EDX channel the ADDA will acquire an
image that is 768 pixels wide and 576 pixels high. The maximum
value for both dimensions is 4096.
Keep X/Y ratio You can select the box Keep X/Y ratio to fix the x/y ratio for your
images. If this box is selected and you change one of the height or
width parameters at a later time, the other parameter will be changed
automatically to preserve the x/y ratio. It is recommended to use a
fixed x/y ratio.
Timings The group Timings determines the overall speed of the image acqui-
sition. It includes controls that are used to determine parameters that
have an influence on the speed with which EDX data are acquired. As
a general rule: the typical time for reading of a single pixel is ten
milliseconds, a complete line takes about ten seconds.

13
The ADDA Module

Pixel time Enter into the field Pixel time the dwell time of the electron beam, that
is, the time that the electron beam is fixed on a certain point of the
sample. The larger this dwell time, the less noise will be visible in the
image. A typical value for the dwell time for EDX acqusition is ten
milliseconds.
Line time Enter into the field Line time the amount of time that has to pass for
the electron beam to move from the beginning of one line to the
beginning of the next line. A typical value for this number for EDX
acquisition is ten seconds. The exact value is the product of the Pixel
time and the Width of the image. If you change one of the values
Pixel time or Line time, the other value is changed appropriately.
TTL control In the group TTL control (transistor-transistor-logic) you determine
whether the microscope needs a signal to switch into an external
control mode.
Warning Note: The settings in this group should be determined during the
setup of the ADDA and should not require changes afterwards.

None Select the option None if your microscope does not require a TTL
signal to switch into external mode.
Positive logic Select the option Positive logic when the control signal must be
raised from 0V to 5V during the image acquisition.
Negative logic Select the option Negative logic when the control signal must be
lowered from 5V to 0V during image acquisition.
Acquire as 8 bit Select the check box Acquire as 8 bit image to save the EDX images
image with 8-bit rather than 16-bit information. The ADDA interface normally
uses 16 bits per pixel for the intensity values during EDX image
acquisition. By converting them to 8 bits considerably less memory is
used. In addition, the images are normally better visible for weak EDX
signals, and you can use the images with software that is unable to
use 16-bit gray value images.
Camera The field Camera at the lower edge of the dialog box shows the
currently selected camera. As shown in the screenshot, the entry
"GrabBit EDX" indicates, that an EDX image is acquired through the
ADDA interface and the GrabBit frame grabber card.

Acquire images
Yellow, green When the ADDA box is turned on, analySIS® is loaded on your See also
light computer, and the communication of the computer with the ADDA is Turn on the ADDA box 5
working normally, a yellow light "Init" will be lit on the front face of the
ADDA box. This indicates that the system is ready for image
acquisition. When you start the acquisition, a green light "Scan" will
turn on. It will turn off again, when the image acquisition is finished
and the data have been transferred to the computer.

14
The ADDA Module

Set Input To select the desired input channel, select the command Set Input...
from the menu Image. For a description of the use of this command, See also
please read the chapter "Software control of the ADDA". Select the How to..., Software
channel you want to use for the acquisition, either the normal ADDA control of the ADDA 5
microscope acquisition (passive or active) or the EDX input channel.
Snapshot As with any other image acquisition hardware in analySIS®, select the See also
Acquire command Snapshot in the menu Image if you wish to acquire a Acquire/Snapshot, Menu
single image. For the display of a continuously updated live image Image, User’s Guide
select the command Acquire from the menu Image. During live
acquisition, the camera symbol in the status bar flashes. Selecting the
command Snapshot terminates the live acquisition mode.
EDX images Selecting the command Snapshot with EDX acquisition causes the
system to read and acquire multiple channels in parallel. They will be
stored in consecutive frame buffers. How many channels are acquired
simultaneously depends on your choice in the group Active input See also
channels on the Input tab of the appropriate input channel. The com- Active input channels 13
mand Acquire is not available for EDX image acquisition, because
the acquisition of EDX images requires too much time.
Camera Control In active ADDA operation mode selecting the command Camera
Control... in the menu Image opens a dialog box, where you can
manipulate the Pixel time and thus the image quality interactively
while observing the live image on the screen:

In passive ADDA operation mode this command is also available, but


you cannot manipulate the Pixel time interactively while acquiring a
live image.
The same is true for EDX operation mode because live images are
not possible at all in this mode.
- + Pixel time Press the buttons "-" or "+" or enter a value directly in the field Pixel
time to change the dwell time of the beam on each pixel. Continue this
until you are satisfied with the image quality. The use of the "-" or "+"
buttons changes the Pixel time in pseudologarithmic steps: 1, 2, 5, 10,
20, 50, 100, 200, ... All values that depend on this value, such as Line
time and Frame time are updated automatically. This applies also to See also
the values in the group Timings on the tab Input. Timings 8, 11, 13

15
The ADDA Module

Button bar for special EDX commands


For EDX measurements a separate button bar is available in addition
to the commands already mentioned above. The button bar contains
three additional buttons:

EDX Intensity
EDX Linescan
EDX Polygon Scan

These commands allow you to measure EDX signals originating from


selected locations on your sample - without acquiring a whole EDX
image.

EDX Intensity
Press the button EDX Intensity to open the dialog EDX Intensity:

The bar diagram displays how many EDX counts are collected within
the pixel time through the selected EDX channels. Use the micro- See also
scope to move the beam to the desired location on the sample. Pixel time 14

EDX Linescan
Press the button EDX Linescan to record an intensity profile along a
straight line. When using this command you have to record an image
through the ADDA interface before. This image has to be stored in the
active image buffer.
The mouse pointer is transferred to the image in the active image
buffer. Now draw a line into the image by clicking on the starting point
with the left mouse button and then the ending point with another click
of the left mouse button. The Linescan dialog box will be opened:

16
The ADDA Module

Number of points Enter the Number of points you wish to use along the line scan. After
you press OK, the EDX counts will be measured. analySIS® will open
a table and enter the number of EDX counts for each point along the
line. With the center mouse button or with the [Esc] key you can
terminate the measurement at any time.
Warning The image you are using for drawing the line has to be acquired
through the ADDA interface. Otherwise an error message will be
generated.

Warning For drawing the line you can use an image that has not been
acquired through the same channel as the EDX measurement you
are aiming at. In this case please take care that both channels have
the same image size (in pixels) defined. Otherwise an error message
will be generated because the calibrations are not identical and the
wrong pixels would be measured.

EDX Polygon Scan


Press the button EDX Polygon Scan to measure the intensity profile
along a freely definable polygon. In contrast to the command
Linescan you have to enter not a single line, but the entire polygon
into the image in the active image buffer. Mark each corner of the
polygon by clicking at the appropriate pixel with the left mouse button.
Select the last point with a right mouse click. The locations defined
this way are also the locations for the EDX acquisition. The EDX
counts will now be measured, and analySIS® automatically generates
a table with the measurement values. You can use the center mouse
button or the [Esc] key to terminate the measurement at any time.
Warning The warnings given above for the EDX Linescan command also
apply to the EDX Polygon Scan command.

17