Académique Documents
Professionnel Documents
Culture Documents
• PPE and clothing; eq. shoe covers, gloves, disposable tyvek suit
• Disposable
Di bl laboratory
l b t materials
t i l (including
(i l di paper andd plastic l ti waste)
t )
• Infectious microorganisms and toxins and contaminated materials
• Animal origin materials
• Sharps; eq. needles
• Laboratory equipment; eq. rotor’s centrifuge, pipettes
• Spill clean up materials and equipments
• Waste water, including from hand wash, eye wash and shower
• Filters and air handling systems
• Air
Disinfection and Decontamination
Definition:
Decontamination
D t i ti - The destruction
destr ction or removal
remo al of microorganisms
to some lower level, not necessarily zero. (Also applies to removal
or neutralization of toxic agents).
¾ Heat Treatment/Autoclaving
• Moist heat or dry heat
¾ Chemical
• Phenolic,
Phenolic Chlorine,
Chlorine Alcohol
¾ Gaseous
• Formaldehyde
National Institutes
of Health
Treatment of various waste
Incinerator
Process Temperature Laboratory Use
Pasteurization <100°C NO
*Altitude Dependent
Temperature: 121 C • Temperature: 134 C
Pressure: 103 KPa • Pressure: 186 KPa
Time: 15 minutes • Time: 33-10
10 minutes
Process Equipment Temperature Time