SAN PEDRO COLLEGE

Davao City

MEDICAL LABORATORY SCIENCE DEPARTMENT

COURSE OUTLINE

I. COURSE CODE/TITLE: MLS 323 – Analysis of Urine and Other Body Fluids

II. CREDITS: 4 Units (3 hours Lecture and 3 hours Laboratory)

54 hours lecture/semester and 54 hours laboratory/semester

III. COURSE DESCRIPTION:

The course deals with the study of the gross, chemical and microscopic
analyses of the different body fluids other than blood. It includes the importance of
these body fluids to body processes; the principles of the analytical procedures;
interpretation of results and clinical significance of the physiologically important
substances found in these body fluids. Quality Assurance and Laboratory Safety are
also given emphasis.

IV. PRE-REQUISITES:
Biochemistry (CHEM 300M) and Human Anatomy and Physiology (MLS 100)

PLACEMENT: First Semester, 4th Year Level

V. FACULTY:
Edilon A. Liwag, RMT, MSERM
Noraine Princess G. Tabangcora, RMT

VI. CONTACT NUMBERS: (082) 2210257 Local 1173

VII. VISION: San Pedro College, a Dominican learning community, living and spreading
the love of the compassionate Jesus, Healer and Teacher, upholds its core values
serving the poor and disadvantaged, the Filipinos and the world.

MISSION: To provide a nurturing environment beneficial in the total formation of

persons.

CORE VALUES:
 Truth and wisdom
 Excellence and quality
 Family of spirit and sense of caring
 Respect for the uniqueness of persons
 Social responsibility

VIII. GENERAL OBJECTIVES:

At the end of the course, the 3rd year Bachelor in Medical Laboratory Science
students are able to:

1. understand the principles and mechanisms involved in the formation, composition and
general characteristics of different body fluids and secretions
2. perform the routine and special laboratory analyses of body fluids
3. recognize the importance of accurate and precise laboratory findings as aid to the
diagnosis and treatment of disease
4. recognize normal test results and correlate abnormal findings with pathologic conditions
5. manifests medical technologist’s personal, social and professional responsibilities in
helping restore or maintain the health of men with high moral and ethical standards.

Page 1 of 16
 PRELIMINARY PERIOD
WEEK CONTENT SPECIFIC OBJECTIVES
LECTURE: LECTURE:

UNIT ONE UNIT ONE

Introduction Introduction

1. Importance of urinalysis At the end of this unit, the student is

2. QA/QC and Laboratory Safety able to:
3. Competencies & Responsibilities of Medical 1. Rationalize the importance of urine
Technologists in the Clinical Microscopy section examination
4. Definition of terms 2. Discuss the competency requirements
& responsibilities of a Medical
Technologist in the Clinical
Microscopy section
3. Define terms related to urinalysis and
study of body fluids
1
(3 hours) LABORATORY: LABORATORY:

Learning Activity No. 1 Learning Activity No. 1

The Proper Collection and Observation of an The Proper Collection and
Ideal Urine Specimen Observation of an Ideal Urine
for Urine Analysis with Physical Specimen
Examination for Urine Analysis with Physical
Examination

At the end of this laboratory exercise,

the student is expected to:
1. Observe the accurate way of labelling
a urine specimen
2. Identify the type of specimen
3. Assess the type of urine container if
it agrees with the standards of an
ideal urine container
4. Assess the urine specimen if it is
viable for examination
5. Observe the color of the urine
specimen
6. Perform the different methods in
determining the urine specific gravity
using refractometer, hydrometer and
reagent strip

LECTURE LECTURE

UNIT TWO UNIT TWO

Anatomy and Physiology Anatomy and Physiology

1. Review of the Anatomy & Physiology of At the end of this unit, the student is
Kidney able to:
2. Process of Urine Formation 1. Review of the anatomy & physiology
2.1 Filtration of the kidney
2.2 Reabsorption 2. Discuss the process of urine
2.3 Excretion
formation
3. Renal Function Tests
3.1 Creatinine Clearance Test
2
(3 hours) LABORATORY LABORATORY

Learning Activity No. 1 (continuation) Learning Activity No. 1

The Proper Collection and Observation of an (continuation)

Page 2 of 16
 Ideal Urine Specimen The Proper Collection and
for Urine Analysis with Physical Observation of an Ideal Urine
Examination Specimen
for Urine Analysis with Physical
Examination

Students will perform the whole activity

on their own and output will be assessed
by the instructor afterwards.

LECTURE LECTURE

UNIT THREE UNIT THREE

Specimen collection and handling Specimen collection and handling

1. Transport , handling, and disposal
2. Methods of urine preservation
3. Types of urine sample and test/s performed
on each
4. Criteria in accepting urine sample
3
(3 hours) LABORATORY
At the end of this unit, the student is
able to:
1. Discuss patient preparation, proper
specimen collection, transport,
handling, preservation and disposal.
2. Correlate the different types of urine
sample with tests performed.
3. Characterize an acceptable urine
sample
LABORATORY

Learning Activity No. 2 Learning Activity No. 2

The Proper Technique of Performing The Proper Technique of Performing
Chemical Examination of Urine Using Chemical Examination of Urine
Reagent Strip and Conventional Methods Using Reagent Strip and
a. pH Conventional Methods
b. protein a. pH
c. glucose b. protein
d. ketone c. glucose
e. blood d. ketone
e. blood

At the end of this laboratory exercise,

the student is expected to:
1. Describe the proper technique for
performing urine reagent strip tests.
2. Compare the results of the reagent
strip reaction with urine properly
against the manufacturer’s chart.
3. Compare the Glucose reagent strip
test and the conventional method
such as copper reduction test for
sugars in the urine like Benedict’s
test.
4. Compare the Protein reagent strip test
and the conventional method such as
sulfosalicylic acid test for protein in
the urine.
5. Compare the Ketone reagent strip test
and the conventional method such as
Rothera’s test for ketone bodies in
the urine.
6. Compare the Bilirubin reagent strip
test and the conventional method
such as Gmelin’s test for bile

Page 3 of 16
 pigments in the urine.
7. Identify sources of error in
performing the chemical examination
for urine.
8. Define expected normal values for
each parameter.
9. Identify and differentiate normal
results from abnormal results.
10. Correlate results of chemical tests
with those of Physical Examination
of urine.
11. State the chemical principles of the
reagent strips for urine.
12. Summarize the clinical significance
of the reagent strips performed.
13. Suggest causes for abnormal
findings.
14. Cite possible causes of reagent strip
pad deterioration.
15. State the proper care and storage of
reagent strips.

LECTURE LECTURE

UNIT FOUR UNIT FOUR

Physical examination of urine Physical examination of urine

1. Color At the end of this unit, the student is

2. Transparency able to:
3. Volume 1. Perform the physical/macroscopic
4. Specific Gravity urine examination
2. Correlate abnormal macroscopic test
results with pathologic conditions
4
(3 hours) LABORATORY LABORATORY

Learning Activity No. 2 (continuation) Learning Activity No. 2

The Proper Technique of Performing (continuation)
Chemical Examination of Urine Using The Proper Technique of Performing
Reagent Strip and Conventional Methods Chemical Examination of Urine
a. bilirubin Using Reagent Strip and
b. urobilinogen Conventional Methods
c. nitrite
d. leukocyte esterase At the end of this laboratory exercise,
e. specific gravity the student is expected to:
1. Describe the proper technique for
performing urine reagent strip tests.
2. Compare the results of the reagent
strip reaction with urine properly
against the manufacturer’s chart.
3. Compare the Glucose reagent strip
test and the conventional method
such as copper reduction test for
sugars in the urine like Benedict’s
test.
4. Compare the Protein reagent strip test
and the conventional method such as
sulfosalicylic acid test for protein in
the urine.
5. Compare the Ketone reagent strip test

Page 4 of 16
 and the conventional method such as
Rothera’s test for ketone bodies in
the urine.
6. Compare the Bilirubin reagent strip
test and the conventional method
such as Gmelin’s test for bile
pigments in the urine.
7. Identify sources of error in
performing the chemical examination
for urine.
8. Define expected normal values for
each parameter.
9. Identify and differentiate normal
results from abnormal results.
10. Correlate results of chemical tests
with those of Physical Examination
of urine.
11. State the chemical principles of the
reagent strips for urine.
12. Summarize the clinical significance
of the reagent strips performed.
13. Suggest causes for abnormal
findings.
14. Cite possible causes of reagent strip
pad deterioration.
15. State the proper care and storage of
reagent strips

LECTURE LECTURE

UNIT FIVE UNIT FIVE

Chemical examination of urine Chemical examination of urine

1. Routine and Special At the end of this unit, the student is

2. Tube Method able to:
3. Use of strips 1. Discuss the principle involved in
3.1. Protein each of the chemical tests.
3.2. Sugar 2. Perform routine and special chemical
3.3. Bilirubin examination of urine.
3.4. Occult
3. Globin Development
blood/Hemoglobin
3.5. Urobilinogen
3.6. Urobilin
3.7. Nitrites
3.8. Leukocyte
esterase
3.9. Others

5
(3 hours) LABORATORY LABORATORY

Learning Activity No. 2 (continuation) Learning Activity No. 2

The Proper Technique of Performing
Chemical Examination of Urine
Using Reagent Strip and Conventional
(continuation)
The Proper Technique of Performing
Chemical Examination of Urine
Using Reagent Strip and
Conventional

Students will perform the whole activity

on their own and output will be assessed
by the instructor afterwards.

Page 5 of 16
 LECTURE LECTURE

UNIT SIX UNIT SIX

Microscopic examination of urine Microscopic examination of urine

1. Organized & Unorganized Sediments At the end of this unit, the student is
2. Parasites able to:
1. Perform the microscopic urine
examination
2. Describe the microscopic structures
seen in normal and abnormal urine
samples
3. Correlate microscopic test results
with pathologic conditions
6 LABORATORY LABORATORY
(3 hours)
Written and Practical Exam Written and Practical Exam

Students will take written and practical

periodic laboratory examination.
TOTAL: 18 HOURS

MIDTERM PERIOD
WEEK CONTENT SPECIFIC OBJECTIVES
LECTURE LECTURE

UNIT SEVEN UNIT SEVEN

Renal diseases Renal diseases

1. Glomerular and Tubular Renal Disorders At the end of this unit, the student is
2. Renal Calculi able to:
3. Aminoacidurias 1. Discuss the different renal diseases
4. Reference Values 2. Discuss the physical and chemical
5. Clinical Significance examination of renal calculi
6. Reporting of Test Results 3. Discuss the different amino acid
disorders
4. Correlate test results with pathologic
conditions
5. Recognize normal test results in
urine examination
6. Apply quality assurance in urine
examination
7. Report correct test results using
standard format of reporting
1
(3 hours) LABORATORY LABORATORY

Learning Activity No. 3 Learning Activity No. 3

The Proper Way To Identify And Report The Proper Way To Identify And
Different Urinary Sediments Report Different Urinary Sediments

At the end of this laboratory exercise,

the student is expected to:
1. Review on the use of Brightfield
Microscope and its use in the
microscopic examination of urine
2. Describe the procedure for proper
microscopic examination of urine
3. Identify and differentiate urinary
sediments and artifacts
4. Identify cells that may be found in
urinary sediment

Page 6 of 16
 5. Identify crystals that may be found in
acidic and alkaline urine urine
6. Identify casts that may be found in
urinary sediment
7. Identify microorganisms that may be
found in urinary sediment
8. Correlate presence of urine sediment
with results of physical and chemical
analysis
9. Learn the appropriate manner of
reporting the different urinary
sediments

LECTURE LECTURE
2
UNIT EIGHT UNIT EIGHT
Examination of body fluids - Examination Examination of body fluids -
of Cerebrospinal Fluid Examination of Cerebrospinal
Fluid
1. Definition of Terms
2. Importance of Cerebrospinal Fluid analysis At the end of this unit, the student is
3. Process of formation & elimination able to:
4. Methods and sites of collection & 1. Define terms
precautions involved 2. Explain the process of CSF
5. Transport, handling, processing, formation
preservation & disposal
3. Describe the collection, transport,
handling, processing, preservation
and disposal of sample
4. Describe an acceptable CSF sample
for routine examination
5. Characterize a normal CSF
6. Discuss the importance of CSF
analysis
7. Perform routine CSF examination
8. Discuss principle involved of each
test performed on CSF
9. Classify bacterial, viral and fungal
meningitis based on test results
10. Correlate abnormal test results with
pathologic findings
11. Discuss variables that affect test
results
12. Apply concepts of quality assurance
and quality control
13. Report the test results based on the
standard format
2
(3 hours) LABORATORY LABORATORY

Learning Activity No. 3 (continuation) Learning Activity No. 3

The Proper Way To Identify And Report (continuation)
Different Urinary Sediments The Proper Way To Identify And
Report Different Urinary Sediments

Students will perform the whole activity

on their own and output will be assessed
by the instructor afterwards.

Page 7 of 16
 LECTURE
UNIT NINE
Examination of body fluids - Examination
of Cerebrospinal Fluid (continuation)
6. Acceptable CS.F sample
7. Characteristic of a normal CSF
8. Routine Examination
8.1 Macroscopic
8.2 Microscopic
8.3 Chemical : Routine and Special
8.4 Gram stain and Culture
8.5 Serologic Test (e.g. VDRL)
3
(3 hours) LABORATORY
Learning Activity No. 3 (continuation)
The Proper Way To Identify And Report
Different Urinary Sediments
LECTURE
UNIT TEN
Examination of body fluids - Examination
of Cerebrospinal Fluid (continuation)
9. Reference Values
10. Referral of specimens
4 11. Clinical Significance: Bacterial, Viral,
(3 hours) Fungal
12. Sources of error
13. Quality Assurance/quality control
14. Reporting of test results
LECTURE
UNIT NINE
Examination of body fluids -
Examination of Cerebrospinal
Fluid (continuation)
At the end of this unit, the student is
able to:
1. Define terms
2. Explain the process of CSF
formation
3. Describe the collection, transport,
handling, processing, preservation
and disposal of sample
4. Describe an acceptable CSF sample
for routine examination
5. Characterize a normal CSF
6. Discuss the importance of CSF
analysis
7. Perform routine CSF examination
8. Discuss principle involved of each
test performed on CSF
9. Classify bacterial, viral and fungal
meningitis based on test results
10. Correlate abnormal test results with
pathologic findings
11. Discuss variables that affect test
results
12. Apply concepts of quality assurance
and quality control
13. Report the test results based on the
standard format
LABORATORY
Learning Activity No. 3
(continuation)
The Proper Way To Identify And
Report Different Urinary Sediments
Students will perform the whole activity
on their own and output will be assessed
by the instructor afterwards.
LECTURE
UNIT TEN
Examination of body fluids -
Examination of Cerebrospinal
Fluid (continuation)
At the end of this unit, the student is
able to:
1. Define terms
2. Explain the process of CSF
formation
3. Describe the collection, transport,
handling, processing, preservation
and disposal of sample
4. Describe an acceptable CSF sample
Page 8 of 16
 for routine examination
5. Characterize a normal CSF
6. Discuss the importance of CSF
analysis
7. Perform routine CSF examination
8. Discuss principle involved of each
test performed on CSF
9. Classify bacterial, viral and fungal
meningitis based on test results
10. Correlate abnormal test results with
pathologic findings
11. Discuss variables that affect test
results
12. Apply concepts of quality assurance
and quality control
13. Report the test results based on the
standard format

LABORATORY LABORATORY

Learning Activity No. 4 Learning Activity No. 4

The Proper Way To Perform And Report The Proper Way To Perform And
One-Step Qualitative Urine Pregnancy Test Report One-Step Qualitative Urine
Pregnancy Test

At the end of this laboratory exercise,

the student is expected to:
1. Discuss the principle of qualitative
urine pregnancy test
2. Discuss the significance of
performing Fecal Occult Blood Test
3. Properly instruct a patient on how to
collect samples for pregnancy testing
4. Demonstrate step-wise procedure of
One-Step Qualitative Urine
Pregnancy Test
5. Properly report and interpret
Qualitative Urine Pregnancy Test
results
6. Identify limitations of performing the
procedure.

LECTURE LECTURE

UNIT ELEVEN UNIT ELEVEN

Examination of body fluids - Examination Examination of body fluids -
of Seminal Fluid Examination of Seminal Fluid

At the end of this unit, the student is

1. Definition of terms able to:
2. Formation of Seminal Fluid 1. Define terms
3. Patient preparation 2. Discuss formation of seminal fluid
4. Specimen collection, handling, transport, 3. Enumerate the patient requirements
preservation & processing 4. Discuss methods of collection,
5
5. Acceptable sample transport, handling, processing,
(3 hours) 6. Routine Examination
6.1. Macroscopic preservation and proper disposal
6.2. Liquefaction time 5. Characterize an acceptable specimen
6.3. Viability test for routine testing
6.4. Motility 6. Describe a normal seminal fluid
6.5. Cell count & differential count 7. Perform a routine semen analysis
6.6. Chemical examination 8. Explain the significance of seminal

Page 9 of 16

LABORATORY
Learning Activity No. 4 (continuation)
The Proper Way To Perform And Report
One-Step Qualitative Urine Pregnancy Test
LECTURE
UNIT TWELVE
Plasma Cell Dyscrasia
7. Special Examinations
8. Reference Values
9. Clinical Significance of Seminal fluid
analysis: Infertility & Vasectomy
10. Sources of error
11. Quality assurance/quality control
12. Reporting of test results
6
(3 hours) LABORATORY
Written and Practical Exam
TOTAL: 18 HOURS
fluid analysis
9. Discuss principle involved in each
test performed
10. Correlate test results with pathologic
conditions
11. Discuss variables that affect test
results
12. Apply concepts of quality assurance
and quality control
13. Report test results based on the
standard format
LABORATORY
Learning Activity No. 4
(continuation)
The Proper Way To Perform And
Report One-Step Qualitative Urine
Pregnancy Test
Students will perform the whole activity
on their own and output will be assessed
by the instructor afterwards.
LECTURE
UNIT TWELVE
Examination of body fluids -
Examination of Seminal Fluid
(continuation)
At the end of this unit, the student is
able to:
1. Define terms
2. Discuss formation of seminal fluid
3. Enumerate the patient requirements
4. Discuss methods of collection,
transport, handling, processing,
preservation and proper disposal
5. Characterize an acceptable specimen
for routine testing
6. Describe a normal seminal fluid
7. Perform a routine semen analysis
8. Explain the significance of seminal
fluid analysis
9. Discuss principle involved in each
test performed
10. Correlate test results with pathologic
conditions
11. Discuss variables that affect test
results
12. Apply concepts of quality assurance
and quality control
13. Report test results based on the
standard format
LABORATORY
Written and Practical Exam
Students will take practical and written
periodic laboratory examination.
Page 10 of 16
 FINAL PERIOD
WEEK CONTENT SPECIFIC OBJECTIVES
LECTURE LECTURE

UNIT THIRTEEN UNIT THIRTEEN

Examination of body fluids - Examination Examination of body fluids -
of Amniotic Fluid Examination of Amniotic Fluid

1. Definition of Terms At the end of this unit, the student is

2. Formation and composition of amniotic fluid able to:
3. Patient requirements & preparation 1. Define terms
4. Acceptable sample 2. Discuss formation of amniotic fluid
5. Specimen collection, transport, handling, 3. Discuss guidelines regarding
processing and disposal patients preparation
6. Macroscopic Exam 4. Discuss methods of collection,
7. Tests to determine fetal distress, fetal lung transport, handling, processing &
maturity & HDN proper disposal
8. Reference values 5. Characterize an acceptable
9. Clinical Significance specimen for routine testing
10. Sources of error 6. Describe a normal amniotic fluid
11. Quality Assurance and control 7. Explain the significance of
12. Reporting of test results amniotic fluid analysis
13. Laboratory Safety 8. Discuss principle involved in each
test
9. Correlate test results with
pathologic conditions
10. Discuss variables that affect test
results
11. Apply concepts of quality
assurance and quality control
12. Report the test results based on
the standard format
1
(3 hours) LABORATORY LABORATORY

Learning Activity No. 5 Learning Activity No. 5

The Proper Way To Perform Fecal Occult The Proper Way To Perform Fecal
Blood Test Occult Blood Test

At the end of this laboratory exercise,

the student is expected to:
1. Discuss the principle of the
chemical method for Fecal Occult
Blood Test
2. Enumerate the causes of presence
of blood in feces and discuss the
significance of performing Fecal
Occult Blood Test
3. Properly instruct a patient on how
to collect samples for the Occult
Blood test including dietary
restrictions to follow before testing
4. Demonstrate step-wise procedure
of Fecal Occult Blood Test
5. Identify the substances that will
possibly interfere in the analysis
and results of FOBT

Page 11 of 16
 LECTURE LECTURE

UNIT FOURTEEN LECTURE

Examination of body fluids - Examination
of Transudates and Exudates UNIT FOURTEEN
Examination of body fluids -
1. Definition of terms Examination of Transudates and
2. Formation of these fluids Exudates
3. Patient requirements & preparation
4. Acceptable sample At the end of this unit, the student is
5. Specimen collection, transport, handling, able to:
processing & disposal 1. Define terms
6. Macroscopic exam 2. Discuss formation of these fluids
7. Microscopic exam 3. Characterize transudates and
8. Chemical Examination
exudates
9. Reference values
10. Clinical significance 4. Enumerate the patient requirements
11. Sources of error 5. Discuss methods of collection,
12. Quality assurance and control transport, handling, processing and
13. Reporting of test results proper disposal
14. Laboratory Safety 6. Characterize an acceptable
specimen for routine testing
7. Explain the significance of fluid
analyses
8. Discuss principle involved of each
test performed
9. Correlate test results with
pathologic conditions
10. Discuss variables that affect test
results
11. Apply concepts of quality
assurance and quality control
12. Report test results based on the
standard format
2
LABORATORY LABORATORY
(3 hours)
Learning Activity No. 5 (continuation) Learning Activity No. 5
The Proper Way To Perform Fecal Occult Blood (continuation)
Test The Proper Way To Perform Fecal
Occult Blood Test

Students will perform the whole

activity on their own and output will
be assessed by the instructor
afterwards.

LECTURE LECTURE

UNIT FIFTEEN UNIT FIFTEEN

Examination of body fluids - Examination Examination of body fluids -
of Synovial Fluid Examination of Synovial Fluid

1. Definition of terms At the end of this unit, the student is

2. Formation of synovial fluid
3. Patient requirements & preparation
4. Acceptable sample
5. Specimen collection, transport, handling,
processing & disposal
6. Anticoagulants
7. Macroscopic examination
8. Microscopic examination
able to:
1. Define terms
2. Discuss formation of synovial fluid
3. Enumerate the patient requirements
4. Discuss methods of collection,
transport, handling, processing and
proper disposal
5. Characterize an acceptable

Page 12 of 16
 9. Strings test specimen for routine testing
10. Chemical Tests 6. Describe a normal synovial fluid
11. Serological Tests 7. Explain the significance of
12. Gram stain and culture synovial fluid analyses
13. Reference values 8. Discuss principle involved of each
14. Clinical significance test performed
15. Sources of error 9. Correlate test results with
16. Quality assurance/quality control pathologic conditions
17. Reporting of test results 10. Discuss variables that affect test
18. Laboratory Safety results
11. Apply concepts of quality
assurance and quality control
12. Report the test results based on
the standard format
3
(3 hours) LABORATORY LABORATORY

Learning Activity No. 6 Learning Activity No. 6

The Proper Way To Perform the Collection, The Proper Way To Perform the
Storage, Transport and Analysis of Seminal Collection, Storage, Transport and
Fluid Analysis of Seminal Fluid

At the end of this laboratory exercise,

the student is expected to:
1. Explain the importance of
performing seminal fluid analysis.
2. Explain the proper collection and
specimen handling of seminal fluid
samples.
3. Describe procedures for
macroscopic and microscopic
analysis of semen.
4. Identify normal and abnormal
values for tests comprising a
routine semen analysis.
5. Identify and describe normal and
abnormal spermatozoa
morphologies.
6. Identify and describe various
sperm motilities.
7. Identify and describe the
appearance of viable and nonviable
spermatozoa.
8. Demonstrate step-wise procedure
of Semen Analysis
9. Correlate results of semen analysis
tests.

LECTURE LECTURE

UNIT SIXTEEN UNIT SIXTEEN

Qualitative Platelet Disorders
1. Definition of terms
2. Formation of these fluids
3. Patient requirements & preparation
4. Acceptable sample
5. Specimen collection, transport, handling,
processing & disposal
6. Macroscopic exam
4 7. Microscopic exam
(3 hours) 8. Test for Total Acidity, Free HACL etc.
9. Reference values
10. Clinical significance
11. Sources of error
12. Quality assurance/quality control
Examination of body fluids -
Examination of Gastric Juice and
Duodenal Contents
At the end of this unit, the student is
able to:
1. Define terms
2. Discuss formation of gastric juice
and other duodenal contents
3. Enumerate the patient requirements
4. Discuss methods of collection,
transport, handling, processing &
proper disposal
5. Characterize an acceptable
specimen for routine testing
6. Describe normal gastric juice and

Page 13 of 16
 13. Reporting of test results
14. Laboratory Safety
duodenal contents
7. Explain the significance of fluid
analyses
8. Discuss principle involved of each
test performed
9. Correlate test results with
pathologic conditions
10. Discuss variables that affect test
results
11. Apply concepts of quality
assurance and quality control
12. Report the test results based on
the standard format

LABORATORY LABORATORY

Learning Activity No. 6 (continuation) Learning Activity No. 6

The Proper Way To Perform the Collection, (continuation)
Storage, Transport and Analysis of Seminal The Proper Way To Perform the
Fluid Collection, Storage, Transport and
Analysis of Seminal Fluid

Students will perform the whole

activity on their own and output will
be assessed by the instructor
afterwards.
LECTURE LECTURE

UNIT SEVENTEEN UNIT SEVENTEEN

Examination of body fluids - Examination Examination of body fluids -
of Sputum Examination of Sputum

1. Definition of terms At the end of this unit, the student is

2. Formation of sputum able to:
3. Patient requirements & preparation 1. Define terms
4. Acceptable sample 2. Discuss formation of sputum
5. Specimen collection, transport, handling, 3. Enumerate guidelines regarding
processing & disposal patients preparation
6. Routine examination 4. Discuss methods of collection,
6.1. Macroscopic exam transport, handling, processing,
6.2. Microscopic exam (cells, parasites, etc.) preservation & proper disposal
6.3. Gram stain 5. Characterize an acceptable
6.4. Acid fast stain specimen for routine testing
7. Reference values 6. Describe a normal sputum
8. Clinical significance 7. Explain the significance of fluid
9. Sources of error analyses
10. Quality assurance/quality control 8. Discuss principle involved of each
11. Reporting of test results test performed
9. Perform sputum examination.
10. Correlate test results with
pathologic condition
11. Identify sources of error & effect
on test results
12. Apply concepts of quality
assurance and quality control
13. Report test results based on the
5 standard format
(3 hours) LABORATORY LABORATORY

Return demonstration on manual urinalysis Return demonstration on

manual urinalysis

Students will perform the whole

activity on their own and output will
be assessed by the instructor
afterwards.

Page 14 of 16
 LECTURE LECTURE

UNIT EIGHTEEN UNIT EIGHTEEN

Examination of body fluids - Examination Examination of body fluids -
of Feces Examination of Feces

1. Definition of Terms At the end of this unit, the student is

2. Patient Preparation
3. Specimen Collection, processing,
preservation, handling and disposal
6
4. Acceptable stool specimen & prepared
(3 hours) smear
5. Chemical Examination: Routine & Special
6. Clinical Significance
7. Sources of error
8. Quality assurance/quality control
9. Reporting of test results
able to:
1. Define terms
2. Discuss patient preparation
3. Describe methods of collection,
proper transport, preservation,
handling and disposal
4. Characterize acceptable stool
specimen for routine examination
5. Perform routine and special
chemical examination
6. Discuss chemical constituents of
stool according to source: clinical
significance, methods used for
analysis and principle involved of
each test
7. Correlate test results with
pathologic conditions
8. Discuss variables that affect test
results
9. Apply concepts of quality
assurance
10. Report correct test results using
the standard format

LABORATORY LABORATORY

Written and Practical Exam Written and Practical Exam

Students will take practical and

written periodic laboratory
examination.
TOTAL: 18 HOURS

IX. EVALUATION/ GRADING SYSTEM:

LECTURE: Periodic Examination 50%
Quizzes 40%
Others 10%(Recitation/Detailing
Presentation, Assignments, Seatwork,
Attendance)

LABORATORY: Long Examinations 50%

Quizzes 30%
Others 20% (Laboratory Performance,
Reports, Output, Attendance, Return
Demonstration)

X. COURSE REQUIREMENTS
1. Satisfactory grades in quizzes and examinations.
2. Completion and submission of the prescribed number of laboratory
experiments.
3. Completion of requirements or projects related to the subject.

Page 15 of 16
 XI.
REVISION REVISION PREPARED BY: NOTED BY: APPROVED BY:
STATUS: DATE:

Fourth August 8, Florie May M. Enanoria, Geromil J. Josephine M.

Revision 2017 RMT Lara, RMT, Bandalan, MD,
MSMT MS
Program Dean
Coordinator

XII. REFERENCES:

1. Brunzel N.A. Fundamentals of Urine and Body Fluids Analysis. Pennsylvania:

Saunders, 2004.

2. Mcpherson, Richard A. and Matthew R. Pincus. Henry’s Clinical Diagnosis and

Management by Laboratory Methods 21st ed. Philadelphia: Elsevier Inc., 2007.

3. Mundt, L.A and Shanahan, K. Graff’s Textbook of Routine Urinalysis and Body
Fluids 2nd ed. Philadelphia: Lippincott Williams and Wilkins, 2011.

4. Strasinger S.K. and Di Lorenzo M.S, Urinalysis and Body Fluids 5thed. USA: F.A.
Davis Company, 2008.

* References related to Analysis of Urine and Body Fluids that are available in the
library may also be used.

Page 16 of 16