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Abstract Book

Sixth International Conference

Plants and Environmental Pollution

27-30 November, 2018

Organised by
International Society of Environmental Botanists
CSIR-National Botanical Research Institute
Lucknow, INDIA

Sixth International Conference

Plants and Environmental Pollution

27-30 November, 2018

Organised by
International Society of Environmental Botanists
CSIR-National Botanical Research Institute
Lucknow, INDIA
Publication Committee
Dr. P.A. Shirke

Dr. Vidhu Sane


Prof. R.S. Tripathi

Dr. R.D. Tripathi
Dr. Nandita Singh
Dr. Vivek Pandey
Dr. Seema Mishra
Dr. Amit Kumar
Dr. K.N. Nair
Dr. P.C. Abhilash
Dr. Sudhakar Srivastava
Dr. Anjum Farooqui
Dr. Pankaj K. Srivastava
Dr. Soumit K. Behera
Dr. Shekhar Mallick
Dr. K.K. Rawat
Dr. Farah Deeba
Mr. Rajat R. Rastogi
Mr. Dilip Kumar Chakraborty
Mr. D.B. Shukla

Organizing Secretaries
Dr. R.D. Tripathi Dr. Nandita Singh Dr. Vivek Pandey

E nvironmental pollution is increasing exponentially with each passing season due to drastically
changing human needs. The "Sixth International Conference on Plants and Environmental
Pollution" (ICPEP-6), to be held in Lucknow, India, would be the venue when the luminaries of
Environmental Science across the world would assemble to discuss and deliberate on the pressing
environmental issues, and lay the path for future course of actions and research in the area of Plants and
Environmental Pollution.
The "Sixth International Conference on Plants and Environmental Pollution" (ICPEP-6), was able
to kindle for submission of about 413 abstracts for the four day event. These abstracts have been
compiled into this "Abstract Book" which we received from the distinguished in the area of
Environmental science to the novice research scholars, however, with gleam of new ideas.
The abstracts included in this volume were received within the prescribed time limit. All the
submissions were peer reviewed and edited and classified into the eight themes of the conference and
the abstracts were segregated in different sessions for oral and poster presentation. This time there was a
lot of zeal from the young researchers for the oral presentation, keeping their enthusiasm in mind, the
ratio of oral presentations were increased as compared to the last conference with opportunities to the
young minds as well as to the peers of the respective sessions.
We place on record our deep sense of appreciation to Prof. S.K. Barik, Director CSIR-NBRI and
President ISEB, for his whole hearted support in terms of logistic and expert opinion. We are thankful to
Drs. R.S. Tripathi, P.V. Sane and K.J. Ahmad for their timely apt suggestions and encouragements. We
are beholden to our contributors who regardless of their various assignments and commitments
acceded to our request to commit their valuable time and expertise.
We are thankful to the Scientific Program Committee for categorizing the abstracts and editing
them. We are also thankful to the staff of ISEB Secretariat for their co-operation in various ways.
We are indebted to the various sponsoring agencies for financial support which made this
publication accomplishable. Finally the efforts of M/S Army Printing Press and their team
metamorphosed the publication to the present form, we sincerely thank these people.

Publication Committee
Scientic Programme Committee
Prof. R.S. Tripathi

Dr. R.D. Tripathi

Prof. R.P. Singh
Dr. O.P. Dhankher (USA)
Dr. F.J. Corpas (Spain)
Dr. Nandita Singh
Dr. Vivek Pandey
Dr. Prabodh Trivedi
Prof. G.S. Singh
Dr. Debasis Chakrabarty
Dr. Sanjay Dwivedi
Dr. Anjum Farooqui
Dr. Amit Kumar Gupta
Prof. M. Iqbal
Dr. S.N. Jena
Prof. Kumkum Mishra
Dr. Amit Kumar
Prof. Sheshu Lavania
Dr. Shekhar Malick
Dr. Seema Mishra
Dr. Soumit K. Behera
Dr. Pankaj Kumar Srivastava
Dr. P. Suprasanna
Dr. Preeti Tripathi
Special Lectures : 1
Session S-I : Climate Change: Adaptation, Mitigation,
Carbon Sequestration and Food Security 5

Session S-II : Biodiversity: Prospection and Conservation 33

Session S-III : Environmental Biotechnology and Microbiology 71

Session S-IV : Environmental Impact Assessment 103

Session S-V : Sub-tropical and Tropical Oak under Global Change 113

Session S-VI : Biomonitoring and Bioremediation 129

Session S-VII : Plant Response to Environmental Pollution 175

Session S-VIII : Contemporary Issues in Environment and Plant 215

Author Index 241

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Special Lectures

Abstracts 27-30 November, 2018, NBRI, Lucknow 1

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

2 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SL-1 severe water stress will increase to 4 billion by 2025.

350 million ha of tropical forests have been deforested
Environmental Crisis (0.8% per year) and 500 million ha of secondary and
primary forests have been degraded, affecting the
J.S. Singh* generation and flow of ecosystem services vital for
Department of Botany, Banaras Hindu University, Varanasi- human well-being. Biodiversity is the underpinning of
221005, INDIA, Email: singh.js1@gmail.com ecosystem services. These services include (i)
provisioning Services- goods such as food, fresh water,
The Earth today is experiencing environmental
wood and fibre, (ii) regulating services- disease control,
conditions which it has never experienced in the past.
climate regulation, water purification, flood regulation,
For example, the CO2 concentration has varied only
etc., (iii) cultural services- education, recreation, etc.,
between 180 to 280 ppm during the past 420,000 years
and (iv) supporting services-nutrient cycling, soil
but it has now increased to >400ppm. This human-
formation, primary productivity, etc. Fourteen out of
dominated era which started since 1850 is now termed
identified 24 services are now declining. According to
Anthropocene. The Living Planet Index (biocapacity
one estimate, the current economic value of the 17
of the Earth) fell by 56 per cent and Ecological Footprint
ecological services for 16 biomes is in the range of US
(area of biologically productive land or sea required to
$ 16–54 trillion per year. Studies indicate that we have
produce food, material and energy) exceeded the
entered into a phase of mass extinction, today we seem
Earth’s biocapacity by 50%. The anthropogenic
to be losing two to five species per hour from tropical
activities, to a great extent, are responsible for bringing
forests alone. This amounts to a loss of 16 million
about global climate change. Predictions indicate that
species populations per year or 1800 populations per
the average temperature of the Earth’s near surface
hour. Artificial light is emerging as an important threat
could increase by 5.8°C, and sea level may rise by an
to biodiversity, particularly in coastal areas, which can
additional 88 cm by the year 2100. The total temperature
increase prey intake by wading shorebirds, resulting in
increase from 1850-1899 to 2001-2005 is 0.76 [0.57 to
the depletion of local invertebrate densities, in the long
0.95]°C, and the rate of sea level rise was 1.8 mm/yr
term, and certain nesting sea turtles are more
between 1961-2003, and 3.1 mm/yr between 1993-
predominant in dark areas relative to well-lit coastal
2003. Using a Systems approach focusing on
feedbacks, tipping points and non-linear dynamics,
scientists have postulated that the Earth System may Key words: Environment, Biodiversity, Ecosystem, Climate.
be approaching a planetary threshold and a rapid
human-driven pathway towards a much hotter SL-2
conditions- the hothouse earth (away from the glacial-
interglacial cycles) and a changed biosphere, when
Biodiversity: A Vital Resource for
parts of the earth can be devoid of human population. Civilization, Economic Development
Important feedbacks that could maintain the earth and Wellbeing
system in Holocene-like conditions are weakening
relative to human forcing but the Earth System has to C.K. Varshney
be maintained in habitable, interglacial-like state. For
Professor Emeritus and Distinguished Adjunct Professor
India, predictions include: frequent dry days, 38% drop
(AIT, Bangkok.), School of Environmental Science,
in per capita water availability by 2050, 50 million people
Jawaharlal Nehru University, New Delhi-110067, INDIA,
would be displaced in coastal area due to sea level rise Email: ckvarshney@hotmail.com
(two islands in Sunderbans are already submerged),
by 2035 food grain production may fall by 30%, vector Biodiversity is the most complex feature of our
borne diseases and those associated with floods and planet and extremely vital for human society. It refers
droughts will increase, and 25% flora and fauna may to diversity of living organism-plants, animals and
be at a high risk of disappearance by 2030. World- microorganisms- at multiple scales of biological
wide, 2.2 billion people now living under moderate to organization (genes, populations, species, and
ecosystems) and can be considered at any geographic

Abstracts 27-30 November, 2018, NBRI, Lucknow 3

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

scale -local, regional, or global. Biodiversity has evolved under cared and often taken for granted. Wide spread
over millennia, is inherently dynamic, multidimensional, deforestation, loss of habitat, growing pollution and
omnipresent and, regarded to have transformed earth’s climate change are serious threats to biodiversity. The
primeval atmosphere into oxygen rich atmosphere and current rate of global diversity loss is estimated to be
more importantly it is only system that harvest and 100 to 1000 times higher over the background extinction
stores solar energy at a planetary scale. Humankind is rate. It is estimated that half of all the species on the
obligately dependent on biodiversity for food, breath, planet could go extinct by 2050- only 32 years from
medicine and other basic needs and wellbeing. The now. Biodiversity loss is a crisis of historical and
nexus between biodiversity and human civilization is planetary proportions. Biodiversity loss may amplify
the single most important factor responsible for climate warming, and could lead to additional,
sustaining human survival, scientific and industrial unforeseen, and potentially irreversible shifts in the
progress, economic growth and cultural evolution. earth system. Despite numerous commitments,
Biodiversity viewed in the context of sustainable biodiversity loss continues to accelerate in all
development offers opportunities for poverty regions.The situation is alarming and unless urgent
eradication, human well-being and livelihood. In spite action taken now, poverty eradication, food and water
rapid stride in the growth of science and technology security and sustainable future will remain elusive.
human dependence on biodiversity is rising and likely Key words: Biodiversity, Pollution, Climate change,
to remain indispensable in foreseeable future. Extinction.
Unfortunately, biodiversity is grossly undervalued and

4 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Session I
Climate Change: Adaptation, Mitigation,
Carbon Sequestration and Food Security

Abstracts 27-30 November, 2018, NBRI, Lucknow 5

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

6 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SI/KN-1 such challenges are that the given genotype/s must be

seed infertile to realize non-invasive features, must have
Challenges and Chances to Get More low oil in its roots to serve as deterrent to root diggers,
Crop per Drop by using Abscisic Acid should sport deep penetrating web -forming root system
to facilitate soil binding, and root architecture facilitating
Receptors high heavy metal absorbing capacity to rehabilitate
toxicity of the degraded soil dumps. Success achieved
Erwin Grill in this direction shall be illustrated to substantiate the
Chair of Botany, Technical University of Munich, utility of vetiver grass as a potential bio resource to
GERMANY, Email: erwin.grill@wzw.tum.de address such environmental concerns.
Plants are ferocious consumers of water. More Key words: Vetiver grass, Phytotechnology, Natural disaster,
than two third of anthropogenic fresh water use is Environmental conservation, Remediation.
channelled into agriculture contributing to social
conflicts over water resources. The atmospheric SI/L-2
carbon dioxide is taken up by plants for biosynthesis of
organic compounds and this carbon influx is inherently Climate Change: Past, Present and
associated with massive efflux of water vapour. The Future: Adaptation and Food Security
lecture will address the mechanisms involved in the
homeostasis of plant’s water status and whether high A.K.M. Nazrul Islam
yield of plants can be combined with reduced water
Ecology and Environment Laboratory, Department of
consumption by harnessing recent insights into the Botany, University of Dhaka, Dhaka, BANGLADESH
control of transpiration by the phytohormone abscisic Email: nazrul.islam.akm@gmail.com
acid (ABA).
The composition of the atmosphere is changing
Key words: Abscisic acid, Plant water status, Water use at an unprecedented rate because of manmade
activities. Earth’s climate has changed before many
times in the last two billion years. But something
important is different at present. The topic will be
Vetiver Plant Type Suitable for Disaster discussed in relation to (i)The Evolution of the Earth
(Past and Present conditions) (ii) Increase of Human
Management in Climate Change Regime Population and its influence (iii) The change of CO2
Concentration in the atmosphere (iv) An attempt to
Umesh C. Lavania explain climate change in relation to adaptation and
Department of Botany, Lucknow University, Lucknow- food security. The ancient and the future continent of
226007, INDIA, Email: lavaniauc@yahoo.co.in the planet and the time span from about 20 kyr BP to
Vetiver grass, Chrysopogon zizaniodes (L.) the present will be discussed. The evidence for climate
Roberty, a perennial C4 grass, industrially valued for and chemistry from several different sources permits
its aromatic essential oil in the roots, has of late to know the environmental conditions in fair detail for
attracted global attention as a natural means for a period substantially longer than that in recorded
multifarious environmental applications including human history. The history of human population growth
detoxification of degraded soil and water. In order to was calculated by extrapolation from census figures
realize enhanced potential of this grass to address that exist for the present day agricultural societies and
environmental concerns, extensive studies are by examination of archaeological remains. Atmospheric
undertaken to identify and / or develop suitable CO2 Concentration has gone both up and down during
genotypes, and extend scope of vetiver phytotechnology the long geologic history of the earth and the human
as a natural means to sequester atmospheric carbon inputs of CO2 are one-way flux to the atmosphere,
deep into subsoil horizon, reduce landslides and mitigate due principally to burning fossil fuel and deforestation,
spoiled soil dumps. The key prerequisites to address and to other land use practices and conversions. The

Abstracts 27-30 November, 2018, NBRI, Lucknow 7

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

total human induced emissions of CO2 were relatively SI/L-3

constant from 1780 to 1860.Recent estimates suggest
that the gross CO2 emissions from deforestation and Caring Our “Green Lungs”:
land use changes during the 1980s are appx.1.6 Gt. of Nurturing and Protecting Urban Trees
C per year. It was concluded that changes in carbon
dioxide is a key factor in climate change and have had and Forests under the Threats of
an important influence on climate, at least for the past Climate Change, Urbanization, and
160 thousand years. Abundance of CO2 was relatively Pollution
high during the warmer Cretaceous and Eocene
epochs. – interrupting measurements of isotopic Somidh Saha1,2 *, Gerhard Sardemann1 , Annika
composition (13C) of organic material from plankton Fricke1, Sarah Meyer-Soylu1, Colette Waitz1, Oliver
preserved in ocean sediments laid down during the Parodi1, Andreas Seebacher 1 , Christine Rösch1 ,
Cretaceous period and concluded that the level of CO2 Jürgen Kopfmüller1, Axel Albrecht3, Bernd Stähler4
during the Cretaceous was 4 to 12 times higher than and Andreas Matzarakis5
today. It appears from the data that a value between
about 600 and 700 ppm may have applied during the Institute for Technology Assessment and System Analysis,
Karlsruhe Institute of Technology, Karlstrasse 11, D-76133
most recent warm period of the Eocene. Evidence
Karlsruhe, GERMANY; 2Chair of Silviculture, University of
showed that temperature has decreased by about 30C
Freiburg, Tennenbacherstrasse 4, D-79085 Freiburg,
over the past 6000 years. From sea level studies of GERMANY; 3Forest Research Institute of Baden-
Bangladesh, it was postulated that an inundation of up Württemberg, Wonnhaldestrasse 4, D-79100 Freiburg,
to one third of the country due to 1m and 3 m rises in GERMANY; 4Garden Department of City of Karlsruhe,
relative sea level by estimating the area below the 1m Lammstrasse 7a, D-76131 Karlsruhe, GERMANY; 5Centre
and 3 m contour lines due to changes in river gradients, for Medical Meteorological Research, German Weather
sedimentation and drainage; the primary impact of Service, Stefan-Meier-Strasse 4, D-79104 Freiburg,
global mean sea level on Bangladesh will be an increase GERMANY, Email: somidh.saha@kit.edu
in flooding in the depressed basins upstream rather than Cities and heavily urbanized areas in Germany
only at the coast or within the tidal limits. The warmest are facing the challenge of preserving their green
temperatures during the past 10,000 years occurred spaces (“green lungs”) under pressure from growing
and the peak temperatures were observed during the population and expanding infrastructure. At the same
period known as Hypsithermal, about 8000 to about time, the vulnerability of urban regions to climate
5000 radiocarbon years from now. Greenhouse gas change-induced extreme weather events such as
emissions by the developing world have committed us drought and heat waves has been increasing. Trees
to a mean sea level rise of only 2.7 to 3.5 cm by 2030 and green spaces in urban areas reduce air pollution,
and 5.0 to 6.5 cm by 2100. Global climate change will mitigate heat stress and provide other ecosystem
increase the stress on agricultural systems, potentially services. But they too are vulnerable to climate change
decreasing yields at the very time when demand for and air pollution (e.g. NOx). Therefore, there is an
food is growing dramatically. The question that requires urgent need to assess the resilience of urban trees and
an answer include: Are we approaching catastrophic forests to climate change and pollution impacts in order
climate change? The ends of the Earth will occur? Will to reduce vulnerability. Significant trade-offs may exist
The planet exist? The answer is very complex and between conflicting management goals in urban trees
uncertain. Future food habit may be changed and forest management which should be minimized
dramatically. through participatory decision making. In this context,
Key words: Climate change, Earth’s evolution, Future participation of civil society, research institutions, local
conditions, Food security. public institutions, and other stakeholders is of vital
importance for future adaptation planning for urban
green areas. In this presentation, we will present an
innovative trans disciplinary concept to increase the

8 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

resilience of urban trees and forests to multiple stressors One-year (2014-2015) observations on the
of climate change and pollution. We will demonstrate ecosystem scale CO2/H2O fluxes and other micro-
how we could combine basic research, applied meteorological observations were analysed for
research, policy research, governance, and civil society elucidating the diurnal and seasonal pattern of carbon
under a trans disciplinary framework. We will present fluxes together with their response to biophysical and
a case of on-going research in the city of Karlsruhe, environmental parameters. Daily average minimum and
Germany which is funded by the “Future Cities” maximum air temperatures in the study area ranged
program of the German Government. Our presentation between 10.31 to 32.36 °C. Environmental factors
may motivate Indian researchers, city planners, and governing carbon flux components were found to be
policy makers to formulate similar trans disciplinary related to maximum and minimum net ecosystem
research for urban trees and forests in India cities; exchange (NEE) of carbon, GPP, and the ecosystem
where problems of urban sprawling, pollution, and respiration (Re). On daily scale, maximum absorption
climate change are acute. We believe our presentation of carbon took place on 244th day of the year (DoY)
fulfills: the objective of the “Smart Cities” program by with a NEE value of -5.51 gC m-2 day-1 while maximum
the Indian Government, and Indo-German bilateral release took place on 219thDoY with a NEE of +7.83
research focus on climate change adaptation for future gC m-2 day-1. Highest GPP was observed on 244thDoY
cities. with a value of 14.12 gC m-2day-1 while lowest of 0.21
Key words: Climate change, Air pollution, Vulnerability gC m-2 day-1 on 61thDoY.Highest Re of 11.94 gC m-
assessment, Urban forestry, Urban greening, Environmental day-1 was noticed on 218thDoY and lowest of 2.50 gC
governance, Adaptation planning m-2day-1 on 12thDoY. Clear variations in monthly NEE,
GPP and Re were observed during the study period.
SII/L-4 Higher leaf area index (LAI) and sufficient moisture
conditions resulted in higher carbon sequestration during
Climate Change and Carbon September through March. During pre-monsoon season
Sequestration Monitoring in India (April-June), the NEE was lower due to lower LAI
and higher vapour pressure deficit (VPD). During the
S.P.S. Kushwaha rainy season (July-August), the carbon sequestration
potential was highly impeded by cloudy conditions and
Indian Council of Forestry Research and Education,
the rain-accelerated Re. Annual GPP, Re, and NEE of
Dehradun-248006, Uttarakhand, INDIA, Email:
spskushwaha@gmail.com sal forest was 2916.19 gC m-2year-1, 2408.32 gC m-
,and -507.89 gC m-2year-1.
Forests play a vital role in maintaining the
atmospheric carbon balance by sequestering it into For upscaling of GPP to entire Dun Valley biome,
products like biomass, wood, and timber in addition to four remote sensing-driven models, viz., (i)
improving the soil fertility, providing a large number of temperature-greenness (TG) model, (ii) greenness-
ecosystem services, and thus, mitigating climate change radiation (GR) model, (iii) light use efficiency (LUE)
over time. However, lack of accurate and spatially- model, and (iv) satellite image-based LUE (LUERS)
explicit information on the carbon exchange components model were parameterized with EC measurements and
from diverse forest ecosystem types in India limits compared with 8-day Moderate Resolution Imaging
regional scale carbon budgeting. Remote sensing-driven Spectroradiometer (MODIS) GPP products. Our
ecosystem models are well-established tools for results showed that TG model had the highest root
estimating gross primary productivity (GPP) over large mean square error (RMSE) of 1.28 gC m-2 day-1 while
areas. The present study usedtall tower eddy covariance GR and LUERS models had moderate RMSE of 0.99
(EC) data and satellite-derived variables to estimate gC m-2 day-1and 0.98 gC m-2 day-1, respectively. The
the carbon source/sink status of the a northern Indian closest GPP estimate was obtained through LUE model
mature moist deciduous/semi-evergreen sal (Shorea with RMSE of 0.93 gC m-2 day-1.The RMSE for all
robusta) forest at Barkot in Dun Valley and upscale four models were 4 times lower than that of MODIS
the GPP over entire Valley sal forest area. GPP. Lower maximum LUE ( in MODIS GPP

Abstracts 27-30 November, 2018, NBRI, Lucknow 9

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

algorithm contributed to higher RMSE. Our study be termed as nitropriming for its enormous beneficial
highlights the need of EC data for estimation of net effects instead of nutripriming. During nitropriming
ecosystem level carbon exchange and calibration of NO3 ion in fluxed in the seed and NO, an important
satellite image-based GPP model for upscaling over signaling molecule forvarious abiotic stresses may be
large area. generated via the increased activity of nitrate reductase
Key words: Climate change, Carbon sequestration, GPP. (NR) enzyme, as was observed in nitro primed okra
seeds. Therefore it can be concluded that nitropriming
SI/L-5 is a suitable option to adopt for Climate Resilient
Agriculture in this era. This lecture will summarize the
Nitrate Seed Priming (Nitropriming) impact of nitropriming on important field crops and its
possible mechanism of action in respect to stress
vis-a-vis Climate Resilient Agriculture amelioration in various field crops.
Bandana Bose Key words: Climate change, Seed, Nitro priming, Stress
responses, Field crops.
Seed Priming Laboratory, Department of Plant Physiology,
Institute of Agricultural Sciences, Banaras Hindu University,
Varanasi-221005, INDIA, Email: bbosebhu@gmail.com
In this century we are facing the biggest threat Adaptive Capacity of Different Norway
of climate change due to increasing population, demand Spruce Provenances to Lethal Drought
for food, fodder and shelter, industrialization,
urbanization including squeezing and over exploitation Tamalika Chakraborty*, Tomasz Czajkowski and
of arable land. Climate is now changing very erratically. Andreas Bolte
Hence it is the time for Climate Resilient Agriculture
so that our planet can be ready to face the challenges Thünen Institute of Forest Ecosystems, Alfred-Möller-Str.
1, 16225 Eberswalde, Germany Email: tamalika.chakraborty@
of food security now as well as in future by maintaining
thuenen.de, tomasz.czajkowski@thuenen.de, andreas.bolte
the sustainable development via adopting various @thuenen.de
technologies. Seed, being best delivery system in
agriculture the plant scientists are engaged in Events of severe heat waves and droughts are
developing various technologies which can protect crop becoming frequent in central Europe and impacting
seeds from various stresses, faced during germination forest ecosystems. Norway spruce (Picea abies) is
in field. Seed priming is where controlled hydration and the most important commercial tree species in Germany.
dehydration is employed in seed by initiating the process However, spruce is sensitive to drought, and thus
of germination but the protrusion of radicle is restricted. maintaining forest structures with spruce will increase
Seed priming ensures proper and synchronized the risk of forest damage and loss of economic value.
germination and seedling development. Since more than Our recent studies on European beech (Fagus
three decades Bose and her group are working with sylvatica) revealed a high variation of drought
seed priming technology using various salts containing tolerance among beech provenances. This enables to
nitrates, phosphates, carbonates and sulphates and have increase drought resistance and resilience of beech
observed that among these, salts of nitrates performed forest stands by selecting more drought tolerant
best in normal as well as in stressed conditions. Plants, provenances from the rear edge of the beech forest
obtained from nitrate primed seeds develop the capacity distribution with low precipitation rates within the
to tolerate the drought, heat, cold, submergence and growing season. Motivated by our previous research
heavy metal stresses. This treatment also improves the on European beech, we studied response of Norway
quality of the produce. The influence of nitrate spruce sourced from different European provenances
treatment has been drawn on the basis of studies related to extreme drought. In a greenhouse pot experiment, a
to germination physiology, nitrogen and antioxidant lethal drought was simulated using spruce seedlings of
metabolism in the growing plants and their yield eight provenances in summer of 2017 (treatment: 100
attributes. Therefore now this treatment technology can plants per provenance, control: 20 plants per

10 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

provenance). Drought was applied until plants’ death; because the country is endowed with a variety of plants
during the experiment soil water availability (SWA, and animal species found in the boundless forests and
percentage proportion of available water AW), gas grasslands. Nigerian plant species occur in different
exchange, plant water status (pre-dawn water potential) numbers within the country’s vegetation that range from
and survival rate for each provenance were recorded. the rainforest and mangrove along the coast in the South
Based on survival rates, symmetric logistic survival to the Sahel in the North. Most of the plant species
functions (2 PL) for the different provenances were sustain the rural economy. Attention of Nigerians has
fitted. We found varying responses of mortality of been focused on plant species because of their forestry
plants between provenances to drought. The critical importance and commercial uses in a number of ways
mortality rate of 50% (LD50) at remaining soil water that affect human lives such as construction works,
availability (SWA) ranged from 7% for a provenance nutrition, entertainment, forensic medicine,
from East-German lowland with low yearly precipitation pharmaceutical and cosmetic industries. In spite of the
to 8.3% for a Bavarian mountainous provenance with socio-economic and medicinal values of the plant
considerably higher yearly precipitation. First seedlings species to human well-being and sustainable
of all provenances died when SWA had fallen below a development, Nigerian plant species are still facing
critical threshold of 20%. These outcomes demonstrate prejudicial challenges of climate change. These
the general possibility to increase drought resistance ecological dilemmas were directly attributed to high
of Norway spruce regeneration by using more drought- level of human activities due to deforestation, over
tolerant provenances. population, over harvesting of wild plants for logging,
Key words: Adaptive forest management, Climate change medicine and food; pollution in the cause of energy
impacts, Drought, LD50, Provenance trial, Tree mortality. generation through firewood, electric power supply and
industrialization processes. The consequent effects of
SI/O-2 which were decline in soil fertility, lowering of potable
water, shift in the seasons, starvation, increase in
Potential Impacts of Climate Change incidence of pests and diseases and increased crime
and Air Pollution on Plant Diversity in rate. This paper highlights the threats, challenges and
effects of climate change and air pollution on the
Nigeria: A Review Nigerian plant species as well as suggesting possible
remedy to the menace through intensive afforestation
O.M. Mudasiru1,2*, A.E. Ayodele2 and T.S. Rana1 programmes by establishing woodlots, shelterbelts,
Plant Diversity, Systematics & Herbarium Division, CSIR- orchards and agro-silvicultural system as well as the
National Botanical Research Institute, Rana Pratap Marg, application of effective environmental management
Lucknow-226001, Uttar Pradesh, INDIA; 2Department of actions to reverse the process and safe-guard future
Botany, University of Ibadan, NIGERIA Emails: occurrences.
opeyemi.mudasiru2013@gmail.com, bayodele@yahoo.com,
ranats@nbri.res.in, a.e.ayodele@mail.ui.edu.ng, ranatikam Key words: Climate change, Deforestation, Air pollution,
@gmail.com Over-harvesting, Nigeria.

Climate change has become an issue in SI/O-3

biodiversity sustainability and is one of the most
disastrous global challenges of the millennium. Climate Study of Ecotypes with Respect to
change gives rise to high temperature, erratic rainfall,
high sea level and drought. Vegetation reflects
environmental conditions. The diversity and the Nisa Yusuf Babwane
distribution of the world’s vegetation is the product of
a complex suite of interactions between individual plants, SIES college of Arts, Science and Commerce, Sion West,
animals and a multitude of climatic and environmental Mumbai- 400022, Email ID: nisababwane@gmail.com
variables. Every plant and animal plays a role in the This study dealt with physical and physiological
forest ecosystems. Nigeria is rich in biodiversity characters of plants growing in favorable and

Abstracts 27-30 November, 2018, NBRI, Lucknow 11

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

unfavorable condition. Plants selected for this study mismanagement of apple and cherry, and postharvest
were Cynodon dactylon, Nerium oleander and Ficus sprouting of onions and potatoes. Following standard
benjamina. Leaf samples collected from the plants procedures of harvesting and post harvest handling can
were studied and analysed. Physical characters were drastically reduce postharvest losses, with no or little
includes leaf area index, dust load on the leaves, injured additional cost, but certainly high returns. However,
leaf area, pH of soil and water holding capacity of soil. stakeholders, especially growers, neither know the
Physiological characters were includes protein content cause of problem nor the means to control it. So first
of leaves, starch content of leaves and chlorophyll and foremost step would be to sensitize and practically
content of leaves and also total carbon content of soil. educate them about the causes of problem, and the
The results of this study found that the plants growing need and ways to control it. Though simple and standard
in favorable region have more productivity as compared techniques do exist in developed counties to address
to the ones growing in unfavorable region. Plants many such causes, but the access/means and
growing in garden site differ from plants growing in knowledge about them is lacking among stakeholders
roadside both morphologically and physiologically. in developing world (like Kashmir). Thus, practical
Key words: Leaf area index, Dust load, Water holding education/ training of stakeholders through Postharvest
capacity, Roadside. Training and Services Centers (PHTSC) can help in
reducing Postharvest Losses, which in turn can increase
SI/O-4 food availability and food security and thereby reduce
over-reliance on production-oriented activities and
Postharvest Training and Services hence help in environmental protection.
Centers can Help in Food Security and Key words: Postharvest Losses, Training, Education,
Knowhow, Handling, Food security, Pollution.
Environmental Protection by Reducing
the Postharvest Losses SI/O-5

Mohmad Arief Zargar Impact of Climate Change on the

Department of Botany, University of Kashmir, Srinagar- Potential Distribution of Himalayan
190006, J&K, INDIA, Email: arifk11@yahoo.co.in
Juniper (Juniperus squamata), an
30-50% (1.2–2 billion tons) of all food produced Alpine Shrub in Himalayas
on the planet is lost and not consumed. 40–50% of
horticultural crops produced in developing countries are Maroof Hamid 1 *, Anzar A. Khuroo 1 , Rameez
lost before consumption. It indicates that about 50% Ahmad1 and Bipin Charles2
of investments made for production of food goes waste,
especially on fertilizers, pesticides, fuel, energy, labour Centre for Biodiversity and Taxonomy, Department of
Botany, University of Kashmir, Srinagar-190006, J&K, INDIA;
and water. Food production (agriculture) is one of the 2
Ashoka Trust for Research in Ecology and the Environment
major causes of pollution and even climate change. So (ATREE), Royal Enclave, Srirampura, Jakkur PO, Bengaluru-
rather than increasing food production, it is better to 560064, INDIA, Email: hamidmaroofmudasir@gmail.com
reduce the loss of what is produced. Therefore,
reducing postharvest food losses are more fruitful than In recent times, the rising annual mean
increasing food production in terms of economic and temperatures due to contemporary climate change
environmental costs/returns. Commodity System have shifted the latitudinal and altitudinal limits of many
Assessment (through well-structured questionnaires) species. This is particularly concerning for alpine
of fresh fruits (apple and cherry) and vegetables (onion species, for which there may not be sufficient suitable
and potato) in Kashmir revealed improper harvesting habitats available at higher altitudes to facilitate their
and obsolete practices of post harvest handling and upslope migration. Juniperus squamata (Himalayan
management as main causes for huge post harvest juniper) is a dwarf cushion-forming alpine shrub, which
losses. The biggest causes are temperature occurs at an altitude ranging from 3000-4500 m, and

12 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

acts as natural conservation microrefugia for many countries particularly India. Conservation agriculture
threatened species endemic to Himalayas. The present (CA) offers a low-cost strategy for mitigating climate
study models the potential distribution of J. squamata change by sequestering carbon in soil and is based on
using ensemble approach in Biomod2 package for the principles of minimal tillage, residue recycling and
present and future (RCP’s 2.6 and 8.5 covering 2050 crop diversification for resource conservation and
and 2070). The results indicated an expansion of sustainable agriculture promote soil carbon restoration
climatic suitability of J. squamata towards much higher by tipping the balance in favor of carbon inputs relative
elevations into areas that are currently unsuitable and to carbon outputs. Carbon storage (sequestration) in
on the other hand, several currently suitable areas may the soil can be achieved by maximizing carbon inputs
become climatically low or unsuitable in the future. and minimizing carbon outputs. Soil carbon fractions
Currently, the total climatically suitable area for this are influenced by the agronomic management practices
species is 126774.66 km2 , with an additional area adopted in CA systems. Contrasting cropping systems,
becoming suitable under RCP 2.6 (2050 and 2070) and tillage and residue management have a
RCP 8.5 (2050 and 2070). The results also revealed a significantinfluence on soil quality, C and N cycling.
northward shift and upslope advance of existing suitable SOM stratification (SR) and carbon management index
climate for J. squamata in future climate scenarios. (CMI) as an indicator of soil quality, related to the rate
Hopefully, the research insights from the present study and amount of SOC sequestration generally used for
will help in understanding the impacts of climate change natural and managed ecosystems. In general, the high
on alpine vegetation in Himalayas with wide implications values of stratification ratio (proportion of a soil
for scientifically-informed adaptation and mitigation property at the surface layer to that at a deeper layer)
strategies. indicate good soil quality and are usually used to assess
Key words: Alpine, Climate change, Himalayas, Microrefugia, agricultural practices. Experimental trials in two
Mountains. research station (Karnal, Haryana, India and
Samastipur, Bihar, India) on CA was established in
SI/O-6 2012.This study aimed to 1) assess the stratification of
soil carbon pools; and 2) evaluate the soil quality of
Impact of Conservation Agriculture different CA practices using SR and CMI values as
Practices on Productivity and the primary assessment parameters. Soil carbon stock
increased in zero tillage and permanent beds as
Stratification of Soil Carbon in Indo- compared to conventional practices. Responses of SR
Gangetic Plains of India in different CA practices to change of soil depth were
significantly different with the highest value in zero
Ajay Kumar Mishra1*, Hitoshi Shinjo1, Hanuman tillage. The SR values of labile carbon fraction differed
Sahay Jat2, Mangi Lal Jat2, Raj Kumar Jat3 and significantly only up to plow layer (0-5:5-15 cm). Short-
Shinya Funakawa1 term practices (5-years) of CA in Indo-Gangetic plains
1 of India influences the distribution and stratification of
Terrestrial Ecosystem Management Laboratory, Graduate
School of Global Environmental Studies, Kyoto University, soil carbon pool. Our study revealed that SR of carbon
Kyoto, JAPAN; 2 International Maize and Wheat pool serves as an efficient indicator for short-term
Improvement Centre, New Delhi, INDIA; 3Borlaug Institute changes offered by CA practices. CA systems not only
of South Asia, International Maize and Wheat Improvement sequestering substantial carbon in soil but also enhances
Centre, Bihar, INDIA, Email: akm8cest@gmail.com productivity and resilience of the cropping system to
Climate change and food security threatens the attain the goal of sustainable agriculture.
present agriculture and is of great concern in developing Key words: Carbon sequestration, Stratification ratio,
Infiltration rate, System productivity, Residue load.

Abstracts 27-30 November, 2018, NBRI, Lucknow 13

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SI/O-7 SI/O-8

Winged Bean (Psophocarpus Estimation of Carbon Sequestration

tetragonolobus L. DC.): A Completely Potential of Citrus sinensis in Tropical
Edible and Underutilized Legume in Zone of Arunachal Pradesh
Tropical Countries for Food and
R. Bordoloi*, H. Dabi, B. Das, A. Paul and O.P.
Nutritional Security Tripathi

Ajeet Singh* and P.C. Abhilash Department of Forestry, North Eastern Regional Institute of
Science and Technology (Deemed to be University), Nirjuli-
Institute of Environment & Sustainable Development, 791109, Arunachal Pradesh, INDIA, Email: ritashree.100@
Banaras Hindu University, Varanasi-221005, INDIA, gmail.com
Email: ajeetbhu97943@gmail.com
The study was carried out to determine the carbon
Winged bean (Psophocarpus tetragonolobus stock and net carbon sequestration potential of land
DC), aptly described by National Academy of Sciences, cover under Citrus sinensis plantation in tropical agro-
United States of America as a ‘supermarket on a stalk’ climatic zone of Arunachal Pradesh. Altogether, fifteen
is an underexploited legume of the tropics. The leaves, (0.1ha each) permanent plots were established along
shoots, flowers, young pods, seeds and tubers are all the altitudinal gradients and carbon stock was measured
edible and rich sources of proteins, minerals and using standard methodologie / allometric equations and
vitamins. The crop can be luxuriously grown in hot and carbon sequestration potential using age of the
humid tropical countries and can be easily grown on plantation crops. Soil samples were also collected in
roadside, boundaries of field, and home gardens. Owing replicate and brought to the laboratory for further
to its high nutritional significance and ecological chemical analysis. Stand density of the plantation ranges
adaptations, it can be projected as a futuristic crop from 476 stems/ha to 620 stems/ha having the basal
under changing climatic conditions. Therefore, area variation of 3.36 m2ha-1 to 10.63 m2 ha -1. The
extensive field survey has been conducted in Eastern individuals have been grouped into four age classes
Uttar Pradesh for knowing the occurrence and i.e., 10, 15, 20, >25 years. The aboveground biomass
distribution of winged bean as well for collecting values of the plantation crop were 23.45 Mg ha-1, 30.57
germplasm for large-scale exploitation. While the Mg ha-1, 70.89 Mg ha-1 and 71.43 Mg ha-1 in aforesaid
cultivation of winged bean is extremely low in Eastern age-classes. The belowground biomass was 8.54 Mg
UP, our study indicates that it can grow well in hot and ha-1, 8.86 Mg ha-1, 20.56 Mg ha-1 and 20.71 Mg ha-1 in
humid environment and even in disturbed and nutrient 10, 15, 20 and >25 year old plantation, respectively.
poor soils. Largescale exploitation of winged bean can Total biomass carbon calculated from the plantation
complement the food and nutritional security initiatives crop ranged between 20.89 Mg C ha-1 and 50.67 Mg
in tropical countries and therefore, suitable conservation C ha-1 and biomass carbon accumulation was positively
and crop improvement programs are imperative for correlated and increased with increase in age of
ensuring the environmental sustainability of winged plantation (r2 =0.99). The soil organic carbon content
bean cultivation. ranged from 35.89 Mg C ha-1 to 75.76 Mg C ha-1
Key words: Environmental sustainability, Food security, among plantation the different ages. The litter carbon
Nutritional security, Tropical countries, Winged bean. content varied from 0.56 MgC ha-1 to 2.46 Mg C ha-1.
Total carbon stock in the present study ranges from
57.79 Mg C ha-1 to 101.44 Mg C ha-1. It was found
that older plants sequester comparatively more carbon
than the younger ones except in some of the carbon
pools like litter and soil, which could be mainly attributed
to management practices in form of litter collection.
The combined approach of statistical regression along

14 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

with field based data were used to predict the carbon SI/O-10
density map using various geospatial tools coupled with
satellite data. Detection of Mycoflora and Mycotoxin
Key words: Carbon sequestration, Citrus plantation, in Raw and Roasted Peanut Kernels
Regression, Vegetation indices. in Bangladesh
Md. Maniruzzaman Khandaker 1 *, Tasmilur
Integrated Farming Strategies for Rahman1, Motiur Rahim2, Maksuda Begum2 and
Tariqul Islam2
Climatic Resilient Agriculture under 1
Department of Botany, Jagannath University, Dhaka,
Rain-Fed Conditions in North West BANGLADESH; 2Food Toxicology Research Lab, BCSIR,
Himalayan Regions Dhaka, BANGLADESH, Email: maniruzzamanbot@gmail.com
Raw peanut kernel samples were collected from
Gulshan Kumar* and Hem Chander thirteen areas of Bangladesh for determination of
Division Botany, Department of Bio-Sciences, Career Point mycoflora and mycotoxin. Fungi associated with the
University Hamirpur, Himachal Pradesh-176041, INDIA tested samples throughout the investigation were
Email: sharmagulshan1980@gmail.com, hemchander78@ Aspergillus flavus, A. niger, Aspergillus sp. (1),
gmail.com Aspergillus sp. (2), Penicillium sp., Fusarium sp.,
The results of the national innovation on climatic Rhizopus sp. and Curvularia sp. A. flavus was the
resilient agriculture (NICRA) investigations on most occurred fungus followed by A. niger. Colonies
integrated farming strategies to cope with rain-fed of fungi were formed in 79.81% to 98.10% of raw
conditions of Hamirpur district of Himachal Pradesh peanut kernels. Out of the thirteen samples, eight
are being described. On the basis of hydrological and samples were found to be contaminated with the range
technical as well as social and cultural conditions, 11.91ppb to 182.62ppb of total aflatoxins and five
appropriate technological strategies have been samples were free from aflatoxins. Another ten
implemented to combat the effects of climate change. samples of roasted peanut kernels were collected from
The main objective was to enhance the resilience in different areas of Bangladesh for determination of
agriculture. The top most priority of the present study mycoflora and mycotoxin association. Fungi associated
was to way out the source of water for meeting with the roasted samples were Aspergillus flavus,
minimum critical water needs of crop diversification Aspergillus niger, Aspergillus sp. (1), Aspergillus sp.
and maintenance of ecological balance under rain-fed (2), Penicillium sp., Fusarium sp., Rhizopus sp. and
conditions and to analyze their technical/economical Curvularia sp. A total of 3630 fungal colonies were
feasibility as well as their affordability for future users. formed. Among these fungal colonies the highest
The present study reveals that it is economically feasible 39.12% of colonies were formed by Aspergillus flavus
to apply decentralized technologies and strategies in and the lowest 1.68% colonies were formed by
farming system for climatic resilient agriculture Curvularia sp. The highest 73.91% of the kernels of
universally. sample 01 were found infected while in sample 05 it
was 25.17%. Out of ten roasted peanut samples four
Key words: Crop diversification, Livestock, NICRA, Rain- were detected with various amounts of aflatoxins (B1,
fed, Resilience. B2, G1, G2). The highest 38.94 ppb of total aflatoxins
were detected in sample 02 and the lowest 5.67 ppb of
total aflatoxins were found in sample 01. Six roasted
samples were free from aflatoxins.
Key words: Raw peanut kernel, Roasted peanut kernel,
Mycoflora, Aspergillus flavus, Aflatoxin.

Abstracts 27-30 November, 2018, NBRI, Lucknow 15

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SI/O-11 Overall a complex interlinking was found between

microclimatic variables, photosynthetic responses and
Overstory Species Regulation of species association. Polynomial quadratic regression
Understory Tree Physiology and functions applied to explore relationships, showed PAR
and vapour pressure deficit (VPD) as major factors
Microclimate: A Case Study in Three limiting understory leaf level photosynthesis. Addressed
Contrasting Tropical Deciduous Forest diurnal and spatial variability in microclimate, tree
Associations of Terai Region in physiology along different species associations will
provide vital inputs for various dynamic global
Northern India vegetation models in predicting and refining net
ecosystem exchange at regional and global level.
Nayan Sahu
Key words: Understory photosynthesis, Gas exchange, Plant
Department of Botany, Indira Gandhi National Tribal functional types, Sunflecks, Leaf area index, Ecophysiology.
University, Amarkantak, M.P., India, Email: nayansahu@
igntu.ac.in SI/O-12
The understory is imperative to tropical forest
ecosystems and reflects a high stress environment Mid-Pliocene and the Present Climate
where vital resources that plants need are often in short -Vegetation Equilibrium: Arctic-Asian
supply. Understory lacks much needed detail
physiological data for the development of site and
Monsoon Teleconnections
species-specific predictive carbon budgeting models of
Anjum Farooqui* and Salman Khan
understory vegetation response to change in forest types
and management strategies. Exhaustive leaf gas Birbal Sahni Institute of Palaeobotany, 53 University Road,
exchange, chlorophyll fluorescence, microclimate, leaf Lucknow-226007, INDIA Email: afarooqui_2000@yahoo.com
water relations, Leaf area index (LAI) and The sea ice covers in the Arctic contributes to
Phytosociological attributes were quantified across seasonal weather changes in tropics and have immense
three contrasting forest association (FA): Dry mixed implications on Earth’s climate. The oceanic circulation
(DM), Teak forest, and Sal mixed (SM) in tropical moist leading to exchange of cold and warm water creates
deciduous forest of terai region for investigating role weather conditions that we experience. During Mid-
of over story tree association in controlling microclimate Late Pliocene epoch the CO2 level reached 355-415ppm
and understory tree physiological performance. The and the temperature was 2-3oC higher than the present.
variations were largely explored amongfive understory Global climate is rapidly evolving in present time with
woody species (Mallotus philippensis, Tectona increased CO2 concentration (406 ppm: NOAA, 2017)
grandis, Shorea robusta, Clerodendrum viscosum and the projected global mean temperature is estimated
and Tiliacora acuminata belonging to four different to be 1.84-3.60°C. An alarming rate of CO2 increase
growth forms (understory tree, tree sapling, shrub and is attributed to global warming enhanced by
climber). Dominant monoculture Teak FA showed anthropogenic activities. Since vegetation of a particular
higher diurnal photosynthetic rates (all the 5 species) area at a given time is in equilibrium with the prevailing
owing to increased understory PAR (Photosynthetically climatic conditions, the pollen grains preserved in the
active radiation) availability (due to applied silvicultural natural archives are derivatives of the then existing
practices and uniform phenophases). Heterogeneous vegetative cover in a particular time bracket. The
canopies (SM) provided extremely low PAR and sun vegetational reconstruction through pollen evidences
fleck (availability) to understory vegetation thereby in sediments allows us to infer climatic conditions and
limiting assimilation rates as compared to Teak forest its fluctuation through a time period. The palynological
under ambient conditions. Dry mixed FA showed higher studies of IODPHole (IODP-910C) from Arctic region
understory LAI values in contrast to other FA due to has been partially analyzed in a time bracket of 3.4-2.6
modified local competitive hierarchies, heterogeneous Ma (Mid-Pliocene Epoch). The overall palynological
resource availability and utilization among species. results reveal a high percentage of broad leaf temperate

16 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

taxa namely Alnus, Corylus, Betula, Carpinus, difference (p<0.05). On an average, the yield of maize,
Quercus suggesting a relatively warmer temperate turmeric and ginger intercropped were 2.68, 6.15 and
climate in the region supporting the vegetation that 6.96 t ha-1 respectively, which are lower than average
grows well in frost free climatic conditions. Similar yields from sole crops. It was observed that the
synchronous trend of high to low percentage of intercropping of maize had significantly (p<0.05)
pteridophytic and bryophytic spores along with fungal increased yield while turmeric and ginger decreased
spores was observed. The study is also supported by over the years. Maximum above ground biomass (33.35
moderate percentage of dinoflagellate cysts, poorly Mg ha-1) and carbon storage (15.67 Mg C ha-1) was
preserved brackish water centric diatoms indicating the found in Oil palm +Maize intercropping systems with
paralic sediment deposition. The inferred warm significantly higher carbon sequestration rate (5.22 Mg
temperate climate in the Arctic polar region during the C ha-1 yr-1) as compared to other treatments. The results
mid-Pliocene and its consequences on the Asian indicate that intercropping in juvenile oil palm plantations
Monsoon system is potentially important for predicting could have coupling effects both beneficial in providing
the impacts of global warming in the near future. additional crop yield and also carbon sequestration
Key words: Pliocene, Climate, Vegetation, Arctic, India. services. The practice can be an alternative to shifting
cultivation and helpful in reclaiming degraded lands in
SI/O-13 the wake of climate change mitigation efforts.
Key words: Intercropping, Oil palm, Carbon sequestration.
Intercropping Practices in Juvenile Oil
Palm Plantations to Evaluate Crop SI/O-14
Yield and Carbon Sequestration Prospects of Bamboo Resources
Services in Mizoram, Northeast India Available in Northeast India in Boosting
Soibam Lanabir Singh, Uttam Kumar Sahoo* and Socio-Economic Condition of the
Anudip Gogoi People and in Mitigating Climate
Department of Forestry, School of Earth Sciences and Natural Change
Resource Management, Mizoram University, Aizawl-796004,
Mizoram, INDIA, Email: uksahoo_2003@rediffmail.com; L.B. Singha1*, O.P. Tripathi1, M.L. Khan2 and R.S.
uttams64@gmail.com Tripathi3
Oil palm cultivation involves a long gestation period 1
Department of Forestry, North Eastern Regional Institute
(3-4 years) in the initial years after planting posing a of Science and Technology (Deemed to be University),
threat to smallholder farmers, and hence intercropping Nirjuli, Arunachal Pradesh, INDIA; 2Department of Botany,
could provide for additional revenue. The experiment Dr. H.S. Gaur Central University, Sagar, Madhya Pradesh,
was carried out between 2016 and 2018 to determine INDIA; 3National Botanical Research Institute, Lucknow-
the yield of crops and its effect on growth and 226001, UP, INDIA Email: lbsingha@hotmail.com,
development of main oil palm. Different intercropping khanml61@gmail.com, tripathirs@yahoo.co.uk
experiments were designed with standard oil palm Northeast India is rich in bamboo resources as it
density (148 palms ha -1 ) in an intensive three-year provides favorable ecological niches for different
sequential cropping. Complete randomized block design bamboo species. The region harbors more than 60%
with three replicates was adopted and the treatments of the country’s bamboo resource in terms of diversity,
consisted of: Sole oil palm (T0); Oil palm + Maize (T1); and more than 66% of the growing stock of bamboos
Oil palm + Turmeric (T2); and Oil palm +Ginger (T4). is present in this region. They are mostly woody, native
Results showed that maximum oil palm growth and endemic. Only a few exotic species are found in
development in terms of height, girth and estimated the region. Bambusa balcooa, B. tulda, B. nutans,
above ground biomass was observed in T0, however, B. cacharensis, B. polymorpha, Dendrocalamus
the different treatments did not have any significant giganteus, D. hookeri, D. sikkimensis, D. latiflorus,

Abstracts 27-30 November, 2018, NBRI, Lucknow 17

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Schizostachyum dullooa, Melocanna baccifera etc. the main determinant of the ecosystem C-balance.
are large-sized bamboos with high commercial values Changes in soil organic carbon storage as well as in
and multiple applications. Tender shoots ofca.16 soil respiration may affect the atmospheric CO 2
bamboo species in both fresh and fermented forms are concentration and global carbon cycle. Thus the present
highly commercial as vegetable with an annual study was undertaken to find the affects of
consumption of more than 7000 tonnes and a net annual environmental parameters on soil respiration rates
income of ca. 50 million rupees, contributing under Pinus roxburghii vegetation cover using
significantly to the socio-economic condition of the rural environmental gas monitor instrument. CO2 emissions,
people. Most of the bamboo species occurring in the soil temperature and soil moisture were recorded on
region are grown in home gardens, whereas few monthly basis. Results of the study reveal that the rate
develop into pure brakes forming secondary of soil respiration increased with increase in
successional forests. Due to fast growing nature, temperature and soil moisture. Thus, the soil
bamboos act as an important carbon sink. The rate of temperature and soil moisture content exerted the
carbon sequestration by bamboos may exceed that of primary and secondary factors to control soil respiration
the native tree species. Large-sized bamboo species respectively. Maximum soil respiration rates were
accumulate more than 290 tonnesha-1 biomass with a observed during June (0.34 g CO2 m-2 hr-1) and July
net primary productivity of ca. 47 tonnes ha-1 yr-1, which (0.75 g CO2 m-2 hr-1). These rates may be attributed to
is almost double the rate exhibited by eucalypt clones. the high soil temperature and soil moisture rates during
The carbon pool in standing crop of many bamboo these months besides higher air temperature and
species with ca. 149 tonnes of C ha-1 in 6 years old precipitation factors which start declining from August.
plantations is even higher than that of 40 years old teak Seasonally, soil respiration showed positive relationship
forests which accumulate hardly ca. 126 tonnes of C with soil temperature and soil moisture. Therefore, soil
ha -1 . respiration rates can be affected by climate change.
Key words: Bamboo resources, Northeast India, Carbon Key words: Soil respiration (Rs), Soil Temperature (Ts), Soil
sequestration, Carbon pool, Standing crop Moisture (SM).

SI/O-15 SI/O-16

Soil Temperature and Soil Moisture Ecosystem Carbon Pool and Net
Influence on Soil Respiration Rates Primary Production in a Tropical
under Pinus roxburghii Vegetation Matured Forest of North-Eastern
Cover India
S. Sivaranjani* and V.P. Panwar Ratul Baishya1* and Saroj Kanta Barik2
Forest Soils Discipline, Forest Ecology and Climate Change Department of Botany, Faculty of Science, University of
Division, FRI, Dehradun, INDIA Email: ranjani.agri@gmail. Delhi, Delhi-110007, INDIA; 2National Botanical Research
com, vppanwar@yahoo.com Institute, Rana Pratap Marg, Lucknow-226001, INDIA, Email:
rbaishyadu@gmail.com, sarojkbarik@gmail.com
The amount of soil respiration that occurs in an
ecosystem is controlled by several factors. Carbon Tropical forests of north-east India provide such
enters in terrestrial ecosystem through photosynthesis vital ecosystem services as biomass and carbon
and return back into atmosphere as soil respiration. In sequestration. However, sufficient data on all these
forest ecosystem, respiration is mainly dominated by aspects are limited and mostly aboveground biomass
soil respiration which accounts for about 60-80 per cent information is only available. Most biomass estimation
of the total respiration. The soil respiration has been studies involving Net Primary Production (NPP)
reported to account for carbon emission of >100 Pg estimation considers only the increment in aboveground
yr-1 globally and specifically in forests, may represent biomass (AGB) and litter fall, completely ignoring the

18 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

belowground biomass (BGB) component. For number of hours of sunshine in the month of November-
estimating ecosystem level NPP of a forest, time series December.
biomass data for tree, shrub, herb and litter components Key words: Dendro-climatology, Palaeoenvironment,
are pre-requisites. This data gap prompted us to Conifers, Tree-rings, Climate.
undertake this study in the tropical matured forests of
Meghalaya, North-Eastern India. We attempted to SI/O-18
study the species diversity, ecosystem biomass,
ecosystem carbon pool including aboveground, Understanding Interaction of Abiotic
belowground component, soil carbon, detrital biomass Variables on Net Primary Productivity
and NPP of the forest. The forest biomass was
estimated using aboveground and belowground in Indian Tropical Deciduous Forest
allometric models. The aboveground biomass,
belowground biomass, herb and shrub biomass and Soumit K. Behera1,2*, M.D. Behera2 and R. Tuli1,3
detrital biomass were 313.8 Mg ha–1, 50.82 Mg ha–1, 1
Plant Ecology and Climate Change Science Division, CSIR-
12.43 Kg ha –1 , 37.11 Kg ha –1 and 9.84 Mg ha –1 , National Botanical Research Institute, Lucknow-226001,
respectively. The tree diameter classes between >30- INDIA; 2Centre for Oceans, Rivers, Atmosphere and Land
80 cm girth classes contributed 202.75 Mg ha–1 and Sciences, Indian Institute of Technology, Kharagpur, INDIA;
31.56 Mg ha–1 contributing 64.61% and 62.1% to the UIET, Panjab University, Sector 25, Chandigarh, INDIA,
Email: soumitkbehera@gmail.com
total tree AGB and BGB. The total soil organic carbon
upto 1 m depth was 83.22 Mg C ha–1. The ecosystem Net primary productivity (NPP) represents a
biomass, ecosystem carbon, total aboveground NPP major component of net ecosystem production (NEP),
and belowground NPP in the forest were 374.51 Mg which is the net carbon (C) input from the atmosphere
ha–1, 265.52 Mg C ha–1, 12.26 Mg ha–1 yr-1 and 1.35 into the biosphere, the other component being the
Mg ha–1 yr-1, respectively. The tropical matured forest release of C through decomposition or combustion of
of the region showed higher carbon sequestration organic matter. NPP is considered to be the best
capacity in comparison to other forests. integrator measure of resource effects on ecosystem
Key words: Ecosystem biomass, Net primary production, processes. Patterns of terrestrial NPP may change in
Tropical matured forest, Carbon sequestration. response to changes in species association,
microclimate, CO2 concentration, natural disturbances
SI/O-17 and other environmental factors. Improved
understanding of plant functional types (PFT) level NPP
Tree Ring Analysis of Pinus roxbhu- may aid predictions of ecosystem response to ongoing
rghii from FRI, Dehradun climate and land-use changes. Modelling NPP in
tropical deciduous forests is very challenging and suffers
Sangeeta Gupta and Tarun* from data inadequacy and field measurements. Field
data on the interactions of tropical forest NPP to abiotic
Forest Research Institute, New Forest, Dehradun, factors are needed to resolve uncertainties among PFTs
Uttrakhand-248006, India, Email: tarunfri@gmail.com
particularly for seasonally dynamic deciduous
The study presents the dendro-climatic response ecosystems. Investigating the NPP patterns among
of Pinus roxbhurghii established in reserve forest of PFTs will help in assessment of carbon sequestering
Forest Research Institute estate. A collection of 3 tree- potential of these forests and further setting benchmarks
ring chronologies were studied for their dendro-climatic for carbon offset agreements among tropical countries.
response to changing temperature and precipitation. The present work investigates on the NPP patterns of
The various parameters like ring width, early wood, three PFTs {dry mixed (DM), sal mixed (SM) and teak
latewood were used to reconstruct the climatic variable. plantation (TP)} having distinct tree associations,
The tree ring chronology has a significant response to canopy stratification, different carbon assimilation rates
the minimum temperature of May-June. The ring and microclimate within a broad Indian tropical
chronology also had a significant correlation with the deciduous forest. We also tried to analyse the

Abstracts 27-30 November, 2018, NBRI, Lucknow 19

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

biodiversity (represented by species richness, SR) and production and litter quality during exposure to e-CO2
productivity (NPP) interactions pattern emerging in which could significantly alter the input of nitrogen,
above three PFTs. Principal component analysis soluble phenolics, soluble sugars, lipids, cellulose, and
(PCA) and generalized linear models (GLM) were used lignin to soils, and so also the biogeochemical cycle of
to examine the relationship between individual the prevailing ecosystem.
structural, taxonomic and microclimatic variables Key words: FACE, Tropical species, Litter functional trait,
against NPP within individual PFTs. GLM analysis Mass loss, Nutrient flux.
revealed that leaf area index (LAI) as the best predictor
for NPP followed by air temperature among SI/O-20
microclimatic variables in all 3 PFTs.
Key words: Tropical deciduous forest, Leaf area index, Net Tree Community Regulate Soil
primary productivity, Plant functional types, Generalized Multifunctionality in a Tropical Dry
linear models.
Ashutosh Kumar Singh1,2* and Nandita Singh2
Litter Functional Traits and Nutrient 1
Academy of Scientific and Innovative Research (AcSIR),
Dynamics in Tropical Trees Species CSIR-National Botanical Research Institute, Rana Pratap
Marg, Lucknow-226001, INDIA; 2Plant Ecology and
under Elevated CO2 Environment in Environmental Science Division, CSIR-National Botanical
India Research Institute, Lucknow-226001, INDIA, Email:
Apurva Rai1,2*, Ashutosh Kumar Singh1, Nandita Tropical dry forest covers large areas of tropical
Singh1 and Nandita Ghoshal2 region and characterized by a mosaic type of tree
Plant Ecology and Environmental Science Division, CSIR- communities that can be recognized as a co-dominant
National Botanical Research Institute, Lucknow-226001, multi-species sites to predominant mono-species. The
India; 2Department of Botany, Banaras Hindu University, previous forest management practices resulted in
Varanasi-221005, INDIA Email: apurvaraievs09@gmail.com, incorporation of some exotic species and recombination
ashutosh.evst@gmail.com, nanditasingh8@yahoo.co.in, of the forest vegetation. However, we know little about
n_ghoshal@yahoo.co.in the effect of multi-specific, mono- (here Shorea
Increasing CO2 potentially leads to changes in litter robusta, Hardwickia binate and Tectona grandis)
quality and litter input in forest soils. It is, therefore and an alien-specific (Lantana camara L.) sites on
vital to understand leaf litter traits in response to soil multifunctionality (provision of multiple ecosystem
increased atmospheric CO2. We conducted a field functions and services together that linked to soil). We
experiment to examine how the litter functional traits were assessed soil individual functions such as
and nutrient dynamics respond to e-CO2 concentration availability of soil nutrients (N and P), fractions of soil
in FACE (Free Air CO2 Enrichment) facility in CSIR- organic carbon (SOC) and glomalin related soil protein
NBRI, Lucknow, U.P, India. Litter was analysed for (GRSP), microbial biomass C, CO 2 efflux and
concentrations of carbon (C), nitrogen (N), soluble extracellular hydrolytic (acid and alkaline phosphatase,
sugars (SS), lipids, lignin, cellulose, hemi-cellulose (HC) â-glucosidase, dehydrogenase and fluorescein
and soluble phenolics (SP). We found that elevated diacetate) and oxidative (phenol oxidase and
CO2 significantly increased C/N, lowered litter N peroxidase) enzymes to topsoil (0-15 cm depth) and
concentration in leaf litter of Butea and Teak plantations, subsoil (15-30 cm depth) layers on the seasonal basis
respectively. Increase in litter biomass production (summer, winter, and rainy) in a highland TDF of India.
(Butea and Teak) under e-CO2, resulted in significant Soil multifunctionality index was calculated by averaging
increase in the flux of N, SS, SP and lipid into the soil. Z-scores of these parameters. We found that
Our study demonstrated the changes in litter biomass irrespective of soil depth and season, the extent of a

20 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

numerous soil individual functions and soil ecosystem services of tropical deciduous forests in
multifunctionality was higher at the multi-specific sites Northeast India, in general, and Kamrup district of
followed by mono- and alien-specific sites. It suggests Assam in particular. Hence, assessment of ecosystem
that under similar soil type and climatic condition higher services is extremely important to evaluate the impact
plant diversity promote soil multifunctionality. However, of ecosystem services on the livelihood of the local
in particular, we found that soil pH, alkaline phosphatase people.. Keeping above in account, present study aims
and phenol oxidase activity were higher at the alien- to assess the prevalent ecosystem services of the study
specific sites compared with native mono and multi area and examine peoples’ perceptions, knowledge and
specific sites. The multivariate (principal component attitude towards various services. Dominant services
analysis) analysis also showed the discrimination of alien related to utility were identified, categorised and ranked
specific sites from intermixed mono and multi specific following the standard methodologies. Drivers for
sites. These results suggest that L. camara sites alter change of ecosystem services were also identified,
the soil environment as they are very different from prioritized and analysed with emphasis on its impact
those created by native species. We concluded that L. on livelihood. A checklist of the plant diversity from
camara sites are least important in regards to promote the study site was recorded. Information about the
soil multifunctionality. Eventually, this study has broad condition of cultural services in terms of spiritual belief
implication in understanding revegetation of tropical was documented. Assessment of ecosystem services
forest aimed to expedite maximum ecosystem benefits. potential of selected forests was carried out with an
Key words: Glomalin related soil protein, Soil organic carbon, aim to strengthen the ongoing conservation efforts
Lantana camara, Soil enzyme, Hydrolytic enzymes, undertaken by the government, NGOs and local
Oxidative enzymes. community. Findings of the assessment would be helpful
for the decision makers to prioritise the forests for
SI/O-21 conservation purpose based on their ecosystem
Ecosystem Services Assessment and Key words: Ecosystem services, Peoples’ perception, Drivers
Community Role in Conserving the of change in ecosystem services, Dominance, Conservation.
Services in Tropical Deciduous Forests SI/O-22
of Kamrup District of Assam in North-
East India Mainstreaming the Tropical
Underutilized Legume Winged Bean
Sangeeta Deka*, Om Prakash Tripathi and L.B. [Psophocarpus tetragonolobus (L.)
DC.] for Providing Household Food
Department of Forestry, North Eastern Regional Institute of
Science and Technology, (Deemed to be University), Nirjuli- and Nutrition Security
791109, Arunachal Pradesh, INDIA Email:
deka_sangeeta@yahoo.co.in, tripathiom7@gmail.com, Vinayak Singh, Arpit Chauhan, Shafquat Fakhrah,
lbsingha@hotmail.com Sagar Prasad Nayak, Kunwar Sarvendra and
Ecosystem services accentuate on the benefits Chandra Sekhar Mohanty*
that people derive from varied ecosystems (MA, Genetics and Plant Molecular Biology Division, CSIR-
2005a). These are mainly categorized into provisioning, National Botanical Research Institute, Lucknow-226001,
regulating, supporting and cultural services. Varied Uttar Pradesh, INDIA, Email: visiranj@gmail.com,
dimensions of human well-being namely basic human arpit12191@gmail.com, shafquatfakhrah@gmail.com,
needs, economic needs, environmental needs and sarvendrakr88@gmail.com, sagar.nayak51@gmail.com,
subjective happiness are a few particular benefits
derived from the ecosystems. Literature review Winged bean Psophocarpus tetragonolobus
revealed that there is paucity of empirical data on (L.) DC. is a tropical legume with high protein and oil

Abstracts 27-30 November, 2018, NBRI, Lucknow 21

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

content. The plant is endemic to northeast India and is hunger, malnutrition, under nourishment, lack of
infested with the anti-nutrient condensed tannin (CT). adequate vitamins and minerals in their diet. Malnutrition
Leaf being the site of biosynthesis of condensed tannin among the children is rampant resulting anaemia a very
so, a comparative transcriptome analysis detected the serious problem throughout the world. Although, India
responsible transcripts and their corresponding enzymes has passed through various revolutions and achieved
in the biosynthesis of CT. Based on the similarity unprecedented development in the food crop
searches against gene ontology (GO) and Kyoto productivity and many crops have increased their
encyclopedia of genes and genomes database (KEGG) manifold productivity but the challenges of malnutrition
revealed 5210 transcripts involved in 229 different and threat of climate change continues vigorously.
pathways. A total of 1235 contigs were found to be Underutilized wild edible plants may sustain the effect
differentially expressed between high and low-CT lines of climate change; appear to be the crop of future and
of winged bean. There were 2237 simple sequence need focused attention as it can meet nutritional needs.
repeats (SSRs) in high-CT lines and 1618 SSRs in low- Present paper highlights some of the underutilized wild
CT lines. This study and its finding will be helpful in edible plants species like Basella alba, Bauhinia
providing information for functional and comparative purpurea, Boerahavia diffusa, Capparis zeylanica,
genomic analyses of condensed tannin biosynthesis in Crotalaria juncea, Diplazium esculentum, Ipomoea
legumes in general and winged bean in specific. A aquatica, Physalis minima, Portulaca oleracea,
detailed investigation on CT-biosynthesis is reported Alangium salvifolium, Anthocephalus chinensis,
for the first time in any underutilized legume crop Antidesma ghaesembilla, Averrhoa carambola,
through this study. Marker-assisted selection and Bridelia squamosa, Buchanania lanzan, Carissa
breeding between the diverse lines for mapping opaca, Cordia dichotoma, Dillenia pentagyna,
population development is underway. Validation of Diospyros exculpta, Ficus racemosa, F.virens,
specific genes and their further silencing through virus- Flacourtia indica, F.jangomas, Grewia hirsuta,
induced gene silencing is attempted with preliminary- Limonia acidissima, Madhuca longifolia,
level level of success. Regeneration of the plant from Manilkara hexandra, Pueraria tuberosa, Rumex
cotyledonary and nodal explants have successfully dentatus, Schliechera oleosa, Spondias pinnata and
optimized with an aim for establishing molecular Xeromphis uliginosa etc. as a future food crop provide
transformation of P. tetragonolobus. newer tastes, texture, aroma, flavour and recipe may
Key words: Winged bean, Anti-nutrient, Condensed tannin, sustain the effect of climate change for their sustainable
Transcriptome, Underutilized legume, Virus-induced gene utilization in commerce and trades need urgent attention
silencing. in human welfare.
Key words: Underutilized wild edible plants, Climate change,
SI/O-23 Future food plants, Sustainable utilization, Uttar Pradesh.

Underutilized Wild Edible Plants SI/O-24

Sustain Climate Change as Future
Effect of Training Systems on Light
Food Plants in Uttar Pradesh for Their
Interception and Gas Exchange
Sustainable Utilization in Commerce
Parameters in Jamun [Syzygium cumini
Trade and Market
(L.) Skeels]
Anand Prakash
A.K. Trivedi*, A.K. Singh, A. Bajpai and K.K.
Ethnobotany and Ecology Division, CSIR-National Botanical Mishra
Research Institute, Lucknow-2206001, India Email: pranand
1964@gmail.com ICAR-Central Institute for Subtropical Horticulture,
Rehmankhera, P.O.-Kakori, Lucknow-226101, INDIA
Large numbers of people in many developing and Email: ajayakumartrivedi@gmail.com
developed countries of the world are seriously facing

22 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Availability and capture of light by the canopy, SI/P-1

utilization of light in the photosynthesis and translocation
of photosynthates to the developing crop decides the Growth and Yield Responses of Wheat
productivity. Several factors individually as well as in (Triticum aestivium) Plants Treated
combination affect different steps of a physiological
process and finally quantum and quality of produce. with Ethylene Diurea (EDU) under
Orchards are usually characterized by light resource Elevated CO2
limitations. Canopy architecture and leaf display traits
affect light capture at the canopy as well as Surabhi1,2*, Veena Pandeb and Vivek Pandey1
microenvironment of each leaf. Arrangement of leaves 1
Plant Ecology and Climate Change Science, CSIR-National
as well as light capture efficiency of plant is determined Botanical Research Institute, Rana Pratap Marg, Lucknow-
by the architecture of tree canopy. Plants can maximize 226001, INDIA; 2Department of Biotechnology, Kumaun
canopy light interception by increasing both leaf surface University, Bhimtal-263136, Uttrakhand, INDIA
area and the efficiency of light interception for each Email: surabhi447@gmail.com
unit of leaf area. Manipulation of tree canopy affects Wheat is the most important food resource on
light interception, growth and productivity. Jamun the earth and is consumed by large partof the world’s
canopy exhibits lot of variation in size, compactness, population. Rising carbon dioxide and phototoxico zone
shape and branch orientation. This directly or indirectly due to anthropogenic activities in atmosphere cause
affects light interception and gas exchange parameters global warming and impact crop productivity. Ethylene
of the crop. Proper understanding of the jamun canopy diurea (EDU), a synthetic chemical, is an ozone
characteristics and gas exchange parameters may be protectant which protects plants from ozone damage.
used for new technological interventions for adequate Studies related to interactions involving EDU and CO2
light management to enhance efficiency. Variability in in wheat plants are scarce and unclear, therefore there
light interception, leaf area index and gas exchange is need to study crop response under these conditions.
parameters in jamun [Syzygium cumini (L.) Skeels] Our study evaluated the cumulative effect of elevated
cultivar CISH J-37 (Jamwant) plants grown in three CO2 and EDU on two wheat cultivars PBW154 and
systems of canopy viz., control, open and palmate were WH1105. The experiment was conducted in Free Air
recorded. Availability of diffused and direct light varied Concentration Enrichment (FACE) facility having 3
significantly viz., 27.64%, 33.38%, 29.66% and 23.33%, ambient CO2 (400ppm) and 3 elevated CO2 (550ppm)
28.67%, 25.59% in control, open and palmate canopy rings of 10 m diameter each. Each ring has 4 subplots
respectively. Considerable variation in gas exchange of 4.5 m² for each cultivar. EDU foliar spray at (200
parameters viz., net assimilation rate (5.62 - 11.78 µmol ppm) was given manually by hand sprayer on leaves.
CO2.m-2s-1), stomatal conductance (43-81 mol H2O m- Total plant height, biomass and photosynthesis of wheat
2 -1
s ), vapour pressure deficit (1.08-3.76 kPa) and cultivars showed significant differences in treated
transpiration rate (1.22-2.28 m mol H2O m-2s-1) was conditions (EDU+ECO 2 ) as compared to their
found. In accordance with this substantial variability in respective control (EDU+ACO2). We found that yield
yield and fruit quality traits was recorded. Study parameters changes e.g., grain numbers per plant,1000
exemplifies that open canopy architecture having grain weights were much more in (ECO2+EDU) as
optimal ratio of diffused and direct light, more net compared to (ACO2 +EDU) conditions. Improved
assimilation rate and stomatal conductance have better photosynthesis and better yield might be the result of
and yield and fruit quality. reduced ozone induced injury under the cumulative
Key words: Jamun, Canopy, Light interception, Leaf area treatment of ECO2 and EDU. This study showed that
index, Photosynthesis. beneficial effects of rising CO2 on C3 crops would be
partially negated by high ambient O3 concentrations.
Key words: Elevated CO2, Ozone stress, EDU (Ethylene
diurea), Wheat, Vegetative phase, Flowering phase.

Abstracts 27-30 November, 2018, NBRI, Lucknow 23

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SI/P-2 SI/P-3

Over-expression of CarMT Gene Effects of Drought on Seedling

Provides Tolerance against Drought Growth and Activity of Antioxidative
Stress by Modulating Antioxidants and Enzymes in Rice (Oryza sativa L.) with
Physiological Performances in Response to Salicylic Acid
Arabidopsis thaliana L.
Preeti Verma, Anjali Srivastava and P.K. Singh*
1,3 2
Arvind Kumar Dubey *, Rekha Sharma , Veena Department of Botany, Udai Pratap Autonomous College,
Pande2 and Indraneel Sanyal1 Varanasi-221002, INDIA, Email: drpksinghupc@gmail.com
Molecular Biology and Biotechnology Division, CSIR- Drought is one of the serious abiotic threats to
National Botanical Research Institute, Lucknow-226001, the agriculture worldwide. This is due to uneven
INDIA; 2Department of Botany, LBS PG College, Gonda distribution of rainfall and climatic conditions. Rice is
Department of Biotechnology, Kumaun University, Bhimtal one of the important staple diet severely affected by
Campus, Nainital, India Email: arvindbiotech28@ gmail.com drought induced change in plants, which results into
Drought is one of the major abiotic stress which reduction in the plant productivity. An experiment was
negatively affects plant growth and crop yield. conducted to investigate the amelioration properties of
Metallothionein (MTs) is a low molecular weight salicylic acid (SA) in rice plant under water scarcity.
protein, mainly involved in metal homeostasis, while, in Salicilic acid (SA) is recognised as signal transducing
drought stress, is still to be largely explored. The present molecule as well as regulator of oxidative damage in
study was anticipated to investigate the role of MT plants under adverse environmental conditions.
gene against drought stress. The chickpea MT based Presoaking seed treatment with 0.5mM SA was
on its up-regulation under drought stress was observed in rice plants subjected to drought condition
overexpressed in Arabidopsis thaliana to explore its in rice field. SA increased growth characteristics under
role in mitigation of drought stress. The total transcript both drought condition and irrigated rice fields. SA was
of MT gene was up to 30 fold higher in transgenic observed with ameliorative effect on rice seedling under
lines. Arabidopsis plants transformed with MT gene drought condition especially improvement in water use
showed longer roots, better efficiency of survival and efficiency (WUE) and antioxidative system in the
germination, larger siliques and higher biomass present study. SA was found to enhance the activities
compared to WT. The physiological variables (A, WUE, of antioxidative enzymes such as superoxide dismutase
G, E, qP and ETR) of WT plants were reduced with (SOD), peroxidise (POD), catalase (CA) and
drought stress which were recovered in transgenic glutathione reducatse (GR) . Present study reveals that
Arabidopsis lines. The enzymatic and non-enzymatic SA reduced oxidative damage by significant
antioxidant (APX, GPX, POD, GR, GRX, GST, CAT, enhancement in the activity of SOD (EC,
MDHAR, ASc and GSH) levels were also enhanced POD (EC, GR (EC while the activity
in transgenic lines to provide tolerance. Overall, the of catalase (EC was reduced under the
results suggest that MT gene is actively involved in the drought stress condition in rice plants. We concluded
mitigation of drought stress and could be the choice that SA regulates the activity of antioxidative enzymes
for genetic engineering strategy to overcome drought through accumulation of reactive oxygen species
stress. (ROS) and may be signal transduction mechanism in
abiotic stress tolerance in plants.
Key words: Arabidopsis, Chickpea, Drought,
Metallothionein, Physiological performance, ROS. Key words: Drought acclimation, Salicylic acid, Antioxidative
enzymes, Oryza sativa L., Sustainable agriculture.

24 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SI/P-4 observation interval as compared to 1 st and 2 nd

observation intervals. It is also interesting to know that
Evaluation of Sodicity Tolerance, high growth occurred in all Aloe species during the
Growth and Yield Performance of Aloe period of June and July. After that plant growth
adversely affected during heavy rains as well as
Species at Different Soil Sodicity dormant winter season from December to January. The
Levels leaf yield of Aloe vera Accession has been better than
Aloe vera up to pH level-5 and for rest of the species
T.S. Rahi*, Anand Prakash, Faizia Iram and Lal up to pH level 10. Leaf yield was considerable high in
Bahadur Aloe vera in the control plots to pH level 10 amongst
CSIR-National Botanical Research Institute, Rana Pratap the rest of species. Gel yield also followed the same
Marg, Lucknow-226001, INDIA Email: tsrahi8@gmail.com trend up to pH level-6 as in case of leaf yield.
Aloe species are succulent in nature with multiple Key words: Aloe species, Soil sodicity, Leaf yield, Gel yield.
importances. Five Aloe species viz. Aloe vera, Aloe
vera Accssn, Aloe maculata, Aloe ferox and Aloe
spicata were examined on naturally existing 10
different soil sodicity (pH) levels as Control (7.75-8.20),
Interactive Effects of eCO 2 and
Level-2 (8.20-8.40), Level-3 (8.45-8.55), Level-4 (8.56- Drought on Pyrethrin Content in
8.68), Level-5 (8.70-8.90), Level-6 (8.91-9.0), Level- Tanacetum cinerariifolium Flowers
7 (9.01-9.15), Level-8 (9.16-9.30), Level-9 (9.31-9.55)
and Level-10 (9.56-10.20). Two months old suckers 3 Farah Deeba 1 , Vivek Pandey 2 and Laiq-Ur
to 4 leaved with 10 to 15 cm height were transplanted Rahman1
in November 2016 at 75 cm plant to plant distance in 3 1
Biotechnology Department, CSIR-Central Institute of
x 3 m size bed with three replications at Distant Medicinal and Aromatic Plants, Lucknow-226015, INDIA;
Research Centre, Banthra. Hoeing, weeding and 2
Plant Ecology and Climate Change Science Division, CSIR-
uniform irrigations were done as and when required. National Botanical Research Institute, Lucknow-226001,
Plant growth observations were recorded at two INDIA, Email: farahnbri@gmail.com
months interval. After one year of plantation, mature
Pyrethrin, a natural insecticide, extracted from
leaves were harvested for the assessment of leaf yield.
the glandular trichomes on the achenes of T.
Growth data was analysed with factorial randomized
cinerariifolium flowers, is an eco-friendly approach
bloc design (RBD). It was observed when species were
to control the various insects specifically mosquitoes.
pooled together plant growth (plant height and leaf
Indiscriminate use of synthetic pyrethrins led to the
length) reduced significantly from pH level-3 onwards
development of resistance in mosquitoes and is also
as compared to control plots, but no significant
hazardous for environment and humans. Having the
difference was noticed from pH level 6 to 10 for leaf
qualities of being environmental friendly and short life
length. Number of leaves per plant decreased
time, natural pyrethrin is a good alternative to synthetic
significantly with increasing pH levels, where as widths
ones. In India pyrethrin yield is very poor which ranges
of leaves did not decline significantly up to pH level-7.
from 0.6 to 0.79% of per flower dry weight, while in
There was no significant difference for thickness of
Kenya and Australia, it is common to find clones with
leaves when data was pooled across the pH levels and
pyrethrin content of 3.0 % and more. Under present
observation intervals. Aloe vera Accession was found
changing climate scenario, levels of CO 2 in the
significantly superior for many growth traits as
atmosphere will increase ultimately leading to increase
compared to Aloe vera and other Aloe species. Width
in average global temperatures along with recurrent
of Aloe maculata and Aloe spicata was significantly
droughts. In order to analyse the interactive effects of
higher and lower respectively with other species. As
drought along with eCO2, T. cinerariifolium plants
far as observation intervals is concerned, growth traits
were grown under eCO2 conditions for observing the
of all Aloe species increased significantly from 4th
effects of eCO2 on pyrethrin content simultaneously

Abstracts 27-30 November, 2018, NBRI, Lucknow 25

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

with two regimes of drought stress i.e. mild drought diurea (EDU) as a foliar spray (300 ppm). Average
(MD; 40-50% RWC) and severe drought (SD). It was ambient ozone concentration during the experiment was
found that the flower size of the plants grown in eCO2 61.6 ppb and average ambient and elevated CO 2
condition was larger as compared to their respective concentration (eCO2) during the experiment was 412.56
controls grown under ambient conditions. Biomass of ppm and 562.53 ppm, respectively. EDU treatment
T. cinerariifolium was found to be reduced during the resulted in less lipid peroxidation along with increased
MD and SD stress in ambient as well as in eCO2 chlorophyll content, biomass and yield. EDU alleviated
conditions. However, biomass reduction was more the negative impacts of ozone by enhancing activities
prominent in ambient ring as compared to eCO2 ring. of antioxidants and antioxidative enzymes. The present
Pyrethrin contents were found to be progressively study indicates that prevailing ozone concentrations in
increased during MD and SD respectively, under eCO2 and around Lucknow city have unfavorably affected
conditions. On the contrary its content was found to various physiological and biochemical characteristics
be significantly decreased in MD and again increased of two sorghum cultivars leading to significant growth
in SD as compared to its respective control in ambient reductions. EDU application protected plants against
conditions. The amount of pyrethrin was found to be harmful effects of ambient ozone whereas elevated
higher in ambient condition as compared to eCO2 . CO2 could ameliorate adverse effects of ozone. Two
Present study reveals that eCO2 condition helps plants cultivars selected for experiments responded
to adapt under drought condition(s) simultaneously differentially at either developmental stage of life cycle.
increasing the secondary metabolite production thus PHS-111 performed better in terms of growth and
increasing its economic value as medicinal plant. enzymatic activity over VHS-999. It indicates PHS-
Key words: Tanacetum cinerariifolium, Drought stress, 111was more responsive to EDU treatment and
eCO2, Pyrethrin, Secondary metabolite. (eCO2 ).
Key words: Tropospheric ozone, EDU, Forage crops, Fibre
SI/P-6 content.

Effects of Elevated Carbon Dioxide SI/P-7

and Tropospheric Ozone on Sorghum
Phosphate Solubilization by
Mitul Kotecha 1,2* 2
, Vishal Prasad and Vivek Pandey 1
Trichoderma koningiopsis (NBRI-
Plant Ecology and Climate Change Science Division, CSIR- PR5) under Abiotic Stress Conditions
National Botanical Research Institute,Rana Pratap Marg
Lucknow-226001, INDIA; 2Institute of Environment and Touseef Fatima, Isha Verma*, Udit Yadav, Sanjeev
Sustainable Development, Banaras Hindu University, Kumar and Poonam C. Singh
Varanasi-221005, INDIA, Email: mitulkotecha@ymail.com
Division of Microbial Technology, CSIR-National Botanical
Tropospheric O3 is phytotoxic to the plants and Research Institute-226001, Lucknow, INDIA
chronic ozone exposure negatively impacts Email: poonamnbri@rediffmail.com
photosynthesis, stomatal conductance, chlorophyll,
Phosphorus (P) is one of the major bioelements
nitrogen and protein content thereby reducing yields
limiting agricultural production. Phosphate solubilizing
and biomass. Phytotoxic effects of tropospheric O3 on
fungi play a noteworthy role in increasing the
plant growth and physiological processes are often
bioavailability of soil phosphates for plants. Phosphate
ameliorated in CO2 enriched environment. A limited
(P) solubilizing fungi contribute considerably in microbial
number of studies of interactive effects of elevated
phosphate mobilization. However, effects of different
CO2 and O3 on herbaceous vegetation are conducted
abiotic stresses on P solubilization mechanisms in
and have produced variable results. The present study
Trichoderma are largely unexplored. In the present
evaluated the impact of elevated CO2 and ambient O3
study we selected a P solubilizing Trichoderma to study
on morphological, physiological, biochemical traits and
the mechanism of P solubilization under alkaline and
fibre content in two varieties of sorghum using ethylene

26 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

drought conditions. Among 33 Trichoderma isolates different locations of districts Rawalpindi, Jhelum,
(NBRI-PR1–NBRI-PR33), NBRI-PR5 was selected Attock, Chakwal and Islamabad revealed maximum
after screening for stress tolerance, antagonistic activity mean disease incidence (MDI) on potato was recorded
against phyto-pathogens and P solubilization. The in Attock (37.4%) followed by Islamabad (35.8%),
selected strain, Trichoderma koningiopsis (NBRI-PR5) Jhelum (31.76%) and Rawalpindi (30.5%) while
was characterized and identified using ITS sequencing minimum in Chakwal (20.2%). Maximum MDI on
(Accession no. JN375992). Results show that NBRI- tomato was observed in Islamabad (38.7%) followed
PR5 uses different mechanisms of P solubilization under by Attock (36.3%), Rawalpindi (34.9%) and Chakwal
in-vitro alkaline and drought conditions. NBRI-PR5 (29.6%) while minimum in Jhelum (27.5%). MDI on
produced organic acids for solubilizing insoluble tri- chillies was maximum in Attock (30.9%), followed by
calcium phosphate (TCP) at high pH stress. In drought Islamabad and Jhelum (29.5%), while minimum in
conditions NBRI-PR5 accumulated poly phosphate in Chakwal and Rawalpindi (27.3%). Since the climate
its mycelia and produced alkaline phosphatase enzyme of the region has considerable temperature variations.
for P solubilization. The study concludes that T. Daytime temperature reaches above 40oC during the
koningiopsis employs different mechanisms of P summer while ranges between 0-10oC in winter. The
solubilization in different stress conditions and therefore, average annual rainfall is abundant at 1,249 millimetres
it can be used in management of stressed soils. (49.2 in). The fungus can remain active at a range of
Key words: Trichoderma, Abiotic stress, Phosphate temperatures and remains dormant as sclerotia. The
solubilization, Bioavailability. optimum temperature ranges 24-31oC for potato tuber
development, vegetative growth in chilli and the
SI/P-8 emergence of tomato seedlings also provide optimum
temperatures for R. solani disease development; 24-
Climate Change and the Current 32oC. In addition to temperature, soil moisture greatly
Scenario of Rhizoctonia solani Incited influences the amount of R. solani inoculum in the soil
that ultimately favours the disease development.
Disease of Solanaceous Vegetables Considering the changed climatic conditions, there is a
dire need to devise mitigation strategies for sustained
Amjad Shahzad Gondal*, Abdul Rauf and Farah vegetable production.
Key words: Rhizoctonia solani, Solanaceous vegetables,
Department of Plant Pathology, Arid Agriculture University, Maximum mean disease incidence (MDI), Climate change.
Rawalpindi, PAKISTAN Email: amjad@msu.edu,
amjadshahzad@live.com SI/P-9
Solanaceous vegetables; Potato (Solanum
tuberosum L.), tomato (Lycopersicon esculentum L.) Adaptation of Guar [Cyamopsis
and chilli (Capsicum annuum L.) are the dominant tetragonoloba (L.) Taub.] Plants in
vegetable crops worldwide that are generally cultivated
Response to Abiotic Stress of Light,
in the warm or tropical climate. They are grown
throughout the year in all parts of Pakistan. The quality Temperature and Drought
and quantity of the produce is directly affected by the
climate change as host plants and their pathogens are Shiv Narayan*, Komal Pandey and Pramod Arvind
prone to erratic climatic factors like temperature, relative Shirke
humidity, rainfall and CO2 prevailed during different Plant Physiology Laboratory, CSIR-National Botanical
crop seasons. Rhizoctonia solani is an important soil- Research Institute, Lucknow-226001, INDIA
borne fungal pathogen that is well adapted to various Email:shivnarayan2388@gmail.com, pashirke@nbri.res.in
environmental conditions. It causes black scurf, Guar [Cyamopsis tetragonoloba (L.) Taub.] is
damping-off, stem canker and root rot in these crops. an annual Kharif legume capable of growing under poor
Present study documents the scenario R. solani incited fertility and grown mainly in arid and semi-arid region
diseases in the Pothohar region of Pakistan. Survey of

Abstracts 27-30 November, 2018, NBRI, Lucknow 27

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

of India. It is grown as a vegetable for human varieties grown under Free Air Concentration
consumption, forage, green manure and as a grain crop. Enrichment facility (FACE).The study would yield rice
The endosperm of guar seed contains galactomannan germplasms that are tolerant to anticipated climate
used as a food and non-food item. We studied the change as well as possible mechanistic understanding
performance of two guar varieties namely RGC-1002 of response of rice plants to combined stresses. The
and Sarada under different abiotic conditions. study would yield robust data about how rice
Photosynthesis in response to light, temperature and physiological, genetic and yield parameters will respond
water stress was measured in the month of May and to anticipated changes in the atmosphere which may
June. In photosynthesis Vs light response curve, we help improve modelling and breeding efforts in the
observe the maximum photosynthetic saturation at 1200 future.
µmol m-2s-1 of light in RGC-1002, whereas 800 µmol Key words: Ozone, Elevated CO2, Rice, Indo Gangetic Plains,
m-2s-1 of light in Sarada. While in temperature response, FACE.
the maximum photosynthesis ranged from 35-380C in
RGC-1002 and 28-30oC in Sarada. In water stress SI/P-11
condition, RGC-1002 (RWC= 68%) shows significantly
higher photosynthesis then Sarada (RWC= 58%). Our Interaction between Climate Change
studies suggest that RGC-1002 is better adopted for Drivers and Nutrient Fertility:
abiotic conditions of high light, high temperature and
water stress then Sarada at physiological level. Temperature, CO2, and Phosphorus
Key words: Guar, Photosynthetic Photon Flux Density, Water Vangimalla R. Reddy1* and Shardendu K. Singh1,2
stress, Relative Water Content.
USDA ARS, Crop Systems and Global Change Laboratory,
SI/P-10 Bldg 001, Rm 342, BARC-W Beltsville, MD, USA; 2School of
Environmental and Forest Science, University of Washington,
Impact of Elevated CO2 and/or O3 on Seattle, WA, USA Email: vangimalla.reddy@ars.usda.gov,
Rice Varieties under Open Field
Climate change is already affecting the global
Conditions natural resource base that societies depend on to
provide food, fiber, fuel, and recreational services. An
Rushna Jamal*, Mitul Kotecha, Rekha Kannaujia,
increase in the global air temperature (T) and carbon
Vivek Pandey
dioxide (CO2) concentration is associated with the
Plant Ecology and Climate Change Science Division, alterations in atmospheric chemistry such as emissions
CSIR-National Botanical Research Institute, Lucknow of greenhouse gases (e.g., CO2, methane, nitrous oxide,
226001, INDIA, Email: and chlorofluorocarbons). Temperature, CO 2, and
Human activities are contributing to increase in nutrient availability are among the major determinants
the concentrations of atmospheric carbon dioxide (CO2) of crop’s adaptation that drive their productivity. In
and other greenhouse gases, including tropospheric natural settings, the productivity of an agro-ecosystem
ozone (O3). Indo-Gangetic plains (IGP) region is one depends on the intricate balance between multiple
of the most heavily populated and polluted region of environmental drivers. Lately, our understanding of crop
India. Rice is the most important cereal crop grown in response to individual environmental factors has
this region. There are reports of stagnation and even advanced significantly, but information on multiple
decline in rice production which has been attributed to interacting factors is needed to understand full impacts
many biotic and abiotic factors. We argue that elevated of climate change on crop productivity. Even though a
CO2 and/or O3 might be contributing towards these large proportion of crop productivity globally occurs in
yield losses. In the present experiment, we are assessing nutrient-limited conditions, crop response to climate
impact of elevated CO2 (550 ppm) and/or O3 (+20 ppb) change drivers has often been investigated under well-
on growth, physiological, biochemical, molecular, yield fertilized condition. Thus, the interactive impacts of
and nutritional quality parameters of twenty four rice temperature, CO2, and phosphorus (P) fertility on crop

28 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

productivity are still unclear. To fill in this knowledge- cycle helpful in maintaining the balance of atmospheric
gap, soybean was exposed to the combinations of two CO 2 concentrations and their response to
levels of each T (optimum and elevated, eT), CO2 environmental change. Increasing primary productivity
(ambient and elevated), and P fertilization (sufficient in tropical forests over recent decades has been
and deficient, dP) throughout the season. Results attributed to CO2 fertilization and greater biomass in
showed that dP was the most detoroius to soybean tropical forests could function as a substantial carbon
vegetative growths while eT was the most detrimental sink in the future. However, the carbon sequestration
to reproductive growth. The elevated levels of CO2 capacity of tropical forest soils is uncertain and
and temperature (separately or jointly) compensated, feedbacks between increased plant productivity and
at least partially, the detrimental impacts of P deficiency soil carbon dynamics remain unexplored. Soil CO2
on vegetative growth, such as biomass production. efflux is directly related to both microbial and root
However, compensatory effects of eT under P activities and are largely controlled by environmental
deficiency completely disappeared for the reproductive factors such as precipitation, soil moisture and
development, such as pod development. This indicated temperature. Soil CO2 efflux is a major component for
that plant response to combined stresses (e.g., eT+dP) assessing the carbon sequestration capacity of the forest
is exclusive, which might not be accurately inferred ecosystems as part of the global carbon cycle. The
from results obtained when stresses (e.g., eT or dP) present study was conducted in Katerniaghat Wild Life
imposed individually. Furthermore, the impacts of a Sanctuary (KWLS) in three forest communities (Teak
given stress situation might be even more complex due plantation, Sal mixed and dry mixed forests). Diurnal
to the contrasting nature of responses between plant soil CO2 flux (µmol CO2 m”2s”1) was measured in post
attributes (e.g., vegetative versus reproductive monsoon season using automated LI-COR 8100 soil
growth).The potential implication of these results might CO2 flux system (LI-COR, USA) with attached survey
be associated with the P-fertilizer management due to chamber (8100-103; 20 cm diameter). Variations in soil
its link with the agroecosystem and environmental water potential was measured using in Psypro (Wescor
pollution. Inc., USA). Soil was sampled seasonally from two
Key words: Abiotic stress, Climate change, Interaction, depths (0-15 and 15-30 cm) at three times intervals
Photosynthesis. (morning, afternoon and evening) in each forest
community for diurnal measurement of soil water
SI/P-12 potential. We tried to understand the interaction of soil
physical and chemical properties with soil CO2 efflux
Interaction of Soil CO2 Efflux with Soil in each forest community. We observed average efflux
Parameters in Three Different Forest rates of 3.04±0.39, 3.24±0.47, 3.41±0.72 (µmol CO2
m-2s-1) in Teak plantation, Sal mixed and dry mixed
Communities in Tropical Deciduous forests, respectively. Maximum total organic carbon
Forest of Northern India (TOC) was observed in Sal mixed forest (20.28 g kg-
) followed by dry mixed forest (19.90 g Kg-1) in post-
Shruti Mishra 1 *, Soumit K. Behera 1 , L.B. monsoon season. Soil physical properties (majorly bulk
Chaudhary1, M.K. Jain2 and Vipin Kumar2 density, particle density, porosity and water holding
1 capacity) are strongly correlated with soil CO2 efflux
Plant Ecology and Climate Change Science Division, CSIR-
National Botanical Research Institute, Lucknow-226001, in comparison to soil chemical properties (majorly total
India; 2Indian Institute of Technology, Indian School of organic carbon, pH, EC, total nitrogen and total
Mines, Danbhad-826004, India Email: mishrashruti. phosphorus). The soil physical properties revealed
1989@gmail.com strong positive control over soil carbon sequestration
in tropical deciduous forests of India.
Tropical forests containing 30% of global soil
carbon (C) are a critical component of the global carbon Key words: Soil CO2 efflux, Soil water potential, Tropical
deciduous forest.

Abstracts 27-30 November, 2018, NBRI, Lucknow 29

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SI/P-13 Key words: Nutrients, TOC, Tropical deciduous forest, Litter

Assessment of Essential Nutrients in
Soil and Litter in Tropical Deciduous SI/P-14
Forest of Northern India Impact of Elevated Ozone on Leaf
Nutrient Properties of Leucaena
Shruti Mishra 1 *, Soumit K. Behera 1 , L.B.
Chaudhary1, M.K. Jain2 and Vipin Kumar2 leucocephala: A Fiber Yielding Tree
Plant Ecology and Climate Change Science Division, CSIR- Pratiksha Singh 1,2 *, Ashish Tewari 2 and Vivek
National Botanical Research Institute, Lucknow-226001,
Pandey 1
India; 2Indian Institute of Technology, Indian School of
Mines, Danbhad-826004, India, Email: mishrashruti. Plant Ecology and Climate Change Science Division, CSIR-
1989@gmail.com National Botanical Research Institute, Lucknow-226001,
Uttar Pradesh, India; 2Department of Forestry&
In forested ecosystems, litter fall represents the Environmental Science, DSB campus, Kumaun University,
main pathway of nutrient transfer from the plants to Nainital-263001, Uttarakhand, India, Email: prachi68singh@
the soil through process of primary productivity. Forest gmail.com
litter plays an important role in determining nutrient
Tropospheric Ozone (O 3 ) concentration is
cycling, and maintaining ecosystem function. Essential
continuously rising due to industrialization and various
nutrients such as Co, Cu, Fe, Mg, Ni and Zn play a
anthropogenic activities. High O3 levels negatively
significant role in plant development. The litter nutrient
affect different plant species, changing their growth
content changes with plant community influence the
pattern as well as leaf nutrient properties. This study is
structure and activity of microbial community inhabiting
based on the long term impact of elevated ozone (EO3)
in the soil. In the present study, we quantified the
on leaf nutrient properties of Leucaena leucocephala
essential and non-essential nutrients in soil along with
at different time intervals (6, 12, 18 and 24 months).
litter in three forest communities [Teak plantation
Leucaena seedlings were grown under ambient ozone
(TPF), Sal mixed (SMF) and dry mixed (DMF) forests]
(AO3) and EO3 (+20ppb) rings at Free Air Ozone
at Katerniaghat Wildlife Sanctuary, India. Soil samples
Enrichment (FAOE) facility, CSIR-NBRI, Lucknow.
were collected from two soil depths (0-15 and 15-30
We estimated different leaf nutrient properties viz.,
cm) from each forest site randomly. Nutrient contents
[Carbon(C), Nitrogen(N), C/N ratio, Phosphorus(P),
like Chromium (Cr), Manganese (Mn), Iron (Fe), Cobalt
Sodium(Na), Potassium(K) and Calcium(Ca)] and plant
(Co), Nickel (Ni), Copper (Cu), Zinc (Zn), Arsenic
fiber (Crude fiber(CF), Acid detergent fiber(ADF),
(As), Selenium (Se), Molybdenum (Mo), Cadmium
Neutral Detergent fiber(NDF), Acid detergent
(Cd), Lead (Pb) were quantified in soil and litter.
lignin(ADL) under ambient and elevated O3. Under
Essential nutrients like Fe, Cr, Mn, Ni, Zn, Cu were
EO3 leaf C, N and P content insignificantly increased
highest in DMF followed by SMF and TPF in soil.
whereas Na and C/N ratio were insignificantly
Maximum Zn content was observed in TPF, while SMF
decreased in comparison with AO3 at different time
observed maximum Cu in surface soil (0-15 cm).
interval. L. leucocephala leaf Potassium was
Maximum nutrients in litter was observed in DMF
decreased significantly at18 months interval under EO3
among all forest communities. Maximum
while calcium content was significantly increased after
concentrations of Ni and Zn were observed in SMF
24 month interval. Leucaena fiber content, CF, NDF
and TPF litter. Litter carbon was highest in DMF among
and ADF showed significant reduction after 18 and 24
all forest communities. Different species composition
months interval under EO3 while ADL showed no
in three forest communities resulted in different nutrient
significant change. As L. leucocephala leaf nutrient
concentrations in soil and litter pool, which needs further
content showed no significant changes under EO3, it
long term monitoring for understanding the interaction
showed its tolerance to high O3. Low NDF and ADF
of tree association with nutrient dynamics.
values found under EO3 are desirable in forage from

30 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

the standpoint of its nutritive quality for ruminant species diversity by highest value of Shannon index
herbivores. Results showed that L. leucocephala is (2.1540). While EP was the community with very poor
tolerant to high ambient O3. species diversity indicated by lowest Shannon index
Keywords: FAOE, TroposphericO3, Leucaena leucocephala, (0.5611). The SAMF exhibited the highest equitability
Leaf nutrients, Fiber Content. index (0.7505), where the species were more evenly
distributed. The nMDS and Pearson’s correlation
SI/P-15 studies reveal that the assembling of different forest
communities is affected by the different environmental
Effect of Environmental Variables in variables such as, Total organic nitrogen (TON),
the Assembling of Tree Community in Electric conductivity (EC), Soil bulk density (BD), Soil
porosity (SP), Available phosphorous (P), Potassium
Tropical Moist Deciduous Forest of ion (K) and Water holding capacity (WHC) (edaphic)
Himalayan Terai and Air temperature (AT), Absolute air humidity (AAH)
and CO2 (climatic). The study also reported 23 site-
Omesh Bajpai1*, Venkatesh Dutta1, Rachna Singh2, specific species, which require proper conservation
L.B. Chaudhary3 and Jitendra Pandey2 measures due to their low ecological amplitude and
1 narrow range of distribution.
Department of Environmental Science, Babasaheb Bhimrao
Ambedkar University, Lucknow-226025, Uttar Pradesh, Key words: Trees, Phytosociology, Environmental variables,
INDIA; 2Centre of Advanced Study in Botany, Banaras Cluster analysis, nMDS, Dudhwa National Park, Tropical
Hindu University, Varanasi-221005, Uttar Pradesh, INDIA; moist deciduous forest.
Plant Diversity, Systematics and Herbarium Division, CSIR-
National Botanical Research Institute, Rana Pratap Marg, SI/P-16
Lucknow-226001, Uttar Pradesh, INDIA, Email:
omeshbajpai@gmail.com Emission of GHGS and Community
Tropical forests play a vital role in the global Level Physiological Profiles of Microbes
carbon and energy cycles. The region along the
is Altered by Soil Amendments
Himalayan foothills in northern India forms one of the
distinct eco-climatic regions of the world. The objectives Nishtha Mishra*, Pratibha Verma, Priyanka
of the study are to find out the different tree Chauhan and Aradhana Mishra
communities and summarize major edaphic and climatic
variables affecting the tree community assembling in Division of Plant-Microbe Interactions, CSIR-National
the tropical moist deciduous forest. The forests of Botanical Research Institute, Rana Pratap Marg, Lucknow-
226001, India, Email: mishramyco@yahoo.com
Dudhwa National Park (DNP), a good representative
of tropical moist deciduous forest of Terai region has This study was conducted to elucidate effects of
been selected to explore of edaphic and climatic long-term fertilization practices on plant growth, GHGs
variables. The cluster and non-metric multidimensional emission (methane and carbon dioxide), and microbial
scaling (nMDS) analyses grouped the forests of DNP diversity in rice field soil. The rice field selected for
into five major forest types: Sal miscellaneous forest experiments represented balanced and imbalanced
(SMF), Sal-Asna miscellaneous forest (SAMF), Low fertilization practices. Among five treatments [Control
land miscellaneous forest (LMF), Teak plantation (TP) (SC), 100% urea (SU), NPK-Zn (NPK), 50% NPK-
and Eucalyptus plantation (EP). Each forest type has Green manure-biofertilizer (NGMBF) and biofertilizer
its specific species composition and dominance. The (BF)], a significant increase in root growth parameters
monoculture nature of EP was clearly indicated by was observed in NGMBF treatments which was
highest dominance index (0.7566) followed by TP with closely followed by BF treatments. Similarly, further
dominance index 0.4963. Whereas the lowest analysis of yielding attributes also exhibits the same
dominance index (0.1659) with highest Simpson index pattern of growth where an increase in different yielding
(0.8341) was computed for LMF, indicating its highest parameters was evident in NGMBF treatment. Soil
heterogeneity. The LMF also showed the highest incubation studies showed highest CH 4 and CO 2

Abstracts 27-30 November, 2018, NBRI, Lucknow 31

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

emission on day3 with NPK treated soil in submerged recorded in the BF treatment which was followed by
condition whereas it was least in BF treated soil. NGMBF, SU and NPK. These findings suggest that
Dehydrogenase activity in soil was also analyzed and sustainable management practices like amendment
it was recorded highest in BF treated soil. The structure with BF in the soil are needed to mitigate CH4 and
of the microbial community in different soil amendments CO2emission from rice cultivation.
was analyzed through the sole-carbon-source utilization Key words: Climate change, Fertilizer, Methane emission,
profiles using ECO Biolog plates. As compared to SC, Soil microbial diversity.
maximum changes in the community structure was

32 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Session II
Biodiversity: Prospection and Conservation

Abstracts 27-30 November, 2018, NBRI, Lucknow 33

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

34 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SII/KN-1 Equator Initiative prize from UNDP at Johannesburg

in 2002.
Biodiversity and Traditional Knowledge Key words: Biodiversity, Traditional Knowledge,
in India in 21st Century Pharmaceuticals, Genes, Chemicals, Sustainable
P. Pushpangadan*, V. George and T.P. Ijinu
Amity Institute for Herbal and Biotech Products
Development, Peroorkada P.O. Trivandrum-695005, Kerala,
INDIA E-mail: palpuprakulam@yahoo.co.in
Bioprospection of Traditionally used
21st century is now acclaimed as the century of
Medicinal Plants
biology – The advancements made in Biological
N.K. Dubey
sciences if applied appropriately can transform the
biodiversity rich nations like India to economic powers. Department of Botany, Banaras Hindu University, Varanasi-
India is blessed with a uniquely rich and varied 221005, INDIA, Email: nkdubeybhu@gmail.com
biodiversity rich traditional knowledge system and India is a mega-biodiversity rich country and has
above all a literate/skilled and intelligent human capital varied climatic zones comprising approximately 17000-
is well placed to make such a transformations to 18000 species of flowering plants of which 6000-7000
happen. Knowledge-based, value-added product are estimated to have medicinal usage in folk practices.
development and its commercialization has become one In India, around 25,000 effective plant-based
of the fastest economic activities in the world. The formulations are used in traditional and folk medicine
liberalization of the global trade policies and other and the country enjoys an important position in the
economic reforms evolving currently with the global pharmaceuticals sector. From ancient times,
emergence of the United Nations Convention on people are known to use the traditional medicinal plant
Biological Diversity (CBD) and the World Trade Materia medica and their bioactive compounds for
Organization (WTO) requires a deeper study and health care purposes. Basically, the medical
understanding, especially in the light of the latest path formulations are developed from different plant parts
breaking achievements in science and technologies. The or their synthetic analogs together with their folklore
history of human civilization and development of systems. According to World Health Organization
economic systems are all inherently and inveterately report, more than 80 per cent of world’s population
interwoven with our biological resources. Economic depend on plant based medicines for their health care
activity of humankind continues to derive its sustenance needs. The traditionally used medicinal plants have a
directly or indirectly from the biological resources. The large range of therapeutic properties, inhibiting growth
unknown potentials of genetic diversity found in the of pathogens or kill them without causing toxicity to
biological organisms, particularly the plants represent the host cells Due to immense use of allopathic and
a never-ending biological frontier of inestimable value. synthetic antimicrobial drugs, microbes have developed
Genetic diversity will enable breeders to tailor crops to resistance to different antibiotics Herbal extracts and
meet the increased productivity, adapt changing climatic preparation from medicinal plants had come across its
conditions, disease resistance and also to meet the other journey from the very beginning of the 20th century.
essential needs and future aspirations of humankind. Recently, scientists are focusing to develop modern
Biogenetic resources are the primary source of valuable medicines based on the purified active ingredients
genes, chemicals, drugs, pharmaceuticals, natural dyes, through modern chemical and biological technologies.
gums, resins, enzymes or proteins of great health, Traditionally used medicinal plants are still recognised
nutritional and economic importance. India became the as common practice for cure of different diseases.
first country in the world implement article 8j as benefit Their disease curing ability is attributed to presence of
sharing of TK with a tribal community namely Kani. different phytochemicals including alkaloids, flavonoids
Kani model / Pushpangadan model of benefit sharing and terpenoids. Traditional knowledge offers the source
experiment for which Pushpangadan got the award of of new drugs developments from plants. Due to recent

Abstracts 27-30 November, 2018, NBRI, Lucknow 35

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

developments in gene technologies, many Lowland desert-steppes is well represented. The

biotechnologically rich but biodiversity poor countries establishment of the reserve is facilitating the restoration
are involved in the act of biopiracy by illegally patenting of steppes on the plains which were earlier occupied
the traditional knowledge of other countries. Hence, by fields.
there is urgent need of bioprospection of traditionally Key words: Steppe zone, Russia, Desert-steppes, Firm-bunch
used medicinal plants in order to have sovereign right grasses, Dwarf semi-shrubs.
on biodiversity.
Key words: Bioprospection, Medicinal plants, Materia SII/L-3
Climate Change and Crop Productivity
SII/L-2 : What is at Stake?
Conservation of Desert-Steppe K. Raja Reddy
Diversity in European Russia Fellow of Crop Science Society of America and American
Society of Agronomy, Department of Plant and Soil Sciences,
Irina N. Safronova Mississippi State University, Mississippi State, MS 39762,
Komarov Botanical Institute, Russian Academy of Science, USA, Email: Krreddy@pss.msstate.edu
Professor Popov Street, 2. St. Petersburg, 197376, RUSSIA Today’s world faces a great challenges producing
Email: irasafronova@yandex.ru adequate food, fiber, feed, industrial products and
A large area in the south of European Russia is ecosystem services for the globe’s 7.5 billion people.
in the steppe zone. Nature reserves are very important With nearly 76 million people added every year, we
in the conservation of steppe vegetation. The must develop agricultural food production and
Bogdinsko-Baskunchaksky Reserve was established ecosystem goods and services to meet future population
in 1997 in the South of the steppe zone. It includes hill of over 8 billion by the year 2025 and more than 9.7
Bolshoye (Big) Bogdo (150 m above sea level) and billion by 2050. Added to these stresses is a threat of
the plains surrounding lake Baskunchak. Desert- global climate change resulting from increased
steppes are the most xerophilous type of steppe (with greenhouse gas concentrations in the atmosphere and
firm-bunch grasses as dominants and dwarf semi- depletion of the ozone layer assumed due to
shrubs as codominants). Feather-grass steppes (Stipeta anthropogenic activities. Agriculture production and
sareptanae, Stipeta lessingianae, Stipeta capillatae) productivity are highly sensitive to changes in climate
are widespread on loamy and loam-sand soils. On the and weather conditions. Therefore, changes in regional
sandy soils Stipeta pennatae steppes are formed. The and global climate, particularly the climatic variability,
vegetation of the Bogdinsko-Baskunchaksky Reserve have been implicated to affect local as well as global
displays not only common but also unusual features. food, fiber and forest production and ecosystem goods
Bolshoye Bogdo has varied lithological composition, and services. Crops grown in future environments will
relief, and soil profiles. In this regard its vegetation is be subjected to a climate for which they were not bred.
very heterogeneous and represented by a large number Over 30 years, a series of experiments have been
of plant communities which may be combined into conducted in sunlit plant growth chambers to study
various series “ on limestones, Tertiary clays, and impacts of climate change factors (atmospheric CO2,
sandstones. Agropyreta desertori and Artemisieta temperature, water, UV-B radiation, nutrition) in several
tauricae communities are widespread on limestones. crops including native rangeland grass species. Our
Communities of dwarf semi-shrubs are very varied on studies have shown that more carbon was fixed in high-
Tertiary clays: Artemisieta lerchianae, Artemisieta CO2-grown plants at all levels of water and nutrient
pauciflorae, Atripliceta canae, Anabasieta canae deficient conditions and across a wide range of
and Kochieta prostratae. Communities of Agropyreta temperatures, water regimes, and UV-B levels.
fragili, Artemisieta marschallianae are associated Furthermore, developmental events such as flowering
with sandstones. On a small area a variety of Caspian- and crop maturity, and leaf initiation were relatively

36 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

insensitive to high-CO2, UV-B or nutrients and were Humanity, along with all species, is dependent on
mostly temperature dependent. Since crop growth in the planet earth. Degradation of our environment will
inderminate crops such as cotton is very plastic, affect the carrying capacity of life on the planet and
additional carbon available in a high-CO2 environment this in turn will affect the number of people that the
favored more vegetative and reproductive growth planet can support. Many of the natural resources of
under optimal conditions. Crop reproductive processes, the planet when degraded have impacts far beyond
however, were very sensitive to both higher and extreme what is commonly perceived. Due to the large body of
climatic conditions predicted in future climate (higher research that has been done, it is now clear that many
and more frequent episodes of temperatures and UV- of the world’s ecosystems are under intense pressure
B radiation). Elevated CO2 did not ameliorate the from human encroachment, the harvesting of natural
damaging effects of either higher temperatures or UV- resource and land clearing. This pressure is causing
B radiation on processes related crop yield. More the destruction or degradation of habitat and species
recently, we have been exploring genotype by and permanent loss of productivity, threatening
environment interactions to understand the variability biodiversity and with it human well-being. The
and to assist the breeders select the best lines suited in resilience of many ecosystems is likely to be exceeded
a changing climate in their breeding programs. this century by an unprecedented combination of
Key words: Global climate change, Anthropogenic activities, climate change, associated disturbances like flooding,
Food security, Future environments, Breeding programs, drought, forest fire, insects, ocean acidification and other
High-CO2-grown plants global change drivers such as land use/cover change,
pollution, over-exploitation of natural resources.
International protocols require regular monitoring,
conservation, and sustainable utilization of biodiversity.
Plant diversity characterization using India has done some exercise under NBSAP
satellite remote sensing programme though it didn’t result in a seamless
countrywide biodiversity spatial database. We took this
S.P.S. Kushwaha challenge under a jointly funded programme of the
Indian Council of Forestry Research and Education, Department of Space and Department of
Dehradun-248006, Uttarakhand, INDIA, Email: Biotechnology, Government of India and used remote
spskushwaha@gmail.com sensing and GIS technology effectively. In process, we
also made use of landscape parameters such as
Biological diversity means the variability among
interspersion and juxtaposition and multi-criteria
living organisms from all sources including, inter-alia,
analysis together with field inventory of plant diversity.
terrestrial, marine and other aquatic ecosystems and
A total of 16,577 field plots were laid in different
the ecological complexes of which they are part; this
vegetation types across India. Even species utility was
includes diversity within species, between species and
taken into account. It took 10 years to complete this
of ecosystems. Amazing forest diversity- 16 Type
mammoth job with high precision. Today country has
Groups, 46 Sub-Groups and 221 Ecologically Stable
wall-to-wall natural vegetation types, disturbance
Natural Vegetation Formations. One of the 18 mega-
diverse countries (ranked between 10th and 12th) with regimes, fragmentation and plant richness database.
No such survey has been carried out by any country
four global biodiversity hotspots (Himalaya, Indo-
of the world. Needless to mention that this database
Burma, Western Ghats and Sri Lanka, and A & N
has provided much needed impetus to biodiversity
Islands). Accounts for 7.8% of recorded species: 45,968
conservation efforts in the country.
recorded species of plants & 91,364 species of animals
comprising 11.18% flora and 7.44% fauna globally. With Key words: Plant diversity, Satellite remote sensing, GIS,
only 2.5% of the world’s land area, supports 16% of Ecosystem
world’s population 18 % of the domestic cattle

Abstracts 27-30 November, 2018, NBRI, Lucknow 37

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)


Comparative Analysis of Grazing Quantifying Forest Cover Changes in

Impacts on the Plant Diversity and Response to Climate Change using
Composition of Tungnath and Shokhrak Machine Learning Model
Alpine Rangelands in Western
Joity Hossain1* and Sopan Patil2
Himalayas 1
Environmental Forestry, Bangor University, Bangor LL57
1 2 2DG, Wales, UK; 2Catchment Modelling, School of
Anirban Roy * and B.S. Adhikari
Environment, Natural Resources and Geography, Bangor
Department of Plant Sciences, K.J. Somaiya College of University, Bangor, LL57 2UW, UK, Email: joityh@yahoo.
Science and Commerce (University of Bombay), Vidyavihar, com
Mumbai, Maharashtra, INDIA; 2Department of Habitat
Ecology, Wildlife Institute of India, Chandrabani, Dehradun,
Anthropogenic activities have been attributed as
Uttarakhand, INDIA, Email: anirbanroy247@gmail.com a dominant cause for recent warming of the climate.
Forest dynamics across the world have also been
The western Himalayan region of Uttarakhand altered due to climate change. Abiotic (fire, wind,
represents India’s one of the most biodiverse alpine drought, precipitations) and biotic disturbances (insects,
temperate region as well as a religious-cultural hotspot pathogens) have elevated more than 27% due to longer
undergoing rapid cultural and ecological change. This warm and dry conditions which are affecting forest
area represents the home of origin for many plant formation. A Random Forests (RF) based machine
groups including horticulturally valuable species of learning model was trained with historical data of land
Pedicularis, Rhododendron, Oxygraphis and cover, slope, elevation, aspect and future data of
Primula. Alpine meadows here also provide pastures temperature and precipitation to find the effect of
for sheep, mule and cow herders. Stocking levels for climate change between a selected area of Amazon
livestock here have quadrupled over the last five forest on Brazilian site and an area of boreal forest on
decades and shrubs are encroaching into many Canadian site. During training phase, coefficient of
historical rangelands. Herders’ voice concerns over determination (R2) score for Amazonian site was 0.901,
both herb-shrub encroachment and shrinking grasslands whereas score for Canadian Boreal site was 0.797.
in this study, we sought to determine: (i) Are alpine RF classification model has been revealed almost same
rangelands in Tungnath overgrazed and degraded? (ii) fate for both zone but for different reason. According
What are the local impacts of grazing on plant diversity to this study, RCP 8.5 would be more distressing
and community composition? (iii) Which environmental compare to RCP 4.5 for both study sites in both time
variables covary with these differences in species scale (2041-2060 and 2061-2080). Relatively, Amazon
composition across the grazing gradient? and (iv) How site forest cover found under significant threat due to
herbaceous communities in Tungnath (grazed) differs future climate change than boreal site. But both site
from Shokhrak (grazing prohibited under Kedarnath might lose huge diversity of tree species and cropland
Management Plan)? The results of this study are for future higher temperature. If climate shift continue
discussed in the light of the management and consistent with RCP 8.5 Amazon will lose 88%
conservation of alpine meadows of the Western deciduous species, whereas boreal site will lose 42%.
Himalayas. The genesis of this study lies in this conflict, According to model, heavy rain fall, prolong drought,
wherein it was realized that a sound understanding of forest fire will facilitate evergreen species over
the ecology of the temperate forests and alpine deciduous and crop species in tropical zone. On the
meadows and the impacts of pastoralism was needed other hand, intense and longer drought could bring less
to ensure effective management of these fragile succession rate, and pest attack, which might cause
environments. vast destruction of deciduous forest diversity in boreal
Key words: Alpine, Meadows, Grazing, Shokhrak, Tungnath, site. While shallow rooted crop might have affected
Western Himalayas. more by prolong drought, heavy rainfall in short period

38 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

would be facilitated some woody and shrub species in of Broknes Peninsula. Altogether 16 species of
boreal zone of Canada. Cyanoprokaryotes, belonging to 09 genera were
Key words: Climate change, RCP, Machine learning recorded from diverse habitat of Broknes Peninsula of
Larsemann Hills, East Antarctica as new record from
SII/O-3 this area.
Key words: Diversity, Cyanoprokaryotes, Broknes Peninsula,
Diversity of Cyanoprokaryotes from Larsemann hills, Antarctica.
Broknes Peninsula of Larsemann Hills,
East Antarctica: First Report
Assessment of genetic diversity using
Pratibha Gupta
ISSR and SCoT markers in natural
Botanical Survey of India, Ministry of Environment, Forest
& Climate Change, Government of India, AJCBIBG, Botanic populations of Ormosia robusta Baker-
Garden, Howrah-711103, INDIA, Email: drpratibha2011@ a critically endangered species from
Northeast India
Antarctica is an extreme environment and
contains many unique geological, glaciological and R.K. Nilasana Singha1, Ashiho A. Mao1*, Deepu
biological features and it is a gift of nature in natural Vijayan1 and S.K. Barik2
forms but due to changes in climatic conditions, we 1
Botanical Survey of India, Eastern Regional Centre,
can see the effect of Global environmental changes in Shillong-793003, INDIA; 2National Botanical Research
Antarctica. The Larsemann Hills area are located Institute, Lucknow-226001, INDIA, Email: nilasana@
approximately halfway between Vest fold Hills and gmail.com, aamao2008@gmail.com
Amery Ice Shelf on South-eastern coast of Prydz Bay
which includes two main peninsulas, the western named Ormosia robusta is a perennial tree species
Stornes and the eastern named Broknes. Human belonging to the family Fabaceae. The species is
activities in this area are promoted by the coastal location critically endangered and has been reported from India,
and ice free landscape. The further scientific research Bangladesh, Myanmar, and Thailand. In India, this tree
and the potential for tourist visits may affect the species was reported from Arunachal Pradesh and
cyanoprokaryotes (cyanobacterial) diversity. The Assam. Recent field surveys led to the discovery of
Antarctic environment is highly sensitive and new distributional records of this critically endangered
susceptible to the impacts of human activities and has species from Itanagar and Pasighat (Arunachal
much less natural ability to recover from disturbance Pradesh), Balpakhram and Baghmara (Garo hills,
than the environment of other continents. These Meghalaya) and Lengpui (Mizoram). Because of its
changes ultimately affect the growth and diversity of small population size with only ten individuals, there is
Cyanoprokaryota. Cyanobacteria are ubiquitous, a possibility of low genetic variability among the
microscopic, inhabiting the world’s oceans, freshwater individuals. It is therefore essential to study the genetic
bodies, soils, rocks, etc. and are responsible for most variability within as well as among different populations,
of the global production of organic matter by to have an insight into the possibility of genetic drift. In
photosynthesis. Thus, they play a fundamental role in the present study, we used inter-simple sequence
the world’s ecosystems and their kaleidoscopic repeats (ISSR) and started codon targeted (SCoT)
diversity, systematic and phylogeny is indispensable. fingerprinting to study the genetic diversity of inter-
Cyanoprokaryotes play a very important role in and intra-population variability of O. robusta collected
monitoring the water quality and there are prospects from five locations. The screening was carried out using
of utilisation of cyanoprokaryotes genetic stock in 20 ISSR and 20 SCoT primers. The clear and
multiple significant ways. As Stornes peninsula comes reproducible DNA fragments generated from 17 ISSRs
under the Antarctic Specially Protected Area (ACPA) and 16 SCoT primers, ranged from 200 bp to 800 bp.
hence the samples were sampled from diverse habitat The profiles of all the ten plant samples collected from

Abstracts 27-30 November, 2018, NBRI, Lucknow 39

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

five locations viz., Itanagar (1), Pasighat (2), Baghmara systems, and shared species and finally to create
(1), Balpakhram (5) and Lengpui (1) displayed a high wetland reserves.
level of polymorphism among them, indicating wide Key words: Environment, Inter-governmental, Ramsar
genetic variation, albeit their small number. convention, Treaty, Wetlands.
Key words: Critically endangered species, Ormosia robusta,
inter- and intra- population diversity, ISSR and SCoT markers SII/O-6

SII/O-5 Development of DNA Barcodes for

Delineating Representative Mangrove
The Ramsar Convention – A Bid to
Species of West Coast India
Conserve the Wetlands of National
Importance Kundan Kumar* and Ankush Ashok Saddhe
Department of Biological Sciences, Birla Institute of
Arti Garg Technology & Science Pilani, K.K. Birla Goa Campus,
Botanical Survey of India, Central Regional Centre, 10- Goa-403726, INDIA, Email: kundan@goa.bits-pilani.ac.in
Chatham Lines, Allahabad-211002, INDIA Email: Plant DNA barcoding is complex and require
kad_arti396@yahoo.com, artibsi.garg@gmail.com
more than one marker as compared to animal
Wetlands remain submerged under water for most barcoding. Mangroves are diverse estuarine ecosystem
part of the year constructing lands transitional between prevalent at tropical and subtropical zone, but
terrestrial and aquatic eco-systems. Their habitats anthropogenic activity turned them into vulnerable
embody plant succession from open waters to land and ecosystem. For conservation strategies, there is a need
vice versa, with distinct ecosystems, ecological to build molecular reference library based on molecular
characteristics, functions and values. They provide marker alongwith morphological characteristics. In the
numerous ecological services, act as migratory corridors present study, we tested the core plant barcode (rbcL
for flora and fauna and possess predominantly + matK) and four promising complementary barcodes
hydrophytic vegetation often representing primitive and (ITS2, psbK-psbI, rpoC1 and atpFatpH) in 14
highest evolved plants. Being dynamic and influenced representative mangroves species belonging to 5
by man-made and natural activities, wetlands need families from west coast of India. Data analysis was
frequent monitoring. The Ramsar Convention was based on barcode gap analysis, intra- and inter-specific
signed in 1971 as the only global, inter-governmental genetic distance, Automated Barcode Gap Discovery
environment treaty to deal with a particular ecosystem (ABGD), Taxon DNA (BM, BCM), Poisson Tree
which provides framework for national action and Processes (PTP) and General Mixed Yule-coalescent
international cooperation for conservation and wise use (GMYC). The rbcL locus showed highest PCR
of wetlands and their resources. Main objective of this efficiency and sequencing success (100%) rate,
convention was to call international attention towards followed by ITS2 and matK. Using a single locus for
depleting wetlands of international importance, provide analysis, ITS2 exhibited the highest discriminatory
framework for international discussion and cooperation power (87.82%) but combinations of matK + ITS2
toward mutual benefits, highlight wetland functions, provided the highest discrimination success (89.74%)
values, goods and services with willingness to make a rate except for Avicennia genus. Single ITS2 barcode
commitment for reversal of wetland losses. At present locus resolved Rhizophora apiculata and R.
there are 169 signatories, with India being one mucronata based on GMYC analysis and Sonneratia
comprising twenty six Ramsar sites. The Major species were demarcated using ABGD tools with
obligations of participant countries of the convention relative gap width (X=1.5). Further we evaluated 3
were to designate wetlands for inclusion in the List of additional markers (psbK-psbI, rpoC1 and atpFatpH)
Ramsar sites, promote wise use of wetlands in their for Avicennia genera (A. alba, A. officinalis and A.
territory, promote international cooperation especially marina) and of them, atpF-atpH locus was able to
with regard to trans-boundary wetlands, shared water discriminate between three species of Avicennia

40 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

genera based on ABGD and TaxonDNA analysis. Our discoveries before getting adversely affected by global
analysis underscored the efficacy of matK + ITS2 climate change and anthropogenic influences.
markers with atpFatpH as the best combination for Key words: Phycopeltis, Phyllosphere, Rural-natural forest,
mangrove identification in the west coast India region. Semi-urban, Trentepohliales.
Key words: DNA barcoding, Mangroves, West coast, India.
Status and Distribution Pattern of
Leaf Colonising Algae-Phycopeltis Native and Endemic Species on the
spp. (Trentepohliaceae, Chlorophyta) Shimla Water Catchment Wildlife
from the Southern Western Ghats, Sanctuary of Western Himalaya
Kerala, India
Dipika Rana1, Kulraj Singh Kapoor2 and Anupam
Binoy T. Thomas*, M.V. Bhagya and V.P. Thomas Bhatt1
Phycotechnology Laboratory, Department of Botany, CSIR-Institute of Himalayan and Bioresource Technology,
Catholicate College, Pathanamthitta, Kerala, INDIA Palampur-176061, Himachal Pradesh, INDIA; 2Himalayan
Email: bttkripa@gmail.com Forest Research Institute, Panthaghati, Shimla-171009,
Himachal Pradesh, INDIA Email: dipikahfri@gmail.com
The study is the pioneer investigation of leaf
colonising algae, Phycopeltis spp. from the tropical Invasions and endemism represents the two most
ecosystems of Kerala, India. Phycopeltis is the most extreme conditions of the possible geographical
abundant and distinct representatives of epiphyllous distributions of species. The areas with high percentage
algae in the tropical ecosystems. The genus of native and endemic species indicate the high
Phycopeltis was established by Millardet and it belongs conservation value of that particular area. The bio-
to the order Trentepohliales, class Ulvophyceae and geographic provinces with high diversity of native and
the division Chlorophyta. Presence of â-carotene and endemic species show the pristine ecosystems with
haematochrome are the unique features of the order least disturbance, whereas low richness of the sensitive
Trentepohliales. While possessing a remarkable biodiversity elements indicates high disturbance and
diversity in the tropical ecosystems, the genus opening the way for the invasion of non-natives.
Phycopeltis appears under-described in this region. Therefore, the native and endemic plants in a natural
Nine species of Phycopeltis from the semi-urban and ecosystem play an important role in the conservation
rural areas of Pathanamthitta District, Kerala, India and preservation of an ecosystem. In the present times
were isolated such as P. arundinacea (Montagne) De the high anthropogenic pressure and climate change
Toni, P. irregularis (Schmidle) Wille, P. epiphyton have increased the stress on natural ecosystem and
Millardet, P. flabellate Thompson and Wujek, P. aurea leading to rapid loss of native species and proliferation
Karsten, P. vaga Thompson et Wujek, P. treubii of non-native species. Maintenance of naturalness
Karsten, P. treubioides Thompson and Wujek and P. (nativity) and uniqueness (endemism) of the ecosystem
minuta Thompson and Wujek. It was found that species has become a big challenge. The extinction of the
richness and diversity indices of Phycopeltis spp. were indigenous species has caused biodiversity reduction,
more prevalent in the natural forest and in monsoon habitat loss and alteration of natural ecological
seasons; limited in the region of semi-urban areas and processes. Introduced species pose threat to
summer season. Phycopeltis was more widespread biodiversity by direct competition with native and
on the adaxial side of the moist leaves during all endemic plant species. The present study was
seasons. The study confirms the diversity of epiphyllous undertaken in the Shimla Water Catchment Sanctuary
Phycopeltis spp. in the tropical ecosystem. The of Western Himalaya in which out of the total reported
detailed surveys of leaf colonising Phycopeltis spp. 476 species, 245 species were native to the Indian
are desirable and can be expected to find new Himalayan Region (IHR), remaining 231 species were

Abstracts 27-30 November, 2018, NBRI, Lucknow 41

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

non-natives, while 8 species were endemic and 85 literature. Total 35 persons were selected for the study
species were near endemic to the IHR. Total 312 and information of about 25 plant species was gathered.
species were quantified, of which 53.85% species were Out of 25 plant species, maximum number of plant
native, while 46.15% were non-native. 20.19% of the species recorded was herbs followed by shrubs, trees
total species were near endemic and 2.56% of the total and climbers. Leaves comprised the major plant part
was endemic to the IHR. 71.43% trees, 66.15% shrubs used. Information gathered can be used in search of
and 47.94% herbs were natives, whereas among these new herbal medicines by the researchers in the field
native species, 40.00% trees, 27.90% shrubs and of modern drug development.
29.52% herbs were near endemic. Among the native Key words: Traditional knowledge, Healthcare, Kapurthala,
species 4.65% shrubs and 3.80% herbs were endemic Punjab.
species. The altitudinal distributions of the native, non-
native and endemic species were also evaluated. SII/O-10
Key words: Indian Himalayan Region, Catchment, Sanctuary,
Endemic, Near Endemic, Native. Flower Traits and its Variability as an
Adaptive Strategy for Augmenting
Reproduction in Plants Growing in
Traditional Knowledge on the Utiliza- High Altitude Environments
tion of Plant Species for Health Care 1 1
Mustaqeem Ahmad *, Sanjay Kumar Uniyal ,
Jaskirat Kaur*, Rajinder Kaur and Avinash Kaur Daizy R. Batish2 and H.P. Singh3
Nagpal 1
High Altitude Biology Division, CSIR-Institute of Himalayan
Department of Botanical and Environmental Sciences, Guru Bioresource Technology, Palampur-176061, HP, INDIA;
Nanak Dev University, Amritsar-143005, Punjab, INDIA Department of Botany, Panjab University Chandigarh-
Email: jaskiratk888@gmail.com, swab2002@yahoo.com, 160014, INDIA; 3Department of Environmental Studies,
avnagpal@rediffmail.com Panjab University Chandigarh-160014, INDIA
Email: mustaqeem.env@gmail.com, suniyal@ihbt.res.in,
The present study was aimed at exploring the daizybatish@yahoo.com, hpsingh_01@yahoo.com
traditional knowledge of local inhabitants of Kapurthala
Flower traits are key to reproductive success of
district on the utilization of plant species for health care
a plant species. Along an altitudinal gradient, owing to
management. Since times immemorial, plants have
limited growing season and thereby pollination success,
been used for various purposes by human population.
flower traits become crucial. Therefore, flower display
About 80% of the total world population is depending
area (FDA), flower longevity and resource investment
upon plants for their health care. The traditional
should be higher in plant populations growing at
knowledge of local inhabitants has been passed from
higheraltitudes as compared their low altitude
generation to generation mostly through oral
counterparts. We hypothesized that altitudinal variation
communication and in doing so part of it might be lost.
of flower traits handles reproductive success of plants.
So there is growing interest in documentation of
To test this, a study was conducted along an altitudinal
traditional knowledge. People rely on plants for many
gradient of 2000 to 4000 m asl in the Dhauladhar
health problems like fever, cough, diarrhea, constipation,
mountain range of western Himalaya during May
cuts and wounds etc. This study was based on
2015to September 2017. Along this altitudinal gradient,
questionnaire based personal interviews, field study and
twenty-one, 20×20 m permanent plots were established,
direct observations. Successive field surveys were
one each at every 100 m rise in altitude.Twenty-five
carried out from April 2015 to May 2016 mainly in
plant species were identified and 10 to 20 individuals
search of traditional healers or practioners. Information
of each were collected from the marked plots. Only
was also gathered from house wives, farmers, teachers
healthy looking, fully mature plants were collected for
and elderly people of the study area. Information was
flower trait (display area, mass and flower count)
also cross checked for authenticity from the available
analyses. After fruiting, seed count and seed mass were

42 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

also recorded for the selected 25 species. These were on the protection of Chure through initiation of
used as indicators of reproductive fitness. The ‘Rastrapati Chure Conservation Program’ since 2009.
relationship between altitude and traits for every It is an attempt to identify the problems, challenges
population sample were determined with a Pearson’s and issues of Chure forest conservation and to propose
parametric product movement(r) test at 95% an effective conservation plan using qualitative as well
confidence. To test the effect of elevation on as quantitative methods of analysis. Chure region has
reproductive traits, linear model of ANOVA was used. many opportunities of employment and income
The results revealed that except for specific flower generation through establishment of environment
area and seed count, reproductive traits positively and friendly green enterprises. Thus it is an urgent need to
significantly correlated with altitude. Seed traits were formulate short-term to long-term strategies with policy
associated with flower traits wherein FDA and flower priorities actions and result-oriented efforts in order to
mass were of prime importance. Increased flower establish Chure as a rich bio-diversity zone, with
visibility seems to ensure pollination while larger seed hazardless and improved livelihoods of its people. To
size in populations of high altitude species gives them a bridge this research gap, this research is on Community
chance to survive in harsh environments. We suggest and Collaborative Forestry contributing role toward
flower traits variations along altitude to be helpful for Chure development and Conservation completed. The
reproductive success of plants. paper discusses how development and conservation
Key words: Himalaya, Flower traits, Altitude, Reproductive initiatives carried out in the chure areas from the
fitness, Adaptations. Community based Forest Management practice. This
paper also principally discusses the lessons learned on
SII/O-11 Community and Collaborative Forests complementary
for Chure development/conservation to fulfill the broad
Impact of Community based Forest objectives of the President Chure Terai Madhesh Plan
Management (Community/Collaborative and millennium development goals. Based on the
conclusion that action research several recommenda-
Forestry) System in the Chure region tions for better management of Community based
Conservation and Development of Nepal Forest. At present forestry database show that 2.00
million households or 37 % of the population of Nepal
Damodar Sharma is involved in community forestry management program,
19361 Community Forestry User Groups (CFUGs)
Democratic Foresters’, Association of Nepal, Kathmandu,
NEPAL, Email: damodar.dfan@gmail.com have been formed 1,813478 hectares of National forest
have been handed over as community forests and
There is a growing concern regarding the 2,250,858 households have benefited from CF. It has
conservation and development of Chure hill of Nepal, been estimated that 3.75 million hectare of forest land
although land degradation of the Chure area of Nepal of Nepal can be put under Community Forestry.
is primarily contributed by different types of landslides Whereas there are 28 Collaborative Forest
and mass wasting phenomena including flooding and Management groups, who are managing more than
inundation. Landslides cause a huge loss of human life 71,000 ha Forest. There are three major partners
and property as well as environmental degradation in responsible to protect and manage Terai forest,
Nepal requiring extra resources for relief and recovery. including Department of Forest (DOF), local
Chure region is extended up to India, Bhutan & government and local communities in CFM. CFM is a
Pakistan from Nepal, covering 33 districts of Nepal concept, which has emerged after the 2000 A. D. It
with 12.6 % of country area. It is the youngest mountain has been estimated that more than 4.5 million hectare
of the world, and suffering from mass erosion, of forest land of Nepal can be put under Community
landslides and other environmental externalities which based Forestry. Currently, this management system is
make the region vulnerable. The ecological niche in recognized by forest policy, 2015 and Forest Act (1993)
and around the region makes the region more sensitive 2nd amendment 2017. Community based Forestry
and fragile. Government has given greater emphasis Management is being tools to address the issues of

Abstracts 27-30 November, 2018, NBRI, Lucknow 43

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

users including nearby as well distant users, who have garden size and elevation range; generally the small
as well have not access and control over national forest gardens had maximum species diversity than the larger
since last 7 decades because of government policies garden while lower altitude had higher species richness
and programs. At the same time, there are numbers of than the higher altitude. The multistrata nature of most
issues and challenges emerged due to the unclear roles home gardens further reveal their ecological
and responsibilities among three partners. The newly significance in providing suitable niches to various
enacted “Local Government Operating Act 2074” has species at different altitudes. The species that were
also created confusion in the operation of Collaborative grown in the gardens, to some extent, were related to
Forest Management. Likewise, benefit sharing ratio is the socio-cultural and demographic factors of the home
also not clear so far. According to the New Constitution, garden owners and associated traditional knowledge
numbers of laws are being formulated or in the process in managing these resources. Many of the landraces,
of amendment. Capacity building, local level rare and endangered species were found domesticated
development, livelihood promotion as social in the garden. These findings reveal that home gardens
development; Income generation activities through are production system maintained purposively for
forest base enterprises with employment generation harvesting diverse products for food and nutritional
as economic development and water source and soil security and are important avenues for species
conservation as well as alternative energy as conservation at household level.
environment conservation were found fruitful in the Key words: Homestead agroforestry, Biodiversity
Chure and surrounding Terai area development. conservation, Nutritional security, Mizo community.
Key words: Socio-economic Impact, Development spectrum,
Community based forestry, Community Forestry, SII/O-13
Collaborative Forestry, Chure Conservation.
Medicinal Plant Diversity in Chandra
SII/O-12 Prabha Wildlife Sanctuary, Chandauli
Traditional Agroforestry for District, Uttar Pradesh
Biodiversity Conservation, Food and Nitisha Srivastava* and Achuta Nand Shukla
Nutritional Security of Mizo Botanical Survey of India, Central Regional Centre, 10,
Community of Northeast India Chatham Lines, Allahabad-211002, Uttar Pradesh, INDIA
Email: srivastava_nitisha@yahoo.com
Uttam Kumar Sahoo* and Soibam Lanabir Singh India is floristically rich country and is recognized
Department of Forestry, School of Earth Sciences & Natural in twelve mega biodiversity centers in the world. It is
Resource Management, Mizoram University, also blessed with rich medicinal plant diversity. Forests
Aizawl-796004, Mizoram, INDIA, Email: uksahoo_2003@ are the major sources of medicinal plants; besides these
rediffmail.com, uttams64@gmail.com they are also cultivated. Protected areas play a very
We evaluated 137 home gardens for their species significant role in conservation of medicinal plants and
composition and product outputs and use by the Mizo traditional knowledge. Chandra Prabha Wildlife
community, the study covered five districts of Mizoram. Sanctuary (CPWLS) is situated in the district
As many of 460 plant species belonging to various user Chandauli. The presented study was carried out in the
groups were encountered in the study, the majority of area of CPWLS for survey and collection of medicinal
the plant species belonged to vegetables, fruits and plants. Information on medicinal properties of plants
medicinal plants followed by firewood, ornamental encountered in the present study was generated using
species, timber and other category. Many other utility various relevant literatures. A total of more than 120
species such as spices, beverages, broom, handicraft medicinally important plant species were reported.
etc were also widely reported from the gardens. Medicinal plants of CPWLS belong to various families.
Nevertheless the composition of species varied with Out of these many plant species fell into the rare,

44 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

endangered and vulnerable status categories. The SII/O-15

present study aimed to document the traditional uses
of different plant parts of medicinal plants. This study Phylogeography and Population
can serve as baseline information on medicinal plants Genetics of Common Reed (Phragmites
and could be helpful to further strengthen the
conservation of this important resource. australis) an Obnoxious Aquatic
Key words: Medicinal plants, Chandra Prabha Wildlife Invader in India with Comparison of
Sanctuary, Rare, Resources. Native Populations in Canada
SII/O-14 Gowher A. Wani1,2*, Manzoor A. Shah1, Zafar A.
Reshi1 , Marie-Ève Beaulieu2, Alain R. Atangana2
History of Bougainvillea: A Long and Damase P. Khasa2
Journey from Brazil to India 1
Department of Botany, University of Kashmir, Srinagar-
190006, J&K, INDIA; 2Centre for Forest Research (CEF) and
S.C. Sharma Institute for Integrative and Systems Biology (IBIS),
CSIR-National Botanical Research Institute, Lucknow- Université Laval, Québec, QC G1V0A6, CANADA
226001, INDIA, Email: scsharmagardener@gmail.com Email: gowhar.wani88@gmail.com

Bougainvillea is one of the most colourful, Whether invasive species vary in morphological
drought resistant and pollution tolerant climbing shrubs. traits and at molecular level across native and non-
Their colourful bracts make the Bougainvillea as the native regions, though much speculated, is not
preferred choice for the landscaping of parks and supported by adequate quantitative data. Furthermore,
gardens in tropical and sub-temperate regions and the origin of differences in traits influencing competitive
known as ‘Glory of the Tropics’. Bougainvillea is the success between exotic and native wild populations of
member of the family Nyctaginaceae. Bougainvilleas alien species is subject of debate. Therefore,
have attained popularity due to their bracts, which deciphering the driving factors among-population
appear in various colours and shades. The plant was divergence and the relative contribution from natural
first collected by a French Botanist, Commerson in selection and neutral genetic differentiation is important
1769 from Rio de Janeiro (Brazil). Commerson named in evolutionary biology. On the basis of morphometric
the plant after Lois Antoine de Bougainville the French traits, we compared five putative native populations of
Navigator, with whom he went on a voyage round the Phragmites australis in Quebec, Canada (haplotype
world. History of Bougainvillea is ca 250 years old. QN), six putative populations exotic to Quebec
It has travelled a long distance from Brazil to India. (haplotype QE), and five populations invasive in
There are ca 350 Bougainvillea cultivars of Kashmir, India (haplotype KE), for seven quantitative
Bougainvillea all over the world. 50 % of the traits. CpDNA PCR-RFLP analyses identified only one
Bougainvillea cultivars have been evolved in India. putative native P. australis population in Quebec. Using
India has been recognized as the International ten chloroplastic DNA microsatellite loci, we also
Registration Authority for Bougainvilleas by the compared nine populations each of haplotypes KE and
International Society for Horticulture. CSIR-National QE, and one population of haplotype QN. Among- and
Botanical Research Institute (NBRI), Lucknow is within-population variation was significant for all
maintaining an unique rich germ-plasm collection of measured traits, except for leaf width.Variation between
250 species/cultivars in the Botanical Garden. NBRI haplotypes was highly significant for leaf width.
is well recognized for its R & D work on Invasive haplotypes showed the highest values for the
Bougainvilleas. Institute has evolved ca 30 novel characters that were studied. Phenotypic differentiation
Bougainvillea cultivars, which remain in high demand among populations was moderate to high. The ten loci
in the floriculture industry. Bougainvillea has flourished were variable, and moderate levels of genetic diversity
very well in India than its centre of origin. were found within populations. Moderate genetic
differentiation was observed between haplotypes and
Key words: Bougainvillea, Commerson, Brazil, Louis Antoine
de Bougainville.

Abstracts 27-30 November, 2018, NBRI, Lucknow 45

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

populations, indicating occurrence of gene flow correlation between species density (-1.0) and
between populations. Principal coordinates analysis frequency (-0.81) with total carbon density. However,
clearly distinguished three haplotypes. Phenotypic strong positive correlation (0.74) was observed
divergence exceeded genotypic divergence, indicating between basal area and carbon density reflecting the
divergent selection amongst populations. The invasive contribution of the forest with higher girth trees towards
populations in Kashmir and Quebec harboured highest biomass and carbon stock which further necessitates
proportion of common alleles of comparable size, and the conservation of such forests for carbon
belonged to similar haplotypes. Furthermore, their sequestration.
polyploidy nature suggests the European nativity of Key words: Natural resources, Carbon sequestration,
Kashmir populations. Biomass and carbon, Forest types, Conservation.
Key words: Common reed, CpDNA microsatellite loci,
Haplotypes, Phenotypic and genetic differentiation, Plant SII/O-17
Environmental Determinants of Plant
SII/O-16 Richness in the Eastern Himalaya
Species Diversity and Carbon Stock in Rajendra Mohan Panda* and Mukunda Dev Behera
Three Temperate Forest Types of Garhwal Centre for Oceans, Rivers, Atmosphere and Land Sciences
Himalayas, Uttarakhand, India (CORAL), Indian Institute of Technology, Kharagpur,
West Bengal, INDIA, Email: rmp.iit.kgp@gmail.com
Siddhartha Kaushal and Ratul Baishya* Understanding underlying causes of high plant
Department of Botany, Faculty of Science, University of diversity is imperative for mountain ecosystems. The
Delhi, Delhi-110007, INDIA Email: kaushal.siddhartha@ study on the Indian Eastern Himalaya assesses effects
gmail.com, rbaishyadu@gmail.com of physiographic, climate, edaphic and disturbance on
With immense pressure on natural resources due plant richness of a national database. 1475 species from
to unsustainable rapid development and anthropogenic 411 geospatial locations between elevation ranges 129
activities, climate change is the worst fear of the and 4412 m were considered. Eight out of 35
century. The exponential carbon emissions have environmental variables were selected after optimisation
prioritized our goals to curb this rise and thus Carbon involving multicollinearity test and principal component
sequestration is nature’s answer to this problem. The analysis. Generalised additive model (GAM) cross-
state of Uttarakhand has 45.43% forest cover and offers validation results were validated using Ordinary least
various forest types because of climatic and geographic squares and structural equation model (SEM) for
variations offered by the Himalayas. The present study independent and multivariate analysis, respectively.
aims to report the species diversity in three temperate Potential evapotranspiration is found to have a
forest types (2144-2285m) of Garhwal Himalayas along significant climatic predictor, with an explained deviance
with tree species biomass and carbon stock. The stem of 22.5% and showed positive correlation with plant
density was observed between 275-360 trees ha-1 with richness. Precipitation seasonality and temperature
total tree biomass density ranging between 480 to 859 seasonality displayed negative correlations with plant
Mg ha -1 and corresponding carbon stock density richness. Elevation could explain 21.9% deviance,
between 220-397 Mg C ha -1 . Species dispersal for whereas cation exchange capacity could describe the
majority of the species in all the three forest types was maximum deviance by any variable i.e., 24%. The best
of contagious type. Simpson’s dominance index showed model could describe 51.1% in plant richness with
strong positive correlation with both elevation (0.79) contributions from climate, physiography, soil and
and total carbon density (0.78), while species richness disturbance. SEM grossly underestimated the GAM
(SR) showing negative correlation with both indicating results and described 26% variance of the best model
reduction of diversity with altitude. The indicating superior execution of nonlinear settings to
phytosociological data indicated a net negative species-environment relationships. Decision tree

46 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

described increase in temperature seasonality (> 476.8 0.50. This indicates the high acceptance of these plants
CV) could enhance plant richness at PET > 1163 ha in the primary health care. We also discussed the
yr-1. Precipitation seasonality (d” 82 CV) could increase degree of invasiveness and habitat preference of these
plant richness with decrease in temperature seasonality species. The use of invasive alien plant species relieves
(d” 476.8 CV). Precipitation seasonality (value > 109 the pressure on the native plant species that leads to
mm) and precipitation (> 1719 mm) could increase plant the native plant diversity conservation. Finding of this
richness with rise in temperature seasonality (> 463.6 study can be used as an ethnopharmacological basis
CV). Precipitation seasonality (> 93 CV) and for selecting plants for future phytochemical
precipitation (> 2983 mm) could reduce plant richness pharmacological studies.
further, even at low level of temperature seasonality Key words: Ethnomedicine, Invasive, Tribal, Jhabua.
(463.6 CV). It infers that climate defined by water-
energy dynamics and its heterogeneity act in synergy SII/O-19
in describing plant richness. Comparison of different
modeling approaches is suggested to crosscheck Aconitum duclouxii H. Lév.
validity of model selection.
(Ranunculaceae)- A New Record for
Key words: Eastern Himalaya, Plant richness, GAM, SEM.
the Indian Flora
Danish Husain1,2*, Priyanka Agnihotri1,2and Tariq
Assessment of the Diversity of Invasive Husain1,2 *
Alien Plants and its Role in Ethno- Plant Diversity, Systematics & Herbarium Division, CSIR-
National Botanical Research Institute, Rana Pratap Marg,
medicine in Western Madhya Pradesh, Lucknow-226001, INDIA; 2Academy of Scientific and
India Innovative Research (AcSIR), Ghaziabad-201002, INDIA
Email: husainmohddanish@gmail.com
Vijay Vishnu Wagh The genus Aconitum L. (Ranunculaceae)
Plant Diversity, Systematics and Herbarium Division, CSIR characterized by hooded zygomorphic flowers with
–National Botanical Research Institute, Lucknow-226001, hidden nectaries and characteristic seed-coat
Uttar Pradesh, INDIA, Email: vijaywagh65@gmail.com ornamentation comprises of ca. 300 species worldwide
and is represented by 27 species and 5 varieties from
Tribal community of Jhabua district uses the forest
India. This highly medicinal genus is confined to
resources especially plants primarily for curing various
Himalayan region and taxa are well represented in both
ailments. Ethnobotanical practice has prevailed in this
western and eastern Himalaya. While examining the
area since the ancient time and the invasive plants are
herbarium vouchers from The Natural History Museum,
not the exception. Exhaustive field surveys were
London (BM) during the revisionary study on the tribe
conducted for the collection of the ethnobotanical data
Delphineae, we came across a specimen of Aconitum
and voucher specimen. Information regarding
sp. collected by F. Kingdon-Ward (Coll. No. 20246)
ethnobotanical uses of plants was collected from the
in 1950from Walong, Lohit Valley, Assam (now
tribals using a semi-structured questionnaire. Several
Arunachal Pradesh) at an altitude of 10,000 ft. asl.
extensive reviews which studied invasive plant species
This herbarium voucher has been identified and
are available. From this survey, a total of 102 plant
annotated as Aconitum duclouxii H. Lév. by Y.
species belonging to 38 families were reported for
Kadotain the year 2000. After more closer studies and
curing 37 types of ailments. Asteraceae was the
critical examination of the specimen, we could conclude
dominant family and in life form category herbs stand
that the annotation by Kadota is agreeable and literature
dominant. Leaf is the most frequently used plant part,
surveys confirm that the taxon has never been reported
where as decoctionis the highly preferred preparatory
from Indian Himalayan Region, thus, confirming a new
method for medicine preparation in the study area. 56%
record for Indian flora. Previously, the taxon was
of the invasive plants showed a use value of more than
known endemic to Yunnan, China and this study show

Abstracts 27-30 November, 2018, NBRI, Lucknow 47

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

the extended distribution of the taxon in Arunachal series of benefits to the local, regional, and national
Pradesh which is adjacent to Yunnan region, thus making populations and include raw materials, patrimony
the new report from India. A. duclouxiiis closely allied protection, and scenic beauty.
to A. nagarum Stapf and both of them belong to Key words: Fundamental, Nutrition, Medicines, Fuel,
Aconitum subgen. Aconitum, a group of biennials with Physicians, Protection.
paired tuberous roots, but differs from the latter in
having pedicels with spreading glandular hairs, SII/O-21
bracteoles broader, carpels with hirsute golden hairs,
petals with a comparatively shorter lip, and petal-lip Documentation of Pollinator Floral
with inconspicuous veins and entire, non wavymargins. Calendar: A Case Study from Kashmir
Key words: Aconitum, Assam, Ranunculaceae. Himalayas
Zubair A. Rather 1*, Anzar A. Khuroo 1, Shoukat
Role of the Plants Used in the Ara2, Rameez Ahmad1 and Maroof Hamid1
Centre for Biodiversity and Taxonomy, Department of
Traditional Medicine Botany, University of Kashmir, Srinagar-190006, J&K, INDIA;
Research and Training Centre for Pollinators, Pollinizers
Mukul Kumar and Pollination Management, Sher-e-Kashmir University of
Department of Botany and Plant Physiology, M.B.A.C., Agricultural Sciences and Technology, Srinagar-190025, J&K,
Agwanpur, Saharsa, (B.A.U. Sabour), Bihar, INDIA INDIA, Email: zubairrather4@gmail.com
Email: drmukul.bau@gmail.com
Insect pollinators play a vital role in the pollination
Plants have played a fundamental role for the of plants occurring in natural as well as human-
development of cultures ever since man first arrived dominated ecosystems. Thus the availability of
to that region approximately 10000 years ago. Through sufficient forage resources, pollen and nectar,
history, man has utilized vegetable resources as a source throughout the calendar year is the pre-requisite for
of nutrition, medicines, fuel, and building materials and the sustainable population of pollinators, and the
they even occupied an important place within their productivity of pollinator-dependent ecosystems. The
system and rites. The use of plants for treating diseases present study documented the insect pollinator and
keeps being necessary Tokoshi zone in Bihar, which, flower interactions in different seasons during the
due to high costs, have a difficult access to western years2012-2016 in Kashmir Himalaya. Based on
medicines and, therefore, use to really on their own systematic surveys and field observations, at each site,
traditional remedies. On the other hand, the integrated interactions were recorded at five points along 30m
forms of modern and traditional medicines are often trail by moving in parallel directions for 60 minutes.
practiced by many physicians nowadays. This has given Flowering period for a particular pollinator foraging plant
origin to a popular medicine comprising the use of more species was observed from the date of opening of first
than 100 local plants, which have been botanically flower to the date of shedding of last flower. Our results
identified. The illnesses cured with the plants are of suggested that majority of forage resources are
course, strictly related with the health situation of local available in the month of May (84%) followed by June
communities. Wound healing aids, renal and stomach (70%), July (53%), April (46%), whereas the insect
infections, headaches, and colds are the most common pollinators especially managed honey bees suffer for
ailments which natural resources are used for. forage resources in the winter months of December,
Nonetheless less frequent diseases, such as cancer, January and February during which only 3.77%, 4.71%,
diabetes, and malaria, are also treated with different 8% forage resources are available respectively. The
plant extracts. According to the World Health present study provides pollinator floral calendar of
Organization about 80% of the world population uses frequently visited forage plants and the period of their
natural remedies and traditional medicine. Such availability for the pollinators in different seasons across
medicine comes from plant resources that provide a the year. The results from the present study will help

48 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

in better management of honey bees in apiculture and resulted in maintenance of a thick Cedrusdeodara
also continuous availability of pollinators for sustainable forest from which no resources are extracted except
productivity in agriculture and horticulture in this in emergencies. For water, setting up of baudies
Himalayan region. (water reservoirs) and devtaals (sacred ponds) such
Key words: Pollinators, Pollination, Floral calendar, as Dynasur are pertinent examples. Polluting them is
Himalayas, Agriculture. a sin. For soils, terraces and bund formation are
important practices. Ghasnis- for grassland protection
SII/P-1 ensures sustainable availability of fodder during lean
season. In a nut shell, besides using biodiversity, the
Traditional Uses of Biodiversity Bhangalis are also involved in practices that guide its
amongst the Bhangalis and the conservation. Unfortunately, these practices are
breaking under the influence of market forces and
Practices for their Conservation changing lifestyle. Thus, strategies for their
documentation, preservation and maintenance become
Alpy Sharma1 *, Sanjay Kr. Uniyal2 and Daizy R. important.
Key words: Bhangalis, Biodiversity, Conservation,
High Altitude Biology Division, CSIR-Institute of Himalayan Management, Market.
Bioresource Technology, Palampur-176061, Himachal
Pradesh, INDIA; 2High Altitude Biology Division, CSIR- SII/P-2
Institute of Himalayan Bioresource Technology, Palampur,
HP 176061 INDIA; 3Department of Botany, Panjab University, Poplar and Other Fast-Growing Tree
Chandigarh-160014, INDIA, Email: sharmaalpy@gmail.com,
suniyal@ihbt.res.in, daizybatish@yahoo.com Culture Outside Forest
Recognizing the importance of biodiversity and Anita Tomar*, Anubha Srivastav and Abhijeet
its conservation, the present study was conducted to Pandey
document traditional uses of biodiversity and various
conservation practices followed by the Bhangalis- Forest Research Centre for Eco-rehabilitation, 3/1, Lajpat
residents of Chhota Bhangal. The Chhota Bhangal lies Rai Road, New Katra, Allahabad, Uttar Pradesh, INDIA
Email: anitatomar@rediffmail.com
in the lap of Dhauladhar mountain range, Himachal
Pradesh. Field surveys were carried out to the area Plantation forestry outside the forest in India is
and personal interactions were held with the Bhangalis largely based on fast-growing trees. This is particularly
to record use of wild plants for edible purposes. The true for agroforestry which is practised by individual
study also targeted documentation of plants derived growers in their farmland and wasteland. Trees are
household products and the traditional conservation systematically planted in farmland as block plantation
practices prevalent in the area. The results revealed or boundary plantation, or maintained as scattered trees
use of 50 wild plant species for edible purposes. for various tangible and intangible benefits. The Forest
Maximum of these were used as vegetable (mean=8.9) Survey of India has estimated the growing stock of
while the minimum was for brew (mean= 0.4). trees outside forest at 1573 billion cubic metre (27.3%
Bhangalis used 55 plants derived products of which of total growing stock). However, the annual wood
majority were used as indigenous tools (34). For making production of trees outside forest is about 13 times more
these products, 20 species belonging to the 12 families than the forest, suggesting the popularity of fast-
were used. Maximum of these belong to the family growing trees outside the forest. The boost to tree
Pinaceae and 50% of used species were trees. As planting outside the forest was provided by the National
evident, since their livelihood is dependent on Commission on Agriculture, 1976 and the National
bioresources, Bhangalis ensure their flow and Forest Policy, 1988. Eucalyptus, Casuarina, Populus,
availability by following traditional practices for forest, Dalbergia, Tectona, Melia etc. are among the most
soil, water, and grassland conservation. Presence of a popular plantation trees. Despite this, several policy
sacred grove dedicated to local deity Ajayapaal has interventions are adversely affecting the interests of

Abstracts 27-30 November, 2018, NBRI, Lucknow 49

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

tree growers. Difficulties in obtaining felling and transit weight, lenticels, days of harvest and fruit per plants)
permits, absence of a favourable minimum support price and information on vegetative as well reproductive
mechanism, non-availability of soft bank loans, absence (Leaf, flowers, inflorescence and fruits) parts of the
of agroforestry cooperatives, Uncertainty about Sechium were studied for its morphological characters.
marketing/ sale of end produce, Small landholding, Poor From the investigation, the collected germplasms of
technical knowhow regarding planting practices and the fruits shows great variation in its appearances
suitable tree crop combinations, inadequate availability (shapes, colour, spines distribution) and weight. The
of superior planting material, etc., pose serious plant was found to be herbaceous, monoecious,
challenge to the grower. Populus deltoides, in perennial, climber, supported by branched tendrils and
particular, witnesses large fluctuations in the price of leaves, with light to dark green fruits. Chromosome
wood which renders the planting rate of this species count are tried to establish correct chromosome number
highly variable from year to year. A low level of in all the 14 genotypes as well as ploidy level and
technical knowledge about tree cultivation too deviations, if any. Since the distribution of the species
sometimes results in losses to growers. Insufficient is restricted to a limited area across the world, it is
research grant is also proving a handicap in technical worthwhile to preserve and conserve the species.
innovations in plantation forestry. Aninitative was taken Key words: Sechium edule (Jacq.) Swartz, Phenotype,
by Forest Research Centre for Ecorehabilitation (A Genotype, Chromosome number, Genetic diversity.
Centre of Forest Research Institute, Dehradun) for
planting fast growing tree outside forest in which Poplar SII/P-4
deltoides, Eucalyptus, Tectona grandis, Gmelina
arborea agroforestry trial are proposed in Gorakhpur, Diversity of Parasitic Algae Cephaleuros
Basti, Varanasi, Balia and Allahabad districts. Species (Trentepohliaceae, Chlorophyta)
Key words: Trees outside forest, Challenges, Policy, Poplar, In Kerala, India
Plantation forestry.

SII/P-3 Binoy T. Thomas*, M.V. Bhagya and V.P. Thomas

Phycotechnology Laboratory, Department of Botany,
Morphological and Cytogenetical Catholicate College, Pathanamthitta, Kerala, INDIA
Email: bttkripa@gmail.com
Analysis of Sechium edule (Jacq.) Swartz
The genus Cephaleuros comprised of subaerial
1 2
Asikho Kiso *, Sanjay Kumar and Limasenla 1 green algae (Chlorophyta) belonging to the class
Department of Botany, Nagaland University, Lumami-798627,
Ulvophyceae, under the order Trentepohliales and the
INDIA; 2Department of Botany, Banaras Hindu University, family Trentepohliaceae. Leaves of thirty seven host
Varanasi-221005, INDIA Email: mhasikhono@gmail.com, plants were collected from ten different spots of
ksanjay79@gmail.com, limasenla@mail.org Pathanamthitta district, Kerala, India including
agricultural land, forest and plantations. Macroscopic
Sechium edule (Jacq.) Swartz, belongs to the
features of algal thallus were observed under a hand-
family Cucurbitaceae and commonly called as Chayote.
lens and a stereoscopic microscope. Several adequate
The plant chayote is a native of Mexico, but
sections of the infected leaves were taken and identified
considerable diversity is also found in the Indian
using monograph of Thompson and Wujek (1997).Four
subcontinent where it is grown widely in the North-
parasitic species of Cephaleuros were isolated such
East and Southern regions of India. Fourteen Chayote
as C. virescens Kunze & EM Fries (1832: 327), C.
fruit variety were collected from Nagaland, India under
parasiticus Karsten (1891:32), C. diffusus Thomson
the district of Kohima, in order to study their phenotype
& Wujek (1997: 32) &C. microcellularis Y. Suto & S.
and genotype characters. Both the qualitative (shape
Ohtani (2009: 2226). Generally, thallus has a prostrate
of fruit, colour, spines distribution, furrows, ridges,
system and an erect system. Prostrate system is hidden
texture, taste, fibres present or absent), quantitative
in subepidermally or intramatrically. The erect system
traits of Sechium (fruit length, width, area, volume,
is reduced to simple and multicellular setae and 3-8

50 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

celled sporangiophores. As far as C. parasiticus was anti-inflammatory, anti-HIV, anti-oxidant, anti-parasitic,

concerned, C. virescens was widely distributed and anti-tumor, anti-viral, cardio-vascular, chemo-protective,
observed in thirty five host plants. In C. parasiticus, fever, gastrocyte protective, hypocholesterolemia,
leision occurs on both adaxial and abaxial side of the immune stimulating, immune-modulation, metal ion
leaves and causing necrosis of whole leaf tissues of chelating, prebiotic, stomach ulcer and upset,styptic,
leaves, but in the case of C. virescens lesion occurs at whooping cough and wound healing.
adaxial side and necrosis occurs at epidermal and Key words: Anti-cancer, Anti-HIV, Hamirpur region,
palisade cells of leaves. C. parasiticuswas observed Macrofungi, Medicinal properties.
in plants of Lagerstroemia speciosa and Anacardium
occidentale. C. diffusus caused necrosis in the leaves SII/P-6
of Artocarpus incisus and leading to early leaf falling.
C. microcellularis caused lesions on epidermal and Altitudinal Variation in Traits of
palisade leaves of Anacardium occidentale. All Rhododendrons
Cephaleuros species caused either leaf or fruit spot
in a wide range of wild plants and plantation crops of N. Abenthung Kithan*1 , Sanjay Kumar2 and N.S.
economic interest in Kerala, India. The present study Jamir 1
concluded that Cephaleuros species pose direct threat
to the host plant and affect their metabolism. Department of Botany, Nagaland University, Lumami-798627,
Nagaland, INDIA; 2Department of Botany, Banaras Hindu
Key words: Trentepohliales, Tropical, Cephaleuros University, Varanasi-221005, INDIA Email: nabenthung
virescens, C. parasiticus, C. diffusus, C. microcellularis. @gmail.com, ksanjay79@gmail.com, nsjamir@
The quantitative trait variation was studied among
An Enumeration of Medicinal the Rhododendrons of Kohima, Kiphiri (3096, 3112 and
3430 masl) and Wokha at three different altitudes in
Macrofungi from Hamirpur Region of each district of Nagaland. The quantitative traits such
Himachal Pradesh as phenotypic variation (Vp), genotypic variation (Vg),
environmental variation (Ve), variation due to interaction
Jyoti Pathania* and Hem Chander of genotype and environment (Vgxe) with genotypic
Division Botany, Department of Bio-Sciences, Career Point and phenotypic coefficient of variation (GCV and PCV)
University, Hamirpur, H.P., INDIA Email: were recorded. The phenotypic variance was high in
angeltikshu1990@gmail.com, hemchander78@gmail.com leaf breadth (71.7%) supported by the variations due
to environment (86.2%) at an altitude of 3096 of Kiphiri
During the ongoing studies on diversity of
district. The traits such as number of branches and
macrofungi, specimens of macrofungi were randomly
number of inter nodes showed close relationship in GCV
collected from thirteen localities (viz. Chheorin, Doh,
and PCV and suggest less environmental effect. The
Baru, Bohni, Saloni, Bhota, Tikkar- Khattriyan, Kohin,
phenotypic variation leaf breadth was high (66.6%) at
Tikkar-Sujanpur, Doli-Sujanpur, Harson, Ghadalwin and
3112 masl and the Length of peduncle showed close
Fafan) of Hamirpur region of Himachal Pradesh and
relationship between gcv and pcv indicate less
twelve species of medicinal macrofungi viz.
environmental effect on the trait. The altitude 3430 masl
Auricularia auricula judae, Bovista pusilla,
was found not suitable for the variation in leaf breadth
Daldenia concentrica, Ganoderma applanatum,
but more suitable for the variation in peduncal length
Ganoderma lucidum, Lenzites acuta, Phellinus
due to genetic variation (97.9%).The traits with high
gilvus, Pleurotus ostreatus, Schizophyllum
variation may be useful for further investigation into
commune, Trametes pubescens, Trametes versicolor
the Rhododendrons.
and Tyromyces chioneus were identified. These
macrofungi have medicinal utility against/as anti- Key words: Rhododendrons, Altitudes, Phenotypic
dementia, anti-aging, anti-bacterial, anti-cancer, anti- variation, Genotypic variation.
diabetic, anti-fungal, anti-genotoxic, anti-hypertensive,

Abstracts 27-30 November, 2018, NBRI, Lucknow 51

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SII/P-7 adverse conditions of climate and substrate. They are

universally distributed organisms occurring in varied
Propagation and Multiplication of some climatic conditions ranging from the poles to the tropics
Bryophytes under in vitro Condition in earth and look like crust, spreading rapidly over the
surface (crustose lichens) or leafy and loosely attached
Mridula Maurya to the surface (foliose lichens) and branched or shrubby,
hanging from tree twigs or branches, with a single
Botany Department, University of Lucknow, Lucknow-
attachment (fruticose lichens). The lichens are
226007, U.P., India, Email: mridulamaurya.mailbox@gmail.com
considered valuable bio-monitors of environmental
Bryophytes are earlier land plants and are well change and exhibit sensitivity to the air pollution. Udala
known as primary colonizers on bare land. They community development area (21° 34' 37.7832'’ N 86°
commonly grow in moist and humid places under shady 33' 55.1124'’ E), in the Mayurbhanj district of Odisha
condition but they are often associated with disturbed is endowed with rich forest cover and home to many
habitat, barren rock surface under extreme climatic tribes and enriched with variety of tree species such
conditions and mineral enrichment. Hence they play as Shorea robusta, Pterocarpus marsupium,
large role in habitat formation for secondary colonist. Buchanania lanzan, Azadirachta indica,
They also serve as indicator of environmental pollution Phyllanthus emblica, Mangifera indica, Madhuca
due to their simple thallus composition. Here studies indica, Terminalia arjuna, Terminalia bellerica,
have been done on propagation and multiplication of Syzygium cumini, Pongamia pinnata, Ficus sp.
members of aytoniaceae family due to their unique Shorea robusta dominates in all forest in the region.
properties (medicinal value/ interesting chemical An extensive exploration was conducted to find out
compounds having medicinal value, insect repellant/ the diversity of lichens in Udala community development
antibacterial and antifungal, biogeoindicator, pollution area during 2017-18. Over 219 specimens of lichens
indicator, (169-foliose, 50-crustose) were collected from the bark
Key words: Bryophytes, Environmental pollution, of trees in the study area and were identified as 22
Propagation, Multiplication, Aytoniaceae. species under 15 genera and 11 families. The
dominating genera were Dirinaria, Parmotrema,
SII/P-8 Diorygma, Letrouitia, Pyrenula and Graphis, while
Graphidaceae, Parmeliaceae, Physciaceae,
Lichen Diversity of Udala Area in Teloschistaceae, Letrouitiaceae were dominating
Mayurbhanj District of Odisha, India families. The findings of the present study revealed
that lichen diversity is rich in this area and needs further
Srimay Pradhan 1 *, Prashant Kumar Behera 2 , study to conserve these lower plants.
Sandeep Kumar Nayak3 and Kunja Bihari Satapathy1 Key words: Lichen, Udala, Mayurbhanj, Odisha.
Post Graduation Department of Botany, School of Applied
Sciences, Centurion University of Technology and SII/P-9
Management, Bhubaneswar, INDIA; 2Lichenology
Laboratory, CSIR-National Botanical Research Institute, A Preliminary Investigation on Mosses
Lucknow, INDIA; 3Post Graduation Department of Botany, of Nagaland (North-East India)
Utkal University, Bhubaneswar, INDIA
Email: srimay.pradhan@cutm.ac.in, bkprashant10@ Vinay Sahu* and A.K. Asthana
gmail.com, sandeepnayak75@gmail.com, kbsbotuu@ Bryology Laboratory, CSIR-National Botanical Research
gmail.com Institute, Lucknow-226001, INDIA
Lichens are Thallophytes which comprise a Email: sahuvinay8@gmail.com, drakasthana@rediffmail.com
unique group of plant consisting two unrelated
organisms, a fungus and an alga, growing together in a The present study deals with the investigation of
close symbiotic association and can grow in most moss taxa of Nagaland. This state is covered by hill
ranges and is very rich in medicinal and economically

52 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

important plants. During the present study investigation IAA+BAP+AC combination proved optimal for early
on bryophytes collected from Dzukou valley, Saramati rhizogenesis and seedling development. The
Hills and Thanamir have been carried out. It revealed morphogenetic response of pseudobulb explants were
the occurrence of 99 species of mosses belonging to varied depending upon in vivo grown plants and in
52 genera of 26 families. Among the investigated vitro raised plantlets. In vivo explants induced
families in mosses, Bryaceae, Dicranaceae and regeneration response via direct PLB formation at BAP
Pottiaceae seem to be more dominant in the region, 3 mg l-1. Interestingly, TDZ (3 mg l-1) supplemented
followed by Meteoriaceae and Polytrichaceae. Genus with AC (2 g l-1) and IAA (2 mg l-1) + TDZ (3 mg l-1)
Pogonatum and Pohlia have maximum 6 number of + AC (2 g l-1) proved optimal for regeneration, culture
species followed by Genus Bryum and Dicranum with multiplication and healthy plantlet formation in the in
4 species. Atrichum crispulum Schimp ex Besch. has vivo and in vitro explants respectively. Synthetic seed
been recorded as new to India, while 54 species of technology is today avital tool for the in vitro
mosses have been reported for the first time from propagation and conservation of plants. Protocorm like
Nagaland. bodies (PLBs) formed in vitro were encapsulated in
Key words: Nagaland, Moss, North-East India. 3% sodium alginate matrix to form synseeds.
Encapsulation ensures slow growth and long term
SII/P-10 preservation. The effect of storage at 4oC and 25oC
was tested on the conversion frequency of seeds at 15
In vitro Seed Germination, Regeneration days intervals. Their multiplicity and conversion
frequency was markedly impaired upon storage. The
and Synthetic Seed Production Based protocol developed in the present investigation can be
Micropropagation of a Terrestrial commercially exploited and used as a means for the
Medicinal Orchid (Malaxis acuminata rapid propagation and conservation.
D. Don) Key words: Pseudobulb, Mitra medium, Micropropagation,
Protocorm like bodies, Orchid.
Anuprabha1* and Promila Pathak2
GGDSD College, Sector-32, Chandigarh-160032, INDIA;
Orchid Laboratory, Department of Botany, Panjab A Preliminary Study on the Relative
University, Chandigarh-160014, INDIA, Email:
anu857pu@gmail.com, ppathak2007@gmail.com Abundance and Diversity of Insect
Malaxis acuminata is a rare, terrestrial, Pollinators in Different Fruit Orchards
pseudobulbous, shade loving, and medicinal orchid of of Kumaun Himalaya, India
Himalayan region. It is used as aphrodisiac, febrifuge
and has a cooling effect. Commercially known as Fasuil Farooq* and Manoj Kumar Arya
‘Jeevak’, its dried pseudobulbs from a constituent of Insect Biodiversity Laboratory, Department of Zoology, DSB
‘Ashtavarga’ drugs used in the preparation of ayurvedic Campus, Kumaun University, Nainital-263002, Uttarakhand,
tonic ‘Chyavanprash’. Its natural populations are INDIA, Email: faisalmalik.jk@gmail.com
decreasing mainly due to habitat destruction and
commercial collection pressures, so several efforts have Pollinators are regarded as important keys for
been made to develop an efficient micropropagation the production of fruits to increase farmers income.
system. Presently the immature seeds (6, 10, 14 wap) The present study aims to study the relative abundance
were inoculated on M medium with plant growth and diversity of insect pollinators in three fruit orchards,
regulators [IAA (1 mg l-1) and BAP (1 mg l-1)], organic i.e. apple, peach and apricot orchards, respectively.
supplements [YE (2 g l -1 )] and AC (2 g l -1 ). Scan sampling method was used to explore the diversity
Combination containing IAA supplemented with AC of insect pollinators. Observations of the insect
proved optimal for early germination and rapid pollinators were conducted from 8.00 to 11.00 AM and
protocorm multiplication; media containing 2.00 to 4.00 PM in sunny days. A total of 563 individuals

Abstracts 27-30 November, 2018, NBRI, Lucknow 53

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

were collected, belong to 47 species in four order as one of the biodiversity hotspots. Himalayan
(Hymenoptera, Diptera, Lepidoptera and Coleoptera). grasslands have been classified into five types viz.,
Number of species of insect pollinators found in apple warm temperate grasslands, cool temperate grassy
orchard (31 species) and peach orchard (22 species) slopes, sub alpine meadows and thatches, alpine
were higher than that in apricot orchard (13 species). meadows and steppe formations of the trans-
Insect pollinators in all the three fruit orchards were Himalayas. Grasses have significance in human life
dominated by giant honey bee (Apis dorsata) followed far beyond meeting the basic requirements of food and
Apis cerana and Bombus haermorrhoidalis. Higher fodder. Economic uses include their use as raw material
foraging activities of insect pollinators occurred in the of sugar and beverages (the sugarcane), as constituent
morning. of baking powder, pies and puddings, building materials
Key words: Abundance, Diversity, Fruits, Insects, Species. for huts and tree homes (machons), art and craft,
vaulting poles (bamboos and reed grasses), paper,
SII/P-12 sweeping brooms (broom grasses) and finishing and
stiffening material in textiles (corn starch). Several
Ecology and Economy of Grasses of grasses are sown as lead plants to reclaim the unused
lands like mud flats and tidal estuaries (eg. cord grass)
Trans-Himalayan Region and alkaline and saline lands. The present study reports
the ecological and economic importance of grasses of
Mudassir Ahmad Bhat*, Priya Badgal, Poonam
Trans-Himalayan region. The grasses for which data
Chowdhary and Amarjit Singh Soodan
on the economic and ecological significance has been
Department of Botanical and Environmental Sciences, Guru presented in the paper include Piptatherum gracile
Nanak Dev University, Amritsar-143005, Punjab, INDIA Mez, Piptatherum munroi (Stapf) Mez, Stipa brandisii
Email: mabhat90@gmail.com Mez, Brachypodium sylvaticum (Huds.) P. Beauv.,
Poaceae constitutes the fifth largest angiosperm Koeleria macrantha (Ledeb.) Schult., Trisetum
family as far as the number of species is concerned. aeneum (Hook.f.) R. R. Stewart, Agrostispilosula
Within monocotyledons, the family stands at the second Trin., Calamagrostis holciformis Jaub. & Spach.,
rank after Orchidaceae and includes more than 11000 Calamagrostis pseudophragmites(Haller f.) Koeler,
grass species distributed in 700-800 genera. India is a Muhlenbergia huegelii Trin, Deschampsia
megabiodiversity country that harbours a rich diversity cespitosa(L.) P. Beauv., Dactylis glomerata L.,
of grasses. Poaceae has emerged as the largest plant Poaalpina L. Eremopoaaltaica (Trin.) Rozhev.,
family in the country with a representation of 268 genera Alopecurus himalaicus Hook. f., etc. and
and 1300 species. Ever since their origin and economically important include Koeleria litvinowii
diversification, grasses have evolved a number of biotic Domin, Trisetum spicatum (L.) K. Richt., Festuca
and abiotic interactions that have made them an pratensis Huds., Lolium perenne L., Poa albertii
important component in almost all ecosystems but Regel, Poa pratensis L., Alopecurus arundinaceus
dominant elements in the grasslands of the world. Apart Poir., Phleum alpinum L., Bromus catharticusVahl,
from monitoring, grasses have been found to possess Bromus inermis Leyss, Elymus nutans Griseb., Elymus
tremendous potential to maintain the carbon balance dahuricus Turcz. Ex Griseb., Bromus japonicas
and mitigating the damage to the environment. Several Houtt., Hordeum brevisubulatum (Trin.) Link,
grass species have been identified and recommended Pennisetum orientale Rich., Setaria italic (L.) P.
as bioenergy crops to obtain bio-fuels that would reduce Beauv. and Tenaxia cumminsii (Hook. f.) N.P. Barker
the burning of fossil fuels and release of Green House & H.P. Linder.
Gases (GHG’s) into the atmosphere. Within the country, Key worlds: Poaceae, Grassland, Himalayas.
north western Himalayan region has been recognized

54 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SII/P-13 Key words: Cytogenetics, Electrophoresis, Bauhinia, Potent,

Investigation, Variations.
Characterization of Most Potent Species
of Bauhinia using Gel Electrophoresis
Studies on Plant Resources in Gunjan
Kumari Nutan Sinha*, Tanuja Singh and Jyoti
Kumari Ecological Park, a Freshwater Pitlake
Department of Botany and Biotechnology, Thakur Prasad in Raniganj Coal Field Area, West Bengal
Singh College, Patna-800001, Bihar, INDIA, Email: India: Implications for Sustainable Use
To trace the interrelationships and for Pinaki Chattopadhyay1*, Saikat Mondal2, Pallavi
identification and characterization of diversity among Chattopadhyay3 and Debnath Palit1
four species of medicinally most potent plants, 1
Department of Botany, Durgapur Government College,
Bauhinia viz. Bauhinia acuminata, Bauhinia Durgapur, West Bengal, INDIA; 2Department of Zoology,
purpurea, Bauhinia racemosa and Bauhinia Raghunathpur College, Purulia, West Bengal, INDIA;
variegata belonging to sub-family Caesalpiniaceae, Department of Microbiology, Amity University, Lucknow
cytological study with respect to chromosome number, campus, Lucknow District, Uttar Pradesh, INDIA, Email:
somatic chromosome length of component arms of iampinu7@gmail.com, sairaniganj@gmail.com, pallavi96
chromosome, T. F% and chromosome type variation chatto@gmail.com, drdpalit@gmail.com
were investigated. Results showed that the three species Pitlakes are formed as a result of water deposition
have chromosome number 2n=28, the value of the total or storage in a huge pit which has been created due to
chromatin lengths was lowest (53.48µ) in Bauhinia open cast mining. Pitlakes are storage of huge amount
racemosa and highest (57.32µ) in Bauhinia purpurea of water which indirectly and directly supports
while in Bauhinia acuminata this value was 56.78µ ecological succession by sustaining many flora and
suggesting a close relationship between Bauhinia fauna nearby. The present study is an outcome of the
purpurea and Bauhinia acuminata. The T.F% values exploration of flora at embankments and adjoining areas
of Bauhinia acuminate and Bauhinia purpurea stand of pitlake of Gunjan ecological park, a 30 years old
close to each other. Seed storage Protein profiles of freshwater pitlake in Raniganj Coal Field Area of
four species of Bauhinia were analysed by sodium Asansol subdivision, Paschim Burdwan District, West
dodecyl sulphate polyacrylamide gel electrophoresis Bengal, India. In the present treatise we have
(SDS-PAGE). Total seed storage protein of Bauhinia enumerated 210 species of angiosperms, 12 species of
species resolved on 10% SDS Polyarylamide gels pteridophytes, 5 species of bryophytes, 9 species of
showed variations in their banding pattern. Results of algae, 70 species of macro fungi, 2 species of slime
SDS-PAGE pattern of a few protein bands were up moulds and a species of crustose lichen. The plant
regulated whereas some other bands showed down species were identified using standard literature. This
regulation. Beside the common bands among the studied pit lake is enriched with floral assets like medicinal
taxa, 7.37, 31.85, 41.56, 54.854 and 261.143 kDa plants, vegetables, fruits and some edible mushrooms
proteins were found to be common in B. acuminata and this pit lake based ecological park promoting
and B. purpurea and 261.143 kDa protein was found ecotourism also. There is immense opportunity to carry
common in all the four species. Maximum genetic out further research work particularly for exploration
affinities were observed between B. acuminata, B. of plant resources in this pit lake. Judicious and rational
purpurea (45.45%), while minimum between and B. exploitation of plant resources in a sustainable way can
racemosa and B. variegatea (20%). On the molecular conserve biodiversity, maintained ecological balance
level, the present study gave the results with wide and also enhance socio-economic up liftment of local
variations in their band numbers. Maximum number of stakeholder.
protein bands (11 bands) was observed in B. racemosa, Key words: Pitlake, Plant diversity, Gunjan Ecological Park,
while minimum (7 bands) in B. purpurea and B. Ecotourism, Floral assets.

Abstracts 27-30 November, 2018, NBRI, Lucknow 55

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SII/P-15 Lichens are considered as one of the best

material for carrying out environmental pollution
Assessment of Molecular Diversity of monitoring and climate change. Due to their higher
Ribes grossularia using RAPD Markers sensitivity against microclimate changes, lichen
communities quickly predict the climate and
Diksha Molpa, Pooja Saklani*, Pawan Singh Rana environmental changes in a particular area within short
and Chandresh Chandel duration of one or two decades, while higher plant
require more time. The lack of vascular and root system
HNBGU (A Central University), Srinagar-246174,
and dependency to absorb water and nutrients passively
Uttarakhand, INDIA, Email: poojasaklani@rediffmail.com
from their environment make it sensitive against
Ribes grossularia is also known as ‘Himalayan environmental perturbation. The potential areas for
Currant’ mainly found in open rocky slopes at altitudes carrying out climate change studies based on the
of 2400-3300 m. It has low tocopherol content and high availability of herbarium records in India belongs to a
percentage of gamma-tocopherol. It has anti- number of localities in Indian Himalayan regions (IHRs),
inflammatory and anti-tumoral effects. In the present such as Jammu & Kashmir, Himachal Pradesh,
study, leaves of plant were collected from ten locations Uttarakhand, Sikkim, Arunachal Pradesh and hill areas
of Uttarakhand. Twenty RAPD primers from Operon of Assam and Darjeeling district of West Bengal. These
series (OPA 1-20) were screened for the finger printing areas were well explored for their lichen specimens in
but only three primers OPA-04, OPA-10 and OPA-12 the past and the specimens from these localities are
gave satisfactory results. The primer amplified DNA preserved in different Indian herbaria can be utilized
products from each location generated significant band for such studies. From the lichen herbarium records
patterns. On the basis of band pattern produced by the foot hills of Darjeeling in eastern Himalaya
each primer, the phylogenetic tree comprising of total represents, 151 species of lichen belonging to 61 genera
ten accessions of R. grossularia was constructed by of 29 families from 11 localities. The same localities
UPGMA. In the dendrogram, the accessions were revisited after a gap of 48 years revealed the
divided into two major cluster groups i.e cluster 1 and occurrence of only 126 species belonging to 45 genera
cluster 2 based on the genetic variability. Among both of 22 families, out of which only 26 species were
clusters second major cluster was found more diverse. common in both past and present study while 100
The genetic variability study for the R. grossularia species found in the recent survey were entirely
species is not only valuable for the conservation of their different species reported in the year 1966. The present
germplsam but would also be useful for breeding new survey revealed a significant change in growth form,
or novel varieties. habitat and community structure of lichens as compared
Key words: Ribes grossularia, RAPD, UPGMA. to past. The lichen herbarium specimen records, not
only helps in reconstructing historical trend in
SII/P-16 atmospheric deposition but also exhibit the temporal
variation in the community structure of lichens due to
Lichen Communities and Species changes in climate. The observation in shift of lichen
Indicate Climate Thresholds in Indian communities/species in response to climatic conditions
and environmental pollutants warrant its use as a model
Himalayas to study the global climate and/or local microclimate
Rajesh Bajpai* and D.K. Upreti
Key words: Lichens, Climate change, Herbarium records, Air
Lichenology Laboratory, Plant Diversity Systematics and pollutants.
Herbarium Division, CSIR-National Botanical Research
Institute, Rana Ptatap Marg, Lucknow-226001, INDIA, Email:

56 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SII/P-17 possibly due to their habitat preservation over the time.

Interestingly, nearly ten species of mosses family
Assessment of Bryophyte Diversity at Pottiaceae and four species of liverwort genus
Tiger Hill (Darjeeling, Eastern Himalaya) Jungermannia L. have been recorded in the recent
surveys which were previously not documented from
and its Importance in Context of this site. These bryophytes are known to be less habitat
Anthropogenic Activities specific and well adapted to the harsh environmental
conditions, therefore their presence at Tiger Hill
Reesa Gupta* and A.K. Asthana suggests that the bryophyte flora here has undergone
Bryology Laboratory, CSIR-National Botanical Research changes owing to continuous anthropogenic
Institute, Lucknow-226001, INDIA, Email: guptareesa44@ disturbances and habitat modifications.
gmail.com; drakasthana@rediffmail.com
Key words: Darjeeling, Bryophytes, Diversity.
Bryophyte diversity of Tiger Hill (Darjeeling,
Eastern Himalaya) has been elucidated and
documented in the present study. This place is
celebrated as the highest point of Darjeeling city and is
a well-known tourist place of the region. A total of 48
Current Taxonomic Status of
species under 36 genera and 22 families of bryophytes Caesalpinioideae-Leguminosae in
are being reported presently. Mosses were more Uttar Pradesh
abundant than liverworts and hornworts. The bryophyte
distribution was studied across six selected habitats. Shivaraman Pandey 1 *, Gauri Saxena 2 ,
The overall trend in the bryophyte distribution here Rameshwar Prasad1 and Lal Babu Chaudhary1
suggests that maximum number of bryophytes were 1
Plant Diversity, Systematics and Herbarium Division, CSIR-
present on wet rocks whereas tree barks also showed
National Botanical Research Institute, Rana Pratap Marg,
substantial bryophyte distribution. The trend of Similarity Lucknow-226001, INDIA; 2Department of Botany, Lucknow
Indices calculated for different habitats indicates University, Lucknow, INDIA, Email: pandeyshivaraman@
towards rich diversity of bryophytes. At Tiger Hill gmail.com, gaurigupta72@yahoo.com, rameshwar.nbri@
bryophytes inhabiting soil covered rocks and wet rocks gmail.com,dr_lbchaudhary@rediffmail.com
were more similar in species composition whereas, soil
Leguminosae (nom. alt. Fabaceae) is the third
and corticolous habitats showed least similar bryophyte
largest family of flowering plants after Orchidaceae
flora as compared to other habitats. The site witnesses
and Asteraceae with approximately 750 genera and
substantial anthropogenic activities that include
19,400 species in the world. It is distributed throughout
vehicular movement, construction work, clearing of
the world in almost all habitats ranging from wetlands
forest area for space creation, manmade pollutants such
to dry and cold deserts, from tropical forests to alpine
as waste and garbage and regular influx of people.
habitats and from sea level to 7000 m in the Himalaya.
Some species of bryophytes which were present nearly
According to recent classification, the family has been
50 years ago and have been re-collected from this site
divided into six subfamilies: Cercidoideae, Detarioideae,
indicate that these are very well adapted to the habitat
Duparquetioideae, Dialioideae, Caesalpinioideae and
and abiotic conditions of this area. Further some taxa
Papilionoideae. The old subfamily Mimosoideae has
such as Porella plumosa (Mitt.) Parihar,
been placed under Caesalpinioideae as a separate
Crysocladium horridum Dixon, Symphyodon
clade. In the present study, the taxonomy and diversity
oblongifolius (Ren. et. Card.), Rhynchostegium
of the subfamily Caesalpinioideae found in Uttar
celebicum (Lac.) A. Jeager etc. were present at Tiger
Pradesh have been dealt elaborately based on extensive
Hill nearly five decades ago but have not been recorded
field and herbarium studies. Caesalpinioideae is
in recent times. On the other hand, very few species
represented by about 2251 species under 171 genera
such as Plagiochila sciophila Nees, and
across the world. In India, it is represented by about
Cyathophorella hookeriana (Griff.) Fleisch. have
169 species under 31 genera and largely distributed in
been retained here through the span of five decades,

Abstracts 27-30 November, 2018, NBRI, Lucknow 57

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

the moist tropics. The present study revealed 45 species (H’=3.325) and large (H’=3.185) homegardens. Tree
under 12 genera in Caesalpinioideae from Uttar density was found to be higher in the large homegardens
Pradesh, including 13 cultivated species. Among (249 trees ha-1) and followed by medium (216 trees
different genera, Senna is the dominant ones with 12 ha-1) and small homegardens (195 trees ha-1). The study
species which constitutes about 26% of total shows that vegetables are the major component of the
Caesalpinioideae occurring in Uttar Pradesh. The homegardens followed by fruit, medicinal, firewood and
genera Bauhinia and Caesalpinia hold second and ornamental plants. The households recorded most
third position with 9 (20%) and 6 (13%) species species as useful for vegetable (24%) followed by fruit
respectively in the state. Out of 45 species, 10 species (18%), firewood (12%), medicine (11%), ornamental
are commonly occurring throughout the state in (9.8%), fodder (9.2%), timber (6.7%) and others
different biogeographic regions like Terai Region, (9.3%). Higher values of biomass were recorded in
Gangetic Plain, Vindhyan Region and Semiarid Region. small followed by medium and large homegardens, and
While, some of the species are area specific, that occurs thus biomass carbon stock amongst the homegardens
only in very restricted pockets. Each species has been was in the order: small (122.3 Mg C ha-1) > medium
dealt with detailed taxonomic information such as (109.0 Mg C ha-1) > large (90.6 Mg C ha-1). Soil organic
correct nomenclature, description, distribution, carbon (SOC) stock upto 1 m soil depth was also
phenology and line drawing illustrations or photographs. recorded maximum in small (229.5 Mg C ha-1) followed
Key words: Caesalpinioideae, Diversity, Uttar Pradesh, by medium (176.2 Mg C ha-1) and large (144.6 Mg C
Taxonomy. ha -1 ) homegardens. The study results indicate that
traditional homegarden agroforestry systems mimics
SII/P-19 forest ecosystems and thus qualify as potential carbon
sinks through sustainable management practices.
Biodiversity Conservation and Ecosystem Key words: Homegardens, Species diversity, Biomass carbon
Carbon Storage in Traditional stock, SOC stock
Homegardens in Mizoram, Northeast SII/P-20
Algal Diversity with Special Reference
Soibam Lanabir Singh and Uttam Kumar Sahoo* to Chlorophyceae of Shershah Suri Pond,
Department of Forestry, School of Earth Sciences and Natural Kanpur, U.P.
Resource Management, Mizoram University, Aizawl-796004,
Mizoram, INDIA, Email: uksahoo_2003@rediffmail.com,
Vishal Saxena* and R.K. Khare
Department of Botany, Govt. Model Science College (Jiwaji
The study was conducted to document species University), Gwalior, M.P., INDIA, Email:vishsaxena@
composition, their utilization and carbon storage in rediffmail.com, rakesh.khare1965@ gmail.com
traditionally practiced homegardens in Mizoram,
Northeast India. A stratified sample of 42 homegardens Fresh water is natural source of fundamental
classified on the basis of size as small (0.02 to 0.05 importance to all living beings on earth. Natural water
ha), medium (0.05 to 0.75 ha) and large (> 0.75 ha) is rich in bacteria, algae, protozoa, worms and other
with 14 homegardens each were surveyed in 2017-18. organisms. Algae, carbon fixing and oxygenating
A total of 198 species (82 trees, 31 shrubs and 79 herbs) organism, and their diversity is the key parameter to
belonging to 69 families and 169 genera were recorded, determine the productive nature of the pond ecosystem.
being most of them perennials (78 %). Overall highest The limnological survey and the algal diversity of
plant species was recorded in small (157 species) Shershah Suri freshwater pond in Kanpur (26o 24’29"
followed by medium (141 species) and large (141 N latitude and 80o 23’34’E longitude) district, Kanpur
species) homegardens. Diversity index for trees was Nagar (U.P.) was undertaken. Water samples were
maximum in medium (H’=3.780) followed by small analysed for the concentration of various physico-
chemical parameters. Phytoplankton of all the sides of

58 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

pond were collected for analysis. Detailed examination of Selinum also fall under the Catalogue of Life list by
shows that algae exhibit a high degree of qualitative Catalogue of Life Indexing. Since the plant has
and quantitative variance throughout stretch of principal constituents (alkaloids as well as essential oils)
Shershah Suri Pond. The aquatic system have shown mainly in the root, the plant is completely destroyed
existences of 34 algal forms and out of them 12 belongs while being put to use. To avoid mass destruction of
cyanobacteria, 4 to Euglenophyceae, 7 to this high altitude medicinal plant, several conservation
Bacillariophyceae and 11 to chlorophyceae. There are strategies need to be under taken such as cultivation,
certain algae occur in all the season. The present paper germplasm conservation, seed bank procurement etc.
deals with the study of members of chlorophyceae e.g. Conservation of plants can be tackled by scientific
Cladophora, Oedogonium, Spirogyra, Vaucheria and techniques such as in situ conservation to secure
Zygnema. genetic variation and also conserve population of
Key words: Algal diversity, Chlorophyceae, Aquatic system, Selinum; ex situ conservation or biodiversity
Shershah Suri Pond. management for human utilization; local action such
as Legislation, to give sustainable benefit to the present
SII/P-21 generation while retaining its potential to meet the
requirements and aspirations of future generations as
Conservation Strategies in Selinum: well.
A Himalayan Medicinal and Aromatic Key words: Conservation, Threatened, Medicinal, Bhutkeshi,
Selinum, Apiaceae.
Ravi Prakash Srivastava* and Gauri Saxena
Department of Botany, University of Lucknow, Lucknow- A Preliminary Diversity Analysis of
226007, INDIA, Email: ravisrivastava02@gamil.com, Dicotyledonous Plants of Dima Hasao
gaurigupta72@ yahoo.com
District of Assam, India
Medicinal plants and herbal medicines form an
important part of the treatment in the Indian medicine R.K. Verma1*, B.A. Khan1, V.V. Wagh1, Baleshwar1,
systems. One such Himalayan medicinal herb is S.K. Behera1, S. Tamta2 and L.B. Chaudhary1
Selinum that belongs to Apiaceae family. The plants
are also widely distributed over the temperate zones Plant Diversity, Systematics and Herbarium Division, CSIR-
of both the hemispheres. The genus commonly referred National Botanical Research Institute, Lucknow-226001,
INDIA; 2Department of Botany, DSB Campus Kumaun
to as Bhutkeshi in India, is known for its medicinal and
University, Nainital-263001, Uttarakhand, INDIA, Email:
aromatic properties and has been used as an alternative dr_lbchaudhary@rediffmail.com
source and adulterant of compounds obtained from
Nardostachys jatamansi. All the plant parts including The study enumerates the dicotyledonous plants
leaf, stem and root are utilized for medicinal uses such found in the district Dima Hasao in Assam with their
as nervine sedative in hypertension, aphrodisiac with important taxonomic information. This is the outcome
analgesic properties and in common ailments like cold, of the several botanical tours conducted to different
cough, fever, wounds, stomachache and toothache etc. parts of the district during 2017–2018. Dima Hasao
It is also used for aromatic purposes such as incense. district, formerly known as North Cachar Hills district,
These plants have been locally as well as globally is located in the southern part of Assam between 240
exploited that is why the current status of plant is now 582 to 25o472 N latitudes and 92o272 to 93o432 E
near threatened. According to the IUCN Red List 2001, longitudes at elevations ranging from 140 to 1866 m.
the species Selinum carvifolia, was designated as The entire district comprises about 89 % forest cover
endangered in the United Kingdom. Furthermore, of its total geographical area of 4,888 sq. km. with
another Red List by the IUCN in 2009 indicated evergreen, semi-evergreen and deciduous types of
Selinum vaginatum as threatened species and was forest. The Jhoom cultivation is the major agricultural
considered to be in low risk category. Several species practice in the area while the settled agricultural land

Abstracts 27-30 November, 2018, NBRI, Lucknow 59

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

is only 1.29 % of the total geographical area of the pharmacological aspects, ecology, growth condition and
district. After conducting several tours, about 436 environment needed; products and by-products,
species belonging to 307 genera and 87 families have international and local market and current scenario
so far been collected and identified from the study area. about demand and supply, current issues and challenges
All voucher specimens have been deposited in CSIR- for conservation, domestication and commercial
National Botanical Research Institute, Lucknow cultivation, future prospect and recommendations and
(LWG). Based on life forms 184 species have been need of policy support etc.
identified in herbs, 70 in shrubs, 146 species in trees Key words: Pedalium murex, Bada Gokhru, Large Caltrops,
and 39 species in climbers. Among all families, Medicinal plant.
Fabaceae with 92 species, and among genera,
Crotalaria with 9 species were found largest in the SII/P-24
study area. Some of the other dominant families are
Asteraceae (34 spp.), Euphorbiaceae (21 spp.), Checklist of Green Algae (Chlorophyceae)
Amaranthaceae (20 spp.), Malvaceae (14 spp.), of Uttar Pradesh, India
Acanthaceae (12 spp.) and Convolvulaceae (10 spp.)
Similarly, Ficus (8 spp.), Desmodium (6 spp.) and Sushma Verma* Kiran Toppo and Sanjeeva Nayaka
Senna (5 spp.) are frequently occurring genera. One
of the interesting plants Nepenthes khasiana has also Algology Laboratory, CSIR-National Botanical Research
Institute, Lucknow-226001, INDIA, Email: creation.sanju@
been relocated from the area. During the present study,
about 47 highly medicinally important and useful plants
were also recorded from the study area based on the Algae are predominantly aquatic photosynthetic
information gathered by locals. organisms and are the major primary producers of
freshwater ecosystem. The class chlorophyceae are
Key words: Diversity, Dima Hasao District, Dicotyledon,
Assam. diverse group of green algae comprising of various
forms distributed widely in freshwater bodies and
SII/P-23 terrestrial habitats. They include some of the most
common species as well as several members that are
Pedalium murex L. from Wild to ecologically significant. Thallus organization of this
group varies from microscopic unicellular to
CommercialProduction:An Understanding
multicellular macroscopic forms, may be colonial,
with Issues and Challenges coccoid, palmelloid, sarcinoid, syphonacious,
filamentous, thalloid and or pseudoparenchymatus. In
Sujata Shekhar*, Akhilesh Kumar and J.N. India the total algal taxa are 7396, with class
Shrivastava chlorophyceae representing approximately 2592 taxa.
Department of Botany, Dayalbagh Educational Institute The present checklist of class chlorophyceae is the
(Deemed University), Agra-282005, Uttar Pradesh, INDIA first compilation of the freshwater algal flora of Uttar
Email: akhilpbh@rediffmail.com Pradesh based on the updated literature records and
Pedalium murex (Pedaliaceae), commonly bibliographic data intending to contribute towards the
known as Large Caltrops in English and Bada Gokharu knowledge and information on the algal biodiversity of
in Hindi. It is a mucilaginous medicinal herb having the state. The list summarizes the bibliographic database
multiple uses in traditional medicine system. It is mainly from approximately 120 scientific research papers, 6
used to cure reproductive disorders, like impotency in books and 20 theses from libraries of various Research
men, nocturnal emissions, gonorrhea as well as Institutes and Universities in Uttar Pradesh. The
leucorrhoea in women. It is also useful in the treatment taxonomic identity of the taxa is checked by
of urinary and gastrointestinal tract disorders. The www.algaebase.org designed by M. Guiry. The present
present paper deals with botanical description, origin, list records 973 taxa of class chlorophyceae pertaining
diffusion and distribution, usages in traditional and to 9 orders, 35 families and 115 genera from 24
modern system of medicine, phytochemical and different aquatic and terrestrial habitats of

60 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

approximately 65 districts of Uttar Pradesh. The genus reticulate, foveolate, reticulately-rugulate, reticulately-
Cosmarium was recorded to be the dominant taxa with foveolate, stellate papilllate, irregularly thickened cell
approximately 142 species followed by the genus walls, irregularly ribbed to faintly tuberculate. Although,
Oedogonium with approximately 100 species. The these characters may not be utilized to classify the
least number of taxa recorded are from the genus genus at sungeneric or sectional, but definitely they
Papenfussiomonas, Trochiscia, Sphaeroplea and add an additional features to the individual taxon, which
Protosiphon represented with single species each. The tremendously help in the identification.
present study could be considered as a baseline data Key words: Diversity, Seeds, SEM, Identification, Astragalus.
for further exploration on the algal biodiversity from
the state of Uttar Pradesh. SII/P-26
Key words: Green algae, Freshwater Chlorophyceae,
Checklist, Uttar Pradesh Distribution and Diversity of Lichens
in Dima Hasao district, Assam with
Special Reference to Forest Health
Application of Macro and Micromor-
Gaurav K. Mishra, Sanjeeva Nayaka and Dalip
phological Characters of Seeds in Kumar Upreti
Diversity Analysis of Astragalus L. Lichenology Laboratory, CSIR-National Botanical Research
(Legumonosae) in India Institute, Rana Pratap Marg, Lucknow-226001, U.P., INDIA,
Email: gmishrak@gmail.com
Shivani Kashyap and L.B. Chaudhary The Indian Himalayan region exhibit rich diversity
Plant Diversity, Systematics and herbarium Division, CSIR- of lichens in India. The Western Himalayan region has
national Botanical Resarch Institute, Lucknow-226001, luxuriance of lichens due to its unique topography and
INDIA, Email: dr_lbchaudhary@rediffmail.com climatic condition, however, the Eastern Himalayan
Astragalus L. is the largest angiospermic genus region showed rich diversity of lichens due to its warmer
in the world with about 2900 species. In India the genus and moist climate condition. The rich moist and warmer
which is chiefly found in the Himalaya, is represented climate in Eastern Himalayan region supports forest
by about 79 species. Due to its large size and enormous with dominance of evergreen tree vegetation. The
morphological plasticity, the taxonomy of the genus is Dima Hasao district of Assam is situated at higher
very complex and challenging. The identification of altitudes between 500 to1500 m having a suitable
species only based on macro morphological characters condition for many lichen taxa belonging to family
is tedious in the genus. In the present study, therefore, Graphidaceae, Pertusariaceae, Pyrenulaceaeand
both macro and micro morphological features of seeds Thelotremataceaeas the micro lichens together with
like shape, size, colour and spermoderm pattern under macro-lichens families such as Parmeliaceae,
Scanning Electron Microscope (SEM) have been Physciaceae, Collomataceae and Cladoniaceae. The
examined to evaluate their role in the identification and district is represented by occurrence of 125 species
classification. The seeds of 30 species belonging to belonging to 54 genera and 16 families. Among the
about 14 sections have been analysed for the study of different genera of lichens, Graphis showed its
variations among them in the present study. The overall maximum diversity represented by 20 species followed
size of the seeds ranges from 1.5-3.2 x 0.8-2.2 mm. by lichen genera Parmotrema and Pyrenula with 10
The shape of the seeds, observed in the investigated and 9 species respectively. The study area has
species, are reniform, ovoid, oblong, discoid, dominance of crustose lichens represented by 91
suborbicular and deltoid. In the majority of the cases species followed by 27 species of foliose lichens. Based
the seeds are brown or blackis-brown. The on the occurrence of lichen bio-indictor communities
spermoderm pattern showed great variability in the study area can be mapped in to different zones
ornamentations which exhibit variation from rugulate, having different levels of environmental conditions and
forest health. The Lobariantaxa considered as sensitive

Abstracts 27-30 November, 2018, NBRI, Lucknow 61

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

to micro climatic condition and air quality are reliable well as Bacteria. Fungi isolated were mostly from the
indicators of species rich old forest with long forest group Basidiomycetes, Ascomycetes, Deuteromycetes
continuity. The rich diversity of all the growth forms of and Phycomycetes. Bacterial isolates include
lichens clearly indicates that moist of the forest within Staphylococcus sp., Streptococcus sp., Micrococcus
the district of Dima Hasao has good health. The forest sp., Pseudomonas sp. The Bacterial identification was
in Ethenic village area of Halflong and Umranso are done with reference to Bergey’s Manual of
considered as forest having good health and rich old Determinative Bacteriology and that of fungus by Book
forest having ecological continuity. The fast pace of for Fungal Identification. The results and comparative
urbanization and other developmental activities within study revealed that both the ponds are highly polluted
the district will definitely have adverse effects on the with diverse microorganisms, some are harmful for
diversity and distribution of lichens in different forest health and the water not safe for the domestic use.
type and the present distribution pattern will act as base There has been great impact on physicochemical
line data for carrying out future bio-monitoring studies parameter like DO, BOD, COD that affects the every
in the area. component of ecosystems. The details and effect of
Key words: Biodiversity, Eastern Himalaya, Climate change, seasons are discussed in detail in paper.
Bio-monitoring. Key words: Microbiota, Biodiversity, Bacteria, Fungi, Water.

SII/P-27 SII/P-28

Comparative Study of Microbiota Diversity of Macrophytes and Microphytes

(Bacteria and Fungi) Diversity and in Budhatalab and Ranisagar of
Physico-Chemical Analysis of Rajnandgaon District, C.G.
Budhatalab and Ranisagar Ponds of
Anita Mahishwar1* and Shama Afroze Baig2
Rajnandgaon District, C.G. 1
Department of Botany, Govt. Digvijay Autonomous College,
Shama Afroze Baig * and Anita Mahishwar 2 Rajnandgaon, C.G., INDIA; 2Department of Microbiology,
Swami Shri Swaroopanand Saraswati Mahavidyalaya, Aamdi
Department of Microbiology, Swami Shri Swaroopanand Nagar, Hudco, Bhilai, C.G., INDIA, Email:anitamahiswar
Saraswati Mahavidyalaya, Aamdi Nagar, Hudco, Bhilai, C.G., @gmail.com, shamaabaig@gmail.com
INDIA; 2Department of Botany, Govt. Digvijay Autonomous
College, Rajnandgaon, C.G., INDIA, Email: shamaabaig@ The present study deals with the aquatic
gmail.com, anitamahiswar@gmail.com macrophytes and microphytes in Budhatalab and
Ranisagar of Rajnandgaon District, C.G. The goal of
The present study was aimed to compare the this study is to understand the importance of
microbiota (Bacteria & Fungi) in Budhatalab and microphytes and macrophytes in the preservation and
Ranisagar of Rajnandgaon District, Chhattisgarh. This restoration of the aquatic ecosystems and suggesting
study includes isolation and characterization of Bacteria the remedial measures in accumulation of toxic metals.
and Fungi present, their biodiversity in both the ponds, Macrophytes and microphytes are used as ecological
its correlation with physicochemical properties of water determinants and for phytoremediation. In order to avail
and seasons. In order to avail this we carried our study this we studied the diversity of two ponds in the vicinity
from July 2015 to June 2016. Aquatic fungi were of Digvijay College campus, Rajnandgaon.
isolated in different seasons by baiting techniques, and Macrophytes and Microphytes are well known
Bacterial estimation was done by SPC method. Pure biological filters and they carry out purification of the
culture was obtained and identified in lab. MPN test water bodies by accumulating dissolved metals and
was performed. Water samples were analyzed for toxins in their tissues. The study on diversity, abundance
various physico-chemical characters like temperature, and distribution of microphyte and macrophyte species
colour, turbidity, pH, Chloride, TDS, Dissolved oxygen, provides indicative information of environmental effect
BOD. Ranisagar showed a rich diversity in Fungi as on ecosystem. This work is the study survey undertaken

62 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

from August, 2015 to January 2017, which shows the from 36.3 to 201.8 Mgha”1 . Total C storage ranged
occurrence and distribution of the aquatic macrophytes between 16.3 and 92.8 Mgha”1. The range of Shannon–
in the selected ponds, and were classified according to Wiener diversity index was between 0.31 and
their habit. Both the ponds are filled with water all the 1.08.Furthermore most of the conifer-dominated forest
year round, its vegetation which includes 53 different types had higher C accumulation potential than
species of aquatic macrophytes were found from the broadleaf-dominated forest types. Protection of the
two ponds , species distribution and seasonal variation conifer-dominated forest stands from deforestation
are discussed in paper. 36 Algal genera were recorded especially Cedrus deodara, Abies pindrow, Pinus
from the class Chlorophyceae, Cyanophyceae and wallichiana and Pinus roxburghii would have the
Bacillariophyceae. Oscillatoria was found throughout largest per-unit-area-impact on reducing human-
the year. Chlorophycean microphytes dominated during induced C emission. The results also showed that the
the monsoon season and Cyanophycean microphytcs forest biomass is a multipart property that integrates
after monsoon. Bacillariophycean microphytes have no diverse ecological functional and structural attributes,
relation with the season. The high algal population could thereby linking growing stock volume density(GSVD),
be due to equal exposure of aquatic surface to sun and basal area, height, and wood density and hence plays a
pH. significant role in C accumulation, but not the tree
Key words: Macrophytes, Microphytes, Diversity, diversity. The results of the present study help in filling
Phytoremediation, Aquatic ecosystem. the knowledge gaps in scientific studies on the forest
ecosystems in this eco-fragile part of the Himalayan
SII/P-29 region and hopefully would help in mitigating the regional
climate change by minimizing the anthropogenic
Carbon Accumulation Pattern in impacts within these forest ecosystems in the region.
Different Forest Types of Northwestern Key words: C accumulation, Diversity, Forest ecosystems,
Northwestern Himalaya.
Himalaya, India: the Role of Tree
Structural and Species Diversity SII/P-30

Shiekh Marifatul Haq1*, Maroof Hamid2, Anzar A. Assessment of Plant Growth and
Khuro2 and Irfan Rashid1 Physiological Performance among
Department of Botany, University of Kashmir Srinagar- Eight Varieties of Chickpeas in Indo-
190006, J&K, INDIA; 2Centre for Biodiversity & Taxonomy,
Department of Botany, University of Kashmir, Srinagar- Gangetic Plains of Uttar Pradesh
190006, J&K, INDIA, Email: snaryan17@gmail.com
Shiv Naresh Singh1*, Shruti Mishra1, N. Manika1,
Forests are considered as the important sinks for
Bilal A. Khan1, Nayan Sahu1, Nalini Pandey2 and
atmospheric carbon (C), yet C uptake and accumulation
Soumit K. Behera1
rates are highly uncertain, and the mechanisms remain
poorly understood. The aim of the study was to assess Plant Ecology and Climate Change Science Division, CSIR
the tree structural attributes such as (basal area and - National Botanical Research Institute, Lucknow-226001,
height), diversity, stem density, biomass and C stocks INDIA; 2Department of Botany, Lucknow University,
Lucknow-226007, INDIA, Email: 777shivlko@gmail.com
in different forests of Northwestern Himalaya and to
make recommendations for forest management and Chickpeas are the most popular pulse crop of
conservation based on priorities for biodiversity India, popularly known as Gram or Bengal gram, mainly
protection and C sequestration. Eleven forest types in grown in Rabi season. Chickpeas are a rich source of
sub-tropical to temperate zones (400-2900 masl) of the highly digestible dietary protein. There is as such no
region were studied during the study. The results of detailed study available on growth, biomass and yield
the present study revealed that stem density ranged of different varieties in Chickpeas, Cicer arietinum
between 140 and 357 Nha”1, while total biomass ranged L. with special reference to Indo-Gangetic plains (IGP)

Abstracts 27-30 November, 2018, NBRI, Lucknow 63

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

of Uttar Pradesh. We compared the plant growth and loss caused by these species may soon surpass the
yield among 08 varieties of chickpea under farmer’s damage done by other anthropogenic activities.
field and screened the high yielding variety for Indo- According to IUCN, Alien Invasive Species are those
Gangetic region. Eight different varieties of Chickpeas alien species which become established in natural or
viz. HK94-134, JG -11, PANT-G -186, Radhey, semi-natural ecosystems or habitats, outside their
Avarodhi, Shubhra, Ujjwal and DCP-92-3 were natural distributional range and act as an agent of
analyzed in terms of growth, physiological attributes change causing threats to native biological diversity.
and yield in order to screen the best adaptive variety The opportunity of inadvertent introductions will may
with highest yield under rain fed conditions of IGP of become more with rapidly increasing global trade.
Uttar Pradesh. Field evaluation on the basis of During the course of floristic exploration of the Parvati
phenological traits, physiological performance, leaf area Aranga Wildlife Sanctuary and adjacent Tikri Forest
index and yield were carried out in randomized plots Area, Gonda district, Uttar Pradesh, the author has
with three replicates for each variety in Banda district recorded 381 species of flowering plants; out of these
of Uttar Pradesh. The measurements were made in 64 species belonging to 31 families analyzed as invasive
three growth stages i.e. vegetative phase, reproductive alien species. The species with American nativity
phase and senescence. We observed significant (Tropical America, Central America and South
differences in majority of evaluated traits among the America) were most dominant alien invasive, followed
08 varieties. Although, the morphological traits were by African, Asian, European and Mediterranean species
better in the variety ‘Ujjwal’ among all, but altogether, which hold the probability of their introduction through
‘Avarodhi’ observed the highest yield with maximum other agents along with the food grains, mainly by
photosynthesis rate. The above ground biomass was human beings. Further, the family Asteraceae
highest in Radhey variety with an average of dominated over all others with 09 taxa followed by
39.76±3.38 g plant-1 followed by Avarodhi (average Convolvulaceae, with 05 taxa, Euphorbiaceae and
30.33±2.64 g plant -1 ) and Shubhra. Highest total Papilionaceae with 04 taxa each. Invasive alien species
biomass was observed in Radhey (average 45.16±4.07 of the area and their impact on the wetland ecosystem
g plant-1) among all targeted varieties. From the present have been also discussed. A better planning is needed
findings, it may be concluded that Avarodhi has highest for early forecasting and reporting of infestation of
carbon sequestration potential with highest yield in the spread of these invasive weeds by creating plant
IGP of Uttar Pradesh in rain fed condition, which further detection network by collaborative links between
needs to be revalidated with more field trials at multiple taxonomist, ecologist and other stakeholders related to
locations. this field.
Key words: Chickpea, Indo-Gangetic plains, Uttar Pradesh. Key words: Alien species, Parvati Aranga Wildlife Sanctuary,
Tikri forest, Gonda.
Invasive Alien Flora of Parvati Aranga
Wildlife Sanctuary and Adjacent Tikri Aroma Yielding Grasses of India; A
Forest Area, Gonda District Uttar Potential Resource for Bioprospection
Pradesh, India Shailja Tripathi1,2*, Abhai K Srivastava1, Priyanka
Agnihotri1,2 and Tariq Hussain1,2
Vineet Singh 1
Plant Diversity, Systematics & Herbarium Division, CSIR-
Botanical Survey of India, Central regional Centre, National Botanical Research Institute, Rana Pratap Marg,
Allahabad-211002, Uttar Pradesh, INDIA, Email: Lucknow-226001, INDIA; 2Academy of Scientific and
vineet.singh332@gmail.com Innovative Research (AcSIR), Ghaziabad-201002, INDIA,
Email: tripathishailja16@gmail.com
Convention of Biological Diversity (1992)
visualizes that “biological invasion of alien species as Aromatic compounds have always been of great
the second worst threat after habitat destruction”. The significance due to a variety of chemical constituents

64 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

and have a diverse range of uses. Plant-based aroma an average of 0.40±0.17 alleles per loci. The neighbor
is of major choice for development of essential oil- joining (NJ) based clustering grouped all the accessions
based products whether it is pharmaceuticals, into three major clustering grouped were also confirmed
cosmeceuticals or perfumery industries. Moreover, by scatter plot of PCoA. Several potential accessions
nowadays aromatherapy has become the main line have been identified which could be of potential for
medication system for various health disorders. future T. cordifolia genetic improvement program.
However, despite the vast range of uses, extraction of Additionally, the grouping of various T. cordifolia
aromatic compounds has been limited to only a few accessions in three different clusters, as analyzed with
plant species resulting in the use of synthetic aromatic SSR markers, indicates that T. cordifolia has potential
compounds which in turn leads to the health side effects. inherent diversity that can be exploited towards genetic
Poaceae, the largest plant family in India comprising enhancement program.
1300 species belonging to 268 genera is of vital Key words: Tinospora cordifolia, microsatellite, genetic
significance useful in different economical aspects but diversity, NJ tree, PCoA.
its use is limited to only few species. In the present
paper, 14 grass species belonging to eight genera have SII/P-34
been documented for their aroma yielding properties.
Cymbopogon is found to be the most widely used grass Distribution and Current Status of
for aromatic properties, followed by Chrysopogon Thalictrum (Ranunculaceae) from
zizanioides, Eleusine indica, Eragrostis tremula, India
Thelopogon elegans, Sorghum arundinaceum,
Andropogon gayanus and Dicanthium sp. distributed Harsh Singh and Dibyendu Adhikari
in different parts of the country. The study is designed
with an idea of bioprospecting Indian grasses for their Department of Botany, North-Eastern Hill University,
aromatic and essential oil yielding properties on the basis Shillong-760002, Meghalaya, INDIA, Email: harshchamlegi@
of their traditional uses.
Key words: Aroma, Grasses, India, Bioprospection.
The genus Thalictrum L. (meadow rue) belongs
to the family Ranunculaceae and comprises 200 species
SII/P-33 mainly distributed in temperate regions of the world of
which twenty species with five varieties are found in
SSR Marker based Genetic Diversity India. The genus shows great complexities in its
morphological characters and is therefore a difficult
Analysis in Tinospora cordifolia and complicated genus for the identification. Throughout
the world, plants of this genus have been used as a
Suchita Lade 1,2 *, Hemant Kumar Yadav 1 and
general tonic, purgative, stimulant, aperient and for the
Veena Pande2
treatment of snake-bite, jaundice, rheumatism, wounds,
CSIR- National Botanical Research Institute, Lucknow- swellings, uterine tumours, paralysis, joints pain, nervous
226001, INDIA; 2Kumaun University, Nainital, INDIA, Email: disorders, toothache, diarrhea, piles, fever, peptic ulcers,
suchitalade.sl@gmail.com ophthalmic problems, headaches, as a tonic, diuretic,
The nature and extent of diversity in Tinospora etc. in all systems of medicine. Ecological niche
cordifolia, a dioecious climber, widely distributed in characterization and modeling potential distribution area
India, and one of the most important medicinal plants should help in developing strategies for its conservation.
has remained under explored. The present investigation In the present study, we used Maxent modeling to
aimed to explorethe level of genetic diversity in a larger characterize the environmental niche of the genus
set of germplasm of T. cordifolia using microsatellite Thalictrum and also assess the inter-specific niche
marker. A total of 96 accessions of T. cordifolia were differentiation. The finding of the study should be useful
evaluated for diversity analysis and SSRs markers in population survey and should be helpful in
developed for diversity assessment. A total of 77 alleles identification of sites for reintroduction and cultivation.
were amplified by 10 SSRs ranging from 4 to 12 with Key words: Thalictrum, Ranunculaceae, Maxent, Meadow rue.

Abstracts 27-30 November, 2018, NBRI, Lucknow 65

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SII/P-35 SII/P-36

Invasive Alien Plants of Balasore Studies on Invasive Plants in Central

District in Odisha India with Reference to Cassia
uniflora Mill.
Sandeep Kumar Nayak * and Kunja Bihari
Satapathy2 Vijay Kumar* and P.K. Khare
P.G. Department of Botany, Utkal University, Vani Vihar, Department of Botany, Dr. Hari Singh Gour University, Sagar-
Bhubaneswar, Odisha, INDIA; 2P.G. Department of Botany, 470003, M.P., INDIA, Email: vijaykumarju@gmail.com
School of Applied Sciences, Centurion University of
Technology and Management, Bhubaneswar, Odisha, INDIA Invasive species are those that occur outside their
Email: sandeepnayak75@gmail.com, kbsbotuu@gmail.com natural range, and cause negative effect on the
environment, ecosystems, habitats, native biodiversity
An exhaustive floristic survey was carried out
and even human health. Invasive species are also known
during August to December, 2015 in the Balasore district
by other several names such as non-natives, introduced,
to assess the diversity of invasive alien plants. From
exotic, non-indigenous and foreign species. Invasive
the study it was found that 93 species were invasive
alien species are plants, animals, pathogens and other
alien plants belonging to 71 genera and under 39
organisms that are non-native to an ecosystem, and
different angiospermic families. Analysis of habit
which may cause environmental harm or adversely
revealed that the herbs were dominant with 80 species impact biodiversity. All non-natives are not harmful.
(86.02%) followed by shrubs (9), climbers (03) and At least, 18% of the Indian flora constitutes adventives
trees (1). The dominant family was Asteraceae with aliens, of which 55% are American, 10% Asian, 20%
17(18.27%) species followed by Convolvulaceae (07), Asian and Malaysian and 15% European and Central
Amaranthaceae (04), Caesalpiniaceae (04), Fabaceae Asian species. Although a large numbers of exotics
(04), Poaceae (04), Solanaceae (04), Lamiaceae (03), are naturalized in India and have affected the distribution
Malvaceae (03) and Onagaraceae (03) etc. The of native flora to some extent, only a few have
important invasive species documented in the area conspicuously altered the vegetation patterns of the
under study were Chromolaena odorata, Lantana country. Present work deals with the occurrence and
camara, Hyptis suaveolens, Ageratum conyzoides, distribution of Cassia uniflora and its role in the extant
Parthenium hysterophorus, Eichhornia crassipes, communities. C. uniflora Mill. is a herbaceous invasive
Alternanthera philoxeroides, Mikania micrantha alien weed belongs to family Caesalpiniaceae. This plant
etc. The dominance of the members of Asteraceae is native of Tropical South America and widely
species among all IAPS found in the district was distributed in West Indies, Brazil and Mexico. In India,
resulted due to their higher potential for adaptability it was first reported from Eastern Karnataka in 1980
and rapid growth. It was found from the literature that by Raghavan, subsequently from Pune-Maharastra,
different native places of IAPS found from Balasore Andhra Pradesh, Kerala, Tamilnadu, Madhya Pradesh
district were Tropical America (54), Tropical Africa and Rajasthan. In Madhya Pradesh, it is widely
(11),Tropical South America (8), Mediterranean (3), distributed in Guna, Sagar, Dhar, Jhabua and other
West Indies (3), Tropical North America (3),Tropical places. C. uniflora is an aggressive weed occupying
Central South America (1), Tropical Central America large area of road sides, railway tracks and waste
(1), Tropical East Africa (1), Peru(1), Mascarene places. This species is spreading through road and
Islands (1), Temperate South America (1), Europe (1), railway transports, water drains and transport of
Brazil (1), and Tropical West Asia (1). The prevalence various agricultural products. Cassia uniflora has been
in large scale of IAPS in the district indicated the threat found as a dominant invasive species affecting the
to indigenous plant species and needs urgent measures native flora by altering the habitat. It competed through
to protect them. secreting phytoallelochemicals that affect the seed
germination, seedling growth and biomass production
Key words: Invasive alien plants, Balasore, Odisha
of other plants. Field observations indicate that

66 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Parthenium hysterophorus decline at places SII/P-38

dominated by Cassia uniflora and Cassia tora.
Therefore, C. uniflora and C. tora could be a potential Lectotypification of Boehmeria
species for the biological control of Parthenium densiflora Hook. & Arn. (Urticaceae)
and Hooker and Arnott’s Sets of
Key words: Invasive species, Ecosystem, Phytoallelo
chemicals, Cassia uniflora, Biodiversity, Parthenium Duplicates from Captain Beechey’s
hysterophorus. Expedition
Imtiyaz Ahmad Hurrah1*, Vijay Vishnu Wagh1 and
Do Invasive Alien Species Pose a Avinash Tiwari2
Threat to Rich Phyto-Diversity of Plant Diversity, Systematics and Herbarium Division, CSIR-
National Botanical Research Institute, Lucknow-226001,
Kinnaur District of Himachal Pradesh? Uttar Pradesh, INDIA; 2S.O.S. Botany Jiwaji University,
Gwalior-474011, Madhya Pradesh, INDIA, Email:
P. Sharma1, H.P. Singh1* and D.R. Batish2 saithimtiyaz18@gmail.com
Department of Environment Studies, Panjab University, The genus Boehmeria was named after Georg
Chandigarh, INDIA; 2Department of Botany, Panjab Rudolph Bohmer, a German botanist and physician.
University, Chandigarh, INDIA, Email: hpsingh_01@ Weddelt recognized a total of 47 species in 1869 relying
on the collections held at G, GDC, K and P. Willmort-
Kinnaur is a mountainous area and one of the Dear and Friis in 1996 recognized 33 species from old
most challenging terrains of India due to its geographical world and 14 from new world with no species occurring
location. It has not only great scenic beauty but is also in both old and new world. Boehmeria densiflora
rich in biodiversity. A preliminary survey was conducted distributed from south-western and southern China,
in Kinnaur district of Himachal Pradesh to study its Philippines and Japanese island group of Ryukyu and
flora and to find out the possible threat posed by Bonin with doubtful occurrence in Assam and Sikkim
invasive alien species, if any. During the survey, rich in India (confused with B. penduliflora). Hooker and
flora of the area including economically important plants Arnott (1838) while describing Boehmeria densiflora
was assessed. Some of the prominent species of the cited Loo Choo as the collecting locality for this species,
area encountered were Angelica sinensis, Plantago relating to Captain Beechey’s expedition form Ryukyu
ovata, Pinus gerardiana, Prunus armeniaca, Is, without providing information about collectors,
Cedrus deodara, Juniperus recurva and Betula utilis collection number or any other information which could
etc. Some of the plants such as Robinia pseudoacacia help to identify the herbarium material collected and
and Artemisia species were found exhibiting tendency where the material was deposited. Willmort-dear and
to spread in the area. Although, as of today, the area is Friis had cited as type of B. densiflora: Voyage of
relatively safer from the invasive alien species but a Capt. Beechey, Lay and Collie in herb Arnott s.n. (holo
few as mentioned above may pose threat to the local
E, n.v. [not see]). But two specimens with such criteria
species owing to the increasing temperature and
were found each in E and K. However the specimens
changing environment. Therefore, it is important to
that most closely associated with the work of Hooker
continuously monitor such species, assess their possible
and Arnott, seems to be the one deposited at K
risks and give early warning to the local people to
[K000741331]. Therefore the Herbarium sheet of B.
manage them before it is too late. The study bears
densiflora with bar code no. K000741331 is to be
importance since the invasive alien species are fast
chosen as the lectotype of this species.
spreading in the higher reaches of Himalayas and pose
a threat to the culturally and economically important Key words: Boehmeria densiflora, K000741331, Urticaceae.
species of the region.
Key words: Floral biodiversity, Invasive species, Risk
assessment, Early warning, Himalayas.

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SII/P-39 nitrogen congeners exhibit a relatively lower but

appreciable therapeutic efficiency to be used as
Hemi Synthesis, Computational and aromatase inhibitors.
Molecular Docking Studies of Novel Key words: Hemisynthesis, Nitrogen, CYP19, TESLAC
Nitrogen containing steroidal
Aromatase Inhibitors; Testolactum
and Testololactum Preliminary Studies on Algal Flora of
Suhelwa Wildlife Sanctuary in Uttar
Rayees Ahmad Lone*, Shabir Hassan Lone and
Vijay Vishnu Wagh
Pradesh with Special Reference to
Plant Diversity, systematic and Herbarium Division, CSIR-
National Botanical Research Institute Lucknow-226001, Utter
Pradesh, INDIA, Email: isaarhyder18@gmail.com Kiran Toppo* Sushma Verma and Sanjeeva
Testolactone and testolactone represent
Algology Laboratory, CSIR-National Botanical Research
aromatase inhibitors containing lactone rings.
Institute, Lucknow-226001, INDIA Email: toppokiran@yahoo.
Hemisynthesis of these compounds reported from the
most common phytosterols, whichare highly abundant
in nature. Herein, we report the synthesis of their Suhelwa wildlife sanctuary in Shravasti district
nitrogen congeners: testolactum and testololactam. The of Uttar Pradesh lies in proximity to India’s border with
reaction process involves the conversion of 4- lush forest of Mahadevpur in Nepal. The beautiful
androstene-3,17-dione to its corresponding oxime using sanctuary stretched over an area of approximately 452
hydroxylamine hydrochloride, whose Beckmann sq km is endowed with dense forest exhibiting rich flora
rearrangement under acid conditions yielded the desired with diverse habitats. Though the sanctuary
testolactum. However, testolactam was formed by the encompasses favorable ecological conditions for algal
Beckmann rearrangement of the oxime of 1-4- growth, the area remains unexplored until date.
androstene-3,17-dienome.This expeditious reaction Therefore, the present work is the first attempt to
scheme may be for the bulk production of testolalactum explore the algal flora of this sanctuary. Total number
and testolactum. Theoretical DFT studies concerning of 8 algal samples from different habitats were collected
the structural and electronic properties of all the end in the first phase of exploration. A detailed account of
products carrying out using the three parameter lee- morphotaxonomic description of all the identified taxa
yong Para function (B3LYP) and 6-31g (DP) level of was made. The identification resulted in altogether 25
theory. Molecular electrostatic potential map and taxa (11 genera) belonging to 4 classes. Within the
further orbital analysis were carried out. The HUMO- Chlorophyceae Desmids were dominant and
LUMO energy gap was calculated, which allowed the represented by Cosmarium pachydermum P. Lundell,
calculation of relative reactivity descriptors like C. phaseolus Brebisson ex Ralfs, C. dubium O.
chemical hardness, chemical inertness, chemical Borge, C. blyttii Wille, C. margaritatum (P. Lundell)
potential, nucleophilicity and electrophilicity index of Roy & Bisset, C. hammeri (Reinsch), C. dubium O.
the synthesized products. The molecular docking studies Borge, C. cyclicum P. Lundell, C. awadhense B.N.
testololactum, testolactum and testololactone with Prasad & R.K. Mehrotra, Closterium parvulum Nageli,
aromatase (CYP19) revealed binding free energies of Euastrum insulare (Wittrock) Roy. Class
(delta G b ) = -9885, -9.62 and -10.14 kcal mol -1 Cyanophyceae represented 5 taxa namely Geitlernema
respectively. Compared to the standard testolactone, a jasorvense (Vouk) Anagnostidis, Glaucospira
well-known aromatase inhibitor sold under the brand laxissima (G.S. West) Simic, Komarek & Dordevic,
name TESLAC, which exhibited a binding energy (delta Oscillatoria limosa Agardh ex Gomont, Nostoc
Gb of -10.29 kcal mol-1) with an inhibition constant Ki commune Vaucher and Anabaena doleolum
of 28.87 nm. The docking study revealed that the Bharadwaja. Similarly class Bacillariophyceae also

68 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

represented 5 taxa namely Spicaticribra grass (Doggrass/creeping cynodon), Nagarmotha,

kodaikanaliana Karthick & Kociolek, Hantzschia Shisam (Indian red wood), Dhatura(Thorn-apple),
amphioxys (Ehrenberg) Grunow, Pinnularia joculata Gulmohar (Gold mohur), Karanj, Baichandi (Wild Yam),
(Manguin) Krammer, Nitzschia palea (Kuetzing) Amla (Emblic-myrobalam/Amla), Safeda (Eucalyptus),
Smith and Stauroneis phoenicenteron (Nitzsch) Dudhi, Bargad, Bad (Banyan), Gular, Peepal (Pipal
Ehrenberg. Phacus pleuronects (O.F. Muller) Nitzsch tree), Anantmul ( Indian sarparilla), Chandrajot,
ex Dujardin, Phacus orbicularis K Hubner, Narangi (Lantana), Musakani, Mahua, Aam (Mango),
Trachelomonas hispida (Perty) F. Stein and Euglena Pudina (Mint), Chuimui(Sensitive plant), Gulbans (4’O
spirogyra Ehrenberg were represented by the class clock plant) Ban Karela (Bitter gourd), Munga (Horse
Euglenophyceae. The dominance of the desmid flora redish tree), Bach (Cowhage), Meethi Neem, Van kela,
indicates the specific type of habitats and may act as Kaner (Oleander), Tulsi (Basil), Bhuiaonla, Chitavar
indicators of pH change or the nutrient supply in the (Rove colour leadwort), Amrud (Guava), Sarpagandha,
above said sanctuary. Arandi (Castor), Shitaab, Semul (Silk Cotton tree),
Key words: Suhelwa Wildlife Sanctuary, Chlorophyceae, Patharchata (Indian rockfoil), Bhilwa, Cheeval,
Cyanophyceae, Biodiversity. Pithkarenti, Makoy (Black night shade), Bhatkataiyan,
Sahdehi, Chiraita(Chiretta), Jamun(Jambol), Imli
SII/P-41 (Tamrind), Sagon(Teak tree), Arjun (Arjuna), Baheda
(Beleric-myrabolam), Harra (Myrabolam), Giloy/
Patalkot: A Treasure of Medicinal Guduchi, Banda (Vanda/orchid), Sahadehi, Adrak
Plants and Biodiversity is under (Ginger), Ber (Plum), etc. Some very rare medicinal
plants like Siegesbeckia sp are also found in this valley.
Danger, Needs Conservation of Plants It is used in traditional medicine for syphilis, leprosy
and other skin illnesses. Some of the highly important
Neelima Ratti* medicinal plants, either are already extinct or are on
RRG Township, Bhojpur Road, Bhopal- 462047, M.P., INDIA, the verge of extinction from this valley. Over exploitation
Email: neelimaratti2010@gmail.com of plants like safed musli, chironji, sarpagandha and
Patalkot, a valley in Chhindwara district of bach has already made them endangered species.
Madhya Pradesh State, is known for its richness of During my survey of the valley, it was observed that
medicinal flora. This valley is covered with tropical the tribal (local) people of the area are using the plants
forests, which are rich in biodiversity. It is the home of of the valley for many diseases, as the medicine. Plants
about 200 rare medicinal plants and herbs. Few are also used as the wood, to make their homes, also
important and highly effective medicinal plants of this for making their food, by burning the wood etc. In this
valley includes: Addhajira (Chaff Tree), Bach(Sweet way, the treasure of herbal wealth of the valley is on
Flag), Adusa (Malabar nut), Bel (Bail fruit tree), the verge of extinction. People from other places are
Ajgandha, Lahsun (Garlic), Gwarpatha (Indian Aloe), also uprooting the herbal flora of the valley for their
Choulai (Amaranth), Soorankand (Corm), Kalmegh own selfish interests. Due to this extreme situation,
(Andrographis), Sarifa (Custard apple), Pili kateri the medicinal flora of the valley is in danger. Due to
(Mexican poppy), Narbod/ Satavar (Wild Asperagus), this scenario, it becomes very essential to make the
Kachnaar (Variegated mount in ebony), Punarnava local people aware of conservation of the plants used
(Spreading Hogweed), Shivlingi, Khatua (Sprout leaf extensively, by cultivating, propagating and multiplying
plant), Palas (Flame of Forest), Akona (Madar), Papita the plants, they are uprooting. The vast knowledge
(Papaya), Van Karonda, Ajwain (Bishop’s weed), Teeti possessed by the local people, about the plants, their
(Feted casia), Amaltaas (Pudding pipe tree), Brahmi uses & the herbal preparations they make, and dose of
(Indian pennywort), Safed musli, Hadjori, Neebu the medicine, should be documented as records for
(Lemon), Jangali Piyaz (Indian squill), Jaljamani, future generation.
Sankhpusphi, Dhania (Coriander), Kalimusli, Kalihaldi, Key words: Patalkot, Conservation, Medicinal Plants,
Amarbel (Dodder), Gautichai (Lemon grass), Doob Extinction, Biodiversity

Abstracts 27-30 November, 2018, NBRI, Lucknow 69

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

70 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Session III
Environmental Biotechnology
and Microbiology

Abstracts 27-30 November, 2018, NBRI, Lucknow 71

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

72 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)


Regulation of Submergence Tolerance Application of Beneficial Rhizospheric

in Rice by a Mitogen Activated Protein Bacteria for Increasing Crop
Kinase Productivity of Saline Soils
Alok Krishna Sinha* Naveen Kumar Arora
National Institute of Plant Genome Research, Aruna Asaf Department of Environmental Science, School for
Ali Road, New Delhi 110067, India, Email: alok@nipgr.ac.in Environmental Sciences, BBA University, Lucknow-226025,
INDIA, Email: nkarora_net@rediffmail.com
Mitogen activated protein kinase (MAPK/MPK)
cascade are known to regulate several stress responses Soil salinity is a major abiotic stress that reduces
and developmental processes in eukaryotes. They crop yields by affecting approximately 20% of irrigated
usually consist of three tier components comprising of land at global level. Soil salinity also causes the
a MAPK, MAPK kinase (MAPKK/ MAP2K/ MKK) reduction of soil quality along with crop production. To
and MAPKK kinase (MAPKKK/ MAP3K/ MKKK) overcome this problem application of salt tolerant plant
connected to each other with an event of growth promoting rhizobacteria (PGPR) as bio-
phosphorylation. In the present talk, the role of this inoculants can be a very beneficial technique. Salt
important cascade will be discussed in light signal tolerant beneficial rhizospheric bacteria mainly
transduction pathways during seedling development in fluorescent pseudomonads and rhizobia were isolated
Arabidopsis and submergence tolerance in rice. The and identified from diverse host plants growing in saline
activation and interaction of AtMPK6 with MYC2 regions. Some potent salt tolerant PGPR (exhibiting
transcription factor in a light dependent manner will be various PGP attributes such as phosphate and zinc
discussed. AtMKK3 was identified as upstream kinase solubilisation, production of siderophore, indole acetic
of AtMPK6. The role of MYC2 has been deciphered acid and exopolysaccharides) were selected for field
as a negative regulator of blue light signaling in applications. Selected bacterial strains were applied as
Arabidopsis. In rice we identified regulation of bio-inoculants on various crops such as sunflower,
SUB1A1, a transcription factor identified as an chickpea and lettuce in saline soils of farmer’s fields.
important molecule in conferring submergence On application of bio-inoculants productivity and quality
tolerance in rice to be regulated by a MAP kinase, of crops (sunflower, chickpea and lettuce) was
OsMPK3. SUB1A1 was specifically identified as a significantly improved in comparison to control.
interacting partner as well as phosphorylation target of Beneficial rhizospheric bacteria based bio-inoculants
OsMPK3 in a submergence dependent manner. In both were also very effective to enhance the crop
the above mentioned signalling pathways we have productivity even in presence of phytopathogens
conducted series of physiological, biochemical, (Macrophomina phaseolina) in field conditions.
molecular and genetics related experiments to These strains/bio-inoculants can be used for enhancing
empahsize the pleiotropic roles of MAP kinase cascade crop productivity in saline soil along with combating
in plants. phytopathogens in beneficial way for safe and
sustainable agriculture. This will also help in remediation
Key words: Rice, Mitogen activate protein kinase,
Arabidopsis, light signaling. of saline soils.
Key words: Salinity, PGPR, Bio-inoculant, Fluorescent
pseudomonads, Rhizobia.i

Abstracts 27-30 November, 2018, NBRI, Lucknow 73

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SIII/L-2 arsenic accumulation not only in grain but also in straw,

feed for livestock.
Biotechnological Approaches for Key words: Arsenic, Growth, Rice, Ceratophyllum demersum,
Reducing Grain Arsenic Accumulation Westerdykella aurantiaca.
in Rice SIII/L-3
Debasis Chakrabarty Heavy Metals Phytoextraction
Biotechnology & Molecular Biology, CSIR-National
Botanical Research Institute, Lucknow-226001, INDIA,
Potential of Native Plants and their
Email: debasis1972@rediffmail.com Histological Observation Growing on
Rice (Oryza sativa L.)  is  amidst  the  great Stabilized Distillery Sludge: A
essential food crop that offers a staple food for most Prospective Tool for in situ
of the world’s population. Arsenic (As) is a
carcinogenic heavy metal, which harms human health. Phytoremediation of Hazardous
In Asian countries, a major source of human As-intake Industrial Waste
is rice grains and; contamination of paddy soils and As
accumulation in rice grains is one of the serious Ram Chandra
agricultural issues. Hence, it is important to mitigate Department of Microbiology, School for Environmental
the effects of As toxicity as much as possible. The Sciences, Babasaheb Bhimrao Ambedkar University (A
main objective of our research is to provide an overview Central University), Lucknow-226025, Uttar Pradesh, INDIA
of various biotechnological methods exploited Email: rc_microitrc@yahoo.co.in
for reducing As accumulation in rice grain. To restrict
As in the rice roots as a detoxification mechanism, a The sugarcane molasses based distillery waste
transgenic approach has been followedthrough contains significant amount of different heavy metals
theexpression of phytochelatin synthasefrom along with organometallic Compounds. The maillard
Ceratophyllum demersum (CdPCS1), an aquatic products present in distillery waste strongly bind with
plant. Transgenic lines showed enhanced accumulation heavy metals and other cationic molecules. This makes
of As in root and shoot but less in grains. We also more complex and hazardous nature of waste. Due to
describe a glutaredoxins (Grx) family protein designated complexity of sludge it causes the toxicity to environment
as OsGrx_C7, and investigate the mechanism of at different and makes irreparable damage to
glutaredoxin mediated arsenic tolerance and ecosystem. Therefore, the study has been focused to
accumulation in rice grains. Overexpression of analysis metallic and organic contents of sludge. This
OsGrx_C7 conferred a markedly enhanced tolerance study revealed that distillery sludge contains not only
to arsenite and reduces arsenite accumulation in seeds mixture of complex organic pollutants but also retains
and shoots of rice. Recently, we showed that OsPRX38 high quantity of Fe (5264.49), Zn (43.47), Cu (847.46),
transgenics accumulate less As due to high lignification Mn (238.47), Ni (15.60), and Pb (31.22 mg kg-1) which
in root which acts as a barrier for As entry in transgenic enhances the toxicity of sludge to the environment. The
plants. Another potential strategy is to generation of major identified organic compounds were benzene, 1-
genetically engineer plants with arsenic ethyl-2-methyl, benzene, 1-ethyl-4-methyl benzoic acid,
methyltransferase gene capable of methylating and 3,4,5-tris(TMS oxy), TMS ester; hexanedioic acid,
volatilizing arsenic. Arsenic methyltransferase dioctyl ester; stigmasterol TMS ether; 5á-cholestane,4-
(WaarsM) gene from Westerdykella aurantiaca was methylene; campesterol TMS; â-sitosterol and
cloned and demonstrated that the resulting transgenic lanosterol. These compounds are listed under the EDCs
plant acquired the potential for methylating inorganic also as per U.S. Environmental Protection Agency.
arsenic to a variety of innocuous organic species, However, the phytoextraction potential of growing
including volatile arsenicals, providing a potential native weeds and grasses i.e. Argemone mexicana,
strategy for potent transgenic rice capable of low Saccharum munja, Cynodon dactylon, Pennisetum

74 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

purpureum, Chenopodium album, Rumex dentatus, constituent which consists of polysaccharides, lipids,
Tinospora cordifolia, Calotropis procera and protein and DNA especially uronic acids, pyruvate, and
Basella alba revealed the high accumulation of Fe, O-methyl, Oacetyl, and sulfate groups. Cyanobacteria
Zn, Cu, Mn, Ni, and Pb in their root and leaves is one of the most efficient microbial community platform
compared to shoot. This indicated high accumulation to naturally synthesize diverse range of therapeutics
and translocation capabilities of these plants. Further, biomolecules. In the present study, six fast growing
the bioaccumulation coefficient factor (BCF) and nitrogen fixing filamentous cyanobacteria representing
translocation factor (TF) was found >1 for majority of the genus of Nostoc, Anabaena and Plectonema
plants for various metals. Thus, this given strong having EPS production in the range of 1.01-1.58 µg ml-
evidence for hyperaccumulation tendency of these were identified, biochemically characterized and
native weeds and grasses from complex polluted sites. observed their flocculating activities. Highest levels of
Furthermore, the ultrastructural observations of root EPS were produced by Nostoc sp. when nitrogenase
tissues also revealed the deposition of heavy metals at activity was very low. Maximum EPS production
various cellular components without any apparent toxic occurred at pH 8.0 in the absence of any combined
effects. This indicated the variable adaptive nitrogen source. The cyanobacterial EPS consisted of
characteristics of these plants growing at a hazardous soluble proteins and polysaccharides that included
waste polluted site. Thus, the study given a strong substantial amounts of neutral sugars and uronic acid.
evidence for application of these weeds and grasses Another set of twelve nitrogen fixing cyanobacteria
as tools for in-situ phytoremediation and eco- represent the genus of Nostoc and Anabaena having
restoration of polluted sites contaminated site and EPS production in the range of 0.80-1.66 µg ml-1 were
distillery sludge after phytoremediation showed a characterized. The EPS isolated from Anabaena sp.
sustainable development of ecosystem. and Nostoc sp. demonstrated high flocculation capacity.
Keywords: Native weeds; Endocrine-disrupting chemicals; There was a positive correlation between uronic acid
Distillery sludge; In-situ phytoremediation, â-Sitosterol content and flocculation activity. These strains were
executed for production of EPS, PBS, Chl a and
SIII/O-1 bioflocculant activity. Based on this current global
scenario, the main purpose of this study is to identify
Cultural Studies and Biochemical diverse cyanobacterial regime for its
Characterization in High Yielding biophysicochemical characterization and screening high
yielding EPS and carotenoid producing cyanobacterial
Carotenoids and Exopolysaccharides communities towards commercial exploitation of
Producing Cyanobacteria suitable candidate.
Key words: Carotenoids, Exopolysaccharide, Cyanobacteria,
O.N. Tiwari Ammonia Excretion, Phycobiliproteins.
Centre for Conservation and Utilisation of Blue Green Algae,
Division of Microbiology, ICAR-Indian Agricultural Research SIII/O-2
Institute, New Delhi, 110012, INDIA, Email:
ontiwari1968@gmail.com Beneficial Microbial Services in
Blue green algae or cyanobacteria have been
Management of Agro-Environmental
emerged as a sustainable bioresource of novel bioactive Problems
secondary metabolites. Bioactive secondary
metabolites include value added pigments, food Jay Shankar Singh
colorants, cosmetics, feed additives, polyunsaturated Department of Environmental Microbiology, Babasaheb
fatty acids, exopolysaccharides, vitamins, lipids, Bhimrao University, Lucknow-226025, Uttar Pradesh, INDIA
proteins and therapeutic agents i.e. antioxidants, anti- Email address: jayshankar_1@yahoo.co.in
inflammatory etc. EPSs of cyanobacteria are anionic Land use/cover changes, amongst the most
high molecular weight heteropolymeric extracellular influential environmental issues, are negatively

Abstracts 27-30 November, 2018, NBRI, Lucknow 75

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

impacting the soil quality and sustainability. Currently Key words: Agro-environmental sustainability, Ecosystem
it is uncertain how the soil microbial communities and services, Microbial agents, Methanotrophs.
biomass, the foundation of soil fertility support system
will be influenced by anthropogenic mediated land use SIII/O-3
disturbances. It is also not clear how land cover changes
may influence the soil microbial biomass levels and
Isolation, Growth and Identification of
fertility relevance across different soil depths in a given Chloropyrifos Degrading Bacteria
forest or agro-ecosystem. The changes in soil microbial from Agricultural Soil
biomass levels due land use changes may also adversely
affect the soil physico-chemical properties, fertility and Sonal Suman, Satyamvadaswayamprabha and
ecosystem stability. The increasing disturbances in Tanuja Singh*
agriculture and environmental resources due to
urbanization and over exploitation of natural resources Department of Botany and Biotechnology, TPS College,
Kankarbag, Patna-80001, INDIA, Email: sonal.micro89@
create a great concern to the peoples of all over the
gmail.com, tanujasinghbotany@gmail.com
world. The anthropogenic activities led to climate
changes such as drought, flood, and irregular rainfall, Pesticides are a large and varied group of
forest fires, habitat loss, water scarcity, poor agricultural substances that are specifically designed to kill
productivity, soil and environment degradation, etc. organisms including of weeds, insects, and the
These disturbances ultimately impact the sustainability indiscriminate use of pesticides in agricultural field
of agriculture, environment, economy and health. resulted into contamination of soil environment leading
Although global climate change due to increase in green to toxicity. India is an agriculture based country and
house gases such as atmospheric methane (CH 4), maximum portion of it’s economy is dependent on
affect all over the world, it becomes more vulnerable agriculture. The promotion of high yielding varieties of
to developing countries which mainly depend upon crops has led to large scale use of chemicals as
agriculture and agriculture based economy. pesticides. Chlorpyrifos, is one of the most commonly
Management of agricultural yields is a major concern and widely used commercial orgnophosphate pesticide.
for agricultural sustainability, however, until now It is a broad-spectrum, moderately toxic pesticide that
synthetic chemicals have played a fundamental role in has been widely used in the prevention of both
suppressing pests and maintaining high crop yields but agricultural pests and urban public health pests. It was
also responsible for soil degradation and pollution. Soil introduced in 1965 by Dow Chemical Company India.
pollution not only affects the area where it applied but It has large blights on public health and environment
also creates pollution to nearby water bodies, streams resulting from its long residual period in soil and water.
and rivers. They also disrupt the natural ecosystem The microbial action in the environment causes the
and soil microbial diversity, which plays a vital role to natural degradation of the pesticides which might
keep up soil sustainability and health. Bio-agents are convert parent compounds to intermediates or
primarily living microorganisms like fungi, bacteria and comparatively less toxic compounds. The adaptability
cyanobacteria which is used for the controlling of many of microorganisms during bioremediation releases
ecosystem services. These bio-agents provide good certain enzymes, which metabolizes wide spectrum of
agricultural productivity and also enhance soil fertility anthropogenic chemicals. The current method for
through improving natural agro-ecosystem. Some of removing such contaminants from the environment
the bio-agents like cyanobacteria, mycorrhizae, through biodegradation has been shown to be more
methanotrophs, etc., are widely used in restoration of effective than any other method. In the present study,
degraded lands and green house gas -CH4 mitigation. soil sample was collected having history of Chlorpyrifos
Microbial agents are not in optimum use in most from four different soil sample (rice, wheat, maize and
developing countries due to technical, social and vegetable). Nine Chlorpyrifos pesticide utilizing bacteria
institutional constraints. The effectiveness and efficacy were isolated and identified through cultural and
of microbial agents and their resources have yet to be biochemical tests as strains of Bacillus sp.,
explored to become alternate of agro-chemicals. Staphylococcus sp. and Coccus sps. Their growth in

76 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

minimal salt medium supplemented with 200 and 250 0.5 mM Cd + PSB. Spinach grown with Cadmium
µg ml-1 of Chlorpyrifos was monitored at optical density showed a decrease in biomass, chlorophyll content and
600 nm. The result showed that Staphylococcus sp., increase in MDA indicating a dysfunction in
Streptococcus sp. and Bacillus sp. had maximum photosynthetic apparatus. Nutrient analysis in spinach
growth at twelve days, while Coccus sp. Gram-ive showed a decrease in sodium (59%), potassium (24%),
Bacillus sp. shows highest growth upto four days of calcium (42%), iron (21%), magnesium (67%),
incubation at 200 µg ml-1 and upto eight days of manganese (32%), copper (22%), nitrogen (18%) and
incubation at 250 µg ml-1 of Chlorpyrifos. The results phosphorus (16%). With higher exposition time, the
of this research indicated that the isolated bacteria can plant was observed to have accumulated 20 mg kg-1 in
be used for bioremediation of Chlorpyrifos shoots and 5 mg kg-1 in roots. The plant showed a strong
contaminated soil. defense mechanism against the oxidative stress with
Key words: Isolation, Bacteria, Chlorpyrifos, Contaminated, the activation of CAT, GPOX and GR indicating the
Agricultural soil. active involvement of glutathione in the plant tolerance
mechanism. While in the plants treated with Cadmium
SIII/O-4 + PSB, there was a decrease in metal accumulation
by 20-25% and increase in phosphorus content by 30%
Potential of Phosphorous Solubilizing as compared to the plants treated with only Cadmium.
Bacteria (PSB) in Improving the Our study indicates that PSBs can influence the
bioavailability of heavy metals in soil, thereby decreasing
Palatability of Vegetable Crops the bioaccumulation in plants.
Grown in Heavy Metal Contaminated Key words: Antioxidative enzyme, Cadmium, Oxidative stress,
Environments PSB, Spinach.

Sushmita Barua1*, Dibyendu Adhikari1 , Saroj K. SIII/O-5

Barik1,2 and Nikhil K. Chrungoo1
Production of Novel Antibacterial
Department of Botany, North-Eastern Hill University, INDIA;
Present address: National Botanical Research Institute, Polyhydroxyalkanoates (PHAs)
Lucknow, INDIA, Email: nchrungoo@gmail.com Incorporating Medicinal Plant Extract
Heavy metal contamination of terrestrial and
aquatic systems is a serious environmental issue. Tanushree Halder1 , Sheila Azim Piarali 2 , Pooja
Irrigation of agricultural land with contaminated water Basnett2 and Ipsita Roy2
often leads to heavy metal accumulation in the 1
Department of Genetics and Plant Breeding, Sher-e-Bangla
vegetables and crops growing on such sites. Vegetable Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207,
crops such as spinach can accumulate heavy metal BANGLADESH; 2Department of Life Science, University of
without any visual indication of stress. Consumption of Westminster, 101 New Cavendish Street, London W1W6XH,
such contaminated vegetables might lead to serious UK, Email: tanushree.halder01@gmail.com, S.Azimpiarali@
westminster.ac.uk, p.basnett@my.westminster. ac.uk, I.Roy01@
health issues in human beings. Therefore, it is essential
to find a solution to this problem. Phosphorus solubilizing
bacteria (PSB) has been reported to influence the Polyhydroxyalkanoates (PHAs), a group of
uptake of such contaminants. We tested this hypothesis polymers have become potential candidates in
using spinach as a model species and cadmium as a biomedical, agriculture and industry due to its
contaminant. Cadmium (Cd) - a highly toxic heavy biocompatibility, biodegradability and wide range of
metal which attracts attention because of its widespread mechanical properties. However, multidrug resistant
occurrence and its carcinogenic property. We studied (MDR) bacterial infections spread through polymer
the oxidative stress and biochemical responses of fabricated devices and tools used in various sectors as
spinach grown in three concentrations of cadmium i.e., bacteria adhere and breed on its surface resulting high
0.1 mM, 0.3 mM, and 0.5 mM, and in combination of morbidity, mortality and environmental pollution. To

Abstracts 27-30 November, 2018, NBRI, Lucknow 77

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

prevent this ongoing threat, it is essential to develop SIII/O-6

polymer having inherent antibacterial properties. Novel
antibacterial Poly(3-hydroxybutyrate), P(3HB): a short Therapeutic Role of Aqueous Leaf
chain length PHA was produced by direct incorporation Extract of Phyllanthus niruri on
of Eucalyptus globulus oil (EO). P(3HB) was
produced by batch fermentation of Bacillus subtilis Plasticizer Induced Renal Toxicity in
OK2. Identification of the polymer was done by fourier Mice Model
transform infrared spectroscopy (FTIR), gas
chromatography-mass spectrometry (GC-MS) and Anjali Singh1*, Tanuja Singh1, Ravish Kumar2 and
nuclear magnetic resonance (NMR). Agar disc diffusion Jitendra Kumar Singh2
assay, minimum inhibitory concentration (MIC) and 1
Department of Botany, Thakur Prasad Smriti College,
minimum bactericidal concentration (MBC) were Patliputra University, Patna, Bihar, INDIA; 2S.S. Hospital and
performed as antibacterial assays against Research Institute, Patna, Bihar, INDIA, Email:
Staphylococcus aureus ATCC 6538 and Escherichia dranjalisingh04@gmail.com, tanujasinghbotany@
coli ATCC 8739. Oil incorporated P(3HB) exhibited gmail.com, kumardrravish@gmail.com
2.4 cm and 1.1 cm halo against S. aureus and E. coli, The major part of the population in both developed
respectively. Liquid FTIR confirmed the presence of and developing countries as well as in rural and urban
oil into the polymer. Thermal and mechanical analysis areas are getting exposed to plasticizer which are
of neat and oil incorporated P(3HB) were done by reported to dissolve (by leaching) with the contents
differential scanning c calorimetry (DSC) and tensile (medicines, edibles etc.) present therein. Plasticizer
testing, respectively. Oil incorporated P(3HB) exhibited exposure is a major health problem due to its toxic effect
5.6% decreased crystallinity and reduced thermal in animal model as well as human. Oral administration
temperature. The elongation at break increased from of plasticizer at environmentally significant dose for a
6.9±1.7 to 27.9±3.5% while Young’s modulus and period of six weeks showed signs of renal toxicity in
tensile strength decreased drastically. Therefore, mice model. Significant alterations in biochemical
developed antibacterial polymer exhibited elastomeric markers of renal function as well as in cyto-architectural
properties. Scanning electron microscopy (SEM) and structure of nephrocytes was observed. The alterations
contact angle exhibited hydrophobic and rougher were found to be statistically significant (P<0.01).
surface of the novel polymer. However, non-significant Phyllanthus niruri is a common herb that is widely
protein adsorption, higher cytotoxicity against C2C12 used for its therapeutic effects. Curative properties of
cell line and lower biodegradability were exhibited and this medicinal plant are due to the presence of bioactive
thereby suggesting optimisation of oil concentration into phytochemicals, among which polyphenols are reported
the polymer. Indeed, EO incorporated P(3HB) film to be most potent P. niruri is popularly known as stone
exhibited diverse application options not only because breaker. The plasticizer treated mice were
of its antibacterial properties but also for excellent therapeutically administered aqueous leaf extract for
thermo-mechanical properties. four weeks period. Therapeutic treatment of P. nuriri
Key words: Poly(3-hydroxybutyrate) [P(3HB)], Bacillus on plasticizer exposed mice revealed marked
subtilis OK2, Eucalyptus globulus, Multidrug resistant improvement in cyto- architectural structure of
bacteria, Minimum Inhibitory Concentration. nephrocytes and in renal biochemical markers.
Key words: Phyllanthus, Renal, Toxicity, Antioxidant,
Therapeutic, Biochemical markers.

78 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)


Pharmaceutical Product in Identification of Endophytic Fungal

Environment-Sources, Potential Biodiversity Isolated from Critically
Human Health Impacts, Emergence of Endangered Endemic Medicinal Plant,
Antibiotic Resistance Gene (ARGs) Artemisia amygdalina Decne. of
and Current Remediation Kashmir Himalayas
Humeera Nisa*1and Azra N. Kamili2
Hemen Sarma 1
Department of Environmental Sciences/Centre of Research
for Development, University of Kashmir, Srinagar-190006,
Department of Botany, N.N.S. College (Dibrugarh
J&K, INDIA; 2Department of Environmental Sciences/Centre
University), Titabar-785630, Assam, INDIA, Email:
of Research for Development, University of Kashmir,
Srinagar-190006, J&K, INDIA, Email: humeranissa2@
The multiple uses of pharmaceutical products in gmail.com, azrakamili@gmail.com
healthcare, livestock’s and agriculture, leading to their The literal meaning of the word endophyte means
infiltration into the environment have received a great ‘in the plant’ (endon Gr., within; phyton, plant). The
attention. In recent years adverse effects of usage of this term is as broad as its literal meaning and
pharmaceutical products specifically antibiotics on has a broad spectrum of potential plant hosts and
animal and human health has been reported worldwide; inhabitants, including bacteria, fungi, algae, and insects.
this is because of the development of antibiotic-resistant An endophytic fungus can be defined as an organism
and multi-drug resistant bacterial strains. Furthermore, which lives in mycelial form in biological association
these emerging pollutants also have lethal effect for with living plant at least for some time. The minimum
normal microbiota beneficial for environment. requirement before a fungus to be termed as an
Antibiotics are most extensively administered drug as endophyte should be the demonstration of its hyphae
therapeutic for precaution, safeguard and treat humans in the living tissue. Fungi are key resources for
and animal diseases. There is a worst-case scenario exploiting bioactive metabolites. Among fungi,
reported in developing worlds; it was estimate, 700,000 endophytes are important to screen biologically active
people currently succumb to antibiotic-resistant metabolites. The genus Artemisia L. (Asteraceae)
infections every year. The antibiotics residues present containing 500 species is the largest genus in the tribe
in the environment contribute to the development and Anthemideae. Several Artemisia species have
dissemination of antibiotic resistance genes (ARGs) in medicinal importance and are used in traditional
bacteria through selection. Most of these contaminants medicine. This endemic and critically endangered
remain persistent in soil aqueous phase and in waste medicinal plant grows in the subalpine region of Kashmir
water. There is growing evidence that antibiotics along Himalayas and is also found in the North-West Frontier
with other pharmaceutical products confer selection Province of Pakistan. The plants are locally used for
pressure in favour of antibiotic resistant bacteria. The the treatment of a number of diseases like epilepsy,
removal or degradation of these contaminants from piles, nervous disorders, cough, cold, fever and pain.
environment is one of the biggest challenges. This important medicinal plant of Kashmir valley was
Key words: Pharmaceutical products, Emerging Pollutants, chosen for this study because of its pharmacological
Antibiotic Resistance Genes, Solutions. and therapeutic importance in the folkloric medicines.
Fresh plant material was used for isolation work using
surface-sterilization technique. The fungi have been
identified on the basis of their morphological and cultural
characteristics using standard taxonomic keys and
monographs. A total of twenty-seven (27) fungal

Abstracts 27-30 November, 2018, NBRI, Lucknow 79

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

endophytes were isolated from this medicinal plant also significantly increased shoot biomass without
belonged to twenty-four (24) different species. elevating As accumulation in PvPht1;2 transgenic
Key words: Fungi, Endophytes, Endemic, Artemisia tobacco. Taken together, our results demonstrated that
amygdalina, Kashmir Himalayas. PvPht1;2 is a specific P transporter responsible for P
acquisition and translocation in plants. On the other
SIII/O-9 hand, enhancing PvPht1;3 increased arsenate uptake
and translocation in tobacco plants. As concentrations
Identification and Functional Analysis in shoots of PvPht1;3-Oxwere 29-38%higher after
of Phosphate Transporters in AsV exposure for 1day than that of WT, respectively.
AsV uptake rate, As concentration in xylem sap and
Phosphate and Arsenate Absorption translocation factor were increased compared to wild
and Transport in Pteris vittata type plants with different degree, respectively. It’s
suggested that PvPht1;3 played key role in efficient
Yue Cao*, Dan Sun, Yanshan Chen and Lena Q. absorption and transport of arsenic in Pteris vittata.
Ma In this study, diverse functions of Pteris vittata PHT
transporter in arsenate and phosphate uptake pathway
State Key Lab of Pollution Control and Resource Reuse,
School of the Environment, Nanjing University, Nanjing, were preliminarily identified. We expect to use the key
Jiangsu 210023, CHINA, Email: caoyue@nju.edu.cn gene, PvPHTs, to improve the efficiency of
phytoremediation in future.
Pteris vittata, the first arsenic hyperaccumulator
identified, has very strong ability to absorb the arsenic, Key words: Pteris vittata, Nicotiana tabacum,
Phytoremediation, Transcriptome, Hyperaccumulator.
so it is widely used in phytoremediation of arsenic
contaminated soil. However, the molecular mechanism
of arsenic uptake efficiency of Pteris vittata is not
clear. As a phosphorous analog, arsenate is absorbed Sustainable Solutions for Arsenic
mainly by the phosphate transporter Pht1. Using the
transcriptome sequencing and gene cloning, five Stress Mitigation and Arsenic
members of Pteris vittata Pht1, have been identified. Reduction in Rice
We determined their arsenic transport capacity through
the analysis of arsenic content in different Pht1 strains Sudhakar Srivastava
of yeast transformation lines. By qRT-PCR, expression Plant Stress Biology Laboratory, Institute of Environment
pattern of PvPht1 protein have been identified, and Sustainable Development, Banaras Hindu University,
PvPht1;2 and PvPht1;5 transcripts were induced to Pi Varanasi-221005, INDIA, Email: sudhakar.srivastava@gmail.com
starvation and PvPht1;4 expression level was increased
in response to As exposure. PvPht1;2-PvPht1;5 were The problem of geogenic arsenic contamination
all localized to plasma membrane, by expression in is widespread in Gangetic plains in Indian states of
tobacco leaves with GFP fusion protein, respectively. Uttar Pradesh, Bihar and West Bengal. In this region,
Further, PvPht1 transporters were transformed into arsenic concentration of groundwater is several folds
tobacco (Nicotiana tabacum), respectively. In higher over the safe limit and toxic levels of arsenic
hydroponic experiments, all PvPht1;2-Ox lines have been reported in crop plants, vegetables, fruits
displayed markedly higher P content and better growth and fish and meat products. In the affected states, rice
than wild type plants, suggesting that PvPht1;2 mediated is the main crop and staple food for humans. Rice is
P uptake in plants. In addition, expressing PvPht1;2 more efficient in arsenic accumulation in its grains than
also increased the shoot/root 32P ratio by 69-92% and other cereal crops. The problem can be tackled either
enhanced xylem sap P by 46-62%, indicating that through remediation of contaminated water or through
PvPht1;2 also mediated P translocation in plants. Unlike management of agronomic practices for growing safe
many Phts permeable to AsV, PvPht1;2 showed little rice. Further, there are known variations in rice
ability to transport AsV. In soil experiments, PvPht1;2 genotypes in terms of arsenic accumulation in their

80 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

grains. The grains of these contrasting genotypes show their relationship and the underlying mechanisms have
variations in accumulation of other mineral elements not been investigated. From this backdrop, pot
as well as in amino acid contents. Hence, genotype experiments were carried out to determine interplay
screening and selection and development of suitable between Zn (500 and 1000 mg kg-1 dry soil) and AM
genotype for cultivation in affected regions are fungus (Funneliformis mosseae) on growth, nutrient
promising approaches. With respect to agronomic acquisition, oxidative stress and non enzymatic
practices, supplementation of rice crops with redox antioxidants in two pigeonpea [Cajanus cajan (L.)
active chemical, thiourea, has been proved to be a Millsp.] genotypes (Tolerant- Sel 85N and Sensitive-
promising approach in lab conditions. In addition, other P792) exposed to Cd stress (25 and 50 mg kg-1 dry
chemical (nitrogen) and biological (microbial consortia) soil). Results revealed that accumulation of Cd and Zn
amendments could pave way for reducing arsenic in reduced plant biomass, disrupted ionic balance by
rice grains. Hence there lies possibility of its application interfering with the uptake and translocation of various
in field conditions for safe rice cultivation. The nutrients (N, P, K, Mg, Ca, Fe), induced membrane
presentation highlights key findings in the direction of destabilization, hydrogen peroxide (H2O2) accumulation
suggested possible approaches to tackle the issue of and increased non-protein thiols (NP-SH) [glutathione
arsenic contamination of rice. (GSH), glutathione disulphide (GSSG) and
Key words: Arsenic, Genotype, Nitrogen, Microbial consortia, phytochelatins (PCs)], with Cd being more toxic in
Rice. P792 than Sel 85N. Zn supplementation reduced the
uptake of Cd which further declined when plants were
SIII/O-11 co-inoculated with F. mosseae that resulted in increased
nutrient pool, decreased reactive oxygen species (ROS)
Zinc-Funneliformis mosseae generation via enhanced production of GSH and PCs,
Composite Application Attenuates ultimately improving growth. These findings suggested
the integrated application of Zn and AM as a promising
Cadmium Toxicity in Pigeonpea via management strategy to ameliorate Cd toxicity in
Modulating ROS Generation and pigeonpea.
Enhanced Production of Glutathione Key words: Arbuscular mycorrhiza, Cadmium, Glutathione,
Pigeonpea, Phytochelatins, Zinc.
and Phytochelatins
Harmanjit Kaur1* and Neera Garg2
Department of Botany, Akal University, Talwandi Sabo, Prospectives of Trichoderma sp. in
District: Bathinda, Punjab-151302, INDIA; 2Department of Amelioration of Arsenic Induced
Botany, Panjab University, Chandigarh-160014, INDIA, Email:
harmanjit.garcha@yahoo.com, garg_neera@yahoo.com Phyto-Toxicity
Cadmium (Cd) is a widespread toxic trace Pratibha Tripathi 1,2 *, R.D. Tripathi 2 and Alok
element, non-essential for plant growth and is found in Kalra1
agricultural soils due to various anthropogenic activities.
However, Zinc (Zn) is an essential micronutrient at CSIR-Central Institute of Medicinal and Aromatic Plants,
low concentrations but toxic to plants at higher Lucknow-226001, INDIA; 2CSIR-National Botanical
Research Institute, Lucknow-226001, INDIA, Email:
concentrations along with Cd. Zn interferes with Cd
transport by competing with Cd for binding sites of a
common transport protein on root plasma membrane. Arsenic (As), a toxic metalloid, is a food chain
Arbuscular mycorrhizal (AM) symbiosis is considered contaminant and is threat to all living forms. Irrigation
a potential biotechnological approach for increasing with As-contaminated groundwater has increased As
plant tolerance to heavy metal contamination. accumulation in agricultural soils which is a potential
Composite application of excess Zn and AM fungi can health risk for humans. Plant-microbe interaction is a
increase plant resistance to Cd toxicity, nevertheless, promising eco-friendly strategy for conventional and

Abstracts 27-30 November, 2018, NBRI, Lucknow 81

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

organic agriculture. Microorganisms have developed growth and development of vegetable and ornamental
different mechanisms to transform toxic forms of As plants. Use of Trichoderma spp. as bio control agents
to less toxic forms. In present study, role of As tolerant as well as growth promoters in agricultural systems
Trichoderma reesei NBRI 0716 in countering the As has been identified as an effective methodology to
induced stress in chickpea (Cicer arietinum) plants minimize the use of agrochemicals. Use of problematic
was studied. Arsenic exposure significantly hampered invasive plants together with agricultural wastes as
the growth, uptake of mineral nutrients and antioxidant organic materials facilitates proper recycling under
activity in chickpea resulting in poor pod setting and sustainable agriculture. Three Trichoderma spp. (T.
limited seed yield. Inoculation of chickpea with T. reesei virens, T. harzianum and T. asperullum) were
significantly promoted plant growth, yield and mineral selected and mass produced separately, using 250 g of
nutrients both in absence and presence of As. sawdust as the carrier material. Four different potting
Trichoderma inoculation also helps in restoration of media (coir + invasive plants + poultry manure with
stem anomalies like reduced trichome turgidity and rice husk; T-1, coir + invasive plants; T-2, coir +
density, deformed sclerenchymatous and compost; T-3 and compost + field soil; T-4) were
collenchymatous cells induced by As stress. prepared. Next, all the potting media were divided into
Upregulation of drought responsive genes, enhanced two parts and one set was treated with Trichoderma
proline content and shrinked cortex cells in presence spp. while the other set was used as controls, without
of As were modulated by T. reesei. In addition, adding Trichoderma. Physical and chemical properties
inoculation of T. reesei lead to three fold reduction of of the four potting media were analyzed following
inorganic As and enhanced dimethylarsinic acid and standard methods. Effect of Trichoderma amended
mono-methylarsonic acid content in soil and seed as potting media on plant growth was evaluated using
well, indicated that T. reesei probably has a role in Abelmoschus esculentus by measuring several growth
methylation of As as the possible mechanism for parameters (plant height, number of flowers and fruits
maneuvering As stress in chickpea. Therefore, yield) and disease incidence under greenhouse
bioremediation and PGP potential of T. reesei makes it conditions. Of the four potting media, T-1 exhibited
a promising bio-inoculant for application in As affected optimum physical properties i e; porosity (45.2%), bulk
sites and production of safe farm produce. density (0.34 gcm-3) and water saturation capacity
Key words: Arsenic, Bioremediation, Chickpea, Trichoderma, (52.4%); as well as chemical properties i.e. pH (6.8),
Stress. EC (0.89 ds m-1), organic carbon (17.2%), N (1.35%),
P2O5 (1.23%), K2O (1.48%) and C/N ratio (15.8). The
SIII/O-13 highest mean plant height (85.8±2.5 cm), number of
flowers per plant (18±2) and fruits yield (125.5±3.5 g)
Formulation of an Organic Potting were observed in plants treated T-1 medium with
Medium Incorporated with Trichoderma amendments. A. esculentus planted
without Trichoderma treatment in T-4 showed
Trichoderma spp. to Enhance Growth significantly lower (p<0.05) growth than T-1 and T-3
and Disease Suppression of media. A. esculentus grown in Trichoderma amended
T-1 medium showed a significantly lower (p<0.05)
Abelmoschus esculentus disease incidence (5%) compared with untreated
controls. Based on the results (T-1) was selected as
N. Hewavitharana*, S.D.P. Kannangara, L.R.
most effective medium and was prepared as a brick to
Jayasekera and S.P. Senanayake
facilitate the easy handling as well as for and
Department of Botany, Faculty of Science, University of commercialization of the product in order to introduce
Kelaniya, SRI LANKA, Email: nalakahewavitharana to both local and export markets.
Key words: Agriculture, Invasive plants, Coir dust,
Organic potting media is an important source for Trichoderma, Abelmoschus esculentus.

82 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)


Does Conversion of Forest to Reduced Arsenic Accumulation in Rice

Cropland is a Sufficient Cause to Grain by Heterologous Expression of
Make Over Soil Microbes? An Phytochelatin Synthase of
Important Discussion Ceratophyllum demersum, CdPCS1
Meenakshi Kushwaha1,2,*, Naina Marwa2,3, Vivek Manju Shri1,2*, R.D. Tripathi1,3, Prabodh Kumar
Pandey1,2 and Nandita Singh 1,2* Trivedi1,3 and Debasis Chakrabarty1,3
1 1
Academy of Scientific and Innovative Research (AcSIR), AcSIR-Academy of Scientific & Innovative Research, CSIR-
CSIR-National Botanical Research Institute (CSIR-NBRI) National Botanical Research Institute, Rana Pratap Marg,
Campus, Rana Pratap Marg, Lucknow-226001, INDIA; 2Plant Lucknow-226001, INDIA; 2Present address: School of Life
Ecology and Environmental Sciences Division, CSIR- Sciences, Central University of Gujarat, Gandhinagar-382030,
National Botanical Research Institute, Lucknow 226001, INDIA; 3CSIR-National Botanical Research Institute, Rana
INDIA; 3Department of Botany, University of Lucknow, Pratap Marg, Lucknow-226001, INDIA, Email:
Luknow-226007, INDIA, Email: meena.abc7@gmail.com manjubiochem20@gmail.com
In the race of modernization, deforestation and Rice (Oryza sativa L.) is amidst the great
agriculture intensification has become the top threat to essential food crop that offers a staple food for most
the nature worldwide. As a result of deforestation, soil of the world’s populace. Arsenic (As) is a carcinogenic
qualities get modified and subsequently affect the soil heavy metal, which harms human health. In Asian
microbiota in different ways. Bacteria and fungi, the countries, a major source of human As-intake is rice
key soil microbes are the drivers of the soil biology and grains and; contamination of paddy soils by As and
biogeochemistry. They are drastically affected by the accumulation of As in rice grains is one of the serious
deforestation activities in various aspects. Previous agricultural issue. The predominant model for plant
research articles described about these aspects in their detoxification of heavy metals is complexation of heavy
own ways; for example, loss, gain, modification or no metals with phytochelatins (PCs), synthesized non-
effect on their diversity and composition separately. translationally by PC synthase (PCS) and
Some researchers also focused that these different compartmentalized in vacuoles. In this study, in order
aspects of bacterial and fungal diversity modification to restrict As in the rice roots as a detoxification
following deforestation are also influenced by the mechanism, a transgenic approach has been followed
environmental factors like plant community, edaphic through expression of phytochelatin synthase,
factors (pH, water holding capacity, and soil nutrients), CdPCS1 , from Ceratophyllum demersum, an aquatic
climatic conditions (micro and macro) and man-made As-accumulator plant. CdPCS1 expressing rice
factors like fertilizer, cropping pattern, and crop plant transgenic lines showed marked increase in PCS
type. The study about the combined effect of all activity and enhanced synthesis of PCs in comparison
environmental and man-made factors may result in the to non-transgenic plant. Transgenic lines showed
more valuable information to hit upon the knowledge enhanced accumulation of As in root and shoot thereby
of microbial alteration following deforestation and restricting its accumulation in grain. This enhanced metal
cropping practices in a single platform. accumulation potential of transgenic lines was
Key words: Deforestation, Microbial community, positively correlated to the content of PCs, which also
Environmental, Bacteria, Fungi increased several-fold higher in transgenic lines. The
higher level of PCs in transgenic plants relative to non-
transgenic presumably allowed sequestering and
detoxification of higher amounts of As in roots and
shoots, thereby restricting its accumulation in grain.
Key words: Rice, Arsenic, Phytochelatin, Transgenic,
Ceratophyllum demersum.

Abstracts 27-30 November, 2018, NBRI, Lucknow 83

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SIII/O-16 modulation of amino acids and phyochelatins in rice

Selenium Linked Arsenic Tolerance Key word: Arsenate, Amino acids, Antioxidants, Selenate,
Entails Induction of Phytochelatins ROS staining, Phytochelatins.
and Amino Acids and Promotes SIII/O-17
Reduction of Arsenic Phytotoxicity
and Uptake in Rice Phylogenetic Analysis of HKT
Homologs in Grasses Identified
Preeti Tripathi 1,2 *, Reshu Chauhan 1 , Surabhi Conserved Domains and Potential
Awasthi1 and R.D. Tripathi1
Functional Sites
CSIR-National Botanical Research Institute, Council of
Scientific and Industrial Research, Lucknow-226001, INDIA;
2 P.R. Weerasinghe 1* , M.H.M.M.N. Mapa 2 and
Ministry of Environment, Forest and Climate Change, New
Delhi-110003, INDIA, Email: preeti71985@gmail.com H.A.C.K. Ariyarathna3
Coconut Research Institute, Bandirippuwa Estate, Lunuwila,
Exposure of population through consumption of
Sri LANKA; 2Sara Bhumi Lanka Bio Products (Pvt.) Ltd.,
arsenic (As) tainted rice is a major problem over the
Dambarawa, Pilawala, SRI LANKA; 3Department of Botany,
world especially in South and South East Asia. To Faculty of Science, University of Peradeniya, SRI LANKA
overcome the As induced phytotoxicity in rice, selenium Email:pabasaraweerasinghe@yahoo.com, nisansala.mapa1
(Se) was supplied as selanate (SeVI) during arsenate @gmail.com, ckariyarathna@yahoo.com
(AsV) stress in rice during hydroponics. Se(VI)
Salinity of soil is a global issue affecting more
addition exhibited markedly (p<0.05) reduced level of
than 40% of the irrigated lands worldwide that causes
As and oxidative stress in roots and shoots as well as
adverse effects on crop production. The High Affinity
induced growth suggesting its beneficial role either in
K+ Transporters (HKT) play a key role in salinity
the presence of As. For the first time, different species
tolerance in plants by regulating ion transport in tissues
of phytochelatins (PC2, PC3 and PC4) were analyzed
and therefore can be used as targets to improve salinity
during Se(VI) and As(V) interaction. Total PCs in roots
tolerance. This study utilizes available sequence
were in the order of control<Se<As<As+Se suggesting
information and plant genome databases to predict the
the role of thiols for As tolerance and detoxification
evolution of HKT genes and the potential functional
during As/Se interaction. The phytochelatin synthase
sites of the respective transporters. Eight HKT genes
(PCS) activity respond according to PCs contents along
previously identified in rice were used as queries to
with serine acetyl transferase (SAT), cysteine synthase
predict 51 orthologous HKTs from six species in the
(CS), ã-glutamyl cysteine synthase (ã-ECS) and ã-
Graminae family using genome databases and BLAST
glutathione transferase (ã-GT) exhibiting their maximum
analysis. Rice genes OsHKT1;1 and OsHKT1;4 were
activities at Se(VI) added As(V) stressed plants.
adjacent gene pairs on chromosome 1and similarly
Arsenic distorted the essential amino acids (EAAs),
OsHKT2;1 and OsHKT2;4 are adjacently located on
however Se(IV) improved their (isoleucine, lysine,
chromosome 6 providing evidence for ancient tandem
leucine, threonine and phenylalanine) level during As(V)
gene duplication events. Similarly, in each species
stress. Some stress responsive non-essential amino
studied, orthologs of OsHKT1;1 and OsHKT1;4
acids (proline, cysteine, glutamic acid and glycine)
OsHKT2;1 and OsHKT2;4 were found as neighboring
increased during Se(VI) and As(V) exposure and some
gene pairs forming syntenic blocks with rice.
(arginine, serine and aspartic acid) recovered to the
Phylogenetic trees constructed using protein sequences
level of control implying the role of Se(VI) for oxidative
with 1000 bootstrap replications predicted three main
stress removal, which probably leading to lower
clusters of HKT transporters (bootstrap > 65). Each
peroxidation of amino acids due to lesser free radicals.
of these main clusters were further separated in to
Thus, Se(VI) imparts As(V) tolerance through
two sub clusters. Thereby main cluster 1 included the

84 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

orthologs of rice OsHKT1;4 and OsHKT1;5. Orthologs property in the area of biotechnology helps to accelerate
of OsHKT1;1 and OsHKT 1;3 were included in the economic and social development for a developing
main cluster II and the third main cluster included country like India. However, there are IP concerns in
orthologs of OsHKT2;1 and OsHKT2;4. Interestingly developing countries and a need for greater protection
the genes that exist as neighboring pairs were clustered and access. Isolation of live organisms e.g. bacteria
in different main clusters indicating that tandem gene etc. simply from their habitat were not eligible for
duplication and subsequent divergence could be patents. Biotechnology and genetic engineering makes
potential mechanism in the evolution of HKTs. it possible to include such biotechnological products.
However, G block predictions identified highly Also the patenting of life forms is unlike mechanical
conserved sequence fragments among the HKTs, inventions and thus poses ethical issues in biotechnology.
specifically in the P-loop domains identifying potential Key words: Patent, Innovation, IPR, Intellectual Property,
functional sites. These findings provide a basis to GI, Economy.
illuminate on the potential functional sites on HKTs.
Key words: Salinity, Granimae, Genome databases, BLAST, SIII/O-19
G block.
Analysis of HKT Homologs in Grasses
SIII/O-18 by Comparative Genomics and Protein
Intellectual Property Rights and Structure Prediction
Biotechnology Issues M.H.M.M.N. Mapa1,2 *, P.R. Weerasinghe1,3 and
H.A.C.K. Ariyarathna1
Saurabh Yadav
Department of Botany, University of Peradeniya, Peradeniya,
Department of Biotechnology, Hemvati Nandan Bahuguna SRI LANKA; 2Sara Bhumi Lanka Bio Products (Pvt.) Ltd,
Garhwal (Central) University, Srinagar Garhwal-246174, Dambrawa, Pilawala, SRI LANKA; 3Coconut Research
Uttarakhand, INDIA, Email: saurabhyadav40@rediffmail.com Institute, Bandirippuwa Estate, Lunuwila, SRI LANKA
Everyone desires to acquire a property, mostly Email:nisansala.mapa1@gmail.com, pabasaraweerasinghe@
tangible ones. But intangible property have also much yahoo.com, ckariyarathna@yahoo.com
value, since it is having long term effect. One of the
Soil salinity is a major abiotic stress that incurs
intangible property is Intellectual property. Intellectual
economic losses in crops. High affinity K+ transporters
property is generated by the application of human
(HKTs) are a class of integral membrane proteins
intellect. ‘Intellectual property’ is the dynamic area
(IMPs) involved in Na+ transport and some members
due to the emergence of new knowledge and
of this group are candidate proteins associated with
technologies. The ‘brick and mortar economy’ deals
salinity tolerance. This analysis aims in-sillico prediction
with all the properties such as land, house etc. The
of potential functional sites in this group of proteins
‘Knowledge economy’ is the focus of the worldwide
based on evolutionary significance of the different
economy, in current scenario. IP protection is mainly
domains. Fifty-one orthologous protein sequences from
bypatents, copyrights, geographical indications,
Brachypodium distachyon, Sorghum bicolour
trademarks, trade secrets, know-how etc.
(Sorghum), Setaria italica, Zea mays (Corn),
‘Biotechnology’ deals with living forms and inventions
Hordeum vulgare (Barley), and Triticum aestivum
related to biotechnology must be dealt with extra care
(Wheat), were retrieved by quarrying genome
to avoid legal issues and needs to be protected by
databases using eight previously known rice (Oryza
intellectual property rights. Inventions related to
sativa) HKT proteins as the queries. The conserved
biotechnology seems to be promising for industrial
domains in the proteins were identified by G Blocks
productivity. The legal battle related to turmeric, basmati
(www.idtdna.com) prediction. Protein 3D structures
rice and neem are examples of strict IPR laws. Non-
were obtained by homologous protein prediction by
patentable biotechnology inventions and also legal issues
PYRE2 and analyzed by PYMOL viewer. The
like ethics, morality etc. Protecting the intellectual

Abstracts 27-30 November, 2018, NBRI, Lucknow 85

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

conserved domains were studied in the 3D structures. use a set of Arabidopsis GUS mutation detector lines
Three highly conserved domains were identified where to study the impact of anthropogenic activities on
group mean pair wise distance was 0.451. Based on genome stability. Our research has revealed that the
structure predictions these domains were found in the two crop protection methods - genetic transformation
first, second and third Trans- membrane domain and and pesticide application, have opposite impact on
the P loop regions. These three domains predict the somatic mutations like homologous recombinations,
potential functional sites in HKTs that can be further frame-shift and point mutations. Both the stresses
studied to illuminate on mechanisms of iron transport caused epigenetic changes, as analysed by methylation-
and to identify functional variants among the proteins sensitive restriction digestion PCR, in the promoters of
to enable utilizing these genes in improvement of salinity certain DNA repair genes involved in recombination,
tolerance in plants specially the important cereal crops. base-excision repair or nucleotide-excision repair
Key words: Salinity tolerance, Homology prediction, Protein pathways. To the best of our knowledge, there are no
3-D structure, Functional domains. previous studies on such an epigenetic analysis of DNA
repair system. Our approach provides an excellent
SIII/O-20 platform to study transgenerational memory of other
environmental hazards as well.
Molecular Basis of How Plants can be Key words: Transgenerational memory, Epigenetics,
Used as a Potential Detector System Mutation, Methylation, DNA repair.
to Analyse Environmental Hazards SIII/O-21
Jasmine M. Shah*, J. Sijila and T.J. Joyous Effect of Fly Ash on Growth of
Department of Plant Science, Central University Kerala, Cellulolytic Bacteria
Kasaragod, Kerala-671316, INDIA, Email: jasmine@cukerala.
ac.in, sijila.janardhanan@gmail. com, joyoustj@gmail.com
M.S. Dhanya* and P. Ashoka Chakravarthy
Plants are sessile organisms and are forced to Department of Environmental Sciences and Technology,
face the injustices imposed by environment and Central University of Punjab, Bathinda-151001, Punjab,
anthropogenic activities. Stress perceived by any INDIA, Email: dhanyasubramanian@gmail.com,
organism, is often evidenced by a physiological change. ashokachakravarthy987@gmail.com
Changes when occur in animals, often turn out to be
The cellulose is the most abundant material on
lethal whereas, plants can still thrive with an
the earth. This is the major component of organic wastes
abnormality. For example, sterility, a phenotype which
such as kitchen waste and requires waste management.
brings an end to a generation in animals, can be easily
The cellulolytic bacteria play an important role in
ignored by a vegetatively propagated flora. Phenotype
maintaining the ecosystem balance by means of
is nothing but manifestation of the genotype. Recent
decomposition. The hydrolysis depends on cellulolytic
research has revealed that not only genetic, but also
microbes which is overall rate limiting step in
epigenetic changes reflect into the making of a
composting and anaerobic digestion. The fly ash is an
phenotype. DNA, the genetic basis of life, is highly
inorganic waste that is generated from thermal power
conserved across the life forms. More importantly, the
plants as a major pollutant. The disposal of food waste
mechanisms of DNA replication, repair and epigenetic
and fly ash are of great concern. The focus of the
regulation are highly conserved across the eukaryotic
study was to evaluate the effect of fly ash on the growth
world, from yeast to plants to mammals. Hence, plants
of cellulolytic bacteria involved inorganic waste
offer an excellent system to monitor the molecular
digestion. The fly ash addition to food wastes enhanced
impact of environmental hazards, both genetic and
the cellulase activity and biomass growth of cellulolytic
epigenetic, simply because of ethical reasons and easy
bacteria. The fly ash addition to rice wastes increased
handling. Our group works on genomic stability,
cellulase production by cellulolytic bacteria than roti
expression and epigenetic regulation of DNA repair
wastes. The fly ash improved the manorial efficiency
and transgenerational memory of plant genome. We

86 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

of food wastes and helps in production of nutrient rich approach in order to enhance the beneficial effects of
manure that can be used for plant growth. Si in chickpea. Conclusively, these results support usage
Key words: Fly ash, Cellulolytic bacteria, Rice waste, Roti of Si along with AM as potent candidates for alleviating
wastes, Sustainable waste management. salt-induced osmotic stress.
Key words: Chickpea, Mechanism(s), Salinity, Silicon,
SIII/O-22 Proline, Soluble sugars.

Silicon Fertilization and Arbuscular SIII/O-23

Mycorrhizal Fungi Alleviate Salinity
Study on Behaviour of Tylophora
by Regulating Proline and
indica in vivo and in vitro with Special
Carbohydrate Metabolism in Cicer
Reference to its Fungicidal Activity
arietinum L.
Suman Das1,2*, Shibasis Mukharjee1, Alok Hazra1
Purnima Bhandari1 * and Neera Garg2 and Maitreyi Banerjee3
MCM DAV College for Women, Chandigarh, INDIA; 1
Department of Agricultural Biotechnology, Ramakrishna
Department of Botany, Panjab University, Chandigarh- Mission Vivekananda Educational and Research Institute,
160014, INDIA, Email: purnima3320@gmail.com, Narendrapur, Kolkata-700103, INDIA; 2Department of
garg_neera@yahoo.com Handloom Handicrafts and Sericulture, Sericulture Training
Soil salinity is recognised as a major threat to Institute, Tripura, INDIA; 3West Bengal State Council of
legume productivity by causing osmotic and ionic stress. Science and Technology, Kolkata, INDIA, Email:
Chickpea (Cicer arietinum L.) is considered highly
sensitive to salt stress. Various studies have highlighted Tylophora indica is a medicinal plant which has
the promising role of silicon (Si) and arbuscular established its importance in the recent years as
mycorrhizal (AM) fungi in alleviating salt induced observed from the increasing number of reports &
osmotic stress via regulating osmolyteaccrual especially diverse types of study. A number of studies have been
proline (Pro) as well as soluble sugars (SS). However, made in vitro but no study has so far been made to
the exact mechanism(s) via which both modulate solute compare their performance in vivo and in vitro. The
levels is still lacking. Moreover, very few studies have in vivo and in vitro raised plants were compared with
highlighted the promising role of Si in regulating Pro reference to their morphological, phytochemical
and SS biosynthesis in otherwise low Si accumulating characters as well as their fungicidal capacity. Micro
legumes. To address this issue, pot trials were propagation was done taking nodal explants & best
conducted in two differentially tolerant chickpea obtained using BAP 3mg l-1 (both establishment of
genotypes which were supplemented with NaCl (0, 60, explants and their multiplication). Callusing was best
80 and 100 mM), Si (K2SiO3 - 0 and 4 mM), with or obtained with 2,4 D and Kinetin and grown from leaves.
without Funneliformis mosseae inoculations. Callusing was frequently observed at the base of the
Exogenous Si and/or AM fungus counteracted the nodal explants spontaneously. Rooting also was often
negative effects of osmotic stress by significantly spontaneous after growth of the plants were complete.
reducing Na+ uptake and upgrading the activities of This probably indicates the higher level of endogenous
enzyme involved in Pro and SS biosynthesis, ultimately, auxin within the plants. The phytochemical studies were
leading to better growth performance under salinity. made with respect to a secondary metabolite
Comparatively, mycorrhizal inoculations were more (Flavonoid). The flavonoid content was higher in case
efficient in boosting P5CS, OAT and GDH activities of plants raised in vitro than those in vivo. Also, the
with a concomitant decline in ProDH. Benefits of Si callus showed higher amount of flavonoid than that
were directed more towards accumulation of SS that found in vivo plants. Extract of the plants was prepared
led to improved shoot growth. Mycorrhizal inoculations and their performances were evaluated in respect to
were used along with Si nutrition as a multidisciplinary antifungal activities against a common fungal pathogen

Abstracts 27-30 November, 2018, NBRI, Lucknow 87

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

(Aspergilus niger) and it was found that the extract in PGPR and its significance as an alternative of
of the plants grown in vivo was much less effective chemical fertilizers towards sustainable agriculture.
as an antifungal agent than the extract obtained from Key words: Crop yield, Plant growth promoting rhizobacteria
those grown in vitro by the Minimum Inhibitory (PGPR), Sustainable agriculture.
Concentration (MIC) test. (Note: MS - Murashige and
Skoog, PGR – Plant growth regulator, BAP – Benzyl SIII/P-2
amino purine, 2.4 D – 2-4 dichloro phenoxy acetic acid)
Key words: Nodal explants, MS, Callus, Antifungal activity, Indirect Organogenesis from Leaf
Flavonoids, MIC. Segments of Solanum lycopersicum
SIII/P-1 Poonam Chaudhary 1 *, Balwinder Singh 2 and
Avinash Kaur Nagpal1
Plant Growth Promoting 1
Department of Botanical and Environmental Sciences, Guru
Rhizobacteria: Prospects towards Nanak Dev University, Amritsar-143005, Punjab, INDIA;
Sustainable Agriculture 2
Post Graduate Department of Biotechnology, Khalsa
College, Amritsar, INDIA, Email: poonamchaudhary73@
Ashmita Ghosh 1 , Mrinal Ray 1 , Debnirmalya yahoo.com, bbs171@rediffmail.com, avnagpal@
Gangopadhyay1* and Suman Das2
Department of Sericulture, Raiganj University, Raiganj, Uttar
Series of experiments were performed to test the
Dinajpur-733134, West Bengal, INDIA; 2Deptartment of effect of different concentrations of 2,4-
Handloom Handicrafts and Sericulture, Sericulture Training dichlorophenoxy acetic acid (0.1-1.0 mg l-1) alone and
Institute, Tripura, INDIA, Email: deb_ganguly2003@ in combination with kinetin (0.5 mg l-1) on indirect
yahoo.com organogenesis response from leaf segments of
Solanum lycopersicum. Callus initiation was observed
The chemical fertilizers and pesticides not only
on leaf segments in all the treatments from 7-10 days
create environmental and human health problems but
after inoculation. Maximum callus induction (90%) was
also greatly affect the soil health. It has been reported
observed on MS medium supplemented with 0.1 mg l-
that regular use of chemical fertilizers brings down the 1
2,4-dichlorophenoxy acetic acid and 0.5 mg l-1 kinetin.
productive capacity of the soil. Therefore, the soil with
The in vitro response of explants for callus induction
poor productivity often results in low profit particularly
was recorded on 28th day after inoculation and it was
for the marginal farmers who viciously tied to take loans
found that the calli were white and compact. The calli
to meet the rising cost of agrochemicals. Of late, the
were cut into small pieces and inoculated onto
importance of plant growth promoting rhizobacteria
regeneration medium [MS medium containing different
(PGPR) attracted the attention of the researchers.
concentrations of 6-benzylaminopurine (0.1-2.0 mg l-
Various studies revealed that PGPR helps in increasing 1
)]. Maximum shoot regeneration (76%) was observed
crop yield by improving soil fertility, accelerating plant
on medium supplemented with 0.25 mg l -1 6-
growth and suppressing phytopathogens etc. The
benzylaminopurine. Shoots were healthy and achieved
physiology of plant gets affected by PGPR through
the length of 6.18±1.75 cm within 4 weeks. Shoot
various mechanisms like phytostimulation (via
regenerated on MS medium containing 6-
production of gibberellins, indole acetic acid, ethylene
benzylaminopurine (0.1-0.75 mg l-1) was shown to root
and cytokinins), biofertilization (through phosphate
as well without the addition of auxins. This study reveals
solubilization, biological fixation of atmospheric nitrogen,
that transfer of calli (raised on medium containing 0.1
exopolysaccharides production and siderophores
mg l-1 2,4-dichlorophenoxy acetic acid and 0.5 mg l-1
production); and bio-defense mechanisms (via systemic
kinetin) to MS medium with 6-benzylaminopurine 0.25
resistance, competition for nutrient and space,
mg l-1 was the best option to obtain complete plantlets.
production of lytic enzymes, antibiotics, hydrogen
This experiment would be useful to create somaclonal
cyanide) etc. The study illustrates the latest advances

88 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

variations and also for utilizing transgenic approaches indicator lichen species can be used for monitoring of
to improve tomato varieties. environmental quality in the study area.
Key words: Solanum lycopersicum, Somaclonal variations, Key words: Bio-indicator, Heavy metal, Nitrophile, Pollutant,
MS medium. Toxi-tolerant.


Notes on Bio-Indicator Lichens Three Auxin Response Factors

Enumerated from Sikandra Hill of Promote Hypocotyl Elongation
North West Himalaya
Ashish Chaturvedi
Monika Thakur* and Hem Chander Department of Pathology, King George’s Medical University,
Lucknow-226007, INDIA, Email: ashishchaturvedi.jr.sc
Division Botany, Department of Bio-Sciences, Career Point
University Hamirpur (H.P.), INDIA, Email: thakurmonika382
@gmail.com, hemchander78@gmail.com The hormone auxin regulates growth largely by
Lichens are bio-indicator of air pollution, especially affecting gene expression. By studying mutants
sulfur dioxide pollution. They are inexpensive to use in deficient in Auxin Response transcription Factors
evaluating air pollution and are able to react to wide (ARFs), we have identified three ARF proteins that
range of air pollutants over a period of time as compared are required for auxin-responsive hypocotyl elongation.
with other physical/chemical monitors. The quality of Plants deficient in these factors have reduced response
environment in a particular area can be assessed either to environmental conditions that increase auxin levels,
by monitoring changes in lichen community or through including far-red-enriched light and high temperature.
monitoring their physiological changes. The toxic Despite having decreased auxin response, the ARF-
elemental pollutants and radioactive metals bind with deficient plants responded to brassinosteroid and
mycobiont and concentrate over time. During the lichen gibberellin, indicating that different hormones can act
floristic studies, three hundred specimens of lichens partially independently. Aux/IAA proteins, encoded by
were collected from Sikandra hill, which is situated in IAA genes, interact with ARF proteins to repress auxin
Shivalik zone of North West Himalaya and is located response. Silencing expression of multiple IAA genes
in district Mandi of Himachal Pradesh (India). These increased hypocotyl elongation, suggesting that Aux/
specimens were then investigated morpho-chemo- IAA proteins modulate ARF activity in hypocotyls in a
taxonomically and thirty species of lichens have been potential negative feedback loop.
identified so far. Out of these, ten species of lichens Key words: Auxin, ARFs, Hypocotyls, Negative.
(viz. Candelaria concolor (Dicks.) Arnold,
Dermatocarpon vellereum Zschacke, Heterodermia SIII/P-5
pseudospeciosa (Kurok.) W.L. Culb, Lecanora
chlarotera Nyl, Parmotrema praesorediosum (Nyl.) Systematic Identification and Analysis
Hale, Parmotrema tinctorum (Despr. ex Nyl.) Hale, of Lysine Succinylation in Strawberry
Phaeophyscia hispidula (Ach.) Essl, Physcia
stellaris (L.) Nyl., Punctelia subrudecta (Nyl.) Krog,
Pyxine subcinerea Stirt) act as bio-indicator.
Ashish Chaturvedi
Candelaria concolor is a nitrophile and act as indicator
of nitrogen pollution, whereas, Punctelia subrudectais Department of Pathology, King George’s Medical University,
nitrogen tolerant. Heterodermia pseudospeciosa Lucknow-226007, INDIA, Email: ashishchaturvedi.jr.sc
belong to physcioid lichen community and is toxi-tolerant
species. The other seven lichen species act as bio- The various post-translational modifications
indicator of heavy metal air pollutants (iron, chromium, of plant  proteins have  important  regulatory  roles  in
copper, zinc, lead and nickel. These potential bio- development. We therefore examined various

Abstracts 27-30 November, 2018, NBRI, Lucknow 89

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

modified proteins from strawberry stigmata and found callus 1079 proteins, while in nonembryogenic callus

that succinylation of lysine residues was the most 982 were exclusively identified. These proteins were
abundant type of modification. We then found to be associated with varied cellular functions.
subjected proteins from  strawberry  stigmata  to  an The most of these proteins function in different stress
efficient enrichment method for succinylated peptides responses and metabolic processes. More than seventy
and identified 200 uniquely succinylated lysines in 116 stress related proteins were observed exclusively in
proteins. A bioinformatics analysis revealed that these embryogenic callus including ascorbate peroxidase,
proteins are involved in important biological processes, glutathione S transferase, superoxide dismutase,
including stress responses, vesicular transport, and catalase, alkylhydro peroxidase, pyridine nucleotide
energy metabolism. Proteomics, combined with disulphideoxidoreductase, SOD Fe-N domain
immunoprecipitation and immunoblotting, revealed an containing protein, thioredoxinreductase. The role of
obvious increase in succinylation of the assembly stress/proteins was discussed in this article.
polypeptide 2 (AP2) and clathrin from 0.5 to 2 h after Key words: Somatic embryogenesis, Stress proteins,
pollination, suggesting that succinylation is involved in Catharanthus roseus, LC-MS, Embryogenic proteins.
the recognition of pollen-stigma signaling substances
and vesicular transport. These results suggest that AP2/ SIII/P-7
clathrin-mediated vesicular transport processes are
regulated by lysine succinylation during pollen Lipase Catalyzed Biodiesel Production:
recognition. A Review
Key words: Succinylation, Proteins, Stigmata, Polypeptide
2-AP2. Chandra Bhan* and Jiwan Singh
Department of Environmental Science, Babasaheb Bhimrao
Ambedkar University, Lucknow-226025, INDIA, Email:
evs.bhan@gmail.com, jiwansingh95@gmail.com
Proteomic Study of Somatic
The rapid growing population, fast industrialization
Embryogenesis (A Tool for Rapid
and modernization have increased the energy demand
Plant Development) in Catharanthus in developing countries. The conventional energy
roseus sources are limited in nature and about to run off very
fast. In this condition, alternative fuels mandatory to
Basit Gulzar* and Abdul Mujib get rid of energy crisis. So, biodiesel is the better
alternative fuel of the petro-diesel fuel Biodiesel is
Department of Botany, Jamia Hamdard, New Delhi-110062,
INDIA, Email: basit.gulzar786@gmail.com; amujib3@
biodegradable and produces fewer toxic pollutants
yahoo.co.in and greenhouse gases than petroleum diesel. Biodiesel
have the potential to replace diesel in vehicle engines.
Somatic embryogenesis (SE), an in-vitro, Biodiesel produce by transesterification of vegetable
multistep and a model technique used for the study of oil, fat and waste cooking oil with alcohol in the presence
plant development under the stressful micro of chemical catalyst. Chemical catalyst such as sodium
environment and used to develop embryos/plants from hydroxide, potassium hydroxide, sulfuric acid, sodium
single or a group of cells. The objective of the study ethoxide and potassium ethoxideare used in biodiesel
was to identify the somatic embryogenesis related production. But some disadvantages of chemical
proteins via label free liquid chromatography-mass catalysts, such as require high reaction temperature,
spectroscopy (LC-MS) in Catharanthus roseus. A difficult purification of glycerol. Alkali catalysts are
comparative proteomic analysis of non embryogenic inexpensive and preferred but activity is less. Sulfuric
and embryogenic calli of C. roseus was pursued. A acid is used as catalyst in biodiesel, it leads to corrosion
total of 3573 proteins were identified in both non of the reactor and large amount of wastewater
embryogenic and embryogenic calli. 1511 proteins were generated during neutralization of mineral acid. Acid-
found to be common in both the calli. In embryogenic catalyzed transesterification reaction needs higher

90 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

alcohol-to-oil molar ratio. So, these chemical catalysts such as Pseudomonas sp., Aspergillus versicolor,
may replace by lipase enzyme. Lipase have the capacity Saccharomyces cerevisiae, Cunninghamellla
to performed transesterification reaction. Lipase elegans, Microbacterium profundi, Cladosporium
produced from number of microorganisms such as are widely used (alone or in combination) for
bacteria, fungi etc. Lipase from fruit waste also used bioremediation of heavy metals from metal
in biodiesel production. The use of lipase will minimize contaminated sites. Moreover, associations of plant
the chemical catalysts. So, the enzymatic based roots with soil microorganisms such as rhizobium and
biodiesel production is the emerging research area for arbuscular mycorrhizal fungi have proved to be a
grow toward green chemistry and sustainable promising strategy for imparting metal stress tolerance
development. in crop plants for sustainable agriculture. In this review
Key words: Energy, Biodiesel, Transesterification, Catalyst, poster, we explore the difference mechanisms
Lipase. undertaken by plants and microbes in response to heavy
metal stresses.
SIII/P-8 Key words: Heavy metals, Bioremediation, Rhizobium,
Arbuscular mycorrhizal fungi.
Heavy Metal(loid) Stresses in Plants
and their Detoxification by Soil SIII/P-9
Microorganisms Trichoderma Mediated Modulations
Ipsa Gupta* and Neera Garg of Chickpea Rhizospheric Activities in
Department of Botany, Panjab University, Chandigarh- Presence of Organic Amendments and
160014, INDIA, Email: garg_neera@gmail.com, ipsagupta Inorganic Fertilizers
Heavy metal(loid) (HMs) including arsenic, lead, Anshu*, Ashmita Tandon, Udit Uadav, Pankaj
nickel, cadmium, chromium and mercury are important Kumar Srivastava, Kumkum Mishra and Poonam
environmental pollutants that are increasing across the C. Singh
globe due to geologic and anthropogenic activities. Their CSIR-National Botanical Research Institute, Rana Pratap
presence in the soil leads to increase in oxidative stress, Marg, Lucknow-226001, INDIA, Email: anshugautam1808
degradation of plant growth cycles (including @gmail.com
embryogenesis, organogenesis), oxidation of protein, Interaction of plant growth promoting
inactivation of enzymes, DNA damage etc. Plants microorganisms (PGPMs) with plants interchanges
respond to HM toxicity in a variety of different ways signals at physical, physiological and molecular levels.
such as immobilization, exclusion, chelation and Proliferation and root colonization of PGPMs contribute
compartmentalization of the metal ions, and the in improving plant functions by manipulating the
expression of more general stress response mechanisms rhizosphere. They benefit plants through different
such as ethylene and stress proteins however, these mechanisms like nutrient enrichment and induction of
mechanisms are scarcely effective under high plant vigor and defence system. The present work aims
concentrations of metals. Several physical techniques to decipher the rhizosphere modulations by an organic
such as adsorption and chemicals like alginates, citrus acid producing Trichoderma strain (NBRI-PR5) under
pectin, flavonoids and phytic acid are being used to different fertilization regimes. Chickpea reciprocates
remove heavy metal contaminants from the thoroughly to the application of organic amendments,
environment which however are costly, mechanically inorganic fertilizers, and NBRI-PR5. The determining
complex and time consuming. Bioremediation is, factor in the study was the microbial communities
therefore, an eco-friendly and efficient method of associated with the plant rhizosphere which was
reclaiming metal(loid) contaminant environments by determined by culturable populations of heterogeneous
making use of the inherent biological mechanisms of microflora (Bacteria, fungi, and Actinomycetes) .The
microorganisms and plants. In this technology, microbes

Abstracts 27-30 November, 2018, NBRI, Lucknow 91

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

rhizospheric functions were studied by determining the LWG002611 possesses suitable biodiesel properties as
soil enzyme activities and HPLC profiles of organic per the range defined by the European biodiesel
acids in root exudates. The application of NBRI-PR5 standard EN14214 and petro-diesel standard
strain induced favourable changes in rhizosphere upon EN590:2013. The whole genome sequencing (WGS)
the application of amendments. The minimum bacterial predicted a total of 13514 genes from de novo and
CFU noticed in the NPK treatment alone (4.18 Log10 16739 genes from reference guided assembly. The
CFU g-1) confirming the inhibitory effects of chemical genome size was estimated at 65.35 Mb. Metabolic
fertilizers on rhizosphere microflora compared to the pathways including lipid metabolic pathways were
unamended control (6.71 Log10 CFU g-1). Application reconstructed and a total of 283 genes were identified
of NBRI-PR5, with organic amendments or inorganic in lipid metabolism. Multiple gene homologs were
fertilizers lower the pH of the rhizosphere causing identified in triacylglycerol (TAG) biosynthesis pathway.
inhibition of alkaline phosphatase activity in the The phylogenetic analysis from WGS revealed that the
treatments compared to the unamended control strain belongs to class Chlorophyceae and order
(0.5µgpNP.g-1 soil.h-1 ). NBRI-PR5 had refurbished Sphaeropleales. Molecular identification was carried
microbial activities to facilitate nutrient uptake in plants out by sequence of small subunit ribosomal RNA gene
rather than fix in the microbes which is demonstrated (18S rRNA) and validated through morpho-taxonomy.
by an opposite trend in DHA and protease enzyme Taken together, this first report on WGS of S.
activities in the rhizosphere of FYM and FYM+PR5 quadricauda and an insight into the lipid biosynthesis
treated plants. The conclusion of the study is that pathway could identify the targets for genetic and
NBRI-PR5 momentously regulates rhizosphere metabolic engineering to further improvement of the
activities in terms of enzymatic and microbial activities strain and useit as a sustainable source of biodiesel.
of the rhizosphere, distinct to different fertilization Key words: Secenedmus quadricauda, Biodiesel properties,
regimes by varying the enzyme activities to maximize Whole genome sequencing, Lipid biosynthesis pathway,
the utilization of available nutrients. Phylogenetic analysis.
Key words: Soil enzymes, Organic acids, Rhizosphere
remodelling, Chickpea, Trichoderma. SIII/P-11

SIII/P-10 Insilco Studies of Mango Genome

Cultivars and Development of
Draft Genome Sequencing, Functional
Analysis Tool
Gene Annotation, Reconstruction of
Lipid Metabolic Pathways and Rabia Faizan*, Tayyaba Qamar-ul-Islam ,
Comparative Evaluation of Oleaginous Muhammad Ahmed and Uzma Mehmood
Microalgae Scenedesmus quadricauda Sir Syed University of Engineering and Technology, Karachi
75300, PAKISTAN, Email: rabiatabassumkhi@gmail.com
LWG002611 Mango known as king of all fruits and fifth most
important subtropical/tropical fruit crops worldwide
Chitralekha Nag Dasgupta*, Sanjeeva Nayaka,
with the production centered in India and South-East
Kiran Toppo and Atul Kumar Singh
Asia. Recently, there has been a worldwide interest in
Algology Division, CSIR-National Botanical Research mango genomics to produce tools for marker
Institute, Lucknow-226001, INDIA, Email: chitralekha. annotations and trait information. There are no online
dasgupta@gmail.com analyzed genomic tools available for mango specially.
The oleaginous microalgae have huge potential Moreover a complete mango genomic resource was
as an alternative source of energy. The present study required for improvement in research and annotations
reveals that the high biomass (1.41±0.13 gL-1) and lipid of mango germplasm. In this paper, we have done
(404±30 mgL-1) yielding Scenedesmus quadricauda comparative analysis of four mango cultivars i.e. cv.

92 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Langra, cv. Zill, cv. Shelly and cv. Kent from Pakistan, properties through the modulation in physico-chemical
China, Israel, and Mexico respectively. There are parameters viz. pH, time, temperature, concentration
30,953-85,036 unigenes identified from RNA-Seq of cell free extract, and gold salt during biosynthesis of
datasets of mango cultivars in De-novo sequence gold nanoparticles. A varying size ranges from 2–500
assembly. The significance of this specific tools is to nm with different shapes i.e. spherical, triangular,
provide the scientific community and general public a nanoprisms, pentagon/hexagon and nanosheets were
mango genomic resource and allow the user to examine synthesized. Catalytic activity of these gold
their data against our analyzed mango genome nanoparticles were evaluated by UV-visible
databases of four cultivars (cv. Langra, cv. Zill, cv. spectroscopy followed by gas chromatography-mass
Shelly and cv. Kent). A mango web genomic resource spectrometric analysis. Spherical nanoparticles of size
MGdb, is based on 3-tier architecture, developed using 3-10 nm were showing highest catalytic activity among
Python, flat file database, and JavaScript. MGdb is a different shape and size nanoparticles. Decreases in
website which contains the information of predicted size and spherical shape strongly affect the adsorption
genes of the whole genome and the unigenes annotated and activation of the reactants. An attempt has been
by homologous genes in other species, GO (Gene made to obtain unique balance of physical parameters
Ontology) terms which provide a quick look of the traits to set a strategy for selection of specific structures of
in which they are involved. This web based portal gold nanoparticles for enhancing catalytic properties
belonging to different mango cultivars could be a in degradation of organic pollutants.
millstone in the assessment of the research, Key words: Gold nanoparticles, Green technology,
development of the medicines, understanding genetics Trichoderma.
and provides useful bioinformatics solution for analysis
of nucleotide sequence data. We report here world’s SIII/P-13
first web-based genomic resource particularly of mango
for analysis and comparative studies of different mango Histopathological Studies of Drought
cultivars. Tolerant and Sensitive Rice Varieties
Key words: RNA-seq, Annotation, Gene Ontology, in Presence of Endophytes under
Drought Condition
Touseef Fatima*, Isha Verma, Sanjeev Kumar and
Synthesis of Bioengineered Gold Poonam C. Singh
Nanoparticles for Enhancing Catalytic CSIR-National Botanical Research Institute, Lucknow-
226001, INDIA, Email: taus.fatima@gmail.com
There exists a large variability in root system
Ashutosh Tripathi*, Shipra Pandey, Ved Prakash architecture in cultivated rice varieties which
Giri, Vivek Pandey and Aradhana Mishra consequently determines its drought tolerance ability.
Division of Plant Microbe Interactions, CSIR-National Roots are sensitive to abiotic and biotic stresses and
Botanical Research Institute, Rana Pratap Marg, Lucknow- respond at all levels of plants’ structure and function
226001, INDIA, Email: mishramyco@yahoo.com including morphological and anatomical traits. The
drought tolerance or avoidance in rice is largely
Biosynthesis of nanoparticles is an emerging
considered to be dependent on the deep rooting seminal
technology that is green, cost-effective and eco-friendly
and primary roots and are the targets for breeding
but the optimization for its maximum activity is doubtful
varieties better adapted to drought. To understand the
thus there is a need of improvisation for maximum
morphological plasticity, which affect the water
catalytic activity. The main aim of our work is to
harvesting property we studied the morphological and
synthesize gold nanoparticles by cell free extract of
anatomical differences induced by PEG6000 in two
Trichoderma viride, a reducing and stabilizing agent
contrasting rice varieties, Heena (drought resistant) and
with effective potency and improved catalytic

Abstracts 27-30 November, 2018, NBRI, Lucknow 93

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Kiran (drought sensitive), in both absence and presence mg kg-1 dw) and serine (17.53 mg kg-1 dw) were
of rice endophytes. The Morphological and Anatomical significantly higher in seeds of fusant inoculated
features of Heena and Kiran varieties were studied in chickpea. Mineral contents like i.e. Cu (157.73 mgkg-
presence of 20% PEG6000. The sampling was carried dw), Co (0.06 mg kg-1 dw), Ni (1.85 mg kg-1 dw), Zn
out 1, 3, 5, 7 and 10 days after PEG6000 treatment. (157.73 mg kg-1 dw) and S (16.29 mg kg-1 dw) were
The sensitive variety appears to give a spontaneous significantly improved. Fusant inoculated plants showed
response and immediately starts increasing the root better biocontrol and defence activities in chickpea from
length similar to hydrotropism to explore deeper water 20 to 35% during biotic stress. This is one of the first
while the tolerant one is more planned to first increase report on increase in amino acids and mineral content
the root numbers which are structurally and of chickpea by fusant inoculation.
physiologically adapted to drought and then grow in Key words: Protoplast fusant, Trichoderma, Amino acids,
size to get maximum benefit. Increased prevalence of Nutritional value, Biocontrol.
tight packing of cells in the exodermis and lignified
sclerenchyma layer which improves its effectiveness SIII/P-15
as a barrier and prevent loss of water was observed in
presence of the microbial intervention. Increased Molecular Diversity and
lignification and thickened walls in presence of bacteria Characterization of Indigenous Salt
make large negative pressures possible and help in
water uptake. Tolerant Plant Growth Promoting
Key words: Rice, Drought, Endophyte, Heena, Kiran. Rhizobacteria Isolated from Different
Agro-Climatic Zones of Uttar Pradesh
Sankalp Misra, Shashank Kumar Mishra, Vijay
Modulation in Nutritional Value and Kant Dixit, Swati Gupta and Puneet Singh
Defence Activity of Chickpea by Chauhan*
Supplementation of a Novel Division of Plant Microbe Interactions, CSIR-National
Botanical Research Institute, Lucknow-226001, INDIA,
Trichoderma Fusant Email: puneetnbri@gmail.com

Pratibha Verma*, Nishtha Mishra, Priyanka A extensive study for 9 agro-climatic zones of
Chauhan and Aradhana Mishra Uttar Pradesh, India was conducted for the isolation
and characterization of salt tolerant 1-
CSIR-National Botanical Research Institute, Lucknow-
226001, INDIA, Email: mishramyco@yahoo.com
aminocyclopropane-1-carboxylic acid (ACC)
deaminase possessing plant growth promoting (PGP)
Due to rise in global population and food rhizobacteria for salt stress amelioration in rice. For
consumption, yield of agricultural crops has to be this study, we have isolated 1125 bacteria having the
increased substantially in the coming years. To fulfill ability to tolerate 1M NaCl and screened for utilizing
the dietary requirements diminishing nutritional value ACC as sole nitrogen source. The resultant 77 isolates
of the grains has to be maintained by improving the were further evaluated for seed germination assay, PGP
agricultural practices. Legumes and cereal crops are and abiotic stress tolerance ability in vitro. This
important staple food crops with high nutritional values evaluation revealed 15 potent rhizobacteria representing
in amino acids content, carbohydrate content etc. In each agro-climatic zone and salt stress mitigation in
the present study we have used a protoplast fusant vitro. In particular, the biomass obtained for bacteria
(H. lixii MTCC 5659) for enhancing the nutritional value coated rice seedlings were corroborated with the
and defence activity in chickpea. Essential amino acids; performance of isolates exhibiting maximum IAA
methionine (9.82 mg kg-1 dw), cysteine (2.61 mg kg-1 production. Surprisingly based on 16S rRNA, much of
dw), glycine (11.34 mg kg-1 dw), valine (9.26 mg kg-1 the propitious isolates belonged to same specific epithet
dw), and non-essential amino acids; aspartic acid (39.19 exhibited variedly in their characteristics. Overall,

94 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Bacillus spp. was explored as dominant genera in toto metal contamination sites. ã-Proteobacteria was found
with highest distribution in Western plain zone followed to be the most abundant bacterial community followed
by Central zone. Therefore, this study provides a by Actinobacteria, Firmicutes, â-Proteobacteria and á-
counter-intuitive perspective of selection of native Proteobacteria. Overall, the present study leads to a
microflora for their multifarious PGP and abiotic stress better understanding of the interactions between soil
tolerance abilities based on the agro-climatic zones to variables, soil enzyme activity, functional diversity, and
empower the establishment and development of more complex bacterial communities in As-contaminated
suitable inoculants for their application in agriculture paddy soils, and provides useful information of
under local stress environments. indigenous populations with potential application to
Key words: Molecular Diversity, Salt, Rhizobacteria, Agro- improvement in soil remediation processes and explore
Climatic Zones. the deep insight of microbiological imbalance in soil
SIII/P-16 Key words: Heavy metal, Paddy, Actinobacteria, Firmicutes,
Assessment of Bacterial Community
as Fertility Indicators in Response to SIII/P-17
Heavy Metal Contamination in Paddy Characterization of Potential Microbes
Fields Soil for Arsenic Stress Amelioration in Rice
Nikita Bisht, Shashank Kumar Mishra, Swapnil Vandana Anand, Salil Kumar Arkvanshi*, Sonal
Pandey, Sankalp Misra, Vijaykant Dixit and Puneet Srivastava and Suchi Srivastava
S. Chauhan*
Division of Plant Microbes Interaction, CSIR-National
Division of Plant Microbe Interactions, CSIR-National Botanical Research Institute, Lucknow-226001, INDIA,
Botanical Research Institute, Lucknow-226001, INDIA, Email: saliltheredeemer@gmail.com, s.srivastava@nbri.res.in
Email: puneetnbri@gmail.com
Arsenic (As), a ubiquitous metalloid frequently
Heavy metal contamination especially arsenic is present in most environmental samples like soil, water,
a huge problem worldwide. Environmental issues etc. The contamination of this toxic element has become
related to heavy metal contamination draw a great a menace for the human health and natural ecosystems
concern about the soil quality of crop cultivating lands. worldwide. Millions of people are affected by the “As”
Therefore, it is essential to know the status of soil quality poisoning. Rice is popular for more efficient As
or fertility of the areas having high amount of heavy accumulation as compared to other crops due to its
metal, especially As. It is also necessary to evaluate high water requirement. Presence of As mainly in the
the impact of microbial community structure in those form of arsenite As(III) in anaerobic rice field
areas. The present work was done to examine the soil conditions and its transportation via over-expressed
physico-chemical properties, functional, and silicic acid transporters has added to the adversity.
uncultivable microbial diversity of As contaminated Microbes are known to play significant role in
paddy field soil from West Bengal, India. The result detoxification of this metalloid. Microbial activity is
revealed that the fertility indicators and growth-limiting linked to the biogeochemistry of arsenic and is involved
factors were negatively correlated with the heavy metal in its biotransformation. Several bacteria, archaea, and
stress except the soil total organic content which fungi have developed different detoxification
demonstrated significant positive correlation with the mechanisms, which include the reduction of arsenate
heavy metals. In case of functional diversity of soil, all to arsenite; oxidation of arsenite to the arsenate;
the considered diversity indices exhibited no specific immobilization, complexation, vacuolar sequestration
pattern along with the availability of heavy metals. and its methylation to less toxic organic form. The
Moreover, we observed a very complex and indifferent present study deals with the identification,
pattern of bacterial community composition in the heavy characterization and elucidation of detoxification

Abstracts 27-30 November, 2018, NBRI, Lucknow 95

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

mechanisms of different bacterial isolates from different production of siderophore, redox system on cell
parts of India. In addition to this, the strains were also membrane, chelated ligands and acidification of nearby
characterized for different plant growth promoting traits soil by organic acid synthesis. The plant growth
viz. indole-3-acetic acid and siderophore production, promotion activity is beneficial to this approach. Thus,
and phosphate solubilization. Three strains from very small increases in bioavailable MN in edible crop
Bulandshahr, U.P., India NBRIB4.14 (Bacillus mainly staple food implement effective impact on human
tequilensis), NBRIB5.12 (Bacillus sp.) and health.
NBRIB5.12 (Lysinibacillus xylanilyticus) with Key words: Malnutrition, Micronutrient, Biofortification.
arsenate reductase, methyl transferase and arsenite
oxidase activity has been selected as best “As” tolerant SIII/P-19
strains. Assessment of arsenic stress amelioration using
rice as a host plant under greenhouse condition is under Isolation, Production and Reaction
Conditions Optimization of Amidase of
Key words: Arsenic, Abiotic stress, Methyl transferase,
Arsenite oxidase, Arsenate reductase.
Bacillus sp. MNB-1

SIII/P-18 Monica Sharma1* and Monika Geetanjali2

1Department of Biotechnology, School of Life Sciences,
Alleviation of Micronutrient Babasaheb Bhimrao Ambedkar University, Vidya Vihar,
Lucknow-226025, Uttar Pradesh, INDIA; 2Department of
Malnutrition through Biofortification Biotechnology, Delhi Technological University, Main
Bawana Road, Delhi-110048, INDIA, Email:
Kriti 1,2 *, Shekhar Mallick 2 , Suchi Srivastava 2 , monashimla@gmail.com
Vandana Anand1 and Kumkum Mishra1
Amidase (EC, a nitrile metabolizing
Department of Botany, Lucknow University, Lucknow-
enzyme, belongs to the family of hydrolases, which acts
226007, INDIA; 2Plant Ecology & Climate Change Division,
CSIR-National Botanical Research Institute, Lucknow-
on carbon-nitrogen bonds other than peptide bonds. It
226001, INDIA, Email: kriti.v02@gmail.com is an inducible intracellular enzyme, which is involved
in nitrogen metabolism. It catalyzes hydrolysis of amide
In developing country like India most of the to carboxylic acid and ammonium. Amidases find wide
population depends upon staple food (cereal grains like, applications in bioremediation, various industries as
rice, wheat, maize, barely) to fulfill their daily energy commodity chemicals (e.g. acrylic acid, nicotinic acid
requirement. The majority of people who live in poverty, etc.) or pharmaceutically important acids (organic acids
suffer from MN deficiency (hidden hunger as well as and hydroxamic acids). Here we report the isolation,
malnutrition). The main reason for MN deficiency is production and reaction condition optimization of
due to dependency upon monotonous diet that contain mesophilic amidase was undertaken. An aliphatic
of cereal grains despite having enough MN (Fe, Zn selective amide degrading gram positive Bacillus sp.
and Mn). As known when these foods grown in MN MNB1 strain was isolated from thermal spring soil of
deficient soil, there will be automatically decrease in Manikaran, Himachal Pradesh, India. Amidase
grain. Anemia (Fe deficiency), growth retardant, production and reaction conditions were optimized.
immune dysfunction, cognitive impairment (Zn Amidase of Bacillus sp. MNB1 exhibited maximum
deficiency) and Asthma, severe birth defects (Mn activity in M2 medium at temperature 37oC, pH 7.0 in
deficiency) are some example of malnutrition. the presence of acetamide as inducer. The enzyme
Biofortification approaches as agronomic, breeding, showed maximum activity at pH 7.0, temperature 37oC
generating cultivars that effectively solublize, uptake and 40-50 mM of acetamide as substrate.
and translocate nutrient to edible part. Biofortification
through microbes could be inexpensive, sustainable and Key words: Amidase, Hydroxamic acids, Nitrile metabolizing
enzyme, Bacillus sp.
eco-friendly practice. Microorganisms those have
ability to solublize insoluble form of minerals with

96 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)


Influence of Heavy Metal on the Soil Development of an Algal Consortium

Microbial Community Structure and for Arsenic Removal from Water
Root Exudates
Anurakti Shukla and Sudhakar Srivastava*
1,2 1
Naina Marwa *, Meenakshi Khushwaha , Gauri Institute of Environment and Sustainable Development,
Saxena2 and Nandita Singh1 Banaras Hindu University, Varanasi-221005, INDIA, Email:
sudhakar.iesd@bhu.ac.in, sudhakar.srivastava @gmail.com
Plant Ecology and Environmental Sciences, CSIR- National
Botanical Research Institute, Rana Pratap Marg, Lucknow- Arsenic (As) presence in groundwater at higher
226001, INDIA; 2Department of Botany, University of than recommended levels is a serious problem in India
Lucknow, Lucknow-226007, INDIA, Email: naina.marwa@ and Bangladesh. The presence of As has been reported
gmail.com mainly in Gangetic plains in the states of West Bengal,
The aim of the study was to observe the soil- Bihar, and Uttar Pradesh. It causes serious health
microbial and root exudates cross-talk under the concerns ranging from skin disorder to cancer.
influence of Arsenic, a heavy metal. To detect the effect Therefore, As remediation in a cost-effective, practical
of two Ashyper-tolerant, plant growth promoting and feasible manner is necessary. In this study, different
rhizobacteria (PGPR) strains isolated from the As algal strains (green algae, blue green algae, diatoms)
polluted site of West Bengal, India, on the rhizospheric were collected from water bodies in Varanasi as well
microbial community, their respective richness, diversity as As affected regions of Kolkata and Nadia, West
was assessed. Shannon, McIntosh and Simpson Bengal. The arsenic tolerance behaviour of different
diversity and their related evenness were estimated algal strains and diatom species (Gloeotrichia,
for microbial substrate utilization pattern on Biolog MT Oscillatoria, Phormedium, Navicula, Gyrosigma,
plates. The first two components, PC1 and PC2 Nitzschia, etc.) was assessed and selected algal strains
cumulatively accounted for 75.81% variance in 120 hr of Phormedium and Oscillatoria were used for
of average well colour development (AWCD) consortia formation. The growth of algae in consortium
normalized data. Individually PC1 showed 40% was standardized with respect to pH, As concentration
variance whereas 35.7% variance by PC2. The PCA and algal biomass. The consortia in optimal conditions
plot on the basis of carbon source utilization pattern was used for As removal from BG-11 medium
displayed significant changes in microbial dynamics in containing 200 µM, 400 µM, 600 µM arsenite [As(III)]
inoculated samples. Furthermore, the root exudates for 15 d and samples were analyzed for As content.
profiling was done in the rhizospheric soil through GC- The consortium was found to show significant As
MS to investigate the role of heavy metal in its removal potential.
regulation. The detection of compounds like glycine, Key words: Algae, Arsenic, Oscillatoria, Phormedium,
sucrose, d-glucosamine, etc. in bacteria inoculated Removal.
samples reveal the constructive contribution of the
strains in maintaining the rhizospheric environment SIII/P-22
conducive for plant growth. Our findings reveal that
the heavy metal influences the microbial community Use of Bioinoculants to Augment
thus adversely affects the root exudates secretion Growth and Yield of Soybean (Glycine
which is essential for maintaining plant-microbe
interaction and root expansion. Loss of microbial
max) under Drought Stress Condition
diversity due to heavy metal can be restored through
Anamika Dubey and Ashwani Kumar*
inoculation of efficient microbes. The maintenance of
rhizospheric microbial balance is important for better Metagenomics & Secretomics Research laboratory,
remediation results. Department of Botany, Dr. Harisingh Gour Central University,
Sagar-470003, MP, INDIA, Email: ashwaniiitd@hotmail.com
Key words: Heavy metal, Arsenic, Microbial diversity, Plant
growth promoting rhizobacteria.

Abstracts 27-30 November, 2018, NBRI, Lucknow 97

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Soybean is the important crop of Madhya production of ethylene leads to abnormal root growth,
Pradesh and largely harvested for production of this leads to impart a visible dent on plant growth and
vegetable oil. There are different types of abiotic and development. ACC synthesized in root is transported
biotic stress faced by the plants from seedling to to plant shoot where it is converted to ethylene by ACC
production stage. Drought stress adversely affects the oxidase. Bacteria being most abundant microorganisms
plant growth and yield of soybean. The present study in the rhizosphere influence the plants’ physiology to a
investigated the effect of inoculation of plant-growth greater extent, especially considering their
promoting rhizobacteria (PGPR) strains AKAD 1-2 competitiveness in root colonization, hence, referred
(Pantoea agglomerans), AKAD 1-3, (Bacillus as plant growth-promoting rhizobacteria (PGPR).
subtilis), and AKAD3-5 (Micrococcus luteus) in Several studies revealed increased health and
improving drought tolerance in soybean. These PGPR productivity of various plant species under both normal
strains were screened for different PGPR activities and stressed conditions by the application of plant
like PSB, IAA production, ammonia production, growth promoting rhizobacteria. PGPR containing ACC
catalase, chitinase, and HCN production. Effect of deaminase can lower the impact of various
PGPR inoculation on various morphological, environmental stresses such as flooding, heavy metals,
physiological, biochemical parameters was studied soil-borne phytopathogens, drought and high salt on host
under drought stress. Root colonization at the surface plant. ACC deaminase can hydrolyse the ACC,
and interiors of roots were shown using scanning intermediate precursor of ethylene to á-ketoglutaric
electron microscopy (SEM). Drought stress acid and ammonia thus inhibiting the transport to shoot
significantly affected various growth parameters, and thus retards the harmful effect of ethylene on plant.
integrity of the membrane, water status, accumulation Inoculation of ACC deaminase-containing PGPR in
of the osmolyte, which were positively ameliorated by association with plants subjected to a wide range of
PGPR-inoculation in soybean. The PGPR strain abiotic stresses results in enhanced plant tolerance
AKAD 1-2 was found to be the best in terms of against exposed stressors.
influencing biochemical and physiological status of the Key words: Plant Growth Promoting Rhizobacteria (PGPR),
seedlings under drought stress. Our report demonstrate ACC deaminase, Rhizobacteria.
the role of PGPRs (Pantoea agglomerans, Bacillus
subtilis, and Micrococcus luteus) in plant growth SIII/P-24
promotion of soybean under drought stress. The study
reports the potential of PGPR in alleviating drought Biotechnological Investigation for
stress in soybean. Propagation and Conservation of
Keyword: Soybean, Bioinoculants, PGPR, Drought stress, Beles (Opuntia ficus-indica (L.) Mill
in Tigrey Region of Ethiopia (Africa)
Shastri Prasad Shukla 1 *, Zinabu Hailu 2 and
Applications and Mechanism of Plant Yemene Kehsay3
Growth Promoting Rhizobacteria 1
Department of Horticulture, College of Agriculture &
(PGPR) Containing ACC Deaminase Environmental Sciences (Beles Institute), Adigrat University,
Adigrat, ETHIOPIA, AFRICA; 2Research and Community
under Water logging Conditions Service, Beles Institute, Adigrat University, Adigrat,
ETHIOPIA, AFRICA; 3Beles Institute, Adigrat University,
Jyoti Prakash Adigrat, ETHIOPIA, AFRICA, Email: sp_shukla2002@
yahoo.co.in, zinisheh@gmail.com, yemane02@gmail.com
Amity Institute of Biotechnology, Amity University, Uttar
Pradesh, Lucknow Campus, Lucknow-226028, INDIA, Email: Cactus having about 130 genera and 1,500 species
jprakash@lko.amity.edu of cactaceae family. Of these, Opuntia ficus-indica
Ethylene production in plant roots is accelerated (L.) Mill. commonly known as prickly pear or Beles is
in response to both biotic and abiotic processes. Over a xerophytic, succulent, CAM (Crassulacean Acid

98 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Metabolism) plant introduced in Ethiopia (Africa), Microbial Technology Division, CSIR-Central Institute of
particularly to Tigray region between 1848 and 1920. Medicinal and Aromatic Plants, Lucknow-226015, INDIA;
Throughout the African continents and especially in Department of Botany, University of Lucknow-226007,
Ethiopia, prickly-pear cultivation has long had an INDIA, Email: biosuman14@gmail.com
important role both in subsistence agriculture and in Growing demand for mint oil in food and flavours
the intensive fruit production. Beles is a multipurpose requires eco-friendly agricultural practices. Plant
plant with great potential as source of livestock feed in growth promoting rhizobacteria or fungi alone or in
Tigray region and Ethiopia at large. It is known as a combination become useful soil microorganisms
multipurpose plant since it can be used for human food improving production and control abiotic and biotic
(fruits and vegetables), medicinal and ornamental plants, stresses. The present study was conducted to assess
animal forage, natural wind break barrier, soil stabilizer, the potential of co-inoculation of Trichoderma spp. with
re-vegetation resource to control water and wind erosion the plant growth promoting rhizobacteria (PGPR) on
in eroded soils. The sweet fruit of this plant is known the growth and essential oil yield of Mentha arvensis
as “Tuna” useful as human food and entire plant can cv. “Kosi” under pot as well as field conditions. The
be cultured as raw-industrial material to produce several results revealed that dual inoculation of Trichoderma
sub-products such as jam, wine, candies and jellies etc. spp. and Brevibacterium spp. increased plant growth,
It is also source of natural-dye, carminic acid which is oil content, leaf-stem ratio, photosynthetic pigments,
used for colouring fabrics, food, and cosmetics. Stem and nutrient uptake resulting in higher oil yields. The
of this plant has been reported to treat diabetes and density (colony forming unit) of Trichoderma, as well
useful in the cure of hyperlipidemy (excess of lipids in as rhizobacteria, was significantly higher when
the blood) and obesity. O. ficus-indica is well compared to the single inoculations and control.
recognized for their wound healing properties and Scanning electron microscopy revealed significant
anticancer effects. Conventionally, O. ficus-indica is colonization of microbes on the root surface particularly
propagated by seeds but physiological limitations of the for the best combination of Trichoderma and
seeds, such as; low germination rate, genetic Brevibacterium spp. Similarly, under field conditions
segregation, less guarantee of genetic stability, a long the synergistic association of Brevibacterium spp. and
juvenile stage, less availability and low viability, and Trichoderma showed increased plant growth which
slow seedling growth rate are major constraints in the provides evidence that both enhance the growth
production of the population of this species in the nature. promoting abilities of each other significantly improving
Therefore crop improvement programme, microcloning growth and yield of the crop. A higher number of
and ex-situ conservation of the species is underway propagules, both of Brevibacterium spp. and
which is a principal objective of the Beles Institute of Trichoderma may exert similar crop growth benefits
Adigrat University, Adigrat, Ethiopia (Africa). in the subsequent crops while higher the population of
Key words: Opuntia ficus-indica, Biodiversity, Trichoderma may protect the subsequent crops from
Conservation, Micropropagation, CAM plant. many phytopathogens.
Key words: Mentha arvensis, Trichoderma spp., Plant
SIII/P-25 growth promoting rhizobacteria, Brevibacterium spp.,
Scanning Electron Microscopy.
Evaluation of Selected Compatible
Microbial Consortia for the Growth SIII/P-26
Promotion and Increased Essential Oil Algal Biomass Pretreatment for
Content in Mentha arvensis under Enhanced Methane Potential
Field Conditions
Shashi Bhushan1*, Mohit Singh Rana2 , Mamta1
Suman Singh 1 *, Arpita Tripathi 1 , Deepamala and Sanjeev Kumar Prajapati1
Maji1, Anju Yadav1, Poornima Vajpayee2 and Alok 1
Bio-Resource Engineering Lab (BREL), Department of
Kalra1 Chemical and Biochemical Engineering, Indian Institute of

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Technology (IIT) Patna, Bihta, Patna-801106, Bihar, INDIA; Abiotic stresses are one of the major barriers for
Department of Energy and Environment, National Institute crop productivity. The native rhizospheric soil
of Technology (NIT) Trichy, Tiruchirappalli-620015, Tamil microflora has limitations to withstand against these
Nadu, INDIA, Email: shashiascientist@gmail.com abiotic stresses and is mostly exclusive to plant growth
Human kind receives their major portion of energy promotion. In the current study, an attempt was made
needs from fossil fuels. These fuels are non-renewable to evaluate soil bacteria from extreme environments
and their reservoir is finite. Ever increase energy of volcano soils based on plant growth promoting and
demands can be addressed through biofuels. Among abiotic stress mitigating characteristics. The screening
the available biofuel substrate, algal biomass shows led to 08 (NBRISH4, NBRISH6, NBRISH10,
most promising feed stock for biodiesel, biomethane, NBRISH11, NBRISH13, NBRISH14, NBRISH16 and
and bioethanol. Microalgae contain chlorophyll and NBRISH26) bacterial isolates capable of withstanding
perform photosynthesis at higher rate as compared to stress namely temperature (up to 45° C), salt (up to
the terrestrial plant. They can trap atmospheric carbon 2M NaCl) and drought (up to 60% PEG) in vitro.
dioxide and fixed them in biomass. Their cell wall is Further, the selected isolates were outstanding in vitro
composed of cellulose, which make them tuff and temporal performance with regards to their survival
resistant to many cell disruption techniques. To enhance (in terms of colony count), auxin production, phosphate
the biofuel potential of the algal biomass, it is advisable solubilization, biofilm formation, alginate and exo-
to perform pretreatment steps. These pretreatment polysaccharide production abilities under abiotic stress
steps enhance the anaerobic digestibility of cell wall (40°C temperature; 500 mMNaCl representing salt;
and release the high value cellular content. This paper 250 mM LiCl representing drought) conditions. In vivo
gives a brief idea about the available pretreatment treatments of each bacterium to maize plants resulted
modules. It also present the results of physical into significant enhancement in root and shoot length,
pretreatment (microwave, ultrasonic and thermal) root and shoot fresh and dry weight and no. of leaves
performed on Chlorella pyrenoidosa. Among tested per plant (NLPP). Overall, the plant growth promoting
technique microwave seems most efficient in terms of and abiotic stress tolerance ability was prominent for
soluble COD (Chemical oxygen demand) recovery. The NBRISH6 bacterial isolate which was identified as
released COD enhance the methane potential of algal Ochromobactrum sp. using 16S rRNA based
feedstock. phylogenetic analysis. Therefore, NBRISH6 having
multiple plant beneficial and abiotic stress tolerance
Key words: Microalgae, Biofuel, Pretreatment, COD,
Methane. characteristics shall be considered for its application in
agriculture under stress environments.
SIII/P-27 Key words: Rhizobacteria, Ochromobactrum, Abiotic
Characterization of Plant Growth
Promoting Rhizobacteria Isolated SIII/P-28

from Extreme Environment (Volcanic Somatic Embryogenesis Responsive

Soil) with Multiple Plant Beneficial Kinase (OsSERK) and Myb Related
Traits along with Abiotic Stress Gene (OsGLK) Improved Somatic
Tolerance Capabilities Embryogenesis and Regeneration
Shashank Kumar Mishra, Swapnil Pandey,
Potential in Rice (Oryzasativa L.)
Sankalp Misra, Vijay Kant Dixit, Nikita Bisht and
Yuvraj Indoliya1,2*, Poonam Tiwari1 and Debasis
Puneet Singh Chauhan*
Division of Plant Microbe Interactions, CSIR-National 1
Botanical Research Institute, Lucknow-226001, INDIA, CSIR-National Botanical Research Institute (CSIR-NBRI),
Email: puneetnbri@gmail.com Rana Pratap Marg, Lucknow-226001, INDIA; 2Academy of

100 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Scientific and Innovative Research (AcSIR), Anusandhan silencing and overexpression analysis among japonica
Bhawan, 2 Rafi Marg, New Delhi-110001, INDIA, Email: and indica rice subspecies. Further phenotypic analysis
yuvi_indolia@yahoo.com of OsSERK and OsGLK overexpressing lines of PB-1
Rice (Oryza sativa L.), a major cereal crop for variety (Indica subspecies) showed significant
around half of the world population, also acts as a model increase in somatic embryogenesis and regeneration
crop for monocot plants in order to study various potential compared to wild type. On the other hand,
developmental, environmental and biochemical aspects knock down lines of Nipponbare variety (Japonica
due to its small size genome (430 Mb), better genomic subspecies) showed notable decrease in somatic
identity with other cereal crops and comparatively better embryogenesis and regeneration efficiency compared
transformation system using agrobacterium mediated to wild type. Functional significance of both the genes,
transformation. Compare to japonica, indica rice has in terms of in vitro plant regeneration may be used as
been less responsive to in vitro culture. In the present an efficient tool for improving agrobacterium mediated
study, comparative somatic embryogenesis and transformation, especially in cereal plants.
regeneration efficiency of two genes (OsSERK and Key words: Rice, Somatic embyrogenesis, Japonica, Indica.
OsGLK) was carried out using amiRNA mediated gene

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

102 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Session IV
Environmental Impact Assessment

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

104 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SIV/L-1 time, it will introduce conference audience to future

international collaborations aimed at detailed
Coal-Related Environmental Pollution examinations of pollutant cycles in the environment,
has been Affecting Our Lives and applications of clean-up bioremediation strategies.
Key words: Coal mining, Toxic trace metals, Organic
Gordana Meduniæ pollutants, Human health risk.
University of Zagreb, Faculty of Science, Department of
Geology, Horvatovac 95, 10000 Zagreb, CROATIA, Email:
gmedunic@geol.pmf.hr, gordana.medunic@gfz.hr
Some Insights into Strategies for Size
Since 2013, my multidisciplinary environmental
research has been focused on highly sulfurous (up to
Fractionated Phytomonitoring of
4-8% total S) soil polluted by Croatian super high- Airborne Particulate Matter (PM) and
organic-sulfur (SHOS) Raša coal (up to 11-14% total Associated Metals
S). Due to coal mining, utilization, and combustion
activities, large amounts of S, potentially toxic trace Sudhir Kumar Pandey
metals (Cd, Cr, Cu, Pb, U, V, Zn, etc.), metalloids (Se),
Department of Botany, School of Studies in Life Sciences,
and organic pollutants (polycyclic aromatic
Guru Ghasidas Vishwavidyalaya (A central University),
hydrocarbons, PAHs) have been released into acid-
Bilaspur-495009, C.G., INDIA, Email: skpbhu@gmail.com
vulnerable, coastal karst environment (Istrian Peninsula,
North Adriatic, Croatia). The area is urban, densely The presentation discusses about the advances
populated touristic region. The results are published in in phytomonitoring strategies for PM and associated
high IF WoS Core Collection journals (Science of the toxic metals in strong source environments (e.g., urban
Total Environment, Chemosphere, Environmental Earth roadside environments). To this end, the plant species
Sciences and International Journal of Coal Geology). naturally growing in the affected areas were chosen
The objectives of the studies were to determine the and PM samples were collected in different
distributions of the mentioned pollutants and rare earth environmental matrices. For quantification of PM in
elements (REEs) in soil, ash and SHOS coal. I have, different size fractions, plant leaves were chosen, and
together with my colleagues from various scientific an effective quantification method was developed
disciplines (biology, chemistry, physics, biochemistry, through scanning electron microscopy-based imaging
metallurgy, etc.), addressed the problem by applying and image-based counting method. For monitoring of
several analytical approaches to determine toxicity PM bound toxic metals, the conventional atomic
potential of polluted soil for life forms (animals and absorption method was employed in combination with
plants). We have tested soil samples by cytotoxicity, SEM-electron dispersive spectroscopy (EDS). The
genotoxicity, and phytotoxicity methods. We have also SEM-EDS was effective enough to screen the
calculated a risk for human health posed by several maximum number of toxic metals present in PM of
routes (dermal contact as well as inhalation and urban air. The strategies adopted in these studies have
ingestion). As the results were indicative of statistically two-fold implications: (1) rapid phytomonitoring of PM
significant toxicity potential, our work has warranted and PM bound toxic metals in air and (2) comparative
further research on this environmental topic. Therefore, evaluation of plant species for their suitability in
latest/ongoing research is involving collaboration with phytoremediation of PM and PM bound metals. The
leading US, Chinese, Indian, Italian, Spanish, and study further highlights the status and prospects of size
Slovenian coal geochemistry, clean-coal technology, fractionated phytomonitoring of PM and PM bound
chemistry, physics and environmental scientists. The metals in air.
talk will summarize most important findings of this Key words: Toxic metals, SEM-EDS, Urban roadside, Image
comprehensive multidisciplinary research. At the same based quantification, Phytomonitoring.

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SIV/O-1 adoption by farmers. Seeing is believing strategy can

be used to change the mindset of farmers in different
Impact Assessment of Conservation regions with locally modified CA package of practices.
Agriculture Systems on Environment, CA insights a way forward scenario of agriculture in
Indian IGP that promises to increase the farmer’s
Rural Community and Economy of income and diversify the agriculture with its
Farmers in Indo-Gangetic Plains of multifaceted ecosystem services.
India Key words: Conservation agriculture, Constraints in CA,
Prospects of CA, Farmer’s Perspective, IGP zero tillage.
Anjali 1 *, Ajay Kumar Mishra 2 and V. Venkat
Ramanan1 SIV/O-2
School of Inter-Disciplinary and Trans-Disciplinary Studies, Prioritizing Invasive Aquatic
Indira Gandhi National Open University, New Delhi, INDIA;
Terrestrial Ecosystem Management Laboratory, Graduate Macrophytes using a Comprehensive
School of Global Environmental Studies, Kyoto University, Risk Assessment (CRA) Approach for
Kyoto, JAPAN, Email: anjali0666@gmail.com
their Control and Management in
India is an agrarian economy and conservation
agriculture (CA) is one of the emerging practices that Tropical Floodplains
play a pivotal role in ensuring productivity, improving
soil health and mitigating climate change. The principal Tapati Das 1 *, Nami Prasad 1 and Dibyendu
CA technologies involve minimum soil disturbance, Adhikari 2
permanent soil cover through crop residues or cover 1
Department of Ecology and Environmental Science, Assam
crops, and crop rotations that offer multiple benefits in University, Silchar-788011, INDIA; 2Department of Botany,
resource conservation. Implementation of CA in Indo- North-Eastern Hill University, Shillong-793022, INDIA, Email:
Gangetic plains (IGP) not only offset straw burning das.tapati@gmail.com, namip1386@gmail.com,
but also contributes towards sustainable agriculture. dibyenduadhikari@gmail.com
The technologies of CA provide opportunities to reduce The tropical floodplain located in the Barak river
the cost of production, save water and nutrients, increase basin of Assam in northeast India, a part of the Indo-
yields, increase crop diversification, improve efficient Burma biodiversity hotspot, is dominated by varied
use of resources, and benefit the environment. Efforts aquatic systems. These systems play a vital role in
for nearly two decades have been made in India to sustenance of life of the local communities, particularly
develop, refine and disseminate CA technologies to the poor, through various aquatic resources. However,
different agro-ecology of IGP. As a result, significant most of these systems are infested by aquatic
progress has been made since then but still, there are macrophytes (AMs) some of which have been reported
several constraints like conventional mindset of as invasive in the Global invasive species database of
farmers, lack of reach of viable technologies to farmers the IUCN Species Survival Commission and
of eastern IGP compared to western IGP etc. that affect Environmental Information Systems (ENVIS) database
the adoption of CA. Present agriculture urges a of Ministry of Environment, Forest and Climate Change,
paradigm shift from extensive to intensive agriculture Government of India. As invasive species often
in the IGP. Therefore, this study was conducted with profoundly affect the environment, economy, human
the objectives 1) to quantify the impact of CA on socio- health and the overall flow of various ecosystem
economic constraints and prospects in the IGP of India, services it is important to assess the invasiveness
2) to assess the key factors governing the adoption of potential of these species for prioritizing them under
CA on regional and local scale, 3) to develop a linkage various levels of threat for their suitable management
between all working groups that influences CA adoption options. In the present study we categorized the
4) to propose remedy measures and alternatives based dominant AMs in tropical floodplain located in Barak
on local modifications that would enhance the CA river basin of Assam under different risk classes using

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the CRA approach. This involved scoring of five major basis. Water quality of the Ghat has been assessed on
attributes/aspects of species invasion through direct a regular basis during the summer, winter and rainy
measurements in the field, deductions from field season, in the course of the present study. Parameters
observations, and inferences based on information like turbidity, temperature, pH and EC were measures
available in the literature. The attributes were (i) species on spot by thermometric and potentiometric method,
morphological features, (ii) biological characteristics & respectively. Water samples were collected in BOD
dispersal ability, (iii) distribution potential, (iv)ecological bottles as well as pearl pet bottles for investigating
impact, and (v) difficulty to control. The study revealed various parameters including Alkalinity, Hardness,
that as more risk of invasion has been observed for Chlorides, Nitrate, total dissolved solids, BOD (5-day),
Eichhornia crassipes, Ipomoea carnea, Phosphate etc. National Environmental Engineering
Alternanthera philoxeroides, Ipomoea aquatica, Research Institute and the American Public Health
Lemna minor, Pistia stratiotes, and Trapa natans, Association protocols were followed for investigating
these species should be prioritized for suitable remedial various physic-chemical parameters. Significant
measures to control their further spread in the study seasonal variation has been observed in the physic-
area. The present study therefore showed that through chemical characteristics of the study site. These
CRA approach it is possible to identify the AMs with variations were also very obvious in accordance with
greater invasion potential which in turn would help the the Hindu festive calendar. An increase in values of
scientists and policy makers in prioritizing such species EC, pH, TDS, turbidity, alkalinity, chloride, BOD,
for their control and management in a suitable way. phosphate etc. have been recorded particularly during
Key words: Invasion potential, Scoring technique, Aquatic the winter months and after Kartik and Maagh months
macrophytes, Tropical floodplains, Barak basin. of the Hindu calendar. Same is true for the period
following the immersion of idols after the two Navratras
SIV/O-3 and Ganesh festival.
Key words: Jaipur Ghat, Physico-chemical analysis, Pollution,
Effect of Religious Rituals on the Pushkar Sarovar, Religious Rites, Seasonal variations.
Water Quality of Jaipur Ghat of
Pushkar Sarovar (Rajasthan)
Assessment of Soil Pollution using
Anita Sharma
Multivariate Techniques and Indexing
Samrat Prithviraj Chouhan Government College, Ajmer,
Rajasthan, INDIA, Email: anitasharma9339@gmail.com Approach
The Pushkar Sarovar, situated in the lap of the Vinod Kumar
Central Aravalli Ranges in the State of Rajasthan, is
perhaps one of the most revered holy lake. It is Department of Botany, DAV University, Jalandhar-144012,
INDIA, Email: vinodverma507@gmail.com
supposed to have originated at the point where Lord
Brahma’s lotus struck the land. Thousands of devotees Soil pollution is one of the most important issues
visit the Sarovar to take holy bath and worship it. Over throughout the globe and increasing urbanization,
the years, this resulted in enhancement of pollution level industrialization and application of pesticides, fertilizers
in the water body accompanied with accelerated etc. on agricultural activities are responsible for soil
eutrophication. Steps have been taken by the local pollution. The present work was designed to study the
administration and various local groups to combat the soil properties (pH, conductivity, soil organic matter,
problem. One of the common decision taken was not phosphorus, calcium and magnesium) and heavy metals
to immerse idols, flowers and ashes into the Lake. A (Cr, Cu, Pb and Co) in soils of Jalandhar District. Cluster
part of the lake has been delimited at the Jaipur Ghat analysis (CA) and principal component analysis (PCA)
for the purpose. The concrete tank built for the purpose showed that anthropogenic activities have great
is cleaned of the debris, thus preventing the Lake from influence on the soil properties and heavy metal content
receiving the pollutants directly and that too on a regular in the soil. The various indices such as contamination

Abstracts 27-30 November, 2018, NBRI, Lucknow 107

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

factor, geoaccumulation index, potential contamination this species under both current and future climate
index, enrichment factor (%), pollution index and change scenarios. Also, the Himalayas is predicted to
potential ecological index showed that soils are be at moderate invasion risk under climate change. The
moderately polluted by the heavy metals. This is the research findings of the present study have practical
first such baseline study in the area and further research applications in predicting invasion hotspots and
needed in order to monitor the soil pollution status of developing region-specific management strategies in
Jalandhar, District. the country.
Key words: Heavy metals, Soil pollution, Jalandhar. Key words: Biogeographic zones, Climate change,
Distributional modelling, Habitat suitability, Invasion.
Potential Distribution and Invasion
Risk Assessment of Parthenium ICT Promotes Environmental
hysterophorus in India under Climate Awareness through ENVIS activities
Change under a National Programs
Sunil Tripathi*, Diwakar Saini, Vineeta Yadav,
Rameez Ahmad 1 *, Anzar A. Khuroo 1 , Maroof
Amrita Awasthi and Pankaj Kumar Srivastava
Hamid1, Bipin Charles2 and Zubair A. Rather1
1 Environmental Technologies, CSIR-National Botanical
Centre for Biodiversity and Taxonomy, Department of
Research Institute, Rana Pratap Marg, Lucknow-226001,
Botany, University of Kashmir, Srinagar-190006, J&K, INDIA;
2 INDIA, Email: sunil9351@gmail.com
Ashoka Trust for Research in Ecology and the Environment
(ATREE), Royal Enclave, Srirampura, Jakkur PO, Bengaluru- Environmental information plays a vital role not
560064, INDIA, Email: rameezkhuroo929@gmail.com only in formulating environmental management policies,
Congress grass (Parthenium hysterophorus L.), but also in the decision making process aiming at
native to Tropical America, is one of the world’s worst environmental protection and improvement of the
weeds with deleterious ecological and economic environment for sustaining a good quality of life of the
impacts. In India, the weed was accidentally introduced living beings. Realizing this need of information system,
from Central America about a century ago and since Ministry set up an Environmental Information System
then it has invaded most of the regions across the (ENVIS) in 1983 to establish a comprehensive network
country. The present study using ensemble modelling in environmental information collection, collation,
approach implemented in biomod2 package has storage, retrieval and dissemination to users. Presently,
mapped the current and future distribution of P. The ENVIS network consists of 66 network partners,
hysterophorus in India and assessed its invasion risk out of which 35 are subject-specific and 31 State/UT.
in different biogeographic zones under climate change These network partners are called ENVIS Centers that
scenarios. The modelling results revealed that about are located in the notable organizations/institutions/State/
65% of the total area in the country is suitable for UT Government Departments/Universities throughout
potential invasion of this species under current climatic the country. ENVIS centre at CSIR-National Botanical
conditions. The model predicted a decrease in overall Research Institute has started in January 2004 and
habitat suitability for this species under future climate focusing on the specific theme of “Plants and Pollution”.
change with more than half of suitable habitat reduced ENVIS Resource Partner-NBRI systematically
under RCP 8.5-2070. Nonetheless, some of the collects, compiles and disseminates data on all the
currently invaded areas will become highly vulnerable diverse aspects of “Plants and Pollution” viz; effects
to invasion under climate change. On downscaling to of different pollutants on plant kingdom and plant
different biogeographic zones, North-east and coastal resources for mitigation ofspecific pollutants. The centre
areas in the country remained as high and moderate is publishing theme based Newsletters (Quarterly),
risk regions respectively to the potential invasion of Subjective News (monthly), thematic bulletins (twice

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

a month), maintaining Databases (34), Case Studies, is resistant or to cope with open cast mining generated
Latest news based on theme, Upcoming Events, dust pollution in and around mining areas as well as
Glossary, Bibliography, technical reports, etc. The beautification of adjacent highways. Maximum
ENVIS RP-NBRI website is designed and fully devoted deposition was recorded in Ficus hispida and followed
on the latest information about the specific theme by Calotropis procera, Butea monosperma, Ficus
“Plants and Pollution” and it helps to the scientists, benghalensis, Allianthus excelsa, Azadirachta
researches, students etc. Those are involved in the indica.
research area of the same. Besides these, ENVIS also Key words: Dust pollution, Chlorophyll content, Ficus spp.,
provides Discussion Forum and a mobile app viz; National highway.
“Green Planner” (Android and itunes app). Further,
ENVIS centre is do various outreach activities to spread SIV/O-8
environmental awareness among the people. The
national and international scientific data present on Determination of Geochemical
ENVIS RP-NBRI website are of immensely use to Properties and Temporal Variation of
several research organizations, town planners, policy
makers, teachers, students including kids and others Ground Water in Kanchipuram
stakeholders to aware about Environmental status District, Tamilnadu, India
especially related to the plants and pollution.
Key words: ENVIS, Environmental Information System, A. Chandrasekaran1* and R. Ravisankar2
Plants and pollution, Pollutants, Green planner. 1
Department of Physics, SSN College of Engineering,
Kalavakkam, Chennai-603110, Tamilnadu, INDIA;
SIV/O-7 2
Department of Physics, Government Arts College,
Tiruvanamalai-606603, Tamilnadu, INDIA, Email:
Open Cast Mining and Impact of Dust chandrasekarana@ssn.edu.in
on Roadside Plant Species at Jhansi- Water is essential for all living organisms for their
Allhabad National Highway existence and metabolic process. The aim of the present
study is to assess the quality and temporal variations
Priyanka Singh and Amit Pal* of ground water in Kanchipuram district, Tamilnadu,
India. In order to assess the water quality, water
Institute of Environment & Development Studies,
samples were collected from 20 different locations in
Bundelkhand University, Jhansi-284128, INDIA, Email:
apu13@rediffmail.com, spriyanka680@gmail.com
pre-monsoon season and geo-chemical properties such
as pH, Total Dissolved Solids (TDS), Electrical
Deposition of dust on roadside plant and its impact conductivity (EC) Chloride (Cl-), Sulfate (SO4 2-),
on leaf i.e. leaf pigment concentration, the carotenoid Calcium (Ca2+), Magnesium (Mg2+), Nitrate (NO3-),
and protein has been investigated on fifteen Sodium (Na +), Potassium (K+), Carbonate (CO3),
selectedroadside plants species namely Allianthus Hydrogen Carbonate (HCO3-) were determined using
excelsa, Azadirachta indica, Butea monosperma, different analytical methods. The measured geo-
Calotropis procera, Cassia fistula, Datura metel, chemical properties are compared with prescribed limits
Ficus benghalensis, Ficus hispida, Ficus infectoria, given by world health organisation (WHO, 2004). The
Ficus religiosa, Holoptela integrifolia, Millettia results of geochemical properties indicates that average
pinnata, Nerium oleander, Phoenix dactylifera and of Ca, Cl, NO3 and pH slightly higher than WHO, 2004
Psidium guajava respectivelyat Jhansi-Allahabad permissible limit whereas other measured properties
National Highway – 76. The variation in terms of dust are lower. Multivariate statistical techniques like
deposition with species specific result observed during Pearson correlation, factor and cluster analysis were
the entire period of study. Declination of leaf pigment applied to water quality variable for evaluation of
concentration, carotenoid content and protein content temporal variations in the study area.
indicate the positive impact of dust pollution. Above
Key words: Ground water, Temporal variations, Multivariate
findings may be helpful to find out some species which
statistical techniques.

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SIV/O-9 irrigational purposes after conventional treatment,

making it unsafe for drinking and irrigation purposes
Comparative Evaluation of however no chemical treatment is needed for the
Hydrogeochemical Processes and groundwater samples analyzed from Doda district as
most part of the Bharderwah Tehsil is free from
Chemometrics of the Groundwater in agricultural, industrial and domestic contamination as
Vijaypur Block of Samba District and reflected in the groundwater chemistry.
Bhaderwah Tehsil of Doda District, Key words: Groundwater, Hydro geochemistry, Carbonate
Weathering, Cation Exchange Capacity, TDS.
Anjali Bala¹*, Pankaj Mehta¹ and Anu Ramotra²
Department of Environmental Sciences, Central University Impact of Conversion of Grassland to
of Jammu, District Samba-181143, J&K, INDIA; 2Government
Higher Secondary School, Kalakote, District Rajouri, J&K,
Agroecosystem on Soil Organic
INDIA Carbon Content in Dry Tropics
Groundwater is one of the most precious natural
Priyanka Soni*, Chandra Mohan Kumar and
freshwater source, which is accessible to human beings.
Nandita Ghoshal
Approximately, 56 % rural Indian access potable water
from tube wells, about 14% from open wells and about Centre of Advanced Study in Botany, Department of Botany,
25% supplied water system depend on groundwater, Institute of Science, Banaras Hindu University, Varanasi-
which is on average 90% of total rural water supply 221005, INDIA, Email: sonipriyanka840@gmail.com,
cmbhu05@gmail.com, nghoshal14@gmail.com
(NSSO 2006). The quality of groundwater is generally
controlled by geology, climate, topography, surface The conversion of grassland to agroecosystem
activities and mineralogical composition of soil. The leading to loss of soil organic carbon is a major global
present study is focused on the comparative and challenge. Although the tropical soils are known to be
comprehensive assessment of hydrogeology and vulnerable to the loss of carbon due to land use change
geochemistry to examine pathways and chemometrics yet, limited information is available, especially in the
of the groundwater in the study area. Geochemical dry tropics. The present study was aimed to analyze
indices and multivariate techniques were applied to the impact of conversion of grassland to agroecosystem
assess the groundwater chemistry and potability of the in the dry tropics on the content of soil organic carbon,
water present in the study area. Groundwater samples soil carbon stock, bulk density and porosity. This study
were collected in two seasons (Pre-monsoon and Post- was conducted in the experimental plots of department
monsoon) and all the samples were analysed for the of botany, Banaras Hindu University. The soil organic
physico-chemical parameters and major ions. Majority carbon content was higher in the grassland (0.81%)
of the parameters were found well within the than the agroecosystem (0.57%). Same trend was
permissible limit except few chemical parameters such observed in the case of soil carbon stock and soil porosity
as pH, TDS and Total alkalinity at certain locations of whereas the trend of the bulk density was reverse. It
Vijaypur block of Samba district however all the may be suggested that to increase the soil organic
groundwater samples analyzed from Bhaderwah tehsil carbon and soil carbon stock in agroecosystems,
of Doda district reflects the values of chemical management practices involving higher organic matter
parameters well within the acceptable limits as per the input to the soil should be recommended so that the
IS10500. Carbonate weathering and cation exchange agroecosystem would lead not only to long term
capacity is important geochemical process responsible sustenance of soil fertility and productivity but also
for controlling the major ion distribution in the study sequester atmospheric carbon into the soil of dry tropics.
area. The present study reveals that Groundwater Key words: Carbon stock, Carbon sequestration, Dry tropics,
quality of Vijaypur block is suitable for drinking and Porosity, Soil organic carbon.

110 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SIV/P-2 herbal medicines containing spurious amounts of Pb

and Cd was predicted to cause health hazards in human.
Lead and Cadmium in Indian Herbal Key words: Cadmium, Lead, Herbal medicine, Hazard
Medicines: Health Risk Assessment assessment.

Debika Chakraborty, Arindam Nath, Parmita SIV/P-3

Bhattacharjee, Shamim Sultana Choudhury and
Suchismita Das* Environmental Impact Assessment of
Aquatic Toxicology and Remediation Laboratory, Proposed Residential Project: A Case
Department of Life Science and Bioinformatics, Assam Study of Agra
University, Silchar-788011, INDIA, Email: drsuchismita9
@gmail.com Manjul Gupta1*, Pankaj Kumar Srivastava 1 and
Around 70-80% world populations depended on Akash Kumar2 *
non-conventional, herbal medicine for their primary 1
CSIR-National Botanical Research Institute, Rana Pratap
healthcare. From the cultivation of these herbs to the Marg, Lucknow-226001, INDIA; 2PARAMARSH, Servicing
final herbal product, there are many factors which Environment and Development, Lucknow, INDIA, Email:
influence the quality of herbal medicines. Often, trace manjulnbri@gmail.com, drpankajk@gmail.com
elements, such lead (Pb) and cadmium (Cd) are
Environmental Impact Assessment (EIA) is a
reported in them. With the increased dependence and
planning tool generally accepted as an integral
use of herbal medicines, there is a growing concern
component of sound decision-making.EIA is to give
regarding the adverse health effects of long term
the environment its due place in the decision-making
consumption of these medicines. With this aim, we
process by clearly evaluating the environmental
estimated Pb and Cd levels in 25 commonly used
consequences of the proposed project activity before
marketed herbal medicines available with Ayurvedic
the action is taken. Early identification and
medicine sellers as well as 30 tribal medicines collected
characterization of critical environmental impacts allow
from Dimasa, Riang and Khasia tribes of North East
to assess the environmental acceptability of a proposed
India. In marketed products Pb levels ranged from
developmental project and conditions which should be
0.46±0.07 to 15.64±0.72 mg kg-1 and Cd levels ranged
applied to mitigate those impacts. Today we know that
from 0.41±0.06 to 4.5±0.68 mgkg-1 . There was
the building sector is developing very fast and
significantly less Pb (0.68±0.001 to 5.96±0.01 mg kg-
responsible for various environmental problems:
) and Cd (0.1±0.0001 to 0.84±0.005 mg kg-1) in tribal
pollution, waste, resource depletion, land use, biological
medicines, presumably due to the collection of samples
degradation etc. In India, building industry is growing
from pristine environment. The chronic daily intake
rapidly at a rate of9.2% as against the world average
(CDI) values of each medicine were calculated based
of 5.5%. The EIA study in this context is preparing a
on predictive risk assessment modelling; the tribal
detailed account of environmental impacts of proposed
medicines posed no imminent threats. However, the
activity so that appropriate interventions could be
CDI of Pb from marketed herbal medicines ranged
taken.An attempt has been made in the study for
from 0.00005-0.001 mg kg-1 bw/day, and 0.00007-
assessing environmental impacts of proposed residential
0.0003 mg kg-1 bw/day for Cd, some of which were
project in Agra city of UP, India by using checklist
above permissible limits set by WHO. Consequently,
methodology. The findings of the EIA Study have
high hazard quotient and hazard index (both more than
identified the nature and extent of environmental
1) were observed for these metals in some samples,
impacts arising from construction and operation of the
which might impart individual as well as cumulative
project. The results demonstrated that ‘Transportation
effects on the health. Thus, apart from other dietary
Resources’, ‘Noise Pollution’, Waste Generation,
as well as inhalation sources of contaminants (which
Sewage Pollution ‘Dust Generation with Construction
were not studied here), regular consumption of marketed
Machinery’ are the most risky environmental impacts

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

may arise due to the proposed residential project. current study reveals that the trend of organochlorine
Environment Management Plan is a practical and pesticides is decreasing in comparison to the past. The
achievable plan of management to ensure that any study concluded that the levels of metals and pesticide
environmental impact during all the phases is minimized residues are variable in different fish species but within
and lead in the direction of sustainable development. the maximum residue levels recommended at National
EMP for the above mentioned impacts are; provide and International standards.
separate entry & exit points and driveways, site Key words: Accumulation, Fish species, Heavy metals,
barricading, installed organic waste converter, frequent Pesticides
water sprinkling. In conclusion, results from EIA study
showed that the proposed residential development SIV/P-5
project has significant impacts on the environment.
Implementation of an effective EMP will minimize the Tree Falling Risk Assessment of IOCL
environmental issues occur during the project cycle. Campus New Bongaigaon, Assam and
Key words: Building, Construction, Environment, EIA, Impact. their Control Measures
SIV/P-4 Rehanuddin, Priyanka Agnihotri, P.S. Chauhan and
S.K. Tewari
Heavy Metals (Pb, Cd, Hg) and Org- CSIR-National Botanical Research Institute, Lucknow-
anochlorine Pesticides in Freshwater 226001, INDIA, Email: rehanfaruqui2117@gmail.com
Fishes: Risk Evaluation for Consumers Trees are important natural resources; they
provide us food, cloth, medicine, firewood, timber wood,
Geeta Singh beauty and shade also. An overall assessment of trees
present near at IOCL sites in Bongaigaon, Assam has
DDU Gorakhpur University, Gorakhpur-273009, Uttar
been done in order to identify the risk of falling
Pradesh, INDIA, Email: geetasanger@gmail.com
associated with them. In order to carry out the study,
Fishes are a valuable source of high-grade protein grouping of trees depending on their position, angle of
and nutritious component of the human diet. Heavy inclination and ageing were done. Few of them were
metals and OCPs from natural and anthropogenic found to be infected with different microbes, which
sources are released into aquatic ecosystems, where weakens them and makes them prone to fall. Several
they pose a serious threat because of toxicity, long approaches have been made to manage the risks from
persistence, bioaccumulation, and biomagnifications in trees which involved assessment of trees according to
the food chain. Fishes are badly affected because they the risk of their falling and causing injury, and further
are top consumers in aquatic life. Five most cutting of vulnerable trees alleviating the danger. Total
commercially important fresh water fish species were 72 trees are observed/studied at the study site, some
collected, digested and analyzed for organochlorine of which pose direct threat on pipeline of IOCL which
pesticide and toxic metals. The metal concentrations probably leads to bursting of the pipeline causing several
ranges of Pb, Cd and Hg detected were 0.02-18.0, hazards. Therefore “Tree Falling Risk Assessment”
0.05- 21.0 and 0.01-26.0 ppb respectively. Among the has been undertaken emphasizing the prevention of any
three metals, concentration of mercury was detected hazard. The parameters under consideration involve
highest. The fish samples were contaminated with one girth of the trees, their height, angle of inclination,
or more DDT analogue and HCH isomer residues number of trees infected by pests, termites, pathogen
respectively. pp-DDT and gamma-HCH was the most followed by their age. Biological treatment (Basically
frequently occurring residues in fish samples. The level Trichoderma and PSB) has been provided to eradicate
of contamination with pp-DDT ranged ND-48.0 ppb infection, while the trees carrying high potential of
and gamma-HCH from ND-68.2 ppb. Delta-HCH damage due to major degree of infection need to be
isomer was not detected in any of the fish sample cut for the safety purposes.
analyzed. Concentration of total DDT is higher than Key words: Trichoderma, PSB, Tree Falling Risk Assessment,
total HCH. The level of pesticides observed in the IOCL.

112 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Session V
Sub-tropical and Tropical Oak under
Global Change

Abstracts 27-30 November, 2018, NBRI, Lucknow 113

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

114 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)


Regeneration Ecology of Oaks Managing Regeneration Potential to

Sustain Oak Forests in North America
Albert Reif*
Albert-Ludwigs-University, Tennenbacher Str. 4, D-79085 Daniel C. Dey*
Freiburg, GERMANY, Email: albert.reif@waldbau.uni-
US Forest Service, Columbia, Missouri, USA, Email:
Deciduous oaks are important components of
Historical disturbance regimes, primarily fire-
temperate forests, providing many ecosystem goods
driven, promoted oak dominance and widespread
and services, including timber and firewood, acorns for
distribution for thousands of years throughout eastern
feeding, tannins from bark, and others. Several factors
North America and along the west coast of the USA.
are influencing the regeneration of oaks. The literature
Aboriginal populations used fire for subsistence living
mentions light supply, predation of acorns by rodents,
and in doing so increased oak abundance. Colonial
wild pigs and other acorn eating animals, browsing
settlement in the early Industrial Era intensified land
pressure by wild and domestic animals, infection of
use practices across broad landscapes, including
leaves with the mildew fungus Microsphaera
frequent burning, which promoted oak dominance along
alphitoides, competition with ground vegetation, late
a gradient of site qualities from xeric, low productivity
frost, and water supply. All these factors are interrelated
to mesic, high productivity sites. For the better part of
in a complex way. In terms of their life form strategy,
100 years, fires have been suppressed and practically
many deciduous oaks can be classified as stress-
eliminated across the range of oak in North America.
tolerating pioneers. It seems that germination and
Initially, oaks dominated forest recovery following
successful establishment of these oaks depend on an
colonial exploitation, but changing land use practices
open canopy and a low ground vegetation. In European
and modern disturbance regimes are accelerating the
cultural landscapes with closed forest cover and
replacement of oak by competing species that are better
intensive farming, this can be facilitated through wood
adapted to current conditions, especially on the more
pasture with cattle and sheep. Browsing is tolerated
productive sites. Today, there is a well-accepted
by the young oaks, when light supply is high. Under
recognition of oak regeneration failure and the
browsing pressure, oaks on open sites are able to
widespread loss of oak forests. Similar observations
survive, forming “bonsai-like” dwarfed deeply rooting
are being reported around the world. Regeneration
treelets. However, height growth only can start after
potential of a species is the probability that the species
the end of grazing. This can be achieved by temporal
will be competitive through regeneration and stand
and spatial fluctuations of browsing pressure. It seems
development to prevail in the mature stand. It is the
that deciduous oaks are best adapted to transitional
summation of a species regeneration potential from all
habitats, i.e. along forest edges, in hedges and
modes of reproduction, including current seed, seed
shrubland. In such habitats, establishment is successful
bank, new seedlings that establish after a regeneration
even under presence of browsing wildlife. Today,
disturbance, advance reproduction, and vegetative
arguments of nature conservation and climate change
reproduction from stump sprouting, root sprouting and
recommend an increased effort to facilitate oak
layering. Regeneration potential has been quantified
regeneration. For that, periods of grazing changing with
for some oak species. Large, competitive oak advance
periods of succession can be a successful approach,
reproduction and stump sprouts are essential to sustain
which was well known in the past, but is nearly
oak forests. Regeneration potential is influenced by
forgotten today.
initial floristics, site quality, competing vegetation,
Key words: Deciduous oak, Temperate climate, Wood herbivores, and level of management. Oak regeneration
pasture. potential in modern forests is commonly too low to
sustain oak stocking at current levels.
Recommendations are given for silvicultural

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

prescriptions designed to increase oak regeneration SV/O-1

potential to sustain oak forests.
Key words: Quercus, Silviculture, Ecology, Regeneration,
Assessing Restoration Potential of
Succession. Fragmented and Degraded Fagaceae
Forests in Meghalaya, Northeast India

Genetic Diversity, Hybridization and Prem Prakash Singh*1 , Anna Dermann2, Florian
Dermann 2 , Dibeyndu Adhikari 1 , Tamalika
Conservation of Mexican Oaks Chakraborty3, Purna B. Gurung 1 , Saroj Kanta
(Fagaceae: Quercus) Barik1,4, Jürgen Bauhus2 and Somidh Saha2,5
Department of Botany, North-Eastern Hill University, INDIA,
Ken Oyama* 2
Chair of Silviculture, University of Freiburg, Freiburg,
Universidad Nacional Autónoma de México (UNAM), GERMANY, 3Chair of Site Classification and Vegetation
National Autonomous University of Mexico, MEXICO, Email: Science, University of Freiburg, GERMANY, 4National
kenoyama@enesmorelia.unam.mx Botanical Research Institute, Lucknow, INDIA, 5Institute for
Technology Assessment and Systems Analysis, Karlsruhe
Mexican oaks (Fagaceae: Quercus) are one of Institute of Technology, Karlsruhe, GERMANY, Email:
the richest genera of trees with more than 160 species prem12flyhigh@gmail.com
described including 109 endemics. Mexico is considered
The montane subtropical broadleaved wet hill
one of the main centres of oak diversification in the
forests of Meghalaya are part of megadiverse biome.
world. Oaks are distributed in temperate and subtropical
It is situated at the transition zones between the
forests in heterogeneous environments along an
Himalayas and Indo-Burma biodiversity hotspots. Ten
altitudinal gradient of 0 to more than 2500 m asl. Oaks
tree species of Fagaceae family dominate these forests.
are considered key species in the ecosystems where
Fagaceae tree species are important for sustenance
they are distributed with an intricate net of interactions
of the local tribal populations. These forests are highly
with ectomycorrhizal fungi, insects, epiphytes, among
degraded and fragmented due to anthropogenic
others. A comparative analysis of population genetic
disturbances such as mining, unsustainable forest
diversity between selected oak species distributed in
utilization, shifting cultivation, and browsing. In past,
Mexico and other parts of the world indicated that
these forests originally formed large continuous areas
Mexican oaks harbor higher levels of genetic diversity
in the landscape. Today, numerous degraded and
but low genetic differentiation. Hybridization between
fragmented forest patches are scattered over the entire
different oak species can explain in part this high levels
landscape. In this study, we assessed for the first time,
of genetic diversity. Patterns of oak hybridization are
the restoration potential i.e. capacity of natural
presented in order to show the different modes and
regeneration and state of vitality of Fagaceae species
number of oak species involved. Conservation of oak
in Meghalaya. The main objective was to understand
forests in Mexico is urgent due to the high deforestation
how different biotic factors, abiotic factors, and
rates and the fragmentation of remnant natural forests.
anthropogenic disturbances influence restoration
Key words: Genetic differentiation, Mexican Oak, Population potential of Fagaceae species. We selected two
genetic diversity. Lithocarpus, four Castinoopsis, four Quercus species
and fragmented forest patches in six locations on an
elevational gradient between 800 - 2000m asl along
the south-facing slope of Khasi Hills, Meghalaya. We
found the degraded forests still harbour high tree species
richness (143 species). Additionally, our study showed
that the plant community was heterogeneous in
composition, structure, and distribution. We found a
general trend that the Fagaceae family had high natural

116 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

regeneration potential i.e. a high number of seedlings entropy (MaxEnt) model using field observations and
and saplings but low recruitment of adult trees. The literature generated occurrence locations and CMIP5
vitality of Fagaceae species was poorer than non- (Couple Model Inter-comparison Project) derived
Fagaceae species because of high anthropogenic bioclimatic variables were used for the study. The
disturbance to Fagaceae species. This case study will predictions were done on the geographic area of
help us in enhancing our understanding of the status of Uttarakhand according to four IPCC RCPs for the
these forests and possible restoration strategies for these future periods 2050 and 2070. Our results show that
degraded and fragmented forests patches of the estimated potential area of the species is likely to
Meghalaya. have mixed results in future for all climate change
Key words: Forest Landscape Restoration, Quercus, scenarios for Quercus in its actual habitats. Shifts in
Castanopsis, Lithocarpus, Forest, Regeneration, geographic distributions of climatic niches reflect
Anthropogenic Disturbances. unusual patterns, implying the need for climate adaptive
habitat restoration. Solutions intended need to be
SV/O-2 climate resilient restoration of degraded patches using
in-situ conservation measures.
Assessing the Impacts of Climate Keywords: Oak forests, Central Himalayas, Uttarakhand,
Vulnerability on Quercus Habitats in Climate Change, Species Distribution Models
Central Himalayas: Future SV/O-3
Status of Oaks and Related Species
Shalini Dhyani* and Rakesh Kadaverugu for Management and Conservation
CSIR-National Environmental Engineering Research
Institute, Nagpur-440020, Maharashtra, INDIA, Email:
Yangchenla Bhutia1,2*
Oak forests in Central Himalayas represent 1
Suri Sehgal Centre for Biodiversity and Conservation,
climax between 1000-3500 m amsl. Five species of Ashoka Trust for Research in Ecology and the Environment,
oaks viz. Quercus glauca, Q. leucotrichophora, Q. Jakkur Post, Royal Enclave, Sriramapura, Bangalore-560064;
floribunda, Q. lanata and Q. semecarpifolia grow Karnataka, INDIA; 2Manipal Academy of Higher Education
naturally and successively replace other species along (MAHE), Manipal-576104, INDIA, Email:
the increasing elevation in stated order. Oak dominated yangchenla.bhutia@atree.org
mixed broadleaved forests not only provide efficient
The oaks and its related tree species form the
provisioning ecosystem services but are also source of
dominant composition of temperate forests. They
regulating, supporting and cultural ecosystem services
contribute significantly to overall functioning of forest
in the region. Oak forests are proven for their high
ecosystem. Besides, they also have an intricate history
water holding capacity, sustaining healthy watersheds
with humans for its different uses. But, these species
and spring sheds. Deforestation in last few decades
are globally threatened, and the Himalayan oaks are
has led to change in succession patterns and biodiversity
no exception. Recent studies from the Eastern
loss due to accelerated invasion of Chir Pine (Pinus
Himalayan region of Sikkim reported forest degradation
roxburghii) in oak forests. Cumulative impact of human
particularly concentrated in the oak forests. In the
interferences coupled with climate change has been
current study we evaluated the diversity, structure,
significant on oak dominated forests. Conservation of
forest composition of tree species, including the
oak forests is a socio-ecological challenge that requires
regeneration status of oaks and their related species
an interdisciplinary approach. The present study
along the altitudinal gradient. We covered three forest
focuses on assessing the climatic niche of Quercus
types ranging from 900m to 3200m above sea level,
species and understanding its shift in the future
the potential distributions zone of oak and its related
distributions due to climate change. The Maximum
species. Our result reported oaks and its related species

Abstracts 27-30 November, 2018, NBRI, Lucknow 117

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

as the major contributor to the overall forest ecosystem over-exploitation and growth of invasive species. About
with highest Importance Index Value. However, an 78 species of oaks are listed as threatened. Himalayan
exhaustive analysis showed a more nuanced condition. oak forests are under serious threat and are being
One of the major concerns was the size class invaded by pine forests due to the increase in
distribution. Overall the oaks and the related species temperature. The species growing as associates of pine
were predominantly of large diameter. However, the may cross the frontiers of pine forests, and become
low elevation forests completely lacked trees above new elements of the oak forests. In most of the oak
80cm diameter. It is feared under current situation the species, seed (acorn) germination and seedling survival
oak forests will disappear with unsustainable loss. We are very poor. It is reported that only one in 10,000
suggest conservation strategies focusing on of different acorns results in an eventual tree.This critical issue
stages of plant growth to ensure perpetual functioning prompted us to study the seed germination, seedling
of forest ecosystem. survival and growth in two species of Quercus (Q.
Key words: Forest composition, Conservation strategies, dealbata and Q. griffithii) growing in northeast India.
Regeneration status. Seeds of these two species vary widely in mass and
can be categorized into light-, medium- and heavy-
weight classes. Protein, carbohydrate, lipid and energy
contents were greater in heavy seeds than in the
medium-weight and small seeds. The heavy seeds
emerged earlier and showed higher germination
Effect of Microsite Characteristics on percentage than small seeds. Further, the seedlings
Seed Germination, and Survival and emerged from heavy seeds performed better in terms
Growth of Seedlings of Two Species of survival and growth. Of the four substratum
microsites tested (T 1 - herbaceous vegetation was
of Quercus Growing on the Hills of allowed to grow and litter was left undisturbed; T2 –
Northeast India herbaceous vegetation was allowed to grow, but litter
was removed; T3 - herbaceous vegetation was cleared
M.L. Khan1* and R.S. Tripathi2 but litter was left intact; T4 – both litter and herbaceous
Department of Botany, Dr. Harisingh Gour Vishwavidyalaya vegetation were removed), emergence, survival and
(A Central University), Sagar-70003, MP, INDIA; 2Ex-INSA growth of seedlings were highest in the T4 plots and
Senior Scientist, CSIR - National Botanical Research Institute, lowest in T1 plots. Further, seedling emergence, survival
Rana Pratap Marg, Lucknow-226001, INDIA, Email: and growth were more adversely affected by the
khanml61@gmail.com herbaceous cover than the litter. The occurrence of
The genus Quercus (oak) is native to the northern seeds of different weights showing large variation in
hemisphere and extends from cold latitudes to tropical food reserves and energy content in two studied oak
Asia and the Americas. Oaks are considered as species, and differential response of these seeds and
keystone species in a wide range of habitats from seedlings produced by them to microsite characteristics
Mediterranean semi-desert to subtropical rainforest. may be viewed as an important regenerative strategy.
Quercus species are considered humus-creating The findings of this study have considerable implication
machines, house for many animals, source of free for the silviculture and management of oak forests.
livestock feed and creator of microclimates. However, Key words: Quercus spp., Seed mass variability, Seed
many species of oaks are under threat of extinction in germination, Seedling survival and growth, Microsite
the wild due to destruction of their natural habitats, characteristics.

118 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SV/O-5 and L. pierreioides, sp. nov. from Cambodia. The

photographs, illustrations, DNA barcode sequences,
A Taxonomic Study of Lithocarpus and the description of preliminary conservation status
(Fagaceae) in Vietnam Based on are also provided for the new species. Additionally, eight
species are lectotypified in this study.
Molecular Phylogeny and
Key words: Fagaceae, Lithocarpus, MIG-seq, Phylogeny,
Morphological Observations Taxonomy, Vietnam.

Nguyen Van Ngoc1,2*, Hoang Thi Binh1,2, Shuichiro SV/O-6

Tagane1, Hironori Toyama1, Keiko Mase1, Chika
Mitsuyuki3, Joeri Sergej Strijk4, Yoshihisa Suyama3 Forecasting Climate Change Impact
and Tetsukazu Yahara1 on Distribution Range of Fagaceae
Kyushu University, Fukuoka, JAPAN; 2Dalat University,
Dalat City, VIETNAM; 3Tohoku University, Osaki, Miyagi,
Members in Meghalaya using Open
JAPAN; 4Guangxi University, Nanning, Guangxi, CHINA, Source Data
Email: ngocnv@dlu.edu.vn
Lithocarpus Blume is the second largest genus Dibyendu Adhikari 1 *, Prem Prakash Singh 1 ,
of the family Fagaceae, with approximately 350 species Raghuvar Tiwary1 and Saroj Kanta Barik1,2
in the world. The centre of species diversity is mainly Department of Botany, North-Eastern Hill University,
in continental Southeast Asia, especially in China (123 Shillong-793022, INDIA; 2CSIR-National Botanical Research
spp.) and Vietnam (117 spp.). In Vietnam, almost all Institute, Lucknow-226001, INDIA, Email: dibyenduadhikari
the species of Lithocarpus (Fagaceae) were described @gmail.com
by French botanists until 1920s and some others were Members of Fagaceae with 8 genera have
until 1950s. Since then, the taxonomic inventories of important functional roles in forest ecosystems. The
Lithocarpus in Vietnam were disrupted until recently. economic value of oaks and beech are well recognized
In this study, our morphological observations and since ages, which are used as edible nuts, timber, floor
molecular phylogenetic analyses based on both classic wood, furniture, fodder and wine barrels as well as
DNA sequencing of two cpDNA (rbcL and matK) corks from bark. They are found in a range of climatic
and one nrDNA (ITS) regions and multiplexed inter- conditions in the northern hemisphere. The growing
simple sequence repeat genotyping by sequencing anthropogenic and climatic impacts are bound to
(MIG-seq) were employed to revise three complexes influence their distribution range. Therefore, estimating
of Lithocarpus in Vietnam: L. elegans complex, L. their climatic niches and predicting the current as well
hancei complex, and L. vestitus complex. The NJ tree as future geographic distribution should help in
based on MIG-seq data clearly provide the evidence developing appropriate strategies for their long-term
of species delamination. After a careful examination conservation and management. We attempted to
based on both these phylogenetic data and reconstruct the climatic niche of 10 Fagaceae species
morphological comparison using our newly collected in Meghalaya using population survey and ecological
specimens, taxonomic literature, type specimen images niche modelling. The climatic niches of these species
available on the website, we distinguished five species were estimated using the data on current climatic
in the L. elegans complex, three species in the L. conditions. The reconstructed niches were then
hancei complex, 13 species in the L. vestitus complex. projected to various climate change scenarios
Keys to the species of each three complex are represented by different climate models pertaining to
provided. Through our study, nine new species are the time frames of 2050 and 2080. The bioclimatic data
recognized and described: L. bidoupensis, sp. nov., L. for current conditions as well as change scenarios with
chinhii, sp. nov., L. dahuoaiensis sp. nov., L. a spatial resolution of ~ 1 km were downloaded from
hongiaoensis, sp. nov., L. pseudoannamensis, sp. www.worldclim.org. The modelled climatic niches and
nov., and L. vuquangensis, sp. nov. from Vietnam, predicted geographic range of the selected species in
and L. bokorensis, sp. nov. L. monoromensis, sp. nov., the present study conform to the information available

Abstracts 27-30 November, 2018, NBRI, Lucknow 119

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

in the literature in respect of a few species. Future assessed on the mortality of the trees. Climate-growth
distributional ranges as predicted under different climate relations (fifty years), resistance, recovery, and
change scenarios show that some species will lose resilience (for multiple droughts) were quantified. The
climatic suitability, while in case of some other species, retrospective ecophysiological analysis was performed
previously less suitable areas will become more by using stable C and O isotopic signatures of the tree
conducive for species persistence. However, certain rings. We found, increasing AWC, light, plant-plant
areas will retain climatic suitability. We suggest that interactions, and tree species diversity reduced
such areas may be useful for long-term conservation mortality. Beech trees died when the dead
of oaks. Appropriate silvicultural practices should be aboveground-biomass crossed a threshold of 58%. The
developed in these areas to counter the climate and dependency of tree growth to AWC became higher
anthropogenic pressure. with increasing magnitude of drought. Recovery and
Key words: Fagaceae, Ecological niche, Climate change, resilience were higher in trees with higher AWC, but,
Geographic distribution. opposite for resistance. Dual isotopic analyses revealed
that beech trees growing in lower AWC plots showed
SV/O-7 a higher response to drought, climatic dependency, and
higher stomatal resistance compared to those growing
Temperate Broadleaved European in higher AWC; later showed higher stomatal
Beech (Fagus sylvatica L.) Trees are conductance. We conclude that: 1) beech trees growing
on dry sites have a high ecological plasticity to drought;
Showing High Ecological Plasticity however, died after crossing a threshold of dead
under Multiple Drought Events aboveground-biomass; 2) high tree species diversity in
dry sites could mitigate drought stress in beech trees;
Tamalika Chakraborty1*, Somidh Saha2,3, Andreas and 3) changes in soil water condition even at microsite
Matzarakis4 and Albert Reif1 level could alter the growth and survival strategies of
1 beech trees. This study will enrich the state of the
Chair of Site Classification and Vegetation Science, Faculty
of Environment and Natural Resources, University of knowledge about the ongoing debate on the vulnerability
Freiburg, Tennenbacherstr. 4, D-79106 Freiburg, GERMANY; of beech trees to drought in Europe.
Institute for Technology Assessment and Systems Analysis, Keywords: Available Soil Water Storage Capacity, Biomass,
Karlsruhe Institute of Technology, Karlstr. 11, D-76133 C and O Stable Isotopes in Tree Rings, Dendroecology, Plant-
Karlsruhe, GERMANY; 3Chair of Silviculture, Faculty of plant interactions, Tree mortality.
Environment and Natural Resources, University of Freiburg,
Tennenbacherstr. 4, D-79106 Freiburg, GERMANY; 4Centre SV/O-8
for Medical Meteorological Research, German Weather
Service, Stefan-Meier-str. 4, D-79104 Freiburg, GERMANY, Mapping of Ecosystem Services as
Email: tamalika.chakraborty@waldbau.uni-freiburg.de,
chakraborty.tamalika@gmail.com Sustainable Management Tool – A
Droughts in central Europe have been increasing Case from Oak Forests in Western
due to rising temperature and shifting precipitation. We Himalayas, India
studied the cause and threshold of drought-driven
mortality and survival strategies of beech trees, which Sabyasachi Dasgupta1,2 *, Subhasis Mahato1 and
is the most important broadleaved tree species of central N.P.Todaria1
Europe. The study was conducted in five near-natural 1
Department of Forestry and NR, H.N.B. Garhwal University,
dry forests in Germany and Switzerland. Tree-level Srinagar (Garhwal), Uttarakhand-246174, INDIA; 2
mortality (as a percentage of dead aboveground- Department of Forestry and Biodiversity, Tripura University,
biomass) and available soil water storage capacity Agartala, Tripura-799022, INDIA, Email: sdhnbgu@
(AWC) were quantified. Influences of biotic (plant- gmail.com
plant interactions, tree species, structural diversity) and
Through out the world oak species have
abiotic (light, AWC, soil pH, bulk density) factors were
considerable economic and conservation significance.

120 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

However, the Garhwal Himalayan Oak forests provide Institute of Technology (KIT), Karlsruhe, GERMANY;
various ecosystem services and are not considered for Institute of Geography and Geoecology (IfGG), Karlsruhe
their timber value. The present study was desired to Institute of Technology (KIT), Karlsruhe, GERMANY,
explore the spatial mismatch in the delivery of Hooman Latifi, Khajeh Nasir University, Tehran, IRAN,
Email: e.shafeian90@gmail.com, fabian.fassnacht@kit.edu,
ecosystem services by oak forest ecosystems and also
to understand the dynamics and future of oak in the
Himalayas. An oak dominated forest site was selected An alarming decrease in oak forest habitats has
in Rudraprayag district of Uttarakhand, India with an been recently reported throughout the world. Oak
average elevation of 2400m above sea level. The stock decline has also been observed in the Zagros mountain
and flow data were mapped using polygon approach area in Iran. Identifying and mapping declining trees is
with the help of GIS techniques. Contribution of different an important step to manage the spread of infestations.
species to total forest biomass significantly varied. The Several earlier studies have demonstrated the potential
biomass extraction was disproportionate to availablility of remote sensing for such purposes, however, there
of Quercus semecarpifolia and Quercus are still open questions concerning why certain declining
leucotrichophora in that particular forest. Flow of areas can be detected with remote sensing and others
ecosystem services in the form of fuel wood and green not. Moreover, mapping declined areas is just the first
fodder was variable in spatio-temporal scale. In flow step towards understanding the reasons for forest
and stock map, it was clearly visible that the flow of decline. Once the affected areas have been identified,
fuel was more in lower altitudinal zone whereas fodder it is necessary to understand the underlying drivers that
flow was high in the mid and high altitudinal zones. have caused the decline. In this paper, we will review
The availability of fodder was less near the village area the current literature on oak dieback, with a specific
because of more fuel and fodder extraction in the past. focus on Iranian Oak forests and the current status on
Therefore, if extraction is not controlled, the mid and the application of remote sensing data to understand
upper elevation oak forest will be soon degraded. Spatial and map patterns and processes related to oak decline.
dynamics showed that as we move away from the This review will provide the basis for a subsequent
settlement, the realization of ecosystem services by remote sensing study consisting of four steps: Initially,
the people also decreases. It is likely that extraction in the minimum size of declined forest patches that are
a planned manner with the help of stock and flow map detectable with current multi-spectral satellite data, will
will drastically reduce the unsustainable extraction be identified. Next, the influence of co-occurring land-
patter and help in sustainable measure of a natural oak cover classes on these results will be assessed by
forest. applying a multi-scale remote sensing approach
(including very high-resolution drone imagery and multi-
Key words: Flow and stock map, Ecosystem Services, Oak
forest, Biomass, Himalaya.
spectral satellite data). Then, the derived spatial patterns
of declined forest areas will be analyzed in relation to
SV/O-9 a range of explanatory variables. Ultimately, dendro-
chronological time-series of stress events will be
A Review of Forest Decline with a compared to spectral information available from historic
Landsat datasets to check the potential of reconstruc-
Special Focus on the Oak Forests in ting stress events over time using satellite data.
Iran Key words: Oak Decline Review, Multi-scale Remote Sensing,
Dendro-chronology, Persian Oak, Forest vitality.
Elham Shafeian1*, Fabian Ewald Fassnacht2 and
Hooman Latifi3
DAAD awardee and future PhD student at Karlsruhe

Abstracts 27-30 November, 2018, NBRI, Lucknow 121

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SV/O-10 of nuclear ITS and seven cpDNA loci was undertaken.

The sequence analysis revealed that although much
ITS and cpDNA Loci Based Molecular sequence variations existed in various studied loci.
Phylogeny of Fagaceae Failed to However, the complete tree topology was not
successfully resolved in maximum parsimony,
Resolve the Backbone Topology maximum likelihood and Bayesian method of inference
used in phylogenetic analysis. Therefore, a few loci
Babita Joshi 1* , Gopal Ji Tiwari 1 , Kiran1, Prem based phylogeny will not end up with clear cut species
Singh2, Dibyendu Adhikari2, Satya Narayan Jena1 delimitation with correct backbone topology in
and Saroj K. Barik1,2 Fagaceae. This necessitates a novel standalone
Plant Molecular Genetics Lab, CSIR-National Botanical approach to resolve the topology issues in phylogentic
Research Institute, Rana Pratap Marg, Lucknow-226001, lineage.
INDIA; 2Department of Botany, North-Eastern Hill University,
Umshing Mawkynroh, Shillong-793022, Meghalaya, INDIA, Key words: Fagaceae, ITS, cpDNA, Phylogenetic
Email: babitajoshi051991@gmail.com relationship, Topology, Meghalaya.

The family Fagaceae is one of the largest groups SV/O-11

within Fagales, with a total of nine genera and
approximately 900 species occurring in diverse habitats Oak Decline and the Changing Face
throughout the northern hemisphere. The temperate of the Central Himalayan Forests
and semi-arid flora of north and central America is
rich in Quercus while warmer and mesic forest of Rajesh Thadani, Anvita Pandey* and Vishal Singh
south-east Asia is diverse in Lithocarpus and
Castanopsis. The wind-pollinated genus, Quercus has Centre for Ecology, Development and Research (CEDAR),
201/1 Vasant Vihar, Dehradun-248006, Uttarakhand, INDIA,
been extensively studied from its population genetics
Emails: rajesh@cedarhimalaya.org, anvita@cedarhimalaya
prospective. However very little study has been done
.org, cedarhimalaya@gmail.com
on broader phylogenetic relationship of Quercus as well
as the tropical and subtropical insect-pollinated The decline of oak forests of the central Himalaya
members of Fagaceae such as Lithocarpus, has for long been flagged as a major environmental
Castanopsis, Castanea, and Chrysolepis. In problem in the region. Forests of Banj oak (Quercus
taxonomy, the latter four insect-pollinated members of leucotrichophora) in particular have seen a significant
Fagaceae have similar flowers with same number of reduction in area and degradation of quality. A result
stamens and nectariferous pistillode. This makes it of this decline has been the increase in extent of chir
difficult considering the rest of the Fagaceae and its pine (Pinus roxburghii), particularly in the oak zone.
sister group with wind pollinated flowers for phylogeny Pine has lower socioeconomic value and supports lower
evaluation. In Quercus, natural hybridization and biodiversity and ecosystem services compared to oak.
introgression is also an important aspect with respect While oak decline is largely the consequence of
to the formation of new species or increasing genetic excessive anthropogenic disturbance, the mechanism
variation within species. Besides these, many species remains poorly studied. This paper focuses on lopping
of Quercus exhibit variable phenotypes that overlap and litter removal and their impacts on Quercus
with other species. Thus, the whole broader leucotrichophora. Data from 40 permanent plots of
phylogenetic lineage in Fagaceae remains to be 400m2 each, and across a disturbance gradient, indicate
resolved. In India, Fagaceae is represented by four that diameter growth is adversely impacted by human
genera viz., Quercus, Lithocarpus, Castanea and disturbance. The annual growth in basal area of oaks
Castanopsis. These are distributed mostly in Indo- is almost five times higher in undisturbed plots as
Burma and Himalyan region. With an aim to understand compared to degraded plots. Undisturbed oak forests
the molecular phylogenetic lineage of the four major had over three times the Leaf Area Index (LAI)
genera of Fagaceae including 10 species collected from compared to degraded forests. The opening up of the
the state of Meghalaya, India, the sequence analysis canopy also gives an opportunity for chir pine, a light

122 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

demanding species, to establish. Litter removal leads Key words: Oaks, Lithocarpus dealbatus, Quercus griffithi,
to poor acorn germination as exposed acorns get nitrogen resorption.
desiccated and become unviable. Understory
disturbance is also evidenced in soil nutrient levels, and SV/O-13
soil carbon was over 3% in undisturbed stands but under
1% in disturbed and degraded forests. Collection of
How Important are Oaks and Other
biomass from oak forests through an opening of canopy, Fagaceae Members in Undisturbed
and changes in regeneration patterns encourage the Sacred Subtropical Wethill
ingression of pine at the expense of oak. This is further
exacerbated by climate change which leads to Broadleaved Forests of Meghalaya,
increased drought period and thereby vulnerability to India?
fire. An increased frequency of fire further benefits
the fire tolerant pines thereby accelerating oak decline. S. Kharbhi1*, R.R. Gogoi1, B.R. Suchiang1, I.M.
Key words:Oak-decline, Central Himalaya, Lopping, Litter Nonghuloo1, K. Upadhaya1 and S.K. Barik2
removal. 1
Centre for Advanced Studies in Botany, North-Eastern Hill
University, Umshing, Shillong-793022, INDIA; 2CSIR-
SV/O-12 National Botanical Research Institute, Rana Pratap Marg,
Lucknow-226001, INDIA, Email: serrada2020@gmail.com
Nitrogen Resorption Efficiency of Two Most oak (belonging to genus Quercus) and other
Oak Species in a Montane Sub- Fagaceae (belonging to the genera Lithocarpus and
Tropical Forest of Meghalaya Castanapsis) species of north-eastern India are now
confined to subtropical wet hill broadleaved forests of
Namita Thapa1 * and Saroj Kanta Barik1,2 the region. The members of Fagaceae are best
represented in the undisturbed sacred forests of
Department of Botany, North-Eastern Hill University,
Meghalaya, India. These species provide a number of
Shillong-793022, Meghalaya, INDIA; 2CSIR-National
Botanical Research Institute, Lucknow-226001, INDIA,
ecosystem services such as checking soil erosion and
Email: namita_2424@yahoo.in, sarojkbarik@gmail.com nutrient leaching, providing natural habitat and feeds
to wild animals, and maintaining nutrient cycling in the
Leaf nutrient resorption before senescence is forest and adjacent agroecosystems. The seeds of many
considered an important mechanism for survival and Fagaceae members are one of the most important feeds
growth of plants in low nutrient environment. The for wildlife as well as human being. In addition, oaks
process is important as it directly influences the leaf with their large crown, bole and deep root system, play
litter quality, thereby affecting the overall forest an important role in regulating the water cycle and
productivity. In the present study, N- resorption in two carbon sequestration. Fagaceae contributes
oak species belonging to two different functional groups, significantly to the tree diversity of subtropical and
viz. Lithocarpus dealbatus, an evergreen tree species, temperate forests. The present article quantifies the
and Quercus griffithi, a deciduous tree species was relative contribution of various oak and other Fagaceae
studied in a montane subtropical forest of Meghalaya. species to total tree diversity in three undisturbed sacred
Labelled nitrogen (15N) was used to understand the forest stands of Meghalaya. In the study sacred forests
pattern of nitrogen uptake and resorption in the two viz, Nongbah, Ialong and Mawnai, a total of 10 oak
oak species. N- resorption efficiency of Q. griffithi and other Fagaceae species were recorded belonging
was 44.73±0.63 %, while that of L. dealbatus was to the genera of Castanopsis, Lithocarpus and
32.21 ±0.37 %. A higher foliar resorption efficiency of Quercus. In Nongbah sacred forest, Castanopsis
labelled nitrogen (15N) was also observed in Q. griffithi tribuloides contributed about 30% to the total tree
(65.61 ±1.55 %) than in L. dealbatus (54.53±1.21%). importance value (IVI 87.05), followed by Castanopsis
These results suggest that N- resorption was better in purperella (IVI 19.97). The dominant species in terms
the deciduous oak species than the evergreen species. of IVI value in Ialong sacred forest were Castanopsis

Abstracts 27-30 November, 2018, NBRI, Lucknow 123

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

tribuloides (13.63), Quercus serrata (6.36) and determining the mean biomass value for each branch
Quercus griffithii (5.71). In Mawnai sacred forest, diameter class. The total branch biomass under each
Quercus griffithii (IVI 6.60), Castanopsis kurzii branch diameter class was determined by multiplying
(4.46) and Quercus semiserrata (3.72) were the mean branch biomass value for a particular branch
dominant. The tree population structure in each of these diameter class with the respective length of the
stand revealed that Fagaceae members constituted branches. The total branch biomass value was obtained
more than 90% in the higher DBH class trees. The by summing the biomass of the three branch diameter
contribution of Fagaceae members to total carbon stock classes. The mean leaf biomass was estimated for each
of the forest was very high due to their dominance in tree by taking leaf samples from three random branches
higher girth class trees. In view of their ecological and from each branch diameter class, and the total leaf
economic importance, oak forests should be prioritized biomass per tree was estimated by multiplying the total
for conservation. number of branches of the tree under each branch class.
Key words: Oaks, Importance Value, Sacred forests, Carbon The bole biomass was estimated using volume equations
stock. based on the DBH. The best fit model for volume (V)
estimation was of the form “V=a+bX, where X is DBH
SV/O-14 (R2=0.96). The mean bole volume per tree was 0.29
m3. The model can be applied to estimate biomass/
Estimating Carbon Stock in carbon and volume of the species in the diameter range
Lithocarpus dealbatus by Developing of 11-34.8 cm.

Species-Specific Allometric Models Key words: Lithocarpus dealbatus, Above ground biomass
(AGB), Carbon sequestration, DBH, north-eastern India.
Rashmi R. Gogoi 1 *, K. Upadhaya 2 and S.K. SV/O-15
Department of Botany, North- Eastern Hill University, Conservation of Oaks: From the
Umshing, Shillong-793022, INDIA; 2Department of Basic
Sciences & Social Sciences, North-Eastern Hill University,
Perspective of a Wood Anatomist
Umshing, Shillong-793022, INDIA; 3CSIR-National Botanical
Research Institute, Lucknow-226001, Uttar Pradesh-226001, Prachi Gupta1 * and Sangeeta Gupta2
INDIA, Email: rasrekh.aj@gmail.com 1
Wood Anatomy Discipline, FRI, Dehradun, INDIA, Email:
prachi.gpta@gmail.com, sangeeta.fri@gmail.com
Oak forests have high potential for carbon
sequestration. For carbon stock estimation both at Natural disturbances caused by climate change,
species and ecosystem levels, it is desirable to have rising temperature and forest fire coupled with
species-specific allometric models, since each species indiscriminate anthropogenic activities such as lopping,
varies in its carbon sequestration potential. Lithocarpus free grazing of cattle, litter removal, fuelwood and
dealbatus being one of the dominant oak species in fodder collection have adversely affected oak forests
the montane subtropical forests of north-eastern India, in the Himalayas. Secondary xylem is indispensable
biomass model for the species is crucial for carbon for water conduction, storage and providing mechanical
pool assessment of these oak forests. The above ground support to the tree, yet the knowledge of its
biomass (AGB) and carbon values were estimated by microstructure is hardly taken into consideration while
harvesting 17 trees in the diameter at breast height formulating conservation strategies. In the present study,
(DBH) range of 11.0 - 34.8 cm. AGB of each tree the wood microstructure of Quercus leucotrichophora
was determined by summing the biomass values for and Q. acutissima was analysed in detail as per the
branches, leaves and bole. The biomass for branches terminology given by the International Association of
was determined separately for three branch diameter Wood Anatomists, taking samples from its naturally
classes i.e. < 5 cm, 5-10 cm and > 10 cm. Three occurring areas as well as those planted in FRI campus.
replicate samples from each diameter class having 15 Q. acutissima is a deciduous oak found in the eastern
cm length was oven dried till constant weight for Himalayas where snowline is at a higher altitude and

124 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

temperature and rainfall are high compared to the oak. The results of the analysis reveal that potential
western Himalayas which is dominated by evergreen oak cover in the absence of competition and disturbance
oak, Q. leucotrichophora. The study revealed that can be 63% more than its present distribution in the
these two species of oak occurring in contrasting study area. The regeneration pattern of oak was studied
environment at higher altitudes are anatomically through a structured field survey in five vegetation
different and both maintained their respective wood formations (grasslands, pine, pine–oak, open oak, and
microstructure even when planted at lower elevations dense oak), representing a theoretical successional
in Dehradun (668 m). Since wood anatomy is a highly sequence from early- to late-successional stages.
conservative feature and doesnot change in different Uniformly distributed sampling plots were used to
environmental conditions, the plants are thriving well gather information about the vegetation communities.
in Dehradun too. The present study suggests that these The proportion of oak regeneration was substantially
oak species have potential for ex-situ conservation at higher in sites which had not witnessed forest fires in
lower elevations and can be a part of botanical gardens the recent past. Our results show that the density of
and urban forestry as well. oak seedlings regenerating in early successional pine
Key words: Degradation, Wood microstructure, Lower forests was close to 900 seedlings per hectare, however
elevations, Ex-situ conservation. more than 75% of these regenerating seedlings were
grazed. Additionally, overexploitation of existing oaks
SV/P-1 trees via lopping affected the structural complexity of
the forest canopy, thereby increasing light availability
Regeneration and Potential on the ground and supporting the regeneration of light-
Distribution of Late Successional demanding early successional pine species, as opposed
to the desired oak. We conclude that with strategic
Quercus leucotrichophora (Banj Oak) management that restricts the current anthropogenic
in the Central Himalayan Forests of disturbances, the extent of oak forest in the study area
can be increased.
Key words: Himalaya, Succession, Oak, Regeneration,
Niyati Naudiyal * and Joachim Schmerbeck 2 Distribution.
School of Environment and Natural Resources, Doon SV/P-2
University, Mothorawala Road, Kedarpuram, Dehradun-
248001, INDIA; 2Chair of Silviculture, Institute of Forest
Sciences, Faculty of Environment and Natural Resources,
Altitudinal Distribution of Oak Species
University of Freiburg, Tennenbacher Str. 4, 79085 Freiburg, in Three Wildlife Sanctuaries in
GERMANY, Email: naudiyal.niyati@gmail.com, joachim.
Sikkim, India
We studied the potential distribution of Quercus Arun Chettri and Subhankar Gurung*
leucotrichophora (oak) and factors influencing its Department of Botany, Sikkim University, 6th mile, Tadong,
regeneration in mid-montane forests of Central Gangtok, INDIA, Email: achettri01@cus.ac.in
Himalaya. Oak forests (covering 38.5% of total 57516
ha of the study area) play an important role in The present study aimed to understand the
maintaining the local livelihoods and forest ecosystem altitudinal distribution of oak species in Maenam Wildlife
services (FES) in the region. The potential distribution Sanctuary (2100-3300 m) in South Sikkim, Fambonglho
of oak in the study area was identified using the Wildlife Sanctuary (1500 – 2200 m) in East Sikkim and
ecological niche modelling tool, MaxEnt. Presence Bersay Rhododendron Sanctuary (1900-3800 m) in
location of oak along with bioclimatic and physiographic West Sikkim. These sanctuaries are predominantly
spatial data derived from WorldClim and digital dominated by Oak species. Associated species such
elevation model (DEM), respectively, were used as as Daphniphyllum himalayense, Eurya japonica,
input variables for identifying the fundamental niche of Symplocos theifolia, Rhododendron arboreum, R.

Abstracts 27-30 November, 2018, NBRI, Lucknow 125

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

griffithianum, Lyonia ovalifolia, Acer caudulatum of Castanopsis varied between 2.25 and 2.55 pg/2C.
grows along with oak species. At least 11 species of The genome sizes of these Castanopsis species are
oak have been reported from Sikkim by ENVIS, Sikkim. greater than that of Quercus species (range:- 1.88 to
The present study reports the distribution of nine oak 2.00 pg/2C). The genome size was used for
species in the study sites which fall under three genera. seggregating the three species. The three species had
The distribution of genus Castanopsis spp. ranged from significantly different genome sizes. This indicates the
1500 – 2400 m, Lithocarpus spp. from 1500- 2300 m successful application of flow cytometry results in
and Quercus spp. between 1500 – 2600 m at all the seggregating the elements within a taxa.
three sites. The oak species are: C. hystrix, C. Key words: 2C DNA content, flow cytometry (FCM),
lanceofolia, C. tribuloides, C. indica, L. Castanopsis, Fagaceae, Genome size.
pachyphyllus, L. elegans, L. fenestratus, Q.
lamellosa and Q. thomsoniana. SV/P-4
Key words: Wildlife sanctuary, Oak species, altitudinal
distribution, Sikkim Patterns of Diversity and Structure of
Oak Forests in Meghalaya along a
Disturbance Gradient
Genome Size Estimation of
Aabid Hussain Mir1* and Krishna Upadhaya2
Castanopsis Species (Fagaceae) from 1
Department of Environmental Studies, North-Eastern Hill
Meghalaya, India using Flow University, Shillong-793022, Meghalaya, INDIA;
Cytometry 2
Department of Basic Sciences and Social Sciences, North-
Eastern Hill University, Shillong-793022, Meghalaya, INDIA,
Kiran*, Babita Joshi, Gopal Ji Tiwari, Satya Email: aabidm4@gmail.com, upkri@yahoo.com
Narayan Jena and Saroj K. Barik Oak dominated forests represent the subtropical
Plant Molecular Genetics Laboratory, CSIR-National broadleaved vegetation of Meghalaya and are closely
Botanical Research Institute, Rana Pratap Marg, Lucknow- linked with the livelihood of rural people. They are used
226001, INDIA, Email: kiranbhasker691992@gmail.com for timber, fuelwood and for edible nuts. Oak species
also play a major role in the maintenance of ecosystem
Castanopsis, commonly known as chinkapin is
stability, soil fertility, watershed, and local biodiversity.
one of the dominant genera of Fagaceae. In addition
The current study highlights the diversity and structural
to the timber value, the raw acorns of most species of
patterns of Oak forests of Meghalaya. Fifteen forests,
the genus are eaten by human and wildlife indicating
along a disturbance gradient (low, medium and high)
ecological and economic importance of the genus.
were sampled during the study. A total of 258 woody
Genome size determination is a pre-requsite for
(e”5 cm dbh), 217 shrubs and 210 herbaceous species
calculation of covergae of any de novo sequencing.
were recorded. Tree species richness of the forests
Many studies have shown that genome size is an
varied from 34-75, density ranged from 560-1164
important indicator for distinguishing different taxa.
individuals ha-1, and stand basal area from 7.4-77.4
There are various techniques for assessing genome
m2ha-1 . Shrub and herb species density varied from
size i.e. 2C DNA content. Among all these, flow
2912-1008 and 109200-378000 individuals ha -1
cytometry (FCM) is convenient, rapid and precise for
respectively. A total of 11 species of Fagaceae viz.
detecting small variation in genome size. In the present
Quercus kurzii Hance, Q. armata Roxb., Q. indica
study, the leaf samples of three species of Castanopsis
(Roxb. ex Lindl.) Drake, Q. brunnea H. Lév., Q.
viz., C. purpuella, C. tribuloides and C. armata were
tribuloides Sm., Q. dealbata Hk. f. & Th. ex Miq.,
collected from Jarain, Sohrarim and Umsining of
Q. elegans Bl., Q. glauca Thunb., Q. griffithii Hk. f.
Meghalaya, respectively, and were analyzed for their
& Th. ex Miq., Q. kamroopii D. Don and Q.
2C DNA content with the help of flow cytometer. The
semiserrata Roxb were recorded. These species
results revealed that the genome size of three species
contributed 28% and 48% to the overall density and

126 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

stand basal area of the forests, respectively. that have been high-graded limit choices between
Disturbance favoured the growth of Fagaceae clearfell and regenerate naturally or underplant with
members as evident by a positive correlation with Quercus seedlings to alter composition. In this
density except for Q. glauca, which showed a reverse presentation, we will review current restoration methods
trend. Density-diameter distribution of the species including innovations from improvements in planting
showed the reverse J shaped curve, while basal area stock and new silvicultural systems that restore
showed an irregular pattern. Forests exposed to low ecological function more quickly than traditional
disturbances had a high density and basal area in large afforestation with single-species stands. We also
diameter (>55 cm) class. Based on the density of consider the challenge of managing the understocked
seedling, saplings and adult individuals, the regeneration restoration stands that have resulted from current
of the oak species may be rated as good in disturbed federal policy and incentive programs. The yet
habitats, except Q. glauca, which prefer to grow in unaddressed challenges to sustainable management that
undisturbed forests. Most of the Fagaceae members would come from altered climate and sea level rise
were heliophytic in nature, hence can be used in have also been discussed.
ecological restoration programmes. Key words: Nuttall oak, cherrybark oak, bottomland
Key words: Community forests, Fagaceae, Subtropical hardwoods, functional restoration, Wetlands Reserve
broadleaf forests, Traditional forest management Program, climate change

SV/P-5 SV/P-6

Restoring and Managing Quercus in Flow of Provisioning Services from

Riparian Forests of the Southern USA Oak Dominated Forest of Western
1 2
John A. Stanturf *, Emile S. Gardiner , Daniel C.
Dey3, Douglass F. Jacobs4 and Brian R. Lockhart5 Subhasis Mahato1 *, Sabyasachi Dasgupta 1,2 and
1 2
Estonian University of Life Sciences, Tartu, Estonia; US N.P. Todaria1
Forest Service, Stoneville Mississippi, USA; 3 US Forest 1
Department of Forestry and NR, H.N.B. Garhwal University,
Service, Columbia Missouri, USA; 4 Purdue University, West Srinagar (Garhwal), Uttarakhand-246174, INDIA;
Lafayette Indiana, USA; 5 US Forest Service, Stoneville 2
Department of Forestry and Biodiversity, Tripura University,
Mississippi, USA, Email: drdirt48@gmail.com Agartala, Tripura-799022, INDIA
More than 60 broadleaved species are endemic Stock of provisioning services from Western
to the riparian or floodplain forests in the southern USA. Himalaya has declined significantly during the last few
Diverse, mixed-species forests covered 10 million ha decades. Oak species assume considerable
in the Lower Mississippi Alluvial Valley (LMAV) in conservation significance in the region as they are the
the south–central USA but conversion to agriculture providers of numerous services and serve as a lifeline
has reduced the original forest cover to only 26% of its for the local population. This study explored forest
historic extent. Efforts over the last 30 years to restore resource-use patterns and villagers’ dependency on
these forests have concentrated on 10 native Quercus Government managed oak forest sites. Fuel and fodder
species because of their value for timber and mast biomass stock of 74.18t ha -1 was estimated in the
production for wildlife. Because these species are reserved oak forest. The estimated flow of fuel wood
difficult to regenerate naturally, planting bareroot and fodder were 4.23 t ha-1 & 4.62 t ha-1 respectively.
seedlings has been the primary means of afforestation. Per day fuel wood consumption was estimated at
Complex inundation regimes and other site factors, in 13.09±5.11 kg / family. Oak leaf fodder available in
addition to variation in species adaptations to flooding, the villages constitutes the major fodder for livestock
light, and soil conditions, have presented obstacles to throughout the year. Average fodder consumption per
successful restoration. In addition to afforestation on household per day was 32.31 kg. The biomass flow
former agricultural land, regeneration of remnant forests was estimated to be 8.86 t /ha year -1 . Of the total

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

accessible fuelwood and fodder biomass, only 11.9 % changing social dynamics this unregulated flow would
was extracted annually from the reserved forests. lead to unsustainable harvesting.
Though the current level of extraction from the reserved Key words: Beneficiaries, Oak forest, Flow of provisioning
forest seems to be sustainable in short term, with the services, Himalaya.

128 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Session VI
Biomonitoring and Bioremediation

Abstracts 27-30 November, 2018, NBRI, Lucknow 129

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

130 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVI/KN-1 Glutathione (GSH) plays important roles in

protecting cells from oxidative stress caused by
Phytoremediation of Arsenic- exposure to toxic metals, xenobiotics and other abiotic
Contaminated Soils: Molecular stresses. The g-glutamyl cycle maintains GHS
homeostasis, which involves GSH synthesis and
Mechanisms and Field Application degradation, and the recycling of the component amino
acids. Overexpression of GSH biosynthesis pathway
Lena Q. Ma1,2 genes increases the levels of GSH, which chelates with
College of Ecology, Southwest Forestry University, CHINA; toxic metals and hence provides tolerance in the plant
Soil and Water Science Department, University of Florida, tissues. Previously, we developed a genetics-based As
USA, Email: lqma@ufl.edu remediation strategy in Arabidopsis by tissue-specific
Arsenic is of great environmental concern due to expression of bacterial arsenate reductase, ArsC,
its extensive contamination and carcinogenic toxicity. gamma-glutamylcysteine synthase, g-ECS, genes and
Past human activities have resulted in many arsenic RNAi knockdown of an endogenous arsenate
contaminated sites worldwide. Our group reported the reductase AtACR2, which resulted in the efficient
first-known arsenic hyperaccumulator Chinese Brake extraction and hyperaccumulation of As into the above
fern (Pteris vittata) in Nature (2001). The plant has ground plant tissues. We have transferred this
the potential to phytoremediate arsenic-contaminated technology from Arabidopsis to high biomass, fast
soils. This presentation tries to show the molecular growing non-food crop- Crambe abyssinica for the
mechanisms behind the efficient arsenic uptake and application of this strategy to the As contaminated fields.
detoxification by P. vittata and optimization of its Further, we have identified a small gene family encoding
arsenic hyperaccumulation for phytoremediation ã-glutamyl cyclotransferases, GGCTs (GGCT1,
application in contaminated soils. Towards this goal, GGCT2;1 and GGCT2;2) in Arabidopsis, which are
we explored the roles of phosphate transporter and involved in the g-glutamyl cycle required for maintaining
ACR3 arsenite effluxer in arsenic uptake and efflux, GSH homeostasis via recycling Glu, a key nitrogen-
and the role of phytate in solubilizing arsenic and storing amino acid. The recombinant GGCT2;1 protein
phosphate from soils. In addition, we demonstrated the was able to convert ã-glutamyl-Ala and GSH to 5-
efficiency of P. vittata in remediating arsenic- oxoproline (5-OP), a precursor of glutamate (Glu) in
contaminated soils over 5 years. Therefore, this vitro conditions. Manipulation of GGCT genes in
presentation focuses not only on the mechanisms as Arabidopsis showed enhanced tolerance to multiple
well as application of P. vittata in phytoremediation. abiotic stresses, produced higher levels of Glu precursor
5-oxoproline (5-OP) as a result of GSH degradation.
Key words: Carcinogenic, Hyperaccumulator,
GGCT2;1 T-DNA mutants become severely sensitive
Phytoremediation, Phosphate transporter, Arsenite effluxer.
to ABA, salt, and mannitol. Our studies suggest that
SVI/KN-2 by efficient recycling of Glu as part of the g-glutamyl
cycle, GGCT2;1 decreased the de novo synthesis of
Glutathione Homeostasis Plays Glu, thereby decreasing the nitrogen requirement.
Therefore, GSH homeostasis plays important roles in
Critical Role in Protecting Plants from maintaining cellular redox for protection of plants under
Abiotic Stresses and in Heavy Metals abiotic stresses and holds great potential for developing
Phytoremediation ‘Climate Resilient Crops’ as well as developing plants
for phytoremediation of toxic metals and metalloids.
Om Parkash Dhankher*, Gurpal Singh, Ahmed G. Key words: G-glutamyl cycle, GSH synthesis, Glutamate
Meselhy and Bibin Paulose* recycling, Oxidative stress.
Stockbridge School of Agriculture, University of
Massachusetts, Amherst, MA, USA, Email: parkash@

Abstracts 27-30 November, 2018, NBRI, Lucknow 131

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVI/L-1 Deen Dayal Upadyay Gorakhpur University, Gorakhpur,
INDIA; 2CSIR-National Botanical Research Institute,
Economic Valuation of Ecosystem Lucknow, INDIA; 3UFZ-Helmholtz Centre for Environmental
Research, Leipzig, GERMANY; 4Universität Konstanz,
Ecological Services GERMANY; 5Deutsches Elektronen-Synchrotron (DESY),
Hamburg, GERMANY, Email: seema_mishra2003
Mir Mehrdad Mirsanjari @yahoo.co.in
Department of Environmental Sciences, Faculty of Natural Toxic metals and metalloids not only restrict plant
Resources and Environment, Malayer University, Malayer, growth and productivity, but also cause food chain
IRAN, Email: mmmirsanjari@malayeru.ac.ir contamination resulting in risk to human health. One of
Each ecosystem is a dynamic set of variables, the most relevant examples is the contamination of
each of which plays an essential role in the formation ground water with arsenic (As) in the Indian
of an ecosystem. In order to determine the economic subcontinent. It affects health of millions through
value of each ecosystem, it is necessary to pay attention drinking water and food chain contamination and
to all variables and their role, otherwise the value stated threatens the sustainability of food production.
does not indicate the true value of the ecosystem and Understanding the mechanism of As toxicity is crucial
in the long run, due to miscalculations and less economic for finding a sustainable solution to the problem, and
value, the space to destroy the creation It turns out.The determining the in planta distribution and speciation of
United Nations, the IMF, the World Bank, and the As are important steps in this process. Speciation and
Organization for Economic Co-operation and compartmentation of arsenic (As) in Ceratophyllum
Development (ICECD) have developed a “integrated demersum, a good laboratory model for shoot, and rice,
accounting system for the environment and economy”, the most As affected crop, was performed to explore
based on the results of previous studies on the mechanism of As toxicity. Speciation was analyzed
environmental asset valuation. In the recipe, chromatographically (high performance liquid
environmental assets consist of ecosystem goods and chromatography-[inductively coupled plasma-mass
services.Valuation, the ecosystem of two aspects, one spectrometry]-[electrospray ionization-mass
of the values of use and the other non-usable values spectrometry]) in whole-plant extracts and by tissue-
will be examined. Usage values are divided into two resolution confocal µ-X-ray absorption near-edge
groups: consumable (such as wood products) and non- spectroscopy (ì-XANES) in intact shock-frozen
consumable. Non-consumable values include direct hydrated root and leaves. The sub-cellular element
values (such as outing) and indirect values (such as distribution (compartmentation) was analyzed through
the value of water, soil and gas conservation). The µ-X-ray fluorescence (ì-XRF).Speciation through
ecosystem’s non-use values can also be seen as the chromatography revealed that in C. demersum, up to
conservation value of forests and the value of wildlife 60% accumulated As was in the form of thiol
conservation.In this research, we intend to determine complexed-AsIII. Most of which was in epidermis of
the economic value of the ecosystem based on the mature leaves as revealed by tissue resolved ì-XANES
ecological services function. of intact hydrated leaves. At sublethal concentration,
Key words: Ecosystem, Economic value.
As predominantly accumulated in the nucleus of the
epidermal cells, as revealed by ì-XRF analysis,
SVI/O-1 indicating replacement of P by As in DNA molecules,
providing first in vivo evidence for arsenic induced
Speciation and Compartmentation of DNA damage. While at lethal concentration, vacuole
was the main storage site of As, yet a significant
Arsenic in Plants and Mechanism of increase of unbound AsIII in mesophylls of young
its Sub-Lethal Toxicity mature leaves occurred. This small amount of As
reaching chloroplasts caused a strong and specific
Seema Mishra1,2,3,4 , Sanjay Dwivedi 1 , Rudra D. inhibition of tetrapyrrole biosynthesis and severe growth
Tripathi1, Gerald Falkenberg5, Jürgen Mattusch3 retardation. In rice plant, exposed to inorganic arsenate
and Hendrik Küpper4

132 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

(AsV) and methylated As, most of the AsV and was proven to be one of the important heavy metals
methylarsonate (MAV) were efficiently reduced to emissions sources in the present day urban scenario.
arsenite (AsIII) and MAIII respectively, but Using biological markers can potentially improve the
dimethylarsinate (DMAV) did not transformed. A large way in which exposure to environmental factors is
proportion of AsIII and MAIII were complexed with assessed. At present, however, only a few valid
thiols showing up to 20 and 16 As species respectively biological markers are available that can be effectively
in the roots. Many of them were identified as new As- used in epidemiological studies. The use of human hair
thiol species. Despite high complexation in root, more analysis technique can be used as a tool for monitoring
MA was translocated to shoot, with shoot/root transfer exposures to environmental pollutants. Of late, this
factor being in order DMA>MA>inorganic As in rice. technique has proved to be a well-suited biological
Further, the distribution study of As in frozen hydrated marker of occupational and environmental exposure
roots (both the root tip and the root hair zone) of rice of man. During the past three decades the
revealed that in the main root, As was mostly confined determination of trace elements levels in hair has been
to the endodermis. Whereas, in the young root branches the subject of continual interest in the biomedical and
apart from endodermis arsenic was also spread into environmental sciences (Arnold 1994 and Ciszewski
the cortex, particularly in younger, not fully 1997). In the present study concentration of four toxic
differentiated tissue including root tip. This shows that metals Pb, Cu, Cr and Zn was determined, using
As is taken up inadvertently by young tissues without standard methods, in human scalp hair residing near
proper control. In conclusion at a concentration that heavy traffic areas of Ajmer city (Rajasthan). Sample
can be easily attained in shoots of crop plants, As is were collected, decontaminated and digested before
already toxic to plants, i.e.it can inhibit pigment analysis using Atomic Absorption Spectrophotometer.
biosynthesis and cause DNA damage leading to The aim of the present study was to determine the
hampered growth and reported yield loss. This concentration of the chosen metals in hair of subjects
knowledge can be a starting point for targeted breeding residing near the sites of study and assess the
of more resistant plants. comparative burden of vehicular pollution in the selected
Key words: Arsenic, Ceratophyllum demersum, areas.
Compartmentation of arsenic, Rice, Speciation of arsenic, ì- Key words: Biomarkers, Vehicular pollution, Heavy metals,
XANES, ì-XRF. Scalp hairs.


A Study of Heavy Metal Pollution from Bioremediation Potential of

Motor Vehicle Emissions using Human Macrophytes from Jakkur Wetland,
Hair as Biomarkers Bangalore
Krishna Mishra P. Sudarshan1,2*, Mahesh M.K.1 and Ramachandra
Samrat Prithviraj Chouhan Government College, Ajmer, T.V.2
Rajasthan, INDIA, Email: drkrishnamishra@gmail.com 1
Department of Botany, Yuvaraja’s College, Mysore-570005,
The fuel combustion by the vehicles on the road Karnataka, INDIA; 2Energy and Wetlands Research Group,
Centre for Ecological Sciences, Indian Institute of Science,
emit complex mixtures of air pollutants, including heavy
Bangalore-560012, Karnataka, INDIA, Email: bhat.
metals like Cd, Cu, Cr, Ni, Pb and Zn. Of all of these sudarshanp@gmail.com; mkmahesh44@yahoo.com,
pollutants, fine particulate matter has the greatest effect tvr@iisc.ac.in
on human health. Heavy metals represent a class of
omnipresent pollutants, with toxic potential, in some Macrophytes are important components of lake
cases even at low exposure levels. In most of the cases, ecosystem. They have the ability to remove organic,
heavy metals pollution problem is associated with inorganic nutrients and heavy metals from environment
intense industrialization. However, high vehicle traffic and act as bioremediators. The present study was done

Abstracts 27-30 November, 2018, NBRI, Lucknow 133

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

to assess biomass, nutrient and metal accumulation SVI/O-4

ability of macrophytes from Jakkur Lake, Bangalore
and use it as bioindicator. Macrophytesand water Two-Pronged Approach to Ascertain
samples were taken from inlet and outlet of the lake. Cadmium Phytoremediation Ability of
After identifying species, they were washed thoroughly
to remove adhering sediments and periphytons. They Pistia stratiotes: Biochemical and
were dried to constant weight using hot air oven and SEM-EDX Analysis along with Catfish
the dry weight was determined. The dried samples were Bioassay using Clarias batrachus
powdered to get fine powders and were analysed for
nutrient and heavy metals using standard procedures. Sunayana Goswami1 and Suchismita Das2 *
Polygonum glabrum and Typha angustata had higher
biomass in inlet and outlet respectively. In inlet Centre for Soft Matter, Department of Chemistry, Assam
University, Silchar-788011, INDIA; 2Aquatic Toxicology and
Polygonum, Alternanthera philoxeroides and Typha
Remediation Laboratory, Department of Life Science and
angustata had higher concentration of Carbon,
Bioinformatics, Assam University, Silchar-788011, INDIA,
Nitrogen and Phosphorus respectively. Carbon content Email: drsuchismita9@gmail.com
was higher in Typha angustata, Nitrogen and
Phosphorus was highest in Eichhornia crassipes in Aquatic macrophytes have tremendous potential
the outlet of the Lake. Cadmium concentration was for remediation of the heavy metal cadmium (Cd) and
within normal range with Alternanthera philoxeroides can be used as an alternative way of reducing aquatic
and Pistia stratiotes accumulating highest in inlet and metal burden. Although studies on phytoremediation
outlet. Typha angustata in inlet had Copper are abundant, often there is a lack of validation of the
concentration in critical range whereas in outlet all process by suitable bioassay. Thus, we investigated the
species had normal range. Lead, Zinc, Nickel and remediation potential of Pistia stratiotes exposed to 5,
Chromium was in higher concentration in Typha 10, 15 and 20 mg Cd L-1 in 0.1 × Hoagland solution for
angustata than other species in inlet. Nickel was above 21 d in hydroponics and estimated the efficacy of this
normal range in Typha angustata in inlet and in all endeavour with catfish bioassay. The uptake maxima
species in outlet. In the outlet Typha angustata (Lead), of 11670 µg Cd g-1 dry wt. in root and 5750 µg Cd g-1
Eichhornia crassipes (Zinc and Nickel) and dry wt. in shoot of Pistia was observed. Antioxidant
Alternanthera philoxeroides (Chromium) had higher enzyme activities, such as, superoxide dismutase,
concentrations. The heavy metals in the water samples peroxidise, catalase and ascorbylperoxidase were
were as follows Zn>Cu>Pb>Cr>Cd>Ni. The estimated to ascertain stress tolerances. Our findings
bioconcentration factor was in the following order support that the bioaccumulation of Cd in P. stratoites
Cd>Cr>Ni>Pb>Cu>Zn. Thus the study highlighted the was accompanied by augmentation of cellular
bioremediation potential of Macrophytes from Jakkur antioxidants enzyme activities to protect itself against
Lake. Cd-induced toxicity. Cd induced alterations in
morphology, growth, as well as the localization of Cd
Key words: Macrophytes, Bengaluru, Jakkur Lake, Heavy with the Scanning electron microscopic (SEM) coupled
metal, Bioremediation.
with energy-dispersive X-ray microanalysis (EDX) on
P. stratiotes root and leaf were estimated. For catfish
bioassay with Clarias batrachus, 5 and 10 mg Cd L-
Pistia remediated water was used to rear the catfish
for 7 days after which Cd uptake in various tissues of
fish, as well as analysis of biochemical responses, and
antioxidant enzyme profiling of the gill and liver tissues
were performed. Fish muscle accumulated 28.8-39.8
ìg Cd g-1 dry wt. when reared in Cd only doses,
however, in fish reared in Pistia remediated water,
muscle accumulated 9.2-14.2 ìg Cd g-1 dry wt. which

134 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

was within the safe levels of Cd in edible tissues. The (phytolith) within and around the cells by grasses has
results pointed towards the efficacy of Cd emerged as a feature of tremendous potential in
phytoextration by P. stratiotes and suitability of this environmental monitoring and mitigation. As phytoliths
plant for the large scale remediation purposes. show variations in size in relation to changes in the
Key words: Cadmium, Phytoremediation, Antioxidants, ambient growth temperatures, they have proved to be
Uptake, Catfish bioassay. ideal markers for monitoring global warming and climate
change. As phytoliths occlude organic carbon of the
SVI/O-5 cytoplasm and stay unchanged in the soil for millenia,
they have tremendous potential in carbon sequestration.
Role of Grasses in Monitoring Global Also, persistence of biogenic silica in the soil converts
Warming and its Mitigation through the simple geochemical weathering of silica into a
biogeochemical cycle within which operates the shorter
Carbon Sequestration biological silica cycle. Owing to excellent preservation
down the millenia and diagnostic variations in
Amarjit Singh Soodan morphotypes, phytoliths have found an ever-increasing
Department of Botanical and Environmental Sciences, Guru role in understanding palaeoecology and
Nanak Dev University, Amritsar-143005, Punjab, INDIA, palaeoclimatology of the planet besides playing a role
Email: assoodan@gmail.com in contemporary environmental mitigation. The
The uncontrolled burning of fossil fuels has lead dynamics of phytolith accumulation in grasses with
to tremendous increase in green house gases (GHS’s), special emphasis on their role in carbon sequestration
mainly carbon dioxide and the resultant global warming shall be discussed in the lecture.
and its harmful effects. Scientists are looking for model Key words: Carbon sequestration, Environmental monitors,
organisms that could help monitor the pace, quantum Grasses, Palaeoecology, Phenology, Phytoliths.
and direction of climate changes taking place at regional,
national and global levels as also to reduce the SVI/O-6
concentration of GHG’s in the atmosphere and mitigate
their harmful effects. Grasses meet all the requirements Rhizo-Remediation of Hazardous
of environmental monitors including a cosmopolitan Waste: A Sustainable Approach for
distribution, high species diversity, habitat specificity
Environment Clean-up
and climatic zonation from tropical through subtropical,
temperate, alpine and the polar regions. Grasses display Bhawana Pathak
a closely coordinated cycle of well defined phenophases
in the vegetative and reproductive phenology that could School of Environment and Sustainable Development,
be utilized for assessing the impact of climatic factors Central University of Gujarat, Gandhinagar-382030, INDIA,
Email: bhawana.pathak@cug.ac.in
such as temperature, precipitation and duration of
sunshine. Grasses show special leaf anatomy correlated Industrial and technological revolution is an
with carbon assimilation cycles in cold and dry (C3) economic indicator for consideration as the principle
and hot and humid (C4) conditions. India is home to criteria for measuring progress. Technological
several cultivated (cereals and millets) and wild grasses revolution has lead to changes in the industrial product
that dominate the cultivated fields and vast ecosystems and processes. The development of product and
(grasslands) of the country. One only needs to earmark processes in the industries has generated wastes;
permanent environmental monitoring plots in natural enhanced environmental pollution and cause threat to
grasslands in different climate zones and select grass human life. The present treatment technologies -
species for experimental observations on phenological laboratory scale are insufficient to treat pollutants to
changes. Some such grass species have been identified acceptable level (contaminated land). Treatment
during our investigations in the North-West Himalayan technologies also resulted in the release of large number
region. Apart from possessing a well defined phenology, of natural and synthetic hazardous compounds (organic
accumulation of silica in the form of silica bodies and inorganic chemicals) due to lack of effective waste

Abstracts 27-30 November, 2018, NBRI, Lucknow 135

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

treatment especially in industries like chemical, dyes, The arsenic contamination in soil and water has
textile, printing, construction and metal manufacturing increased up to levels that are unsafe for humans as
sectors. Considering all these flaws of physicochemical well as plants and animals. The problem of arsenic
treatment processes for remediation of hazardous contamination in ground water is severe in Bangladesh
compounds present in the industrial effluent, and in the state West Bengal of India. The release of
biotechnological approaches i.e bioremediation and industrial wastes, sewage waste and solid waste
phytoremediation are the emerging areas to remediate dumping has alleviated the problem of water
the environmental contaminants and are cost effective, contamination. Phytoremediation has been the cheap
eco-friendly and produce less toxic compounds and an and environmental friendly method to reduce
alternative method offers the possibility to destroy contamination, and thus, a hydroponic experiment with
harmful contaminants with the help of natural biological Phragmittes australis (Ph) and Vetiveria zizanioides
activity. Rhizoremediation is the superior biodegradation (Vt) was designed. The study was carried out using
of organic pollutants/ hazardous waste by root- different densities of accumulator (12 and 24 Ph, and
associated bacteria and fungi under the influence of Vt) in trays with arsenic concentration of 1 ppm, and
plant species. The use of plants and native remediation was observed with time duration (20 days).
microorganisms to degrade or remove pollutants has Sampling of water was done after every 2 days. It
established as an effective technology for in situ was found that arsenic concentration in water
remediation. The potential role of plants and associated decreased with time duration and density of the
rhizomicrobial population for in situ bioremediation of accumulators. Complete remediation of arsenic was
hazardous organic compounds is significant. The achieved after 20 days with 24 Ph and 24 Vt, and the
present paper focuses on the rhizoremediation of dye arsenic remaining in the medium was 0.04 and 0.25
compound and pesticides as case studies. The different ppb respectively. With these observations a 4 stage
factors influencing the plant-microbe-toxicant setup was made with 24 Ph and 24 Vt with same
interaction in soils is also highlighted. Rhizoremediation concentration of arsenic and sampling of water after
is a promising technology for the removal of different every 5 days. The arsenic concentration in water after
hazardous compounds from soil, and the developed 20 days was reduced, and was within the safe limit set
technology in the laboratory is helpful for transfer of by WHO with both accumulators. The 4 stage setup
technology from lab to land - a green technology for can be applied to arsenic contaminated irrigation water,
sustainable environment. reducing the further contamination of soil and toxic
Key words: Environmental pollution, Hazardous waste, effects on plants.
Treatment technology, Rhizoremediation, Technology Key words: Arsenic, Hydroponic, Phragmittes australis,
transfer - lab to land. Vetiveria zizanioides, 4 stage setup.


A 4 Stage Setup for Phytoremediation Physiological and Biochemical

of Arsenic Contaminated Water with Assessment of Five Varieties of Helia-
Phragmittes australis and Vetiveria nthus annuus for Phytoextraction of Lead
Jyoti Mathur
1,2 1
Ashish Praveen and Nandita Singh Department of Bioscience and Biotechnology, Banasthali
Plant Ecology and Environmental Science Division, National University, Banasthali Rajasthan, INDIA, Email:
Botanical Research Institute, Lucknow-226001, INDIA; jyotijnp21@yahoo.co.in
Academy of Scientific and Innovative Research (AcSIR), Heavy metal contamination in the soil as well as
Plant Ecology and Environmental Science Division, National water is becoming an irresolvable problem. Lead (Pb)
Botanical Research Institute, Lucknow-226001, INDIA, is one of the most toxic heavy metal contaminant in
Email: aashishpraveen45@gmail.com, nanditasingh8
the environment. Pb has no specific biological function

136 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

but can cause morphological, physiological and Telangana state was formed on 2nd June, 2014 and
biochemical dysfunctions in plants, animals and humans. project opening date was on 4th August 2016. It is
Phytoextraction is one of the possible method for situated in Yellampalli village, Ramagundam, Mancherial
mitigation of heavy metals from the environment. District (old Adilabad District) in Telangana State, India.
Helianthus annuus has been identified as a good metal The project is fourth largest one on Godavari river in
hyperaccumulating plant. Therefore, the present study Telangana. The project would supply water for 6tmc
has been carried out for screening of lead to NTPC Ramagundam city and Hyderabad City. After
hyperaccumulation potential of five varieties of H. the flood gates are installed, the project would store
annuus (DRSF-108, DRSF-113, Phule Bhaskar, about 20tmc of water. Barrage type of Dam height
LSFH-171 and KBSH-44). Seedlings of sunflower 263m, length 1180.7m, spillways 62. Construction cost
were treated with different concentrations (0, 50, 100, 5400 crore rupees. Its geographical coordinates
300, 500, 700 and 1000 ppm) of Pb(NO3)2. Plantlets 18050’45" North and 79022’05" East.
were harvested at 20, 40 and 60 days of time intervals.
The water samples were collected at 9 stations
Physiological studies such as root length and shoot
for analysis of physico-chemical and biological
length, fresh and dry weight of shoot and root of plants
parameters in three different seasons (rainy, winter,
performed. Concentration of Pb in various plant parts
summer) during 2017-18. Physical parameters like
were estimated by atomic absorption
appearance, color, odor, temperature, electrical
spectrophotometer. This study showed maximum
conductivity, turbidity, etc.; chemically characteristics
accumulation (693.69 ppm) of Pb in Phule Bhaskar
such as dissolved oxygen, biochemical oxygen demand,
and minimum accumulation (333.16 ppm) in KBSH-
chemical oxygen demand, total alkalinity, total hardness,
44 at 1000 ppm after 60 days. Phule Bhaskar
calcium, magnesium, chloride, free carbon-dioxide,
accumulated maximum (394.32 ppm) Pb in the roots.
phosphates were analyzed. Biological parameters like
Level of proline and polyphenol increased with
algae, fungal, bacterial, actinomycetes population were
increasing Pb concentration was reported in treated
analyzed and compared with IBS (10800) 2012. The
leaves of all varieties. The maximum proline and
total values indicate that river water belongs to the
polyphenol contents were 31.16 µmol g-1 and 7.15 mg
category of excellence to good water quality as per
g-1 respectively at 60 days. Thus, the results from this
IBS (10800) 2012.
study showed that among the five varieties, Phule
Bhaskar had a greater survivability and ability for lead Key words: Physical, Chemical, Biological parameter, BOD,
accumulation than rest of other varieties proving as COD.
good hyperaccumulating variety.
Key words: Helianthus annuus, Lead, Phytoextraction,
Heavy metals. Characterization of Iron Infused
SVI/O-9 Activated Carbon Developed from
Citrus limetta Biomass: An Application
Physico-Chemical and Biological for Theremoval of Fluoride from
Characteristics of Godavari River Aqueous Solution
Water at Sripada Yellampalli Project,
Mancherial Dist., Telangana, India Lata Verma*, Mohd. Ibrahim and Jiwan Singh
Department of Environmental Science, Babasaheb Bhimrao
B. Lalitha Kumari Ambedkar University, Lucknow-226025, INDIA, Email:
Department of Botany, Kakatiya University, Warangal, lataverma2501@gmail.com, mohdibra1144@gmail.com,
Telangana, INDIA, Email: lalitha21prasad@gmail.com jiwansingh95@gmail.com

Sripada Yellampalli Project foundation was laid In this study, a low cost adsorbent was prepared
on 28th July, 2004. It started in 2005, Andhra Pradesh. at 500oC temperatures from pulp of Citrus limetta and

Abstracts 27-30 November, 2018, NBRI, Lucknow 137

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

applied for the treatment of fluoride contaminated around Kanpur from two points (middle of the river
water. Prepared bioadsorbent was characterized stream and a discharge point) at each location. Metals:
through X-ray diffraction, Fourier transform infrared Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Mo, Cd, Se and Pb
spectroscopy, pHzpc and scanning electron microscopy. were detected in all the samples. The metals showed
This bioadsorbent shows very effective removal of no noteworthy disparity with respect to sampling
fluoride as observed in batch adsorption study which locations as well as discharge points. However, the
was performed at different temperature (298K, 308K, concentration of the metals varied with season, being
318K and 328K), pH (4-10), dose (0.5, 1.0, 2.0, and higher in summer and lower in rainy season. Out of all
3.0 g L-1) and concentrations (1-30 mg L-1). The result the metals found in the study site, Cr, Fe Cu, As and
shows maximum removal of 86% with ACP-500. Pb concentration in all the sample during each season
Adsorption isotherm, intraparticle diffusion, at all the study sites are higher than the prescribed
thermodynamics and kinetics reaction were also limit by Bureau of Indian Standard (BIS) and World
studied. For kinetic study, pseudo-second-order kinetics Health Organization (WHO) for drinking, aquatic life
was best fitted and in adsorption study it was observed and irrigation. Result of pollution indices shows that
that Langmuir isotherm model well fitted. Adsorption river water is not suitable for drinking and aquatic life
process was found to be exothermic, it represents but suitable for irrigation purpose only.
removal percentage decreased with increasing Key words: Metals, Seasonal variation, River Ganga,
temperature. Regeneration study revealed that the Pollution indices.
bioadsorbent can be reused three to four times. The
authors are grateful to the SERB-DST, Government SVI/O-12
of India (Project No. ECR/2016/001924) for providing
financial support for this work. Role of Bacteria in Remediation of
Key words: Adsorption, Isotherm, Kinetics, Fluoride, Indoor Pollution with the Help of
Aglaonema commutatum Schott
Seemaa Ghate
Monitoring and Assessment of Know How Foundation, C-602, Vaidehi Enclave, Bavdhan,
Pollution Load in Terms of Heavy Pune, Maharashtra, INDIA, Email: drseemaaghate@
Metals in Surface Water of River
Role of Sphingomonas, gram negative bacteria
Ganga around Kanpur: A Study for was examined in remediation of Indoor pollution with
Suitability of this Water for Different help of Aglaonema commutatum. Sphingomonas was
isolated and inoculated in the medium in which
Utilities experimental plants were grown. Aglaonema was
tested against pollutants released from household
Vandana Singh,* Nem Kumar Nagpoore, Jai
products like mosquito coil and incense sticks. It was
Chand and Alok Lehri
found that Sphinomonas helped plant in the activity of
Central Instrumentation Facility, National Botanical Research phytoremediation. Exposure to Incense sticks showed
Institute, Rana Pratap Marg, Lucknow-226001, INDIA, Email: no absorption of any pollutant under consideration.
singh_vini02@yahoo.in Exposure to smoke from mosquito coil showed that
A study was conducted during April, 2016–March, levels of allethrins absorbed by plant, were very high,
2017 to evaluate the surface water quality of river up to 20-30 ppb as tested on GCMS. When GCMS
Ganga around Kanpur. The samples were analyzed analysis of same plant was done after 20 days it was
for a number of physico-chemical parameters using observed that levels of allethrin reduced to 0. Allethrins
standard laboratory procedures and giving prime plunge may be digested by Aglaonema plant with help of
to determine the heavy metal concentrations of surface Sphingomonas, as this bacteria helps phytoremediation
water at five different locations of the river Ganga activity. Further research on this line is in the channel.

138 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Key words: Sphingomonas, Aglaonema, Indoor pollution, strongly suggest that greater phytoextraction can be
Mosquito coil, Incense sticks, Phytoremediation. accomplished by the application of environment friendly
and biodegradable chelant NTA.
Key words: NTA, Chelant, Phytoextraction,
Phytoextraction of Lead from Soil Hyperaccumulator.

using Biodegradable Chelant SVI/O-14

(Nitrilotriacetic Acid) by Coronopus
Silicon: A Source of Mitigator to
Heavy Metal (Arsenic) Stress in Rice
Riya Raina1 *, Harminder Pal Singh1 and Daizy Grains
R. Batish2
Department of Environmental Studies, Panjab University, Vinod Goyal1*, Sanjay Dwivedi2, Seema Mishra2,3,
Chandigarh-160014, INDIA; 2Department of Botany, Panjab Neeru Jain4 and R.D. Tripathi2
University, Chandigarh-160014, INDIA, Email: 1
Department of Botany & Plant Physiology, CCS HAU, Hisar-
riyaraina997@gmail.com, hpsingh_01@pu.ac.in, 125001, INDIA; 2Plant Ecology and Environment Science
daizybatish@yahoo.com Division, CSIR-National Botanical Research Institute,
Lead (Pb) is one of the most persistent and Lucknow -226001, INDIA; DDU University, Gorakhpur,
widespread heavy metals of major environmental INDIA; 4Privi Life Sciences Pvt. Ltd., Mumbai, INDIA,
concern. Its contamination in soil represents a major
threat to human health. Chelate assisted Indian soils are mostly contaminated with heavy
phytoremediation has gained attention as a potential metals and among the heavy metals arsenic (As) is
alternative to traditional remediation methods because most common and toxic metalloid affecting the human
of lower cost and minimal soil disruption. In our study, health. It is continuously being added in the food chain
we have targeted the South American Native due to excessive use of fertilizers and changing of cation
(Coronopus didymus) because of its ability to produce exchange capacity of soils that cause its preferred
high biomass, good tolerance and accumulation of metals uptake by the plants. Present study is an attempt to
from soil. Nitrilotriacetic acid (NTA) has been used as study the interaction of silicon, a micronutrient and
a chelant to solubilize Pb and to enhance its uptake by uptake of arsenic/accumulations in rice grains. The
Coronopus didymus. Because of its short half-life of source of silicon used is the commercially available
2 to 7 days and fast degradation rate in soil, it does not ortho silicic acid (OSA), which is the only bioavailabe
pose any serious threat to the environment. Pot form. Application of arsenic in the form of arsenate
experiments were conducted for 4 to 12 weeks under and arsenite significantly reduced the growth of plants
completely randomized controlled conditions using 1000 in dose dependent manner. ArsenateV significantly
to 3000 mg kg-1 of Pbinsoil with or without NTA (4 reduced the crop yield, by affecting the yield and its
mMol). Growth, biomass, photosynthetic efficiency, lead attributes more than arsenite III. Independently
uptake and accumulation by the plant were compared. accumulation of arsenic was seen in rice grains, when
Results showed a good accumulation and tolerance of treated with different concentration of arsenic
Pb in the treated plants as compared to the controlled [arsenateV (25 and 50 mg l-1) and arseniteIII (20 and 25
plants thus, proving it, as a good hyperaccumulator. mg l-1)] along with other trace elements, where as when
Further, a comparison was done between the plants arsenic was applied along with silicon, the significant
treated with different concentrations of lead alone and reduction in arsenic accumulation in rice grains was
the plants treated with lead+NTA. Results have shown observed over control (No treatment/ arsenic alone).
that the addition of NTA has increased the uptake and Significantly lower accumulation of zinc and cobalt was
accumulation of Pb in roots and shoots of C. didymus, observed in grains of arsenic exposed plants, whereas
hence, it has a good efficacy in mobilizing and increased accumulation was observed for copper,
remediating Pb from the contaminated soils. The results manganese and selenium ions. It was observed

Abstracts 27-30 November, 2018, NBRI, Lucknow 139

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

application of silicon (soil/foliar), enhanced the growth results, physicochemical treatment combined with
and yield of rice plants by increasing the activity of biological treatment showed some sort of efficiency
antioxidants enzymes and simultaneously leading to rather than physicochemical treatment with
reduced accumulation of arsenic in grains. Parallel sophisticated techniques like electro coagulation with
studies revealed that foliar application of silicon is more chemical coagulation. However, it is very clear from
beneficial to the plants rather than soil based application. the study that industries have made significant efforts
The study concludes that the silicon application in the to comply with tolerance limits of industrial discharges
form OSA had a significant contribution in increasing to inland surface waters with high organic waste load.
the quality and quantity of the rice produce from the Further, it was evident that combined system of physical,
heavy metal affected areas. chemical and biological treatment facilities has shown
Key words: Rice, Arsenic, Silicon, Heavy metal stress. a remarkable efficiency in achieving the expected
SVI/O-15 Key words: Wastewater, Pollution, Industrial discharges,
Textile processing, Electro coagulation.
Assessment on Environmental Issues
Related to Water Pollution Associated SVI/O-16
with Textile Processing Industries Heavy Metal and Prospects of
Indehiwala-Mt. Lavinia and Moratuwa Phytoremediation in India
Municipal Council Areas in Sri Lanka
Jamshed Zaidi and Amit Pal*
1 2
M.R. Namalie and T.K. Weerasinghe Institute of Environment & Development Studies,
Central Environmental Authority, Denzil Kobbekaduwa Bundelkhand University, Jhansi-284128, INDIA, Email:
Mawatha, Battaramulla, SRI LANKA; 2Centre for apu13@rediffmail.com, jamshedphd@gmail.com
Environmental Studies and Sustainable Development, The Many fold increase of population and the pace of
Open University of Sri Lanka, Nawala, Nugegoda, SRI industrialization and urbanization have caused the
LANKA, Email:namalie44@gmail.com
widespread dissemination of heavy metals in our
The textile processing and garment industry is environment. Heavy metals are among the most
one of the most important industrial sectors in Sri Lanka. hazardous contaminants that may be present within the
This study mainly focused on assessment of aquatic ecosystem. They pose a serious threat to aquatic
environmental issues related to water pollution during biodiversity, and severe health risk for humans due to
textile processing while introducing appropriate the intake of contaminated water. Several existing
mitigatory measures. During the study, existing pollution technologies have been used to reduce aquatic
control measures to obtain Environmental Protection pollution, but they are generally not cost-effective, are
License from the Central Environmental Authority of labor-intensive and generate secondary waste. It is an
16 industries with different scales (large/medium and intriguing option which is an eco-friendly and affordable
small) were investigated. A detailed study was carried technique. Phytoremediation, which is growing to be
out in 07 industries including wastewater treatment the most attractive innovation in this area, utilizes plants
systems, volume of discharge or consumption of water, for the evacuation or alteration of ecological
work force, production capacity and mode of discharge contaminants, causing them to become innocuous. In
of treated waste water to the environment while this chapter, we summarize use of this technique, which
analyzing the treated effluent. The obtained results may play a strong role in the elimination of heavy metals
were statistically analyzed to find out suitable from our aquatic environment using some existing
relationships and possible significant differences. Seven macrophytes available in Bundelkhand region of Uttar
critical parameters such as temperature, colour, pH, Pradesh.
BOD, COD, TSS and oil and grease content were Key words: Heavy metal, Phytoremediation, Aquatic
measured for a period of six months. According to the macrophytes.

140 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVI/O-17 SVI/O-18

Assessment of Lukha River (Wah River Water Quality in Relation to

Lukha) with Emphasis on the Coloration Alluvial Deposition and Ecological
Phenomena during Winter Months Approach
Vivek Kumar Brajesh K. Dwivedi
Department of Environmental Studies, North-Eastern Hill Environmental Sciences, Botany Department, University of
University, Shillong-793022, INDIA, Email: naturevivek001 Allahabad, Allahabad-211002, INDIA
@gmail.com; naturevivek001@nehu.ac.in
Email: drbkdwivedi2@gmail.com, brajesh_env@ rediffmail
Lukha River (Wah Lukha) is one of the most .com
important rivers flowing in the Jaintia Hills region in Alluvial is a natural and essential component of
Meghalaya, India. It has been a source of livelihood river systems and plays a major role in the hydrological,
for the local people through the provision of fish geo-morphological and ecological functioning of rivers.
resources. In 2007, during winter the river turned sky- In many areas of the world, the level of anthropogenic
blue in colour and fishes were found dead and floating activity is such that fine-grained sediment fluxes have
on the water surface. Since then, every year the river been, or are being, modified at a magnitude and rate
turns turquoise to sky-blue during the winter months that cause profound, and sometimes irreversible,
and along with this phenomenon of color change, fishes changes in the way that river systems function. This
are found dead and floating on the water surface. paper examines how anthropogenic activity has caused
During the period that the river retains this turquoise to significant changes in the quantity and quality of
sky-blue coloration, no fish species have been sighted alluvials and their deposition/ influx in the river terrain
in the river. Effluents from coal and limestone mining of Ganga at whole stretch of Allahabad. Heavy metals
and also from the nearby cement industries have been namely Co, Cu, Cr, Ni, Cd, Zn, and Pb were examined
blamed for the change in color of the river, but no clear from the freshly deposited river bed sediment on a
explanation has emerged as yet. The present study seasonal basis for the assessment of two consecutive
focused on finding out the reason for turquoise to sky- years 2014-2015 and 2015-2016. The heavy metal
blue color of river during winter months, to examine concentration ranged between 09.37-26.12, 2.16-09.14,
water quality by testing physicochemical properties of 41.25-67.08, 13.29-23.59, 0.19-0.28, 11.26-15.59 and
river water, and also to check if any macro-aquatic life 08.71-11.26 mg kg-1 for Co, Cu, Cr, Cd, Ni, Zn and Pb,
is surviving in the colored stretch of river during respectively in different seasons. The highest
coloration period. The study revealed that the turquoise contamination degree of the sediment was noticed as
to sky-blue appearance of Lukha River is due to the 5.23. Geo-accumulation index was noted between (0
scattering of sunlight by the suspended aluminosilicate and 2, class 2) which showed that sediment was
particles in the river water. The study also revealed contaminated to moderately contaminated and may
that water quality of the river is deteriorated and is not have adverse affects on fresh water ecology of the
fit for domestic use. One glassy fish species was found river. Pollution load index (PLI) was found highest
surviving in a remote pocket of the colored stretch of (01.25), which indicates that the river alluvial/ sediment
the river. has a moderate level of contamination. The amounts
Key words: Water pollution, Lukha River, Aluminosilicate of nutrient were dynamically influenced with time
particles, Sky-blue river, Mining pollution, Meghalaya. periods, water availability and their flow.These running
water quality deterioration which may be due to
insufficient water availability, flow, alluvials deposition
and pollution. Various pollution indicators revealed that
the river bed sediment is less contaminated by toxic
metals during the study but the alluvials/ sediment

Abstracts 27-30 November, 2018, NBRI, Lucknow 141

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

quality may degrade in the near future due to increasing the two villages. Apart from that, analysis of As in rice
anthropogenic inputs in the river basin may cause grains of five popular varieties, collected from residents
irreparable ecological approach. of two villages, presented occurrence of high As
Key words: Hydrological status of river, Alluvials/ sediment concentrations in rice grains viz- Gosai (0.95 mg kg-1),
transport, Potential heavy metal, Ecological approach. Satabdi (0.79 mg kg-1), Banskathi (0.60 mg kg-1), Kunti
(0.47 mg kg-1) and Ranjit (0.29 mg kg-1). Furthermore,
SVI/O-19 Gosai and Satabdi were found the most popular
varieties being consumed by local people. This survey
Arsenic Hazard Assessment in based assessment is a primary observation needed to
Groundwater-Soil-Rice System in Two be further immediate action for arsenic remediation.

Villages of Bengal Delta Basin, West Key words: Average daily dose, Arsenic, Groundwater,
Hazard quotient, Rice, Soil.
Bengal, India
Munish Kumar Upadhyay1* , Arnab Majumdar 2 ,
Anil Barla2, Sutapa Bose2 and Sudhakar Srivastava1 Relative Effectiveness of Different
Institute of Environment & Sustainable Development, Iron Complexes in Functional Iron
Banaras Hindu University, Varanasi-221005, Uttar Pradesh,
INDIA; 2Earth and Environmental Science Research
Availability in the Rapseed Plants
Laboratory, Department of Earth Sciences, Indian Institute
of Science Education and Research, Mohanpur-741246, West Rajesh K. Tewari
Bengal, INDIA, Emails: munish007up@gmail.com, Department of Botany, University of Lucknow, Lucknow-
arnabmajumdar891@gmail.com, barla.neal@gmail.com, 226007, INDIA, Email: rktewari_bot@yahoo.com
sutaparai@gmail.com, sudhakar.srivastava@gmail.com
Iron (10 µM) in complex with aromatic ligands
Arsenic (As) problem in groundwater is a global (10 µM) such as salicylic acid, 8-hydroxyquinoline, (+)-
menace since last three decade. Arsenic can also be catechin did not provide enough Fe to plants, which
build up in soil and crop plant through irrigation process. was indicated by decreases in the concentrations of
West Bengal is an important As pollution hotspot in chlorophyll and carotenoids, activities of catalase and
India. To understand the actual reality about As problem, ascorbate peroxidase in the leaves and activated ferric
present study conducted and demonstrated As chelate reductase activity in the roots. These plants
concentrations in soil, groundwater and rice grain exhibited cell death and accumulated ROS. Moreover,
samples of two remote villages, Sarapur and Chinili, they also enhanced DNase, protease and caspase-3-
Nadia district, West Bengal, India. The study was like activity. Iron (10 µM) in complex with juglone (10
conducted in Sarapur and Chinili villages of Dewali µM) was highly toxic as Brassica napus plants
Gram panchayat in Nadia district, West Bengal, India. collapsed within an hour of treatment. However, at 10
The study also comprised a survey among people µM Fe and 2 µM juglone supply plants did not show
residing in these two villages to correlate the knowledge any toxic effect. While B. napus plants treated with
among villagers about the problem with the observed aromatic organic ligands accumulated enough Fe to
results. The data of consumption of rice per day in the support normal metabolic function, they exhibited Fe
survey was used for the measurement of average daily deficiency-like effects. Iron (10 µM) in complex with
dose (ADD) and hazard quotient (HQ). The results non-aromatic organic ligands (10 µM) such as EDTA,
revealed the presence of As in high concentrations in citrate and deferoxamine supplied sufficient functional
groundwater [124.50 µg L-1 (Sarapur) and 138.20 µg Fe to the plants for their metabolic function and growth.
L-1 (Chinili)] and soil [47.7 to 49.3 mg Kg-1 (Sarapur) These plants did not produce any visible toxic effect.
and 57.5 to 62.5 mg Kg-1 (Chinili)]. So, people with
Key words: Iron complexes, EDTA, Citrate, 8-
skin related As toxicity symptoms were also stated in
hydroxyquinoline, (+)-catechin, Juglone, salicylic acid.

142 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVI/O-21 SVI/O-22

Industrial Water Pollution in A Sustainable Phycoremediation

Bangladesh and its Mitigation using the Coupled Lipid Production Efficiency
Catalyst under Ambient Condition in Green Algae under Point Source
Md. Kamal Hossain*, Umme Sarmeen Akhtar and
Mohammad Moniruzzaman Atul Kumar Upadhyay, Ranjan Singh and D.P.
Bangladesh Council of Scientific and Industrial Research Singh*
(BCSIR), Dr. Qudrat-i-Khuda Road, Dhanmondi, Dhaka - 1205,
Department of Environmental Science, Babasaheb Bhimrao
Bangladesh, Email: kamalbcsir@gmail.com
Ambedkar University, Lucknow-226025, INDIA, Email:
Bangladesh is one of the most populous countries dpsinghbbau@gmail.com, upadhyay.eb@rediffmail.com
in the world, facing challenges in its every aspect. Water crisis and limited fossil fuel reserves are
Industrialization has accelerated in more recent years. two major global concerns for the sustainable world.
Industrial sector now contributes more than 28% of Fast globalization and population increase put a gigantic
the Gross Domestic Product (GDP), from around 11 load on the environment to fulfill the demand of fresh
per cent in the mid-1970s. Bangladesh Economic water and energy. The water reserves are continuously
Review, manufacturing remained the largest sub-sector polluted by different types of waste which is not safe
contributing to 17.78% of the GDP in 2009-10. Among for drinking (as well as for human health). A number
them more than 58% are concentrated in and around of attempt and policies were set forth to tackle these
Dhaka city. Textile industry uses large quantity of water problems yet failed due to high cost, unsustainable
in its production processes and highly polluted and toxic nature and ignorance. Thus, there is pressing need for
waste waters are discharged into sewers and drains development of such technology which synergistically
without any kind of treatment. The textile dyeing treats waste water and satisfied the demand of fuel.
industries of generate large amount of effluents, sewage In this view, algal based technology could be a
sludge and solid waste materials everyday which are protective measure. To achieve the goal, four stage
being directly discharged into the surrounding channel, constructed wetland system has been designed. The
agricultural fields, irrigation channels, surface water newly designed system consists of four rectangular tank
and these finally enter in to water bodies and ultimately (2.4 x 1.2 x 0.6 m). The tanks are made up of concrete
goes to food change and living lifer various severe fitted with poly vinyl chloride (PVC) sheet at the base
including carcinogenic disease. Extensive research is to avoid seepage. The tanks were interconnected with
urgently need to treat this waste water for recycling pvc pipe of definite length and equipped with wood
and re storing and reusing this water for agricultural spinner for manual stirring of the algae. In the wetland
and fisheries sector as well as many other sector. algae were grown to treat the waste. The waste water
Among the various treatment technique catalytic collected from four different highly contaminated area
decomposition of industrial waste water is very of Lucknow, Unnao, Kanpur and Varanasi. Results
important. Recently various polymorph of TiO2 is play revealed that the waste water collected from
vital role for degradation of waste water as well as Vijaynagar, Jajmau of Kanpur, CETEP of Unnao and
reduction CO2 which is ultimately impact on climate Kukrail Nala of Lucknow are highly polluted with zero
change as well as global warming. In this experiment dissolved oxygen and high concentration of solids,
we chose methylene blue and 4CP as a model dye. As nitrate, phosphate, chloride and heavy metals as
synthesis TiO2 more than 70% highly efficient compare compared to EPA standard. The waste water collected
to commercial catalyst. XRD, TEM, SEM, GC, Solar from different sites will be treated with algae and
simulator are used for detailed structural checked the algal compatibility in the production of lipid
characterization and application. and further their ecotoxicological analysis will also be
Key words: Bangladesh, Textile industries, Climate change, analyzed under mono algal species and consortia of
Catalyst, Dye degradation, Waste water. algae separately.

Abstracts 27-30 November, 2018, NBRI, Lucknow 143

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Key words: Algae, Waste water, Lipid, Constructed wetland, India, Andhra Pradesh and Karnataka are suffering
Phycoremediation. with ground water As contamination. Low level of As
(up to 17 µg l-1) has also been reported in Tamil Nadu
SVI/P-1 from South India. Many of the states like Jammu and
Kashmir, Uttarakhand, Odisha, Gujrat, Kerala,
Increasing Ground Water Arsenic Telengana, Goa etc. are still unexplored for ground
Contamination in India: A Future water As contamination. Thus, according to current
Danger reports out of 640 districts in India, 141 districts are As
affected (As >10 µg l-1), and 120 are above 50 µg l-1.
Seema Mishra1,2*, Sanjay Dwivedi2, Amit Kumar3, Owing to the toxicity of As to humans as well as its
Reshu Chauhan 2 , Surabhi Awasthi 2 , and R.D. impact on crop productivity As contamination in ground
Tripathi2 water present a great national challenge.
Universität Konstanz, Mathematisch-Naturwissens Key words: Arsenic, Ground water arsenic contamination,
chaftliche Sektion, Fachbereich Biologie, Postfach M665, Indian states.
D-78457 Konstanz, GERMANY; 2Plant Ecology and
Environmental Science Division, CSIR-National Botanical SVI/P-2
Research Institute, Lucknow-226001, INDIA; 3Department
of Botany, Lucknow University, Lucknow-226007, INDIA; Appraisal of Heavy Metals in
UFZ-Helmholtz Centre for Environmental Research, Roadside Soil from Surroundings of
Department of Analytical Chemistry, Permoserstr 15, D-04318
Leipzig, GERMANY, Email: seema_mishra 2003@yahoo.co.in Ropar Wetland, Punjab, India
India is consisting of 29 states and 7 union Sakshi1*, Avinash Kaur Nagpal 1 and Inderpreet
territories, including a national capital, Delhi. Elevated Kaur2
concentration (>10 µg l-1) of arsenic (As) in ground
water of many states of India has become a major Department of Botanical and Environmental Sciences, Guru
concern in recent years. Up to now about 0.2 million Nanak Dev University, Amritsar-143005, Punjab, INDIA;
Department of Chemistry, Guru Nanak Dev University,
ground water samples have been analyzed for As
Amritsar-143005, Punjab, INDIA, Email: svsharma69@
contamination from all over India by various researchers yahoo.com, avnagpal@rediffmail.com, inderpreet11@
and Government agencies. About 90% of these cover yahoo.co.in
only the Eastern part of India while several states and
union territories are still unexplored. However, from In the present study, an attempt was made to
the available data, ground water of eighteen Indian determine the concentration of various heavy metals
viz. cobalt, cadmium, lead, zinc, copper and chromium
states and three union territories has been found to be
in roadside soil samples from the areas surrounding
As contaminated to different extents through natural
Ropar wetland, Punjab, India. Soil samples were
or anthropogenic origin. Among these, As >300 µg l-1
collected from three locations along roadsides of the
has been reported from at least one locality representing
areas surrounding Ropar wetland in March,
fourteen states. The maximum level of As (7350 µg l- 2013.Contents of different heavy metals were assessed
) in ground water has been reported from a highly using flame atomic absorption spectrometer. It was
industrialized area, Patancheru in Medak district of observed that contents of different heavy metals in
Andhra Pradesh. However, the gravity of problem is roadside soil samples ranged as 1.14 to 14.20 mg kg-1
more in West Bengal followed by Bihar and Uttar for Co, 0.26 to 1.10 mg kg-1 for Cd, 5.10 to 14.13 mg
Pradesh. Five out of eight North-Eastern states are kg-1 for Pb, 20.76 to 45.07 mg kg-1 for Zn, 9.16 to 15.16
also affected by As contamination. Manipur is ranked mg kg-1 for Cu and 1.30 to 16.35 mg kg-1 for Cr. Order
first and Assam as second followed by Arunachal of different metals based on their metal contents was:
Pradesh, Tripura and Nagaland. The ground water in Zn (31.36 mg kg-1) > Cu (11.82 mg kg-1) > Co (9.54
these regions is naturally As enriched, and therefore mg kg-1) > Pb (8.38 mg kg-1) > Cr (7.04 mg kg-1) > Cd
wide spatial distribution of As has been found in these (0.68 mg kg-1). Further, it was found that 33% samples
areas. In North India, Punjab and Haryana and in South

144 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

showed Pb content above typical soil concentration of diatom studies not only help in a realistic assessment
10 mg kg -1 , whereas 67% samples had higher of pollution but it also assists in carbon sink potential.
concentrations of Co than the typical concentration of Key words: Algae, Diatom, CO2, Pollution, Futala lake.
8 mg kg-1. All samples showed Cd content to be much
higher than the typical soil concentration of 0.06 mg SVI/P-4
kg-1. The high concentrations of these metals can be
attributed to vehicular emissions. Effect of Arbuscular Mycorrhizal
Key words: Heavy metals, Flame atomic absorption Fungi on Growth and Cadmium (Cd)
spectrometer, Roadside soil samples.
Uptake by Solanum nigrum Grown in
SVI/P-3 Cd Contaminated Soil
Diatoms as an Important Indicators of P. Sharma1, H.P. Singh1* and D.R. Batish2
Pollution, Futala Lakestudy, Nagpur, 1
Department of Environment Studies, Panjab University,
India Chandigarh, INDIA; 2Department of Botany, Panjab
University, Chandigarh, INDIA, Email: hpsingh_01
M.P. Meshram*, K.J. Cherian and S.D. Dhyani @yahoo.com
CSIR-National Environmental Engineering Research It is well known that plant–microbe interactions
Institute, Nagpur-440020, INDIA, Email: m.manisha1029 in the rhizosphere play an important role in soil fertility
@gmail.com and plant health. Arbuscular mycorrhizal fungi (AMF)
The rising carbon dioxide (CO2) emission leading are the most important microbial symbioses for most
to global climate change is one of the greatest plants roots that facilitate nutrient uptake, water
environmental challenges that the world faces today. relations and above-ground productivity in exchange
Most phytoplankton mainly diatom algae is one of the for organic carbon from their host plants. They can
marine and freshwater plants having higher enhance plant growth and resistance to toxicity
photosynthetic efficiencies than any terrestrial plants produced by heavy metals, affect their bioavailability
and is able to capture carbon more efficiently. Algae in soil and uptake by plants and thus have emerged as
utilize bicarbonate directly or indirectly, by its the most prominent symbiotic fungus for contribution
disassociation into carbon dioxide and in so doing cause to phytoremediation. A pot experiment was conducted
an increase in pH of the water and precipitation of in a dome to determine the effect of AMF association
calcium carbonate. Diatoms have the ability of to do with the roots of Solanum nigrum in soils with different
this, that within a dense surface bloom an intense
concentrations of cadmium (0, 10, 25, 45 mg Cd kg-1
carbondioxide demand occurs. In western area of
soil). Control and two experimental sets, one having
Nagpur, ancient Futala Lake exists since 200 years,
different concentrations of cadmium and the other
spread over 60 acres and was selected as the study
having different concentrations of cadmium and AMF
area. In this context an attempt was made to study the
diatom diversity of Futala lake. The surface water (from plant roots) were set up. Growth, biomass,
samples were collected and monitoring was carried photosynthetic efficiency, Cd uptake and accumulation
out seasonally during Jan. 2014 to Dec. 2014. Collected by the plants were measured after six weeks. A better
materials were mounted with Iodine solution and accumulation and tolerance of Cd in the AMF-treated
observations were recorded under the microscope and plants was seen as compared to the control plants and
identified the taxa as per standard literature and the experimental plants with Cd only. The results
encouraging results have been obtained. The 21 genus indicated that S. nigrum associated with AMF
of Diatoms were recorded. Thus the Diatom population effectively enhanced the Cd uptake by plants and offer
is represented with 41 species belonging to 21 genus a novel strategy in microbe-assisted phytoremediation
of which 5 forms were of pollution indicators as per for Cd-contaminated soils.
palmer index. However, the species belonging to Key words: AMF, Hyperaccumulator, Heavy metals,
Bacillariophyceae were more, it is clear that the Rhizosphere, Toxicity.
diatom density was higher in the Futala Lake. Therefore

Abstracts 27-30 November, 2018, NBRI, Lucknow 145

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)


Assessment of Organochlorine Assessing Accumulation of Heavy

Pesticide Residues in the Leaves of Metals in the Bushehr Province Shore
Medicinal Plants of Bathinda City, via Magnetic Properties of Sediment,
Punjab Leaf and Root of Avecina marina
Prashant Singh and Puneeta Pandey* Shadi Karbalaei Hassan 1* , Fateme Kardel 2 ,
Department of Environmental Science and Technology, Hamide Rashid3 and Jelle Hofman4
School for Environment and Earth Sciences, Central 1
Department of Marine and Ocean Sciences, University of
University of Punjab, City Campus, Mansa Road, Bathinda, Mazandaran, Babolsar, Mazandaran, IRAN; 2Department of
Punjab-151001, INDIA, Email: prashantsingh1114@gmail. Environmental Sciences, Faculty of Sciences, University of
com, puneetapandey@gmail.com Mazandaran, P.O. Box: 416, Babolsar, Mazandaran, IRAN;
Geological Survey of Iran, Azadi Square, Meraj Avenue,
India is an agriculture-dependent country where
13185-1494, Tehran, IRAN; 4Lab of Environmental and Urban
the use of pesticides is very common to control pest Ecology, Department of Bioscience Engineering, Faculty of
outbreak and avoid crop damage due to pests. Sciences, University of Antwerp, Groenenborgerlaan 171,
Organochlorine pesticides (OCPs) are Persistent 2020 Antwerp, BELGIUM, Email: ShadiKarbalaei@gmail.com;
Organic Pollutants which are toxic in nature and have f.kardel@umz.ac.ir
the tendency to bioaccumulate in lipids or fatty tissues.
In the present research, a magnetic measurement
The present study reports the estimation of OCP
method which is a novel, inexpensive, reliable and fast
residues in leaf samples of two endemic medicinal
method was used for assessing bioaccumulation of
plants i.e. Azadirachta indica (Neem) and Dalbergia
heavy metal pollution in root, bark, and leaf of Avicenna
sissoo (Sheesham) in Bathinda city, Punjab. The study
marina and sediment in Bushehr province shores, Iran.
has shown the contamination of these leaf samples with
For assessing atmospheric deposition, the magnetic
a total of 9 pesticides i.e. á-BHC, â-BHC, ã-BHC, ä-
properties and heavy metals were measured for
BHC, Heptachlor, Aldrin, Heptachlor epoxide,
washed and unwashed leaf of Avicenna marina. The
Endosulfan I and Endrin. The concentration of
statistical results revealed that magnetic susceptibility
pesticides residues ranged between 1580.01-22061.51
of unwashed leaves for polluted area was significantly
ng g-1 for neem samples and 1547.41-8965.13 ng g-1
higher than reference area (p< 0.01) which indicating
for sheesham samples. The results showed maximum
a higher atmospheric deposition in the polluted area.
contamination of both the samples with Endrin followed
Magnetic susceptibility of sediment was > root>
by Aldrin. The concentrations of ã-BHC and ä-BHC
unwashed leaves> washed leaves and tree bark. This
residues was found to be minimum in both the samples.
result indicates that the magnetic particles did not
The study highlights the importance of continuous long-
translocate from sediment and root to bark and leaf of
term monitoring of the affected environment in the
mangrove trees. The chemical composition of leaves
showed that the concentration of B, Ba, Ca, Cr, Fe,
Key words: Persistent Organic Pollutants (POPs), Mg, Mn, Na, Ni, Pb, Rb, Se, Sr, Th and V in unwashed
Organochlorine Pesticides (OCPs), Bathinda city, Leaves. leaves were higher than washed leaves, and among
these metals the concentration of Ca, Fe, Ni, Na, and
Se were significantly higher in unwashed leaves
compared to washed leaves (p<0.05). A positive and
significant correlation was found between magnetic
susceptibility of leaf and Fe, Ba, Mg, Ni, Rb, Se, Sr,
Mn, Ca, and Na which indicate the source of metal in
the area. According to results obtained in this research,
it can be concluded that magnetic properties of

146 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

sediment can be applied for monitoring of heavy metal decreased biosotption of Pb and Cd, while other anions
pollution in sediment, and the magnetic properties of and cations did not show significant effect. Maximum
leaf and bark for monitoring of atmospheric deposition. desorption of Pb and Cd was achieved in the presence
Moreover, the magnetic properties of sediment and of EDTA and HNO3, respectively. Results also showed
mangrove organs (root, bark, leaf) can be applied for that the test biosorbent could be repeatedly used up to
the evaluation of ecological impacts of heavy metal on six biosorption/desorption cycles without significant loss
mangrove trees, and for conservation of this sensitive of its initial metal adsorption capacity. An analysis of
ecosystem in the shore regions. biosorption of Pb and Cd by using free living,
Key words: Heavy metal, Boushehr shore, Magnetic immobilized living and non-viable forms of N.
properties, Bioaccumulation, Mangrove trees. muscorum revealed that immobilized biomass of N.
muscorum was able to remove more metal as compare
SVI/P-7 to other forms.
Key words: Adsorption isotherms, FTIR spectra, Heavy
Biosorption of Lead and Cadmium by metals, Kinetics, Nostoc muscorum.
Cyanobacteria and Optimization of its
Biosorption Potential
Role of Algae in the Assessment of
Sonal Dixit1* and D.P. Singh2
Pollution Status of River Ganga at
Department of Botany, University of Lucknow, Lucknow-
226007, INDIA; 2Department of Environmental Science, Kanpur, India
Babasaheb Bhimrao Ambedkar University, Raibareilly Road,
Lucknow-226025, INDIA, Email: sonal.env@gmail.com, Vinod Rishi 1 , Bindeshwari Prasad 2 * and A.K.
dpsingh_lko@yahoo.com Awasthi3
The present study relates to the use of Faculty of Science and Environment, Mahatma Gandhi
cyanobacterium Nostoc muscorum as a model system Chitrakoot Gramodaya Vishwavidyalay, Satna, M.P., INDIA;
for removal of lead (Pb) and cadmium (Cd) from aquatic Department of Zoology, Sukdev Singh Luvkush PG College,
systems and influence of different factors on the Baberu, Banda, U.P., INDIA; 3Department of Botany,
Brahmanand College, Kanpur, U.P., INDIA, Email:
biosorption process. The maximum sorption of Cd
vinod.rishi25@gmail.com, bindeshwari1456@gmail.com,
(83.2%) and Pb (90.3%) was achieved at 60 and 80 ashok.awasthi15@gamil.com
ìgml-1 initial concentrations of respective metal, within
30 and 15 min, pH 5 and 6 respectively, with optimum Algae are the morphologically diverse group of
cyanobacterial biomass (2.8 g L -1 ) and at 40 o C autotrophic organisms. They are found in almost all
temperature. A significant proportion of Cd and Pb kinds of habitats and are especially flourished in the
removal was mediated by surface binding of metals organically polluted waters. Some algae highly tolerated
(79.2% Cd and 85% Pb), rather than by intracellular to the organic pollution and also act as indicator of such
accumulation (4% Cd and 5.3% Pb). N. muscorum kind of pollution. Algal samples were collected from
has maximum amounts of metal removal (qmax) capacity two sampling sites viz. Bithoor (Upstream) and Jajmau
of 833 and 666.7 mg g-1 protein for Pb and Cd, (Downstream) of river Ganga at Kanpur during the
respectively. The kinetic parameters of metal binding period from March 2016 to February 2017 and total 51
revealed that adsorption of Pb and Cd by N. muscorum genera spread over 228 species belonging to the class
followed pseudo-second-order kinetics, and the Chlorophyceae, Bacillariophyceae, Cyanophyceae and
adsorption behaviour was better explained by Langmuir Euglenophyceae have been identified. The most
isotherm model. The surface binding of both the metals dominating group was Cyanophyceae followed by
was apparently facilitated by the carboxylic, hydroxyl, Bacillariophyceae, Chlorophyceae and Eugleno-
and amino groups as evident from Fourier transform phyceae. During the present investigations 34 genera
infrared spectra. The presence of anions and cations and 36 species were identified as indicator of organic
showed that existence of Ca and EDTA resulted in pollution on the basis of Palmer’s Pollution Index (PPI)

Abstracts 27-30 November, 2018, NBRI, Lucknow 147

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

for the assessment of pollution status of river. The PPI hyperaccumulators and enhanced Fe, P, K, and Zn
of both genus and species for Bithoor were 29, 28 and uptake. Different plant growth responses to As among
Jajmau 31, 32, respectively. Thus, the river Ganga at hyperaccumulators PV and PM and non-
Kanpur was highly polluted due to the organic pollution. hyperaccumulator PE may help to better understand
Key words: Algae, Organic pollution, PPI. why hyperaccumulators grow better under As-stress.
Key words: Hyperaccumulators, Pteris vittata, Arsenic,
SVI/P-9 Phytoremdeidation.

Arsenic-Induced Nutrient Uptake in SVI/P-10

As-Hyperaccumulator Pteris vittata Accumulation and Distribution of
Xue Liu1, Changhong Liu1*, Yungen Liu1 and Lena Arsenic and Phosphorus in Typha
Q. Ma1, 2 under Arsenic Stress
Research Center for Soil Contamination and Remediation,
Southwest Forestry University, Kunming, 650224, CHINA; Wei Ren2, Yuan Gao1*, Wang Yan2, Yungen Liu 2
Soil and Water Science Department, University of Florida, and Lena Q. Ma1
Gainesville, FL, 32611, UNITED STATES OF AMERICA, 1
Email: bairuixuege@126.com Research Center of Soil Contamination and Remediation,
Southwest Forestry University, Kunming, 650224, CHINA;
Pteris vittata (PV; Chinese Brake fern) is the 2
College of Ecology and Soil & Water Conservation,
first-known As-hyperaccumulator, The success of Southwest Forestry University, Kunming, 650224,
phytoremediation depends on many factors including CHINAEmail: greta_17@163.com
plant biomass, and soil As concentration and availability Arsenic (As) is of environmental concern due to
to plants. However, high biomass production of its toxicity and ubiquity in the environment. In soils, As
hyperaccumulators is a key factor. Therefore, it is is often present in its oxidized form arsenate (AsV),
important to explore ways to increase plant biomass to which is a phosphate (P) analog, sharing similar
improve its phytoremediation efficiency. It is known chemical proper-ties and behavior. The Typha
that arsenic (As) promotes growth of As- orientalist Presl is the research object. This experiment
hyperaccumulator Pteris vittata (PV), however, the was conducted using the pot culture and indoor
associated mechanisms are unclear. Here we examined simulation systems with different As concentrations.
As-induced nutrient uptake in P. vittata and their Choosing seedlings with the most same conditions and
potential role to enhance plant growth in sterile agar keeping roots being flooded to simulate the growing
by excluding microbial effects. As hyperaccumulator environment of wetland. The As concentrations in
P. multifida (PM) and non-hyperaccumulator P. sediment were 0, 50, 100, 150, 200, 400, 600 mg kg-1.
ensiformis (PE) belonging to the Pteris genus were After 45 d of cultivation, the plants height, dry-weight
used as comparisons. The results showed that, after of above-ground and underground and As content were
40 d of growth, As induced biomass increase in determined. The results show that: 1) different As
hyperaccumulators PV and PM by 5.2-9.4 fold concentrations affected the uptake of P and As in
whereas it caused 63% decline in PE. The data Typha. With the increase of As concentration in the
suggested that As played a beneficial role in promoting sediment, plant P and As uptake were first increased
hyperaccumulator growth. In addition, and then decreased, which may relate to the biomass
hyperaccumulators PV and PM accumulated 7.5 fold of plants; 2) the P accumulation by Typha in the shoots
more As, Fe, and P than the non-hyperaccumulator was significantly higher than that of the roots; and 3)
PE. In addition, nutrient contents such as K and Zn the P transport coefficient in Typha from roots to shoots
were also increased while Ca, Mg, and Mn decreased was higher than that of As.
or unaffected under As treatment. This study
Key words: Wetland pollution, Arsenic, Phosphorus,
demonstrated that As promoted growth in
Distribution, Accumulation.

148 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVI/P-11 SVI/P-12

Potential of Decentralized Collegial Microbial Model for Arsenic

Approaches in Sewage Treatment Removal and Plant Growth
Promotion: A Novel Approach
Aditi Roy and Pankaj Kumar Srivastava
Department of Environmental Sciences, CSIR-National Manyata Sharma1 *, Pankaj Kumar Srivastava 1
Botanical Research Institute, Lucknow-226001, INDIA, and D.C. Sharma2
Email: adi.puja.roy@gmail.com 1
Division of Environmental Technologies, CSIR-National
The conventional sewage treatment systems have Botanical Research Institution, Lucknow-226001, INDIA;
always been considered as a successful approach in Department of Microbiology, Dr. Shakuntala Misra National
treating sewage. However, the centralized approaches Rehabilitation University, Lucknow, INDIA, Email:
are having constraints and complications like inefficient sharmamanyata01@gmail.com
plant design, improper operation and maintenance, lack The demand of safe eco-friendly agricultural
of continuous electricity supply and skilled manpower. practice is increasing all over the world. The
The present scenario of sewage treatment plants microorganisms being used as biofertilizers/
require a total paradigm shift from a centralized to biopesticides not only showed the path to safer
decentralized approaches where the sewage could be agricultural practice but also helps in improving soil
treated at source. Most of the centralized sewage fertility and crop productivity. Heavy metal toxicity
treatment plants use the Activated Sludge Process including arsenic is a major concern now a days due to
(ASP) which is energy intensive and focusing on the its serious impacts on human health. Globally, over 100
disposal rather than reuse. Decentralized process like million people are at risk due to consumption of arsenic
Soil Bio-technology (SBT) provides an eco-friendly and contaminated ground water and food produces. Arsenic
sustainable approach by processing both the solid in drinking groundwater water has emerged as a global
organic wastes and waste-water through microbes and health concern. The reports of arsenic contents in rice
mineral additives. Integration of processes like grains are of immediate concern due to its worldwide
sedimentation, infiltration and biodegradation with consumption. Soil harbors a wide array of microbes,
respiration, photosynthesis and mineral weathering, colonizing in the rhizospheric region and promote plant
SBT helps in the bioconversion of sewage and 90% growth. Microbes present in soil may help in the large
removal of COD and BOD. Unlike other conventional scale bioremediation of contaminated soil. Many of
process, the SBT plant consists of an under-drain layer such plant growth promoting rhizospheric (PGPR)
above which lies a layer of media housing culture where microbes possess tolerance to heavy metals including
the raw sewage is pumped for treatment. Thus, it is arsenic. Such microorganisms are capable of reducing
required to have an alternative approach from a disposal arsenic uptake in plants and also improve the crop yield.
based linear system to a recovery based closed system. In this regard, development of a carrier based
The decentralized approaches for treating sewage allow formulation of bio-inoculant to renovate a promising
flexibility in management and possess greater benefits laboratory documented microbe to a commercially
in terms of technical, economical, environmental and profitable field product can be a technology. Formulation
social aspects. Being maintenance free it provides a characteristically contain active constituent or ingredient
feasible onsite treatment solution that is more reliable, in a suitable carrier supported with additives that may
cost-effective and also comply with the environmental assist in the stabilization and longer shelf-life of viable
standards having better treatment efficiency of cells during storage and transportation of the
municipal wastewater. formulation. Several studies were carried out to evaluate
Key words: Sewage treatment system, Soil bio-technology, the effect of such formulations involving bacteria. But
Centralized treatment, Bioconversion, Activated Sludge the use of fungi or fungi in conjunction with bacteria
Process. can be a novel approach for developing such
formulation. Arsenic tolerant and plant growth

Abstracts 27-30 November, 2018, NBRI, Lucknow 149

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

promoting microbial strains can be isolated for and amidase pathways. Cyanide dihydratases readily
developing a carrier based consortia formulation and convert cyanide to relatively non-toxic formate, does
creating a bio-inoculant arsenic remediation product not require any cofactor and its activity is enhanced by
for their soil applications/field trials. This approach will the presence of heavy metal (Cr+, Fe3+) ions. Native
serve dual purpose in reducing the arsenic uptake by novel microbial communities can be utilised to harness
the plants and promoting plant growth Such carrier these potential enzymes which degrade cyanide
based microbial formulation will nullify/reduce arsenic effectively and can further remediate cyanide from
toxicity and gain safe and sustainable agriculture. contaminated effluents. Additionally, the identified
Key words: Arsenic, Microbes, Consortia, Formulation, Plant micro-organisms can be used as a source of potential
growth promotion. genes for development of transgenic plants having
enhanced cyanide detoxification capability.
SVI/P-13 Bioremediation approach utilizing microbial consortium,
fast metabolizing and transgenic plants will help in
Bioremediation as a Prospective reducing cyanide concentration in effluents below the
Technology to Remove Cyanide from permissible level. As such it will result in development
of a viable and effective integrated bioremediation
Coke Oven Plant Effluent of Steel technology for the cyanide contaminated wastewater
Industry and other matrices.
Key words: Cyanide, Bioremediation, Fungi, Wastewater,
Saloni Rishi1 *, Pankaj Kumar Srivastava 1 , R.D. Steel.
Tripathi1, R.N. Kharwar2 and S.K. Verma2
CSIR-National Botanical Research Institute, Rana Pratap SVI/P-14
Marg, Lucknow-226001, INDIA; 2Centre of Advanced Study
in Botany, Institute of Science, Banaras Hindu University, Remediation of Dyes in Water using
Varanasi-221005, INDIA, Email: rishi.saloni@gmail.com; Biogenic Nanoparticles
drpankajk@gmail.com, tripathird@gmail.com, rnkharwar@
gmail.com, skvermabhu@gmail.com Akanksha Pandey* and Pankaj Kumar Srivastava
Cyanide is a poison and well-known as a Environmental Technologies, CSIR- National Botanical
metabolic inhibitor being released in large amounts (2- Research Institute, Rana Pratap Marg, Lucknow-226001,
20 mg l-1 ) in wastewaters of different industrial INDIA, Email: akanksha.p1995@gmail.com
processes related to metal plating, coke oven, and
With the rapid growth of population and increasing
jewellery industry for gold and silver extraction. Its legal
urbanization and industrialization, the environment
discharge limit as per MINAS is 0.2mgl-1. Since
pollution is becoming a serious concern worldwide. The
cyanide is a fast acting toxin, therefore cyanide-
inevitable and unjudicious release of various types of
containing effluents cannot be discharged without being
pollutants into water bodies from a wide range of
subjected to treatment for its content beyond the
industries and chemical factories has been the main
permissible limit. Chemical treatments are often cost
cause of environment pollution. In order to ensure the
intensive and reckoning additional hazardous chemicals
better quality of people’s living standard, how to ensure
like chlorine to the environment. Bioremediation could
the pollutant-free water resources is one of the most
be a felicitous alternative because of its speed,
difficult challenges in the 21st century. Among various
simplicity, cost effectiveness, and eco-friendly
contaminants, dyes are one of the most widely used
mechanisms. Certain bacteria and fungi metabolize
chemicals that are mainly discharged from industries
cyanides by producing different enzymes like cyanide
viz; textile, cosmetic, paper and leather, etc. Even at
hydratase, monoxygenase, nitrilase etc. Trichoderma
minute content (<1ppm), dyes are posing detrimental
spp. has been demonstrated to produce two important
threat to ecosystem and human health risks. The
cyanide-degrading enzymes, rhodanese and cyanide
wastewater containing dyes is one of the most difficult
hydratase. Fusarium solani under alkaline conditions
industrial wastewaters to treat. Recently,
(pH 9.2-10.7) degraded cyanide by cyanide hydratase

150 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Nanotechnology has emerged as an efficient compounds as root exudates viz., L-arginine, DL-
technology for the remediation of different leucine, DL-methionine may change the chemical and
environmental pollutants from water. Green synthesis biochemical properties of soils and thus, regulating the
of nanoparticles can be done for rapid degrade atoms structure of soil microbial community exists in the
of dyes in wastewater. Various nanoparticles such as vicinity of plant rhizosphere. Root exudates of different
iron, palladium and cerium dioxide using Camellia plant species may promote growth of fungal strains as
sinensis, Boswellta serrata , and Azardirachta indica well. There was a requirement to increase numbers of
extracts have been reported successfully for the viable fungal cell counts in the pure fungal cultures for
remediation of anthroquinone, azo and various synthetic consortium preparation. Above mentioned amino acids
dyes like Rhodamine B, methylene blue etc. Therefore, were used to promote fungal cell counts and resulted
the idea of biogenic green synthesis of nanoparticles in increased fungal growth by one fold log units. Despite
for the remediation of different hazardous dyes from conventional methods, an alternative method based on
wastewater may help in achieving an eco-friendly the transformation and colorimetric quantification of
remediation technology for dyes. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet-
Key words: Nanotechnology, Dye pollution, Remediation, razolium bromide] was used to determine the impact
Green synthesis. of amino acids on the increase of viable fungal cell
counts. It was concluded that amino acids in the form
SVI/P-15 of root exudates may increase viable fungal growth
which was validated by the sensitive evaluation using
Role of Root Exudates to Promote MTT assay in their pure cultures.
Fungal Growth in an Arsenic Remedia- Key words: Arsenic, Fungi, Root exudates, MTT assay,
Amino acids.
ting Consortium and Validation of
Growth using MTT Assay SVI/P-16

Ispreet Kaur 1 *, Pankaj Kumar Srivastava 1 and Monitoring of Arsenic Bioavailable

Kavita Shah2 Fractions in Soils of West Bengal and
Environmental Technologies Division, CSIR-National its Relationship with Soil Properties
Botanical Research Institute, Lucknow-226001, INDIA;
Institute of Environment and Sustainable Development for Reducing Arsenic Contamination
(IESD) Banaras Hindu University Varanasi-221005, INDIA,
Email: ispreetkaur50@yahoo.com Suman B. Singh* and Pankaj Kumar Srivastava
Arsenic is a widely recognized human carcinogen CSIR-National Botanical Research Institute, Rana Pratap
and declared as non-threshold toxic contaminant Marg, Lucknow-226001, INDIA, Email: summi29singh
worldwide. Biomagnification of arsenic in edible parts @gmail.com
of food produces, viz., rice grains is causing increased Arsenic contamination has become one of the
human health risks leading to cancer, etc. CSIR – major environmental concerns due to potential health
National Botanical Research Institute has developed a risk. The major arsenic contamination in soil is resulting
novel fugal consortium with ability to tolerate and from contaminated groundwater and enters to food
remove arsenic from contaminated matrices. These chain system. The arsenic contamination in soil is
arsenic remediating fungal strains have been isolated regulated by its concentration and bioavailability. The
from arsenic-contaminated paddy fields of West Bengal, bioavailable fraction of arsenic is also considered as
India. The aim of the study was to find out the the most significant fraction while assessing the potential
substances that may increase growth of these fungal risk arising from it. The arsenic interactions with various
strains during consortium preparation. Root exudates properties of soil would provide a better understanding
are being produced by plant roots in order to provide of its availability. Therefore the current study was
mechanical support and facilitating water and nutrient carried out for investigation of different available
uptake. The exudation of a wide range of chemical arsenic fractions. The study was also assessed the

Abstracts 27-30 November, 2018, NBRI, Lucknow 151

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

correlation of different soil characteristics with fractions carcinogen. Various strategies have been adopted from
of bioavailable arsenic in contaminated soil. Soil samples time to time to reduce arsenic contamination viz; use
were collected from 10 different sites of West Bengal, of surface water for irrigation, use of physico-chemical
India. Three extractants, such as water, sodium treatment options etc. These days bioremediation
bicarbonate (NaHCO 3 ), di-hydrogen ammonium approaches to reduce the arsenic level at the
phosphate (NH4 H2 PO 4), were used to extract the contaminated sites are gaining popularity being eco-
different bioavailable fractions viz water soluble, friendly and cost effective. Mycoremediation of arsenic
weakly adsorbed, specifically adsorbed and residual contaminated sites has been found to be effective as
of arsenic, respectively. The results showed that the fungi are predominant living microbiota in soil and
relative abundance of As fractions were in the order exhibit various mechanisms to reduce As content of
of water-soluble < weakly adsorbed < specifically the affected sites viz. biosoption, bioaccumulation,
adsorbed < residual. It has been found that the biotransformation, biomethylation/ biovolatilization of
availability of arsenic in the paddy soils is highly and arsenic and tend to reduce its bioavailability to crops.
significantly correlated to pH, EC, available sulphur, Biostimulation is a strategy which can be adopted to
organic matter and available phosphorus of enhance the growth of these native arsenic remediating
contaminated soil. Bioavailable soil arsenic fraction fungi under As stressed soils, which are also nutrient
increased with increasing available sulphur-sulfate, EC deficient. Within this strategy organic matter
and organic matter contents and decreased with amendments are added to enhance the performance
increasing P and Fe content. This study provides new of the soil fungi leading to the better arsenic removal.
insight to arsenic bioavailability in soil and this may lead Rice straw, biochar and cow dung manure are some
with reference to develop an effective soil-specific of such amendments that have been found effective in
remediation strategy to arsenic contaminated regions stimulating the growth of arsenic remediating fungi
of India. leading to better removal of arsenic in contaminated
Key words: Arsenic, Bioavailable fractions, Soil property, matrices (water and soil) compared to unamended
Groundwater, Plant. matrices.
Key words: Arsenic, Bioremediation, Biostimulation, Fungi,
SVI/P-17 Nutrients.

Fungi: Remedey for Arsenic Menace SVI/P-18

Mariya Naseem*, Pankaj Kumar Srivastava and Mycoremediation a Potential Solution

Neha Jaiswal for Arsenic Remediation in Soil
CSIR-National Botanical Research Institute, Rana Pratap
Marg, Lucknow-226001, INDIA, Email: mariyanaseem01@ Priya Dubey*, Sneh Lata and Pankaj Kumar
gmail.com Srivastava
Arsenic is a metalloid belonging to group V of CSIR-National Botanical Research Institute, Rana Pratap
the periodic table. It exists in inorganic (As+3, As+5) as Marg, Lucknow-226001, INDIA, Email: priyadubeyd@gmail.
well as organic forms such as monomethylarsonic acid com, drpankajk@gmail.com
(MMA), dimethylarsinic acid (DMA), trimethylarsine Availability of arsenic in soil is toxic to plants.
oxide and trimethyl arsine (TMA). The inorganic forms Developing countries such as Bangladesh, Cambodia,
of arsenic are more toxic compared to the organic Chile, China, USA, Argentina and India are the worst
forms and As+3 is relatively more toxic than As +5 . arsenic affected countries. Arsenic exposure leads to
Arsenic is a threat to humans worldwide mostly in forty serious threat to human health such as skin lesions,
two countries, especially South Asian region. The skin cancers, neurological, cardiovascular diseases.
sources of arsenic include both geogenic as well as Arsenic contamination in soil occurred due to use of
anthropogenic. Arsenic being a systemic toxicant arsenic containing pesticides, mining activities, wood
affects various organ systems and is a potent preservatives and irrigation with arsenic contaminated

152 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

groundwater. There are some physicos-chemical them, inorganic forms of arsenic i.e. arsenate (AsV)
methods for arsenic reduction or immobilization in soil and arsenite (AsIII) are highly toxic to living organism.
but these methods have some limitations such as These inorganic forms of arsenic are easily taken up
generation of highly toxic waste, as secondary source by the plants. Arsenic contaminated water used for
of contamination and high cost. Therefore, there is need drinking, irrigation purpose and food preparation poses
of less expensive ecofriendly technologies like great threat to public health. In India, arsenic
bioremediation for arsenic decontamination. Arsenic concentration in ground water has been found ranging
in soil should be below 20 mg kg-1 as per FAO and for from<10 ppb to as high as 3192 ppb by several authors.
paddy grains it’s should be below 1 mg kg-1 of dry Environmental protection agency (EPA) has reduced
weight (WHO). Arsenic contamination in soil the maximum contamination level for arsenic in drinking
environment has now gained concern of scientific water from 50 ppb to 10 ppb. In soil background arsenic
community because of its accumulation in rice (above concentrations has been reported from 1 to 40 ppm
its permissible limit) a major staple crop especially in with a mean value of 5 ppm. Due to emerging problem
Asian countries. In this review, mycoremediation of arsenic accumulation in plants, soil contamination
(remediation by fungal biomass) has been reviewed has gained interest. Various plants species have different
for lowering and immobilizing arsenic concentration in mechanisms responsible for As (III) and As (V) uptake,
soil and ultimately its accumulation by plants. Various toxicity and detoxification in plants at physiological and
mechanisms have been reported based on fungal biochemical levels. Its chemistry is governed by
activity to immobilized arsenic through bioaccumulation, oxidation-reduction process, acid-base reactions,
redox reactions, volatilization, methylation, co- absorption-precipitation process, plant uptake and
precipitation and complexation or chelation of arsenic accumulation. Ultimately, such mechanisms for
from the contaminant sites. Among these mechanism reducing arsenic contamination provide the opportunity
methylation or methylation followed by volatilization to safe agriculture practices and hence safeguarding
have been found comparatively more effective arsenic the food supply. The use of bioremediation is a potential
decontamination process. Since fungi have advantage method to reduce arsenic concentration in contaminated
over other microbes due to having comparatively larger areas of Gangetic plains of Uttar Pradesh.
biomass, hyphal network, metabolic competence for Bioremediation process in this regards in an option that
metals, and longer life-cycle. Therefore, methylation offers the possibility to destroy various contaminants
of arsenic using soil fungi concluded as a potential using plants and microbes. The aim of this article is to
alternative to arsenic remediation from soil. review arsenic contamination in soil and its mechanisms
Key words: Arsenic, Mechanism, Mycoremediation, Review, for removal from the contaminated source by the
Soil. potential application of plant and microbes.
Key words: Acid-base reaction, Arsenic toxicity,
SVI/P-19 Bioremediation, Detoxification, Soil contamination.

Soil Arsenic: Contamination in Uttar SVI/P-20

An Application of Various Biogenic
Richa Mishra*, Neha Jaiswal and Pankaj Kumar Nano Materials for the Remediation
Srivastava of Arsenic Contaminated Water
CSIR-National Botanical Research Institute, Rana Pratap
Marg, Lucknow-226001, INDIA, Email: richa7087@gmail.com Sampurna Nand*, Virendra Jaiswal and Pankaj
Arsenic (As) toxicity is a global environmental, Kumar Srivastava
agricultural and health issues. Itis generally found in Department of Environmental Technologies, CSIR-National
trace quantities in rock, soil, water, air and get increased Botanical Research Institute, Lucknow-226001, INDIA,
both due to natural as well as human activities. It occurs Email: nandsam1005@gmail.com
in both organic and inorganic forms. Among both of Arsenic is a naturally occurring metalloid in the

Abstracts 27-30 November, 2018, NBRI, Lucknow 153

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

earth’s crust and is a potential human carcinogen. are more toxic than its organic forms, which is
Geogenic arsenic contamination in groundwater is predominant in arsenic contaminated groundwater. The
prevalent in several countries of the world viz; presence of arsenic in groundwater has been a major
Bangladesh, China, USA, India etc., which is posing public health concern in many countries, such as
serious health hazards such as skin lesions, Bangladesh, India, Thailand, China, USA and Nepal.
carcinogenic aliment in human beings. Several Human exposure of arsenic occurs via arsenic polluted
technologies have been applied for mitigation of arsenic water, through ingestion of arsenic contaminated food
contaminated water such as filtration, coagulation, produces. It may cause ‘arsenicosis’ and several
membrane separation, ion exchange, etc. But these carcinogenic diseases. Biosorption is a treatment
methods are less effective, requiring high material process involving biological oxidation-filtration-sorption
costs, high energy requirements, and generation of processes. Fungi have been recognized as promising
sludge. To overcome these limitations, nanotechnology low-cost adsorbents for arsenic removal from arsenic
has been emerged as a proficient strategy to treat contaminated water. Arsenic removal using fungal
arsenic contaminated water in a more specific and biomass have been found effective after modification
accurate way using biogenic nano materials. of fungal biomass by various modifying agents. There
Nanomaterials (NMs) have higher surface volume due are various modifying agents such as iron oxide, ferric
to their nano size and possessing other relevant chloride, and surfactants (such as hexadecyl-trimethyl
biologically properties. NMs which are being ammonium bromide and dodecylamine). Most of the
synthesized using biological organisms (e.g. microbes studies using fungal biomass (Penicillium
and plants) are referred as biogenic nanoparticles. The purpurogenum, Aspergillus niger, Aspergillus
biogenic synthesis of NMs mainly involves bioreduction fumiguts and Aspergillus flavus) revealed that
and bioprecipitation by polyphenols, peptides, amino modification with different salts of iron have potential
acids and other bioactive compounds obtained from to remove arsenic from the drinking water as well as
living organisms. In the present study, promises and from waste water. Different surfactants as modifying
challenges for synthesis of biogenic nanomaterials from agents have been used for arsenic removal from waste
bacteria, fungi, yeast, algae and plants have been water. Fungi have a specific metal-binding proteins and
discussed to synthesize various size and shape of peptides (such as metallothioneins). Iron oxides have
metallic nanoparticles. Of these biogenic NMs, our aim been reported to have relatively high affinity for several
to apply the plant-mediated synthesis of biogenic inorganic oxyanions. Easy recovery of iron from the
nanomaterials and their potential applications in saturated arsenic loaded fungal biomass by magnetic
removing arsenic from contaminated water as being a separation process added the advantage of using iron
feasible, efficient and ecofriendly. modified fungal biomass.
Key words: Arsenic, Biogenic, Nanotechnology, Key words: Aqueous solution, Arsenic removal, Biosorption,
Bioremediation, Contamination. Fungal biomass, Iron oxide.

SVI/P-21 SVI/P-22

Arsenic Removal from Contaminated Green Hydrogels: Most Promising

Water by Modified Fungal Biomass Alternative to Remove Arsenic from
Soni Chauhan*, SnehLata and Pankaj Kumar
Srivastava Vartika Gupta*, Pankaj Kumar Srivastava and
CSIR-National Botanical Research Institute (CSIR-NBRI), Neha Jaiswal
Rana Pratap Marg, Lucknow-226001, INDIA, Email:
CSIR-National Botanical Research Institute, Rana Pratap
Marg, Lucknow-226001, INDIA, Email: vartika1292@
Arsenic is a toxic metalloid, exists in naturally gmail.com
inorganic and organic forms. Inorganic forms of arsenic Arsenic (As) contamination is a global problem

154 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

now. Arsenic is a naturally occurring toxic metalloid SVI/P-23

found in organic and inorganic forms, widely spread in
the environment. It occurs due to geogenic processes Self Cleansing Properties of Ganga
and anthropogenic activities including industrial, during Mass Ritualistic Bathing on
agriculture, mining etc. Inorganic arsenic generally
composed of trivalent arsenite (As III) and pentavalent
arsenate (As V). In the contemporary times, one of
Sanjay Dwivedi1 , Puneet Singh Chauhan1, Seema
the most accustomed problems is to provide cost
Mishra1 , Amit Kumar1 , Mohan Kamthan2 , Reshu
effective cleaner technology to remove arsenic from
Chauhan1, Surabhi Awasthi1 , Pradyumna Kumar
contaminated water affecting human health worldwide.
Singh 1 , Sumit Yadav 1 , Aradhana Mishra 1 ,
Numerous chemical methods (oxidation/ precipitation,
GeetGovindSinam, Shekhar Mallick 1 , Sanjeev
coagulation, ion exchange, and membrane-based
Kumar Ojha 1 , Sri Krishna Tewari 1 , RudraDeo
separation) have been applied to remove arsenic from Tripathi1 and Chandra Shekhar Nautiyal1 *
water sources. These strategies are simple, but the
disadvantages with these methods affiliate viz. huge CSIR-National Botanical Research Institute, Rana Pratap
amount of toxic sludge, poor settling and aggregation Marg, Lucknow-226001, INDIA; 2Environmental Toxicology
Division, CSIR-Indian Institute Toxicological Research,
of metal precipitates. Among all treatments, the
Lucknow-226001, INDIA, Email: drs_dwivedi@yahoo.co.in
adsorption technique has proven to be efficient
technique which requires low operating and The aim of present study was to evaluate the
maintenance cost and does not produce harmful changes in physicochemical properties, microbial
byproducts. During the last few decades, potential diversity and role of bacteriophages in controlling
utilization of renewable feedstock based hydrogels for bacterial population of Ganga water during mass
heavy-metal adsorption for water remediation has been ritualistic bathing on the occasion of Maha-Kumbh in
continuously rising. Several biopolymers such as chitin, 2013. The BOD, COD, hardness, TDS and the level
starch, cellulose etc. have been used to develop of various ions significantly increased while DO
hydrogels. It readily removes arsenic ions from decreased in Ganga water during Maha-Kumbh. Ganga
contaminated water and may give arsenic free water. water was more affluent in trace elements than Yamuna
Owing to the existence of functional groups i.e. and their levels further increased during Maha-Kumbh
carboxylic acid, hydroxyl, sulfonic acid etc. metal ions which was correlated with decreased level of trace
can penetrate the porous structure of bio-based elements in the sediment. The bacterial diversity and
hydrogels and form stable complexes with these groups. evenness were increased and correlated with the
It involves several mechanisms like electrostatic number of devotees taking dip at various events.
interactions, complex formation, chelation etc. Thus, Despite enormous increase in bacterial diversity during
utilization of green biogenic hydrogels for heavy-metal mass ritualistic bathing, the core bacterial species found
adsorption particularly arsenic should be done to gratify in pre-Kumbh Ganga water were present in all the
safe and clean drinking water to human population living samples taken during Kumbh and post-Kumbh. In
addition, the alteration in bacterial population during
in arsenic contaminated areas.
mass bathing was well under the 2 log units, which can
Key words: Arsenic contamination, Environment, Inorganic be considered negligible. Study of bacteriophages at
arsenic, Hydrogels, Remediation. different bathing events revealed that Ganga was richer
in presence of bacteriophages in comparison to Yamuna
against seven common bacteria found during the Maha-
Kumbh. These bacteriophages have played role in
controlling bacterial growth and thus preventing
putrefaction of Ganga water. Further the abundance
of trace elements in Ganga water might also be a reason
for suppressing the bacterial growth.
Key words: Bacteriophage, Ganga River, Microbial diversity,
Metal contamination.

Abstracts 27-30 November, 2018, NBRI, Lucknow 155

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVI/P-24 on Ganga water has been studied extensively and will

be discussed during conference.
Ganga Water Pollution Due to Key words: Devotees, Floral wastes, Holi dip, Ganga river.
Religious Activities
1 1 1
Sanjay Dwivedi , Seema Mishra , Lalit Agrawal ,
Geetgovind Sinam 1 , Sita Ram Taigor 2 , R.D. Emerging Arsenic Removal
Tripathi Technologies from Ground Water in
Plant Ecology and Environmental Science Division, CSIR- India
National Botanical Research Institute, Rana Pratap Marg,
Lucknow-226001, INDIA; 2UP State Ganga River Sanjay Dwivedi 1 *, Seema Mishra 1,2 and R.D.
Conservation Agency; Under Urban Development
Department, Govt. of Uttar Pradesh, INDIA, Email:
drs_dwivedi@yahoo.co.in Plant Ecology and Environmental Science Division, CSIR-
National Botanical Research Institute, Lucknow-226001,
River Ganga is considered as the soul purifier
INDIA; 2Deen Dayal Upadhyay Gorakhpur University,
and Mokshdayini, means a dip in the Ganga liberates Gorakhpur-273009, U.P., INDIA, Email: drs_dwivedi@
from the cycle of death and rebirth. The Ganga basin yahoo.co.in
houses many historical towns like Rishikesh, Haridwar,
Garhmukteswar, Kannauj, Allahabad, Mirzapur, Drinking water is one of the main sources of
Varanasi and Gangasagar which are important pilgrim arsenic (As) exposure to humans particularly in Indian
centers where several religious activities takes place subcontinent where ground water As contamination is
throughout the year on the bank of Ganga. During common phenomenon. The conventional techniques for
different festivals billions of people come to these places As removal involves chemical precipitation, oxidation
for holy dips called “Ganga snans”. Devotees offer and coagulation which often show lower efficiency,
different kinds of materials like sweets, milk, flowers, consumption of chemicals, production of toxic sludge
leaves, lighted earthen lamps to Ganga, and discard and thus, having many disadvantage. In recent years,
remains of old holy books and idols thereby various organizations have developed a number of As
contaminating the river water. Several communities in removal devices/ units showing good As removal
India throw dead bodies and the remains of bones, after efficiency from ground water (GW). These techniques
crimination, in Ganga as a part of last ritual. More than are novel, eco-friendly, sorbent material based and have
1000 tons of flower and garlands are thrown in the higher sorption capacity of As. These devices/ units
river as offering during worship of Ganga as well as vary in size, filtering mechanisms, and mechanisms of
those used in the temples nearby. At various places operation, therefore based on the size, the devices are
such as Haridwar, Varanasi etc. splendid evening of two type’s: (1) Arsenic Removal Unit (ARU) and
prayer of Ganga is being held during that the devotees (2) Arsenic Removal Plant (ARP). Arsenic Removal
offers flowers and hundreds of floating lighted earthen Units are those, whose inlet are directly connected to
lamps. These processes are more during mass rituals. a hand pump or tube well. It is normally a small domestic
According to an estimate of Kashi Vishwanath temple assembly which can filter up to 500 l of water per day
administration of Vanarasi, in normal days, over 20 and can meet requirement of water for a smaller
quintal of floral waste was being disposed into the section of people. These arsenic removal plant/units
Ganga daily, while during special occasions like are Ceramic micro-filtration membrane unit; AMRIT;
Mondays of the month of Shrawan (July-August), the IITB arsenic filter; DRDO arsenic removal filter;
quantity increased by four to five times. Further each Low cost laterite based arsenic filter; ARI ground
household dispose of their religious material, including water arsenic treatment plant. Arsenic Removal
those used in daily prayer, into the Ganga. Thus, floral Plants (ARPs) has the capability to treat a large quantity
and other religious temple wastes continue polluting of water and can cover a large section of general public.
Ganga water. The impact of different religious activities These technologies have demonstrated good efficiency

156 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

to remove As below WHO permissible limits for region of Ghaghara basin is less arsenic polluted being
drinking water (10 µg l-1) under field conditions in As maximum (4.7ìgl-1) in Mugu district of Nepal. In these
affected areas. districts cereal crops like rice and animal based food
Key words: Arsenic, Arsenic Ground water problem, are the main As exposure to human due to transfer of
Treatments plants. As to higher tropic level through food chain
contamination. The many local inhabitants of these
SVI/P-26 districts are suffering from different disease induced
by arsenic accumulation in their body. The highest
Contamination of Arsenic in Ghaghra amount of arsenic has been detected in their nails
Basin: A Potential Thread to Local followed by hairs and urine.

Inhabitants Key words: Ghaghara basin, Arsenic, Arsenic Ground water

problem, Arsenicosis.
Sanjay Dwivedi 1 , Seema Mishra 1,2 , Vishnu SVI/P-27
Kumar* 1 , Pragya Sharma* 1 , Amit Kumar 1 and
R.D. Tripathi1 Use of Plants and Animals as Bio
Plant Ecology and Environmental Science Division, CSIR- Indicators in Ancient Indian Science of
National Botanical Research Institute, Lucknow-226001,
INDIA; 2Deen Dayal Upadhyay Gorakhpur University, Climatology and Effect of Climate
Gorakhpur-273009 (U.P.), INDIA, Email: vishnukumar772 Change on it
@gmail.com, sharmapragya766@gmail.com
Chronic arsenic toxicity from ingestion of Navneet Shukla* and Vinod Shukla
contaminated drinking water has been reported from 1
Department of Forestry, Dolphin P.G. Institute of Biomedical
many countries of the world, including India. In India, and Natural Sciences, Chakrata Road, Dehradun-248007,
eighteen states and three union territories are facing Uttar Pradesh, INDIA; 2 Department of Geography,
ground water arsenic problem. Uttar Pradesh, one of Munishwar Dutt Post Graduate College, Pratapgarh-230001,
the severely arsenic affected state, is located at north Uttar Pradesh, INDIA, Email: nvneet4@gmail.com,
of India bordering Nepal. The Ghaghara, sub-basin of vinodshukla177@gmail.com
Ganga basin has a total catchment area of 58,634 Sq.km. Like other sciences, the science of Climatology
where two big rivers flowing from the northwest to based on plant and animal behaviour was well developed
the south-east are Ghaghara and Sarda. Up to now in ancient India. It is unfortunate that, now a days, we
the As contamination has been said to be mostly do not pay proper attention to our ancient Indian
confined in Ganga basin originating from Himalaya. sciences. Somehow ancient Indian science have lost
However, recent studies have shown that several district all their utility in the face of the modern sciences. But
situated in Ghaghara basin also have higher level of As this concept will prove false if we try to realize the real
in ground water. The Terai planes of Ghaghara is merits of the ancient Indian sciences. The main
particularly As contaminated. Ghaghara basin covers difference between the modern sciences and ancient
5 districts of Nepal (one is arsenic affected), fifteen of Indian sciences is that, in modern sciences, the deeper
Uttar Pradesh (eleven are arsenic affected) and seven we go the more we have to depend upon costly
of Bihar (two are arsenic affected) in India. These machines and other instruments. Thus, the earth and
arsenic affected districts of Ghaghara basin having the atmosphere had been their laboratory wherein the
different levels of ground water arsenic contamination. plants and animals, other natural objects had to serve
In Ghaghara basin, the maximum arsenic concentration the purpose of instruments and equipment’s. We can
in ground water is reported from Bhozpur district of found some folklores by Ghagh and Bhadduri
Bihar (1654 ìgl-1) followed by Ballia (1310 ìgl-1), Gazipur describing the relationship of phenological characters
(531 ìgl-1), Gonda (510 ìgl-1), Faizabad (350 ìgl-1), Basti of some plants with rain forecasting. Brihat Samhita
(150 ìgl-1 ) and Bahraich (100 ìgl-1 ). The results of of Shri Varahamihira also contains important botanical
different studies revealed that the ground water of hilly data which is itself is an encyclopaedia of astrology.

Abstracts 27-30 November, 2018, NBRI, Lucknow 157

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

We found some temporal variations in weather and (86.90%), nitrogen (64%.16), potassium (72%), total
monsoonal events, while studying present weather organic carbon (202%), chlorine (35%), phenol
conditions using these bio indicators as weather (38.46%), hardness (51.48%) and floride (64.90%).
forecasting tool and comparing them with pre described The microalgae also demonstrated high reduction in
theories given by ancient Indian Rishis. Research need the percentage of different heavy metals in the range
and priorities emphasized on- i. Identification and between 43 to 99% in continuous system. Waste water
inventorization of flora as bio indicator species to supported algal growth which exhibit number of
promote non-destructive study of climate change. ii. dependent and independent metabolism process of the
Establishment of bio indicator gardens for research and uptake and accumulation of heavy metal. Thereby with
educational purposes. iii. Awareness about the recent advances in scientific knowledge, sophisticated
importance of ancient Indian sciences among young techniques and environment awareness microalgae
researchers. could offer an elegant, environment friendly solution to
Key words: Bio indicators, Ancient Indian Science, Plants tertiary and quandary treatments of sewage water.
and animals, Weather forecast, Climate change. Furthermore, harvested algal biomass can be utilized
for several value added products development such as
SVI/P-28 bioenergy, pharmaceuticals, nutraceuticals, genetically
engineered products etc.
Bioremediation of Sewage using Two Key words: Phycoremediation, Biorefinery, Waste-water
Potential Microalgae treatment, Chlorophyceae.

Atul Kumar Singh*, Chitralekha Nag Dasgupta, SVI/P-29

Kiran Toppo and Sanjeeva Nayaka
Phytoremediation Potentiality of Four
Algology Laboratory, CSIR- National Botanical Research
Institute, Lucknow-226001, Uttar Pradesh, INDIA, Email: Fly Ash Tolerant Plants Grown On Fly
atulfc2114@gmail.com Ash Dykes: A Case Study of Obra
Rapid urbanization and increase of municipal Thermal Power Station, Sonebhadra,
sewage resulted in load on conventional sewage disposal
system. The composition of municipal sewage water
mostly contains natural organic and inorganic minerals
Ashutosh Pandey*, Sanjay Dwivedi, Shekhar
as well as man-made compounds. Microalgae utilize
Mallick, Nandita Singh and Vivek Pandey
these wastes as nutritional sources and improve the
quality of sewage water. In the present study, algae Plant Ecology and Climate Change Division; CSIR-National
belonging to Chlorophyceae family showed good and Botanical Research Institute Lucknow-226001, INDIA, Email:
healthy growth in sewage waste water by absorbing apandeymsb@gmail.com
and utilizing organic and inorganic waste. Two isolates Heavy metal contamination in water and soil is a
Scenedesmus quadricuada and Chlorella vulgaris big challenge of 21th century. Due to increasing demand
were used for treatment of sewage water of Bharwara of electricity in India, millions of tons of fly ash is
Sewage Treatment Plant (STP) located in Gomti Nagar, generated per annum from thermal power plants. Fly
Lucknow. More than 6 times improvement in dissolved ash contains high amount of toxic elements which not
oxygen (DO) concentration has been noticed. About only affect the local vegetation, but their accumulation
12.50 reductions were obtained in total dissolved solids in crop plants results in food chain contamination. The
(TDS). Chemical oxygen demand (COD) and biological present study is focused on to assess the
oxygen demand (BOD) was reduced by 33.77% and phytoremediation potentiality of four plants namely
19.62% respectively. After treatment COD and BOD Phragmitis kraka, Saccharum spontaneum, Canna
were found 50.4 and 17.2 mg L-1 which are within the occidentalis and Pteris vittata. These selected plants
limits of standard specified by CPCB norms, 2015. were transplanted on fly ash dykes in the month of
Treatment induced progressive reduction of phosphorus September 2016 and monitored after one year in terms

158 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

of plant height, biomass and heavy metal accumulation the availability of heavy metals. Further, despite the
in different plant parts. The results indicated that fly heavy metal contamination, we observed a very
ash contains significant amounts of Mn (411 mg kg-1), complex and indifferent pattern of bacterial community
Fe (11083 mg kg-1), Cu (41 mg kg-1), Zn (27 mg kg-1) composition along the heavy metal contamination sites.
and toxic Cr (61 mg kg-1), Ni (40 mg kg-1), As (8 mg Overall, we found that c-Proteobacteria had been the
kg-1 ), Pb (4 mg kg -1 ) metals. Among the plants, most abundant bacterial community followed by
Saccharum spontaneum, accumulated highest amount Actinobacteria, Firmicutes, b-Proteobacteria and a-
of Cr (5 mg kg-1) and Ni (13 mg kg-1) in roots, while Proteobacteria. Commemorating all the results, we can
arsenic was found highest in the shoot of Pteris vittata infer that arsenic and other heavy metal contamination
(38 mg kg-1). After one year, the reduction in metal is deteriorating the soil quality and hence warrants
content of fly-ash was found maximum for Cu (65%) immediate attention of concerned soil scientist and
followed by As (64%), Cr (55%), Zn (46%) and Ni agronomists.
(35%), Fe (31%) and Mn (22%). Therefore, the study Key words: Arsenic, Paddy, Proteobacteria, Enzymes.
concluded that the Saccharum spontaneum, and Pteris
vittata are the most suitable plants to develop SVI/P-31
phytoremediation technique for decontamination of fly
ash dykes. Correlation of different parameters/results A Study on Carbon Sequestration
will be presented during the conference.
Potential of Chlorella ellipsoidea
Key words: Fly-ash, Heavy metals, Food chain
contamination, Phytoremediation.
Isolated from Domestic Sewage Water
with Inorganic Source of Carbon
Himangshu Sharma*, Mahdi Ahmed, Pampi
Arsenic Contamination Confines Sarmah and Jayashree Rout
Fertility Indicators of Paddy Soil Phycology and Lichenology Laboratory, Department of
Ecology and Environmental Science, Assam University,
Vijay Kant Dixit, Shashank Kumar Mishra, Sankalp Silchar, Assam (India) Pin-788011, Email: hshimangshu270
Misra, Arpita Singh, Puneet Singh Chauhan* @gmail.com, mahdiahmed438@gmail.com, mailtopampi@
rediffmail.com, routjaya@rediffmail.com
Division of Plant Microbe Interactions, CSIR-National
Botanical Research Institute, Lucknow-226001, INDIA, The potential of microalgae have been well
Email: puneetnbri@gmail.com recognized for its efficient carbon fixation and
Emerging environmental issues related to heavy contribution in substantial biomass production. The
metal contamination in rice draw great concern about present paper highlights the carbon sequestration
the soil quality of paddy farming lands irrigated with efficiency of a freshwater algal species viz. Chlorella
groundwater. Investigating the functioning of soil ellipsoidea isolated from domestic sewage water of
microorganisms exposed to heavy metal contamination Cachar district, Assam (India). Growth response of
is imperative for agricultural soil manipulations. The the aforementioned species was monitored under
current study accentuates the influence of heavy metals varying concentrations of nitrate (1.0, 1.5, 2.0 g l-1),
on microbial activity and community composition in phosphate (0.03, 0.04, 0.05 g l-1) and CO2 (g) (20, 40,
arable soil of West Bengal State of India. The result 60, 80,100 ml min-1). The value of pH ranges from
revealed that the fertility indicators (activity of all soil 5.92 to 9.93. The maximum chlorophyll a content and
enzymes) and growth-limiting factors (soil N and P) cell density in case of Chlorella ellipsoidea was
were negatively correlated with the heavy metal stress estimated to be 4.79 µg ml-1 and 984 cells/ml×104 during
except the soil total organic content which demonstrated study period respectively. The maximum protein and
significant positive correlation with the heavy metals. carbohydrate content was observed to be 44.99 µg ml-
In case of functional diversity of soil, all the considered and 118.97 µg ml-1, respectively. The highest biomass
diversity indices exhibited no specific pattern along with productivity and CO2 fixation rate was found to be 64.3

Abstracts 27-30 November, 2018, NBRI, Lucknow 159

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

(mg l-1 day-1) and 120.89 (mg l-1 day-1), respectively. Key words: Wastewater, Emerging chemicals,
This microalga seems to be a promising candidate for Phytoremediation technology, Constructed wetland.
effective carbon sequestration ability and in general,
this study may be advanced further for strategic SVI/P-33
optimization of higher microalgal biomass yield, lipid
production and other value added products.
Strategies for Arsenic Reduction in
Key words: Carbon sequestration, Microalga, Chlorella
Crops for Sustainable Environment and
ellipsoidea, Sewage. Agriculture
SVI/P-32 R.D. Tripathi 1*, R. Chauhan1 , S. Awasthi 1, P.K.
Singh, M. Shri1, P. Verma1, S. Dwivedi1, S. Mishra2,
Removal of Emerging Contaminants P.K. Srivastava1 , B. Adhikari 3, P.K. Trivedi 1 and
of Municipal Wastewater through D. Chakrabarty1
Sustainable Phytotechnologies 1
CSIR-National Botanical Research Institute, Rana Pratap
Marg, Lucknow-226001, Uttar Pradesh, INDIA; 2DDU
Gurudatta Singh* and Virendra Kumar Mishra University, Gorakhpur, Uttar Pradesh, INDIA; 3Rice Research
Station, Department of Agriculture, Chinsurah, Hooghly,
Institute of Environment and Sustainable Development, West Bengal, INDIA, E-mail: tripathird@gmail.com
Banaras Hindu University, Varanasi-221005, INDIA, Email:
gurudatta.singh2@bhu.ac.in Arsenic is a widely recognized human carcinogen
and declared as non-threshold toxic pollutant
This study mainly discuss the removal of worldwide. The most common routes of arsenic
emerging chemicals in wastewater through exposure to humans are the usages of contaminated
phytotechnologies. The occurrence of emerging water for drinking and irrigation in agricultural field.
contaminants or newly identified contaminants in This leads to arsenic contamination in crops and
municipal wastewater is of continued concern for the vegetables grown on such soil eventually contaminating
health and safety of public. The design parameters of the food chain. In a regime where cultivation of GM
the reported constructed wetlands including the physical crops are not encouraged, selection of low grain arsenic
configuration, hydraulic mode, vegetation species, and cultivars of crops through breeding process, needs to
targeting pharmaceuticals were summarized. The be extensively explored. Rice being a cereal consumed
removal efficiencies of pharmaceuticals under different by half of world’s population, needs special attention.
conditions in the wetlands were evaluated. In addition, Through breeding and selection, a rice cultivar CN1794-
the importance of the three main components of 2-CSIR-NBRI was developed. This rice cultivar while
constructed wetland (substrate, plants and microbes) accumulating low As maintains higher level of
for pharmaceutical removal was analysed to elucidate micronutrients such as Fe, Cu, Zn, Se and Ni.
the possible removal mechanisms involved. The Application of arsenic tolerant bacterial strains
compounds were extracted from municipal wastewater decreased arsenic uptake in shoots and increased
samples by solid phase extraction and analyzed by ultra- stabilization of arsenic in root zone of rice plants, can
performance liquid and gas chromatography couple with also be a cheap and safer strategy. In order to restrict
tandem mass spectrometry (UPLC, GCMS, MS). As in the rice roots a transgenic approach has been
Most of the target compounds were detected with the followed through expression of phytochelatin synthase
concentration of 4.4 ng L -1 to 6.6 µg L -1 . (PCS), CdPCSI, from Ceratophyllum demersum, an
Phytoremediation technology through constructed aquatic As accumulator plant. Rice transgenic lines
wetland was used to remove target compounds such showed enhanced accumulation of As in root and shoot.
as sulhamethox azole and tetracycline, with the removal However, all the transgenic lines accumulated
efficiency of 17% to 98% for sulhamethox azole, and significantly lower As in grain and husk in comparison
12-80% for tetracycline was achieved. Constructed to non-transgenic plant. The higher level of PCs in
wetlands had better efficiency to remove emerging transgenic plants relative to non-transgenic presumably
contaminants from wastewater. allowed sequestering and detoxification of higher

160 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

amounts of As in roots thereby restricting its Stable isotopes occur naturally in environment and
accumulation in grain. Another approach could be of have emerged as promising environmental tracers.
As volatilization through genetically engineered They are safe and non-radioactive, and do not decay,
Arabidopsis thaliana with arsenic methyltransferase thus are suitable natural environmental tracer in
(WaarsM) gene from one of the fungal strain understanding various environmental processes and
Westerdykella auantiaca (MTCC10845), isolated pollutant dynamics in environment. Stable isotopes have
from arsenic-contaminated sites. The WaarsM been used in understanding biogeochemical cycles.
transgenic A. thaliana plant showed enhanced Stable isotopes have been used in different biological
tolerance to AsV and AsIII, compared to wild-type and ecological studies. It can be used to understand
(WT) plants. WaarsM expressing transgenic plants fundamental processes in the biology and ecology of
evolved significant amount of volatile arsenicals (mg-1 arthropods, which range from nutrition and resource
fresh weight) after 48 h of exposure. Further, WaarsM allocation to dispersal, food-web structure, predation,
gene expressed in rice was able to convert toxic feeding behavior, mating, etc. Compound-specific stable
inorganic arsenicals to methylated arsenic species, isotope analysis (CSIA) has been used to study the
therefore, reduce arsenic accumulation in rice grains. fate of various groundwater pollutants, such as
In response to arsenic treatment in hydroponics, monoaromatic and polyaromatic hydrocarbons, MTBE,
WaarsM expressing transgenic lines showed a marked chlorinated ethenes, perchlorate, nitrate, and chromium
increase in arsenic resistance and reduced its (VI). CSIA has also been used in monitoring diffuse
accumulation compared to NT. Also, WaarsM pesticide pollution in river. Metal stable isotope
expressing transgenic Line 1 evolved c.a. 157 ng and signatures can be used as tracers to get a deeper
c.a. 43 ng volatile arsenicals (mg-1 fresh weight) after understanding of environmental geochemistry and to
72 h of exposure to 25 ìM AsIII and 250 ìM AsV, deduce information about composition and history of
respectively. Transgenic line 1, grown in soil irrigated environmental samples and understanding other
with arsenic-containing water accumulates about 50% environmental processes like redox process,
and 52% lower arsenic than the NT in shoot and root, complexation and organic matter binding, biological
respectively; while arsenic concentration in polished cycling, etc. This paper presents a review on potential
seeds and husk of the transgenic line was reduced by use of stable isotopes in biological, ecological and
52% compared to NT. Thus, the present study
pollution studies.
demonstrates that the expression of WaarsM in rice
induces arsenic methylation and volatilization, provides Key words: Stable isotopes, Compound-specific stable
a potential strategy to reduce arsenic accumulation in isotope analysis (CSIA), Environmental processes, Pollutant
rice grain. Suitable combined biotechnological and dynamics.
microbe plant interaction approaches may lead to safer
levels of arsenic in the food crops grown in the arsenic
contaminated soils.
Lichen Use as a Biomonitoring Tool for
Key words: Arsenic, Methyl transferase, Microbes,
Arabidopsis thaliana.
Assessment of Air Quality

SVI/P-34 Kirti Kumari* and Sanjeeva Nayaka

Lichenology Laboratory, CSIR-National Botanical Research
Application of Stable Isotopes in Institute, Rana Pratap Marg, Lucknow-226001, U.P., INDIA,
Environmental Monitoring and Email: kirtikumari64@gmail.com

Pollution Studies Lichens are promising biomonitoring agent of

atmospheric pollution as compared to any other group
Vivek Kumar* and D. Paul of plants. Lichen is a combination of an alga and a
fungus which live together in symbiotic association.
Department of Environmental Studies, North-Eastern Hill
During association, the algal component (phycobiont
University, Shillong-793022, INDIA, Email: naturevivek001
@gmail.com; pauld97@rediffmail.com or photobiont) and fungal component (mycobiont) lose

Abstracts 27-30 November, 2018, NBRI, Lucknow 161

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

their original identity and form the composite thallus, SVI/P-36

which is both morphologically and physiologically
behaves as one entity. For the growth and abundance Phytoremediation of Heavy Metal
of lichens several parameters are required like adequate Contaminated Soils: A Potentially
amount of moisture, light and altitude, unpolluted air
and undisturbed perennial substratum. Alteration in the Promising Clean-Up Technology
atmosphere can bring marked changes in morphological
and physiological features of lichen. Changes in K.K. Tiwari
chlorophyll in lichens is very sensitive to changes in Sophisticated Instrumentation Center for Applied Research
environmental factors including air pollution. Lichens & Testing (SICART), Sardar Patel Centre for Science &
have been found to be very much sensitive to Technology, Vallabh Vidyanagar-388120, Anand, Gujarat,
environmental parameters like temperature, humidity, INDIA, Email: drkktiwari@hotmail.com
wind and air pollutants because they don’t have any Environmental pollution of the biosphere with toxic
vascular system and thus absorb water and nutrients heavy metals has accelerated dramatically since the
passively from their surrounding environment. The beginning of the industrial revolution. Contamination
changes in species composition is a very powerful tool of soils with toxic heavy metals is a widespread
to get information about changes in climate, air quality environmental problem resulting from global
and biological processes. The lichens respond to the industrialization. Some heavy metal remediation
environmental changes by reflecting changes in their technologies have been developed to treat
diversity, abundance, morphology, physiology, changes contaminated soil, but a plant-based remediation
in DNA, accumulation of pollutants etc. Climate change technology, phytoremediation, is a new emerging
and air pollution are two very important fields to study technology. Phytoremediation is green technologies
as they impact not only human beings but also affect utilizing green plants to clean up the environment from
flora and fauna throughout the world. Lichens are very contaminants and has been offered as a cost-effective
useful indicators for long term monitoring of climate and non-invasive alternative to the conventional
change and air pollution and their vulnerability as they engineering-based remediation technique. The
are not only very much sensitive to climate change advantage of the technique lie in making the living plants
and air pollution but also measurable and thus relevant act as a solar-driven pump, which can extract and
for biomonitoring studies. Their response to any change concentrate certain heavy metals from the
in climate or pollution is much faster than any other environment. The process of hyperaccumulation of
biota. Lichens are successfully used to monitor global heavy metals by higher plants is a complex
warming at various places in the world. Lichens are phenomenon. Phytoremediation process includes
important components of ecosystems. Lichen has been phytovolatilization, phytostabilization, and
used as natural indicator of climate change and air phytoextraction using hyperaccumulator plant species.
quality monitoring worldwide including US, Britain, Plants have a range of potential mechanisms at the
Canada, Netherlands, Switzerland, Italy and Israel etc. cellular level that might be involved in the detoxification
But very few such studies have been done in India. and tolerance to heavy metal stress. Technologies with
Key words: Air quality, Lichen, Air pollution, Climate change, the use of new transgenic plants have also improved
DNA damage, Lichen physiology, Bio-indicator. the capacity of biochemical processes such as metal
uptake, transport, accumulation and detoxification of
metal pollutants. The recent research that certain
chelating agents greatly facilitate metal accumulation
by soil-grown plants can make this remediation
technology a commercial in the near future. Thus the
knowledge on the physiological and biochemical
responses helps to adopt different strategies of
purification and improvement of the environment

162 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

through use of plants, which tolerate and accumulate takes place or not by geophysical signatures.
high levels of heavy metals. Microorganisms alter the chemical and physical
Key words: Phytoremediation; heavy metals, properties of soil during its metabolism process. These
phytoextraction, accumulation, translocation changes lead to altered geophysical signatures. Hence,
geophysical methods being non invasive, cost effective,
SVI/P-37 less time consuming and less expensive, provides an
opportunity for using them as an innovative approach
Bioremediation and Monitoring of 2,4- for getting high density spatial and temporal information.
D in Contaminated Soil of Vidarbha This information can be used in mapping contaminated
soil and monitoring its bioremediation.
using Non-Invasive Geophysical Tools:
Key words: Bioremediation, Geophysical, 2,4-D,
A Novel Approach Bioaugmentation, Biostimulation.

K.S. Bramhanwade*, S. Dhyani1 , P. Balwant1, P. SVI/P-38

Pujari1, F. Chiampo2 and A. Godio3
CSIR-National Environmental Engineering Research
Chelants (EDTA, Citric Acid) Induced
Institute (NEERI), Nagpur, INDIA; 2Dipartimento di Scienza Nickel and Chromium Accumulation in
Applicatae Tecnologia - Politecnico Di Torino-Torino, ITALY;
Dipartimento di Ingegneria dell’Ambiente, del Territorioe
Tomato Grown on Industrially Polluted
delle Infrastrutture-Politecnico Di Torino-Torino, ITALY, Soil
Email:ksbramhanwade@rediffmail.com, shalini3006@
gmail.com, d001940@polito.it, pandurangbalwant@ S.N. Pandey* and Sanjoli Tripathi
gmail.com, pr_pujari@neeri.res.in, fulvia.chiampo@polito.it,
Department of Botany, University of Lucknow, Lucknow-
2,4-D (2,4-dichlorophenoxyacetic acid) herbicide 226007, INDIA, Email: snpandey511@gmail.com
is an organic , selective, post-emergence systemic
Industrial effluent 25% (Low) and 100% (High)
herbicide, prominently used for controlling weeds
irrigated soil (silty loam texture, mild calcareous, pH
across India. On spraying the herbicide on weeds only
7.9) evaluated for potentially toxic heavy metals (Ni
40% goes at target while, the rest goes to the soil and
and Cr). This contaminated (Low and High) soils used
contaminates it. As a result it affects the beneficial soil
to grow tomato plants in clay pots (8 kg size) with
microflora and leads to decline in soil fertility.
application of EDTA (10µg) and citric acid (10 µg)
Bioremediation is striking method due to its eco-friendly
twice at the difference of 30 days. Growth, tissue
and economic character. It involves removal of
accumulation of Ni and Cr and some biochemical
contaminants with the help of microorganisms or their
constituents (protein and total chlorophyll contents)
enzymes from environment. To decontaminate soil
observed at day 90 of the growth. The application of
there is a need to remediate it by biological measures.
both the chelants enhanced tissue accumulation of Ni
Therefore bioaugmentation and biostimulation strategies
and Cr in tomato. As compared, citric acid induced
of bioremediation were applied on soil and monitoring
more Cr accumulation than EDTA, whereas EDTA
process. In present study 2,4-D degrading microbes
induced more Ni accumulation than citric acid. Also,
were isolated from soil by regular microbiological
EDTA induced Cr accumulation in tomato. With respect
isolation method and were further used in
to tissue accumulation levels of Ni and Cr, dry matter
bioaugmentation process. Experiment of bioremediation
yield, protein and total chlorophyll contents was
was carried out in column to monitor it by geophysical
observed to be decreased in tomato.
methods. Study mainly focus on monitoring, as it is
necessary to check whether bioremediation actually Key words: Chelants, EDTA, Citric Acid, Contaminated soil,
Protein, Nickel, Chromium.

Abstracts 27-30 November, 2018, NBRI, Lucknow 163

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVI/P-39 and leached down and mixed with the groundwater

after percolation. The result shows that the
Physico-Chemical Characteristics of groundwater quality is threatening with the
Surface Water nearby Industrial contamination of pollutants.
Areas of Balrampur Key words: Balrampur, Groundwater, Indo-Nepal border.

Zaheen Hasan* and D.D. Tewari SVI/P-41

Water Analysis Laboratory, Department of Botany, M.L.K. Physico-Chemical Characterization of

(P.G.) College, Balrampur, INDIA, Email: zaheenhasan666@
gmail.com Rivers in Different States of India: A
Present paper deals about the water qualities Review
occur near the industrial areas of Balrampur city. The
industrial areas of Balrampur city has large number of Sandip Singh Bhatti1*, Jaswinder Singh2, Vasudha
small scale industries as well as one of the largest sugar Sambyal3 and Avinash Kaur Nagpal1
factory i.e. Balrampur Chini Mill Ltd. with one distillery Department of Botanical and Environmental Sciences, Guru
unit. This investigation focus mainly on the effluents Nanak Dev University, Amritsar-143005, Punjab, INDIA;
released from BCM Ltd. which is running during the Department of Zoology, Khalsa College Amritsar, Punjab,
winter and spring season so the analysis has been done INDIA; 3Department of Human Genetics, Guru Nanak Dev
University-143005, Amritsar, INDIA, Email: singh.sandip87@
from October 2017 to March 2018. The physico-
gmail.com; avnagpal@rediffmail.com
chemical characteristics of effluents shows a
deteriorating trend in the quality of water occur mainly Rivers are one of the most important factors for
in water bodies of industrial areas of Balrampur city. development of human civilization considering their use
for drinking water, irrigation, fisheries, aquaculture,
Key words: Balrampur, Chini Mill, BCM Ltd., Industrial areas.
transport and tourism. But, anthropogenic activities,
SVI/P-40 especially industrialization, urbanization and modern
agriculture have deteriorated the water quality of rivers
Characterisation of Groundwater around the world including India. The physico-chemical
parameters of river water are very important indicators
Quality in the Aquifer of Indo-Nepal of their quality and also help in understanding the
Border of Balrampur City sources of pollution. Therefore, a review of studies
published in last decade was done to summarize the
Zaheen Hasan* and D.D. Tewari physico-chemical characteristics of rivers from
Water Analysis Laboratory, Department of Botany, M.L.K. different states of India in order to assess their water
(P.G.) College, Balrampur, INDIA, Email: zaheenhasan666 quality. Reports from different states suggested that
@gmail.com rivers (such as Ganga, Yamuna, Sutlej, Mahanadi etc.)
and their tributaries are mostly uncontaminated or very
Present investigation deals about the groundwater
less contaminated in the states of their origin. But when
quality occur at aquifers found at thefoothills of Shivalik
these rivers enter the states having industrial and urban
at Indo-Nepal border of Balrampur. Here the water
development (such as Punjab, Uttar Pradesh, West
samples were collected from those areas where
Bengal, Maharashtra, Tamil Nadu, etc.) they get highly
industrial and municipal waste water leachate and
polluted due to anthropogenic activities such as
mixed with the groundwater. These ground waters are
discharge of untreated industrial effluents and urban
used as potable water for drinking and other purposes
sewage water, runoff from adjoining agricultural fields,
by humans. The analysis has been done especially
domestic activities such as washing of clothes, bathing
during summer and winter season mainly. In this season
of humans and animals in water, washing of utensils
the Groundwater quality is seriously affected by various
etc. The water quality of some rivers such as Yamuna
types of pollutants which are discharged from industrial
and Sutlej has been deteriorated to extremely low levels

164 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

in downstream states like Delhi and Punjab, SVI/P-43

respectively. Hence, adequate steps must be taken in
the downstream states to ensure that anthropogenic Contamination of Ground Water Due
activities must not pollute the rivers. to Leakage of Septic Tanks
Key words: Industrialization, Physico-chemical
characteristics, Rivers, States of India, Urbanization. Lisha Taneja and Mayank*
Amity School of Earth and Environmental Sciences, Amity
SVI/P-42 University Haryana, Gurugram-122413, Haryana, INDIA,
Email: lishataneja@gmail.com, mayank301095@gmail.com
Periodic Dissemination of Phthalate
Onsite disposal of waste water used by offices
Esters in Pond Water of the Amritsar and household which is not connected to city disposal
City, Punjab, India system is of great concern. However, the improperly
designed, constructed or ill maintained septic tanks are
Sneh Rajput, Rajinder Kaur and Saroj Arora of more concern as water percolates in the soil and
Department of Botanical and Environmental Sciences, Guru reaches ground water and contaminates it. There are
Nanak Dev University, Amritsar-143005, INDIA, Email: circumstantial evidences of septic tanks in some rural
snehrajput89@gmail.com areas where bottom is not cemented therefore;
wastewater has direct contact with the soil. The low
Phthalates are the dialkyl or alkyl aryl esters of
cost septic tanks, especially in the rural areas under
phthalic acid and used in PVC to provide softness and
Swatchh Bharat Mission programme, are often
flexibility. Phthalates have been identified as a priority
constructed without proper lining at the bottom to further
pollutant by The United States Environmental
reduce on the cost. Wastewater seeps through the
Protection Agency. The present study was planned to
layers of soil and reaches the ground water making
envisage the seasonal dissemination and contamination
ground water unfit for useful purposes like irrigation,
of five phthalic acid ester in pond water of Amritsar,
and drinking. The large scale use of such incomplete
Punjab (India). The water samples were collected for
septic tanks have a long term effect on the groundwater
a period of two years for four different seasons at eleven
of the region, which is evident through quality of the
different sites. The highest phthalic acid esters
water drawn through pumps. The ground water
contamination was found in the samples collected during
contamination by sewage may lead to entrance of urea
the monsoon season (0.291-2.787 ppm) followed by
in body as well as heavy isotope of nitrogen will enter
post-monsoon season (0.291-1.776 ppm) and winter
into food chain.
season (0.326-1.684 ppm) of the first year of sampling.
Benzyl butyl phthalate was the most prominent Key words: Sewage, Groundwater pollution, Low cost septic
phthalate found in 31.81% samples followed by di-n- tanks, Swatchh Bharat Mission.
butyl phthalate and dimethyl phthalate. Diallyl phthalate
and diethyl phthalate was not detected in any water SVI/P-44
sample. Seasonal distribution of phthalates in water
varied with the sampling site, the surrounding aquatic
Study of Physico-Chemical Analysis of
environment and local atmospheric deposition. Groundwater for Pre- and Post-
Temperature and precipitation were identified as the Monsoon Variation of Gorakhpur
two main governing factors for the seasonal variability
of phthalates concentration in water. The study offers District, Uttar Pradesh, India
the first set of data on the distribution of phthalic acid
Priyanka Chaudhary
esters in pond water of Amritsar district of Punjab
(India). The results confirm the presence of phthalic Department of Botany, Deen Dayal Upadhayay Gorakhpuur
acid esters in the water body. University, Gorakhpur, U.P. INDIA, Email: priyanka.
Key words: Pond water samples, Phthalic acid, Benzyl butyl
phthalate, Di-n-butyl phthalate, Dimethyl phthalate. Present study deals for the analysis of physic-

Abstracts 27-30 November, 2018, NBRI, Lucknow 165

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

chemical parameters of groundwater for pre and post cytological analysis. The cytogenotoxicity of fertilizers
monsoon variations in Gorakhpur district. For the study was assessed by markers- mitotic index and
of groundwater total twenty samples are collected from chromosomal aberrations during the different stages
different sampling sites during two season winter (post of mitosis. Results will be discussed.
monsoon ) and summer (pre monsoon). The physic- Key words: Biomarkers, Cytogenotoxicity, Allium cepa,
chemical parameters like temperature, pH, EC, TDS, Genotoxic.
Total hardness, turbidity, chloride, alkalinity, fluoride,
nitrate were analyzed. It has been found that the value SVI/P-46
of parameters is higher in pre monsoon season than
post monsoon season. The purpose of this study is to Pyrenocarpous Lichens as an
verify the quality of ground water. Indicator of Ecological Continuity in
Key words: Ground water, Total Hardness, Pre monsoon, Forests of Manipur and Nagaland
Post monsoon, Sampling sites.

SVI/P-45 Komal Kumar Ingle*, Sanjeeva Nayaka,

Vindhyeshwari Uppadhyay and D.K. Upreti
Biomarkers based Comparative Lichenology Lboratory. Plant Diversity, Systematics and
Cytogenotoxicity Assessment of Two Herbarium Division, CSIR-National Botanical Research
Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh - 226001,
Fertilizers INDIA, Email: ingle.komal@gmail.com
The pyrenocarpous lichens are characterized by
Sonam Verma* and Alka Srivastava
a pitcher like ascomata, carbonized laterally with the
In Vitro Culture and Plant Genetics Unit, Department of involvement of substrate bark cells, completely
Botany, University of Lucknow, Lucknow-226007, INDIA, immersed in the thallus or covered at the base, opening
Email: sonamv529@gmail.com, alkasrivastava@hotmail.com by a pore at the top. Most of the Pyrenocarpous lichens
These days use of fertilizers has become a regular comprise of green alga particularly trentepohloid while
farming practice due to the food crisis emerging as a few species have blue-green alga. During a recent
consequence of population growth and subsequent survey in Manipur and Nagaland state, a total of 87
need of augmenting in the agriculture production. Within localities of 5 districts were surveyed, and more than
the existing agriculture system, agrochemicals are 1,800 lichen specimens were collected. All the
classified as one of the major chemical pollutants that specimens identified and resulted in 26 species of
are disseminated all over the planet. However, there is pyrenocarpous lichens. The pyrenocarpous lichens are
still little information about the effects of fertilizers on widely distributed in the study area as the species were
producers that occupy the first levels of high sensitivity encountered in almost all the localities surveyed. Some
in the trophic chain. The use of biomarkers in the of pyrenocarpous lichens are substratum specific as
evaluation of toxic effects of agrochemicals has found growing exclusively either on trees or rocks. The
increased significantly in the last decade. Biomarkers species of Verrucaria, mostly found growing on rocks
are sensitive and quick indicators of the presence of while members of Pyrenulaceae and Trypetheliaceae
pollutants in the biomonitoring experiments. The present prefers to grow on the bark. The moist evergreen forest
study deals with comparative assessment of having smooth and moist bark mostly exhibit the rich
cytogenotoxic effects of two commonly used fertilizers: diversity of Pyrenocarpous lichens as species of lichen
urea and ammonium nitrate by using biomarkers viz. genera Anthracothecium, Lithothelium and Pyrenula
cytotoxic and genotoxic markers in the test plant Allium prefers to grow on smooth bark. The species of genus
cepa L. For this, pot experiments were set up, in which Anisomeridium were found growing on hard, rough
both fertilizers were added separately to the sample and dry barked tree trunk. The distribution pattern of
soils and healthy onion bulbs were planted at varying Pyrenocarpous lichens clearly grouped the forests into
day/s after treatment (DAT). Roots from onion bulbs distinct zones with evergreen, semi-evergreen and dry
grown in these were fixed at 3rd day after planting for deciduous types of forests. The Mt. Saramati area

166 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

located in the Nagaland having old growth trees exhibit SVI/P-48

the rich diversity of pyrenocapous lichen than the
disturbed, thin out forest near human settlements. A Consortium of Alga (Chlorella
Key words: Lichenized fungi, Pyrenocarpous, Forest types, vulgaris) and Bacterium (Pseudomonas
North-East. putida) for Amelioration of Arsenic
SVI/P-47 Toxicity in Rice: A Promising and
Feasible Approach
Silicon and Selenium Reduce Arsenic
Uptake and Mitigate Arsenic Toxicity Surabhi Awasthi1, Reshu Chauhan1, Suchi Srivastava1,
in Rice (Oryzasativa) Plants by Sudhakar Srivastava2 and R.D. Tripathi1
CSIR-National Botanical Research Institute, Rana Pratap
Activation of Antioxidant Enzyme Marg, Lucknow-226001, INDIA; 2Institute of Environment
System & Sustainable Development, Banaras Hindu University,
Varanasi-221005, INDIA, Email: surabhi.microbiology@
Amit Kumar 1,2 *, Sanjay Dwivedi 2 and R. D. gmail.com
Tripathi2 In the present study, arsenic (As) toxicity
Department of Botany, University of Lucknow, Lucknow- amelioration potential of a consortium of plant growth
226007, INDIA; 2Plant Ecology and Environmental Science promoting rhizobacterium (Pseudomonas putida) and
Division, CSIR-National Botanical Research Institute, alga (Chlorella vulgaris) was evaluated during
Lucknow-226001, INDIA, Email: amit_gene@yahoo.com, arsenate (AsV) exposure to rice (Oryza sativa) plants
amit.metagene@gmail.com for 15 d. The consortium mediated amelioration of As
Arsenic (As) is toxic to plants and animals, toxicity was evident through improved growth of rice
making it necessary to develop strategies that seek to plants (root and shoot length and biomass) and reduced
reduce its introduction into food chains. Thus, the aim oxidative stress [as level of superoxide radicals (O2"),
of this study was to investigate whether silicon (Si) hydrogen peroxide (H2O2 ) and membrane damage].
and selenium (Se) reduce As concentrations in rice. A The positive responses were attributable to a significant
comprehensive study was conducted with tolerant decline in As accumulation in root (94 mg kg-1 dw) and
variety of rice (BRG-12) in different combinations of shoot (51 mg kg-1 dw) in consortium (P. putida + C.
As, Si and Se under hydroponic condition. Results vulgaris) inoculated seedlings as compared to As alone
showed that As treated rice plant with the combination exposed plants (156 and 98mgkg”1dw, respectively).
of Si and Se significantly reduces the As accumulation There were also significant changes in the level of
in comparison to other treated plants. The diminish As various nutrient elements (Mn, Fe, Co, Zn, Mo and
accumulation by 64% in comparison to control in shoot Cu), thiols and in the activities of antioxidant and thiol
clearly reflects a protective and combined positive metabolism enzymes in the consortium inoculated
effect of Si and Se on As toxicity. Increased growth seedlings that allowed the plants to tolerate As stress
responses, enzymatic antioxidant system (SOD, APX, effectively and achieve better growth. The study
GPX, GR, GST and POD) signify tolerance responses demonstrated that consortium of P. putida and C.
in As treated rice. Results demonstrated that vulgaris may alleviate As stress and improve growth
supplemented with combination of Si and Se effective of rice seedlings along with reduction in As levels.
limit As translocation, tolerance and increase growth Key words: Arsenic, Chlorella vulgaris, Pseudomonas
responses in rice which could be used as a supplements putida, Growth, Rice.
in the As affected paddy field. However, field trial must
be imperative prior to application in agriculture land.
Key words: Antioxidants, Arsenic, Nutrients, Rice, Silica,

Abstracts 27-30 November, 2018, NBRI, Lucknow 167

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVI/P-49 SVI/P-50

Diminution of Arsenic Accumulation in Evaluation of Phytoremedial Potential

Rice Seedlings Co-Cultured With of Pteris vittata through Cytogenetic
Anabaena Sp.: Modulation in the Biomarker using Vicia faba
Expression of Lower Silicon Transporters,
Kiran Gupta*, Alka Srivastava and Amit Kumar
Two Nitrogen Dependent Genes and
Plant Genetic Unit, Department of Botany, University of
Lowering of Antioxidants Activity Lucknow, Lucknow-226007, INDIA, Email: sunrays79@gmail.
Ruma Ranjan1,2*, Navin Kumar 1, Arvind Kumar
A study was carried out to assess the
Dubey 1 , Ambedkar Gautam 1 , Shyam Narain
phytoremedial potential of Pteris vittata by employing
Pandey2 and Shekhar Mallick1 *
Vicia faba L. test system. In that context comparative
CSIR-National Botanical Research Institute, Lucknow- study of cyto-genotoxicity in Vicia faba was done in
226001, INDIA; 2University of Lucknow, Lucknow-226007, untreated and Pteris vittata treated As affected soil
INDIA, Email: connect.ruma89@gmail.com samples. The roots of Vicia faba L. were exposed to
The present study was intended to investigate the Pteris vittata treated and untreated As affected soil
role of algae, Anabaena sp. in the amelioration of As samples. The roots of V. faba analyzed in terms of
toxicity, when co-cultured with rice seedlings. The mitotic activity, chromosomal aberration as well as
reduction of growth in rice seedlings against As(III) micronuclei end point. Study revealed that inhibition of
and As(V) was recovered with Anabaena sp. The mitotic index and stimulation of mitotic depression;
Anabaena sp. also reduced the accumulation of As, chromosomal aberrations and micronuclei were more
where it was more efficient against 60 ìM As(III) in roots of V. faba exposed to untreated soil samples in
(49%) than As(V) (23%) in rice shoot. Similarly, with comparison to Pteris vittata treated As affected soil
reduction of As accumulation, lower silicon transporters samples. These cytogenetic biomarker like
(Lsi-1 and Lsi-2) was found to be suppressed against enhancement of mitotic index and decrease in mitotic
As treatments. However, the expression of two nitrogen depression ,chromosomal aberrations as well as
dependent genes i.e., NR and SAMT were found to micronuclei endpoint in roots of V. faba exposed to
be enhanced with the Anabaena sp. Likewise, the treated soil samples Vicia faba revealed the
activity of antioxidant enzyme, GST, was enhanced, phytoremediation potential of Pteris vittata. Therefore
whereas, the activity of other enzymes such as SOD, it can be depicted on the basis of result that Pteris
APX, GPX, GR and DHAR were decreased with vittata has good phytoremediation potential for Arsenic
As+Algae combinations. Overall, the result suggested affected pollution.
that the Anabaena sp. reduces As accumulation, Key words: Biomarker, Vicia faba, Pteris vittata, Arsenic,
modulates gene expressions and antioxidants to Chromosomal aberrations, Mitotic depression.
ameliorate the As toxicity in Oryza sativa L.
Keywords: Arsenic, Anabaena sp., Lower silicon transporter, SVI/P-51
S-adenosylmethionine-dependent methyltransferases, Oryza
sativa L. In-vitro Degradation of Fluorene by
Bacteria Mediated by Catabolic
Nitanshi Jauhari and S.N. Singh
Plant Ecology and Environmental Science Division, CSIR-
National Botanical Research Institute Lucknow, Uttar
Pradesh, INDIA, Email: nitanshijauhari27@gmail.com

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Polycyclic aromatic hydrocarbons (PAHs) are cause pathological stress, false satiation, reproductive
ubiquitous environmental pollutants found in soil and complications, blocked enzyme production, reduced
sediments, groundwater and atmosphere. Many PAHs growth rate and oxidative stress in organisms. MPs
and their epoxides are toxic and mutagenic / can also potentially transfer chemicals adsorbed on their
carcinogenic in nature. This investigation evaluates the surface in the food chain. Currently, knowledge and
degradation ability of three bacterial strains, such as data on the accumulation and effects of microplastics
Pseudomonas aeruginosa PSA5, Rhodococcus sp. in freshwater and terrestrial systems is much limited
NJ2 and Cronobacter sp. PSM10, isolated from than that in marine systems. In this context the
petroleum sludge containing 500 ppm of fluorine in occurrence of microplastic pollution was investigated
mineral salt medium. A maximum degradation of 79% in the sediment and the mussel samples from the Ganga
was attained by PSA5, followed by PSM10 (71%) and river at twelve sites in Varanasi. The samples were
a minimum was recorded for NJ2 (68%) after 7 days processed and measured for microplastic through alkali
of incubation. Induction of catabolic enzymes of two digestion and density separation. Results suggest the
major pathways involved in the formation of catechol presence of variety of microplastics including fibres
and protocatechuate as intermediates was also and fragments in the sediments as well as tissues of
examined. Between the two pathways, enzymes mussels. Based on our results, it is evident that
associated with protocatechuate pathway were more microplastic pollution in River Ganga at Varanasi is
induced than that of catechol pathway. Besides, statistically significant and that freshwater mussels due
degradation kinetics and half life of anthracene were to their extensive filter-feeding activity are exposed to
also determined to find out the degradation pattern these microplastics directly present in the water column
caused by bacteria. Biosurfactant production, cell which may thereby enter our food chain, however
surface hydrophobicity and emulsification index of detailed studies are needed to prove the hypothesis.
anthracene, which facilitate anthracene degradation, Key words: Fresh water pollution, Food chain, Ganga river,
were also determined in all bacteria to substantiate their Microplastics, Varanasi.
degradation potential.
Key words: Fluorene contamination, Anthracene, Catabolic, SVI/P-53
Degrading microbe.
Fluoride: Pollution and Mitigation
Gayatri Singh 1 *, Geetgovind Sinam 1 , Babita
Microplastic Pollution in River Ganges Kumari1 , Kriti1 , Mahiya Kulsoom2 and Shekhar
at Varanasi: A Case Study Mallick1
Plant Ecology and Climate Change Science Division, CSIR-
Vyomendra Chaturvedi* and Kavita Shah National Botanical Research Institute, Lucknow-226001, U.P.,
INDIA; 2Department of Environment Science, Babasaheb
Environmental Biotechnology Laboratory, Institute of Bhimrao Ambedkar University, Lucknow-226025, INDIA,
Environment and Sustainable Development, Banaras Hindu Email: gayatrisingh.15@gmail.com
University, Varanasi-221005, INDIA, Email: vyomendra.
chaturvedi04@bhu.ac.in, kavitashah@bhu.ac.in Fluorosis is the most prevalent groundwater-
related disease in India, the most severely affected
Microplastics (MPs), a manmade litter having
country worldwide. The desirable and the maximum
particle size <5 mm, have recently been identified as
permissible limits of the flouride concentrations in the
an emerging global concern which affects aquatic
drinking water are 0.6 mg l-1 and 1.0 mg l-1, respectively
organisms and human beings. The small sizes of MPs
(WHO). In Uttar Pradesh Unnao is one of the most
make them easily available for ingestion by a wide
fluorosis affected district. Few studies have previously
variety of organisms such as zooplanktons, bivalves,
reported the F- level in the drinking water in Unnao
mussels, shrimps, oysters, copepods, worms and by
region, which ranges between 0.8-13.9 mg L-1. There
organisms from higher trophic levels as fish, marine
are various physical, chemical and biological methods
mammals etc. Ingestion of these tiny particles can

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

mentioned to defluoridation of drinking water. To Natural wetland act as a biofilter, removing sediments
mitigate this problem, there is an urgent need to and pollutants such as heavy metal from water, and
understand the current status and brief knowledge of F-. constructed wetland is designed to emulate these
Key words: Fluoride, Unnao, Defluoridation. featureas. Constructed wetland is built by human.
These constructed wetlands are primarily built to clean
SVI/P-54 up waste water and create wild life habitat. Constructed
wetland naturally filters and treats waste water and
Ganga Water Pollution Management are often less expensive than traditional treatment
by Green Clean Technology and
Key words: Ganga, Water pollution, Green technology.
Outreach Programmes
Mridul Kumar Shukla
Outreach Programmes, CSIR-National Botanical Research Estimation of Arsenic in Rice Grains
Institute, Rana Pratap Marg, Lucknow-226001, INDIA, Email:
mridul_shukla@rediffmail.com; mridulcsir0101@gmail.com
and Rice-Based Food Products in
Gangetic Region
The Ganga river has been considered as the most
sacred river of India in Ved Puran. It is called as “Ganga Nidhi Tyagi and Sudhakar Srivastava*
Maa” or Mother Ganga or Ganga Ji. People of world
believe that a bath in the holy water of Ganga washes Institute of Environment and Sustainable Development,
all the past sins of a person. It has a symbol of purity, Banaras Hindu University, Varanasi-221005, INDIA, Email:
sudhakar.iesd@bhu.ac.in, sudhakar.srivastava@ gmail.com
and for Mokshh . It has long history of reverence in
India and it is often called Holy Ganga. The total length Arsenic (As) is a toxic carcinogenic element
of the river is about 2510 K.M.; one of the largest which poses threat to millions of lives around the globe.
groups of human population belts on earth is built around The element has already entered the food web as many
the Ganga. Its originated from Bhagirathi from Gangotri of the crops and animal products are reported to have
glacier in the Uttarakhand. The basin drains eight As in high concentrations. The range of health impact
states, H.P., Panjab, Haryana, U. P., Rajasthan, M. P., of As is from skin disorder to cancer. In few reports,
Bihar, West Bengal and Delhi. The level of pollution in As exposure has been linked to diabetes mellitus and
the Ganga River has reached alarming proportions. Two adverse pregnancy outcomes (APO) too. The present
types of point sources like, surface drains carrying study was conducted to analyze As content in rice
municipal sewage or industrial effluent, pumping stations grains of market sold rice varieties and rice-based food
and sewage system from industries etc are found. The products (laiyya, chuda, and snacks) in Gangetic region.
point source can be measured. Second is non point The cities covered in Gangetic region include Kanpur,
source which cannot be measured such as runoff Allahabad, Varanasi, Balia, Patna and Kolkata. In
agriculture field carrying chemical and fertilizers, run addition, Jabalpur was taken as a control area which is
off from area used for dumping of solid waste and not reported to have As contamination. The samples
open defecation, mass bathing, floral offerings etc. were oven dried, crushed and digested in nitric acid.
According to study nearly 89 million liter of sewage is After this, the samples were analyzed for As using
disposed into Ganga from 12 municipal towns that fall Atomic Absorption Spectroscopy. The results show
along its route till Haridwar, where the amount of presence of As in the samples that varied from variety
sewage disposed in the river increase during the Char to variety, and in different areas. The results are
Dham Yatra season. A constructed wetland is an discussed with possible health impacts considering
artificial marsh or swamp, created for anthropogenic standard As limits in rice grains, and in food products,
discharge such as waste water storm water runoff or rice consumption rates and As consumption limits for
sewage treatment and as a habitat for wild life, or for humans.
land reclamation after mining or other disturbances. Key words: Arsenic, Rice, Gangetic region.

170 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVI/P-56 Key words: Diesel, Petroleum hydrocarbon, Degradation,

Microbial consortium.
Degradation of Diesel using Co-
Culture of Bacterial and Fungal Strains
Interaction of Microalgae with
Babita Kumari*, Kriti, Mahiya Kulshoom, Gayatri
Singh, Shekhar Mallick and Pankaj Kumar Engineered Nano-Materials: Possibilities
Srivastava for Bioremediation and Biofuel
Environmental Technologies, CSIR-National Botanical Production
Research Institute, Rana Pratap Marg, Lucknow-226001,
INDIA, Email: babita.bit86@gmail.com Mohit Singh Rana1,2 , Shashi Bhushan1, Ruben
Diesel is one of fractionate part of crude Sudhakar2 and Sanjeev Kumar Prajapati1 *
petroleum oil that is a major source of energy for public 1
Bio-Resource Engineering Lab (BREL), Department of
and good transports. The use of these petroleum oils Chemical and Biochemical Engineering, Indian Institute of
can have negative impact on Earth’s biosphere by Technology (IIT) Patna, Bihta, Patna-801106, Bihar, INDIA;
contributing pollutants and greenhouse gases into the Department of Energy and Environment, National Institute
atmosphere, hence damaging not only ecosystems but of Technology (NIT) Trichy, Tiruchirappalli-620015, Tamil
also human health viz lung and bladder cancer. It is Nadu, INDIA, Email: mohitrn90@gmail.com,
composed of 75% of saturated and 25% of aromatic sanjukec@iitp.ac.in; sanjukec@gmial.com
hydrocarbons. Bioremediation agents like Engineered nanoparticles (ENPs) are emerging
heterogeneous bacteria and fungi are being substances, being widely used in the biomedical
successfully applied to degrade petroleum hydrocarbon industries, disinfectant, process engineering, surface
because they completely mineralized it into CO2 and coating, cosmetics and food packaging. By the virtue
H2O or transformed it into non-toxic metabolites. In of the Advanced Oxidation Technology (AOT),
the study, diesel degrading efficiency of potent inherited by nanoparticles, these materials generate
petroleum hydrocarbon degrading bacterial and fungal highly reactive transitory species (i.e. H2O2, OH·, O2·-
strain i.e., Pseudomonas sp. BP10 and Penicillium , O3) and mineralize various organic and inorganic
sp. PS10, respectively was studied as individual or in molecules. Further, some selected ENPsshow positive
combination. Co-culture of these strains has been found effect on microalgal growth, biochemical accumulation
capable to degrade 65% of diesel (5%) in minimum and biofuel yield. Hence, at the optimal concentration,
salt media after 15 days of incubation period. the nano-materials can be exploited for bioremediation
Individually, only 55% and 40% degradation was along with microalgae, leading to green sustainable
recorded by bacterial and fungal strain, respectively. technology. In the present work, effect of Fe2O3 ENPs
Compared to fungal strain, bacterial strain was found supplementation on growth and composition of
more potent in degradation of diesel. Specific activity Chlorella spp. was tested. Interestingly, the Fe2 O3
of degradative enzymes i.e., alkane dehydrogenase and ENPs at a concentration of 20-30 mg L-1 showed up
laccase and induction of biosurfactant (glycolipid) were to 40 % increase in biomass concentration during 15
also found higher for bacterial strain. Bioaugmentation days batch cultivation. Additionally, the biochemical
of co-culture of these bacterial and fungal strains composition was also improved. Further, pretreatment
showed synergistic relation and induce 50 and 200% of the microalgal cells was confirmed through visual
of specific activity of degradative enzymes and observations, when the ENP containing biomass was
induction of biosurfactant, respectively. Consequently, incubated under direct sun light (for Fenton reaction)
the rate of degradation (65%) of diesel exaggerated and under UV light. Hence, the pretreated biomass,
owing co-culture of potent microbial strains. So, this having improved biochemical composition, may result
study recommends the use of bacterial and fungal strain in higher biofuel yield as evident from previous reports.
consortium for the degradation of diesel hydrocarbons However, practical biofuel yield needs to be determined
compounds to decontaminate the polluted sites. to assess the feasibility of the process. In future, the

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

ENPs may also be tested with microalgae in municipal characterized for different plant growth promoting
wastewater, to assess the effect on biomass yield as attributes viz. indole-3-acetic acid, phosphate
well as pollutant removal. If successful, the proposed solubilization, siderophore production. Assessment of
process will serves as a role model for bioremediation arsenic stress amelioration using rice as a host plant
coupled biofuel production using microalgae. under green house condition is under progress.
Key words: Engineered nano-materials, Biofuel, Key words: Arsenic, Yeast, Arsenite oxidase, Reductase
Bioremediation, Microalgae, Wastewater. activity.

SVI/P-58 SVI/P-59

Characterization of Yeast Strains with The Role of Metallothione in Gene in

Potential of Arsenic Stress Ameliora- Abiotic Stress
Sanoj Kumar*, Arvind Kumar Dubey and
Jasvinder Kaur, Vandana Anand, Sonal Srivastava, Indraneel Sanyal
Pankaj Kumar Srivastava and Suchi Srivastava* Plant Transgenic Laboratory, Molecular Biology and
Biotechnology Division, CSIR-National Botanical Research
Division of Microbial Technologies, CSIR-National Botanical
Institute, Lucknow-226001, INDIA, Email: sanojyadav152
Research Institute, Lucknow-226001, INDIA, Email:
Metallothionein (MT) is a ubiquitous low
Arsenic is the most common toxic substance in
molecular weight protein ranging from 5-14 kDa, with
the environment, ranking first in the inorganic toxicants.
high cysteine content and has strong affinity for heavy
Its distribution in environment causes serious human
metals. It was first isolated from equine kidney. The
health issues worldwide due to its highly carcinogenic
metallothionein (MT) are small, cysteine-rich heavy
properties. Microbes play a significant role in
metal-binding proteins which participate in an array of
detoxification of this metalloid. Microbial activity is
protective stress responses. Based on the arrangement
linked to the biogeochemistry of arsenic and is involved
of Cys residues, plant MTs are classified into type-I,
in its biotransformation. Many bacteria, archaea, and
II, III and IV. MT provides protection against heavy
fungi have developed different mechanisms to prevent
metal toxicity, oxidative stress, and participates in the
themselves from arsenic stress. These mechanisms
regulation of physiological metals like zinc (Zn2+) and
include the reduction of arsenate to arsenite; oxidation
copper (Cu). The level of the response to these
of arsenite to the arsenate; immobilization, complexation,
inducers depends on the MT gene. These activities
vacuolar sequestration and its methylation to less toxic
are regulated through intracellular metal ion modulation
organic form. In this study we have isolated yeasts
and free radical scavenging. MTs transcript levels in
from rhizosphereric soil of different regions of India.
various plants alter during abiotic stress caused by salt,
Three strains from Bulandshahr and Shillong has been
heat, cold, heavy metals contaminations etc. MTs are
selected as best As tolerant saccharo-type yeast strains.
involved in maintaining homeostasis of essential metals
The present study deals with the identification,
and metal detoxification and implicated in a range of
characterization and detoxification mechanisms in
other physiological processes. We are presently
selected yeast strains. Selected yeast strains with
working on chickpea exploring the role of MT under
arsenite oxidase and arsenate reductase activities were
drought stress, using molecular approaches.
identified as Candida tropicalis (NBRIB3.4),
(NBRIB3.5) and Debaryomyces hansenii Key words: Metallothionein, Abiotic stress, Cysteine, Heavy
(NBRISh2.11). In addition these strains were also metal.

172 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVI/P-60 SVI/P-61

Remediation of Heavy Metal Identification and Characterization of

Contaminated Soils: An Innovative Multi-trait Quorum Sensing Exhibiting
Technology for the Present and Future PGPR Isolates and Assessing their
Research Prospective as Inoculants for Zea
Khusboo Pandey and K.K. Tiwari
Sophisticated Instrumentation Centre for Applied Research Arpita Singh* and Puneet Singh Chauhan
& Testing (SICART), Vallabh Vidyanagar, Anand-388120,
Microbial Technologies Group, CSIR-National Botanical
Gujarat, INDIA, Email: pandeykhushboo94@yahoo.com
Research Institute, Lucknow-226001, INDIA
Contamination of lands with toxic heavy metals Email: puneetnbri@gmail.com, puneet@nbri.res.in
is a widespread environmental issue resulting from
global industrialization. Heavy metal contaminants are Development of an effective plant growth
a serious problem worldwide to human health, animals promoting rhizobacteria (PGPR) inoculant necessitates
and some plants. Conventional techniques for the presence of a diverse set of traits that can help its
reclamation of such soils are expensive and colonization of the rhizosphere and survival under
environmental non-friendly. Phytoremediation is an varying environmental conditions. In our investigation,
emerging group of technologies utilizing green plants a set of 310 bacterial isolates from extreme
to clean up the environment from contaminants and environment and Zea mays rhizosphere of plants were
has been offered as a cost-effective and non-invasive screened initially on the basis of acyl homoserine lactone
alternative to the conventional engineering-based (AHL) production, plant growth promoting (PGP)
remediation methods. Biochemical mechanisms such attributes like indole acetic acid (IAA) production,
as adsorption, transport, accumulation and translocation Phosphate solubilization, siderophore production, abiotic
that are adopted by plants are of supreme importance stress tolerance namely drought (30%, 45% and 60%
in developing improved phytoremediation. Identification Poly Ethylene Glycol 6000), salt (8% and 12% NaCl),
of suitable hyperaccumulating plants with remarkable pH (pH5, pH9 and pH11) and temperature (37°C and
biochemical mechanism, presents a fascinating success 45°C) and antagonistic activity. The screening led to
in the field of phytoremediation as well as other the selection of 13 isolates (12VPNC2, PC8, PD4,
research area. There are different versions of 7VP51.8, P51.9, 11VPKHP4, P51.8, P51.10, NBRI
phytoremediation viz. phytoimmobilization, N7, NBRI N11, NBRI N12, NBRI N15, NBRI N21),
phytostabilization, rhizofilteration, phytovolatilization and proficient in AHL production, withstanding different
phytoextraction, the latter being most widely accepted abiotic stress conditions as drought (30%, 45% and
for remediation of soils contaminated with toxic heavy 60% Poly Ethylene Glycol 6000), salt (8% and 12%
metals. Phytoremediation includes phytovolatilization, NaCl), pH (pH5, pH9 and pH11) and temperature (37°C
phytostabilization, and phytoextraction using and 45°C), exhibiting plant growth promoting attributes
hyperaccumulator species or a chelate-enhancement along with biofilm formation, exopolysaccharide (EPS)
strategy. Different aspects of phytoextraction and production, alginate production, aminocyclopropane-1-
scope of this technology for remediation of heavy metal- carboxylic acid (ACC) deaminase activity and
contaminated soils are reviewed discussing challenges antagonistic activity and are also proficient in forbearing
and opportunities in realizing phytoextraction as an with heavy metal contamination. The plant growth
economically viable remediation method. promotion potential of selected thirteen bacterial strains
showed significantly growth enhancement in all the
Key words: Phytoremediation, Heavy metals, Rhizosphere
parameters of Zea mays.
community, Genetic engineering.
Key words: Quorum sensing, PGPR, Zea mays

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Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVI/P-62 microbes, (2) Determining the effects of metal stress

on growth of sunflower and microbes present in the
Impact of Pyrometallurgical Slags on rhizosphere, (3) Developing phytoremediation and
Sunflower Growth, Metal Accumula- phytomanagement strategies for reclamation of
affected soils and focusing on safe disposal strategies
tion and Rhizosphere Microbial for slag. Present work was designed to determine the
Communities release of metals from slag, leaching test was
performed on granulated slags(GS), in presence of soil
Priya Jaiswal*, Ana Carolina Agnello and Puneet pre solution, which showed us that soil pore solution
S. Chauhan increases release of Copper from GS. Sunflower
Microbial Technologies Group, CSIR-National Botanical (Helianthus annus) was used for investigating metal
Research Institute, Lucknow, 226 001 accumulation. Sunflower was grown in a 50% w/w
substrate(mix of agricultural soil and GS) and it was
Email: puneetnbri@gmail.com, puneet@nbri.res.in
able to accumulate metals in above ground tissues.
Slag, a by-product of metallurgic processes is Community level physiological profiling (CLPP) and
dumped into the environment as such ignoring the 16S rRNA gene based denaturing gradient gel
hazards caused by it on the flora and fauna. Several electrophoresis analyses was performed for determining
studies have confirmed the presence of heavy metal shift in rhizospheric and bulk soil microbial diversity
residues left in the slag which becomes a source of induced by presence of slag. Findings from this study
heavy metal deposition in the environment. Thus, human suggested that metallurgical wastes should be treated
intervention becomes imperative to resolve this before dumping in the environment to minimise the
environmental concern by utilising sustainable metal toxicity.
remediation methodologies. The main objectives of the Key words: Metal accumulation, Rhizosphere, Microbial
current study includes- (1) investigating release of diversity.
metals from slags by the influence of plants and

174 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Session VII
Plant Response to Environmental Pollution

Abstracts 27-30 November, 2018, NBRI, Lucknow 175

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

176 Abstracts 27-30 November, 2018, NBRI, Lucknow

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

SVII/L-1 adopted, exogenous applications of silicon (Si), salicylic

acid (SA) and arbuscular mycorrhizal (AM) propagules
Environmental Mercury Stress- have emerged as potent bio-tools for alleviating stress
Adaptation in Selected Macrophytes induced effects in crop plants. However, little is known
about the alleviative role of these amendments in
- Crosstalk on Chemical and Biological legumes, which vary in their sensitivity towards different
Mechanisms abiotic stresses. Studies were conducted in Cajanus
cajan (L.) Millsp. and Cicer arietinum (L.) where
Naser A. Anjum1,2 different mechanism(s) adopted by stress alleviants
Centre for Environmental and Marine Studies (CESAM) and were explored and identified through a series of
Department of Chemistry, University of Aveiro, 3810-193 experiments under stressful conditions. Si maintained
Aveiro, PORTUGAL; 2Present address: Department of plant growth by improving light perception, imparting
Botany, Faculty of Life Sciences, Aligarh Muslim University, hardiness to plants, reducing toxic ion uptake and
Aligarh-202002, U.P., INDIA, Email: dnaanjum@gmail.com, maintaining nodular efficiency under salinity and
g0216@myamu.ac.in metal(loid) stress. Exogenous SA positively regulated
Salt marsh macrophytes have been recognized membrane stability, nutrient uptake, and modulated
as the managers of pollution of varied types (including enzymes involved in carbohydrate metabolism thereby
chemicals) in the coastal areas. However, the major regulating reproductive potential. AM could impart
mechanisms underlying salt marsh macrophytes- abiotic stress tolerance by enhancing root water
adaptation to persistent chemical contaminants (such absorption capacity, improved nutrient status, modulated
as mercury, Hg) are least explored. This work is aimed proline and trehalose biosynthesis, and by sequestering
to present a critical cross-talk on the success and toxic ions in underground plant organs. When applied
failure of non-biological (chemical traits) and biological together, interactive effects were observed where
(physiological/biochemical, and polypeptide patterns) mycorrhization enhanced endogenous Si content in
mechanisms underlying salt marsh macrophytes otherwise low Si accumulating legumes that conferred
Juncus maritimus and Halimione portulacoides (root/ enhanced stress tolerance. Similarly, exogenous SA
rhizome/shoot) adaptive responses to Hg under improved root colonization ability of AM fungus, thus
environmental exposure. imparted functional complementarily by modulating
carbohydrate metabolism. In addition, effects observed
Key words: Chemical pollution, Mercury, Coastal lagoon, varied among differentially tolerant genotypes. Hence,
Salt marsh macrophyte, Stress adaptation
application of exogenous amendments along with
SVII/L-2 mycorrhiza could be used as a promising strategy in
achieving sustainable production of economically
Emerging Role of Silicon, Salicylic important legumes under abiotic stresses.
Acid and Arbuscular Mycorrhizal Key words: Abiotic stress, Arbuscular mycorrhizae, Legumes,
Tolerance mechanism(s), Silicon, Salicylic acid.
Fungi in Alleviating Abiotic Stress in
Legumes SVII/O-1

Neera Garg Nitric Oxide Exposure Impacts on

Department of Botany, Panjab University, Chandigarh- Morphology and Auxin Transport of
160014, INDIA, Email: gargneera@gmail.com, garg_neera@ Oryza sativa under Arsenic Stress
Soil salinization and heavy-metal contamination Afsana Praveen* and Meetu Gupta
are the major eco-environmental factors that hamper Ecotoxicogenomics Lab, Department of Biotechnology, Jamia
legume production worldwide by negatively influencing Millia Islamia, New Delhi, INDIA, Email: afsana735@gmail.
plant metabolism. Among the various approaches com, meetu_gpt@yahoo.com

Abstracts 27-30 November, 2018, NBRI, Lucknow 177

Sixth International Conference on Plants & Environmental Pollution (ICPEP-6)

Nitric oxide (NO), a signal molecule play vital agricultural areas has raised an alarming situation. Rice
role to provide tolerance to abiotic stress in plants by is an important food crop of India which feeds about
interplay with reactive oxygen species, and thus half of the population, but presence of As in
promoting their growth and development. Arsenic (As) environment has lead to its accumulation in edible plant
is a toxic metalloid and its contamination found in crop parts, which effects human and animal health. In this
plants, mainly rice which is an important diet for millions study, we have analyzed the effect of AsIII on two
of people. In this study 7 days old hydroponically grown rice varieties- Varsha and Jaya. Rice seeds were
rice seedlings were exposed to As(III) (150 µM), NO exposed to AsIII (150ìM) for 7 days to observe seed
(100 µM), As(III)+NO for 48 h and control (without germination and relative As-injury rate while
metal).We observed NO mediated alteration on hydroponically grown 12 days old seedlings were
physiological, biochemical and stress related exposed to AsIII (150ìM) for 96 h to study the changes
parameters along with auxin transporter PIN genes (Os at biochemical and molecular levels. Changes in
PIN1a, Os PIN1b, Os PIN1c, Os PIN1d, Os PIN2, phenotypic characters, physiological parameters
Os PIN5a, Os PIN5c, Os PIN8, Os PIN9, Os (chlorophyll, protein), stress indicators (MDA and
PIN10b), expression under As stress. As exposure H2O2) along with accumulation of As and macro/micro-
reduced the overall plant growth, formation of lateral nutrients (Na, Mg, S, Mn, Fe, Cu and Zn) constituted
roots, chlorophyll and protein content and enhanced the biochemical approaches, while RAPD analysis was
the oxidative stress by increasing the level of antioxidant done to observe the changes at molecular level. WRKY
enzymes (SOD, CAT, APX, GR), and stress related TFs play a key role in plants during heavy metal stress,
parameters (cysteine, proline, MDA, thus an attempt has been made in this study to observe
H2O2).Supplementation of NO along with As reduced their expression analysis in response to AsIII toxicity
the accumulation of As in rice seedling, improved plant in both rice varieties. Our observations showed that
growth, lateral root formation, increased chlorophyll and seed germination, phenotypic and physiological
protein content, diminished the level of antioxidant parameters decreased, while stress indicators increased
enzymes and stress related parameters by reducing during AsIII exposure as compared to control. Nutrient
the ROS generation. Addition of NO also up regulates profiling results also demonstrated the negative effect
the gene expression of auxin transporters. Overall, NO of AsIII on both rice varieties. Further, results obtained
reduced the toxicity of As using various mechanism from RAPD band profiles and GTS were compatible
and provi