‘re, BIOLOGICAL CONSULTING SERVICES. OF NORTH FLORIDA, INC: January 17,2019, caryn Benjamin Louisiana Department of Heath Baton Rogue LA 70821, 225-342-7499 Ccarynsenjamin@la gov (lent to: POE-001 1401 Hato, Raw 1401 Hamilton TCR.OS3 1322 Violet, TCR-089 8311 Lantana, TCRO8 5370 Bluebell {8s 10; 1801058, 1901058, 29¢1060, 1901061, 1901063 ear Caryn Benjamin, We have completed the analysis ofthe submitted samples 3s outed below, Project Name: City of Bossier LALO15004 (heats Method INaegleia Fowler (Clture/PCR) COC method BCS SOP A-1 (NOT S017025 & NOT TN! Accredited) Following, you wll ind our repor- onthe results ofthe analysis conducted on the referenced samples ‘Should you have any questions, pease do not hesitate to contact me. Sincerely, Bernd Md Sone Mull PH Laboratory Operations Manager ICS LABORATORIES, INC, — GAINESVILLE 4609 NWETH STREET. STE. GAINESVILLE: FLORIDA 32609 “Tet. (952) 377.9272, Fax. 52) 377-5630 Www wickoblosenvices. conhent: Lousiana Department of Heath nasi Noeoleria Fowl (Culture/PCR) Project Name: City of BosierLA1015000, Cent Sample: POE-001 1401 Hamiton Sample Date/Time:January 07,2029 4S BCS sample 0: 1901058, Sample Description: Rexeed 255 Utatver DateRecelved: January 08,2019 11:18 Amount submited: 200 L Solids: WA Amount Analyed: 3000 L Receipt Temperature: 19.0 deg Preserved: Ho ‘alyss tar: January Us, 219 10:89 ‘analysts top Vate: January 16, 2019 16:48, analyst: Katherine Sayer, Pho. Qualiier: Primary Value: © Visual presence of Amoeba Trophozotes (0 for absence/1.0for presence) Secondary Value 0 Nacgleria fowler ONA detection (0 for absence/'.Ofor presence) ‘Analysis Notes: Active amoets traphozoltes not detected visually fowler PCR was negative. N, fowler was not detected inte sample lent: Lousiana Department of Heath ‘nals: NoeglrioFower(Cltre/PCR) Project Name: City of BossierLA1015004 Cen Sample: Raw 1401 Harton Sample Date/Time:January 07,2019 9:43, ‘cS Sample 1D: 1901058 Sample Description: Rexeed 255 Utratter DateReceled: January, 2019 11:18 Amount Submitted:10 Solids: WA Amount Anaiped: §— 100 Recelpt Temperature: 19,0 deg Preserved: Ho Analysis Start: January 08, 2019 13:48 ‘Analysis Stop Date: January 26,2019. 16:5 analyst: Katherine Sayer, PhO. Qualifier: Primary Value: 110 Visual presence of Amoeba Trophozoites (0 for absence/1.0 for presence) Secondary Value 10 Naoglria fowleri DNA detection (0 for absence/.0 for presence) ‘Analysis Notes: Active amoeba traphoroits visually detected. fowler PCR was postive. N. fowler was detected inthe spe. Fagellate transformation not done due to matin interference. Ftp 5 so sn oN, so. 1006) BCS LABORATORIES, INC, ~ GAINESVILLE 4609 NWETH STREET STE-A, GAINESVILLE FLORIOA 32609 “Tex (952) 3779272, Fax 52) 377-5630 FLDOH sen2924, ISO/IEC 17025:2005 12422 (ANAS, PADEPH 6.03950, EPAW FLO1147lent Louisiana Department of Heath Analysis ‘naegleria Fower(Culture/PCR) Project Name: City of Bossier LA101500¢ ‘ent Sample 1D: TCR053 1322Violet Sample DateTime:Janvary 07,2019. 10:25 cS Sample 1D: 1901060 ‘Sample Description: Rexeed 255 Ulvafiter Date Received: January 08,2019 11:18 Amount Submitted: 100. Solids: WA mount analyzed: 100.0 L Receipt Temperature: 19.0 degC Preserved: No fnatyisStart: January 08,2019 11:50 ‘Aralyss Stop Oate: January 16,2019 16:15 alt Katherine Sayer, Pho. usatiier: # Primary Values 0 Visual presence of Amoeba Teophoroltes (0 for absence/1.0for presence) ‘Secondary Value © tiaegeria fowler ONA detection (0 for absence/10 for presence) Analisis Notes Active amosbatrophovotes not detected vu. N fowler PCR was negative, N.fwlei was not detected in the sample. lent Louisiana Department of Heath Analysis ‘Naeglria Fowler (Culeure/PCR) Project Name: City of Bossier LA1015004 (lent Sample 1D: TCR089 5311 Lantana Sample Oate/Tme:January 07, 2019 9:25 '8cs Sample: 1901061, Sample Description: Rexeed 255 Ulrafiter Date Receved: Janvary08, 2019 11:18 Amount Submited: 1001 Sols: Wy ‘Amount Aralzed: 100.0 L Receipt Temperature: 19.0 degC Preserved: No Analysis Star: January 08,2019 13:52 ‘Analisis Stop Oate: January 16,2019. 16:45 Analyst Katherine Sayer, Pho. Qualifier: # Primary Value: © Visual presence of Amoeba Trophozoites (0 for absenco/1.0 for presence) ‘Secondary Value: 110_Naegleria fowler DNA detection 0 for absence/.for presence ‘Analysis Notes: Active amoeba trophooites not visually detected. N. fowler PCR was postive. N. fowler ‘ONA was detected inthe sample. BCS LABORATORIES, INC. ~ GAINESVILLE 4609 NW 6ri StneET, StE,A, GAINESVILLE, FLORIDA 32609 (882) 377.9272, FAX. (852) 377.5630 FON wea2924,150/1 1702812008 .2422 ANAS) PADEMY 6803060, EPA FLO1147 whe @